CN114502731A - 基于转座子的免疫细胞的修饰 - Google Patents

基于转座子的免疫细胞的修饰 Download PDF

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CN114502731A
CN114502731A CN202080027488.0A CN202080027488A CN114502731A CN 114502731 A CN114502731 A CN 114502731A CN 202080027488 A CN202080027488 A CN 202080027488A CN 114502731 A CN114502731 A CN 114502731A
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polynucleotide
immune cell
cells
seq
cell
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马克·科博尔德
玛吉·李
杰乐米·明舒尔
石丰
水一凡
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Dna20 Co ltd
General Hospital Corp
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Abstract

本发明提供了用于免疫细胞的稳定基因修饰的方法和组合物。基因修饰可用于生成用于治疗或诊断目的的免疫细胞。

Description

基于转座子的免疫细胞的修饰
相关申请的交叉引用
本申请要求2019年2月8日提交的62/803,142的优先权，其全部内容通过引用并入本文用于所有目的。
序列表的引用
本申请涉及在2020年1月28日创建的名称为ST 25_20200128，长度为889,000字节的Txt文件中公开的序列，该文件通过引用并入本文中。
背景技术
免疫细胞的遗传修饰可用于修饰其特性。遗传上可修饰的免疫细胞特性包括免疫细胞识别的分子、免疫细胞内的细胞应答、免疫细胞在某些环境条件下存活的能力、以及免疫细胞产生的蛋白质。免疫细胞的遗传修饰可用于改善它们的疾病靶向应答。通过增强特异性免疫细胞的功能，可以增强免疫应答，例如以实现长期癌症消退。
免疫细胞的稳定遗传修饰可以通过将异源多核苷酸整合到免疫细胞的基因组中来进行。可以以不同的方式将异源DNA导入免疫细胞：通过用裸质粒DNA转染、通过将DNA包装到用于感染免疫细胞的病毒颗粒中、或通过将转座子及其同源转座酶导入免疫细胞中。
非病毒载体系统，包括质粒DNA，通常遭受低效的细胞递送、显著的细胞毒性和有限的转基因表达持续时间，这是由于缺乏基因组插入和导致载体在转染细胞群体中的降解和/或稀释。通过非病毒方法递送的转基因通常形成长的重复阵列(串联体)，其是通过异染色质形成进行转录沉默的靶标。
病毒包装通常对可插入病毒载体的DNA的大小施加限制。一些病毒(例如AAV)作为非复制附加体(non-replicated episome)保留，因此在细胞分裂时稀释。对于将其基因组整合到靶细胞基因组中的病毒，存在关于病毒整合位点的安全性问题。对于所有的病毒递送方法，都存在关于病毒制造的成本和病毒组分的潜在免疫原性的问题。
转座子(transposon)提供了另一种递送系统，它与裸DNA一样制造简单且无免疫原性，但在整合到靶细胞基因组中方面非常有效。转座子包含被转座酶识别的两个末端。转座酶作用于转座子以将其从一个DNA分子上切除并将其整合到另一个中：该过程称为转座(transposition)。转座酶将两个转座子末端之间的DNA随转座子末端一起转座。异源DNA侧翼为一对转座子末端，使得其被转座酶识别并转座，在本文中称为合成转座子(synthetictransposon)。将合成转座子和相应的转座酶导入真核细胞的核中可导致转座子转座到细胞的基因组中。转座子/转座酶基因递送平台具有克服裸DNA和病毒递送限制的潜力。特别地，piggyBac样转座子(piggyBac-like transposon)由于其无限的基因载量(gene cargocapacity)而具有吸引力。
在整合到细胞基因组中的多核苷酸上编码的基因的表达水平取决于该多核苷酸内的序列元件的构型。整合的效率和因此整合到每个基因组中的多核苷酸的拷贝数、以及发生整合的基因组基因座也影响多核苷酸上编码的基因的表达水平。通常可通过将多核苷酸置于转座子中来增加多核苷酸可整合到靶细胞基因组中的效率。
通过piggyBac样转座酶的转座是完全可逆的。转座子在受体DNA分子中的整合靶序列处整合，在此期间靶序列在转座子反向末端重复序列(ITR)的每个末端重复。随后的转座去除转座子并将受体DNA恢复到以前的序列，同时去除靶序列复本和转座子。然而，这不足以从已整合转座子的基因组中去除转座子，因为很可能将转座子从第一整合靶序列中切除，但被整合到基因组中的第二整合靶序列中。另一方面，缺乏整合功能的转座酶可以从第一靶序列上切除转座子，但不能整合到第二靶序列中。因此，整合-缺陷型转座酶可用于逆转转座子的基因组整合。
发明内容
本发明的一个方面是能够稳定修饰免疫细胞基因组的转座子。本发明的一个方面是有利地修饰免疫细胞以增强其功能的基因。本发明的一个方面是用于修饰免疫细胞以增强其功能的方法。
描述了修饰免疫细胞基因组的方法。免疫细胞包括淋巴细胞如T细胞和B细胞以及天然杀伤细胞、T辅助细胞、抗原呈递细胞、树突细胞、嗜中性粒细胞和巨噬细胞。修饰包括增强免疫细胞在某些条件下或在某些环境中存活和/或增殖的能力，改变免疫细胞表面上表达的蛋白质的量或类型，以及改变免疫细胞对接触细胞的蛋白质或小分子的应答。提供了用于影响免疫细胞的基因组修饰的多核苷酸构建体的序列并且是本发明的一个方面。
提高人T细胞功能、稳定性(persistence)和增殖能力是目前肿瘤免疫治疗的瓶颈。高度需要允许T细胞的改善的性能、扩增和遗传操作的技术。控制和扩增T细胞的能力具有许多应用，包括以下：(i)改善T细胞疗法的功能以获得例如与CAR-T组合的更高的功效和/或安全性。(ii)逆转肿瘤浸润性T细胞的T细胞耗竭和/或再刺激诱导的细胞死亡，使T细胞在肿瘤微环境中存活并发挥功能。(iii)改善用于临床前研究(体内)的小鼠中人T细胞的存活。(iv)抗原特异性T细胞的鉴定和体外T细胞受体的克隆。(v)开发可离体维持并且仍执行T细胞的生物学功能(例如细胞杀伤)的T细胞系。
免疫细胞存活增强基因(immune cell survival-enhancing gene)包括抗凋亡基因如生存素、Bcl2、Bcl6、Bcl-XL和编码正常凋亡通路的突变体的基因，该突变体发挥显性负性作用，如Casp3、Casp7、Casp8、Casp9或Casp10.的显性负性突变体。免疫细胞存活增强基因还包括STAT3的激活突变体，包括包含以下突变之一的STAT3突变体：F174S、H410R、S614R、E616K、G618R、Y640F、N647I、E652K、K658Y、K658R、K658N、K658M、K658R、K658H、K658N、D661Y或D661V。免疫细胞存活增强基因还包括CD28的激活突变体，包括包含以下突变之一的CD28突变体：D124E、D124V、T195I或T195P。免疫细胞存活增强基因还包括RhoA的激活突变体，包括包含以下突变之一的RhoA突变体：G17V或K18N。免疫细胞存活增强基因还包括磷脂酶Cγ的激活突变体，包括包含以下突变之一的磷脂酶Cγ突变体：S345F、S520F或R707Q。免疫细胞存活增强基因还包括STAT5B的激活突变体，包括包含以下突变之一的STAT5B突变体：N642H、T648S、S652Y、Y665F或P267A。免疫细胞存活增强基因还包括CCND1的激活突变体，包括包含以下突变之一的CCND1突变体：E36G、E36Q、E36K、A39S、S41L、S41P、S41T、V42E、V42A、V42L、V42M、Y44S、Y44D、Y44C、Y44H、K46T、K46R、K46N、K46E、C47G、C47R、C47S、C47W、P199R、P199S、P199L、S201F、T285I、T285A、P286L、P286H、P286S、P286T或P286A。
免疫细胞存活增强基因还包括增强型信号传导受体(ESR)，其中ESR包括衍生自通常向免疫细胞传递抑制信号的受体的胞外结构域的序列、衍生自向免疫细胞传递刺激信号的受体的胞内结构域和跨膜结构域的序列。示例性胞外结构域包括选自TNFRSF3(LTRβ)、TNFRSF6(Fas)、TNFRSF8(CD30)、TNFRSF10A(DR4)、TNFRSF10B(DR5)、TNFRSF19(TROY)、TNFRSF21(DR6)和CTLA4的人蛋白，例如SEQ ID NO：322-340。示例性胞内结构域包括选自以下的人蛋白：TNFRSF4(OX40)、TNFRSF5(CD40)、TNFRSF7(CD27)、TNFRSF9(4-1BB)、TNFRSF11A(RANK)、TNFRSF13B(TACI)、TNFRSF13C(BAFF-R)、TNFRSF14(HVEM)、TNFRSF17(CD269)、TNFRSF18(GITR)、CD28、CD28H(TMIGD2)、诱导型T细胞共刺激因子(ICOS/CD278)、DNAX辅助分子1(DNAM-1/CD226)、信号转导淋巴细胞激活分子(SLAM/CD150)、T细胞免疫球蛋白和粘蛋白结构域(TIM-1/HAVcr-1)、干扰素受体α链(IFNAR1)、干扰素受体β链(IFNAR2)、白细胞介素2受体β亚基(IL2RB)、白细胞介素2受体γ亚基(IL2RG)、肿瘤坏死因子超家族14(TNFSF14/LIGHT)、自然杀伤细胞2族成员D(NKG2D/CD314)和CD40配体(CD40L)，例如SEQ IDNO：341-364。示例性的跨膜结构域包括选自365-396的人蛋白。示例性增强型信号传导受体包括这样的序列，其包含选自SEQ ID NO：274-318的序列。
优选地，使用转座子载体向免疫细胞提供免疫细胞存活增强基因。转座子通过相应的转座酶有效地整合到免疫细胞基因组中。几种不同种类的转座子可用于将基因整合到免疫细胞的基因组中。PiggyBac样转座子，例如包含含有SEQ ID NO：18和19的ITR序列的菜蛾piggyBac转座子、或包含含有SEQ ID NO：20和21的ITR序列的piggyBat转座子、或包含含有SEQ ID NO：6和7的ITR序列的爪蟾piggyBac样转座子、或包含含有SEQ ID NO：14和15的ITR序列的家蚕piggyBac样转座子均可被相应的转座酶转座到免疫细胞基因组中。而且，Mariner型转座子如睡美人，其包含含有SEQ ID NO：26和27的ITR，可以被相应的转座酶转座到免疫细胞基因组中。而且，hAT型转座子如TcBuster，其包含含有SEQ ID NO：399和400的ITR，可以被相应的转座酶转座到免疫细胞基因组中。任何这些转座子可用于将存活增强基因整合到免疫细胞基因组中。可以用相应的转座酶将转座子导入免疫细胞。转座酶可以作为蛋白质提供，或者作为编码转座酶的核酸提供，如mRNA分子或DNA分子,其具有与可在免疫细胞中表达的启动子可操作地连接的编码转座酶的序列。也可以将其他基因导入免疫细胞以修饰其功能。例如，可以导入编码允许免疫细胞与靶细胞表面上的抗原结合的受体的基因。还可以导入可用于杀伤免疫细胞的基因。使用转座子将基因组合递送到免疫细胞的益处是转座酶通常将转座子ITR之间的所有DNA整合到免疫细胞的基因组中。因此可以同时导入多个基因。
将免疫细胞存活基因整合到免疫细胞前体细胞如干细胞的基因组中，然后使免疫细胞前体细胞分化为免疫细胞是可行的。明确地考虑了这样的操作。为了增强免疫细胞的存活，应将存活增强基因可操作地连接至启动子，使得存活增强基因可在免疫细胞中表达。示例性启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQ ID NO 94-154的启动子。
本发明的一个方面是修饰的人免疫细胞。此外，已经被修饰以增强其存活或增殖的动物免疫细胞作为实验模型和作为动物治疗剂也是有价值的。本发明的一个方面是来自包括灵长类、啮齿类、猫科动物、犬类和马类等哺乳动物的修饰的免疫细胞。
附图说明
图1.用爪蟾或家蚕piggyBac样基因转移系统转染的Jurkat人T细胞系的FACS分析。如6.1.1节所述，用转座酶和包含CD19基因的相应转座子转染人Jurkat细胞。70天后，使用FACS分选仪选择表达CD19的细胞并在培养物中再生长85天。然后对细胞进行CD19染色并在FACS上进行分析。图A：分析最初用如SEQ ID NO：223所示序列的转座子转染的细胞的CD19(y轴)和GFP(x轴)。图B：分析最初用如SEQ ID NO：224所示序列的转座子转染的细胞的CD19(y轴)和RFP(x轴)。
图2.用编码突变STAT3的基因转染的原代T细胞的FACS分析。用转座酶和相应的转座子共转染人原代T细胞，该转座子包含编码STAT3突变形式(STAT3-Y640F)的基因和编码绿色荧光蛋白(GFP)的基因。图A：将细胞培养不同的时间(在顶部显示)，之后取样，用荧光标记的抗CD8抗体标记，并使用荧光激活细胞分选仪分析。在T细胞表面上表达的CD8显示在y轴上，GFP(其指示了包含STAT3Y640F基因的转座子的存在)显示在x轴上。图B：由图A中显示的数据计算显示GFP荧光的CD8+细胞分数(fraction)。
图3.转染的原代T细胞和来自JY B细胞系的细胞的混合物的FACS分析。如6.2.1.3节中所述，用转座酶和3个相应转座子其中之一共转染人原代T细胞，该转座子包含编码序列如SEQ ID NO：229所示的嵌合抗原受体的基因和GFP报告基因。一个转座子不包含其他基因(图A和D)，一个转座子还包含编码生存素的基因(图B和E)，以及一个转座子还包含编码CD28-D124E-T195P的基因(图C和F)。将细胞培养约5周，此时约10％的T细胞表达GFP。此时将200,000个T细胞(＝20,000个表达GFP的T细胞)与200,000个JY转化的B细胞系混合。混合后3天(图A、C和E)或7天(图B、D和F)后，用荧光标记的抗CD8和抗CD19抗体标记细胞，并使用荧光激活细胞分选仪进行分析。在T细胞表面上表达的CD8显示在y轴上，在JY细胞表面上表达的CD19显示在x轴上。
图4.用编码Bcl-2和Bcl-6的基因转染的原代T细胞的FACS分析。用转座酶和相应的转座子共转染人原代T细胞，该转座子包含编码Bcl2和BBcl6的基因和编码绿色荧光蛋白(GFP)的基因。图A：将细胞培养不同的时间(在顶部显示)，之后取样，用荧光标记的抗CD8抗体标记，并使用荧光激活细胞分选仪分析。在T细胞表面上表达的CD8显示在y轴上，GFP(其指示了包含Bcl2-2A-Bcl6基因的转座子的存在)显示在x轴上。图B：由图A中显示的数据计算显示GFP荧光的CD8+细胞分数。
图5.来自用编码Bcl-XL的基因转染的3个供体的原代T细胞的FACS分析。用转座酶和相应的转座子共转染人原代T细胞，该转座子包含编码Bcl-XL的基因和编码绿色荧光蛋白(GFP)的基因。细胞在培养物中生长240天，用抗人CD8的荧光标记抗体染色并在流式细胞仪上分析。图A：供体81；图A：供体82；图A：供体84。在T细胞表面上表达的CD8显示在y轴上，GFP(其指示包含Bcl-XL基因的转座子的存在)显示在x轴上。
具体实施方式
5.1定义
除非上下文另外明确指出，否则单数形式“一种(a、an)”和“该(the)”的使用包括复数指代。因此，例如，提及“多核苷酸(a polynucleotide)”包括多个多核苷酸，提及“底物(a substrate)”包括多个这样的底物，提及“变体(a variant)”包括多个变体等。
除非上下文另外明确指出，否则术语例如“连接的(connected)”、“附接的(attached)”、“连结的(linked)”和“缀合的(conjugated)”在本文中可互换使用，并且涵盖直接以及间接的连接、附接、连结或缀合。在列举数值范围的情况下，应当理解，也具体公开了在该范围的列举的上限和下限之间的每个中间整数值及其每个分数，以及在这些数值之间的每个子范围。任何范围的上限和下限可以独立地包括在该范围内或从该范围排除，并且包括任一、两个或两个极限的每个范围也包括在本发明内。当所讨论的值具有固有极限时，例如当组分可以0-100％的浓度存在时，或当水溶液的pH可以为1-14时，这些固有极限被具体公开。在明确列举数值的情况下，应当理解的是，与所列举的数值大约相同数量或量的值也在本发明的范围内。在公开组合的情况下，该组合的要素的每个子组合也被具体公开并且在本发明的范围内。相反，在单独公开不同的要素或要素组的情况下，也公开了其组合。在本发明的任何要素被公开为具有多个替代方案的情况下，还由此公开了本发明的实施例，其中每个替代方案单独地或以与其他替代方案的任何组合被排除；本发明的一个以上的要素可以具有这样的排除，并且具有这样的排除的要素的所有组合由此被公开。
除非本文另外定义，否则本文使用的所有技术和科学术语具有与本发明所属领域的普通技术人员通常理解的相同含义。Singleton等人，Dictionary of Microbiology andMolecular Biology，2nd Ed.(微生物学和分子生物学词典，第二版)，John Wiley andSons，New York(约翰·威利父子出版公司，纽约)(1994)，以及Hale&Marham,The HarperCollins Dictionary of Biology，Harper Perennial(哈珀·柯林斯生物学词典)，NY，1991，为技术人员提供了本发明中使用的许多术语的通用词典。尽管在本发明的实践或测试中可以使用与本文所述的那些类似或等同的任何方法和材料，但描述了优选的方法和材料。除非另有说明，核酸以5'至3'方向从左到右书写；氨基酸序列分别以氨基至羧基的方向从左到右书写。下面紧接着定义的术语通过参考说明书整体而更全面地定义。
多核苷酸的“构型(configuration)”是指多核苷酸内的功能序列元件、以及这些元件的顺序和方向。
术语“相应的转座子(corresponding transposon)”和“相应的转座酶(corresponding transposase)”用于表示转座酶和转座子之间的活性关系。转座酶转座其相应的转座子。
术语“反向可选择性标记(counter-selectable marker)”是指赋予宿主细胞选择性缺点的多核苷酸序列。反向可选择性标记的实例包括sacB，rpsL、tetAR、pheS、thyA、gata-1、ccdB、kid和barnase(Bernard，1995，Journal/Gene，162：159-160；Bernard等人，1994.Journal/Gene，148：71-74；Gabant等人,1997，Journal/Biotechniques，23：938-941；Gababt等人,1998，Journal/Gene，207：87-92；Gababt等人,2000，Journal/Biotechniques，28：784-788；Galvao和de Lorenzo，2005，Journal/Appl Environ Microbiol，71：883-892；Hartzog等人,2005，Journal/Yeat，22:789-798；Knipfer等人,1997，Journal/Plasmid，37：129-140；Reyrat等人,1998，Journal/Infect Immun，66：4011-4017；Soderholm等人,2001，Journal/Biotechniques，31：306-310，312；Tamura等人,2005，Journal/Appl EnvironMicrobiol，71：587-590；Yazynin等人,1999，Journal/FEBS Lett，452：351-354)。反向可选择性标记通常在特定背景中赋予它们选择性的缺点。例如，它们可以赋予对可以加入宿主细胞环境中的化合物的敏感性，或者它们可以杀伤具有一种基因型的宿主但不杀伤具有不同基因型的宿主。不赋予携带反向可选择性标记的细胞选择性缺点的条件被描述为“允许的(permissive)”。确实赋予携带反向可选择性标记的细胞选择性缺点的条件被描述为“限制性的(restrictive)”。
术语“偶联元件(coupling element)”或“翻译偶联元件(ranslational couplingelement)”是指允许第一多肽的表达与第二多肽的表达连接的DNA序列。内部核糖体进入位点元件(IRES元件)和顺式作用水解酶元件(CHYSEL元件)是偶联元件的实例。
术语“DNA序列”、“RNA序列”或“多核苷酸序列”是指连续的核酸序列。该序列可以是长度为2至20个核苷酸的寡核苷酸至数千或数十万碱基对的全长基因组序列。
术语“增强型信号传导受体(Enhanced Signaling Receptor)”(或“ESR”)是指其中向免疫细胞传递抑制信号的受体的胞外/配体结合结构域与传递刺激信号的受体的胞内结构域融合的蛋白质。
术语“表达构建体(expression construct)”是指设计用于转录RNA的任何多核苷酸。例如，包含至少一个启动子(与或可能与下游基因可操作地连接)、编码区或多核苷酸序列的构建体(例如，编码多肽或蛋白质的cDNA或基因组DNA片段；或RNA效应分子，例如反义RNA、形成三链体的RNA、核酶、人工选择的高亲和力RNA配体(适配体)、双链RNA，例如包含茎-环或发夹dsRNA的RNA分子、或双指或多指dsRNA或微小RNA、或任何RNA)。“表达载体(expression vector)”是包含可与第二多核苷酸可操作地连接的启动子的多核苷酸。将表达构建体转染或转化到受体细胞中使得细胞表达由表达构建体编码的RNA效应分子、多肽或蛋白质。表达构建体可以是经基因工程改造的质粒、病毒、重组病毒或衍生自例如噬菌体、腺病毒、腺相关病毒、逆转录病毒、慢病毒、痘病毒或疱疹病毒的人工染色体。这样的表达载体可以包括来自细菌、病毒或噬菌体的序列。这类载体包括染色体、附加体(episomal)和病毒衍生的载体，例如，衍生自细菌质粒、噬菌体、酵母附加体、酵母染色体元件和病毒的载体，以及衍生自其组合的载体，例如衍生自质粒和噬菌体遗传元件、粘粒和噬菌粒的载体。表达构建体可在活细胞中复制或可合成制备。出于本申请的目的，术语“表达构建体”、“表达载体”、“载体”和“质粒”可互换使用，以在一般的说明性意义上展示本发明的应用，并且不旨在将本发明限于特定类型的表达构建体。
术语“表达多肽(expression polypeptide)”是指由表达构建体上的基因编码的多肽。
术语“表达系统(expression system)”是指用于产生由多核苷酸编码的一种或多种基因产物的任何体内或体外生物系统。
“基因转移系统(gene transfer system)”包括载体或基因转移载体、或包含克隆到载体中的待转移基因的多核苷酸(“基因转移多核苷酸”或“基因转移构建体”)。基因转移系统还可以包括其他特征以促进基因转移的过程。例如，基因转移系统可以包含载体和用于使第一多核苷酸进入细胞的脂质或病毒包装混合物，或者它可以包含包括转座子的多核苷酸和编码相应转座酶以增强转座子的生产性基因组整合的第二多核苷酸序列。基因转移系统的转座酶和转座子可以位于相同的核酸分子上或位于不同的核酸分子上。基因转移系统的转座酶可以作为多核苷酸或多肽提供。
两个元件如果不是天然相关的，则彼此是“异源的”。例如，编码与异源启动子连接的蛋白的核酸序列是指不同于天然驱动蛋白质表达的启动子。侧翼为转座子末端或ITR的异源核酸是指天然情况下侧翼不是那些转座子末端或ITR的异源核酸，例如编码不同于转座酶的多肽的核酸，包括抗体重链或轻链。如果核酸不是天然存在于细胞，或者如果天然存在于细胞中但是位于与所述定位不同的定位(例如，附加体或不同的基因组定位)，则核酸对于细胞是异源的。
术语“宿主”是指可以是核酸受体的任何原核或真核生物。本文所用术语“宿主”包括可进行基因工程改造的原核或真核生物。这些宿主的例子参见Maniatis等人，MolecularCloning.A Laboratory Manual(分子克隆实验指南)，Cold Spring Harbor Laboratory，Cold Spring Harbor，N.Y.(冷泉港实验室，冷泉港，纽约)(1982)。如本文所用，术语“宿主”、“宿主细胞”、“宿主系统”和“表达宿主”可互换使用。
“IRES”或“内部核糖体进入位点”是指独立于帽结构直接促进核糖体结合的特异性序列。
“分离的(isolated)”多肽或多核苷酸是指已经从其天然环境中移除、使用重组技术产生或化学或酶促合成的多肽或多核苷酸。本发明的多肽或多核苷酸可以是纯化的，即基本上不含任何其他多肽或多核苷酸和相关的细胞产物或其他杂质。
术语“核苷(nucleoside)”和“核苷酸(nucleotide)”包括不仅含有已知的嘌呤和嘧啶碱基，还含有其他经修饰的杂环碱基的那些部分。这些修饰包括甲基化嘌呤或嘧啶、酰化嘌呤或嘧啶或其他杂环。修饰的核苷或核苷酸还可以包括在糖部分上的修饰，例如，其中一个或多个羟基被卤素、脂肪族基团替代，或官能化为醚、胺等。术语“核苷酸单元(nucleotidic unit)”旨在涵盖核苷和核苷酸。
“开放阅读框”或“ORF”是指当被翻译成氨基酸时不含终止密码子的多核苷酸的一部分。遗传密码以3个碱基对组成的组读取DNA序列，这意味着双链DNA分子可以以6个可能的阅读框中的任何一个阅读—3个正向读框，3个反向读框。ORF通常还包括起始密码子，翻译可在该起始密码子处开始。
术语“可操作地连接”是指两个序列之间的功能性连接，使得一个序列修饰另一个序列的行为。例如，如果第一多核苷酸影响第二多核苷酸的转录和/或翻译，则包含核酸表达控制序列(例如启动子、IRES序列、增强子或转录因子结合位点阵列)的第一多核苷酸与第二多核苷酸可操作地连接。类似地，如果第一氨基酸序列导致第二氨基酸序列被分泌或定位至亚细胞定位，则包含分泌信号或亚细胞定位信号的第一氨基酸序列与第二氨基酸序列可操作地连接。
术语“突出端(overhang)”或“DNA突出端(DNAoverhang)”是指双链DNA分子末端的单链部分。互补突出端是彼此碱基配对的突出端。
“piggyBac样转座酶(piggyBac-like transposase)”是指使用TBLASTN算法鉴定的与来自粉纹夜蛾(Trichoplusia ni)的piggyBac转座酶(SEQ IDNO：30)具有至少20％序列同一性的转座酶，更全面地描述于Sakar，A.等人，(2003).Mol.Gen.Genomics 270：173-180.“Molecular evolutionary analysis of the widespread piggyBac transposonfamily and related'domesticated'species(广泛分布的piggyBac转座子家族和相关的‘驯化’物种的分子进化分析)”，并且进一步的特征在于DDE样DDD基序，在最大比对处在对应于粉纹夜蛾piggyBac转座酶D268、D346和D447的位置具有天冬氨酸残基。piggyBac样转座酶的特征还在于它们能够以高频率精确地切除它们的转座子。“piggyBac样转座子(piggyBac-like transposon)”是指具有转座子末端的转座子，该转座子末端与编码piggyBac样转座酶的天然存在的转座子的转座子末端相同或具有至少80％、优选至少90％、95％、96％、97％、98％或99％同一性。piggyBac样转座子在每端包括约12-16个碱基的反向末端重复(ITR)序列。这些重复在两端可以相同，或者在两端的重复在两个ITR中的1或2或3或4个位置可以不同。转座子的两侧各有一个4碱基序列，对应于转座子整合中重复的整合靶序列(靶位点重复或靶序列重复或TSD)。
术语“多核苷酸”、“寡核苷酸”、“核酸”和“核酸分子”以及“基因”可互换使用，是指任何长度的核苷酸的聚合形式，并且可以包括核糖核苷酸、脱氧核糖核苷酸、其类似物或其混合物。该术语仅指分子的一级结构。因此，该术语包括三链、双链和单链脱氧核糖核酸(“DNA”)，以及三链、双链和单链核糖核酸(“RNA”)。它还包括修饰的(例如通过烷基化、和/或通过加帽)和未修饰形式的多核苷酸。更具体地，术语“多核苷酸”、“寡核苷酸”、“核酸”和“核酸分子”包括多脱氧核糖核苷酸(含有2-脱氧-D-核糖)、多核糖核苷酸(含有D-核糖)，包括tRNA、rRNA、hRNA、siRNA和mRNA，无论是剪接的还是未剪接的、以及任何其他类型的核酸，该核酸是嘌呤或嘧啶碱的N-或C-糖苷以及其他包含非核苷酸主链的聚合物，例如，聚酰胺(例如，核酸肽类(PNA))以及聚吗啉代(可从俄勒冈州科瓦利斯市的Anti-Virals公司以Neugene名称商购)聚合物以及其他合成的序列特异性核酸聚合物，其条件是这些聚合物包含处于一种构型的核碱基，该构型允许碱基配对以及碱基堆积，例如在DNA以及RNA中发现的一样。术语“多核苷酸”、“寡核苷酸”、“核酸”和“核酸分子”之间在长度上没有有意的区别，并且这些术语在本文中可互换使用。这些术语仅指分子的一级结构。因此，这些术语包括例如3'-脱氧-2',5'DNA、寡脱氧核糖核苷酸N3'P5'氨基磷酸酯、2'O-烷基取代的RNA、双链和单链DNA以及双链和单链RNA及其杂交体，包括例如，DNA和RNA之间或PNA和DNA或RNA之间的杂交体，还包括已知类型的修饰，例如标记、烷基化、“加帽”、用类似物取代一个或多个核苷酸、核苷酸内修饰，例如，那些具有不带电荷的键(例如，甲基膦酸酯、磷酸三酯、氨基磷酸酯、氨基甲酸酯等)、带负电荷的键(例如，硫代磷酸酯、二硫代磷酸酯等)和带正电荷的键(例如，氨基烷基氨基磷酸酯、氨基烷基磷酸三酯)的修饰；那些含有悬垂部分(pendantmoieties)的修饰例如，蛋白质(包括酶(例如，核酸酶)、毒素、抗体、信号肽、聚L-赖氨酸等)；那些具有嵌入剂(例如，吖啶、补骨脂素等)的修饰；那些含有(例如金属，放射性金属、硼、氧化金属等的)螯合物的修饰；那些含有烷化剂的修饰；那些具有修饰键(例如α端基异构核酸等)的修饰；以及未修饰形式的多核苷酸或寡核苷酸。
“启动子”是指足以指导可操作地连接的核酸分子转录的核酸序列。启动子可与其他转录控制元件(例如增强子)一起使用，其他转录控制元件足以使启动子依赖性基因表达以细胞类型特异性、组织特异性或时间特异性方式可控，或可由外部信号或试剂诱导；这些元件可以位于基因的3'区内或内含子内。期望地，启动子可操作地连接到核酸序列，例如cDNA或基因序列、或效应子RNA编码序列，以使核酸序列能够表达的方式或者在表达盒中提供启动子，其中待转录的选定核酸序列可以方便地插入到表达盒中。
术语“选择性标记(selectable marker)”是指允许人们通常在特定条件下选择或针对含有它的分子或细胞的多核苷酸片段。这些标记可以编码活性，例如但不限于RNA、肽或蛋白质的产生，或者可以提供RNA、肽、蛋白质、无机和有机化合物或组合物的结合位点。选择性标记的实例包括但不限于：(1)编码对其他有毒化合物(例如抗生素)提供抗性的产物的DNA片段；(2)编码受体细胞中缺乏的产物(例如tRNA基因、营养缺陷标记)的DNA片段；(3)编码抑制基因产物活性的产物的DNA片段；(4)编码易于鉴定的产物(例如，表型标记例如β-半乳糖苷酶、绿色荧光蛋白(GFP)和细胞表面蛋白)的DNA片段；(5)结合对细胞存活和/或功能有害的产物的DNA片段；(6)抑制上述No.1-5中描述的任何DNA片段(例如，反义寡核苷酸)活性的DNA片段；(7)结合修饰底物的产物(例如限制性内切核酸酶)的DNA片段；(8)可用于分离所需分子(例如特异性蛋白结合位点)的DNA片段；(9)编码特异性核苷酸序列的DNA片段，该核苷酸序列另外可以是非功能性的(例如，用于分子亚群的PCR扩增)；和/或(10)在不存在时直接或间接赋予对特定化合物敏感性的DNA片段。
序列同一性可以通过使用算法比对序列来确定，例如在Wisconsin GeneticsSoftware Package Release 7.0，Genetics Computer Group，575Science Dr.，Madison，Wis.中使用默认缺口参数的BESTFIT、FASTA和TFASTA，或通过检查，以及最佳比对(即，在比较窗口中产生最高百分比的序列相似性)。序列同一性百分比通过比较比较窗口上的两个最佳比对序列、确定两个序列中相同残基出现的位置数以产生匹配位置数、将匹配位置数除以不计算比较窗口中的间隙的匹配和不匹配位置的总数(即窗口大小)、将结果乘以100以得到序列同一性百分比。除非另有说明，两个序列之间的比较窗口由两个序列中较短序列的全长限定。
睡美人转座酶是一种Mariner型转座酶，其序列与SEQ ID NO：28具有至少90％、95％、99％或100％同一性，能够将具有左端序列SEQ ID NO：24和右端序列SEQ ID NO：25的转座子转座到宿主细胞的基因组中。睡美人转座子包含与SEQ ID NO：26具有至少90％、95％、99％或100％同一性的左ITR以及与SEQ ID NO：27具有90％同一性的右ITR。睡美人转座子可以包含与SEQ ID NO：24具有至少90％、95％、99％或100％同一性的转座子末端(包括ITR)以及与SEQ ID NO：25具有至少90％、95％、99％或100％同一性的右转座子末端(包括ITR)，并且其可以通过如SEQ ID NO：28所示的睡美人转座酶转座到宿主细胞的基因组中。
“靶核酸”是一种将转座子插入其中的核酸。这样的靶标可以是染色体、附加体或载体的一部分。
转座酶的“整合靶序列”或“靶序列”或“靶位点”是靶DNA分子中可以通过转座酶插入转座子的位点或序列。来自粉纹夜蛾(Trichoplusia ni)的piggyBac转座酶将其转座子主要插入靶序列5'-TTAA-3’中。piggyBac样转座酶使用剪切和粘贴机制将它们的转座子转座，这导致它们的4个碱基对靶序列在插入DNA分子时复制。因此，靶序列存在于整合的piggyBac样转座子的每一侧。
术语“翻译”是指通过核糖体“读取”多核苷酸序列来合成多肽的过程。
“转座酶”是一种多肽，其催化从供体多核苷酸(例如载体)中切除相应的转座子，并且(假设转座酶不是整合缺陷的)随后将转座子整合到靶核酸中。转座酶可以是piggyBac样转座酶或mariner转座酶如睡美人。
本文所用术语“转座”是指转座酶在从一个多核苷酸上切除转座子然后将其整合到同一多核苷酸的不同位点或第二多核苷酸中的作用。
术语“转座子”是指可通过相应的反式作用转座酶的作用从第一多核苷酸(例如，载体)切除并整合到同一多核苷酸中的第二位置或第二多核苷酸(例如，细胞的基因组或染色体外DNA)中的多核苷酸。转座子包含第一转座子末端和第二转座子末端，它们是由转座酶识别和转座的多核苷酸序列。转座子通常还包含位于两个转座子末端之间的第一多核苷酸序列，使得第一多核苷酸序列在转座酶的作用下与两个转座子末端一起转座。天然转座子通常包含编码作用于转座子的转座酶的DNA。本发明的转座子是包含异源多核苷酸序列的“合成转座子”，该序列凭借其在两个转座子末端之间的并置而可转座。转座子可以是piggyBac样转座子或marine转座子如睡美人。
术语“转座子末端”是指足以被相应转座酶识别和转座的顺式作用核苷酸序列。piggyBac样转座子的转座子末端包含完美或不完美的重复，使得两个转座子末端中的相应重复是彼此的反向互补。这些称为反向末端重复(ITR)或末端反向重复(TIR)。转座子末端可以包括或不包括邻近ITR的促进或增强转座的额外序列。
术语“载体”或“DNA载体”或“基因转移载体”是指用于对另一多核苷酸执行“携带”功能的多核苷酸。例如，通常使用载体以允许多核苷酸在活细胞内增殖、或允许多核苷酸被包装以递送到细胞中、或允许多核苷酸整合到细胞的基因组DNA中。载体还可以包含另外的功能元件，例如它可以包含转座子。
免疫细胞可以指免疫系统的任何细胞，包括适应性和先天免疫系统的细胞，并且包括骨髓或淋巴来源的细胞。免疫细胞的实例包括白细胞、淋巴细胞、巨噬细胞、嗜中性粒细胞、树突细胞、淋巴样细胞、肥大细胞、嗜酸性粒细胞、嗜碱性粒细胞和天然杀伤细胞。淋巴细胞包括B淋巴细胞和T淋巴细胞。T淋巴细胞包括杀伤T细胞、辅助T细胞和γδT细胞。免疫细胞可以是从受试者分离的原代细胞，或者可以是进一步培养的结果，包括以细胞系的形式。除了本文所述的之外，免疫细胞可以是基因工程的受试者，例如CART受体的表达。
本公开涉及几种蛋白质，其提供了代表该蛋白质的野生型人序列的示例性SEQ IDNO.。除非从上下文中另有说明，否则提及蛋白质应理解为包括所例示的SEQ ID NO.以及与其具有至少90％、95％或99％同一性的其等位基因变体、物种变体和诱导变体。等位基因和物种变体的实例可以在SwissProt和其他数据库中找到。可以修饰蛋白质的任何此类序列以包括本文所述的一个或多个激活突变，以赋予表达如本文进一步所述的蛋白质的免疫细胞增强的存活。
有时以XnY的形式提及突变，其中X为野生型氨基酸，n为野生型序列中X的氨基酸位置，并且Y为置换氨基酸。如果突变发生在与野生型序列具有不同数目氨基酸的序列中，则当各序列最大比对时，它存在于序列中与野生型序列中位置n比对的位置处。
如果核酸被认为编码特定蛋白的激活突变体，则意味着核酸编码包括激活突变的蛋白。
凋亡抑制因子(apoptosis inhibitor)是一种干扰程序性细胞死亡(凋亡)过程的物质。细胞凋亡是高度调控的过程，其中细胞死亡是通过激活细胞内半胱天冬酶蛋白酶诱导的。凋亡抑制因子包括其天然功能是对抗细胞凋亡的蛋白，以及其天然功能是参与细胞凋亡但包含干扰细胞凋亡的突变的蛋白。
凋亡测定法检测和定量与程序性细胞死亡相关的细胞事件，包括半胱天冬酶(caspase)激活、磷脂酰丝氨酸(PS)的细胞表面暴露和DNA断裂。起始子和效应子半胱天冬酶是检测细胞凋亡的特别好的靶标。半胱天冬酶活性测定使用由天冬氨酸特异性半胱氨酸蛋白酶裂解的肽底物，或结合到活细胞中的激活的半胱天冬酶的类似底物(McStay等人，2014Cold Spring Harbor Protocols，Measuring Apoptosis：Caspase Inhibitors andActivity assays(2014冷泉港协议，测量细胞凋亡：半胱天冬酶抑制因子和活性测定)；Niles等人，2008，Methods Mol Biol.,414:137-50)。测量细胞凋亡抑制的示例性测定是使用本文第[00174]段中描述的荧光素酶的生物发光测定许多使用荧光或发光读数的半胱天冬酶测定试剂盒是可商购的，例如来自Promega的caspase-
Figure GDA0003583385470000171
测定使用发光的半胱天冬酶-8四肽底物(Z-LETD-氨基荧光素)、半胱天冬酶-9四肽底物(Z-LEHD-氨基荧光素)、半胱天冬酶-3/7底物(Z-DEVD-氨基荧光素)、半胱天冬酶-6底物(Z-VEID-氨基荧光素)或半胱天冬酶-2底物(Z-VDVAD-氨基荧光素)以及在专用缓冲液中稳定的荧光素酶。在缺乏活性半胱天冬酶或半胱天冬酶抑制的情况下，半胱天冬酶底物不作为荧光素酶的底物，因此不产生光。在底物被各自的胱天蛋白酶裂解时，氨基荧光素被释放并可在发光反应中有助于光的产生。产生的发光信号与样品中存在的半胱天冬酶活性的量成正比。使用荧光底物N-乙酰Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素或Ac-DEVDAMC检测半胱天冬酶-3的半胱天冬酶活性测定试剂盒的实例是来自Cell Signaling Technology(CST)的半胱天冬酶-3活性测定试剂盒。激活的半胱天冬酶-3在DEVD和AMC之间切割这种底物，产生高荧光AMC，可以使用荧光读取器检测到，激发波长为380nm，发射波长为420-460nm。底物的裂解仅发生在凋亡细胞的裂解物中；因此，产生的AMC的量与样品中凋亡细胞的数量成比例。
5.2用于在免疫细胞中表达的遗传元件
5.2.1转座子元件
如果异源多核苷酸整合到宿主细胞的基因组中，则可以提高免疫细胞中异源多核苷酸基因表达的一致性通过使多核苷酸经历确保基因组DNA复制和分裂的相同机制，将多核苷酸整合到宿主细胞的基因组中通常也使其可稳定遗传。这种稳定的遗传性对于在长的生长周期内获得良好和一致的表达是理想的。对于免疫细胞的稳定修饰，特别是对于治疗应用，修饰的稳定性和表达水平的一致性是很重要的。
如果异源多核苷酸是转座子的一部分，则它们可以更有效地整合到靶基因组中，例如使得它们可以通过转座酶整合。转座子的特别益处是转座子ITR之间的完整多核苷酸被整合。这与随机整合相反，在随机整合中，导入真核细胞的多核苷酸通常在细胞中随机片段化，并且只有部分多核苷酸通常以低频率并入靶基因组。掺入piggyBac样转座子的异源多核苷酸可以整合到免疫细胞，以及肝细胞、神经细胞、肌细胞、血细胞、胚胎干细胞、体干细胞、造血细胞、胚胎、受精卵和精子细胞(其中一些是开放的以在体外环境中操作)中。优选的细胞还可以是多能细胞(其后代可以分化成几种限制性细胞类型的细胞，例如造血干细胞或其他干细胞)或全能细胞(即，其后代可以变成生物体中的任何细胞类型的细胞，例如胚胎干细胞)。
优选的基因转移系统包括与使转座子转座的相应转座酶蛋白或编码相应转座酶蛋白并可在靶细胞中表达的核酸组合的转座子。由于几个原因，与慢病毒载体相比，piggyBac样转座子作为用于本文所述应用的基因转移系统是有利的。如果慢病毒超过一定大小，则不能有效地被包装，并且它们的大量的DNA已经被病毒合成、装配和包装所需的序列占据。由于启动子沉默，通过慢病毒载体整合的基因可显示高度可变的表达(Antoniou等人，2013.Hum Gene Ther 24，363-374.“Optimizing retroviral gene expression foreffective therapies(优化逆转录病毒基因表达以用于有效治疗)”)：可以通过增加拷贝数或通过将绝缘子掺入整合多核苷酸来降低沉默(Emery，2011.Hum Gene Ther 22，761-774.“The use of chromatin insulators to improve the expression and safety ofintegrating gene transfer vectors(使用染色质绝缘子提高整合基因转移载体的表达和安全性)”)。由于大小限制以及由于包括这些序列对病毒包装和滴度的影响，在慢病毒构建体中包括绝缘子可能具有挑战性。相比之下，piggyBac样转座子通过其相应的转座酶有效整合到靶基因组中不受增加转座子大小的影响。因此，可以将多个用于修饰免疫细胞性质的基因与侧翼绝缘子一起整合到单个转座子中，而不会损害相应转座酶将转座子整合到免疫细胞基因组中的能力。当修饰待置于人体内的细胞的基因组时，安全性也是重要的考虑因素。当对免疫细胞如T细胞进行修饰以增强其杀伤肿瘤细胞的能力并改善其存活和增殖的能力时，因此能够将提供杀伤经修饰的免疫细胞的方法的基因也掺入细胞的基因组中是有用的。这种“杀伤开关(kill switch)”的实例包括由现有治疗剂有效识别的抗原的表达(例如表面表达的抗原，如通常仅在B细胞上发现并且由药物利妥昔单抗识别和治疗的CD20，或通常仅在B细胞上发现并且由药物blinoumomab识别和治疗的CD19)和诱导型半胱天冬酶9自杀开关(Straathof等人，2005.Blood 105，4247-4254.“用于T细胞疗法的诱导型半胱天冬酶9安全开关”)。为了使杀伤开关有用，它们必须存在于每个修饰细胞的基因组中。在携带嵌合抗原受体基因和编码CD19选择性标记的序列以及作为杀伤开关的诱导型半胱天冬酶9的慢病毒载体中进行这种尝试的实例描述于Budde 等人(PLoS One 8(12)：e82742.doi：10.1371/journal.pone.0082742.eCollection 2013.“组合CD20嵌合抗原受体和诱导型半胱天冬酶9自杀开关以改善T细胞过继性免疫治疗淋巴瘤的功效和安全性”)。为了将杀伤开关与嵌合抗原受体基因组合，作者必须使用病毒CHYSL/2A序列将包含嵌合抗原受体的多肽与诱导型半胱天冬酶9分离，并且它们必须截短CD19基因。该货物(cargo)和用于表达的调控元件基本上占据了慢病毒载体的全部容量，没有留下用于添加绝缘子或用于其他基因(例如用于增强T细胞的存活或增殖或功能的那些基因)的额外空间。因此，包含piggyBac样转座子及其相应转座酶的基因转移系统有利于将包括编码嵌合抗原受体的基因在内的基因整合到包括T细胞在内的免疫细胞的基因组中。
爪蟾转座子是用于修饰免疫细胞基因组的有利的piggyBac样转座子，并且包含如SEQ ID NO：6所示序列的ITR、待转座的异源多核苷酸和如SEQ ID NO：7所示序列的第二个ITR。转座子的每一侧侧翼还可以为四核苷酸5'-TTAA-3'的拷贝，紧邻ITR并远离异源多核苷酸。转座子还可以包含紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的一侧(优选左侧)与SEQ ID NO：1或2具有至少95％同一性；以及紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的另一侧(优选右侧)与SEQ ID NO：4或5具有至少95％同一性。该转座子可以通过转座酶进行转座，该转座酶包含与SEQ ID：31或32的序列具有至少90％同一性的序列，例如SEQ ID NO：33-63中的任一个。优选该转座酶是天然转座酶的高度变异体。优选地，该超活性变体转座酶相对于SEQ ID NO：31的序列包含以下氨基酸改变之一：Y6L、Y6H、Y6V、Y6I、Y6C、Y6G、Y6A、Y6S、Y6F、Y6R、Y6P、Y6D、Y6N、S7G、S7V、S7D、E9W、E9D、E9E、M16E、M16N、M16D、M16S、M16Q、M16T、M16A、M16L、M16H、M16F、M16I、S18C、S18Y、S18M、S18L、S18Q、S18G、S18P、S18A、S18W、S18H、S18K、S18I、S18V、S19C、S19V、S19L、S19F、S19K、S19E、S19D、S19G、S19N、S19A、S19M、S19P、S19Y、S19R、S19T、S19Q、S20G、S20M、S20L、S20V、S20H、S20W、S20A、S20C、S20Q、S20D、S20F、S20N、S20R、E21N、E21W、E21G、E21Q、E21L、E21D、E21A、E21P、E21T、E21S、E21Y、E21V、E21F、E21M、E22C、E22H、E22R、E22L、E22K、E22S、E22G、E22M、E22V、E22Q、E22A、E22Y、E22W、E22D、E22T、F23Q、F23A、F23D、F23W、F23K、F23T、F23V、F23M、F23N、F23P、F23H、F23E、F23C、F23R、F23Y、S24L、S24W、S24H、S24V、S24P、S24I、S24F、S24K、S24Y、S24D、S24C、S24N、S24G、S24A、S26F、S26H、S26V、S26Q、S26Y、S26W、S28K、S28Y、S28C、S28M、S28L、S28H、S28T、S28Q、V31L、V31T、V31I、V31Q、V31K、A34L、A34E、L67A、L67T、L67M、L67V、L67C、L67H、L67E、L67Y、G73H、G73N、G73K、G73F、G73V、G73D、G73S、G73W、G73L、A76L、A76R、A76E、A76I、A76V、D77N、D77Q、D77Y、D77L、D77T、P88A、P88E、P88N、P88H、P88D、P88L、N91D、N91R、N91A、N91L、N91H、N91V、Y141I、Y141M、Y141Q、Y141S、Y141E、Y141W、Y141V、Y141F、Y141A、Y141C、Y141K、Y141L、Y141H、Y141R、N145C、N145M、N145A、N145Q、N145I、N145F、N145G、N145D、N145E、N145V、N145H、N145W、N145Y、N145L、N145R、N145S、P146V、P146T、P146W、P146C、P146Q、P146L、P146Y、P146K、P146N、P146F、P146E、P148M、P148R、P148V、P148F、P148T、P148C、P148Q、P148H、Y150W、Y150A、Y150F、Y150H、Y150S、Y150V、Y150C、Y150M、Y150N、Y150D、Y150E、Y150Q、Y150K、H157Y、H157F、H157T、H157S、H157W、A162L、A162V、A162C、A162K、A162T、A162G、A162M、A162S、A162I、A162Y、A162Q、A179T、A179K、A179S、A179V、A179R、L182V、L182I、L182Q、L182T、L182W、L182R、L182S、T189C、T189N、T189L、T189K、T189Q、T189V、T189A、T189W、T189Y、T189G、T189F、T189S、T189H、L192V、L192C、L192H、L192M、L192I、S193P、S193T、S193R、S193K、S193G、S193D、S193N、S193F、S193H、S193Q、S193Y、V196L、V196S、V196W、V196A、V196F、V196M、V196I、S198G、S198R、S198A、S198K、T200C、T200I、T200M、T200L、T200N、T200W、T200V、T200Q、T200Y、T200H、T200R、S202A、S202P、L210H、L210A、F212Y、F212N、F212M、F212C、F212A、N218V、N218R、N218T、N218C、N218G、N218I、N218P、N218D、N218E、A248S、A248L、A248H、A248C、A248N、A248I、A248Q、A248Y、A248M、A248D、L263V、L263A、L263M、L263R、L263D、Q270V、Q270K、Q270A、Q270C、Q270P、Q270L、Q270I、Q270E、Q270G、Q270Y、Q270N、Q270T、Q270W、Q270H、S294R、S294N、S294G、S294T、S294C、T297C、T297P、T297V、T297M、T297L、T297D、E304D、E304H、E304S、E304Q、E304C、S308R、S308G、L310R、L310I、L310V、L333M、L333W、L333F、Q336Y、Q336N、Q336M、Q336A、Q336T、Q336L、Q336I、Q336G、Q336F、Q336E、Q336V、Q336C、Q336H、A354V、A354W、A354D、A354C、A354R、A354E、A354K、A354H、A354G、C357Q、C357H、C357W、C357N、C357I、C357V、C357M、C357R、C357F、C357D、L358A、L358F、L358E、L358R、L358Q、L358V、L358H、L358C、L358M、L358Y、L358K、L358N、L358I、D359N、D359A、D359L、D359H、D359R、D359S、D359Q、D359E、D359M、L377V、L377I、V423N、V423P、V423T、V423F、V423H、V423C、V423S、V423G、V423A、V423R、V423L、P426L、P426K、P426Y、P426F、P426T、P426W、P426V、P426C、P426S、P426Q、P426H、P426N、K428R、K428Q、K428N、K428T、K428F、S434A、S434T、S438Q、S438A、S438M、T447S、T447A、T447C、T447Q、T447N、T447G、L450M、L450V、L450A、L450I、L450E、A462M、A462T、A462Y、A462F、A462K、A462R、A462Q、A462H、A462E、A462N、A462C、V467T、V467C、V467A、V467K、I469V、I469N、I472V、I472L、I472W、I472M、I472F、L476I、L476V、L476N、L476F、L476M、L476C、L476Q、P488E、P488H、P488K、P488Q、P488F、P488M、P488L、P488N、P488D、Q498V、Q498L、Q498G、Q498H、Q498T、Q498C、Q498E、Q498M、L502I、L502M、L502V、L502G、L502F、E517M、E517V、E517A、E517K、E517L、E517G、E517S、E517I、P520W、P520R、P520M、P520F、P520Q、P520V、P520G、P520D、P520K、P520Y、P520E、P520L、P520T、S521A、S521H、S521C、S521V、S521W、S521T、S521K、S521F、S521G、N523W、N523A、N523G、N523S、N523P、N523M、N523Q、N523L、N523K、N523D、N523H、N523F、N523C、I533M、I533V、I533T、I533S、I533F、I533G、I533E、D534E、D534Q、D534L、D534R、D534V、D534C、D534M、D534N、D534A、D534G、D534F、D534T、D534H、D534K、D534S、F576L、F576K、F576V、F576D、F576W、F576M、F576C、F576R、F576Q、F576A、F576Y、F576N、F576G、F576I、F576E、K577L、K577G、K577D、K577R、K577H、K577Y、K577I、K577E、K577V、K577N、I582V、I582K、I582R、I582M、I582G、I582N、I582E、I582A、I582Q、Y583L、Y583C、Y583F、Y583D、Y583Q、L587F、L587D、L587R、L587I、L587P、L587N、L587E、L587S、L587Y、L587M、L587Q、L587G、L587W、L587K或L587T。
