CN1148230C - 复合人工皮肤的制备方法 - Google Patents
复合人工皮肤的制备方法 Download PDFInfo
- Publication number
- CN1148230C CN1148230C CNB011070994A CN01107099A CN1148230C CN 1148230 C CN1148230 C CN 1148230C CN B011070994 A CNB011070994 A CN B011070994A CN 01107099 A CN01107099 A CN 01107099A CN 1148230 C CN1148230 C CN 1148230C
- Authority
- CN
- China
- Prior art keywords
- solution
- preparation
- cell
- matrix gel
- artificial skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 150000001875 compounds Chemical class 0.000 title claims description 18
- 239000011159 matrix material Substances 0.000 claims abstract description 27
- 102000008186 Collagen Human genes 0.000 claims abstract description 20
- 108010035532 Collagen Proteins 0.000 claims abstract description 20
- 229920001436 collagen Polymers 0.000 claims abstract description 20
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 9
- 229920002101 Chitin Polymers 0.000 claims abstract description 9
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 9
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 9
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229920001287 Chondroitin sulfate Polymers 0.000 claims abstract description 8
- 229940059329 chondroitin sulfate Drugs 0.000 claims abstract description 8
- 102000016942 Elastin Human genes 0.000 claims abstract description 7
- 108010014258 Elastin Proteins 0.000 claims abstract description 7
- 229920002971 Heparan sulfate Polymers 0.000 claims abstract description 7
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229920002549 elastin Polymers 0.000 claims abstract description 7
- 239000000835 fiber Substances 0.000 claims abstract description 7
- 210000003491 skin Anatomy 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 28
- 210000004027 cell Anatomy 0.000 claims description 25
- 210000002615 epidermis Anatomy 0.000 claims description 18
- 210000002950 fibroblast Anatomy 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 244000309466 calf Species 0.000 claims description 9
- 210000002966 serum Anatomy 0.000 claims description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Natural products CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000006143 cell culture medium Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 6
- 230000012010 growth Effects 0.000 claims description 5
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 3
- 102000004877 Insulin Human genes 0.000 claims description 3
- 108090001061 Insulin Proteins 0.000 claims description 3
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- 102000057297 Pepsin A Human genes 0.000 claims description 3
- 108090000284 Pepsin A Proteins 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- -1 acetic acid compound Chemical class 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 230000010261 cell growth Effects 0.000 claims description 3
- 230000029087 digestion Effects 0.000 claims description 3
- 239000003102 growth factor Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 229940125396 insulin Drugs 0.000 claims description 3
- 229940049954 penicillin Drugs 0.000 claims description 3
- 229940111202 pepsin Drugs 0.000 claims description 3
- 239000012460 protein solution Substances 0.000 claims description 3
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 229960005322 streptomycin Drugs 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- 239000000645 desinfectant Substances 0.000 claims description 2
- 229960000890 hydrocortisone Drugs 0.000 claims description 2
- 230000002924 anti-infective effect Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 238000002054 transplantation Methods 0.000 abstract description 2
- 239000000853 adhesive Substances 0.000 abstract 1
- 230000001070 adhesive effect Effects 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 17
- 230000002500 effect on skin Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 238000010562 histological examination Methods 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000515 collagen sponge Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 229920000954 Polyglycolide Polymers 0.000 description 1
