CN115105424B

CN115105424B - Anti-wrinkle firming activator for skin care and preparation method thereof

Info

Publication number: CN115105424B
Application number: CN202211050550.5A
Authority: CN
Inventors: 刘颖
Original assignee: Changchun Aimeilian Cosmetics Co ltd
Current assignee: Changchun Aimeilian Cosmetics Co ltd
Priority date: 2022-08-31
Filing date: 2022-08-31
Publication date: 2022-11-15
Anticipated expiration: 2042-08-31
Also published as: CN115105424A

Abstract

The invention discloses an anti-wrinkle and firming activin for skin care and a preparation method thereof, wherein the anti-wrinkle and firming activin for skin care comprises the following raw materials in parts by weight: 3.5-6.5 parts of mannose erythritol ester, 3-5 parts of scutellarin, 1.8-3.2 parts of zein, 5-10 parts of lecithin, 0.1-0.5 part of active polypeptide, 7-12 parts of humectant, 1-5 parts of soothing agent, 1-2 parts of thickening agent and 70-90 parts of deionized water. The mannosylerythritol lipid added in the vitality element effectively stimulates dermal fibroblasts in the skin, promotes the dermal fibroblasts to secrete collagen, improves the vitality and elasticity of the skin, and reduces wrinkles. And scutellarin and zein are added for matching use, so that the zein improves the stability of the scutellarin, can improve the capability of an antioxidant component of the scutellarin in removing free radicals, improves the activity of skin cells, and realizes the effects of removing wrinkles and slowing down aging.

Description

Anti-wrinkle firming activator for skin care and preparation method thereof

Technical Field

The invention relates to the field of skin care products, in particular to an anti-wrinkle firming activator for skin care and a preparation method thereof.

Background

The skin is used as the first barrier of human body and has certain protection effect on human body. With the continuous improvement of living standard, people pay more and more attention to their face and image, and have higher and higher requirements on the functions of skin care products, and in addition to the most basic skin care effect, people tend to pursue the change of their skin by using skin care products, such as wrinkle reduction, skin tightening, etc., so that the skin is in a young state, and further, the anti-aging effect is realized.

With the increase of age, cell metabolism is slowed down, balance between cell proliferation and differentiation and cell aging and apoptosis is imbalanced, so that the scavenging capacity of excessive aged cells to in-vivo free radicals is reduced, in addition, the aged cells are continuously contacted with the outside, external pollution factors including ultraviolet rays, electromagnetic radiation and the like continuously generate free radicals in a human body, a large amount of free radicals and active oxygen are generated, the free radicals and the active oxygen can react with proteins in the skin, so that the proteins are subjected to steric isomerization, or the active groups in molecules are oxidized to cause the protein denaturation, and further the proteins lose the original functions, and the proteins comprise collagen and elastin in a dermis layer, so that the elasticity of the skin is reduced, and wrinkles are generated.

In order to prevent skin wrinkles caused by the reduction of collagen, collagen is directly added to many commercially available products, but these products have difficulty in percutaneous absorption of collagen, which is a polymer substance, because collagen itself is applied to the skin surface, and thus cannot exhibit a substantial wrinkle-improving effect.

At present, anti-wrinkle products are various, most of the anti-wrinkle products are chemical synthesis agents, and although the anti-wrinkle effects can be achieved in a short term, the anti-wrinkle products can cause another damage to the skin in a long term. The components such as vitamin E, tartaric acid, tocopherol and derivatives thereof are added into the existing anti-wrinkle products to increase the anti-wrinkle function of the skin care products, but after the skin care products with the components are opened, the effective components are easily oxidized and lose the anti-wrinkle function, the skin care products are required to be used as soon as possible after being opened, and the skin care products are not easy to store for a long time, so the use cost is increased. In some skin care products in the market, in order to achieve the effect of quickly removing wrinkles, hormones or other forbidden components are added into the skin care products, so that although the effect of quickly removing wrinkles can be achieved, serious hidden dangers are buried for skin health. The skin becomes more and more fragile with long-term use, and also causes skin laxity, edema, allergy and the like.