家蚕转座子是用于修饰免疫细胞基因组的有利的piggyBac样转座子，并且包含如SEQ ID NO：14所示序列的ITR、待转座的异源多核苷酸和如SEQ ID NO：15所示序列的第二个ITR。转座子的每一侧侧翼还可以为四核苷酸5'-TTAA-3'的拷贝，紧邻ITR并远离异源多核苷酸。转座子还可以包含紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的一侧(优选左侧)与SEQ ID NO：12具有至少95％同一性；以及紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的另一侧(优选右侧)与SEQ ID NO：13具有至少95％同一性。该转座子可以通过转座酶进行转座，该转座酶包含与SEQ ID NO：64具有至少90％同一性的序列，例如SEQ ID NO：65-86中的任一个。优选该转座酶是天然转座酶的高度变异体。优选地，该超活性变体转座酶相对于SEQ ID NO:BM-Tpase1的序列包含以下氨基酸改变之一：Q85E、Q85M、Q85K、Q85H、Q85N、Q85T、Q85F、Q85L、Q92E、Q92A、Q92P、Q92N、Q92I、Q92Y、Q92H、Q92F、Q92R、Q92D、Q92M、Q92W、Q92C、Q92G、Q92L、Q92V、Q92T、V93P、V93K、V93M、V93F、V93W、V93L、V93A、V93I、V93Q、P96A、P96T、P96M、P96R、P96G、P96V、P96E、P96Q、P96C、F97Q、F97K、F97H、F97T、F97C、F97W、F97V、F97E、F97P、F97D、F97A、F97R、F97G、F97N、F97Y、H165E、H165G、H165Q、H165T、H165M、H165V、H165L、H165C、H165N、H165D、H165K、H165W、H165A、E178S、E178H、E178Y、E178F、E178C、E178A、E178Q、E178G、E178V、E178D、E178L、E178P、E178W、C189D、C189Y、C189I、C189W、C189T、C189K、C189M、C189F、C189P、C189Q、C189V、A196G、L200I、L200F、L200C、L200M、L200Y、A201Q、A201L、A201M、L203V、L203D、L203G、L203E、L203C、L203T、L203M、L203A、L203Y、N207G、N207A、L211G、L211M、L211C、L211T、L211V、L211A、W215Y、T217V、T217A、T217I、T217P、T217C、T217Q、T217M、T217F、T217D、T217K、G219S、G219A、G219C、G219H、G219Q、Q235C、Q235N、Q235H、Q235G、Q235W、Q235Y、Q235A、Q235T、Q235E、Q235M、Q235F、Q238C、Q238M、Q238H、Q238V、Q238L、Q238T、Q238I、R242Q、K246I、K253V、M258V、F261L、S263K、C271S、N303C、N303R、N303G、N303A、N303D、N303S、N303H、N303E、N303R、N303K、N303L、N303Q、I312F、I312C、I312A、I312L、I312T、I312V、I312G、I312M、F321H、F321R、F321N、F321Y、F321W、F321D、F321G、F321E、F321M、F321K、F321A、F321Q、V323I、V323L、V323T、V323M、V323A、V324N、V324A、V324C、V324I、V324L、V324T、V324K、V324Y、V324H、V324F、V324S、V324Q、V324M、V324G、A330K、A330V、A330P、A330S、A330C、A330T、A330L、Q333P、Q333T、Q333M、Q333H、Q333S、P337W、P337E、P337H、P337I、P337A、P337M、P337N、P337D、P337K、P337Q、P337G、P337S、P337C、P337L、P337V、F368Y、L373C、L373V、L373I、L373S、L373T、V389I、V389M、V389T、V389L、V389A、R394H、R394K、R394T、R394P、R394M、R394A、Q395P、Q395F、Q395E、Q395C、Q395V、Q395A、Q395H、Q395S、Q395Y、S399N、S399E、S399K、S399H、S399D、S399Y、S399G、S399Q、S399R、S399T、S399A、S399V、S399M、R402Y、R402K、R402D、R402F、R402G、R402N、R402E、R402M、R402S、R402Q、R402T、R402C、R402L、R402V、T403W、T403A、T403V、T403F、T403L、T403Y、T403N、T403G、T403C、T403I、T403S、T403M、T403Q、T403K、T403E、D404I、D404S、D404E、D404N、D404H、D404C、D404M、D404G、D404A、D404Q、D404L、D404P、D404V、D404W、D404F、N408F、N408I、N408A、N408E、N408M、N408S、N408D、N408Y、N408H、N408C、N408Q、N408V、N408W、N408L、N408P、N408K、S409H、S409Y、S409N、S409I、S409D、S409F、S409T、S409C、S409Q、N441F、N441R、N441M、N441G、N441C、N441D、N441L、N441A、N441V、N441W、G448W、G448Y、G448H、G448C、G448T、G448V、G448N、G448Q、E449A、E449P、E449T、E449L、E449H、E449G、E449C、E449I、V469T、V469A、V469H、V469C、V469L、L472K、L472Q、L472M、C473G、C473Q、C473T、C473I、C473M、R484H、R484K、T507R、T507D、T507S、T507G、T507K、T507I、T507M、T507E、T507C、T507L、T507V、G523Q、G523T、G523A、G523M、G523S、G523C、G523I、G523L、I527M、I527V、Y528N、Y528W、Y528M、Y528Q、Y528K、Y528V、Y528I、Y528G、Y528D、Y528A、Y528E、Y528R、Y543C、Y543W、Y543I、Y543M、Y543Q、Y543A、Y543R、Y543H、E549K、E549C、E549I、E549Q、E549A、E549H、E549C、E549M、E549S、E549F、E549L、K550R、K550M、K550Q、S556G、S556V、S556I、P557W、P557T、P557S、P557A、P557Q、P557K、P557D、P557G、P557N、P557L、P557V、H559K、H559S、H559C、H559I、H559W、V560F、V560P、V560I、V560H、V560Y、V560K、N561P、N561Q、N561G、N561A、V562Y、V562I、V562S、V562M、V567I、V567H、V567N、S583M、E601V、E601F、E601Q、E601W、E605R、E605W、E605K、E605M、E605P、E605Y、E605C、E605H、E605A、E605Q、E605S、E605V、E605I、E605G、D607V、D607Y、D607C、D607N、D607W、D607T、D607A、D607H、D607Q、D607E、D607L、D607K、D607G、S609R、S609W、S609H、S609V、S609Q、S609G、S609T、S609K、S609N、S609Y、L610T、L610I、L610K、L610G、L610A、L610W、L610D、L610Q、L610S、L610F或L610N。
piggyBat转座子是用于修饰免疫细胞基因组的有利的piggyBac样转座子，并且包含如SEQ ID NO：20所示序列的ITR、待转座的异源多核苷酸和如SEQ ID NO：21所示序列的第二个ITR。转座子的每一侧侧翼还可以为四核苷酸5'-TTAA-3'的拷贝，紧邻ITR并远离异源多核苷酸。转座子还可以包含紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的一侧(优选左侧)与SEQ ID NO：22具有至少95％同一性；以及紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的另一侧(优选右侧)与SEQ ID NO：23具有至少95％同一性。该转座子可以通过转座酶进行转座，该转座酶包含与SEQ ID NO：29具有至少90％同一性的序列。优选该转座酶是天然转座酶的高度变异体。优选地，该超活性变体转座酶相对于SEQ IDNO：29的序列包含以下氨基酸改变之一：A14V、D475G、P491Q、A561T、T546T、T300A、T294A、A520T、G239S、S5P、S8F、S54N、D9N、D9G、1345V、M481V、EI lG、K130T、G9G、R427H、S8P、S36G、DlOG、S36G。
用于修饰免疫细胞基因组的有利的piggyBac样转座子包含如SEQ ID NO：16所示序列的ITR、待转座的异源多核苷酸和如SEQ ID NO：17所示序列的第二ITR。转座子的每一侧侧翼还可以为四核苷酸5'-TTAA-3'的拷贝，紧邻ITR并远离异源多核苷酸。转座子还可以包含紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的一侧(优选左侧)与SEQ IDNO：18具有至少95％同一性；以及紧邻ITR并邻近异源多核苷酸的序列，其在异源多核苷酸的另一侧(优选右侧)与SEQ ID NO：19具有至少95％同一性。该转座子可以通过转座酶进行转座，该转座酶包含与SEQ ID NO：30具有至少90％同一性的序列。优选该转座酶是天然转座酶的高度变异体。优选地，该超活性变体转座酶相对于SEQ ID NO：30的序列包含以下氨基酸改变之一：G2C、Q40R、I30V、G165S、T43A、S61R、S103P、S103T、M194V、R281G、M282V、G316E、I426V、Q497L、N505D、Q573L、S509G、N570S、N538K、Q591P、Q591R、F594L、M194V、I30V、S103P、G165S、M282V、S509G、N538K、N571S、C41T、A1424G、C1472A、G1681A、T150C、A351G、A279G、T1638C、A898G、A880G、G1558A、A687G、G715A、T13C、C23T、G161A、G25A、T1050C、A1356G、A26G、A1033G、A1441G、A32G、A389C、A32G、A389C、A32G、T1572A、G456A、T1641C、Tl155C、G1280A、T22C、A106G、A29G、C137T、A14V、D475G、P491Q、A561T、T546T、T300A、T294A、A520T、G239S、S5P、S8F、S54N、D9N、D9G、1345V、M481V、E1lG、K130T、G9G、R427H、S8P、S36G、Dl0G、S36G、A51T、C153A、C277T、G201A、G202A、T236A、A103T、A104C、T140C、G138T、T118A、C74T、A179C、S3N、I30V、A46S、A46T、I82W、S103P、R119P、C125A、C125L、G165S、Y177K、Y177H、F180L、F180I、F180V、M185L、A187G、F200W、V207P、V209F、M226F、L235R、V240K、F241L、P243K、N258S、M282Q、L296W、L296Y、L296F、M298V、M298A、M298L、P311V、P311I、R315K、T319G、Y327R、Y328V、C340G、C340L、D421H、V436I、M456Y、L470F、S486K、M503I、M503L、V552K、A570T、Q591P、Q591R、R65A、R65E、R95A、R95E、R97A、R97E、R135A、R135E、R161A、R161E、R192A、R192E、R208A、R208E、K176A、K176E、K195A、K195E、S171E、M14V、D270N、I30V、G165S、M282L、M282I、M282V或M282A。
用于修饰免疫细胞基因组的有利的Mariner转座子是睡美人转座子，其包含如SEQID NO：26所示序列的ITR、异源多核苷酸和如SEQ ID NO：27所示序列的第二ITR。ITR可以是较长转座子末端序列的一部分，例如转座子可以包含与SEQ ID NO：24具有至少95％同一性的序列的左端和与SEQ ID NO：25具有至少95％同一性的序列的右端。转座子可以被转座酶转座，该转座酶包含与SEQ ID NO：28具有至少90％同一性的序列，包括其超活性变体。
用于修饰免疫细胞基因组的有利的hAT转座子为TcBuster转座子，其包含如SEQID NO：399所示序列的ITR、异源多核苷酸和如SEQ ID NO：400所示序列的第二ITR。ITR可以是较长转座子末端序列的一部分，例如转座子可以包含与SEQ ID NO：397具有至少95％同一性的序列的左端和与SEQ ID NO：398具有至少95％同一性的序列的右端。转座子可以被转座酶转座，该转座酶包含与SEQ ID NO：401具有至少90％同一性的序列，包括其超活性变体。
转座酶蛋白可以作为蛋白或作为编码转座酶的核酸，例如，作为核糖核酸，包括mRNA或由细胞的翻译机器识别的任何多核苷酸；作为DNA，例如作为染色体外DNA，包括附加体DNA；作为质粒DNA或作为病毒核酸导入细胞。此外，编码转座酶蛋白的核酸可以作为核酸载体(例如质粒)或作为基因表达载体(包括病毒载体)转染到细胞中。核酸可以是环状的或线性的。编码转座酶蛋白的DNA可以稳定地插入细胞的基因组或用于组成型或诱导型表达的载体中。当转座酶蛋白转染入细胞或作为DNA插入载体时，转座酶编码序列优选与异源启动子可操作地连接。存在多种可使用的启动子，包括组成型启动子、组织特异性启动子、诱导型启动子等。编码piggyBac样转座酶蛋白的所有DNA或RNA序列都是明确考虑的。或者，例如，可以使用细胞穿透肽(例如，如Ramsey和Flynn(2015)Pharmacol.Ther.15:78-86“Cell-penetrating peptides transport therapeutics into cells(细胞穿透肽将治疗剂转运到细胞中)”中所述)；使用小分子，包括盐和丙基甜菜碱(例如，如Astolfo等人(2015)Cell161：674-690中所述)；或电穿孔(例如，如Morgan和Day(1995)Methods in MolecularBiology 48:63-71“通过电穿孔将蛋白质导入哺乳动物细胞中”中所述)将转座酶作为蛋白直接导入细胞中。
5.2.2基因转移系统
基因转移系统包含待转移至宿主细胞的多核苷酸。基因转移系统可以包含本文所述的任何转座子或转座酶，或者它可以包含一种或多种多核苷酸，所述多核苷酸具有促进有效基因转移而不需要转座酶或转座子的其他特征。
当存在基因转移系统的多个组分时，例如包含用于在靶细胞中表达的基因并且任选地包含转座子末端的一种或多种多核苷酸，以及转座酶(其可以作为蛋白质提供或由核酸编码)，这些组分可以同时或在不同时间转染到细胞中。例如，可以在转染相应的转座子之前、同时或之后，将转座酶蛋白或其编码核酸转染到细胞中。另外，基因转移系统的任一组分的施用可重复进行，例如，通过施用至少两个剂量的这种组分。
转座酶蛋白可以由包括RNA或DNA的多核苷酸编码。如果转座酶以DNA编码的基因形式提供，则其应当优选与在靶细胞中有活性的启动子可操作地连接。优选的RNA分子包括具有适当取代以降低对细胞的毒性作用的那些，例如用假尿苷取代尿苷和用5-甲基胞嘧啶取代胞嘧啶。类似地，可以将转座子或编码本发明转座酶的核酸作为线性片段或作为环化片段、作为质粒或作为重组病毒DNA转染到细胞中。
通过诸如粒子轰击、电穿孔、显微注射等技术、将组分与脂质纳米颗粒或含脂质囊泡如阳离子脂质囊泡、DNA缩合试剂(例如，磷酸钙、聚赖氨酸或聚乙烯亚胺)、或将其组分(即核酸)插入病毒载体并使病毒载体与细胞接触，可以将所述基因转移系统的组分转染到一个或多个细胞中。当使用病毒载体时，病毒载体可以包括本领域已知的多种病毒载体中的任何一种，包括选自逆转录病毒载体、腺病毒载体或腺相关病毒载体的病毒载体。基因转移系统可以以本领域已知的合适方式配制、或配制为药物组合物或试剂盒。
5.2.3启动子元件
用于在免疫细胞中表达多肽的基因转移系统包含待转移至宿主细胞的多核苷酸。多核苷酸包含在免疫细胞中有活性的启动子。实例包括来自组成型表达基因的启动子，该组成型表达基因包括哺乳动物甘油醛3-磷酸脱氢酶(GAPDH)基因(例如SEQ ID NO：97-107的序列)、哺乳动物磷酸甘油酸激酶(PGK)基因(例如SEQ ID NO：115-118的序列)、哺乳动物延伸因子1a(EF1a)基因(例如SEQ ID NO：94、96和128-146的序列)、哺乳动物延伸因子2(EEF2)基因(例如SEQ ID NO：1108、109、114和147-154的序列)和泛素基因(例如SEQ IDNO：95或125-127的序列)。这些基因可以与或不与内含子序列(包括它们的天然内含子序列)一起使用。示例性内含子序列如SEQ ID NO：155-159所示。
5.2.4聚腺苷酸化元件
基因转移系统可用于将用于表达的基因导入真核细胞。许多真核细胞，包括动物细胞和高等植物细胞，加工基因表达过程中转录的mRNA。编码蛋白质的基因通常是多腺苷酸化的，其稳定细胞内的mRNA。聚腺苷酸化信号也可以帮助终止转录。当要从多核苷酸表达一个以上的开放阅读框时，这可能特别有用，因为它有助于减少两个启动子之间的干扰。可以合成设计聚腺苷酸化序列，其有效终止从一个启动子的转录，从而减少对位于第一启动子3'的第二启动子的干扰。序列SEQ ID NO：160-217都可用于起始转录序列的聚腺苷酸化和终止转录。聚腺苷酸化序列SEQ ID NO：160-217可包括在用于在包括脊椎动物或无脊椎动物细胞的动物细胞中表达基因的基因转移系统的多核苷酸中。聚腺苷酸化序列SEQ IDNO：160-217可用于在脊椎动物细胞中表达基因，脊椎动物细胞包括来自哺乳动物的细胞，哺乳动物包括啮齿动物如大鼠、小鼠和仓鼠；有蹄类动物如牛、山羊或绵羊；猪；以及来自人组织和人干细胞的细胞。聚腺苷酸化序列SEQ ID NO：160-217可用于不同的细胞类型，包括免疫细胞、淋巴细胞、肝细胞、神经细胞、肌细胞、血细胞、胚胎干细胞、体干细胞、造血细胞、胚胎、受精卵和精子细胞(其中一些是开放的以在体外环境中操作)中。聚腺苷酸化序列SEQID NO：160-217可用于在多能细胞(其后代可分化成几种限制性细胞类型的细胞，例如造血干细胞或其他干细胞)或全能细胞(即，其后代可变成生物体中任何细胞类型的细胞，例如胚胎干细胞)中表达基因。聚腺苷酸化序列SEQ ID NO：160-217可用于在培养细胞如中国仓鼠卵巢(CHO)细胞或人胚肾(HEK293)细胞中表达基因。
可以将SEQ ID NO：160-217的聚腺苷酸化序列掺入piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster、或基于非转座子的基因递送多核苷酸中。优选将聚腺苷酸化序列SEQ ID NO：160-217掺入多核苷酸中待表达的开放阅读框的3'端。当置于待表达的两个基因之间时，聚腺苷酸化序列SEQ ID NO：160-217可用于终止来自第一个启动子的转录并减少启动子干扰。有利的基因转移系统包含与SEQ ID NO：160-217的任一个具有至少80％或90％或95％或96％或97％或98％或99％或100％同一性的序列。
5.2.5绝缘子元件
当异源多核苷酸整合到免疫细胞的基因组中时，通常需要防止异源多核苷酸内的遗传元件影响内源免疫细胞基因的表达。类似地，通常需要防止异源多核苷酸内的基因受免疫细胞基因组中的元件影响，例如通过掺入异染色质中而沉默。已知绝缘子元件具有增强子阻断活性(有助于防止异源多核苷酸中的基因影响内源免疫细胞基因的表达)和屏障活性(有助于防止异源多核苷酸中的基因通过掺入异染色质而沉默)。增强子阻断活性可由转录抑制子CTCF蛋白的结合产生。屏障活性可由脊椎动物屏障蛋白如USF1和VEZF1的结合产生。有用的绝缘子序列包含CTCF、USF1或VEZF1的结合位点。有利的基因转移系统包含含有绝缘子序列的多核苷酸，绝缘子序列包含CTCF、USF1或VEZF1的结合位点。更优选地，基因转移系统包含含有两个绝缘子序列的多核苷酸，每个绝缘子序列包含CTCF、USF1或VEZF1的结合位点，其中两个绝缘子序列位于异源多核苷酸内的任何启动子或增强子的侧翼。绝缘子序列的有利实例如SEQ ID NO：87-93所示。
如果将包含启动子或增强子的异源多核苷酸整合到没有绝缘子序列的免疫细胞的基因组中，则存在异源多核苷酸内的启动子或增强子元件将影响内源免疫细胞基因(例如癌基因)的表达、或者异源多核苷酸内的启动子或增强子元件将通过掺入异染色质中而沉默的风险。当异源多核苷酸在随机片段化之后整合到靶基因组中时，一些遗传元件经常丢失，而其他遗传元件可以重排。因此，存在显著的风险：如果异源多核苷酸包含侧翼为增强子和启动子元件的绝缘子元件，则绝缘子元件可能重排或丢失，并且增强子和启动子元件可能能够影响它们所整合的基因组环境并受其影响。因此，使用转座子基因转移系统是有利的，其中两个转座子ITR之间的完整序列被整合到免疫细胞基因组中，而没有重排。因此，用于整合到免疫细胞基因组中的有利的基因转移系统包括转座子，其中元件按以下顺序排列：左转座子末端；第一绝缘子序列；用于在免疫细胞内表达的序列；第二绝缘子序列；右转座子末端。用于在免疫细胞内表达的序列可以包括与任何数量的开放阅读框有效连接的任何数量的调控序列。转座子末端优选是piggyBac样转座子或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子的末端。
5.3用于增强免疫细胞存活的遗传元件
为了使免疫细胞充分应答对身体的威胁，它们必须能够存活足够长的时间以攻击它们的靶标。对于需要离体操作免疫细胞的治疗和研究，免疫细胞增殖是有利的。然而，离体培养条件和某些体内环境(例如实体瘤内的环境)对于免疫细胞的生长都不是最佳的。例如，当用抗CD19嵌合抗原受体工程改造时，来自重度预处理的淋巴瘤患者的T细胞显示比来自未处理患者的T细胞更低的离体扩增和临床应答率。因此，需要一种增强人免疫细胞的功能、持久性和增殖的方法，特别是在天然对抗免疫细胞的条件下。
5.3.1 T细胞转化元件
增强人免疫细胞的持久性和增殖的一种方法是将增加生长和/或存活的遗传元件整合到免疫细胞的基因组中。用于增强免疫细胞存活的候选遗传元件包括在免疫细胞癌中发现突变的基因。然而，通常认为将细胞转化为癌细胞需要一系列突变，并且每个突变的作用可能与细胞存活或生长不直接相关。例如，已知许多突变仅仅增加发生额外突变的机会。因此，即使可能存在某些基因中的突变经常发生在免疫细胞癌中的相关性，但将相同的突变基因导入免疫细胞通常不会增强增强该细胞的生长或存活。因此可以进行测试以确定包含含有天然突变的基因的异源多核苷酸整合到免疫细胞的基因组中是否将增加该细胞的存活和增殖。
我们试图鉴定可以在异源多核苷酸上提供并整合到免疫细胞的基因组中的基因，以赋予该免疫细胞生长或存活益处。为此，我们合成了包含具有编码天然存在的突变人蛋白质的序列的基因的多核苷酸，该突变人蛋白质包括可操作地连接至对免疫细胞中蛋白质的表达有效的异源启动子的激活突变，并将这些异源多核苷酸整合至T细胞的基因组中。然后如6.2节所述，我们测定了这些T细胞在离体培养中的生长和存活。
5.3.1.1 STAT3
编码STAT3(信号转导子和转录激活子3)的基因在大颗粒淋巴细胞白血病中常发生突变。这些激活突变通常位于STAT3的SH2结构域中，并且包括S614R、E616K、G618R、Y640F、N647I、E652K、K658Y、K658R、K658N、K658M、K658R、K658H、K658N、D661Y和D661V。在SH2结构域外还发现了STAT3中的激活突变，例如F174S和H410R。如6.2.1.1和6.2.1.5节所述，我们已经证明编码STAT3蛋白的激活突变体的异源多核苷酸可以被导入免疫细胞中以增强其存活或其增殖；编码STAT3的激活突变体的基因是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含STAT3修饰形式的蛋白(例如SEQ ID NO：232)的多核苷酸，其序列包含选自F174S、H410R、S614R、E616K、G618R、Y640F、N647I、E652K、K658Y、K658R、K658N、K658M、K658R、K658H、K658N、D661Y和D661V的一个或多个突变。示例性突变的STAT3蛋白包括SEQID NO：246-250。优选的实施方案包括包含编码STAT3蛋白(包含激活突变)的核酸的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码STAT3的激活突变体的核酸可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码STAT3的激活突变体的核酸的多核苷酸，其中该核酸可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码STAT3的激活突变体的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ IDNO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码STAT3的激活突变体的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码STAT3的激活突变体的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码STAT3的激活突变体的多核苷酸的转座子与相应的转座酶或编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码STAT3的激活突变体的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码突变的STAT3蛋白的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码具有激活突变的STAT3蛋白的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含STAT3基因的3个拷贝：两个内源拷贝和一个异源突变拷贝。
5.3.1.2 CD28
CD28(分化簇28)基因经常在外周T细胞淋巴瘤中发现突变。最常见的激活突变是D124E、D124V、T195I和T195P。如6.2.1.2和6.2.1.3节所述，我们已经证明编码CD28蛋白的激活突变体的异源多核苷酸可以被导入免疫细胞以增强其存活或其增殖，并减少再刺激诱导的细胞死亡；编码CD28的激活突变体的基因是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含CD28修饰形式的蛋白(例如，SEQ ID NO：233)的多核苷酸，其序列包含选自D124E、D124V、T195I和T195P的一个或多个突变。示例性突变的CD28蛋白如SEQ ID NO：251所示。突变CD28还可包含用另一功能活性分泌信号替换SEQ ID NO：233的前18个氨基酸中的分泌信号。优选的实施方案包括包含编码CD28的激活突变体的核酸的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码突变的CD28的核酸可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码CD28的激活突变体的核酸的多核苷酸，其中该核酸可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码CD28的激活突变体的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ IDNO：20和21所示的序列。优选的实施方案包括包含编码CD28的激活突变体的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码CD28的激活突变体的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码CD28激活突变体的多核苷酸的转座子与相应的转座酶或编码相应转座酶的多核苷酸一起导入免疫细胞。优选的实施方案包括包含编码CD28的激活突变体的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码CD28的激活突变体的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码具有激活突变的CD28蛋白的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含CD28基因的3个拷贝：两个内源拷贝和一个异源突变拷贝。
5.3.1.3 RhoA
RhoA小GTP酶在外周T细胞淋巴瘤中经常发生突变。最常见的淋巴瘤相关突变是G17V和K18N。可以将RhoA蛋白的激活突变体导入免疫细胞以增强其存活或增殖；编码RhoA的激活突变体的基因是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含RhoA的修饰形式的蛋白(例如，SEQ ID NO：234)的多核苷酸，其序列包含选自G17V和K18N的突变或其组合。示例性突变的RhoA蛋白如SEQ IDNO：252和253所示。优选的实施方案包括包含编码突变的RhoA蛋白的核酸的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码突变的RhoA的基因可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码突变的RhoA蛋白的核酸的多核苷酸，其中该核酸可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码突变的RhoA蛋白的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码突变的RhoA蛋白的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码突变的RhoA蛋白的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码突变的RhoA蛋白的多核苷酸的转座子与编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码突变的RhoA蛋白的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码突变的RhoA蛋白的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码具有激活突变的RhoA蛋白的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含RhoA基因的3个拷贝：两个内源拷贝和一个异源突变拷贝。
5.3.1.4磷脂酶C，γ1
激活磷脂酶Cγ(PLCG)突变与皮肤T细胞淋巴瘤相关。最常见的淋巴瘤相关激活突变为S345F、S520F和R707Q。如6.2.1.5节中所述，我们已经证明编码PLCG蛋白的激活突变体的异源多核苷酸可以被导入免疫细胞以增强其存活或其增殖；编码PLCG的激活突变体的基因是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含PLCG修饰形式的蛋白(例如，SEQ ID NO：235)的多核苷酸，其序列包含选自S345F、S520F和R707Q的一个或多个突变。示例性的突变PLCG蛋白如SEQ ID NO：254所示。优选的实施方案包括包含编码PLCG的激活突变体的基因的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码突变的PLCG的基因可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码PLCG的激活突变体的核酸的多核苷酸，其中该核酸可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码PLCG的激活突变体的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码PLCG的激活突变体的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码突变的PLCG蛋白的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码突变的PLCG蛋白的多核苷酸的转座子与相应的转座酶或编码相应的转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码突变的PLCG蛋白的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码突变的PLCG蛋白的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码具有激活突变的PLCG蛋白的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含PLCG基因的3个拷贝：两个内源拷贝和一个异源突变拷贝。
5.3.1.5 STAT5B
编码STAT5B(信号转导和转录激活因子5B)的基因在T细胞白血病中有时发生突变。最常见的白血病相关激活突变是SH2结构域中的N642H。与T细胞癌症相关的其他STAT5B激活突变包括SH2结构域突变T648S、S652Y和Y665F，以及SH2结构域外的P267A。可以将编码STAT5B蛋白的激活突变体的异源多核苷酸导入免疫细胞中以增强其存活或其增殖；编码STAT5B的激活突变体的基因是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含STAT5B修饰形式的蛋白(例如，SEQ ID NO：236)的多核苷酸，其序列包含选自N642H、T648S、S652Y、Y665F和P267A的一个或多个突变。示例性突变的STAT5B蛋白如SEQ ID NO：255所示。优选的实施方案包括包含编码突变的STAT5B蛋白的核酸的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码突变的STAT5B的基因可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码突变的STAT5B蛋白的基因的多核苷酸，其中该基因可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码突变的STAT5B蛋白的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码STAT5B的激活突变体的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码突变的STAT5B蛋白的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码突变的STAT5B蛋白的多核苷酸的转座子与相应的转座酶或编码相应的转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码突变的STAT5B蛋白的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码突变的STAT5B蛋白的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码具有激活突变的STAT5B蛋白的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含STAT5B基因的3个拷贝：两个内源拷贝和一个异源突变拷贝。
5.3.1.6生存素
编码生存素(Survivin)(凋亡蛋白家族抑制因子的成员)的基因在T细胞白血病中有时被上调。如6.2.1.3节所述，我们已经证明，编码可操作地连接到异源启动子的生存素基因的异源多核苷酸可以被导入免疫细胞以增强其存活或其增殖，并减少再刺激诱导的细胞死亡；可操作地连接到异源启动子的生存素基因是如6.2节所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含可操作地连接到异源启动子的SEQ ID NO：237的蛋白质的多核苷酸。可与编码生存素的基因可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码生存素的基因的多核苷酸，其中该基因可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码生存素的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ IDNO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码生存素的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码生存素的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码生存素的多核苷酸的转座子与相应的转座酶或编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码生存素的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码生存素的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞或B细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码生存素的基因并且还包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含生存素基因的3个拷贝：与异源启动子可操作地连接的两个内源拷贝和一个异源拷贝。
5.3.1.7 Bcl-XL
编码Bcl-XL(一种抗凋亡蛋白)的基因在B细胞淋巴瘤中有时上调。如6.2.1.5节和6.2.1.6节中所述，我们已经证明，编码可操作地连接到异源启动子的Bcl-XL基因的异源多核苷酸可以被导入免疫细胞以增强其存活或其增殖，并减少再刺激诱导的细胞死亡；可操作地连接到异源启动子的Bcl-XL基因是如6.2节所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含可操作地连接到异源启动子的SEQ IDNO：238的蛋白质的多核苷酸。可与编码Bcl-XL的核酸可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码Bcl-XL蛋白的核酸的多核苷酸，其中该核酸可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码Bcl-XL的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码Bcl-XL的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码Bcl-XL的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码Bcl-XL的多核苷酸的转座子与编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码Bcl-XL基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码Bcl-XL的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞或B细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码Bcl-XL的基因并且还包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含Bcl-XL基因的3个拷贝：与异源启动子可操作地连接的两个内源拷贝和一个异源拷贝。
5.3.1.8CCND1
编码CCND1(细胞周期蛋白D1)的基因在白血病中有时发生突变。与癌症相关的CCND1突变包括E36G、E36Q、E36K、A39S、S41L、S41P、S41T、V42E、V42A、V42L、V42M、Y44S、Y44D、Y44C、Y44H、K46T、K46R、K46N、K46E、C47G、C47R、C47S、C47W、P199R、P199S、P199L、S201F、T285I、T285A、P286L、P286H、P286S、P286T和P286A。可以将编码CCND1蛋白的激活突变体的异源多核苷酸导入免疫细胞以增强其存活或其增殖；编码CCND1的激活突变体的基因是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含CCND1修饰形式的蛋白(例如，SEQ ID NO：239)的多核苷酸，其序列包含选自E36G、E36Q、E36K、A39S、S41L、S41P、S41T、V42E、V42A、V42L、V42M、Y44S、Y44D、Y44C、Y44H、K46T、K46R、K46N、K46E、C47G、C47R、C47S、C47W、P199R、P199S、P199L、S201F、T285I、T285A、P286L、P286H、P286S、P286T和P286A的一个或多个突变。示例性突变的CCND1蛋白如SEQ ID NO：256所示。优选的实施方案包括包含编码突变的CCND1蛋白的核酸的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码突变的CCND1的核酸可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码突变的CCND1蛋白的核酸的多核苷酸，其中该核酸可操作地连接到异源聚腺苷酸化信号，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码突变的CCND1蛋白的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码CCND1的激活突变体的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码CCND1的激活突变体的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码突变的CCND1蛋白的多核苷酸的转座子与编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码突变的CCND1蛋白的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码突变的CCND1蛋白的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞或B细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码具有激活突变的CCND1蛋白的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含CCND1基因的3个拷贝：两个内源拷贝和一个异源突变拷贝。
5.3.1.9 Bcl2
