CN114616327A - 甲醛转化突变蛋白及其应用 - Google Patents

甲醛转化突变蛋白及其应用 Download PDF

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CN114616327A
CN114616327A CN202180005890.3A CN202180005890A CN114616327A CN 114616327 A CN114616327 A CN 114616327A CN 202180005890 A CN202180005890 A CN 202180005890A CN 114616327 A CN114616327 A CN 114616327A
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formaldehyde
dihydroxyacetone
mutein
metal hydroxide
seq
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CN114616327B (zh
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朱蕾蕾
李天真
唐梓静
谭子瑊
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Tianjin Institute of Industrial Biotechnology of CAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J23/00—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00
    • B01J23/02—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of the alkali- or alkaline earth metals or beryllium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C12P7/28—Acetone-containing products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11023—Flavonol synthase (1.14.11.23)

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  • Materials Engineering (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
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Abstract

本发明公开了甲醛转化突变蛋白及其应用，所述突变蛋白可催化甲醛制备1,3‑二羟基丙酮，并进一步通过1,3‑二羟基丙酮合成乳酸和乙醇酸。还公开了一种合成乳酸和乙醇酸的方法。本发明的突变蛋白能提高甲醛到1,3‑二羟基丙酮的催化效率，可以在温和条件下进行。

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PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN202180005890.3A 2020-02-24 2021-02-09 甲醛转化突变蛋白及其应用 Active CN114616327B (zh)

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Application Number Priority Date Filing Date Title
CN202010113441 2020-02-24
CN2020101134418 2020-02-24
CN202010984037.8A CN112852766B (zh) 2020-02-24 2020-09-17 一种合成乳酸的方法
CN2020109840378 2020-09-17
CN202010983978.XA CN112852765B (zh) 2020-02-24 2020-09-17 甲醛转化突变蛋白及其应用
CN202010983978X 2020-09-17
PCT/CN2021/076379 WO2021169814A1 (zh) 2020-02-24 2021-02-09 甲醛转化突变蛋白及其应用

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CN202010984037.8A Active CN112852766B (zh) 2020-02-24 2020-09-17 一种合成乳酸的方法
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CN114591938B (zh) * 2022-04-07 2023-07-25 山东金城医药研究院有限公司 羧化酶突变体及其制备方法和应用
CN115109770B (zh) * 2022-06-30 2023-09-05 中国科学院天津工业生物技术研究所 苯甲醛裂解酶突变体及在制备1，4-二羟基-2-丁酮中的应用

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CN106916794A (zh) * 2017-02-22 2017-07-04 中国科学院天津工业生物技术研究所 催化甲醛合成羟基乙醛的酶及其应用
CN108118037A (zh) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 一种耐热性提高的葡萄糖氧化酶突变体

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JP2007228927A (ja) * 2006-03-02 2007-09-13 Kaneka Corp グリコール酸の製造方法
AT503802B1 (de) * 2006-07-26 2008-01-15 Vtu Engineering Planungs Und B Verfahren zur herstellung von milchsäure bzw. eines salzes davon
US9200288B2 (en) * 2010-04-27 2015-12-01 The Regents Of The University Of California Production of 1,4-butanediol by recombinant microorganisms
EP3325608B1 (en) * 2015-07-21 2021-11-24 The Governing Council of the University of Toronto Methods and microorganisms for the production of 1,3-butanediol
CN105132400B (zh) * 2015-07-24 2018-10-12 中国科学院天津工业生物技术研究所 具有催化甲醛合成1,3-二羟基丙酮功能的酶及其制备方法
CN105777523B (zh) * 2016-04-07 2018-05-25 农业部环境保护科研监测所 一种温和条件下由糖类制备乳酸的方法
WO2018038667A1 (en) * 2016-08-25 2018-03-01 Medivir Ab Respiratory syncytial virus inhibitors
BR112019009884B1 (pt) * 2016-11-24 2022-08-02 Haldor Topsøe A/S Método para produção catalítica de ácido glicólico e/ou glicolato
CN107699536B (zh) * 2017-11-27 2021-02-05 南京工业大学 一种基因工程菌及其在生产d-1,2,4-丁三醇中的应用
CN115725524A (zh) * 2018-05-31 2023-03-03 中国科学院天津工业生物技术研究所 羰基还原酶突变体及其在环戊二酮类化合物还原中的应用

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CN106916794A (zh) * 2017-02-22 2017-07-04 中国科学院天津工业生物技术研究所 催化甲醛合成羟基乙醛的酶及其应用
CN108713060A (zh) * 2017-02-22 2018-10-26 中国科学院天津工业生物技术研究所 催化甲醛合成羟基乙醛和/或1,3-二羟基丙酮的酶及其应用
US20200024590A1 (en) * 2017-02-22 2020-01-23 Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences Enzyme for synthesizing hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde, and applications thereof

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CN112852766B (zh) 2022-03-25
WO2021169814A1 (zh) 2021-09-02
CN112852765B (zh) 2021-11-12
CN114616327B (zh) 2024-03-22
CN112852765A (zh) 2021-05-28
CN113832120B (zh) 2024-04-26
CN113832120A (zh) 2021-12-24

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