CN109251882B - 一株异源表达耐热腈水合酶的大肠杆菌重组菌及其应用 - Google Patents
一株异源表达耐热腈水合酶的大肠杆菌重组菌及其应用 Download PDFInfo
- Publication number
- CN109251882B CN109251882B CN201811438328.6A CN201811438328A CN109251882B CN 109251882 B CN109251882 B CN 109251882B CN 201811438328 A CN201811438328 A CN 201811438328A CN 109251882 B CN109251882 B CN 109251882B
- Authority
- CN
- China
- Prior art keywords
- nitrile hydratase
- glu
- leu
- escherichia coli
- val
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/10—Nitrogen as only ring hetero atom
- C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01084—Nitrile hydratase (4.2.1.84)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
P1 | 5'-TATA<u>CATATG</u>AATGGCATTCATGATTTAGG-3' |
P2 | 5'-GCTC<u>GAATTC</u>TTAAAAAAACTCATCGC-3' |
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811438328.6A CN109251882B (zh) | 2018-11-28 | 2018-11-28 | 一株异源表达耐热腈水合酶的大肠杆菌重组菌及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811438328.6A CN109251882B (zh) | 2018-11-28 | 2018-11-28 | 一株异源表达耐热腈水合酶的大肠杆菌重组菌及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109251882A CN109251882A (zh) | 2019-01-22 |
CN109251882B true CN109251882B (zh) | 2020-12-29 |
Family
ID=65042199
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811438328.6A Active CN109251882B (zh) | 2018-11-28 | 2018-11-28 | 一株异源表达耐热腈水合酶的大肠杆菌重组菌及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109251882B (zh) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021081868A1 (zh) * | 2019-10-31 | 2021-05-06 | 江南大学 | 一种温泉热碱芽孢杆菌来源的腈水合酶的突变体 |
CN113151234B (zh) * | 2021-04-13 | 2022-08-12 | 浙江工业大学 | 腈水合酶赖氨酸突变体hba-k2h2r、编码基因及应用 |
CN113151233B (zh) * | 2021-04-13 | 2022-08-12 | 浙江工业大学 | 腈水合酶赖氨酸突变体hba-k2h2、编码基因及应用 |
CN113122526B (zh) * | 2021-04-14 | 2023-09-22 | 浙江工业大学 | 腈水合酶赖氨酸突变体hba-k1、编码基因及应用 |
CN112941062B (zh) * | 2021-04-14 | 2023-04-28 | 浙江工业大学 | 腈水合酶赖氨酸突变体hba-k2h1、编码基因及应用 |
CN113846040B (zh) * | 2021-09-10 | 2023-07-25 | 江南大学 | 协同两类腈水合酶催化烟酰胺及丙烯酰胺生物合成的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103320458B (zh) * | 2012-09-18 | 2016-01-20 | 江南大学 | 一种来源于放线菌的腈水合酶基因在大肠杆菌中高效表达的方法 |
CN104561065A (zh) * | 2014-12-30 | 2015-04-29 | 杭州师范大学 | 一种耐热重组腈水合酶基因、编码酶、工程菌及其应用 |
CN104561064B (zh) * | 2014-12-30 | 2018-05-22 | 杭州师范大学 | 一种腈水合酶基因、编码酶、载体、工程菌及其制备酰胺化合物的应用 |
CN104830747B (zh) * | 2015-05-13 | 2018-01-16 | 江南大学 | 一种高效表达高分子量型腈水合酶的基因工程菌及其应用 |
-
2018
- 2018-11-28 CN CN201811438328.6A patent/CN109251882B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN109251882A (zh) | 2019-01-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109251882B (zh) | 一株异源表达耐热腈水合酶的大肠杆菌重组菌及其应用 | |
CN109593750B (zh) | 一种腈水合酶突变体、含该突变体的基因工程菌及其应用 | |
CN110938616B (zh) | 一种温泉热碱芽孢杆菌来源的腈水合酶的突变体 | |
CN108795916B (zh) | 一种赖氨酸脱羧酶突变体、其编码基因及其表达和应用 | |
CN108103120B (zh) | 一种双酶偶联全细胞催化马来酸合成l-天冬氨酸的方法 | |
US11332731B2 (en) | Nitrile hydratase mutant, genetically engineered bacterium containing mutant and applications thereof | |
CN112877307B (zh) | 一种氨基酸脱氢酶突变体及其应用 | |
CN112322606B (zh) | 一种腈水合酶突变体及其应用 | |
CN111471668A (zh) | 一种腈水解酶突变体及其在制备1-氰基环己基乙酸中的应用 | |
CN109251881A (zh) | 一株异源表达腈水合酶的大肠杆菌重组菌及其应用 | |
CN113337495B (zh) | 一种提高唾液酸产量的方法与应用 | |
CN113462677B (zh) | 一种α亚基突变的腈水合酶突变体及其应用 | |
CN110592119A (zh) | 一种来源于类芽孢杆菌新型普鲁兰酶及其基因与应用 | |
CN113846040B (zh) | 协同两类腈水合酶催化烟酰胺及丙烯酰胺生物合成的方法 | |
CN110804602B (zh) | 一种L-天冬氨酸β-脱羧酶突变体及其应用 | |
CN111004794B (zh) | 一种热稳定性提高的枯草蛋白酶e突变体及其应用 | |
CN113215120A (zh) | 重组大肠杆菌转化生产反式-4-羟基-l-脯氨酸的方法 | |
CN110699345A (zh) | 一种卤醇脱卤酶突变体及其应用 | |
CN113564138B (zh) | 一种二氨基庚二酸脱氢酶突变体及其应用 | |
CN114621944B (zh) | 酶活提高的精氨酸脱亚胺酶突变体 | |
CN114196659B (zh) | 酰胺酶突变体、编码基因、工程菌及其应用 | |
CN110241094B (zh) | 一种偶氮还原酶及其应用 | |
CN107201355B (zh) | 一种高立体选择性苯丙氨酸脱氨酶突变体及其应用 | |
CN114854717A (zh) | 一种脂肪酶及其编码基因与应用 | |
CN114107270A (zh) | 一种L-天冬氨酸β-脱羧酶突变体 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhou Zhemin Inventor after: Liu Zhongmei Inventor after: Ma Dong Inventor after: Cui Wenjing Inventor after: Zhou Li Inventor after: Guo Junling Inventor after: Zhang Sailan Inventor before: Zhou Zhemin Inventor before: Liu Zhongmei Inventor before: Cui Wenjing Inventor before: Zhou Li Inventor before: Guo Junling Inventor before: Zhang Sailan |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20211029 Address after: 214000 1800 Lihu Avenue, Binhu District, Wuxi, Jiangsu Patentee after: Jiangnan University Patentee after: Wuxi xinchenyu Bioengineering Co.,Ltd. Address before: No. 1800 Lihu Avenue, Wuxi City, Jiangsu Province Patentee before: Jiangnan University |