编码Bcl2(一种抗凋亡蛋白)的基因在B细胞淋巴瘤中有时上调。如6.2.1.4节所述，我们已经证明，编码可操作地连接到异源启动子的Bcl2基因的异源多核苷酸可以被导入免疫细胞以增强其存活或其增殖；可操作地连接到异源启动子的Bcl2基因是如6.2节所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含可操作地连接到异源启动子的SEQ ID NO：v270或272的蛋白质的多核苷酸。可与编码Bcl2的核酸可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码Bcl2的核酸的多核苷酸，其中该核酸与异源聚腺苷酸化信号可操作地连接，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码Bcl2的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码Bcl2的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码Bcl2的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码Bcl2的多核苷酸的转座子与编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码Bcl2的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码Bcl2的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞或B细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码Bcl2的基因并且还包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。在一些实施方案中，免疫细胞基因组包含Bcl2基因的3个拷贝：与异源启动子可操作地连接的两个内源拷贝和一个异源拷贝。
5.3.1.10 Bcl6
编码Bcl6(一种抗凋亡蛋白)的基因在B细胞淋巴瘤中有时上调。如6.2.1.4节所述，我们已经证明，编码可操作地连接到异源启动子的Bcl6基因的异源多核苷酸可以被导入免疫细胞以增强其存活或其增殖，并减少再刺激诱导的细胞死亡；可操作地连接到异源启动子的Bcl6基因是如6.2节所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。本发明的一个实施方案是编码包含可操作地连接到异源启动子的SEQ ID NO：271或272的蛋白质的多核苷酸。可与编码Bcl6的核酸可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码Bcl6的核酸的多核苷酸，其中该核酸与异源聚腺苷酸化信号可操作地连接，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。优选的实施方案包括包含编码Bcl6的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码Bcl6的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码Bcl6的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ IDNO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码Bcl6的多核苷酸的转座子与相应的转座酶或编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码Bcl6的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码Bcl6的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞或B细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码Bcl6的基因并且还包含慢病毒载体或piggyBac样转座子。在一些实施方案中，免疫细胞基因组包含Bcl6基因的3个拷贝：与异源启动子可操作地连接的两个内源拷贝和一个异源拷贝。
5.3.2增强型信号传导受体
免疫细胞例如T细胞表达膜蛋白，该膜蛋白包含结合天然存在的和合成的配体的胞外结构域、跨膜结构域以及与细胞内信号传导通路相互作用的胞内结构域。我们已经设计、合成和测试了一组嵌合受体，我们称其为增强型信号传导受体(ESRs)，其包含衍生自第一蛋白的胞外结构域、跨膜结构域和衍生自将刺激或共刺激信号传递至免疫细胞的受体的胞内结构域。然而，与嵌合抗原受体不同，ESRs不包含含有CD3ζ链的细胞内部分的序列。ESRs的一个功能是增强免疫细胞存活。ESR的另一个功能是例如通过作用于抑制性受体的肿瘤细胞来抵消T细胞抑制性通路的参与(Tay等人，2017.Immunotherapy 9，1339-1349)。为了使ESR有效发挥功能，它们必须以足够高的水平表达，以与天然抑制性受体竞争肿瘤微环境内存在的抑制性配体。
在一个实施方案中，ESR的胞外结构域可以衍生自向免疫细胞天然传递抑制信号的受体的胞外配体结合结构域：在这种情况下，ESR接收通常解释为抑制信号的信号并将其转导为刺激信号。例如，ESR的胞外结构域可以包含衍生自选自以下的蛋白质的胞外结构域的序列：TNFRSF1A、TNFRSF3(LTRβ)、TNFRSF6(Fas)、TNFRSF8(CD30)、TNFRSF10A(DR4)、TNFRSF10B(DR5)、TNFRSF19(TROY)、TNFRSF21(DR6)和CTLA4；优选地，胞外结构域衍生自人蛋白。在本发明的一些实施方案中，ESR的胞外结构域包含多肽，该多肽的序列与选自SEQID NO：322-330的序列具有至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％或100％同一性。
在另一个实施方案中，ESR的胞外结构域可能衍生自与免疫细胞表面表达的蛋白质结合的蛋白质，优选其正常功能是刺激免疫功能的蛋白质：在这种情况下，ESR将刺激信号传递至另一种免疫细胞并将刺激信号转导至其所表达的免疫细胞。例如，ESR胞外结构域可包含抗体、单链抗体、单域抗体、纳米抗体、VHH片段或VNAR片段的可变域，其结合选自以下的蛋白质的胞外结构域：TNFRSF4(OX40)、TNFRSF5(CD40)、TNFRSF7(CD27)、TNFRSF9(4-1BB)、TNFRSF11A(RANK)、TNFRSF13B(TACI)、TNFRSF13C(BAFF-R)、TNFRSF14(HVEM)、TNFRSF17(CD269)、TNFRSF18(GITR)、CD28、CD28H(TMIGD2)、诱导型T细胞共刺激因子(ICOS/CD278)、DNAX辅助分子1(DNAM-1/CD226)、信号转导淋巴细胞激活分子(SLAM/CD150)、T细胞免疫球蛋白和粘蛋白结构域(TIM-1/HAVcr-1)、干扰素受体α链(IFNAR1)、干扰素受体β链(IFNAR2)、白细胞介素2受体β亚基(IL2RB)和白细胞介素2受体γ亚基(IL2RG)。示例性的单链抗CD28抗体为TTGN1412，其序列如SEQ ID NO：340所示。
在另一个实施方案中，ESR的胞外结构域可能衍生自与免疫细胞表面表达的受体结合的配体，优选其正常功能是转导免疫细胞中的刺激或共刺激信号的受体：在这种情况下，ESR将刺激信号传递至另一种免疫细胞并将刺激信号转导至其所表达的免疫细胞。例如，ESR胞外结构域可包含衍生自选自以下的蛋白的胞外结构域的序列：TNFSF4(OX40配体)、TNFSF5(CD40配体)、TNFSF9(4-1BB配体)、TNFSF11(RANKL)、TNFSF14(HVEM配体)、TNFSF13B、CD80、CD86和ICOS配体；优选地，胞外结构域衍生自人蛋白。在本发明的一些实施方案中，ESR的胞外结构域包含多肽，该多肽的序列与选自SEQ ID NO：331-339的序列具有至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％同一性。
在本发明的一些实施方案中，增强型信号传导受体包含衍生自肿瘤坏死因子受体超家族(TNFRSF)成员或通常将刺激信号传递至免疫细胞的另一种免疫细胞受体的胞内结构域的序列；在本发明的一些实施方案中，ESR包含衍生自选自以下蛋白质的胞内结构域的序列：TNFRSF4(OX40)、TNFRSF5(CD40)、TNFRSF7(CD27)、TNFRSF9(4-1BB)、TNFRSF11A(RANK)、TNFRSF13B(TACI)、TNFRSF13C(BAFF-R)、TNFRSF14(HVEM)、TNFRSF17(CD269)、TNFRSF18(GITR)、CD28、CD28H(TMIGD2)、诱导型T细胞共刺激因子(ICOS/CD278)、DNAX辅助分子1(DNAM-1/CD226)、信号转导淋巴细胞激活分子(SLAM/CD150)、T细胞免疫球蛋白和粘蛋白结构域(TIM-1/HAVcr-1)、干扰素受体α链(IFNAR1)、干扰素受体β链(IFNAR2)、白细胞介素2受体β亚基(IL2RB)、白细胞介素2受体γ亚基(IL2RG)、肿瘤坏死因子超家族14(TNFSF14/LIGHT)、自然杀伤细胞2族成员D(NKG2D/CD314)和CD40配体(CD40L)；优选地，胞内结构域为人蛋白的胞内结构域。在本发明的一些实施方案中，ESR包含多肽，该多肽的序列与选自SEQ ID NO：341-364的序列具有至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％或100％同一性。
在本发明的一些实施方案中，增强型信号传导受体包含衍生自肿瘤坏死因子受体超家族(TNFRSF)成员或通常将抑制或刺激信号传递至免疫细胞的另一种免疫细胞受体的跨膜结构域的序列；在本发明的一些实施方案中，ESR包含衍生自选自以下的蛋白质的跨膜结构域的序列：TNFRSF1A、TNFRSF1B、TNFRSF3(LTRβ)、TNFRSF6(Fas)、TNFRSF8(CD30)、TNFRSF10A(DR4)、TNFRSF10B(DR5)、TNFRSF19(TROY)、TNFRSF21(DR6)、CTLA4、TNFRSF4(OX40)、TNFRSF5(CD40)、TNFRSF7(CD27)、TNFRSF9(4-1BB)、TNFRSF11A(RANK)、TNFRSF13B(TACI)、TNFRSF13C(BAFF-R)、TNFRSF14(HVEM)、TNFRSF17(CD269)、TNFRSF18(GITR)、CD28、CD28H(TMIGD2)、诱导型T细胞共刺激因子(ICOS/CD278)、DNAX辅助分子1(DNAM-1/CD226)、信号转导淋巴细胞激活分子(SLAM/CD150)、T细胞免疫球蛋白和粘蛋白结构域(TIM-1/HAVcr-1)、干扰素受体α链(IFNAR1)、干扰素受体β链(IFNAR2)、白细胞介素2受体β亚基(IL2RB)、白细胞介素2受体γ亚基(IL2RG)、肿瘤坏死因子超家族14(TNFSF14/LIGHT)、自然杀伤细胞2族成员D(NKG2D/CD314)和CD40配体(CD40L)。优选地，跨膜结构域为人蛋白；在本发明的一些实施方案中，ESR包含多肽，该多肽的序列与选自SEQ ID NO：365-396的序列具有至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％或100％同一性。
在本发明的一些实施方案中，增强型信号传导受体包含与选自SEQ ID NO：274-318的序列具有至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％或100％同一性的序列。这些序列包含N末端分泌信号(例如MLGIWTLLPLVLTSVARLSSKSVNA、MEQRPRGCAAVAAALLLVLLGARAQG、MGLSTVPDLLLPLVLLELLVGIYPSGVIG、MGTSPSSSTALASCSRIARRATATMIAGSLLLLGFLSTTTA、MEQRGQNAPAASGARKRHGPGPREARGARPGPRVPKTLVLVVAAVLLLVSAES和MAVMAPRTLVLLLSGALALTQTWA是用于这些ESRs的信号序列)。信号序列的功能是将ESR转移到膜中。ESR的信号序列被信号肽酶去除，并且不形成最终受体的一部分，因此任何功能性分泌信号都可以被另一功能性分泌信号替代，而不改变ESR的活性。明确考虑了这样的替代。增强型信号传导受体包含与选自SEQ ID NO：274-318的序列的非信号序列部分具有至少90％同一性、或至少95％同一性、或至少96％同一性、或至少97％同一性、或至少98％同一性、或至少99％或100％同一性的序列。
在本发明的一些实施方案中，编码ESR的基因在免疫细胞例如T细胞中表达，并增加免疫细胞的存活或增殖、或T细胞杀伤肿瘤微环境内的细胞的能力。本发明的一个方面是免疫细胞，其基因组包含编码ESR的基因，ESR增加免疫细胞的存活或增殖或T细胞杀伤肿瘤微环境内的细胞的能力。
优选的实施方案包括包含编码ESR的基因的多核苷酸，其中该基因与异源启动子可操作地连接。可与编码ESR的基因可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码ESR的基因的多核苷酸，其中该基因与异源聚腺苷酸化信号可操作地连接，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQID NO：160-217的序列。一些实施方案包括包含编码ESR的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码ESR的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。更优选地，ESR由基因转移多核苷酸编码，该基因转移多核苷酸是piggyBac样转座子的一部分，例如一种还包含如SEQ ID NO：6和7、或SEQ IDNO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列的多核苷酸。ESR可由基因转移多核苷酸编码，该基因转移多核苷酸是Mariner转座子如睡美人转座子的一部分，还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。ESR可由基因转移多核苷酸编码，该基因转移多核苷酸是hAT转座子例如TcBuster转座子的一部分，还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。包含转座子和编码ESR的多核苷酸的基因转移多核苷酸还可包含编码嵌合抗原受体的基因。可以将基因转移多核苷酸与相应的转座酶一起导入免疫细胞，该转座酶可以作为编码转座酶的多核苷酸提供。免疫细胞优选为T细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码ESR的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。
在一些实施方案中，在免疫细胞中表达的第二基因增强ESR的作用以增加免疫细胞的存活或增殖。本发明的一个方面是免疫细胞，其基因组包含编码ESR的基因和增强ESR的活性以增加免疫细胞的存活或增殖的第二基因(ESR增强基因(ESR potentiatinggene))。在一些实施方案中，第二基因与异源启动子可操作地连接；在一些实施方案中，第二基因编码凋亡通路的抑制因子；在一些实施方案中，凋亡通路的抑制因子是半胱天冬酶通路中的显性失活基因，例如半胱天冬酶3(Caspase 3)、半胱天冬酶7、半胱天冬酶8、半胱天冬酶9、半胱天冬酶10或CASP8和FADD样凋亡调节剂(CFLAR)的显性失活突变体；在一些实施方案中，凋亡通路的抑制因子包含选自SEQ ID NO：240-245的序列的显性失活突变体；在一些实施方案中，凋亡通路的抑制因子包含选自SEQ ID NO：237、238或261-272的序列。
优选地，相对于不表达免疫细胞存活增强基因的免疫细胞的半衰期，表达ESR和ESR增强基因的免疫细胞的半衰期增加至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。优选地，相对于不表达免疫细胞存活增强基因的免疫细胞的最大寿命，表达ESR和ESR增强基因的免疫细胞的最大寿命增加至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。优选地，相对于表达ESR和ESR增强基因的免疫细胞的倍增时间，不表达ESR和ESR增强基因的免疫细胞的倍增时间高出至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。优选地，相对于不表达ESR和ESR增强基因的免疫细胞的增殖速率，表达ESR和ESR增强基因的免疫细胞的增殖速率增加至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。
优选的实施方案包括包含编码凋亡抑制因子(inhibitor of apoptosis)的核酸的多核苷酸，其中该核酸与异源启动子可操作地连接。可与编码凋亡抑制因子的基因可操作地连接的示例性异源启动子包括EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子，例如选自SEQID NO：94-154的序列。优选的实施方案包括包含编码凋亡抑制因子的核酸的多核苷酸，其中该核酸与异源聚腺苷酸化信号可操作地连接，例如来自感染哺乳动物细胞的病毒的聚腺苷酸化信号、哺乳动物EF1聚腺苷酸化信号、哺乳动物生长激素聚腺苷酸化信号或哺乳动物珠蛋白聚腺苷酸化信号，例如选自SEQIDNO：160-217的序列。优选的实施方案包括包含编码凋亡抑制因子的基因的多核苷酸，其中该多核苷酸是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。优选的实施方案包括包含编码凋亡抑制因子的基因的多核苷酸，其中该多核苷酸是Mariner转座子如睡美人转座子的一部分，其还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。优选的实施方案包括包含编码凋亡抑制因子的基因的多核苷酸，其中该多核苷酸是hAT转座子如TcBuster转座子的一部分，其还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。可以将包含编码凋亡抑制因子的多核苷酸的piggyBac样转座子与编码相应转座酶的多核苷酸一起导入免疫细胞中。优选的实施方案包括包含编码凋亡抑制因子的基因的多核苷酸，其中该多核苷酸是慢病毒载体的一部分。包含编码凋亡抑制因子的多核苷酸的慢病毒载体可以被包装并用于感染免疫细胞。免疫细胞优选为T细胞或B细胞。
本发明的一个方面是免疫细胞，其基因组包含异源多核苷酸，该异源多核苷酸包含编码凋亡抑制因子的基因。在一些实施方案中，异源多核苷酸包含慢病毒载体、或piggyBac样转座子、或Mariner转座子如睡美人转座子、或hAT转座子如TcBuster转座子。
任选地，包含编码ESR的基因的多核苷酸还包含与异源启动子可操作地连接的编码凋亡抑制因子的第二基因。
5.3.2.1FAS/4-1BB
作为示例性ESR，其中抑制性受体的胞外结构域与共刺激受体的胞内结构域融合，我们设计了一种ESR，其包含TNFRSF6(Fas)的胞外结构域(SEQ ID NO：323)并且还包含TNFRSF6(Fas)的跨膜结构域(SEQ ID NO：387)并且还包含TNFRSF9(4-1BB)的胞内结构域(SEQ ID NO：344)。这种包含序列SEQ ID NO：274的ESR(Fas/4-1BB)是如6.2节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
Fas/4-1BB的活性通过凋亡抑制因子Casp7的显性失活形式Casp7-DN(SEQ ID NO：262)增强。Fas/4-1BB和Casp7-DN增强免疫细胞存活和增殖的作用见6.2.1.2和6.2.2.2节。
本发明的一个方面是包含编码Fas/4-1BB(SEQ ID NO：274)的基因的多核苷酸。本发明的一个方面是基因组包含编码Fas/4-1BB的基因的免疫细胞。本发明的一个方面是包含编码Fas/4-1BB的基因并且还包含编码凋亡抑制因子的基因的多核苷酸；在一些实施方案中，凋亡抑制因子是Casp7的显性失活突变体，例如SEQ ID NO：262。优选地，多核苷酸是转座子。本发明的一个方面是基因组包含编码Fas/4-1BB和显性失活凋亡抑制因子(dominant negative inhibitor of apoptosis)的基因的免疫细胞。这种免疫细胞特别有利于细胞培养中的离体生长。
5.3.2.2抗CD28/OX40是具有增殖增强活性的ESR
作为示例性ESR，其中胞外结构域包含来自与共刺激受体的胞内结构域融合的抗体的结合结构域，我们设计了一种ESR，其胞外结构域包含与TNFRSF4(OX40)的跨膜结构域(SEQ ID NO：373)和TNFRSF4(OX40)的胞内结构域(SEQ ID NO：341)融合的CD28激动剂抗体TGN1412的结合结构域(SEQ ID NO：340)。抗CD28/OX40 ESR的序列如(SEQ ID NO：307)所示。在6.2.2.1节中描述了这种ESR促进T细胞增殖的有效性。
本发明的一个方面是包含编码抗CD28/OX40 ESR(例如SEQ ID NO：34)的基因的多核苷酸。本发明的一个方面是基因组包含编码抗CD28/OX40 ESR的基因的免疫细胞。这种免疫细胞特别有利于细胞培养中的离体生长。
5.4试剂盒
本发明的特征还在于试剂盒，其包含作为蛋白质或由核酸编码的转座酶和/或转座子；或如本文所述的基因转移系统，其包含与转座子组合的作为蛋白质或由如本文所述的核酸编码的转座酶；任选地具有药学上可接受的载体、佐剂或媒介物，并且任选地具有使用说明书。本发明试剂盒的任何组分可以随后的顺序或平行地施用和/或转染到细胞中，例如转座酶蛋白或其编码核酸可以在施用和/或转染转座子之前、同时或之后施用和/或转染到如上定义的细胞中。或者，可以在转染转座酶蛋白或其编码核酸之前、同时或之后，将转座子转染到如上定义的细胞中。如果平行转染，优选地，两种组分以单独的制剂提供和/或在施用前直接彼此混合以避免转染前转座。另外，试剂盒的至少一种组分的施用和/或转染可以以时间交错的方式发生，例如通过施用该组分的多剂量。
6.实施例
以下实施例说明了本文公开的方法、组合物和试剂盒，并且不应被解释为以任何方式限制。从以下实施例中各种等同物将变得明显；这些等同物也被认为是本文公开的本发明的一部分。
6.1用于在免疫细胞中表达的基因转移系统元件
6.1.1人T细胞系中的转座子元件
Jurkat细胞是一种永生化人T细胞系，它们可用于测试基因转移系统在人免疫细胞、特别是T细胞中的有效性。我们测试了爪蟾和家蚕piggyBac样转座酶将它们相应的转座子转座到Jurkat人T细胞系的基因组中的能力。
构建了一种包含爪蟾转座子的多核苷酸(CD19-GFP-LPN1，核苷酸序列如SEQ IDNO：223所示)，其中编码CD19(氨基酸序列如SEQ ID NO：228所示)的核酸与序列如SEQ IDNO：94所示的EF1启动子和如SEQ ID NO：174所示的牛生长激素多腺苷酸化信号序列可操作地连接。CD19基因的一侧侧翼为HS4绝缘子(SEQ ID NO：92)，另一侧侧翼为D4Z4绝缘子(SEQID NO：88)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：ITR 8(其为SEQ ID NO：6的一个实施方案)，紧接着是如SEQ ID NO：1所示的额外转座子末端序列。基因转移多核苷酸在另一绝缘子的远端还包含如SEQ ID NO：4所示的额外转座子末端序列，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：9(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。转座子还包含与CMV启动子和牛生长激素多腺苷酸化信号序列可操作地连接的编码GFP的多核苷酸。CD19和GFP基因放置成使得piggyBac样爪蟾转座子通过其相应的转座酶转座CD19基因，但将GFP基因留在质粒中。
构建了一种包含家蚕转座子的多核苷酸(CD19-RFP-LPN2，核苷酸序列如SEQ IDNO：224所示)，其中编码CD19的基因(SEQ ID NO：228)与序列如SEQ ID NO：94所示的EF1启动子和如SEQ ID NO：174所示的牛生长激素多腺苷酸化信号序列可操作地连接。CD19基因的一侧侧翼为HS4绝缘子(SEQ ID NO：92)，另一侧侧翼为D4Z4绝缘子(SEQ ID NO：88)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：14，紧接着是如SEQ ID NO：12所示的额外转座子末端序列。基因转移多核苷酸在另一绝缘子的远端还包含如SEQ ID NO：13所示的额外转座子末端序列，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：15，紧接着是靶序列5'TTAA-3'。转座子还包含与CMV启动子和牛生长激素多腺苷酸化信号序列可操作地连接的编码RFP的多核苷酸。将CD19和RFP基因放置成使得piggyBac样家蚕转座子通过其相应的转座酶转座CD19基因，但将RFP基因留在质粒中。
根据制造商的说明书，使用Neon电穿孔仪，用1μg CD19-GFP-LPN1质粒DNA和100ng编码序列如EQ ID NO：37所示的爪蟾转座酶的转座酶mRNA转染一份Jurkat细胞样品(每次转染200,000个细胞)。根据制造商的说明书，使用Neon电穿孔仪，用1μg CD19-RFP-LPN2质粒DNA和100ng编码序列如SEQ ID NO：68所示的转座酶的家蚕转座酶mRNA转染两份Jurkat细胞样品(每次转染200,000个细胞)。不同时间后，用抗CD19抗体标记细胞，并通过流式细胞术测定表达CD19的细胞的百分比。结果如表1所示。最初约85％的转染细胞显示出CD19表达(表1第1行)。这对应于已摄入细胞的质粒和已稳定整合到T细胞系基因组中的转座子的表达组合。在接下来的10天内，对于用爪蟾piggyBac样转座子转染的细胞，表达CD19的细胞的百分比降至18％，而对于用家蚕piggyBac样转座子转染的细胞，表达CD19的细胞的百分比降至27％(表1第3行)。这对应于CD19基因尚未整合到基因组中的细胞表达缺失。从转染后约15天直到转染后至少55天，表达CD19的细胞的百分比保持大致恒定(表1第3-6行)。在此期间，细胞一直在生长和分裂。质粒在人体细胞中无法复制，因此细胞保持表达CD19的唯一途径是如果CD19基因整合到基因组中，然后在每次细胞分裂时复制CD19基因以及基因组的其余部分。通过随机片段化的整合率非常低：预计有0.01％到1％的细胞会整合转染的DNA。整合CD19基因的细胞百分比远高于随机整合所预期的频率，但与转座所预期的频率一致。还分析了细胞的GFP和RFP的表达。到第55天，没有可检测的GFP或RFP表达。如上所述，将GFP和RFP基因置于不能被转座酶转座的基因转移质粒的一部分上。因此，如果基因转移质粒已经通过随机片段化和整合而整合到细胞基因组中，可以预期GFP和RFP表达。然而，如果CD19基因由于转座而整合，GFP或RFP基因将留在质粒中并将随时间逐渐降解。高的基因组整合频率和缺乏非转座基因的表达导致我们得出这样的结论：基于爪蟾和家蚕piggyBac样转座子系统的基因转移系统都能够将多核苷酸整合到人T细胞中。
在转染后70天，我们使用荧光激活细胞分选来从不表达CD19的细胞中分选表达CD19的细胞。然后将这些细胞在液体培养物中维持240天以上，并在不同时间分析以评估整合的稳定性。图1显示了转染后155天和FACS分选后85天，y轴上CD19的表达，x轴上荧光蛋白的表达。基本上所有细胞仍然表达CD19，但没有细胞表达荧光蛋白。转染后240天获得了相同的结果。我们得出结论，爪蟾和家蚕piggyBac样转座子即使在没有选择压力的情况下也稳定维持了至少240天。我们还注意到维持表达表明在转座子上编码的基因在超过200代的细胞中没有沉默。因此，基于爪蟾(Xenopus)和家蚕(Bomby)pigyBac样转座子系统的基因转移系统都可用于将用于表达的基因递送到人T细胞中。
6.1.2 T细胞中的启动子元件
6.1.2.1 Jurkat细胞中的启动子测试
Jurkat细胞是一种永生化人T细胞系，它们可用于测试基因转移系统在人免疫细胞、特别是T细胞中的有效性。
通过将启动子元件(任选地与内含子元件组合)导入序列如SEQ ID NO：218所示的第一多核苷酸和序列如SEQ ID NO：219所示的第二多核苷酸之间，将它们克隆到转座子中用于表达人CD19，以产生环状质粒，其包含序列如SEQ ID NO：90所示的绝缘子序列、序列如SEQ ID NO：93所示的绝缘子序列、并且侧翼为一对转座子末端，其中一个包含SEQ ID NO：6的实施方案中的序列SEQ ID NO：8，且一个包含SEQ ID NO：7的实施方案中的序列SEQ IDNO：9。根据制造商的说明书，使用Neon电穿孔仪，用1μg质粒DNA和100ng编码具有氨基酸序列SEQ ID NO：37的爪蟾转座酶的转座酶mRNA转染Jurkat细胞(每次转染200,000个细胞)。
在转染后的不同时间取样进行FACS分析，对其表面表达CD19的活细胞分数进行计数，并示于表2中。表2显示CD19可操作地连接至EF1(例如SEQ ID NO：94和132)和EEF2(例如SEQ ID NO：108)的细胞显示出高初始百分比的CD19表达细胞(D列)，但这不是持续的(例如F和G列)。例如，在第2天和第23天，表达大鼠EF1-驱动的CD19的细胞的百分比从87％降至25％，类似地，在第2天和第23天，表达人EF1-驱动的CD19的细胞的百分比从76％降至37％。相反，当CD19与GAPDH、泛素和PGK启动子可操作地连接时，细胞显示出更加一致的持续表达水平，约50％的细胞在第2天和第23天之间的每个采样时间表达CD19(D-G列)。H列显示了在第2天和第23天之间表达CD19的细胞的百分比下降。
CD19是在细胞表面表达的分子。跨膜蛋白的大量过度表达可能是有毒的。因此，我们推测，表现出表达CD19细胞的最显著损失的启动子可能是那些驱动最强表达的启动子。为了评估启动子强度，我们将每个启动子与编码GFP的基因可操作地连接，并将基因一式三份地转染到HEK细胞中。2天后，在荧光计中测量荧光。平均荧光值示于表2I列中。最强的启动子是EF1和EEF2(I列，1、2和6行)，并且这些是相同的启动子，它们在表达CD19的细胞百分比方面表现出最显著的下降(表2，H列)。相反，PGK、GAPDH和泛素启动子的活性仅为最强EF1启动子的8.6％、28％和22％，但与这些启动子可操作地连接的表达CD19的细胞的百分比是持续的。因此，对于在T细胞中表达编码跨膜蛋白的基因而言，中等活性的启动子似乎优于高活性的启动子，因为它们产生足够高水平的跨膜蛋白以实现功能而不引起毒性。跨膜蛋白包括T细胞受体、嵌合抗原受体和增强型信号传导受体。中度活性启动子包括磷酸甘油酸激酶启动子、甘油醛-3-磷酸脱氢酶启动子和泛素启动子。它们还可以包括已经被减毒的高活性启动子，例如通过去除内含子或启动子的部分缺失，例如减毒EF1启动子或减毒EEF2启动子。
这是意料不到的结果：目前在表达嵌合抗原受体方面的大部分工作是用强活性启动子如CMV或EF1启动子进行的。相反，本文中我们已经发现这样的高活性启动子在可操作地连接到跨膜蛋白时是不利的。用于在T细胞中表达编码跨膜蛋白的基因的有利基因转移系统包含多核苷酸，该多核苷酸包含与选自磷酸甘油酸激酶启动子、甘油醛-3-磷酸脱氢酶启动子、泛素启动子、减毒EF1启动子或减毒EEF2启动子的启动子可操作地连接的编码跨膜蛋白的基因。示例性磷酸甘油酸激酶启动子序列如SEQ ID NO：115-118所示。例示性甘油醛-3-磷酸脱氢酶启动子序列如SEQ ID NO：97-107所示。示例性泛素启动子序列如SEQ IDNO：95和125-127所示。多核苷酸还可以包含选自SEQ ID NO：87-93的绝缘子序列。优选地，基因转移多核苷酸包含转座子末端，使得其被相应的转座酶识别和转座，使得这种转座可以将启动子及其可操作地连接的基因插入到免疫细胞例如T细胞的基因组中。多核苷酸可以是piggyBac样转座子的一部分，piggyBac样转座子还包含如SEQ ID NO：6和7、或SEQ IDNO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21所示的序列。多核苷酸可以是Mariner转座子如睡美人转座子的一部分，Mariner转座子还包含与SEQ ID NO：24具有90％同一性的序列和与SEQ ID NO：25具有90％同一性的序列。多核苷酸可以是hAT转座子例如TcBuster转座子的一部分，hAT转座子还包含与SEQ ID NO：397具有90％同一性的序列和与SEQ ID NO：398具有90％同一性的序列。重复进行实验，8天后用抗CD19抗体标记细胞，并通过流式细胞术测量第8天表达CD19的细胞的平均荧光强度。平均荧光强度值示于表3的D列中。为了进行比较，对每个细胞以大约22,000个分子自然表达CD19的人类B细胞进行了标记，并测量了平均荧光强度。如表3的E列中所示，使用B细胞的平均荧光强度来计算在Jurkat细胞表面上表达的CD19分子的数量。对于所有测试的启动子，每个细胞表面上CD19分子的数量是在B细胞上天然发现的数量的2-5倍。用可操作地连接至EF1启动子的CD19转染的细胞的平均荧光强度在用可操作地连接至PGK启动子的CD19转染的细胞的值的20％内(比较表3第5行和第6行)，即使已知PGK启动子的活性远低于EF1启动子，例如如表2第I列第5和第6行所示。我们认为这是因为其中CD19的表达超过B细胞表面正常数量约5倍的细胞会经历毒性。EF1是一种更强的启动子，因此用与EF1启动子可操作地连接的CD19转染的细胞中有更高细胞超过该毒性极限并死亡。这导致在表2第6行的第2天和第8天之间观察到表达CD19的细胞的损失。因此，中度活性启动子能够产生高水平的跨膜蛋白表达，但该水平不太可能高到产生毒性。这在用跨膜蛋白如嵌合抗原受体转染T细胞中是有利的。
表3显示序列如SEQID NO：94、95、98、108、115和132所示的启动子都有效驱动了Jurkat永生化T细胞中高水平的CD19表达。用于在T细胞中表达基因的有利的基因转移系统包含多核苷酸，该多核苷酸包含具有选自SEQ ID NO：94、95、98、108、115和132的序列的启动子。用于在T细胞中表达基因的有利的基因转移系统包含多核苷酸，该多核苷酸包含选自SEQ ID NO：87-91的绝缘子序列和选自SEQ ID NO：92和93的绝缘子序列。用于在T细胞中表达基因的有利的基因转移系统包含多核苷酸，该多核苷酸包含含有序列SEQ ID NO：6的转座子末端和含有序列SEQ ID NO：7的转座子末端。
6.1.2.2原代T细胞中的启动子测试
通过将启动子元件导入序列如SEQ ID NO：220所示的第一多核苷酸和序列如SEQID NO：221所示的第二多核苷酸之间，将它们克隆到用于表达人CD19的转座子中，以产生环状质粒，其包含序列如SEQ ID NO：88所示的绝缘子序列、序列如SEQ ID NO：92所示的绝缘子序列、并且侧翼为一对转座子末端，其中一个包含靶位点5'-TTAA-3'、紧接着序列SEQ IDNO：8、紧接着序列SEQ ID NO：1，另一个包含序列SEQ ID NO：4、紧接着序列SEQ ID NO：9、紧接着靶位点5'-TTAA-3'。根据制造商的说明书，使用Neon电穿孔仪，用1μg质粒DNA和100ng编码具有多肽序列SEQ ID NO：37的爪蟾转座酶的转座酶mRNA转染原代T细胞(每次转染200,000个细胞)。11天后，用抗CD19抗体标记细胞并通过流式细胞术测量平均荧光强度。
表4显示序列如SEQ ID NO：97、98和108-114所示的启动子均有效驱动了原代T细胞中高水平的CD19表达。它进一步显示通过使用不同的启动子可以获得不同的表达水平。用于在T细胞中表达基因的有利的基因转移系统包含多核苷酸，该多核苷酸包含具有选自SEQ ID NO：97、98和108-114的序列的启动子。用于在T细胞中表达基因的有利的基因转移系统包含多核苷酸，该多核苷酸包含含有序列SEQ ID NO：8、紧接着是序列SEQ ID NO：1的转座子末端，以及含有序列SEQ ID NO：4、紧接着是SEQ ID NO：9的转座子末端。
6.2用于增强免疫细胞的存活和功效的元件
本发明的一个方面是公开了可用于增强免疫细胞存活、增殖或扩增的序列。
细胞存活可以测量为群体中只有一半细胞保持存活所用的时间长度(半衰期)，或群体中所有细胞死亡所用的时间(最大寿命)。表达免疫细胞存活增强基因的免疫细胞比不表达免疫细胞存活增强基因的免疫细胞存活更长。测量这种作用的一种方式是将异源多核苷酸整合到免疫细胞的基因组中，其中异源多核苷酸包含与调控序列可操作地连接的免疫细胞存活增强基因，该调控序列使其在免疫细胞内表达，换言之，对于在免疫细胞中表达是有效的。异源多核苷酸还包含编码选择标记的基因，例如可以容易地鉴定的基因，如荧光蛋白或细胞表面蛋白。基因组包含异源多核苷酸的细胞表达免疫细胞存活增强基因，并且它们可以通过选择性标记的存在来识别。存活的增强可以测量为相对于不表达免疫细胞存活增强基因的免疫细胞，表达免疫细胞存活增强基因的免疫细胞的半衰期的增加。相对于不表达免疫细胞存活增强基因的免疫细胞的半衰期，表达免疫细胞存活增强基因的免疫细胞的半衰期增加至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。这种增加可以通过比较具有和不具有存活增强基因的特定免疫细胞的相等大小群体的存活率来测量。相对于不表达免疫细胞存活增强基因的免疫细胞的最大寿命，表达免疫细胞存活增强基因的免疫细胞的最大寿命增加至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。最大寿命的百分比变化可以通过比较具有和不具有存活增强基因的特定免疫细胞的相等大小的群体来测量。
细胞增殖可以测量为群体中细胞数量加倍所需的时间长度(倍增时间)，或测量为单位时间长度内细胞群体增加的分数(增殖速率)。表达免疫细胞增殖增强基因的免疫细胞可以分裂更长时间，或者它们可以比不表达免疫细胞增殖增强基因的免疫细胞分裂得更快。测量这种作用的一种方式是将异源多核苷酸整合到免疫细胞的基因组中，其中异源多核苷酸包含与调控序列可操作地连接的免疫细胞增殖增强基因，该调控序列使其在免疫细胞内表达。异源多核苷酸还包含编码选择标记的基因，例如可以容易地鉴定的基因，如荧光蛋白或细胞表面蛋白。基因组包含异源多核苷酸的细胞表达免疫细胞增殖增强基因，并且它们可以通过选择性标记的存在来识别。增殖的增强可以测量为相对于不表达免疫细胞增殖增强基因的免疫细胞，表达免疫细胞增殖增强基因的免疫细胞的倍增时间的减少。相对于表达免疫细胞增殖增强基因的免疫细胞的倍增时间，不表达免疫细胞增殖增强基因的免疫细胞的倍增时间高出至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。相对于不表达免疫细胞增殖增强基因的免疫细胞的增殖速率，表达免疫细胞增殖增强基因的免疫细胞的增殖速率增加加至少5％、或至少10％、或至少15％、或至少20％、或至少25％、或至少30％、或至少35％、或至少40％、或至少45％、或至少50％、或至少55％、或至少60％、或至少65％、或至少70％、或至少75％、或至少80％、或至少85％、或至少90％、或至少95％、或至少100％。增殖速率或倍增时间可以在免疫细胞已经开始表达免疫细胞增殖增强基因后的不同时间进行测量。在异源多核苷酸整合到免疫细胞的基因组中5天后、或10天后、或15天后、或20天后、或25天后后、或30天后、或35天后、或40天后、或45天后、或50天后、或55天后、或60天后、或在免疫细胞开始表达免疫细胞增殖增强基因后，表达免疫细胞增殖增强基因的免疫细胞的增殖速率可相对于不表达免疫细胞增殖增强基因的相同免疫细胞的增殖速率增加。
本发明的另一个方面是公开了可用于增加免疫细胞在降低正常免疫细胞效力的条件下保持有效的时间长度的序列。正常T细胞在反复暴露于抗原时经历细胞凋亡(“再刺激-诱导的细胞死亡”)，并且那些没有死亡的细胞无法杀伤表达抗原的细胞(Voss等人，2002)。(2017)Cancer Lett.408：190-196.“代谢重编程和细胞凋亡敏感性：定义T细胞应答的轮廓”)。尽管这有助于降低自身免疫性，但它是防止T细胞有效对抗实体瘤的影响因素。因此，在这些情况下，期望在重复抗原暴露期间保持免疫细胞功能并防止再刺激诱导的细胞死亡。再刺激诱导的细胞死亡可以通过计数暴露于抗原(例如肿瘤细胞上的抗原)2、3、4或更多次后存活的T细胞的数量来测量。当两个群体具有相同的抗原暴露程度和频率时，异源表达的序列防止再刺激诱导的细胞死亡的能力可以通过比较表达该序列的T细胞的存活与不表达该序列的T细胞的存活来测量。存活的增强可以测量为相对于重复暴露于抗原时不表达免疫细胞存活增强基因的免疫细胞，表达免疫细胞存活增强基因的剩余免疫细胞的数量的增加。相对于在重复暴露于例如在肿瘤细胞中表达的抗原时不表达免疫细胞存活增强基因的存活免疫细胞的数量，表达免疫细胞存活增强基因的存活免疫细胞的数量增加至少10％、或至少20％、或至少30％、或至少40％、或至少50％、或至少60％、或至少70％、或至少80％、或至少90％、或至少100％、或至少150％、或至少200％、或至少250％、或至少300％、或至少350％、或至少400％、或至少450％、或至少500％。
对再刺激诱导的细胞死亡的抗性和持续的免疫细胞效力可以通过计数T细胞在暴露于肿瘤细胞2、3、4或更多次后杀伤细胞如肿瘤细胞的能力来测量。当两个群体具有相同的抗原暴露程度和频率时，异源表达的序列维持免疫细胞功能的能力可以通过比较表达该序列的T细胞的细胞杀伤活性与不表达该序列的T细胞的细胞杀伤活性来测量。相对于重复暴露于肿瘤细胞时不表达T细胞效力增强基因的存活免疫细胞的数量，表达T细胞效力增强基因的T细胞的细胞杀伤活性增加至少10％、或至少20％、或至少30％、或至少40％、或至少50％、或至少60％、或至少70％、或至少80％、或至少90％、或至少100％、或至少150％、或至少200％、或至少250％、或至少300％、或至少350％、或至少400％、或至少450％、或至少500％。
6.2.1T细胞转化元件
6.2.1.1突变STAT3在原代T淋巴细胞中的表达
将编码STAT3的突变形式(STAT3-Y640F)的基因与序列如SEQ ID NO：115所示的PGK启动子和序列如SEQ ID NO：182所示的兔珠蛋白聚腺苷酸化信号可操作地连接，并克隆到基因转移多核苷酸中。该基因转移多核苷酸还包含GFP报告基因(序列如SEQ ID NO：222所示)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列如SEQ ID NO：92所示)，另一侧侧翼为D4Z4绝缘子(序列如SEQ ID NO：88所示)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是额外转座子末端序列SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)。基因转移多核苷酸在另一绝缘子的远端还包含额外转座子末端序列SEQ ID NO：5(其与SEQ ID NO：4具有>95％的同一性)，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自正常供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码具有多肽序列SEQ ID NO：37的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。在转染后的不同时间取样，用荧光标记的抗CD8抗体孵育，并在荧光激活细胞分选仪(FACS)上分析CD8和Dasher GFP。
图2显示了细胞染色随时间的分布。CD8染色用作CD8+ T细胞的标记，并显示在图A中显示的每个FACS图的y轴上。GFP荧光显示在每个FACS图的x轴上；GFP荧光指示细胞正在表达GFP，并且本文也用作指示细胞内存在基因转移多核苷酸的标志物。在第14天，大约97.8％的细胞显示出强CD8染色(即它们位于FACS图的上半部)，且大约9.8％的被分析细胞都是CD8+并显示GFP荧光。表达CD8并显示GFP荧光的细胞分数随时间增加：第28天增加23.9％，第34天增加41.1％，第41天增加62.4％，第48天增加79.3％。表达GFP的T细胞群体分数的增加表明基因组包含基因转移多核苷酸的T细胞与基因组不包含基因转移多核苷酸的T细胞相比具有存活优势，或者表明基因组包含基因转移多核苷酸的T细胞与基因组不包含基因转移多核苷酸的T细胞相比具有增殖优势。这种存活或增殖优势并非源于GFP的表达(我们看到了许多GFP表达与存活或增殖优势无关的实例)，而是源于STAT3-Y640F的表达。我们得出结论，STAT3-Y640F在T细胞中的表达为它们提供了存活或增殖优势，并且编码STAT3的激活突变体的基因是如第5.3.1.1节中描述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
6.2.1.2原代T细胞中T细胞转化元件和ESRs的表达
将编码一组T细胞转化元件和增强型信号传导受体的基因单独克隆到单独的基因转移多核苷酸中。在每种情况下，将该基因与序列如SEQ ID NO：115所示的PGK启动子和序列如SEQ ID NO：182所示的兔珠蛋白多腺苷酸化信号可操作地连接，并克隆到基因转移多核苷酸中。该基因转移多核苷酸还包含GFP报告基因(序列如SEQ ID NO：222所示)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列如SEQID NO：92所示)，另一侧侧翼为D4Z4绝缘子(序列如SEQ ID NO：88所示)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是额外转座子末端序列SEQID NO：3(其与SEQ ID NO：1具有>95％同一性)。基因转移多核苷酸在另一绝缘子的远端还包含额外转座子末端序列SEQ ID NO：5(其与SEQ ID NO：4具有>95％的同一性)，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自正常供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书步骤，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码具有多肽序列SEQ ID NO：37的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。在转染后的24天取样，用荧光标记的抗CD8抗体孵育，并在荧光激活细胞分选仪(FACS)上分析CD8和Dasher GFP。数据示于表5中。
如6.2.1.1节所述，表达GFP的CD8+细胞的富集是基因转移多核苷酸包含赋予T细胞存活或增殖优势的基因的指示物，也如5.3.1.1节所述。在这组基因转移多核苷酸中，CD19作为细胞表面标志物被包括在内，预计对T细胞存活没有影响，因此我们使用了在用CD19转染的细胞中表达GFP的细胞的百分比(3％，参见表5第10行)作为对推定的存活增强基因进行基准测试的水平。用具有两个激活突变D124E和T195P的编码CD28的基因转染的T细胞比用CD19转染的细胞多10倍以上的表达GFP的细胞(表5第1行)。编码识别与CD3e或CD3d融合的表皮生长因子受体(EGFR)的抗体片段的基因似乎使表达GFP细胞的百分比比CD19增加4倍(分别为表5的第2行和第3行)，尽管我们注意到CD3d融合的第二次测量显示GFP表达的百分比低得多(表5的第15行)。与CD19相比，天然生存素基因似乎使GFP表达增加3倍(表5第4和5行)。还示出了两个ESRs。首先，包含与CD28跨膜结构域(序列如SEQ ID NO：395所示)和包含T195P激活突变的CD28胞内结构域(序列如SEQ ID NO：352所示)融合的抗CD28抗体(序列如SEQ ID NO：340所示)导致表达GFP的细胞的数量增加约2倍(表5第6行和第7行)。包含TNFRSF1A胞外结构域(序列如SEQ ID NO：330所示)和跨膜结构域(序列如SEQID NO：394所示)以及4-1BB胞内结构域(序列如SEQ ID NO：344所示)的第二ESR导致表达GFP的细胞的数量增加略小于2倍(表5第8行和第9行)。两种共转染在增加表达GFP的细胞的百分比方面尤其有活性。表5第16行所示的一种共转染包括编码ESR(也描述于5.3.2.1节)的第一基因，该ESR包括Fas受体的胞外结构域和跨膜结构域(TNFRSF6)(序列分别如SEQ IDNO：323和387所示)和4-1BB的胞内结构域(TNFRSF9)(序列如SEQ ID NO：344所示)，以及编码半胱天冬酶7的显性失活突变体的第二基因(序列如SEQ ID NO：262所示)表5第17行所示的第二种共转染包含编码STA3-Y640F的第一基因(序列如SEQ ID NO：246所示)和编码PIK3CA-L1001P的第二基因(序列如SEQ ID NO：257所示)。24天后，这些共转染分别产生51％和46％的表达GFP的细胞。
我们得出结论：T细胞中CD28-D124E-T195P的表达、或ESR Fas/4-1BB和Casp7-DN的共同表达，或STAT3-Y640F和PIK3CA-L1001P共同表达为它们提供了存活或增殖优势，并且这些基因或基因组合是如第5.3.1.1节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
6.2.1.3生存素和CD28的激活突变体增强T细胞功能
如6.2.1.2节和表5所示，我们发现生存素或D124E/T195P激活的CD28双突变体的表达增强了T细胞生长/存活和/或增殖。为了测试这些基因是否也可以增强T细胞性能，我们将它们与靶向CD19的嵌合抗原受体(仅在B细胞上天然发现的表位)一起整合到T细胞的基因组中，并测试了经修饰的T细胞杀伤转化的B细胞系的细胞的能力。
