CN114835783B - NCgl2747基因突变体及其在制备L-赖氨酸中的应用 - Google Patents

NCgl2747基因突变体及其在制备L-赖氨酸中的应用 Download PDF

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CN114835783B
CN114835783B CN202210616402.9A CN202210616402A CN114835783B CN 114835783 B CN114835783 B CN 114835783B CN 202210616402 A CN202210616402 A CN 202210616402A CN 114835783 B CN114835783 B CN 114835783B
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ncgl2747
gene
seq
leu
ala
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CN114835783A (zh
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孟刚
周晓群
魏爱英
赵春光
马风勇
马文有
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Ningxia Eppen Biotech Co Ltd
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Ningxia Eppen Biotech Co Ltd
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Priority to CN202210616402.9A priority Critical patent/CN114835783B/zh
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Priority to JP2024571087A priority patent/JP2025518814A/ja
Priority to EP23814743.3A priority patent/EP4534550A4/en
Priority to PCT/CN2023/084970 priority patent/WO2023231547A1/zh
Priority to KR1020247043577A priority patent/KR20250019107A/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/265—Micrococcus
    • C12R2001/28—Micrococcus glutamicus ; Corynebacterium glutamicum

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN202210616402.9A 2022-06-01 2022-06-01 NCgl2747基因突变体及其在制备L-赖氨酸中的应用 Active CN114835783B (zh)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN202210616402.9A CN114835783B (zh) 2022-06-01 2022-06-01 NCgl2747基因突变体及其在制备L-赖氨酸中的应用
JP2024571087A JP2025518814A (ja) 2022-06-01 2023-03-30 ＮＣｇｌ２７４７遺伝子変異体およびＬ－リジン調製におけるその使用
EP23814743.3A EP4534550A4 (en) 2022-06-01 2023-03-30 MUTANT OF THE NCGL2747 GENE AND ITS USE IN THE PREPARATION OF L-LYSINE
PCT/CN2023/084970 WO2023231547A1 (zh) 2022-06-01 2023-03-30 NCgl2747基因突变体及其在制备L-赖氨酸中的应用
KR1020247043577A KR20250019107A (ko) 2022-06-01 2023-03-30 NCgl2747 유전자 돌연변이체 및 이의 L-라이신 제조에서의 응용

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210616402.9A CN114835783B (zh) 2022-06-01 2022-06-01 NCgl2747基因突变体及其在制备L-赖氨酸中的应用

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CN114835783A CN114835783A (zh) 2022-08-02
CN114835783B true CN114835783B (zh) 2024-06-25

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EP (1) EP4534550A4 (https=)
JP (1) JP2025518814A (https=)
KR (1) KR20250019107A (https=)
CN (1) CN114835783B (https=)
WO (1) WO2023231547A1 (https=)

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CN115820706B (zh) * 2022-12-29 2025-07-29 宁夏伊品生物科技股份有限公司 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用
CN117264034B (zh) * 2023-11-21 2024-02-06 内蒙古伊品生物科技有限公司 Bbd29_09715基因突变体及其在制备l-谷氨酸中的应用
CN120058884A (zh) * 2023-11-30 2025-05-30 宁夏伊品生物科技股份有限公司 细胞衰亡相关蛋白在制备l-赖氨酸中的应用
CN118531015A (zh) * 2024-06-06 2024-08-23 宁夏伊品生物科技股份有限公司 PNcgl1855（A244C）启动子及其相关生物材料在制备L-赖氨酸中的应用
CN118374533B (zh) * 2024-06-24 2024-08-20 东晓生物科技股份有限公司 一种产l-赖氨酸的谷氨酸棒杆菌的构建方法及应用
CN119708180B (zh) * 2024-12-31 2026-03-10 宁夏伊品生物科技股份有限公司 NCgl0762蛋白质及其突变体在生产L-异亮氨酸中的应用

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JPH06102028B2 (ja) * 1985-10-04 1994-12-14 協和醗酵工業株式会社 アミノ酸の製造法
SK285201B6 (sk) * 1996-12-05 2006-08-03 Ajinomoto Co., Inc. Rekombinantná DNA, autonómne reprodukovaná v bunkách koryneformnej baktérie, koryneformná baktéria a spôsob výroby L-lyzínu, vektor pVK7
MXPA01013123A (es) * 1999-06-25 2002-06-21 Basf Ag Genes de corynebacterium glutamicum que codifican proteinas de via metabolica.
DE102005019967A1 (de) * 2005-04-29 2006-11-02 Forschungszentrum Jülich GmbH Verfahren zur fermentativen Herstellung von L-Aminosäuren
WO2008033001A1 (en) * 2006-09-15 2008-03-20 Cj Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same
JP6335196B2 (ja) 2013-02-08 2018-05-30 ニンシャー エッペン バイオテック カンパニー リミテッド アコニターゼ遺伝子および／またはその調節エレメントの改変により、ｌ−リジンを生産する方法
BR112018011503A2 (pt) * 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
EP3533875B1 (en) * 2016-09-01 2023-02-15 Ningxia Eppen Biotech Co. Ltd Corynebacterium for producing l-lysine by fermentation
KR20200026881A (ko) * 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
CN110862940B (zh) * 2019-11-20 2021-06-01 金河生物科技股份有限公司 一种谷氨酸棒杆菌工程菌及其在制备l-色氨酸中的应用
CN113249286B (zh) * 2021-05-25 2023-12-08 洛阳华荣生物技术有限公司 一种构建l-肌氨酸生产菌的方法

Non-Patent Citations (2)

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Title
Improvement of L-lysine production combines with minimization of by-products synthesis in Corynebacterium glutamicum.;Xu等;JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY;第89卷(第12期);参见第1924-1933页 *
基于代谢工程选育谷氨酸棒杆菌L-赖氨酸高产菌.;徐建中;CNKI博士学位论文全文库;参见摘要 *

Also Published As

Publication number Publication date
JP2025518814A (ja) 2025-06-19
KR20250019107A (ko) 2025-02-07
CN114835783A (zh) 2022-08-02
EP4534550A4 (en) 2026-03-25
EP4534550A1 (en) 2025-04-09
WO2023231547A1 (zh) 2023-12-07

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