Therefore, the research of the skin care product which has safe components, can lighten wrinkles and has long-acting anti-wrinkle effect has very important significance.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide the anti-wrinkle and firming vitality element for skin care, which can effectively relieve skin aging, reduce skin wrinkles, and is safe and stable.

The invention also aims to provide a preparation method of the anti-wrinkle and firming activator for skin care.

One of the purposes of the invention is realized by adopting the following technical scheme:

the anti-wrinkle and tightening vitality element for skin care comprises the following raw materials in parts by weight: 3.5-6.5 parts of mannose erythritol ester, 3-5 parts of scutellarin, 1.8-3.2 parts of zein, 5-10 parts of lecithin, 0.1-0.5 part of active polypeptide, 7-12 parts of humectant, 1-5 parts of soothing agent, 1-2 parts of thickening agent and 70-90 parts of deionized water.

Preferably, the anti-wrinkle and tightening vitality element for skin care comprises the following raw materials in parts by weight: 5.5 parts of mannitose, 4 parts of scutellarin, 2.7 parts of zein, 7 parts of lecithin, 0.2 part of active polypeptide, 10 parts of humectant, 3 parts of soothing agent, 1.5 parts of thickening agent and 80 parts of deionized water.

Preferably, the active polypeptide is hexapeptide-1 and oligopeptide-3 which are composed of 1:1 parts by weight.

Preferably, the humectant is at least one of propylene glycol, butylene glycol, glycerin or sodium hyaluronate.

Preferably, the soothing agent is one of allantoin, dipotassium glycyrrhizinate and asiaticoside.

Preferably, the thickener is one of xanthan gum or carbomer.

The second purpose of the invention is realized by adopting the following technical scheme:

the preparation method of the anti-wrinkle and firming activin for skin care comprises the following steps:

(1) Adding the humectant and the thickening agent into deionized water, heating to 60-70 ℃, and fully stirring and uniformly mixing;

(2) Cooling the mixed material obtained in the step (1) to 37-42 ℃, adding lecithin, mannosylerythritol lipid and active polypeptide, and uniformly stirring;

(3) Keeping the temperature unchanged, adding scutellarin, zein and a soothing agent into the mixture obtained in the step (2), continuously stirring uniformly, and standing to restore the room temperature to obtain the product.

Compared with the prior art, the invention has the beneficial effects that: the invention provides an anti-wrinkle firming activin for skin care, wherein mannosylerythritol lipid added in the activin effectively stimulates dermal fibroblasts in skin, promotes the dermal fibroblasts to secrete collagen, improves skin vitality and elasticity, and reduces wrinkles.

The anti-wrinkle firming activin for skin care is also added with scutellarin and zein for matching use, and the zein improves the stability of the scutellarin, can improve the free radical scavenging capability of the scutellarin serving as an antioxidant component, improves the activity of skin cells, and realizes the effects of removing wrinkles and slowing down aging.

The components and the moisture retention agent and the active polypeptide in the activin have synergistic effect, so that the skin metabolic capacity is improved, the appearance of skin wrinkles is reduced or slowed down, the skin is kept alive, the effects of resisting wrinkles and resisting aging are realized, and the skin-nourishing cream has a good market prospect.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.

Example 1

The anti-wrinkle and firming activin for skin care is composed of the following raw materials in parts by weight: 5.5 parts of mannitol erythritol lipid, 4 parts of scutellarin, 2.7 parts of zein, 7 parts of lecithin, 0.1 part of hexapeptide, 0.1 part of oligopeptide-3, 10 parts of propylene glycol, 3 parts of allantoin, 1.5 parts of xanthan gum and 80 parts of deionized water.

(1) Adding propylene glycol and xanthan gum into deionized water, heating to 65 ℃, and fully stirring and uniformly mixing;

(2) Cooling the mixed material in the step (1) to 40 ℃, adding lecithin, mannosylerythritol lipid, hexapeptide-1 and oligopeptide-3, and uniformly stirring;

(3) Keeping the temperature unchanged, adding scutellarin, zein and allantoin into the mixture obtained in the step (2), continuously stirring uniformly, and standing to restore the room temperature to obtain the product.