构建了三种基因转移多核苷酸：各自包含GFP报告基因(序列SEQ ID NO：222)，该GFP报告基因包含可操作地连接甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。GFP基因的一侧侧翼为HS4绝缘子(序列SEQID NO：92)，另一侧侧翼为D4Z4绝缘子(序列SEQ ID NO：88)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ IDNO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是日SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)所示的额外转座子末端序列。基因转移多核苷酸在另一绝缘子的远端还包含如SEQ ID NO：5(其与SEQ ID NO：4具有>95％的同一性)所示的额外转座子末端序列，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。该基因转移多核苷酸还包含编码CD10结合嵌合抗原受体(序列如SEQ ID NO：229所示的多肽)的基因，其可操作地连接至PGK启动子或GAPDH启动子：如6.1.2节所述和表3所示，在T细胞中显示相当活性的启动子。嵌合抗原受体基因存在于基因转移多核苷酸中，使得其与所述Dasher GFP基因分散地转录，并且使其处于可被转座酶转座的部分基因转移多核苷酸中。第一基因转移多核苷酸(346463，序列如SEQ ID NO：225所示)不包含另外的转座基因。第二基因转移多核苷酸(346776，序列如SEQ ID NO：226所示)还包含可操作地连接至PGK启动子的编码生存素的开放阅读框，以与嵌合抗原受体相同的方向转录，并且也可在由转座酶转座的部分基因转移多核苷酸中转录。第三基因转移多核苷酸(346777，序列如SEQ ID NO：227所示)包含嵌合抗原受体并且还包含可操作地连接至PGK启动子的编码CD28-D124E-T195P的开放阅读框，以与嵌合抗原受体相同的方向转录，并且也可在由转座酶转座的部分基因转移多核苷酸中转录。
6.2.1.3a生存素和激活的CD28增强的离体CAR细胞杀伤测试1
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自2个不同的正常供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书步骤，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码如SEQ ID NO：37所示的转座酶的mRNA转染约200,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。细胞在培养物中生长约5周，此时约10％的用每种基因转移多核苷酸转染的细胞表达GFP。然后将T细胞样品(200,000)与等量的JY细胞混合：JY是Epstein-Barr病毒永生化B细胞淋巴母细胞样细胞系，其表达CD19并因此是抗CD19嵌合抗原受体的靶标。用抗CD8和抗CD19抗体对混合3天和7天后从细胞混合物中取出细胞样品进行染色(以分别标记T细胞和JY细胞)。结果如图3和表6所示。混合后第3天，仅表达嵌合抗原受体的T细胞基本上消失，被JY肿瘤细胞淹没：仅8％的可检测细胞表达CD8，而89％表达CD19(图3图A，表6的A列第3行和第4行)。混合后7天，仅2.3％的细胞是表达CD8的T细胞(图3的图D，表6的A列第5行和第6行)。相反，表达嵌合受体和生存素或CD28-D124E-T195P的T细胞能够在JY肿瘤细胞存在下存活。如图3的图B和C以及表6的B和C列、第3和4行所示，3天后，40-50％的细胞表达CD8(T细胞标志物)，仅23-29％表达CD19(肿瘤细胞标志物)。到第7天，肿瘤细胞已经被有效地清除，所有细胞中大约90％表达CD8(图3的图E和F以及表6的B和C列、第5和6行)。我们的结论是，生存素或CD28的D124E/T195P激活的双突变体的表达不仅增强T细胞的生长/存活和/或增殖，它们还可以通过使T细胞在肿瘤细胞存在下存活并保持活性以杀伤肿瘤细胞来增强T细胞性能。
6.2.1.3b生存素和激活的CD28增强的离体CAR细胞杀伤
使用FACS分选转染的T细胞的第二份样品以选择表达GFP的细胞(其为转座子在T细胞基因组中存在的指示物)。将所选择的细胞在培养物中再生长一周并测试其在体内杀伤JY肿瘤细胞的能力。通过腹腔注射向NSG免疫功能低下的小鼠施用100万个JY细胞。7天后，通过腹腔注射向JY处理的小鼠施用100万个表达GFP的T细胞。两只小鼠接受磷酸盐缓冲盐水(PBS)代替T细胞的非活性对照处理。如表7所示，接受PBS的小鼠在JY细胞注射后存活了24或25天(表7第1行和第2行)。施用表达嵌合抗原受体的T细胞可将JY注射后的存活期延长5-6天至30天(表7第3行)。施用表达嵌合抗原受体和生存素或CD28-D124E-T195P的T细胞可将JY注射后的存活期延长另外4天至34天(表7第4和5行)。我们的结论是，生存素或CD28的D124E/T195P激活的双突变体的表达不仅增强T细胞的离体生长/存活和/或增殖，它们还可以通过使T细胞在肿瘤细胞存在下存活并保持活性以杀伤肿瘤细胞来增强体内的T细胞性能。
6.2.1.3c生存素和激活的CD28增强的离体CAR细胞杀伤测试2
我们还对仅表达抗CD19嵌合抗原受体或与生存素或CD28-D124E-T195P共表达的嵌合抗原受体的T细胞进行了肿瘤再激发测试(tumor re-challenge test)。由于相对较短的共培养时间和较高的T细胞与肿瘤的比率，标准的单激发离体肿瘤裂解试验(standardsingle challenge ex-vivo tumor-lysis assay)通常高估了T细胞的真正抗肿瘤潜力。为了确定存活增强基因(在这种情况下是生存素和CD28-D124E-T195P)是否也可以增强T细胞功能，我们使用了递归高肿瘤细胞负荷激发以更好地模拟周围的肿瘤微环境来激发T细胞的存活。在总体积为200μl的微滴定板孔中，用1、2、3、4或5个连续剂量的100,000NALM6(其为CD19+、CD20-、CD21-)细胞激发T细胞(100,000)。每100,000个细胞NALM6剂量间隔48小时。对于每次再激发，取出100μl的上清液，并且添加100μl的含有100,000个NALM6细胞的新鲜培养基。在对样品的最后一次激发后24小时，通过添加D荧光素并使用BioTek SynergyNeo2混合酶标仪根据制造商的说明书测量发光，将NALM6细胞死亡测量为生物发光的减少(参见例如Karimi et.al.,(2014)“通过生物发光成像测量细胞毒性优于标准铬-51释放测定”PLoS ONE 9(2)：e89357)。
将用转座子转染的细胞离体培养10个月，该转座子包含抗CD19CAR和任选的编码生存素的基因或编码CD28中激活突变的基因，如6.2.1.3a节所述。此时细胞表达了>95％的GFP(并且推断也表达了CAR，如果存在，还表达了存活增强基因)。T细胞在离体培养中存活10个月是不寻常的。对于表达存活基因的T细胞，我们将这种长寿归因于生存素或CD28-D124E-T195P的表达。然而，我们还观察到仅表达嵌合抗原受体的T细胞的这种长期存活，尽管它们比也表达存活基因的细胞生长得更慢。当编码嵌合抗原受体的基因与PGK启动子或GAPDH启动子或驱动相当表达水平的启动子可操作地连接时，我们将这归因于最佳CAR水平的表达。在长期离体培养损害了T细胞杀伤肿瘤细胞的能力的情况下，我们将6.2.1.3a节中描述的转染重复到来自不同供体的T细胞中，并将细胞离体培养4个月，然后用肿瘤再激发试验测试它们。NALM6细胞死亡结果示于表8中。
表8第1行显示了T细胞用NALM6细胞激发的次数。表8第2-4行显示了离体生长10个月的表达抗CD19嵌合抗原受体的T细胞群对NALM6的杀伤作用。T细胞也表达生存素(第3行)或CD28-D124E-T195P(第4行)。仅表达嵌合抗原受体的细胞在第一次激发时杀伤了100％的NALM6细胞，但在随后的激发时杀伤效率下降：第二次激发后85％、第三次激发后47％、第四次激发后23％以及第五次激发后仅10％的NALM6细胞被杀伤(参见表8第2行)。相比之下，也表达生存素的细胞能够在第五次激发时杀伤76％的NALM6细胞(表8第3行)，并且也表达CD28-D124E-T195P的细胞能够在第五次激发时杀伤82％的NALM6细胞(表8第4行)。在仅离体培养4个月的细胞中观察到相似的杀伤模式，尽管杀伤效率通常更高(表8第5-7行)。仅表达嵌合抗原受体的细胞在第一次和第二次激发时杀伤100％的NALM6细胞，但在随后的激发时杀伤效率下降：在第三次激发后51％、在第四次激发后28％以及在第五次激发后27％的NALM6细胞被杀伤(参见表8第5行)。相比之下，也表达生存素的细胞能够在第五次激发时杀伤90％的NALM6细胞(表8第6行)，并且也表达CD28-D124E-T195P的细胞能够在第五次激发时杀伤91％的NALM6细胞(表8第7行)。
这表明表达嵌合抗原受体的T细胞对生存素或CD28-D124E-T195P的表达增强了这些细胞对靶细胞杀伤并降低了细胞耗尽的速率。用于杀伤肿瘤细胞的有利T细胞包含异源多核苷酸，该异源多核苷酸包含可表达的生存素或CD28-D124E-T195P基因。
6.2.1.4原代T细胞中Bcl2和Bcl6的表达
由病毒CHYSL(2A)序列分离的编码Bcl2和Bcl6的开放阅读框(完整开放阅读框Bcl2-2A-Bcl6的序列如SEQ ID NO：272所示)与序列如SEQ ID NO：115所示的PGK启动子和序列如SEQ ID NO：182所示的兔珠蛋白聚腺苷酸化信号可操作地连接，并克隆到基因转移多核苷酸中。该基因转移多核苷酸还包含GFP报告基因(序列SEQ ID NO：222)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(即，两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列SEQ IDNO：92)，另一侧侧翼为D4Z4绝缘子(序列SEQ ID NO：88)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ IDNO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是如SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)所示的额外转座子末端序列。基因转移多核苷酸在另一绝缘子的远端还包含如SEQ ID NO：5(其与SEQ ID NO：4具有>95％的同一性)所示的额外转座子末端序列，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自正常供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书步骤，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。在转染后的不同时间取样，用荧光标记的抗CD8抗体孵育，并在荧光激活细胞分选仪(FACS)上分析CD8和Dasher GFP。
图4显示了细胞染色随时间的分布。CD8染色用作CD8+ T细胞的标记，并显示在图A中显示的每个FACS图的y轴上。GFP荧光显示在每个FACS图的x轴上；GFP荧光指示细胞正在表达GFP，它在本文中也用作指示细胞内存在基因转移多核苷酸的标志物。图B是显示也表达GFP的表达CD8的T细胞的百分比的图。在转染后第一天，约26％的表达CD8的细胞也表达GFP。到第10天，88.7％的细胞显示强的CD8染色但没有GFP表达，11.3％的表达CD8的细胞也表达GFP，表明它们也含有基因转移多核苷酸。也表现出GFP荧光的表达CD8细胞分数随着时间的推移而增加：在第19天增加29.4％，在第42天增加80％。表达GFP的T细胞群体分数的增加表明基因组包含基因转移多核苷酸的T细胞与基因组不包含基因转移多核苷酸的T细胞相比具有存活优势，或者表明基因组包含基因转移多核苷酸的T细胞与基因组不包含基因转移多核苷酸的T细胞相比具有增殖优势。我们得出结论，Bcl2和Bcl6在T细胞中的表达为它们提供了存活或增殖优势，并且编码Bcl2和Bcl6的基因是如第5.3.1.1节所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
6.2.1.5原代T细胞中T细胞转化元件和ESRs的表达
将编码一组T细胞转化元件和增强型信号传导受体的基因单独克隆到单独的基因转移多核苷酸中。在每种情况下，将该基因与序列如SEQ ID NO：115所示的PGK启动子和序列如SEQ ID NO：182所示的兔珠蛋白多腺苷酸化信号可操作地连接。该基因转移多核苷酸还包含GFP报告基因(序列SEQ ID NO：222)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(即，两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列SEQ ID NO：92)，另一侧侧翼为D4Z4绝缘子(序列SEQ ID NO：88)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是额外转座子末端序列SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)。基因转移多核苷酸在另一绝缘子的远端还包含额外转座子末端序列SEQ ID NO：5(其与SEQID NO：4具有>95％的同一性)，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自2个供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。在转染后的不同时间取样，用荧光标记的抗CD8抗体孵育，并在荧光激活细胞分选仪(FACS)上分析CD8和Dasher GFP。数据示于表9中。
如6.2.1.1节所述，表达GFP的CD8+细胞的富集是基因转移多核苷酸包含赋予T细胞存活或增殖优势的基因的指示物，如5.3.1.1节所述。在这组基因转移多核苷酸中，HSV-TK作为对照基因被包括在内，预计对T细胞存活没有影响。因此，因此我们使用了在用HSV-TK转染的细胞中表达GFP的细胞的百分比作为对推定的存活增强基因进行基准测试的水平。如表9中所见，来自2个供体的T细胞的两次独立转染在7.5％至15.3％的细胞(表9第7行和第8行)中产生初始GFP表达(指示转染效率)。到第14天，这些百分比显著下降，并且在随后的时间，表达GFP的细胞的百分比保持大致稳定或下降。这表明，正如所预期的，HSV-TK不提供具有生长或增殖优势的T细胞。相比之下，包含编码STAT3突变体(STAT3-D661Y和STAT3-S614R-Y640F)的基因的两种测试基因转移多核苷酸显示在2个供体中表达GFP的细胞百分比逐渐增加(表9第1行和第3行)，表明这些基因确实为T细胞提供了生长或增殖优势，类似于6.2.1.1节中针对STA3-Y640F看到的优势。我们得出结论，包括STAT3-D661Y和STA3-S614R-Y640F的激活STAT3突变体在T细胞中的表达为它们提供了存活或增殖优势，并且编码STAT3的激活突变体的基因是如5.3.1.1节中描述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
测试的基因转移多核苷酸之一包含编码凋亡抑制因子Bcl-XL的基因。这些细胞在2个供体中表达GFP的细胞的百分比逐渐增加(表9第2行)，表明Bcl-XL的表达提供具有生长或增殖优势的T细胞，并且编码Bcl-XL的基因是如5.3.1.1节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
测试的基因转移多核苷酸之一包含编码磷脂酶C激活突变(PLCG1-S345F)的基因。这些细胞在2个供体中表达GFP的细胞的百分比增加(表9第6行)，表明PLCG1-S345F的表达为T细胞提供了生长或增殖优势，并且编码PLCG1-S345F的基因是如5.3.1.1节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
在该实验中还测试了两种ESRs。一种TNFR1/CD27(序列如SEQ ID NO：301所示)包含来自TNFRSF1A的胞外结构域(序列如SEQ ID NO：330所示)、来自TNFRSF1A的跨膜结构域(序列如SEQ ID NO：394所示)和来自CD27的胞内结构域(序列如SEQ ID NO：343所示)。第二种TNFR1/4-1BB(序列如SEQ ID NO：302所示)也包含来自TNFRSF1A的胞外结构域(序列如SEQ ID NO：330所示)和来自TNFRSF1A的跨膜结构域(序列如SEQ ID NO：394所示)，在这种情况下与来自4-1BB的胞内结构域(序列如SEQ ID NO：344所示)融合。ESR TNFR1/CD27和ESR TNFR1/4-1BB在其中一个供体中均导致高百分比的表达GFP的细胞(表9第4行)，表明ESR TNFR1/CD27或ESR TNFR1/4-1BB的表达可以为T细胞提供生长或增殖优势，并且编码ESR TNFR1/CD27或ESR TNFR1/4-1BB的基因是如5.3.1.1节中所述的免疫细胞存活增强基因和免疫细胞增殖增强基因。
6.2.1.6 Bcl-XL对原代T细胞杀伤肿瘤细胞的影响
6.2.1.6a Bcl-XL增强的离体BiTE细胞杀伤测试
将编码Bcl-XL(多肽序列如SEQ ID NO：238所示)的基因克隆到基因转移多核苷酸中。将该基因与序列如SEQ ID NO：115所示的PGK启动子和序列如SEQ ID NO：182所示的兔珠蛋白多腺苷酸化信号可操作地连接。该基因转移多核苷酸还包含GFP报告基因(序列SEQID NO：222)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(即，两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列如SEQ ID NO：92所示)，另一侧侧翼为D4Z4绝缘子(序列如SEQ ID NO：88所示)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是额外转座子末端序列SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)。基因转移多核苷酸在另一绝缘子的远端还包含额外转座子末端序列SEQ ID NO：5(其与SEQ IDNO：4具有>95％的同一性)，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自3个供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书步骤，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。
细胞在T细胞培养基中培养生长240天。如6.2.1.6节和表9中所述，Bcl-XL为T细胞提供了选择优势。我们能够培养这些细胞8个月证明了Bcl-XL基因在T细胞中的表达增强了它们的离体存活。除了存活之外，我们测试了这些T细胞是否通过将它们与肿瘤细胞系混合而保留了它们的细胞毒性。在测试细胞毒性之前，我们通过测量GFP来确定表达Bcl_Xl的细胞分数，GFP由整合到Bcl_Xl细胞基因组中的相同转座子表达。图5显示了转染后240天来自3个不同供体的T细胞的流式细胞分析，x轴上为GFP，y轴上为T细胞标志物CD8的染色。图A显示了超过90％的来自供体81的T细胞表达GFP，图B显示了超过99％的来自供体82的T细胞表达GFP，图C显示了超过98％的来自供体84的T细胞表达GFP。因此在240天后，来自每个供体的绝大多数T细胞表达GFP，并且根据推断Bcl-XL。
为了测量细胞毒性，我们将100,000个T细胞与的100,000个B细胞肿瘤系NALM6(其为CD19+、CD20-、CD21-)细胞混合，NALM6含有基因组整合的编码荧光素酶的基因。在一些反应中包括具有针对CD3(在T细胞表面上)和CD19(在NALM6表面上)的结合结构域的双特异性T细胞衔接器(BiTE)，以将T细胞带到肿瘤靶细胞。第二天，我们使用生物发光测定法((参见例如Karimi et.al.,(2014)“通过生物发光成像测量细胞毒性优于标准铬-51释放测定”PLoS ONE 9(2)：e89357)以测定已经裂解的NALM6细胞分数。
细胞毒性测试作为肿瘤再激发进行。由于相对较短的共培养时间和较高的T细胞与肿瘤的比率，标准的单激发离体肿瘤裂解试验通常高估了T细胞的真正抗肿瘤潜力。为了确定存活增强基因(在这种情况下为Bcl-XL)是否也可以增强T细胞功能，我们使用了递归高肿瘤细胞负荷激发以更好地模拟周围的肿瘤微环境来激发T细胞的存活。在总体积为200μl的微滴定板孔中，用1、2、3、4或5个连续剂量的100,000NALM6细胞激发T细胞(100,000)。每100,000个细胞NALM6剂量间隔48小时。对于每次再激发，取出100μl的上清液，并且添加100μl的含有100,000个NALM6细胞的新鲜培养基。在对样品的最后一次激发后24小时，通过加入D荧光素并根据制造商的说明书使用BioTek synergy Neo2混合酶标仪测量发光，以生物发光的减少来测量NALM6细胞死亡。NALM6细胞死亡结果示于表10中。
表10第1行显示了T细胞用NALM6细胞激发的次数。表10第2-5行显示了在不存在任何BiTE的情况下四种不同的T细胞群对NALM6的杀伤作用。在这些条件下的细胞杀伤反映了一般的异源杀伤，没有针对肿瘤抗原的特异性靶向。表达Bcl-XL的3个T细胞群体所实现的杀伤(表10，第2-4行)与初始T细胞(
Figure GDA0003583385470000741
T-cell)所实现的杀伤(表10第5行)非常相当。值得注意的是，与已培养8个月的表达Bcl-XL的T细胞相比，初始T细胞在用于本实验之前仅培养了几周。这表明Bcl-XL表达可以使T细胞在培养物中生长8个月，同时保持它们的细胞毒性。
在存在靶向NALM6细胞表面上的CD19抗原的BiTE的情况下进行第二组激发。表10第9行显示了在存在BiTE的情况下初始T细胞对NALM6的杀伤作用。在第一次和第二次激发后的杀伤比没有BiTE的更有效：在第一次激发时88％的NALM6细胞被杀伤，并且在第二次激发后97％被杀伤。然后杀伤效率降低：在第三次激发后72％的NALM6细胞被杀伤，在第四次激发后62％被杀伤，第五次激发后59％被杀伤。这种降低是长期肿瘤再激发后观察到的T细胞效力丧失的标志(参见例如Voss等人，(2017)Cancer Lett.408：190-196.“代谢重编程和细胞凋亡敏感性：定义T细胞应答的轮廓”)。表达Bcl-XL的3个T细胞群在BiTE存在下进行1或2次激发后在杀伤NALM6方面与初始T细胞一样有效(表10第6-8行)。与天然T细胞不同，表达Bcl-XL的T细胞的NALM6杀伤效率在连续激发后没有降低。在第五次激发后，与初始T细胞59％的杀伤效率相比，表达Bcl-XL的T细胞群体(来自供体81和84)中的两个杀伤95％的NALM6细胞(表10第6行和第8行)，并且第三群体(来自供体82)杀伤94％的NALM6细胞(表10第7行)。这表明，不仅离体生长了8个月的表达Bcl-XL的T细胞仍然能够像仅培养几周的初始T细胞一样杀伤肿瘤细胞；而且在反复暴露于肿瘤抗原后，它们似乎对降低T细胞效力的因素较不敏感。
6.2.1.6b Bcl-XL增强的离体CAR细胞杀伤测试
如6.2.1.3c节所述，我们对表达抗CD19嵌合抗原受体的T细胞进行了肿瘤再激发测试。转座子如6.2.1.3节中所述，包含与生存素或CD28-D124E-T195P共表达的嵌合抗原受体，并且制备了包含与Bcl-XL共表达的嵌合抗原受体的另外的转座子。为了确定存活增强基因(在这种情况下是生存素、CD28-D124E-T195P和Bcl-XL)是否也可以增强T细胞功能，我们使用了递归高肿瘤细胞负荷激发以更好地模拟周围的肿瘤微环境来激发T细胞的存活。在总体积为200μl的微滴定板孔中，用1、2、3、4、5或6个连续剂量的100,000NALM6(其为CD19+、CD20-、CD21-)细胞激发T细胞(100,000)。每100,000个细胞NALM6剂量间隔48小时。对于每次再激发，取出100μl的上清液，并且添加100μl的含有100,000个NALM6细胞的新鲜培养基。在对样品的最后一次激发后24小时，通过添加D荧光素并使用BioTek Synergy Neo2混合酶标仪根据制造商的说明书测量发光，将NALM6细胞死亡测量为生物发光的减少(参见例如Karimi et.al.,(2014)“通过生物发光成像测量细胞毒性优于标准铬-51释放测定”PLoSONE 9(2)：e89357)。
将用转座子转染的细胞离体培养4个月，该转座子包含抗CD19CAR和任选地编码生存素的基因或编码CD28中激活突变的基因或编码Bcl-XL的基因。此时细胞表达了>95％的GFP(并且推断也表达了CAR，如果存在，还表达了存活增强基因)。NALM6细胞死亡结果示于表11中。
表11第1行显示了T细胞用NALM6细胞激发的次数。表11第2行显示了表达抗CD19嵌合抗原受体的T细胞群体对NALM6的杀伤作用。T细胞也表达生存素(第3行)、CD28-D124E-T195P(第4行)或Bcl-XL(第5行)。没有嵌合抗原受体的细胞显示在第6行。仅表达嵌合抗原受体的细胞在第一次激发时杀伤70％的NALM6细胞，在第二次激发时杀伤效率上升至97％，但随后在后续激发时再次下降：第三次激发后69％、第四次激发后59％、第五次激发后58％以及第六次激发后53％的NALM6细胞被杀伤(参见表11第2行)。相比之下，也表达生存素的细胞能够在第六次激发时杀伤85％的NALM6细胞(表11第3行)；也表达CD28-D124E-T195P的细胞能够在第六次激发时杀伤85％的NALM6细胞(表11第4行)，并且也表达Bcl-XL的细胞能够在第六激发时杀伤94％的NALM6细胞(表11第5行)。这表明通过表达嵌合抗原受体的T细胞对生存素或CD28-D124E-T195P或Bcl-XL的表达增强了这些细胞的靶细胞杀伤并降低了细胞变得不能杀伤肿瘤细胞的速率。用于杀伤肿瘤细胞的有利T细胞包含编码嵌合抗原受体的基因和包含可表达的生存素或CD28-D124E-T195P或Bcl-X基因的异源多核苷酸。
本文的凋亡抑制因子生存素、Bcl-XL、Bcl2和Bcl6均显示作为免疫细胞存活基因。预期半胱天冬酶通路中的显性失活基因，例如半胱天冬酶3、半胱天冬酶7、半胱天冬酶8、半胱天冬酶9、半胱天冬酶10或CASP8和FADD样凋亡调节剂(CFLAR)具有类似作用。在本发明的一些实施方案中，免疫细胞包含编码凋亡通路的显性失活抑制因子的基因，该抑制因子包含选自SEQ ID NO：240-245的序列的显性失活突变体；在一些实施方案中，凋亡通路的抑制因子包含选自SEQ ID NO：237、238或261-272的序列。
6.2.2增强型信号传导受体
6.2.2.1抗CD28/OX40是具有增殖增强活性的ESR
设计一种基因以编码抗CD28/OX40 ESR(序列如SEQ ID NO：307所示)，其包含与TNFRSF4(OX40)的跨膜结构域(序列如SEQ ID NO：373所示)和TNFRSF4(OX40)的胞内结构域(序列如SEQ ID NO：341所示)融合的抗CD28抗体TGN1412(序列如SEQ ID NO：340所示)。将该基因与序列如SEQ ID NO：115所示的PGK启动子和如SEQ ID NO：182所示的兔珠蛋白多腺苷酸化信号序列可操作地连接。该基因转移多核苷酸还包含GFP报告基因(序列如SEQ IDNO：222所示)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列如SEQ ID NO：92所示)，另一侧侧翼为D4Z4绝缘子(序列如SEQ ID NO：88所示)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是额外转座子末端序列SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)。基因转移多核苷酸在另一绝缘子的远端还包含额外转座子末端序列SEQ ID NO：5(其与SEQ ID NO：4具有>95％的同一性)，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ IDNO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自2个不同的正常供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书步骤，使用Neon电穿孔仪，用1μg转座子DNA和100ng编码序列如SEQ IDNO：37所示的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。在转染后的1、14和28天取样，用荧光标记的抗CD8抗体孵育，并在荧光激活细胞分选仪(FACS)上分析CD8和Dasher GFP。数据示于表12中。
如6.2.1.1节所述，表达GFP的CD8+细胞的富集是基因转移多核苷酸包含赋予T细胞存活或增殖优势的基因的指示物，如5.3.1.1节所述。用抗CD28/OX40 ESR基因转染的T细胞显示出极快的GFP积累。在来自一个供体的细胞中，94％的CD8+细胞在14天内为GFP+。在来自第二供体的细胞中，98％的细胞在28天内为GFP+。与用对照基因转染的细胞相比，HSV-TK显示相当的初始(第1天)GFP水平，但这些水平降低而不是GFP表达细胞变得富集。该数据表明抗CD28/OX40 ESR的表达为表达ESR的T细胞提供了非常显著的生长/增殖优势。
6.2.2.2 ESR FAS/4-1BB在Casp 7-DN存在下刺激增殖
设计一种基因以编码ESR，其包含TNFRSF6(Fas)的胞外结构域(序列如SEQ ID NO：323所示)，并且还包含TNFRSF6(Fas)的跨膜结构域(序列如SEQ ID NO：387所示)，并且还包含TNFRSF9(4-1BB)的胞内结构域(序列如SEQ ID NO：344所示)。该ESR(Fas/4-1BB)包含序列SEQ ID NO：274。还设计了编码凋亡抑制因子的第二个基因Casp7的显性失活形式：Casp7-DN(序列如SEQ ID NO：262所示)。
将ESR和Casp7-DN分别克隆到基于转座子的基因转移载体中。每个基因与序列如SEQ ID NO：115所示的PGK启动子和序列如SEQ ID NO：182所示的兔珠蛋白多腺苷酸化信号序列可操作地连接。每个基因转移多核苷酸还包含GFP报告基因(序列如SEQ ID NO：222所示)，该GFP报告基因包含可操作地连接至甘油醛-3-磷酸脱氢酶(GAPDH)启动子和牛生长激素(BGH)聚腺苷酸化信号序列的编码DasherGFP的基因。两个开放阅读框被构建为发散转录(两个启动子彼此相邻并且以相反的方向转录)。两个开放阅读框的一侧侧翼为HS4绝缘子(序列如SEQ ID NO：92所示)，另一侧侧翼为D4Z4绝缘子(序列如SEQ ID NO：88所示)。基因转移多核苷酸在一个绝缘子的远端还包含靶序列5'TTAA-3'，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：10(其为SEQ ID NO：6的一个实施方案)，紧接着是额外转座子末端序列SEQ ID NO：3(其与SEQ ID NO：1具有>95％同一性)。基因转移多核苷酸在另一绝缘子的远端还包含额外转座子末端序列SEQ ID NO：5(其与SEQ ID NO：4具有>95％的同一性)，紧接着是piggyBac样转座子反向末端重复序列SEQ ID NO：11(其为SEQ ID NO：7的一个实施方案)，紧接着是靶序列5'TTAA-3'。
使用来自Stemcell Technologies的EasySep Human CD8阳性选择试剂盒，根据制造商的说明书，利用来自2个不同的正常供体的外周血单核细胞(PBMC)制备T细胞。通过与辐射的饲养细胞一起孵育来刺激T细胞2-3天，以提供分泌的CD3、CD28、IL-2、IL-7和IL-15。根据制造商的说明书步骤，使用Neon电穿孔仪，用0.5μg每种转座子DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA转染约100,000个T细胞。将转染的T细胞与饲养细胞混合并在37℃下孵育。在转染后的1、7、28、48和54天取样，用荧光标记的抗CD8抗体孵育，并在荧光激活细胞分选仪(FACS)上分析CD8和Dasher GFP。数据示于表13中。
如6.2.1.1节所述，表达GFP的CD8+细胞的富集是基因转移多核苷酸包含赋予T细胞存活或增殖优势的基因的指示物，如5.3.1.1节所述。用编码ESR FAS/4-1BB和Casp7-DN的基因共转染的T细胞显示表达GFP的细胞的百分比随时间增加。转染后1天，4.5％的CD8+细胞也表达GFP。7天后，1.9％的CD8+细胞表达GFP，表明少于2％的CD8+T细胞已经将基因转移多核苷酸整合到它们的细胞核中。转染后28天，31％的CD8+细胞也表达GFP，表明基因转移多核苷酸上的基因使受体细胞能够更好地存活或更快速地增殖。转染后48天，50.4％的CD8+细胞也表达GFP，且转染后54天，超过97％的CD8+T细胞也表达GFP。该数据表明，ESRFAS/4-1BB和抗凋亡基因Casp7-DN的表达提供了显著的存活/增殖优势。
表格说明
表1.人Jurkat T细胞系中的爪蟾和家蚕piggyBac样转座子
如6.1.1节所述，构建序列如SEQ ID NO：223和224所示的包含piggyBac样转座子的基因转移多核苷酸。根据制造商的说明书，使用Neon电穿孔仪，用1μg质粒DNA和100ng编码相应转座酶mRNA转染Jurkat细胞(每次转染200,000个细胞)。不同时间后(A列中所示)，用抗CD19抗体标记细胞，并通过FACS分析细胞群以测定表达CD19的细胞群的百分比。B列显示了基因转移多核苷酸SEQ ID NO：223中所含的爪蟾转座子的百分比；C列显示了包含在序列如SEQ ID NO：224所示的基因转移多核苷酸内的家蚕转座子的百分比。
表2.Jurkat细胞中异源启动子活性的持续时间
如6.1.2.1节所述，将质粒转染到Jurkat细胞中，该质粒包含A列中命名的启动子(序列如B列中SEQ ID NO所示)和任选的内含子(序列如C列中SEQ ID NO所示)，并且还包含序列如SEQ ID NO：218至启动子的5'所示的多核苷酸和序列如SEQ ID NO：219至启动子的3'所示的多核苷酸。细胞用抗CD19抗体进行荧光标记，并在转染后的不同时间通过流式细胞术进行分析。2天(D列)、8天(E列)、16天(F列)和23天(G列)后显示表面表达CD19的细胞的百分比。H列显示了在第2天和第23天之间表达CD19的细胞的百分比下降。同样的启动子和内含子也与编码GFP的基因可操作地连接，并一式三份瞬时转染到人胚肾(HEK)细胞中。转染后48小时在荧光计上计数细胞。来自3个读数的平均荧光强度显示在I列中。
表3.Jurkat细胞中异源启动子的活性
如6.1.2.1节所述，将质粒转染到Jurkat细胞中，该质粒包含A列中命名的启动子(序列如B列中SEQ ID NO所示)和任选的内含子(序列如C列中SEQ ID NO所示)，并且还包含序列如SEQ ID NO：218至启动子的5'所示的多核苷酸和序列如SEQ ID NO：219至启动子的3'所示的多核苷酸。8天后，用抗CD19抗体对细胞进行荧光标记，并通过流式细胞术进行分析。D列显示平均荧光强度。E列显示计算的Jurkat细胞表面CD19分子的平均数。
表4.原代T细胞中异源启动子活性
如6.1.2.1节所述，将质粒转染到Jurkat细胞中，该质粒包含A列中命名的启动子(序列如B列中SEQ ID NO所示)，还包含序列如SEQ ID NO：218至启动子的5'所示的多核苷酸和序列如SEQ ID NO：219至启动子的3'所示的多核苷酸。11天后，用抗CD19抗体对细胞进行荧光标记，并通过流式细胞术进行分析。C列显示平均荧光强度。
表5.提高原代T细胞的存活率
如6.2.1.2节所述，构建包含piggyBac样转座子的基因转移多核苷酸。每个转座子包含一个推定的存活增强基因。通过共转染1μg单一转座子DNA和100ng编码序列如SEQ IDNO：37(第1-15行)所示的转座酶的mRNA制备15个人原代T细胞样品。基因的名称如A列中所示，基因的SEQ ID NO如B列中所示。通过共转染0.5μg两种不同转座子的DNA(仅在推定的存活增强基因的序列上有所不同)和100ng编码序列如SEQ ID NO：37(第16-23行)所示的转座酶的mRNA制备8个人原代T细胞样品。第一个基因的名称如A列中所示，第一个基因的SEQ IDNO如B列中所示，第二个基因的名称如C列中所示，第二个基因的SEQ ID NO如D列中所示。将细胞培养24天，然后通过FACS分析CD8作为T细胞标志物的存在，以及GFP作为T细胞基因组中基因转移多核苷酸存在指示物的表达。E列显示分析的淋巴细胞百分比，F列显示分析的活细胞百分比，G列显示表面表达CD8的活细胞百分比，H列显示表达GFP的CD8+细胞百分比。
表6.表达生存素和CD28-D124E-T195P的原代T细胞的离体抗肿瘤活性
如6.2.1.3节所述，在piggyBac样转座子上构建编码抗CD19嵌合抗原受体的基因转移多核苷酸。如6.2.1.3节中所述，用转座酶和3个相应转座子其中之一共转染人原代T细胞，该转座子包含编码序列如SEQ ID NO：229所示的嵌合抗原受体的基因和GFP报告基因。一个转座子不包含其他基因(A列)，一个转座子还包含编码生存素的基因(B列)，且一个转座子还包含编码CD28-D124E-T195P的基因(C列)。基因转移多核苷酸的序列以第1行中所示的SEQ ID NO所示。将细胞培养约5周，此时使用FACS测量表达GFP的T细胞的百分比(第2行)。此时，将200,000个T细胞(约200,000个表达GFP的T细胞)与200,000个JY转化的B细胞系混合。混合后3天(第3和4行)或7天(第5和6行)，用荧光标记的抗CD8和抗CD19抗体标记细胞，并使用荧光激活细胞分选仪进行分析。表达CD8的细胞的百分比显示在第4和6行中，表达CD19的细胞的百分比显示在第3和5行中。
表7.表达生存素和CD28-D124E-T195P的原代T细胞的体内抗肿瘤活性
用转座酶和三种相应的基因转移多核苷酸之一共转染人原代T细胞，该基因转移多核苷酸包含如6.2.1.3节和表6所述构建的转座子。转座子的名称显示在A列中，基因转移多核苷酸的序列显示在B列中的SEQ ID NO中。转染后培养细胞约5周，然后用FACS分选以选择表达GFP的细胞，GFP是转座子在T细胞基因组中存在的指示物。将所选择的细胞在培养物中再生长一周，然后通过腹腔注射向先前7天已经接受腹腔注射1百万个JY细胞的小鼠施用1百万个细胞。施用JY细胞后小鼠存活的时间长度(以天计)示于C列中。第1行和第2行没有接受T细胞，而是对照注射磷酸盐缓冲盐水(PBS)。
表8.提高表达生存素和CD28-D124E-T195P的原代T细胞活性
如6.2.1.3节所述，构建包含piggyBac样转座子的基因转移多核苷酸，并将其转染到来自2个不同供体的T细胞中。来自一个供体的细胞离体培养10个月，来自第二个供体的细胞离体培养4个月。如6.2.1.3节所述，通过FACS分选表达GFP的CD8+ T细胞，然后用NALM6B细胞肿瘤系激发。A列显示离体培养时间，B列显示细胞是否表达异源多核苷酸上编码的生存素基因，C列显示细胞是否表达异源多核苷酸上编码的CD28-D124E-T195P基因。D-H列显示使用发光测定观察到的NALM6的杀伤％。D列：在第0天用NALM6激发的细胞并在第1天测量杀伤作用。E列：在第0天和第2天用NALM6激发的细胞，在第3天测量杀伤作用。F列：在第0天、第2天和第4天用NALM6激发的细胞，在第5天测量杀伤作用。G列：在第0天、第2天、第4天和第6天用NALM6激发的细胞，在第7天测量杀伤作用。H列：在第0天、第2天、第4天、第6天和第8天用NALM6激发的细胞，在第9天测量杀伤作用。
表9.提高原代T细胞的存活率
如6.2.1.5节所述，构建包含piggyBac样转座子的基因转移多核苷酸。每个转座子包含一个推定的存活增强基因、ESR基因或对照基因。通过共转染1μg单一转座子的DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA制备来自供体1的人原代T细胞的8个样品(C-F列)和来自供体2的人原代T细胞的8个样品(G-J列)。基因的名称如A列中所示，基因的SEQ ID NO如B列中所示。将细胞培养42天，在转染后的不同时间取样，通过FACS分析CD8作为T细胞标志物的存在，以及GFP作为T细胞基因组中基因转移多核苷酸存在指示物的表达。C-J列显示了在转染后1天(C和G列)、14天(D和H列)、28天(F和J列)和42天(F和J列)，在其表面(即，CD8+ T细胞)表达CD8且也表达GFP的分析细胞的百分比。
表10.提高表达Bcl-XL的原代T细胞的活性
如6.2.1.6节所述，构建包含piggyBac样转座子的基因转移多核苷酸，并将其转染到来自3个不同供体的T细胞中。如6.2.1.6节所述，240天后，用NALM6 B细胞肿瘤系激发细胞。A列显示供体ID，B列显示细胞是否含有包含编码Bcl-XL的基因的转座子，C列显示培养物是否还含有CD3/CD19结合BiTE。D-H列显示使用发光测定观察到的NALM6的杀伤％。D列：在第0天用NALM6激发的细胞并在第1天测量杀伤作用。E列：在第0天和第2天用NALM6激发的细胞，在第3天测量杀伤作用。F列：在第0天、第2天和第4天用NALM6激发的细胞，在第5天测量杀伤作用。G列：在第0天、第2天、第4天和第6天用NALM6激发的细胞，在第7天测量杀伤作用。H列：在第0天、第2天、第4天、第6天和第8天用NALM6激发的细胞，在第9天测量杀伤作用。
表11.提高表达生存素和CD28-D124E-T195P的原代T细胞活性
如6.2.1.6b节所述，构建包含piggyBac样转座子的基因转移多核苷酸，并将其转染到T细胞中。如6.2.1.6b节所述，通过FACS分选表达GFP的CD8+ T细胞，然后用NALM6 B细胞肿瘤系激发。A列显示细胞是否表达异源多核苷酸上编码的生存素基因，B列显示细胞是否表达异源多核苷酸上编码的CD28-D124E-T195P基因，C列显示细胞是否表达异源多核苷酸上编码的Bcl-XL基因。D-I列显示使用发光测定观察到的NALM6的杀伤％。D列：在第0天用NALM6激发的细胞并在第1天测量杀伤作用。E列：在第0天和第2天用NALM6激发的细胞，在第3天测量杀伤作用。F列：在第0天、第2天和第4天用NALM6激发的细胞，在第5天测量杀伤作用。G列：在第0天、第2天、第4天和第6天用NALM6激发的细胞，在第7天测量杀伤作用。H列：在第0天、第2天、第4天、第6天和第8天用NALM6激发的细胞，在第9天测量杀伤作用。I列：在第0天、第2天、第4天、第6天、第8天和第10天用NALM6激发的细胞，在第11天测量杀伤作用。
表12.抗CD28/OX40 ESR刺激原代T淋巴细胞增殖的研究
如6.2.2.1节所述，构建包含在piggyBac样转座子上编码的抗CD28/OX40 ESR的基因转移多核苷酸。对照转座子包含单纯疱疹病毒胸苷激酶(HSV-TK)基因而非ESR。通过共转染1μg转座子的DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA制备来自两个供体的人原代T细胞样品。将细胞培养如A列中所示的天数，然后通过FACS分析CD8作为T细胞标志物的存在，以及GFP作为T细胞基因组中基因转移多核苷酸存在的指示物的表达。也表达GFP的表达CD8的细胞的百分比示于B-E列中：用抗CD28/OX40 ESR转染的供体1细胞(B列)，用HSV-TK转染的供体1细胞(B列)，用抗CD28/OX40 ESR转染的供体2细胞(C列)，用HSV-TK转染的供体2细胞(D列)。ND：未测试。
表13.ESR FAS/4-1BB和Casp7-DN刺激原代T细胞增殖
如6.2.2.2节所述，在piggyBac样转座子上构建编码ESR的基因转移多核苷酸，其中FAS的胞外结构域与4-1BB的跨膜和胞内结构域融合。如6.2.2.2节所述，在第二piggyBac样转座子上构建编码凋亡Casp7-DN的显性失活抑制因子的第二基因转移多核苷酸。通过共转染0.5μg每种转座子的DNA和100ng编码序列如SEQ ID NO：37所示的转座酶的mRNA制备来自两个供体的人原代T细胞样品。将细胞培养如A列中所示的天数，然后通过FACS分析CD8作为T细胞标志物的存在，以及GFP作为T细胞基因组中基因转移多核苷酸存在的指示物的表达。B列显示也表达GFP的表达CD8细胞的百分比。
表格
Figure GDA0003583385470000851
表1
Figure GDA0003583385470000852
表2
Figure GDA0003583385470000853
表3
Figure GDA0003583385470000861
表4
Figure GDA0003583385470000871
表5
Figure GDA0003583385470000872
表6
Figure GDA0003583385470000881
表7
Figure GDA0003583385470000882
表8
Figure GDA0003583385470000883
表9
Figure GDA0003583385470000891
表10
Figure GDA0003583385470000892
表11
Figure GDA0003583385470000893
表12
Figure GDA0003583385470000901
表13
本发明包括下列实施例：
1.一种包含免疫细胞存活增强基因的多核苷酸，所述免疫细胞存活增强基因包含与异源调控序列可操作地连接的编码蛋白质的核酸，所述异源调控序列对所述蛋白质在免疫细胞内的表达有效，从而增强所述免疫细胞的存活。
2.根据实施例1所述的多核苷酸，其中所述免疫细胞存活增强基因编码包含激活突变的天然存在的蛋白质。
3.根据实施例1或2所述的多核苷酸，其中所述免疫细胞存活增强基因编码选自包含激活突变的STAT3、CD28、RhoA、PLCG、STAT5B或CCND1的蛋白质。
4.根据实施例3所述的多核苷酸，其中所述免疫细胞存活增强基因编码STAT3，其中所述STAT3包含以下激活突变中的一个或多个：F174S、H410R、S614R、E616K、G618R、Y640F、N647I、E652K、K658Y、K658R、K658N、K658M、K658R、K658H、K658N、D661Y或D661V。
5.根据实施例3所述的多核苷酸，其中所述免疫细胞存活增强基因编码CD28，其中所述CD28包含以下激活突变中的一个或多个：D124E、D124V、T195I或T195P。
6.根据实施例3所述的多核苷酸，其中所述免疫细胞存活增强基因编码RhoA，其中所述RhoA包含以下激活突变中的一个或多个：G17V或K18N。
7.根据实施例3所述的多核苷酸，其中所述免疫细胞存活增强基因编码PLCG，其中所述PLCG包含以下激活突变中的一个或多个：S345F、S520F或R707Q。
8.根据实施例3所述的多核苷酸，其中所述免疫细胞存活增强基因编码STAT5B，其中所述STAT5B包含以下激活突变中的一个或多个：N642H、T648S、S652Y、Y665F或P267A。
9.根据实施例3所述的多核苷酸，其中所述免疫细胞存活增强基因编码CCND1，其中所述CCND1包含以下激活突变中的一个或多个：E36G、E36Q、E36K、A39S、S41L、S41P、S41T、V42E、V42A、V42L、V42M、Y44S、Y44D、Y44C、Y44H、K46T、K46R、K46N、K46E、C47G、C47R、C47S、C47W、P199R、P199S、P199L、S201F、T285I、T285A、P286L、P286H、P286S、P286T或P286A。
10.根据实施例1所述的多核苷酸，其中所述免疫细胞存活增强基因编码天然存在的人蛋白质。
11.根据实施例10所述的多核苷酸，其中所述免疫细胞存活增强基因编码选自生存素(Survivin)、Bcl2、Bcl6或Bcl-XL的蛋白质。
12.根据实施例1所述的多核苷酸，其中所述免疫细胞存活增强基因编码凋亡抑制因子。
13.根据任一前述实施例所述的多核苷酸，其中所述异源启动子选自EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子。
14.根据实施例1-12所述的多核苷酸物，其中所述异源启动子选自SEQ ID No：94-154。
15.根据任一前述实施例所述的多核苷酸，其中所述多核苷酸还包含选自SEQ IDNO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21、或SEQ ID NO：26和27、或SEQ ID NO：399和400的序列对。
16.根据任一前述实施例所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的半衰期，所述基因组包含所述多核苷酸的免疫细胞的半衰期增加至少25％。
17.根据任一前述实施例所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的最大寿命，所述基因组包含所述多核苷酸的免疫细胞的最大寿命增加至少25％。