- 208000028990 Skin injury Diseases 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 239000000512 collagen gel Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 239000002254 cytotoxic agent Substances 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229940014259 gelatin Drugs 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 210000002570 interstitial cell Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229950008885 polyglycolic acid Drugs 0.000 description 1
- 239000004633 polyglycolic acid Substances 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 210000002107 sheath cell Anatomy 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011070994A CN1148230C (zh) | 2001-02-06 | 2001-02-06 | 复合人工皮肤的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB011070994A CN1148230C (zh) | 2001-02-06 | 2001-02-06 | 复合人工皮肤的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1310027A CN1310027A (zh) | 2001-08-29 |
CN1148230C true CN1148230C (zh) | 2004-05-05 |
Family
ID=4656052
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB011070994A Expired - Lifetime CN1148230C (zh) | 2001-02-06 | 2001-02-06 | 复合人工皮肤的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1148230C (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100462059C (zh) * | 2005-05-25 | 2009-02-18 | 中国人民解放军第四军医大学口腔医院 | 修复皮肤缺损的人工皮肤的制备方法 |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8501396B2 (en) | 2001-11-05 | 2013-08-06 | Medgenics Medical Israel Ltd. | Dermal micro-organs, methods and apparatuses for producing and using the same |
US7468242B2 (en) | 2001-11-05 | 2008-12-23 | Medgenics, Inc. | Dermal micro organs, methods and apparatuses for producing and using the same |
KR101134434B1 (ko) * | 2003-05-01 | 2012-04-09 | 메드제닉스 인코포레이티드 | 진피 미세 기관, 그를 생산 및 이용하기 위한 방법 및 장치 |
CN1297324C (zh) * | 2003-07-25 | 2007-01-31 | 吕伟光 | 组织工程自体复合皮肤及其制备方法 |
CN1314460C (zh) * | 2004-07-15 | 2007-05-09 | 浙江大学 | 将水凝胶复合到多孔组织工程支架中的方法 |
CN100522264C (zh) * | 2006-09-14 | 2009-08-05 | 中国人民解放军第三军医大学第三附属医院 | 一种含基底膜结构的双层组织工程皮肤制备方法 |
CN101297981B (zh) * | 2008-07-03 | 2011-11-02 | 中国检验检疫科学研究院 | 组织工程皮肤构建方法及用其制备的组织工程皮肤 |
CN101361990B (zh) * | 2008-09-03 | 2012-09-19 | 陕西瑞盛生物科技有限公司 | 一种双层人工皮肤及其制备方法 |
CN101361989B (zh) * | 2008-09-03 | 2012-09-19 | 陕西瑞盛生物科技有限公司 | 双层膜状组织修补材料及其制备方法 |
KR101739330B1 (ko) * | 2010-10-29 | 2017-05-24 | (주)아모레퍼시픽 | 진피 모사체를 이용한 피부 탄력 증진력 평가법 |
CN104971382B (zh) * | 2014-04-01 | 2017-08-15 | 上海合锐生物技术有限公司 | 一种用无血清和牛垂体提取物培养液构建的创口贴式人工活性组织及其构建方法 |
CN104068945B (zh) * | 2014-06-27 | 2016-11-16 | 深圳齐康医疗器械有限公司 | 一种人工皮肤及其制备方法 |
CN107320781B (zh) * | 2017-07-11 | 2020-03-10 | 广州润虹医药科技股份有限公司 | 含活细胞的组织工程皮肤及其制备方法 |
CN109550080A (zh) * | 2019-01-24 | 2019-04-02 | 中国人民解放军陆军特色医学中心 | 一种人工双层皮肤及其制备方法 |
CN113750286B (zh) * | 2021-09-30 | 2022-11-29 | 振德医疗用品股份有限公司 | 一种皮肤创面覆盖膜及其制备方法 |
-
2001
- 2001-02-06 CN CNB011070994A patent/CN1148230C/zh not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100462059C (zh) * | 2005-05-25 | 2009-02-18 | 中国人民解放军第四军医大学口腔医院 | 修复皮肤缺损的人工皮肤的制备方法 |
Also Published As
Publication number | Publication date |
---|---|
CN1310027A (zh) | 2001-08-29 |
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Owner name: WEILIBAO BIO-TECHNOLOGY (CHENGDU) CO., LTD. Free format text: FORMER OWNER: NO.3 HOSPITAL ATTACHED TO NO.3 MILITARY MEDICAL UNIV., P.L.A.;NO.3 HOSPITAL ATTACHED TO NO.3 MILITAR Effective date: 20150121 |
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Effective date of registration: 20150121 Address after: 611731, No. 9 South Road, Chengdu high tech Zone, Sichuan, China Patentee after: Weili biological technology (Chengdu) Co., Ltd. Address before: Chongqing city Yuzhong District Daping 400042 Yangtze River Branch No. 10 Patentee before: The Third Affiliated Hospital of Third Military Medical University of PLA |
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Effective date of registration: 20190522 Address after: 610015 No. 9 Herui South Road, West Park of Chengdu High-tech Zone, Sichuan Province Patentee after: Weili biological technology (Chengdu) Co., Ltd. Address before: 400041 No. 70-1 and No. 70-2 Keyuan Fourth Street, Jiulongpo District, Chongqing Patentee before: Chongqing Weili Biological Technology Co., Ltd. |
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Granted publication date: 20040505 |