Example 2

The anti-wrinkle and firming activin for skin care is composed of the following raw materials in parts by weight: 3.5 parts of mannitol erythritol lipid, 3 parts of scutellarin, 1.8 parts of zein, 5 parts of lecithin, 0.05 part of hexapeptide, 0.05 part of oligopeptide, 7 parts of sodium hyaluronate, 1 part of dipotassium glycyrrhizinate, 1 part of carbomer and 70 parts of deionized water.

(1) Adding sodium hyaluronate and carbomer into deionized water, heating to 60 deg.C, stirring thoroughly, and mixing;

(2) Cooling the mixed material in the step (1) to 37 ℃, adding lecithin, mannosylerythritol lipid, hexapeptide-1 and oligopeptide-3, and uniformly stirring;

(3) Keeping the temperature unchanged, adding scutellarin, zein and dipotassium glycyrrhizinate into the mixture obtained in the step (2), continuously stirring uniformly, and standing to restore the room temperature to obtain the product.

Example 3

The anti-wrinkle and firming activin for skin care is composed of the following raw materials in parts by weight: 6.5 parts of mannosylerythritol ester, 5 parts of scutellarin, 3.2 parts of zein, 10 parts of lecithin, 0.25 part of hexapeptide, 0.25 part of oligopeptide-3.25 part, 12 parts of butanediol, 5 parts of asiaticoside, 2 parts of xanthan gum and 90 parts of deionized water.

(1) Adding butanediol and xanthan gum into deionized water, heating to 70 ℃, and fully stirring and uniformly mixing;

(2) Cooling the mixed material in the step (1) to 42 ℃, adding lecithin, mannosylerythritol lipid, hexapeptide-1 and oligopeptide-3, and uniformly stirring;

(3) Keeping the temperature unchanged, adding scutellarin, zein and asiaticoside into the mixture obtained in the step (2), continuously stirring uniformly, and standing to restore the room temperature to obtain the product.

Comparative example 1

Comparative example 1 is an anti-wrinkle and tightening activator for skin care, which is composed of the following raw materials in parts by weight: 4 parts of scutellarin, 2.7 parts of zein, 7 parts of lecithin, 1.1 parts of hexapeptide, 3.1 parts of oligopeptide, 10 parts of propylene glycol, 3 parts of allantoin, 1.5 parts of xanthan gum and 80 parts of deionized water. That is, the difference from example 1 is that the mannosylerythritol lipids are omitted, and the others are the same as example 1.

Comparative example 2

Comparative example 2 is an anti-wrinkle and tightening activator for skin care, which is composed of the following raw materials in parts by weight: 5.5 parts of mannosylerythritol lipid, 7 parts of lecithin, 0.1 part of hexapeptide, 3.1 parts of oligopeptide, 10 parts of propylene glycol, 3 parts of allantoin, 1.5 parts of xanthan gum and 80 parts of deionized water. Namely, the difference from example 1 is that scutellarin and zein are omitted, and the others are the same as those in example 1.

Comparative example 3

Comparative example 3 is an anti-wrinkle and tightening activator for skin care, which is composed of the following raw materials in parts by weight: 5.5 parts of mannosylerythritol lipid, 2.7 parts of zein, 7 parts of lecithin, 0.1 part of hexapeptide, 0.1 part of oligopeptide-3, 10 parts of propylene glycol, 3 parts of allantoin, 1.5 parts of xanthan gum and 80 parts of deionized water. Namely, the difference from example 1 is that scutellarin is omitted, and the others are the same as example 1.

Comparative example 4

Comparative example 4 is an anti-wrinkle and tightening activator for skin care, which is composed of the following raw materials in parts by weight: 5.5 parts of mannosylerythritol lipid, 4 parts of scutellarin, 7 parts of lecithin, 1.1 parts of hexapeptide, 0.1 part of oligopeptide, 10 parts of propylene glycol, 3 parts of allantoin, 1.5 parts of xanthan gum and 80 parts of deionized water. Namely, the difference from example 1 is that zein was omitted, and the others are the same as example 1.