18.根据任一前述实施例所述的多核苷酸，其中相对于基因组包含所述多核苷酸的免疫细胞的倍增时间，所述基因组不包含所述多核苷酸的免疫细胞的倍增时间高出至少25％。
19.根据任一前述实施例所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的增殖速率，所述基因组包含所述多核苷酸的免疫细胞的增殖速率增加至少25％。
20.根据任一前述实施例所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的存活率，所述基因组包含所述多核苷酸的T细胞在重复抗原激发后的存活率增加至少25％。
21.一种转座子，其包含前述实施例中任一项所述的多核苷酸。
22.一种慢病毒载体，其包含实施例1-20中任一项所述的多核苷酸。
23.一种产生修饰的免疫细胞的方法，所述方法包括将与异源启动子可操作地连接的编码凋亡抑制因子的多核苷酸导入免疫细胞。
24.根据实施例23所述的方法，其中所述多核苷酸还包含转座子末端，并且其中所述方法还包括将相应的转座酶导入所述免疫细胞，使得编码所述凋亡抑制因子的多核苷酸被转座到所述免疫细胞的基因组中。
25.根据实施例23或24所述的方法，其中所述转座酶作为编码转座酶的核酸被导入。
26.根据实施例25所述的方法，其中所述核酸为mRNA。
27.根据实施例24或25所述的方法，其中编码所述转座酶的所述核酸与在所述免疫细胞中有活性的启动子可操作地连接。
28.根据实施例24所述的方法，其中将所述转座子和转座酶同时导入所述免疫细胞中。
29.根据实施例24所述的方法，其中将所述转座子和转座酶在不同时间导入所述免疫细胞中。
30.根据实施例23-29中任一项所述的方法，其中所述免疫细胞为T细胞，所述方法还包括将编码能够结合抗原的受体的基因导入所述免疫细胞，其中所述受体与在其表面展示所述抗原的靶细胞结合导致所述T细胞杀伤所述靶细胞。
31.根据实施例23-29中任一项所述的方法，其中所述凋亡抑制因子选自生存素、Bcl2、Bcl6、Bcl-XL或Casp3、Casp7、Casp8、Casp9或Casp10的显性失活突变体。
32.一种产生修饰的免疫细胞的方法，所述方法包括将编码选自STAT3、CD28、RhoA、PLCG、STAT5B或CCND1的蛋白质的多核苷酸导入免疫细胞，其中所述蛋白质包含与异源启动子可操作地连接的激活突变。
33.一种产生修饰的免疫细胞的方法，所述方法包括将编码多肽的多核苷酸导入免疫细胞，所述多肽包含
a.衍生自通常向免疫细胞传递抑制信号的受体的胞外结构域的序列
b.衍生自向免疫细胞传递刺激信号的受体的胞内结构域的序列
c.跨膜结构域
并且其中所述多肽不包含CD3ζ胞内结构域。
34.根据实施例33所述的方法，其中所述胞外结构域包含选自SEQ ID NO：322-340的序列。
35.根据实施例33所述的方法，其中所述胞内结构域包含选自SEQ ID NO：341-364的序列。
36.根据实施例33或34所述的方法，其中所述多肽包含选自SEQ ID NO：274-318的序列。
37.一种免疫细胞，其基因组包含实施例1-22中任一项所述的多核苷酸。
38.根据实施例37所述的免疫细胞，其中相对于基因组不包含实施例1所述的多核苷酸的免疫细胞的半衰期，所述免疫细胞的半衰期增加至少25％。
39.根据实施例37或38所述的免疫细胞，其中相对于基因组不包含实施例1所述的多核苷酸的免疫细胞的最大寿命，所述免疫细胞的最大寿命增加至少25％。
40.根据实施例37-39中任一项所述的免疫细胞，其中相对于基因组包含实施例1所述的多核苷酸的免疫细胞的半衰期，所述基因组不包含根据实施例1所述的多核苷酸的免疫细胞的倍增时间增加至少25％。
41.根据实施例37-40中任一项所述的免疫细胞，其中相对于基因组不包含实施例1所述的多核苷酸的免疫细胞的增殖速率，所述免疫细胞的增殖速率增加至少25％。
42.根据实施例37-41中任一项所述的免疫细胞，其中相对于基因组不包含所述多核苷酸的免疫细胞的存活率，所述基因组包含所述多核苷酸的T细胞在重复抗原激发后的存活率增加至少25％。
43.根据实施例37-42中任一项所述的免疫细胞，其中所述免疫细胞为T细胞。
44.根据实施例37-42中任一项所述的免疫细胞，其中所述免疫细胞为B细胞。
45.根据实施例37-42中任一项所述的免疫细胞，其中所述免疫细胞为人细胞。
46.根据实施例37-42中任一项所述的免疫细胞，其中所述免疫细胞为灵长类细胞、啮齿动物细胞、猫细胞、狗细胞或马细胞。
47.根据实施例1所述的多核苷酸，其中所述免疫细胞存活增强基因编码增强型信号传导受体(ESR)，其中所述ESR包含：
a.衍生自通常向免疫细胞传递抑制信号的受体的胞外结构域的序列
b.衍生自向免疫细胞传递刺激信号的受体的胞内结构域的序列
c.跨膜结构域
并且其中所述ESR不包含CD3ζ胞内结构域
48.根据实施例47所述的多核苷酸，其中所述胞外结构域(a)衍生自选自以下的人蛋白质：TNFRSF3(LTRβ)、TNFRSF6(Fas)、TNFRSF8(CD30)、TNFRSF10A(DR4)、TNFRSF10B(DR5)、TNFRSF19(TROY)、TNFRSF21(DR6)和CTLA4。
49.根据实施例47所述的多核苷酸，其中所述ESR包含与选自SEQ ID NO：322-340的序列具有至少90％同一性的序列。
50.根据实施例47-49中任一项所述的多核苷酸，其中所述胞内结构域(b)衍生自选自以下的人蛋白质：TNFRSF4(OX40)、TNFRSF5(CD40)、TNFRSF7(CD27)、TNFRSF9(4-1BB)、TNFRSF11A(RANK)、TNFRSF13B(TACI)、TNFRSF13C(BAFF-R)、TNFRSF14(HVEM)、TNFRSF17(CD269)、TNFRSF18(GITR)、CD28、CD28H(TMIGD2)、诱导型T细胞共刺激因子(ICOS/CD278)、DNAX辅助分子1(DNAM-1/CD226)、信号转导淋巴细胞激活分子(SLAM/CD150)、T细胞免疫球蛋白和粘蛋白结构域(TIM-1/HAVcr-1)、干扰素受体α链(IFNAR1)、干扰素受体β链(IFNAR2)、白细胞介素2受体β亚基(IL2RB)、白细胞介素2受体γ亚基(IL2RG)、肿瘤坏死因子超家族14(TNFSF14/LIGHT)、自然杀伤细胞2族成员D(NKG2D/CD314)和CD40配体(CD40L)。
51.根据实施例47-50中任一项所述的多核苷酸，其中所述ESR包含与选自SEQ IDNO：341-364的序列具有至少90％同一性的序列。
52.根据实施例47-51中任一项所述的多核苷酸，其中所述ESR包含与选自SEQ IDNO：365-396的序列具有至少90％同一性的序列。
53.根据实施例45-52中任一项所述的多核苷酸，其中所述ESR包含与选自SEQ IDNO：274-318的序列具有至少90％同一性的序列。
54.根据实施例47-53中任一项所述的多核苷酸，其中所述多核苷酸还包含与异源启动子可操作地连接的编码凋亡抑制因子的片段。
55.一种免疫细胞，其基因组包含实施例47-54中任一项所述的多核苷酸。
56.根据实施例55所述的免疫细胞，其中所述免疫细胞基因组还包含与异源启动子可操作地连接的编码凋亡抑制因子的片段。
57.一种产生修饰的免疫细胞的方法，所述方法包括
a.向所述免疫细胞中导入实施例47所述的多核苷酸。
b.将与异源启动子可操作地连接的编码凋亡抑制因子的多核苷酸导入所述免疫细胞中。
58.根据实施例57所述的方法，其中将这两种多核苷酸同时导入所述免疫细胞中。
59.一种鉴定提高免疫细胞存活率的蛋白质的方法，包括
对来自癌性免疫细胞的编码蛋白质的核酸进行测序以鉴定编码具有突变的蛋白质的核酸；
用编码具有突变的所述蛋白质的核酸转化免疫细胞；以及确定所述免疫细胞是否具有提高的存活率。
7.参考文献
本文引用的所有参考文献通过引用整体并入本文，并且出于所有目的，并入程度如同每个单独的出版物或专利或专利申请被具体地和单独地指示为通过引用整体并入本文。如果不同的内容与在不同时间的引用相关联，则意味着与在本发明的优先权日的引用相关联的内容。
在不脱离本发明的精神和范围的情况下，可以对本发明进行许多修改和改变，这对于本领域的技术人员来说是显而易见的。本文所述的具体实施方案仅通过举例的方式提供，并且本发明仅受所附权利要求的术语以及这些权利要求所赋予的等同物的全部范围的限制。
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<223> 未知或合成序列
<400> 4
cctgggtaaa ctaaaagtcc cctcgaggaa aggcccctaa agtgaaacag tgcaaaacgt 60
tcaaaaactg tctggcaata caagttccac tttgggacaa atcggc 106
<210> 5
<211> 105
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 5
cctgggtaaa ctaaaagtcc cctcgaggaa aggcccctaa agtgaaacag tgcaaaacgt 60
tcaaaaactg tctggcaata caagttccac tttgaccaaa acggc 105
<210> 6
<211> 14
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 6
ccytttbmct gcca 14
<210> 7
<211> 14
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 7
tggcagkvaa argg 14
<210> 8
<211> 14
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 8
ccctttgcct gcca 14
<210> 9
<211> 14
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 9
tggcagtgaa aggg 14
<210> 10
<211> 14
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 10
cctttttact gcca 14
<210> 11
<211> 14
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 11
tggcagtaaa aggg 14
<210> 12
<211> 205
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 12
cagggtatct cataccctgg taaaatttta aagttgtgta ttttataaaa ttttcgtctg 60
acaacactag cgcgctcagt agctggaggc aggagcgtgc gggaggggat agtggcgtga 120
tcgcagtgtg gcacgggaca ccggcgagat attcgtgtgc aaacctgttt cgggtatgtt 180
ataccctgcc tcattgttga cgtat 205
<210> 13
<211> 192
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 13
tttaagaaaa agattaataa ataataataa tttcataatt aaaaacttct ttcattgaat 60
gccattaaat aaaccattat tttacaaaat aagatcaaca taattgagta aataataata 120
agaacaatat tatagtacaa caaaatatgg gtatgtcata ccctgccaca ttcttgatgt 180
aacttttttt ca 192
<210> 14
<211> 16
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 14
cccggcgagc atgagg 16
<210> 15
<211> 16
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 15
cctcatgctc gccggg 16
<210> 16
<211> 309
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 16
ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt 60
tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc 120
ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt 180
gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa 240
ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt 300
atagatatc 309
<210> 17
<211> 231
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 17
tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat 60
aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat 120
atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt 180
ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg g 231
<210> 18
<211> 13
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 18
ccctagaaag ata 13
<210> 19
<211> 13
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 19
tatctttcta ggg 13
<210> 20
<211> 15
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 20
cacttggatt gcggg 15
<210> 21
<211> 15
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 21
cccgacaccg tagtg 15
<210> 22
<211> 262
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 22
aaacgagtta agtcggctcg cgtgaattgc gcgtactccg cgggagccgt cttaactcgg 60
ttcatataga tttgcggtgg agtgcgggaa acgtgtaaac tcgggccgat tgtaactgcg 120
tattaccaaa tatttgtttc caagcttggt accgagctcg gatcccgtac gctgcaggtc 180
gacggatccc cgggttaatt aaggcgcgcc agatctgttt agcttgcctc gtccccgccg 240
ggtcacccgg ccagcgacat gg 262
<210> 23
<211> 227
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 23
tgtcgaagaa ttcggcggcc gcatgcatct agagaattat ttatgtactg aatagataaa 60
aaaatgtctg tgattgaata aattttcatt ttttacacaa gaaaccgaaa atttcatttc 120
aatcgaaccc atacttcaaa agatataggc attttaaact aactctgatt ttgcgcggga 180
aacctaaata attgcccgcg ccatcttata ttttggcggg aaattca 227
<210> 24
<211> 227
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 24
cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac 60
tacaccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact 120
ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata 180
attcactgta tcacaattcc agtgggtcag aagtttacat acactaa 227
<210> 25
<211> 229
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 25
ttgagtgtat gttaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat 60
gaatcattct ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct 120
aactgacctt aagacaggga atctttactc ggattaaatg tcaggaattg tgaaaaagtg 180
agtttaaatg tatttggcta aggtgtatgt aaacttccga cttcaactg 229
<210> 26
<211> 32
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 26
cagttgaagt cggaagttta catacactta ag 32
<210> 27
<211> 32
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 27
ctaaggtgta tgtaaacttc cgacttcaac tg 32
<210> 28
<211> 340
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 28
Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val
1 5 10 15
Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu
20 25 30
Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His
35 40 45
His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu
50 55 60
Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro
65 70 75 80
Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr
85 90 95
Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu
100 105 110
Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys
115 120 125
Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr
130 135 140
Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe
145 150 155 160
Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys
165 170 175
Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile
180 185 190
Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys
195 200 205
Ile Asp Gly Ile Met Asp Lys Glu Asn Tyr Val Asp Ile Leu Lys Gln
210 215 220
His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val
225 230 235 240
Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys
245 250 255
Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser
260 265 270
Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg
275 280 285
Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys
290 295 300
Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val
305 310 315 320
Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn
325 330 335
Ala Thr Lys Tyr
340
<210> 29
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 29
Met Ala Gln His Ser Asp Tyr Ser Asp Asp Glu Phe Cys Ala Asp Lys
1 5 10 15
Leu Ser Asn Tyr Ser Cys Asp Ser Asp Leu Glu Asn Ala Ser Thr Ser
20 25 30
Asp Glu Asp Ser Ser Asp Asp Glu Val Met Val Arg Pro Arg Thr Leu
35 40 45
Arg Arg Arg Arg Ile Ser Ser Ser Ser Ser Asp Ser Glu Ser Asp Ile
50 55 60
Glu Gly Gly Arg Glu Glu Trp Ser His Val Asp Asn Pro Pro Val Leu
65 70 75 80
Glu Asp Phe Leu Gly His Gln Gly Leu Asn Thr Asp Ala Val Ile Asn
85 90 95
Asn Ile Glu Asp Ala Val Lys Leu Phe Ile Gly Asp Asp Phe Phe Glu
100 105 110
Phe Leu Val Glu Glu Ser Asn Arg Tyr Tyr Asn Gln Asn Arg Asn Asn
115 120 125
Phe Lys Leu Ser Lys Lys Ser Leu Lys Trp Lys Asp Ile Thr Pro Gln
130 135 140
Glu Met Lys Lys Phe Leu Gly Leu Ile Val Leu Met Gly Gln Val Arg
145 150 155 160
Lys Asp Arg Arg Asp Asp Tyr Trp Thr Thr Glu Pro Trp Thr Glu Thr
165 170 175
Pro Tyr Phe Gly Lys Thr Met Thr Arg Asp Arg Phe Arg Gln Ile Trp
180 185 190
Lys Ala Trp His Phe Asn Asn Asn Ala Asp Ile Val Asn Glu Ser Asp
195 200 205
Arg Leu Cys Lys Val Arg Pro Val Leu Asp Tyr Phe Val Pro Lys Phe
210 215 220
Ile Asn Ile Tyr Lys Pro His Gln Gln Leu Ser Leu Asp Glu Gly Ile
225 230 235 240
Val Pro Trp Arg Gly Arg Leu Phe Phe Arg Val Tyr Asn Ala Gly Lys
245 250 255
Ile Val Lys Tyr Gly Ile Leu Val Arg Leu Leu Cys Glu Ser Asp Thr
260 265 270
Gly Tyr Ile Cys Asn Met Glu Ile Tyr Cys Gly Glu Gly Lys Arg Leu
275 280 285
Leu Glu Thr Ile Gln Thr Val Val Ser Pro Tyr Thr Asp Ser Trp Tyr
290 295 300
His Ile Tyr Met Asp Asn Tyr Tyr Asn Ser Val Ala Asn Cys Glu Ala
305 310 315 320
Leu Met Lys Asn Lys Phe Arg Ile Cys Gly Thr Ile Arg Lys Asn Arg
325 330 335
Gly Ile Pro Lys Asp Phe Gln Thr Ile Ser Leu Lys Lys Gly Glu Thr
340 345 350
Lys Phe Ile Arg Lys Asn Asp Ile Leu Leu Gln Val Trp Gln Ser Lys
355 360 365
Lys Pro Val Tyr Leu Ile Ser Ser Ile His Ser Ala Glu Met Glu Glu
370 375 380
Ser Gln Asn Ile Asp Arg Thr Ser Lys Lys Lys Ile Val Lys Pro Asn
385 390 395 400
Ala Leu Ile Asp Tyr Asn Lys His Met Lys Gly Val Asp Arg Ala Asp
405 410 415
Gln Tyr Leu Ser Tyr Tyr Ser Ile Leu Arg Arg Thr Val Lys Trp Thr
420 425 430
Lys Arg Leu Ala Met Tyr Met Ile Asn Cys Ala Leu Phe Asn Ser Tyr
435 440 445
Ala Val Tyr Lys Ser Val Arg Gln Arg Lys Met Gly Phe Lys Met Phe
450 455 460
Leu Lys Gln Thr Ala Ile His Trp Leu Thr Asp Asp Ile Pro Glu Asp
465 470 475 480
Met Asp Ile Val Pro Asp Leu Gln Pro Val Pro Ser Thr Ser Gly Met
485 490 495
Arg Ala Lys Pro Pro Thr Ser Asp Pro Pro Cys Arg Leu Ser Met Asp
500 505 510
Met Arg Lys His Thr Leu Gln Ala Ile Val Gly Ser Gly Lys Lys Lys
515 520 525
Asn Ile Leu Arg Arg Cys Arg Val Cys Ser Val His Lys Leu Arg Ser
530 535 540
Glu Thr Arg Tyr Met Cys Lys Phe Cys Asn Ile Pro Leu His Lys Gly
545 550 555 560
Ala Cys Phe Glu Lys Tyr His Thr Leu Lys Asn Tyr Leu Glu Lys Arg
565 570 575
Gly Pro Ser Leu Arg Leu Ala Val Val Leu Gln His Arg Lys Ser Leu
580 585 590
<210> 30
<211> 594
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 30
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln
1 5 10 15
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp
20 25 30
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile
35 40 45
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu
50 55 60
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn
65 70 75 80
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His
85 90 95
Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu
100 105 110
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile
115 120 125
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile
130 135 140
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg
145 150 155 160
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile
165 170 175
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn
180 185 190
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr
195 200 205
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu
210 215 220
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val
225 230 235 240
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile
245 250 255
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu
260 265 270
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro
275 280 285
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys
290 295 300
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn
305 310 315 320
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val
325 330 335
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile
340 345 350
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val
355 360 365
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn
370 375 380
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro
385 390 395 400
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu
405 410 415
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys
420 425 430
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr
435 440 445
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg
450 455 460
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn
465 470 475 480
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val
485 490 495
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser
500 505 510
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu
515 520 525
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser
530 535 540
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr
545 550 555 560
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys
565 570 575
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser
580 585 590
Cys Phe
<210> 31
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 31
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 32
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 32
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Pro Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asn Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Asp His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Arg Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Thr Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Ser Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Leu Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 33
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 33
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Tyr
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 34
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 34
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Val Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Asn Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu Glu Phe Asn Asn Glu Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 35
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 35
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Gln Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Lys
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Val Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Asp Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 36
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 36
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Cys Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Val Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Asp Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 37
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 37
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Gln Thr Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Ile Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Lys Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 38
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 38
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Gly Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 39
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 39
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala His Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Gly Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Ile Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu His Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Ser Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 40
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 40
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Gly Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Ile Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Asp Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 41
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 41
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Gln Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Gly Ala Arg Ala His Ala Trp Tyr Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Ile Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Gly Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 42
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 42
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Gly Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Ile Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Met Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Lys Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Ser Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 43
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 43
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Tyr Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Lys
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Asp Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Ile Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 44
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 44
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Cys Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Ile Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Val Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 45
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 45
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp Tyr Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 46
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 46
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Ser
1 5 10 15
Ala Ser Ser Ser Asp Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Gly Ala Arg Ala His Ala Trp Tyr Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Met Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Thr Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Lys Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Ser Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 47
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 47
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Gly Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Asn Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 48
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 48
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Thr Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg His Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Ala Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 49
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 49
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Glu Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 50
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 50
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 51
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 51
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Arg
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 52
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 52
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Lys Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asn Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg His Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr
580 585
<210> 53
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 53
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Gln Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Arg Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Tyr His Tyr
580 585
<210> 54
<211> 589
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 54
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Pro Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr His Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Tyr His Tyr
580 585
<210> 55
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 55
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Gln Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Val Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Lys Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 56
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 56
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Lys Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Gly Thr Val His
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 57
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 57
Met Ala Lys Arg Phe Cys Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Ala Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 58
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 58
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Val Pro Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Lys Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 59
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 59
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Gln Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Lys Leu Thr Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 60
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 60
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Val Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Asp Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Thr Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 61
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 61
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Pro
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Val Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu His Phe Asn Asn Glu Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 62
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 62
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Val Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Asn Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Ser Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Lys Phe Leu His Phe Asn Asn Glu Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 63
<211> 592
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 63
Met Ala Lys Arg Phe Tyr Ser Ala Glu Glu Ala Ala Ala His Cys Met
1 5 10 15
Ala Ser Ser Ser Glu Glu Phe Ser Gly Ser Asp Ser Glu Tyr Val Pro
20 25 30
Pro Ala Ser Glu Ser Asp Ser Ser Thr Glu Glu Ser Trp Cys Ser Ser
35 40 45
Ser Thr Val Ser Ala Leu Glu Glu Pro Met Glu Val Asp Glu Asp Val
50 55 60
Asp Asp Leu Glu Asp Gln Glu Ala Gly Asp Arg Ala Asp Ala Ala Ala
65 70 75 80
Gly Gly Glu Pro Ala Trp Gly Pro Pro Cys Asn Phe Pro Pro Glu Ile
85 90 95
Pro Pro Phe Thr Thr Val Pro Gly Val Lys Val Asp Thr Ser Asn Phe
100 105 110
Glu Pro Ile Asn Phe Phe Gln Leu Phe Met Thr Glu Ala Ile Leu Gln
115 120 125
Asp Met Val Leu Tyr Thr Asn Val Tyr Ala Glu Gln Tyr Leu Thr Gln
130 135 140
Val Pro Leu Pro Arg Tyr Ala Arg Ala His Ala Trp His Pro Thr Asp
145 150 155 160
Ile Ala Glu Met Lys Arg Phe Val Gly Leu Thr Leu Ala Met Gly Leu
165 170 175
Ile Lys Ala Asn Ser Leu Glu Ser Tyr Trp Asp Thr Thr Thr Val Leu
180 185 190
Asn Ile Pro Val Phe Ser Ala Thr Met Ser Arg Asn Arg Tyr Gln Leu
195 200 205
Leu Leu Arg Phe Leu Glu Phe Asn Asn Asn Ala Thr Ala Val Pro Pro
210 215 220
Asp Gln Pro Gly His Asp Arg Leu His Lys Leu Arg Pro Leu Ile Asp
225 230 235 240
Ser Leu Ser Glu Arg Phe Ala Ala Val Tyr Thr Pro Cys Gln Asn Ile
245 250 255
Cys Ile Asp Glu Ser Leu Leu Leu Phe Lys Gly Arg Leu Gln Phe Arg
260 265 270
Gln Tyr Ile Pro Ser Lys Arg Ala Arg Tyr Gly Ile Lys Phe Tyr Lys
275 280 285
Leu Cys Glu Ser Ser Ser Gly Tyr Thr Ser Tyr Phe Leu Ile Tyr Glu
290 295 300
Gly Lys Asp Ser Lys Leu Asp Pro Pro Gly Cys Pro Pro Asp Leu Thr
305 310 315 320
Val Ser Gly Lys Ile Val Trp Glu Leu Ile Ser Pro Leu Leu Gly Gln
325 330 335
Gly Phe His Leu Tyr Val Asp Asn Phe Tyr Ser Ser Ile Pro Leu Phe
340 345 350
Thr Ala Leu Tyr Cys Leu Asp Thr Pro Ala Cys Gly Thr Ile Asn Arg
355 360 365
Asn Arg Lys Gly Leu Pro Arg Ala Leu Leu Asp Lys Lys Leu Asn Arg
370 375 380
Gly Glu Thr Tyr Ala Leu Arg Lys Asn Glu Leu Leu Ala Ile Lys Phe
385 390 395 400
Phe Asp Lys Lys Asn Val Phe Met Leu Thr Ser Ile His Asp Glu Ser
405 410 415
Val Ile Arg Glu Gln Arg Val Gly Arg Pro Pro Lys Asn Lys Pro Leu
420 425 430
Cys Ser Lys Glu Tyr Ser Lys Tyr Met Gly Gly Val Asp Arg Thr Asp
435 440 445
Gln Leu Gln His Tyr Tyr Asn Ala Thr Arg Lys Thr Arg Ala Trp Tyr
450 455 460
Lys Lys Val Gly Ile Tyr Leu Ile Gln Met Ala Leu Arg Asn Ser Tyr
465 470 475 480
Ile Val Tyr Lys Ala Ala Val Pro Gly Pro Lys Leu Ser Tyr Tyr Lys
485 490 495
Tyr Gln Leu Gln Ile Leu Pro Ala Leu Leu Phe Gly Gly Val Glu Glu
500 505 510
Gln Thr Val Pro Glu Met Pro Pro Ser Asp Asn Val Ala Arg Leu Ile
515 520 525
Gly Lys His Phe Ile Asp Thr Leu Pro Pro Thr Pro Gly Lys Gln Arg
530 535 540
Pro Gln Lys Gly Cys Lys Val Cys Arg Lys Arg Gly Ile Arg Arg Asp
545 550 555 560
Thr Arg Tyr Tyr Cys Pro Lys Cys Pro Arg Asn Pro Gly Leu Cys Phe
565 570 575
Lys Pro Cys Phe Glu Ile Tyr His Thr Gln Leu His Tyr Gly Arg Arg
580 585 590
<210> 64
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 64
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 65
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 65
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Leu Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val His Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Val Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala His Leu Asp Ser
595 600 605
Ser Leu
610
<210> 66
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 66
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Glu Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Gln Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Lys
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Ile
610
<210> 67
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 67
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Ala Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Leu Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Cys Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 68
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 68
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Met Ser Gly Pro
85 90 95
His Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Ser Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
His Leu
610
<210> 69
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 69
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Ser Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Leu Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Ala Met Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 70
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 70
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Leu Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Thr Gln Ile Pro Glu Asn Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Gln Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 71
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 71
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Gln Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Lys
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Tyr Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 72
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 72
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 73
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 73
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Trp Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Lys
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Tyr Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu His Ser
595 600 605
Ser Leu
610
<210> 74
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 74
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Ser Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 75
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 75
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 76
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 76
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Tyr Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Gln Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Tyr Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 77
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 77
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Lys Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Leu Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Ile Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val His Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Lys Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 78
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 78
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Ile Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Ile Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Trp Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 79
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 79
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Ala Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Tyr Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 80
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 80
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Pro Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Val
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Val Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 81
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 81
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg Glu Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Lys Asn Leu Glu Val Tyr Val Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 82
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 82
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Ile Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Asp Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Tyr Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 83
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 83
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Tyr Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gly Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 84
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 84
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Ile Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Val Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 85
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 85
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Gly Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Ser Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Leu Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Lys Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Tyr Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Asp Ser
595 600 605
Ser Leu
610
<210> 86
<211> 610
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 86
Met Asp Ile Glu Arg Gln Glu Glu Arg Ile Arg Ala Met Leu Glu Glu
1 5 10 15
Glu Leu Ser Asp Tyr Ser Asp Glu Ser Ser Ser Glu Asp Glu Thr Asp
20 25 30
His Cys Ser Glu His Glu Val Asn Tyr Asp Thr Glu Glu Glu Arg Ile
35 40 45
Asp Ser Val Asp Val Pro Ser Asn Ser Arg Gln Glu Glu Ala Asn Ala
50 55 60
Ile Ile Ala Asn Glu Ser Asp Ser Asp Pro Asp Asp Asp Leu Pro Leu
65 70 75 80
Ser Leu Val Arg Gln Arg Ala Ser Ala Ser Arg Gln Val Ser Gly Pro
85 90 95
Phe Tyr Thr Ser Lys Asp Gly Thr Lys Trp Tyr Lys Asn Cys Gln Arg
100 105 110
Pro Asn Val Arg Leu Arg Ser Glu Asn Ile Val Thr Glu Gln Ala Gln
115 120 125
Val Lys Asn Ile Ala Arg Asp Ala Ser Thr Glu Tyr Glu Cys Trp Asn
130 135 140
Ile Phe Val Thr Ser Asp Met Leu Gln Glu Ile Leu Thr His Thr Asn
145 150 155 160
Ser Ser Ile Arg His Arg Gln Thr Lys Thr Ala Ala Glu Asn Ser Ser
165 170 175
Ala Glu Thr Ser Phe Tyr Met Gln Glu Thr Thr Leu Cys Glu Leu Lys
180 185 190
Ala Leu Ile Ala Leu Leu Tyr Leu Ala Gly Leu Ile Lys Ser Asn Arg
195 200 205
Gln Ser Leu Lys Asp Leu Trp Arg Thr Asp Gly Thr Gly Val Asp Ile
210 215 220
Phe Arg Thr Thr Met Ser Leu Gln Arg Phe Gln Phe Leu Gln Asn Asn
225 230 235 240
Ile Arg Phe Asp Asp Lys Ser Thr Arg Asp Glu Arg Lys Gln Thr Asp
245 250 255
Asn Met Ala Ala Phe Arg Ser Ile Phe Asp Gln Phe Val Gln Cys Cys
260 265 270
Gln Asn Ala Tyr Ser Pro Ser Glu Phe Leu Thr Ile Asp Glu Met Leu
275 280 285
Leu Ser Phe Arg Gly Arg Cys Leu Phe Arg Val Tyr Ile Pro Asn Lys
290 295 300
Pro Ala Lys Tyr Gly Ile Lys Ile Leu Ala Leu Val Asp Ala Lys Asn
305 310 315 320
Phe Tyr Val Val Asn Leu Glu Val Tyr Ala Gly Lys Gln Pro Ser Gly
325 330 335
Pro Tyr Ala Val Ser Asn Arg Pro Phe Glu Val Val Glu Arg Leu Ile
340 345 350
Gln Pro Val Ala Arg Ser His Arg Asn Val Thr Phe Asp Asn Trp Phe
355 360 365
Thr Gly Tyr Glu Cys Met Leu His Leu Leu Asn Glu Tyr Arg Leu Thr
370 375 380
Ser Val Gly Thr Val Arg Lys Asn Lys Arg Gln Ile Pro Glu Ser Phe
385 390 395 400
Ile Arg Thr Asp Arg Gln Pro Asn Ser Ser Val Phe Gly Phe Gln Lys
405 410 415
Asp Ile Thr Leu Val Ser Tyr Ala Pro Lys Lys Asn Lys Val Val Val
420 425 430
Val Met Ser Thr Met His His Asp Asn Ser Ile Asp Glu Ser Thr Gly
435 440 445
Glu Lys Gln Lys Pro Glu Met Ile Thr Phe Tyr Asn Ser Thr Lys Ala
450 455 460
Gly Val Asp Val Val Asp Glu Leu Cys Ala Asn Tyr Asn Val Ser Arg
465 470 475 480
Asn Ser Lys Arg Trp Pro Met Thr Leu Phe Tyr Gly Val Leu Asn Met
485 490 495
Ala Ala Ile Asn Ala Cys Ile Ile Tyr Arg Thr Asn Lys Asn Val Thr
500 505 510
Ile Lys Arg Thr Glu Phe Ile Arg Ser Leu Gly Leu Ser Met Ile Tyr
515 520 525
Glu His Leu His Ser Arg Asn Lys Lys Lys Asn Ile Pro Thr Tyr Leu
530 535 540
Arg Gln Arg Ile Glu Met Gln Leu Gly Glu Pro Ser Pro Arg His Val
545 550 555 560
Asn Val Pro Gly Arg Tyr Val Arg Cys Gln Asp Cys Pro Tyr Lys Lys
565 570 575
Asp Arg Lys Thr Lys Arg Ser Cys Asn Ala Cys Ala Lys Pro Ile Cys
580 585 590
Met Glu His Ala Lys Phe Leu Cys Glu Asn Cys Ala Glu Leu Lys Ser
595 600 605
Ser Leu
610
<210> 87
<211> 1296
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 87
cagcaggtgg gccgcctact gcgcacgcgc gggtttgcgg gcagccgcct gggctgtggg 60
agcagcccgg gcagagctct cctgcctctc caccagccca ccccgccgcc tgaccgcccc 120
ctccccaccc cccacccccc acccccggaa aacgcgtcgt cccctgggct gggtggagac 180
ccccgtcccg cgaaacaccg ggccccgcgc agcgtccggg cctgacaccg ctccggcggc 240
tcgcctccta tgcgcccccg cgccaccgtc gcccgcccgc ccgggcccct gcagccgccc 300
aggtgccagc acggagcgcc tggcggcgga acgcagaccc caggcccggc gcacaccggg 360
gacgctgagc gttccaggcg ggagggaagg cgggcagaga tggagagagg aacgggagac 420
ctagaggggc ggaaggacgg gcggagggac gttaggaggg agggagggag gcagggaggc 480
agggaggaac ggagggaaag acagagcgac gcagggactg ggggcgggcg ggagggagcc 540
ggggaacggg gggaggaagg cagggaggaa aagcggtcct cggcctccgg gagtagcggg 600
acccccgccc tccgggaaaa cggtcagcgt ccggcgcggg ctgagggctg ggcccacagc 660
cgccgcgccg gccggcgggg caccacccat tcgccccggt tccgtggccc agggagtggg 720
cggtttcctc cgggacaaaa gaccgggact cgggttgccg tcgggtcttc acccgcgcgg 780
ttcacagacc gcacatcccc aggctgagcc ctgcaacgcg gcgcgaggcc gacagccccg 840
gccacggagg agccacacgc aggacgacgg aggcgtgatt ttggtttccg cgtggctttg 900
ccctccgcaa ggcggcctgt tgctcacgtc tctccggccc ccgaaaggct ggccatgccg 960
actgtttgct cccggagctc tgcgggcacc cggaaacatg cagggaaggg tgcaagcccg 1020
gcacggtgcc ttcgctctcc ttgccaggtt ccaaaccggc cacactgcag actccccacg 1080
ttgccgcacg cgggaatcca tcgtcaggcc atcacgccgg ggaggcatct cctctctggg 1140
gtctcgctct ggtcttctac gtggaaatga acgagagcca cacgcctgcg tgtgcgagac 1200
cgtcccggca acggcgacgc ccacaggcat tgcctccttc acggagagag ggcctggcac 1260
actcaagact cccacggagg ttcagttcca cactcc 1296
<210> 88
<211> 1296
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 88
caccaggtgg gccgcctact gcgcacgcgc gggtttgcgg gcagccgcct gggctgtggg 60
agcagcccgg gcagagctct cctgcctctc caccagccca ccccgccgcc tgaccgcccc 120
ctccccaccc cccacccccc acccccggaa aacgcgtcgt cccctgggct gggtggtgac 180
ccccgtcccg cgaaacaccg ggccccgcgc agcgtccggg cctgacaccg ctccggcggc 240
tcgcctccta tgcgcccccg cgccaccgtc gcccgcccgc ccgggcccct gcagccgccc 300
aggtgccagc acggagcgcc tggcggcgga acgcagaccc caggcccggc gcacaccggg 360
gacgctgagc gttccaggcg ggagggaagg cgggcagaga tggagagagg aacgggagtc 420
ctagaggggc ggaaggacgg gcggagggac gttaggaggg agggagggag gcagggaggc 480
agggaggaac ggagggaaag acagagcgac gcagggactg ggggcgggcg ggagggagcc 540
ggggaacggg gggaggaagg cagggaggaa aagcggtcct cggcctccgg gagtagcggg 600
acccccgccc tccgggaaaa cggtcagcgt ccggcgcggg ctgagggctg ggcccacagc 660
cgccgcgccg gccggcgggg caccacccat tcgccccggt tccgtggccc agggagtggg 720
cggtttcctc cgggacaaaa gaccgggact cgggttgccg tcgggtgttc acccgcgcgg 780
ttcacagacc gcacatcccc aggctgagcc ctgcaacgcg gcgcgaggcc gacagccccg 840
gccacggagg agccacacgc aggacgacgg aggcgtgatt ttggtttccg cgtggctttg 900
ccctccgcaa ggcggcctgt tgctcaagtc tctccggccc ccgaaaggct ggccatgccg 960
actgtttgct cccggagctc tgcgggcacc cggaaacatg cagggaaggg tgcaagcccg 1020
gcacggtgcc ttcgctctcc ttgccaggtt ccaaaccggc cacactgcag actccccacg 1080
ttgccgcacg cgggaatcca tcgtcaggcc atcacgccgg ggaggcatct cctctctggg 1140
gtgtcgctct ggacttctac gtggaaatga acgagagcca cacgcctgcg tgtgccagac 1200
cgtcccggca acggcgacgc ccacaggcat tgcctccttc acggagagag ggcctggcac 1260
actcaagact cccacggagg ttcagttcca cactcc 1296
<210> 89
<211> 433
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 89
ggcgggaggg aaggcgggca gagatggaga gaggaacggg agacctagag gggcggaagg 60
acgggcggag ggacgttagg agggagggag ggaggcaggg aggcagggag gaacggaggg 120
aaagacagag cgacgcaggg actgggggcg ggcgggaggg agccggggaa cggggggagg 180
aaggcaggga ggaaaagcgg tcctcggcct ccgggagtag cgggaccccc gccctccggg 240
aaaacggtca gcgtccggcg cgggctgagg gctgggccca cagccgccgc gccggccggc 300
ggggcaccac ccattcgccc cggttccgtg gcccagggag tgggcggttt cctccgggac 360
aaaagaccgg gactcgggtt gccgtcgggt cttcacccgc gcggttcaca gaccgcacat 420
ccccaggctg agc 433
<210> 90
<211> 433
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 90
ggcgggaggg aaggcgggca gagatggaga gaggaacggg agtcctagag gggcggaagg 60
acgggcggag ggacgttagg agggagggag ggaggcaggg aggcagggag gaacggaggg 120
aaagacagag cgacgcaggg actgggggcg ggcgggaggg agccggggaa cggggggagg 180
aaggcaggga ggaaaagcgg tcctcggcct ccgggagtag cgggaccccc gccctccggg 240
aaaacggtca gcgtccggcg cgggctgagg gctgggccca cagccgccgc gccggccggc 300
ggggcaccac ccattcgccc cggttccgtg gcccagggag tgggcggttt cctccgggac 360
aaaagaccgg gactcgggtt gccgtcgggt gttcacccgc gcggttcaca gaccgcacat 420
ccccaggctg agc 433
<210> 91
<211> 65
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 91
agaggggcgg aagggacgtt aggagggagg cagggaggca gggaggcagg gaggaacgga 60
gggag 65
<210> 92
<211> 1213
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 92
gcgagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc 60
cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat 120
ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt 180
tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa 240
aagctttagg ctgaaagaga gatttagaat gacagaatca tagaacggcc tgggttgcaa 300
aggagcacag tgctcatcca gatccaaccc cctgctatgt gcagggtcat caaccagcag 360
cccaggctgc ccagagccac atccagcctg gccttgaatg cctgcaggga tggggcatcc 420
acagcctcct tgggcaacct gttcagtgcg tcaccaccct ctgggggaaa aactgcctcc 480
tcatatccaa cccaaacctc ccctgtctca gtgtaaagcc attccccctt gtcctatcaa 540
gggggagttt gctgtgacat tgttggtctg gggtgacaca tgtttgccaa ttcagtgcat 600
cacggagagg cagatcttgg ggataaggaa gtgcaggaca gcatggacgt gggacatgct 660
ggtgttgagg gctctgggac actctccaag tcacagcgtt cagaacagcc ttaaggataa 720
gaagatagga tagaaggaca aagagcaagt taaaacccag catggagagg agcacaaaaa 780
ggccacagac actgctggtc cctgtgtctg agcctgcatg tttgatggtg tctggatgca 840
agcagaaggg gtggaagtgc ttgcctggag agatacagct gggtcagtag gactgggaca 900
ggcagctgga gaattgccat gtagatgttc atacaatcgt caaatcatga aggctggaaa 960
agccctccaa gatccccaag accaacccca acccacccac cgtgcccact ggccatgtcc 1020
ctcagtgcca catccccaca gttcttcatc acctccaggg acggtgaccc ccccacctcc 1080
gtgggcagct gtgccactgc agcaccgctc tttggagaag gtaaatcttg ctaaatccag 1140
cccgaccctc ccctggcaca acgtaaggcc attatctctc atccaactcc aggacggagt 1200
cagtgagaat att 1213
<210> 93
<211> 246
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 93
gcgagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc 60
cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat 120
ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt 180
tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa 240
aagctt 246
<210> 94
<211> 502
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 94
cctctcccgg ccagaggagc aaggtatgcg ggaggcgacc aggaggatag cggggctgac 60
gtcgggaggt ggcctccgtg ggaaggacac ccggatcttg acacagcctt ggcagcggag 120
tcaggaagag taggggtagg ttctggacgc cctcttggcc agctcatcgc cgccccaccc 180
tctgctggag cacagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc 240
cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg 300
aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg 360
gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga 420
aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag 480
tgcggtagtc gccttggacg tt 502
<210> 95
<211> 294
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 95
aagtttccag agctttcgag gaaggtttct tcaactcaaa ttcatccgcc tgataatttt 60
cttatatttt cctaaagaag gaagagaagc gcatagagga gaagggaaat aattttttag 120
gagcctttct tacggctatg aggaatttgg ggctcagttg aaaagcctaa actgcctctc 180
gggaggttgg gcgcggcgaa ctactttcag cggcgcacgc agacggcgtc tacgtgaggg 240
gtgataagtg acgcaacact cgttgcataa atttgcgctc cgccagcccg gagc 294
<210> 96
<211> 455
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 96
caacctttgg agctaagcca gcaatggtag agggaagatt ctgcacgtcc cttccaggcg 60
gcctccccgt caccaccccc cccaacccgc cccgaccgga gctgagagta attcatacaa 120
aaggactcgc ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag 180
aacccagaga tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg 240
gagaatagca tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 300
tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg 360
gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 420
aaccgtatat aagtgcagta gtcgccgtga acgtt 455
<210> 97
<211> 554
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 97
ggagacgatg acgtcgagga gaagttcccc aactttcccg cctctcagcc tttgaaagaa 60
agaaagggga gggggcaggc cgcgtgcagc cgcgagcggt gctgggctcc ggctccaatt 120
ccccatctca gtcgttccca aagtcctcct gtttcatcca agcgtgtaag ggtccccgtc 180
cttgactccc tagtgtcctg ctgcccacag tccagtcctg ggaaccagca ccgatcacct 240
cccatcgggc caatctcagt cccttccccc ctacgtcggg gcccacacgc tcggtgcgtg 300
cccagttgaa ccaggcggct gcggaaaaaa aaaagcgggg agaaagtagg gcccggctac 360
tagcggtttt acgggcgcac gtagctcagg cctcaagacc ttgggctggg actggctgag 420
cctggcggga ggcggggtcc gagtcaccgc ctgccgccgc gcccccggtt tctataaatt 480
gagcccgcag cctcccgctt cgctctctgc tcctcctgtt cgacagtcag ccgcatcttc 540
ttttgcgtcg ccag 554
<210> 98
<211> 646
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 98
ggagacgcga agaacaacga gaagatcctc aacttctcct aagccttttc actaataggg 60
agaagttcga tggggcagcc ttgggcagac ccacacttct gctccatttc cctggttcct 120
gcagctctca gattctccca ttttattcgg gaagcagctt tctggtttct gggtcctgga 180
tgtccttggt gcacactcca aggactcctc gtccttaatc catagtctgt attccctgag 240
tcctatcctg ggaaccctca tccggtcact tcctcggcgg gacaatctca gctcccctcc 300
ccctctcagg tcggagccca cacgcttggt gcgtgcacat ttcaaaaacg aggcgggtcc 360
aaaaagaggg agggggggaa tgagagaggc ccagctactc gcggctttac gggtgcacgt 420
agctcaggcc tctgcgccct tgagctggga ctggatgagc cgagcgggag gcggggcgcg 480
cgtcatcagc tccccccacc atccagttcc tataaatacg gactgcagcc ctccctggtg 540
ctctctgctc ctccctgttc tagagacagc cgcatcttct tgtgcagtgc caggctctct 600
gctcctcctg ttcgacagtc agccgcatct tcttttgcgt cgccag 646
<210> 99
<211> 218
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 99
gggaatgaga gaggcccagc tactcgcggc tttacgggtg cacgtagctc aggcctctgc 60
gcccttgagc tgggactgga tgagccgagc gggaggcggg gcgcgcgtca tcagctcccc 120
ccaccatcca gttcctataa atacggactg cagccctccc tggtgctctc tgctcctccc 180
tgttctagag acagccgcat cttcttgtgc agtgccag 218
<210> 100
<211> 519
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 100
cgaagaacaa cgaggagaag atcctcaact tttccgcagc cttttcaata atggggagag 60
gttcgatgat gcagtggcag ggagacccac acttctccat ttcccctgtt ctcccatttt 120
actcgggaag cagcattcag gtctctgggt cctggatgtc cttggtgcac actccaagga 180
ctcctcgtcc ttaagttcat agtctgtatt ccctgagtcc tatcctggga accatcaccc 240
ggtcacctcc tgagcggggc aatctcagct cccctccccc tatcagttcg gagcccacac 300
gcttggtgcg tgcacatttc aaaaatgagg cgggtccaaa gagagggagg aggggaaatg 360
agagaggccc agctactcgc ggctttacgg gtgcacgtag ctcaggcctc tgcgcccttg 420
agctaggact ggataagcag ggcgggaggc ggggcgcgcg tcatcagctc ccccccacca 480
tccgggttcc tataaatacg gactgcagcc ctccctggt 519
<210> 101
<211> 215
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 101
acgctcggtg cgtgcccagt tgaaccaggc ggctgcggaa aaaaaaaagc ggggagaaag 60
tagggcccgg ctactagcgg ttttacgggc gcacgtagct caggcctcaa gaccttgggc 120
tgggactggc tgagcctggc gggaggcggg gtccgagtca ccgcctgccg ccgcgccccc 180
ggtttctata aattgagccc gcagcctccc gcttc 215
<210> 102
<211> 103
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 102
ccttgggctg ggactggctg agcctggcgg gaggcggggt ccgagtcacc gcctgccgcc 60
gcgcccccgg tttctataaa ttgagcccgc agcctcccgc ttc 103
<210> 103
<211> 353
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 103
tcctatcctg ggaaccctca tccggtcact tcctcggcgg gacaatctca gctcccctcc 60
ccctctcagg tcggagccca cacgcttggt gcgtgcacat ttcaaaaacg aggcgggtcc 120
aaaaagaggg agggggggaa tgagagaggc ccagctactc gcggctttac gggtgcacgt 180
agctcaggcc tctgcgccct tgagctggga ctggatgagc cgagcgggag gcggggcgcg 240
cgtcatcagc tccccccacc atccagttcc tataaatacg gactgcagcc ctccctggtg 300
ctctctgctc ctccctgttc tagagacagc cgcatcttct tgtgcagtgc cag 353
<210> 104
<211> 151
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 104
agctgggact ggatgagccg agcgggaggc ggggcgcgcg tcatcagctc cccccaccat 60
ccagttccta taaatacgga ctgcagccct ccctggtgct ctctgctcct ccctgttcta 120
gagacagccg catcttcttg tgcagtgcca g 151
<210> 105
<211> 99
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 105
agctaggact ggataagcag ggcgggaggc ggggcgcgcg tcatcagctc ccccccacca 60
tccgggttcc tataaatacg gactgcagcc ctccctggt 99
<210> 106
<211> 524
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 106
atcctcaact tttccacagc ctttgcataa aggggagagg gtcggcggtg cagctgtggc 60
acacacgcac ttctgctcaa cccgcccccc cccgcccccg ttcctgttcc ttcccaggtt 120
ctccccattt tatcggggcg gcaactttta ggtccctggg tcctggaagt ccttagtaca 180
cactcttcgt ccttaagtcc atagtctgta ttccctcggt cctatcctgt cccccatcac 240
cgggtcacct ccccagcgaa gcaatctcag ttcccctccc cctctcagcc ccgagcccac 300
acgtttggtg cgtgcacatt tcaaaaacga ggcgggtcca aagagagggg gtggggaggt 360
gccgagtggc ccagctactc gcggctttac gggtgcacgt agctcaggcc tcagcgccct 420
tgagctgtga ctggatggat gagcggggcg ggaggcgggg cgagcgtcct cggcgctccc 480
caccacccca gttcctataa atacggactg cagccctccc cggt 524
<210> 107
<211> 497
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 107
ttcctcagct tgcccgcctc ccagcctttg aaagaatagg ggaagggggt ggcgcgtgct 60
gtccccaggc gaccgggctc aggctccgac tccccatgcc agccgctccc gggtcgtccg 120
tgcggcccct tggcgcggcc tgggctcctg gacctctctg gttcccacca ggatccccat 180
ccccgagtct atagtggctt gcgtgcccat agtcccgtcc cgggaacctt tagccatcac 240
tgcccccgcg ggccacctcg gtcccctccc cctctcaggc ctgggcccac atgcctggtg 300
cgtgcactgg ggaacaaggc gggcccgcaa aaagaaaaac gaggaggccc ggctactcgc 360
gggtttacgg gcgcacgtag ctcaggcctc ctcgcccttg ggctgggact gggcgagcag 420
cacgggaggc ggggcgcacg tcacccacgc cccgccgccc ccagtcccta taaattgagg 480
ctgcgggttc ctccggt 497
<210> 108
<211> 415
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 108
ggagacgggg tccgaatttc aaagtccttt ttattgactt acaaggtttt caaggaaaat 60
cttggaagta actgtgttcc gaagaatcta cgtttaaaaa ccgacccctg gatctttgcc 120
ttgggtccaa ggaccgagct ggccacgccc cagccgcgcc gcagccactc ccaaggcagt 180
tcaagtgtta agcccgaaag gtagagctct gcgcatgtgc acacccgtcc atagctgggt 240
cccagccaac caggccggag gagcacccgc gccgtcacgt gacgtgccca accggcgtcg 300
acctataaaa ggccgggcgt tgacgtcagc ggactcttcc gccgcagccg ccgccatcgt 360
cggcgcgctt ccctgttcac ctctgtattt gagaatccga cgccatctgc cacca 415
<210> 109
<211> 416
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 109
ggagacgcgg tccgaatttc aaagtctttt tcctattgac ctacaaggtt ttcaagaatc 60
atgttgtaag caactgtgtt ctgaggaatc tatgtttaaa aacccatccg tggatcttgg 120
cccagggtcc agagactgag ctagccacgc cccggccgcg ccgcagccac tcccacggca 180
gttcaagtgt taagtcccaa agaccgcgct ctgtgcatgc gcagacccgt ccacagctgg 240
ctcctagcca acccggccgg acgagcaccc ggcgccgtca cgtgacgcac ccaaccggcg 300
tcgacctata aaaggccggg cgttgacgtc agcgttctct tccgccgcag ccgccgccat 360
cgtcggcgcg cttccctgtt cacctctgac tctgagaatc cgtcgccatc cgccag 416
<210> 110
<211> 147
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 110
ggagacgttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 60
ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 120
taggcttttg caaaaagctc gtctcag 147
<210> 111
<211> 424
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 111
ggagacgcag ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg 60
aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt 120
cattggcgaa aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca cttcgcatat 180
taaggtgacg cgtgtggcct cgaacacaga gcgactctgc agggacacaa gacaggcttg 240
cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga 300
cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc 360
tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcactcgtc 420
tcag 424
<210> 112
<211> 226
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 112
ggagacggca catcgcccac agtccccgag aagttgggag gggtcggcgg atccggcccc 60
gcccagcgtc ttgtcattgg cgtattcgaa cacgcagatg cagtcggggc ggcgcggtcc 120
gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag 180
cgacccgctt aacagcgtca acagcgtgcc gcagatctcg tctcag 226
<210> 113
<211> 326
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 113
ggagacggca catcgcccac agtccccgag aagttgggag gggtcggcca gtgtggtttt 60
caagaggaag caaaaagcct ctccacccag gcctggaatg tttccaccca atgtcgagca 120
gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca ggcctggaat gtttccaccc 180
aatgtcgagc aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag 240
tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac 300
accgagcgac cctgcaggcg tctcag 326
<210> 114
<211> 1367
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 114
ggagacgcgg tccgaatttc aaagtctttt tcctattgac ctacaaggtt ttcaagaatc 60
atgttgtaag caactgtgtt ctgaggaatc tatgtttaaa aacccatccg tggatcttgg 120
cccagggtcc agagactgag ctagccacgc cccggccgcg ccgcagccac tcccacggca 180
gttcaagtgt taagtcccaa agaccgcgct ctgtgcatgc gcagacccgt ccacagctgg 240
ctcctagcca acccggccgg acgagcaccc ggcgccgtca cgtgacgcac ccaaccggcg 300
tcgacctata aaaggccggg cgttgacgtc agcgttctct tccgccgcag ccgccgccat 360
cgtcggcgcg cttccctgtt cacctctgac tctgagaatc cgtcgccatc cgccaggtga 420
gtctcctcgg ctccgctaga ctcggggacc gagaggaatc tctggggcag cgggacgtgg 480
ctgtagcggg acgctgagag ggacgggagg aagagacatg gctgccctgg cccgggcggc 540
aggacgtggt cgggccgcgg cgccatatct gcgcgtccct gagggccttg ggagtgtcaa 600
ctgccgaggt cggggtgttt tcttgaagtc cttcaactcc ccgcggccgc cggggtgact 660
gcgggagggg ttgtgcttgg tgatgtggca gcgggcaaag cgccgtcccc gcgcccctgg 720
tgacgggcgg agggtgtcct cgggaggtga cagcctgtag ggctggcttc cttggacacc 780
tccagtgggc tgaacgcctt ccgggccctt tccggtagcc cccgtgtctg ttttctatct 840
gagttcacac gtgagcaccg gtccccataa tctaagaaag tggctcactg ggcctagtgg 900
cgcattgtgg cctttgatcc gggctttgac cttggcgcac agcacccagt ggtttgggga 960
agaggtgtgt gtagcagagg aggttttttc gtgctttggt cccaatcaat ccggcatctt 1020
tgcagtgccg aggtggccgt gcaccttggc tttgaattct tgtgctgagg ttatgtgact 1080
tgagcctcaa gatagggtgt tctagcacag gcttgctctt aagtgtcgca gttgtcggtt 1140
tcggcgtttg tttagagctg tggacacatc tgtgaacttt tgatgcttat ttcagaggtc 1200
ctgggttgta cgtttgagtc acactgtgag ctcagctcca atcttgggcc gacatctggt 1260
tcctgcccct gctgtggggt gctattgacc caccgatgcc tgccaagttg ggttcccaga 1320
atcagcctgg ctgcccatcc ccccaccaca ggtgaacttc gtctcag 1367
<210> 115
<211> 457
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 115
ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 60
gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 120
taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 180
agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 240
agcacgactc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 300
cctttggggc agcggccaat agcagctttg ctccttcgct ttctgagagc agcggccggg 360
aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc 420
ggtgttccgc attctgcaag cctccggagc gcacgtc 457
<210> 116
<211> 507
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 116
ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 60
gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 120
taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 180
agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 240
agcacgactc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 300
cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 360
aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 420
gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 480
ctcttcctca tctccgggcc tttcgtc 507
<210> 117
<211> 460
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 117
ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 60
gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 120
taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 180
agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 240
agcacgactc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 300
cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg 360
cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 420
ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgtc 460
<210> 118
<211> 466
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 118
cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct 60
ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gcctcgcaca ttcttcacgt 120
ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc 180
ctcgtccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa 240
cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag 300
cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca 360
gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc 420
tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtc 466
<210> 119
<211> 215
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 119
cagctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat 60
atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc 120
gaaaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca tattaaggtg 180
acgcgtgtgg cctcgaacac agagcgactc tgcag 215
<210> 120
<211> 753
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 120
aaatgagtct tcggacctcg cgggggccgc ttaagcggtg gttagggttt gtctgacgcg 60
gggggagggg gaaggaacga aacactctca ttcggaggcg gctcggggtt tggtcttggt 120
ggccacgggc acgcagaaga gcgccgcgat cctcttaagc acccccccgc cctccgtgga 180
ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct gactcgggcg ggtcgcgcgc 240
cccagagtgt gaccttttcg gtctgctcgc agacccccgg gcggcgccgc cgcggcggcg 300
acgggctcgc tgggtcctag gctccatggg gaccgtatac gtggacaggc tctggagcat 360
ccgcacgact gcggtgatat taccggagac cttctgcggg acgagccggg tcacgcggct 420
gacgcggagc gtccgttggg cgacaaacac caggacgggg cacaggtaca ctatcttgtc 480
acccggaggc gcgagggact gcaggagctt cagggagtgg cgcagctgct tcatccccgt 540
ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag 600
ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat 660
gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc gtgtggcctc 720
gaacaccgag cgaccctgca gcgacccgct taa 753
<210> 121
<211> 270
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 121
cagtgtggtt ttcaagagga agcaaaaagc ctctccaccc aggcctggaa tgtttccacc 60
caatgtcgag cagtgtggtt ttgcaagagg aagcaaaaag cctctccacc caggcctgga 120
atgtttccac ccaatgtcga gcaaaccccg cccagcgtct tgtcattggc gaattcgaac 180
acgcagatgc agtcggggcg gcgcggtccc aggtccactt cgcatattaa ggtgacgcgt 240
gtggcctcga acaccgagcg accctgcagg 270
<210> 122
<211> 149
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 122
tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 60
ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 120
gcttttttgg aggtataggc ttttgcaaa 149
<210> 123
<211> 128
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 123
gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 60
ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 120
tttgcaaa 128
<210> 124
<211> 408
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 124
cagctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat 60
atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc 120
gaaaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca tattaaggtg 180
acgcgtgtgg cctcgaacac agagcgactc tgcagggaca caagacaggc ttgcgagata 240
tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac 300
tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc 360
ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcac 408
<210> 125
<211> 332
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 125
ggcctccgcg ccgggttttg gcgccccccg cgggcgcccc ctcctcacgg cgagcgctgc 60
cacgtcagac gaagggcgca cgagcgtcct gatccttccg cccggacgct caggacagcg 120
gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga 180
cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa 240
aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga 300
ttatataagg acgcgccggg tgtggcacag ct 332
<210> 126
<211> 334
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 126
ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg 60
ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag 120
cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag 180
gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg 240
aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat 300
gattatataa ggacgcgccg ggtgtggcac agct 334
<210> 127
<211> 334
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 127
ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg 60
ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag 120
cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag 180
gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg 240
aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat 300
gattatataa ggacgcgccg ggtgtggcac agct 334
<210> 128
<211> 178
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 128
gcacatcgcc cacagtcccc gagaagttgg ggggaggctc tggctgcagg taattgaacc 60
ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 120
ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttc 178
<210> 129
<211> 369
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 129
acccgccccg accggagctg agagtaattc atacaaaagg actcgcccct gccttgggga 60
atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg 120
ccccctcacc cgcccgctct cgtcatcact gaggtggaga atagcatgcg tgaggctccg 180
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg ggaggggtcg 240
gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 300
actggctcag cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 360
tgaacgttc 369
<210> 130
<211> 369
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 130
acccgccccg accggagctg agagtaattc atacaaaagg actcgcccct gccttgggga 60
atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg 120
ccccctcacc cgcccgctct cgtcatcact gaggtggaga atagcatgcg tgaggctccg 180
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg ggaggggtcg 240
gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 300
actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 360
tgaacgttc 369
<210> 131
<211> 372
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 131
acccgccccg accggagctg agagtaattc atacaaaagg actcgcccct gccttgggga 60
atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg 120
ccccctcacc cgcccgctct cgtcatcact gaggtggaga atagcatgcg tgaggctccg 180
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg 240
tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg 300
tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg 360
ccgtgaacgt tc 372
<210> 132
<211> 372
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 132
acccgccccg accggagctg agagtaattc atacaaaagg actcgcccct gccttgggga 60
atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg 120
ccccctcacc cgcccgctct cgtcatcact gaggtggaga atagcatgcg tgaggctccg 180
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg 240
tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg 300
tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg 360
ccgtgaacgt tc 372
<210> 133
<211> 372
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 133
acccgccccg accggagctg agagtaattc atacaaaagg actcgcccct gccttgggga 60
atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg 120
ccccctcacc cgcccgctct cgtcatcact gaggtggaga atagcatgcg tgaggctccg 180
gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg 240
tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg 300
tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg 360
ccgtgaacgt tc 372
<210> 134
<211> 570
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 134
ctgggctgag acccgcagag gaagacgctc tagggatttg tcccggacta gcgagatggc 60
aaggctgagg acgggaggct gattgagagg cgaaggtaca ccctaatctc aatacaacct 120
ttggagctaa gccagcaatg gtagagggaa gattctgcac gtcccttcca ggcggcctcc 180
ccgtcaccac cccccccaac ccgccccgac cggagctgag agtaattcat acaaaaggac 240
tcgcccctgc cttggggaat cccagggacc gtcgttaaac tcccactaac gtagaaccca 300
gagatcgctg cgttcccgcc ccctcacccg cccgctctcg tcatcactga ggtggagaag 360
agcatgcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 420
agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa 480
actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 540
atataagtgc agtagtcgcc gtgaacgttc 570
<210> 135
<211> 168
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 135
gcacatcgcc cacagtcccc gagaagttgg gaggggtcgg caattgaacc ggtgcctaga 60
gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 120
agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttc 168
<210> 136
<211> 171
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 136
gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 60
agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 120
ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt c 171
<210> 137
<211> 171
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 137
gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 60
agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 120
ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt c 171
<210> 138
<211> 168
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 138
gcacatcgcc cacagtcccc gagaagttgg gaggggtcgg caattgaacc ggtgcctaga 60
gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctcagc ctttttcccg 120
agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttc 168
<210> 139
<211> 171
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 139
gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct 60
agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 120
ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt c 171
<210> 140
<211> 440
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 140
agcggggctg acgtcgggag gtggcctccg tgggaaggga cacccggatc ttgacacagc 60
cttggcagcg gagtaaggaa gagtagggat agattctggc cgccctcttg gccagcttct 120
cgccgcccca ccctccgcta gggccaagag taattcatac aaaaggaggg atcgccttcg 180
caaggggaga gcccagggac cgtccctaaa ttctcacaga cccaaatccc tgtagccgcc 240
ccacgacagc gcgaggagca tgcgctcagg gctgagcgcg gggagagcag agcacacaag 300
ctcatagacc ctggtcgtgg gggggaggac cggggagctg gcgcggggca aactgggaaa 360
gcggtgtcgt gtgctggctc cgccctcttc ccgagggtgg gggagaacgg tatataagtg 420
cggcagtcgc cttggacgtt 440
<210> 141
<211> 480
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 141
agcggggctg acgtcgggag gtggcctccg tgggaaggga cacccggatc ttgacacagc 60
cttggcagcg gagtaaggaa gagtagggat agattctggc cgccctcttg gccagcttct 120
cgccgcccca ccctccgcta gggccaagag taattcatac aaaaggaggg atcgccttcg 180
cctggggaag tcccagggac cgtcgctaaa ttctcataac ccataatccc ggtacccgcc 240