Test example 1

Taking P3 generation human dermal fibroblasts at logarithmic growth phase, resuspending in DMEM/F12 medium supplemented with 10% FBS at a cell density of 1X 10 ⁵ Inoculating the cells/mL into 12-well plates, culturing at 2mL per well for 24h, and setting experimental group and control group respectively, each group having 6 multiple wells, wherein the experimental group 1 is added with mannosylerythritol lipid 5ng/mL, the experimental group 2 is added with mannosylerythritol lipid 10ng/mL, the experimental group 3 is added with mannosylerythritol lipid 15ng/mL, the control group is not added, and placing at 37 deg.C and 5% CO ₂ The cells were cultured for 3d in the incubator of (1), and the cell count was performed by trypan blue staining, and the cell growth factor was calculated by taking the average of three wells. The results are shown in Table 1.

Cell supernatants from the remaining 3 wells of each group were collected, and the expression level of Type I collagen in the supernatants was measured using Procollagen Type I C-peptide (PIP) ELA kit, with the results shown in table 1.

TABLE 1

Group of	Fold of proliferation	Type I collagen expression level (ng/mL)
			Experimental group 1	22.74	161.26±5.44
Experimental group 2	28.49	184.53±5.59
			Experimental group 3	18.32	121.39±4.25
Control group	15.86	93.62±2.37

As can be seen from Table 1, the concentration of the mannosylerythritol lipid in the range of 5-15ng/mL has different degrees of promotion effects on the proliferation of human dermal fibroblasts and the expression of collagen, wherein the effect is the best when the concentration of the mannosylerythritol lipid is 10 ng/mL. Therefore, the mannosylerythritol lipid can promote the proliferation of human dermal fibroblasts, improve the content of collagen in skin, reduce the generation of skin wrinkles and delay skin aging.

Test example 2

And (3) antioxidant test: dissolving DPPH in ethanol, preparing 0.1mg/L DPPH solution, dissolving scutellarin in 0.02mmol/L sodium hydroxide solution, adding distilled water to dilute to 100mmol/mL, wherein the experimental group 1 is 100mmol/mL scutellarin, the experimental group 2 is prepared by adding zein into 100mmol/mL scutellarin, the zein concentration is 40mmol/mL, the experimental group 3 is prepared by adding zein (the solvent is 80% ethanol) in the concentration of 40mmol/mL, and the control group is 80% ethanol. 50 mu L of each sample is added into 50 mu L of 80% ethanol, then is rapidly added into 150 mu L of the PPH solution, the reaction is carried out for 30min at room temperature, the absorbance A at 517nm is detected by a microplate reader, and the clearance is calculated, and the result is shown in Table 2.

Clearance (%) = (1- (S-B)/C) × 100%.

Wherein C is the absorbance value of a blank group without adding DPPH solution in a detection sample, S is the absorbance value of the detection sample (the sample to be detected and DPPH solution in experiment groups 1 to 3), and B is the absorbance value of a control group (80% ethanol and DPPH solution).

TABLE 2

Group of	Clearance (%)
		Experimental group 1	58.45±5.67
Experimental group 2	71.29±8.61
		Experimental group 3	0.17±0.08

As can be seen from Table 2, scutellarin has better clearance rate, and the addition of zein is helpful for improving the oxidation resistance of scutellarin. Therefore, the zein and the scutellarin are added into the activin in a matching way, so that the oxidation resistance of the product can be further improved, the wrinkles on the surface of the skin are reduced, and the skin aging is delayed.

Test example 3

The activin of example 1 and comparative example 4 was placed in a seal at 45 ℃ for 28 days and the products were used in the following tests as comparative example 5 (example 1 product after 28d placement) and comparative example 6 (comparative example 4 product after 28d placement), respectively.

90 female volunteers between 30-45 ages are randomly divided into 9 groups, 10 persons in each group, the forehead of the volunteer is respectively coated with the products of examples 1-3 and comparative examples 1-6, the products are respectively coated once every morning and evening, the products are continuously used for 28 days, and other skin care products are not used in the period. The test was carried out before the use of the product (0 d), on day 28, respectively. The volunteers cleaned their faces before examination, examined the same area three times using a VISIA facial image analyzer under an ambient condition of 25 ℃ and 50% humidity, averaged, and the percentage reduction in the 28 th skin wrinkle area compared to that before use was calculated, with the results shown in table 3.

TABLE 3

Group of	Percent reduction in skin wrinkle area (%)
		Example 1	21.47
Example 2	20.04
		Example 3	21.12
Comparative example 1	8.47
		Comparative example 2	12.62
Comparative example 3	11.83
		Comparative example 4	16.37
Comparative example 5	20.06
		Comparative example 6	3.73

It can be seen from table 3 that the activin of examples 1 to 3 has better anti-wrinkle effect, and the components of the activin are adjusted in comparative examples 1 to 4, so that the anti-wrinkle capability of the product is reduced, further, the synergistic effect of the mannosylerythritol lipid, scutellarin and zein added in the invention is further illustrated, so that the anti-wrinkle effect of the activin is improved, and the skin-tightening and anti-aging effects are achieved. The comparative example 5 has little effect compared with the example 1, which shows that the product of the activin of the invention has stable performance. Compared with comparative example 4, the anti-wrinkle effect is obviously reduced, which shows that the addition of zein is beneficial to improving the stability of scutellarin and maintaining better antioxidant activity, thereby exerting the effect of reducing skin wrinkles.

Test example 4

Selecting 30 female volunteers between ages of 30-45, randomly dividing into 3 groups, and randomly dividing each group into 10 persons, respectively placing the activins of examples 1-3 into spot testers by a closed spot test method, applying medical adhesive tapes to the inner sides of the left upper arms of the volunteers, removing after 24 hours, observing skin reactions 0.5, 24 and 48 hours after the spot pastes are removed, and recording the results according to the test method and the skin reaction grading standard in technical Specification for safety of cosmetics (2015 edition). The grading standards are shown in Table 4, and the test results are shown in Table 5.

TABLE 4

Extent of reaction	Grade	Skin reactions
			-	0	Negative reaction, no skin discomfort
±	1	Suspicious reaction, only faint erythema
			+	2	Weak positive reaction: erythema, infiltrations, edema, and possibly papules
++	3	Strong positive reaction: erythema, infiltration, edema, pimple, herpes, reaction beyond the area of the subject
			+++	4	Extremely strong positive reaction: the skin has obvious erythema, severe infiltration, edema and herpes fusiformis, and the reaction exceeds the tested area

TABLE 5

Grade	Negative reaction (/ human)
		Example 1	10
Example 2	10
		Example 3	10

As can be seen from tables 4 and 5, the activin of the invention has no adverse reaction, which indicates that the ingredients are safe and mild and have no irritation to skin.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. The anti-wrinkle and firming activator for skin care is characterized by comprising the following raw materials in parts by weight: 3.5-6.5 parts of mannose erythritol ester, 3-5 parts of scutellarin, 1.8-3.2 parts of zein, 5-10 parts of lecithin, 0.1-0.5 part of active polypeptide, 7-12 parts of humectant, 1-5 parts of soothing agent, 1-2 parts of thickening agent and 70-90 parts of deionized water.

2. The anti-wrinkle and anti-firmness activator for skin care according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 5.5 parts of mannitose, 4 parts of scutellarin, 2.7 parts of zein, 7 parts of lecithin, 0.2 part of active polypeptide, 10 parts of humectant, 3 parts of soothing agent, 1.5 parts of thickening agent and 80 parts of deionized water.

3. The anti-wrinkle and anti-stress activator for skin care according to claim 1, wherein the active polypeptide is hexapeptide-1 and oligopeptide-3, and the active polypeptide is 1:1 by weight.

4. The anti-wrinkle and anti-stress agent for skin care according to claim 1, wherein the humectant is at least one of propylene glycol, butylene glycol, glycerin, or sodium hyaluronate.

5. The anti-wrinkle and anti-stress active substance according to claim 1, wherein the soothing agent is one of allantoin, dipotassium glycyrrhizinate and asiaticoside.

6. The anti-wrinkle and anti-stress activator for skin care according to claim 1, wherein the thickener is one of xanthan gum or carbomer.

7. The method for preparing the anti-wrinkle and anti-stress activator for skin care according to any one of claims 1 to 6, comprising the steps of:

(1) Adding the humectant and the thickener into deionized water, heating to 60-70 ℃, and fully stirring and uniformly mixing;

(3) Keeping the temperature unchanged, adding scutellarin, zein and a soothing agent into the mixture obtained in the step (2), continuously stirring uniformly, and standing to recover the room temperature to obtain the product.