ccaccacagt gcgaggagca tgcgctcagg gctgagcgcg gggagagcag agcacacaag 300
ctcatagacc ctggtcgtgg ggggaggggc gcactgagcg gggggggggg gggtgatggg 360
ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg tgctggctcc 420
gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc ttggacgttc 480
<210> 142
<211> 316
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 142
ggagccgaga gtaattcata caaaaggagg gatcgccttc gcaaggggag agcccaggga 60
ccgtccctaa attctcacag acccaaatcc ctgtagccgc cccacgacag cgcgaggagc 120
atgcgcccag ggctgagcgc gggtagatca gagcacacaa gctcacagtc cccggcggtg 180
gggggagggg cgcgctgagc gggggccagg gagctggcgc ggggcaaact gggaaagtgg 240
tgtcgtgtgc tggctccgcc ctcttcccga gggtggggga gaacggtata taagtgcggt 300
agtcgccttg gacgtt 316
<210> 143
<211> 503
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 143
cctctcccgg ccagaggagc aaggtatgcg ggaggcgacc aggaggatag cggggctgac 60
gtcgggaggt ggcctccgtg ggaaggacac ccggatcttg acacagcctt ggcagcggag 120
tcaggaagag taggggtagg ttctggacgc cctcttggcc agctcatcgc cgccccaccc 180
tctgctggag cacagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc 240
cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg 300
aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg 360
gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga 420
aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag 480
tgcggtagtc gccttggacg ttc 503
<210> 144
<211> 503
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 144
cctctcccgg ccagaggagc aaggtatgcg ggaggcgacc aggaggatag cggggctgac 60
gtcgggaggt ggcctccgtg ggaaggacac ccggatcttg acacagcctt ggcagcggag 120
tcaggaagag taggggtagg ttctggacgc cctcttggcc agctcttcgc cgccccaccc 180
tctgctggag cacagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc 240
cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg 300
aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg 360
gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga 420
aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag 480
tgcggtagtc gccttggacg ttc 503
<210> 145
<211> 301
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 145
ggagccgaga gtaattcata caaaaggagg gatcgccttc gcaaggggag agcccaggga 60
ccgtccctaa attctcacag acccaaatcc ctgtagccgc cccacgacag cgcgaggagc 120
atgcgctcag ggctgagcgc ggggagagca gagcacacaa gctcatagac cctggtcgtg 180
ggggggagga ccggggagct ggcgcggggc aaactgggaa agcggtgtcg tgtgctggct 240
ccgccctctt cccgagggtg ggggagaacg gtatataagt gcggcagtcg ccttggacgt 300
t 301
<210> 146
<211> 459
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 146
ggcggggctg acgtcgggag gtggcctcca cgggaaggga cacccggatc tcgacacagc 60
cttggcagtg gagtcaggaa gggtaggaca gattctggac gccctcttgg ccagtcctca 120
ccgccccacc cccgatggag ccgagagtaa ttcatacaaa aggagggatc gccttcgccc 180
ctgggaatcc cagggaccgt cgctaaattc tggccggcct cccagcccgg aaccgctgtg 240
cccgcccagc gcggcgggag gagcctgcgc ctagggcgga tcgcgggtcg gcgggagagc 300
acaagcccac agtccccggc ggtgggggag gggcgcgctg agcgggggcc cgggagccag 360
cgcggggcaa actgggaaag tggtgtcgtg tgctggctcc gccctcttcc cgagggtggg 420
ggagaacggt ataaaagtgc ggtagtcgcg ttggacgtt 459
<210> 147
<211> 783
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 147
tattaatagc aatcttagct aattaaaata gatagcgttt attgagcgtt gggtatcagg 60
cacggtccta attcttttag atgtctttag ttcgtttcac tctcccccaa acaatagggt 120
ggtattgatc acctccgagc aggtgaaatt gaggcacaga gaaatcctag tagctggtag 180
aagaacacgc agtgtggtca agctagcaag gtgtttggtc cactgctata tctacaaaac 240
ccctaacaat gcctggtgta tagatgctca gtatgcattt gtgggatcag tgattccgat 300
gcctgcttct tataaagttt ttatttagaa ataattacag gtaaggagtt gcaaaaacag 360
tatagtggga tcgagtgtcc tttttccctc ggcttctccc aggggtatcg tcttacgtaa 420
caatgtccaa acagggaaat tgacttgggt ataatccaca gactctattc accttgtaga 480
ttggttttaa tagaagtaac tggacaactt gtaagctaat atcgttgcta tggttctcgt 540
tctcagctaa aacggcgctc tttactttgt gcacctgaac actgcacacc gagggcgacc 600
accgcccccg agatgcccag cttctattct agagcgccgc gccggcgccg aatgggttaa 660
cgggcggggg gacacgcctc cgtgcgcttg cgcggcgtcc cttcgccccg ccttcgcagc 720
gcagtcacat gacccgccca accggcgtcc gcctataaaa agctgagtgt tgacgtcagc 780
gtt 783
<210> 148
<211> 335
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 148
ggatccaact tctaagtccg ttttttattg atctaaaagg ccttttgcga atcatcttga 60
aggcaatcgc gttctgagcc acctcagctt tggcacacag cgcggggact gtcgcgaggg 120
gtttagggcc caagcaggac acaccccgaa atctccgcag ccacccccac cccacgcccc 180
cggctcttga gggttaaatc gcaggcgcag gttctcgcac gcgcacatca tcccgcaggc 240
gagccccagc acccagccca gggtgcgcgc gcgccgtcac gtgacacgcc caaccggcgt 300
cgccgtataa aagcgcgggc gttgacgtca gcggt 335
<210> 149
<211> 335
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 149
ggatccaact tctaagtccg ttttttattg atctaaaagg ccttttgcga atcatcttga 60
aggcaatcgc gttctgagcc acctcagctt tggcacacag cgcggggact gtcgcgaggg 120
gtttagggcc caagcaggac acaccccgaa atctccgcag ccacccccac cccacgcccc 180
cggctcttga gggttaaatc gcaggcgctg gttctcgcac gcgcacatca tcccgcaggc 240
gagccccagc acccagccca gggtgcgcgc gcgccgtcac gtgacacgcc caaccggcgt 300
cgccgtataa aagcgcgggc gttgacgtca gcgtt 335
<210> 150
<211> 343
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 150
tgacttgggt ataatccaca gactctattc accttgtaga ttggttttaa tagaagtaac 60
tggacaactt gtaagctaat atcgttgcta tggttctcgt tctcagctaa aacggcgctc 120
tttactttgt gcacctgaac actgcacacc gagggcgacc accgcccccg agatgcccag 180
cttctattct agagcgccgc gccggcgccg aatgggttaa cgggcggggg gacacgcctc 240
cgtgcgcttg cgcggcgtcc cttcgccccg ccttcgcagc gcagtcacat gacccgccca 300
accggcgtcc gcctataaaa agctgagtgt tgacgtcagc gtt 343
<210> 151
<211> 329
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 151
cggtccgaat ttcaaagtct ttttcctatt gacctacaag gttttcaaga atcatgttgt 60
aagcaactgt gttctgagga atctatgttt aaaaacccat ccgtggatct tggcccaggg 120
tccagagact gagctagcca cgccccggcc gcgccgcagc cactcccacg gcagttcaag 180
tgttaagtcc caaagaccgc gctctgtgca tgcgcagacc cgtccacagc tggctcctag 240
ccaacccggc cggacgagca cccggcgccg tcacgtgacg cacccaaccg gcgtcgacct 300
ataaaaggcc gggcgttgac gtcagcggt 329
<210> 152
<211> 329
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 152
cggtccgaat ttcaaagtct ttttcctatt gacctacaag gttttcaaga atcatgttgt 60
aagcaactgt gttctgagga atctatgttt aaaaacccat ccgtggatct tggcccaggg 120
tccagagact gagctagcca cgccccggcc gcgccgcagc cactcccacg gcagttcaag 180
tgttaagtcc caaagaccgc gctctgtgca tgcgcagacc cgtccacagc tggctcctag 240
ccaacccggc cggacgagca cccggcgccg tcacgtgacg cacccaaccg gcgtcgacct 300
ataaaaggcc gggcgttgac gtcagcgtt 329
<210> 153
<211> 325
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 153
gggtccgaat ttcaaagtcc tttttattga cttacaaggt tttcaaggaa aatcttggaa 60
gtaactgtgt tccgaagaat ctacgtttaa aaaccgaccc ctggatcttt gccttgggtc 120
caaggaccga gctggccacg ccccagccgc gccgcagcca ctcccaaggc agttcaagtg 180
ttaagcccga aaggtagagc tctgcgcatg tgcacacccg tccatagctg ggtcccagcc 240
aaccaggccg gaggagcacc cgcgccgtca cgtgacgtgc ccaaccggcg tcgacctata 300
aaaggccggg cgttgacgtc agcgg 325
<210> 154
<211> 326
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 154
gggtccgaat ttcaaagtcc tttttattga cttacaaggt tttcaaggaa aatcttggaa 60
gtaactgtgt tccgaagaat ctacgtttaa aaaccgaccc ctggatcttt gccttgggtc 120
caaggaccga gctggccacg ccccagccgc gccgcagcca ctcccaaggc agttcaagtg 180
ttaagcccga aaggtagagc tctgcgcatg tgcacacccg tccatagctg ggtcccagcc 240
aaccaggccg gaggagcacc cgcgccgtca cgtgacgtgc ccaaccggcg tcgacctata 300
aaaggccggg cgttgacgtc agcgtt 326
<210> 155
<211> 944
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 155
gtgagtggcg ggtgtggctt ccgcgggccc cggagctgga gccctgctct gagcgggccg 60
ggctgatatg cgagtgtcgt ccgcagggtt tagctgtgag cattcccact tcgagtggcg 120
ggcggtgcgg gggtgagagt gcgaggccta gcggcaaccc cgtagcctcg cctcgtgtcc 180
ggcttgaggc ctagcgtggt gtccgccgcc gcgtgccact ccggccgcac tatgcgtttt 240
ttgtccttgc tgccctcgat tgccttccag cagcatgggc taacaaaggg agggtgtggg 300
gctcactctt aaggagccca tgaagcttac gttggatagg aatggaaggg caggaggggc 360
gactggggcc cgcccgcctt cggagcacat gtccgacgcc acctggatgg ggcgaggcct 420
gtggctttcc gaagcaatcg ggcgtgagtt tagcctacct gggccatgtg gccctagcac 480
tgggcacggt ctggcctggc ggtgccgcgt tcccttgcct cccaacaagg gtgaggccgt 540
cccgcccggc accagttgct tgcgcggaaa gatggccgct cccggggccc tgttgcaagg 600
agctcaaaat ggaggacgcg gcagcccggt ggagcgggcg ggtgagtcac ccacacaaag 660
gaagagggcc ttgcccctcg ccggccgctg cttcctgtga ccccgtggtc tatcggccgc 720
atagtcacct cgggcttctc ttgagcaccg ctcgtcgcgg cggggggagg ggatctaatg 780
gcgttggagt ttgttcacat ttggtgggtg gagactagtc aggccagcct ggcgctggaa 840
gtcattcttg gaatttgccc ctttgagttt ggagcgaggc taattctcaa gcctcttagc 900
ggttcaaagg tattttctaa acccgtttcc aggtgttgtg aaag 944
<210> 156
<211> 252
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 156
gtgaaaaaga aaagaaaaaa aaggactggg ccgcaggagg ccggagagga atggaaatta 60
ggaatggggg gaaggacgct gtacgggttt aggggcgctg gtgcgaggtc cggaagccga 120
gcccaggctc cgcattgcag aggatggtag aggacgtgat ggggcatgcg gcgggaatgg 180
aggcgggtgg ggggagggga ctggccacgc taatctgact ttcttctccc gcagcctctt 240
ctcatagaca ag 252
<210> 157
<211> 874
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 157
gtgagtgtcc gcggcgcggc aagacttggg gactgtgacg agacttcggg gcagcgggag 60
gtggccggag cgggacccgg aaaagaaagg agacatggct gcctctgcat gggtggcggg 120
acgtggtcgg ctcgcggcgc catatctgca cctcctctgc ccgtctttgg gagtgtcggc 180
ctcctgaagt tggagtgttt tctctaattc cttcgtccag ctctcctttc cgagaacgct 240
ggggtggctg tgggaggggc ggcgtttgct gatgtggcag cggacataat gctgtatagc 300
cctgtgccca tggtgacagg gtgatggtgc tcccgggaag tgacagcctg caggggtggc 360
tcacatggtg acctctagtg agctgagcct cttccgccct ggcctttatc tccttccttg 420
gtccgcacaa tggaaccggt cccctccaag ctgagaaaat ggctcatggg cctaggggcc 480
tattgtggcc tttgatccca gcatttgacc ttggcgcaca aggcgggttg gcagtgtgta 540
gcaggcgagg ttttgtcggc ctgtgtgggc cccatctcgt gcgggccccc tgtcgcctgc 600
attgttggac tgctggggtg gcagtccagc ttggcgttga ttacgtggtg cggtcacagc 660
ctaggctccc tggtactctt gttctagttg tcattttggt tagggttggg ttcctgacac 720
atctggtgac tcttgatgct tcttaggtgg taggcttgta ggtgtgagtc gaatgagcgc 780
cagttttggg gagacagctc tttggaaccc cacaatgggg tgctatcgac ccgagttccc 840
agaatcagtc ctgaccgccc ttcccccacc acag 874
<210> 158
<211> 292
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 158
gtaggctgag caccgtggcg ggcggcagcg ggtggcggtc ggggttgttt ctggcggagg 60
tgctgctgat gatgtaatta aagtaggcgg tcttgagagg gcggatggtc gaggtgaggt 120
gtggcaggct tgagatccag ctgttggggt gagtactccc tctcaaaagc gggcattact 180
tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgat 240
ctggccatac acttgagtga caatgacatc cactttgcct ttctctccac ag 292
<210> 159
<211> 701
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 159
gtactggccc acagccgtaa agagctgcgg gggcgtgaga ggggggaatg ggtgaggtca 60
agctggaggc ttcttggggt tgggtgggcc gctgagggga ggggagggcg aggtgacgcg 120
acacccggcc tttctgggag agtgggcctt gttgacctaa ggggggcgag ggcagttggc 180
acgcgcacgc gccgacagaa actaacagac attaaccaac agcgattccg tcgcgtttac 240
ttgggaggaa ggcggaaaag aggtagtttg tgtggcttct ggaaacccta aatttggaat 300
cccagtatga gaatggtgtc ccttcttgtg tttcaatggg atttttactt cgcgagtctt 360
gtgggtttgg ttttgttttc agtttgccta acaccgtgct taggtttgag gcagattgga 420
gttcggtcgg gggagtttga atatccggaa cagttagtgg ggaaagctgt ggacgcttgg 480
taagagagcg ctctggattt tccgctgttg acgttgaaac cttgaatgac gaatttcgta 540
ttaagtgact tagccttgta aaattgaggg gaggcttgcg gaatattaac gtatttaagg 600
cattttgaag gaatagttgc taattttgaa gaatattagg tgtaaaagca agaaatacaa 660
tgatcctgag gtgacacgct tatgttttac ttttaaacta g 701
<210> 160
<211> 280
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 160
tgtatactct atattatact ctatgttata ctctgtaatc ctactcaata aacgtgtcac 60
gcctgtgaaa ccgtactaag tctcccgtgt cttcttatca ccatcaggtg acatcctcgc 120
ccaggctgtc aatcatgccg gtatcgattc cagtagcacc ggccccacgc tgacaaccca 180
ctcttgcagc gttagcagcg cccctcttaa caagccgacc cccaccagcg tcgcggttac 240
taacactcct ctccccgggg catccgctac tcccgagctc 280
<210> 161
<211> 280
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 161
tgtatactct atattatact ctatgttata ctctgtaatc ctactcaata aacgtgtcac 60
gcctgtgaaa ccgtactaag tctcccgtga cttcttatca ccatcaggtg acatcctcgc 120
ccaggctgtc aatcatgccg gtatcgattc cagtagcacc ggccccacgc tgacaaccca 180
ctcttgcagc gttagcagcg cccctcttaa caagccgacc cccaccagcg tcgcggttac 240
taacactcct ctccccgggg catccgctac tcccgagctc 280
<210> 162
<211> 328
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 162
actattgtat atatatatca gttactgtta tggatcccac gtcactattg tatactctat 60
attatactct atgttatact ctgtaatcct actcaataaa cgtgtcacgc ctgtgaaacc 120
gtactaagtc tcccgtgtct tcttatcacc atcaggtgac atcctcgccc aggctgtcaa 180
tcatgccggt atcgattcca gtagcaccgg ccccacgctg acaacccact cttgcagcgt 240
tagcagcgcc cctcttaaca agccgacccc caccagcgtc gcggttacta acactcctct 300
ccccggggca tccgctactc ccgagctc 328
<210> 163
<211> 328
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 163
actattgtat atatatatca gttactgtta tggatcccac gtcactattg tatactctat 60
attatactct atgttatact ctgtaatcct actcaataaa cgtgtcacgc ctgtgaaacc 120
gtactaagtc tcccgtgtct tgttatcacc atcaggtgac atcctcgccc aggctgtcaa 180
tcatgccggt atcgattcca gtagcaccgg ccccacgctg acaacccact cttgcagcgt 240
tagcagcgcc cctcttaaca agccgacccc caccagcgtc gcggttacta acactcctct 300
ccccggggca tccgctactc ccgagctc 328
<210> 164
<211> 343
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 164
atccacaagc ccagctcccc acccatcacc atggacaatg tttttttact aacacttgga 60
caatgatgga tactttttta ctaacacttg gacaatgatg atgatacact cctcacctgc 120
ccacttagac acaattacta acaccacacc ccctctttta tttctctgta cttaatgttt 180
tctgaataaa gtgatcctat tgtacccaca ttaaagactt ctttaactct ttatggttca 240
caggacccga gatgaacata gatattgtta cagcagcggc ctccatgtca ggtataacta 300
ctgcctcaca cagcgccctg ccaatcagaa gaccaaacac ccc 343
<210> 165
<211> 331
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 165
atccacaagc ccagctcccc acccatcacc atggacaatg tttttttact aacacttgga 60
caatgatgga tactttttta ctaacacttg gacaatgatg atgatacact cctcacttgc 120
ccacttagac acaattacta acaccacacc ccctctttta tttctctgta cttaatgttt 180
tctgaataaa gtgatcctat tgtacccaca ttaaagactt ctttaactct ttatggttca 240
caggacccga gatgaacata gatattgtta cagcagcggc ctccatgtca ggtataacta 300
ctgcctcaca cagcgccctg ccaatcagaa g 331
<210> 166
<211> 270
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 166
atattacccc taacacctgc caccccagtc ttaatcagtg gtggaagaac ggtctcagaa 60
ctgtttgtct caattggcca tttaagttta atagtgaaag actggttaat gataacaatg 120
catcggaaaa ccttcaggag gaaaggagaa tgttttgtgg aacatttttg tgtgtgtggc 180
agttttaagt tattagtttt caaaatcagt actttttaat ggaaacaact tgaccaaaaa 240
tctgtcacag aattttgaga cccattaaaa 270
<210> 167
<211> 292
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 167
atattacccc taacacctgc caccccagtc ttaatcagtg gtggaagaac ggtgtcagaa 60
ctgtttgtct caattggcca tttaagttta atagtgaaag actggttaat gataacaatg 120
catcggaaaa ccttcaggag gaaaggagaa tgttttgtgg aacatttttg tgtgtgtggc 180
agttttaagt tattagtttt caaaatcagt actttttaat ggaaacaact tgaccaaaaa 240
tctgtcacag aatttttaga cccattaaaa tacaagttta atgagaagtc tg 292
<210> 168
<211> 573
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 168
attatcccta atacctgcca ccccactctt aatcagtggt ggaagaacgg tctcagaact 60
gtttgtttca attggccatt taagtttagt agtaaaagac tggttaatga taacaatgca 120
tcgtaaaacc ttcagaagga aaggagaatg ttttgtggac cactttggtt ttcttttttg 180
cgtgtggcag ttttaagtta ttagttttta aaatcagtac tttttaatgg aaacaacttg 240
accaaaaatt tgtcacagaa ttttgagacc cattaaaaaa gttaaatgag aaacctgtgt 300
gttcctttgg tcaacaccga gacatttagg tgaaagacat ctaattctgg ttttacgaat 360
ctggaaactt cttgaaaatg taattcttga gttaacactt ctgggtggag aatagggttg 420
ttttcccccc acataattgg aaggggaagg aatatcattt aaagctatgg gagggttgct 480
ttgattacaa cactggagag aaatgcagca tgttgctgat tgcctgtcac taaaacaggc 540
caaaaactga gtccttgggt tgcatagaaa gct 573
<210> 169
<211> 280
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 169
attatcccta atacctgcca ccccactctt aatcagtggt ggaagaacgg tgtcagaact 60
gtttgtttca attggccatt taagtttagt agtaaaagac tggttaatga taacaatgca 120
tcgtaaaacc ttcagaagga aaggagaatg ttttgtggac cactttggtt ttcttttttg 180
cgtgtggcag ttttaagtta ttagttttta aaatcagtac tttttaatgg aaacaacttg 240
accaaaaatt tgtcacagaa ttttgacacc cattaaaaaa 280
<210> 170
<211> 186
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 170
gcatatactg agattgagat taacttcctg tgaaacccag tgtcttagac aactgtggct 60
tgagcaccac ctgctggtat tcattacaaa cttgctcact acaataaatg aattttaagc 120
tttaagatga agtggcattt cttttaacag ttactatgtt ggaattggtt acaaattttg 180
gagtgg 186
<210> 171
<211> 186
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 171
gcatatactg agattgagat taacttcctg tgaaacccag tgtcttagac aactgtggct 60
tgagcaccac ctgttggtat tcattacaaa cttgctcact acaataaatg aattttaagc 120
tttaagatga agtggcattt cttttaacag ttactatgtt ggaattggtt acaaattttg 180
gagtgg 186
<210> 172
<211> 337
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 172
atattatccc taatacttgc caccccactc ttaatcagtg gtggaagaac ggtgtcagaa 60
ctgtttgttt caattggcca tttaagttta gtagtaaaag actggttaat gataacaatg 120
catcgtaaaa ccttcagaag gaaaggagaa tgttttgtgg accactttgg ttttcttttt 180
tgcgtgtggc agttttaagt tattagtttt taaaatcagt actttttaat ggaaacaact 240
tgaccaaaaa tttgtcacag aattttgaga tccattaaaa aagttaaatg agaaacctgt 300
gtgttccttt ggtcaacacc gagacattta ggtgaaa 337
<210> 173
<211> 185
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 173
ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 60
tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 120
ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggc aagacaatag 180
caggc 185
<210> 174
<211> 251
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 174
ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 60
tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 120
ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aatacaatag 180
caggcatgct ggggatgcgg tgggctctat gggtacccag gtgctgaaga attgacccgg 240
ttcctcctgg g 251
<210> 175
<211> 99
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 175
ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 60
tcccactgtc ctttcctaat aaaatgagga aattgcatc 99
<210> 176
<211> 479
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 176
acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc 60
cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt 120
ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga 180
caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt 240
ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt 300
tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac 360
ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac 420
cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtcctt 479
<210> 177
<211> 202
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 177
ctgcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 60
actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 120
gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcccaagttg 180
ggaagaaacc tgtagggcct gc 202
<210> 178
<211> 210
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 178
ctcgctttct tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact 60
aaactggggg atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt 120
attttcattg caatgatgta tttaaattat ttctgaatat tttactaaaa agggaatgtg 180
ggaggtcagt gcatttaaaa cataaagaaa 210
<210> 179
<211> 210
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 179
ctcgctttct tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact 60
aaactggggg atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt 120
attttcattg caatgatgta tttaaattat ttctgaatat tttactaaaa agggaatgtg 180
ggagatcagt gcatttaaaa cataaagaaa 210
<210> 180
<211> 387
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 180
tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc 60
actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta 120
gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg 180
tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac 240
ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa 300
attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat 360
agctgtccct cttctcttat ggagatc 387
<210> 181
<211> 527
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 181
ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg 60
gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag 120
ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 180
tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 240
cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac 300
aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta 360
ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt 420
ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc 480
tcctgactac tcccagtcat agctgtccct cttctcttat ggagatc 527
<210> 182
<211> 387
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 182
tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc 60
actcggaaga acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta 120
gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg 180
tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac 240
ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa 300
attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat 360
agctgtccct cttctcttat ggagatc 387
<210> 183
<211> 387
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 183
aggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc 60
actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta 120
gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg 180
tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac 240
ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa 300
attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat 360
agctgtccct cttctcttat ggagatc 387
<210> 184
<211> 99
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 184
gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 60
tctctcactc ggaaggacat atgggagggc aaatcattt 99
<210> 185
<211> 155
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 185
gtgcgacggc cggcaagccc ccgctccccg ggctctcgcg gtcgcacgag gatgcttggc 60
acgtaccccc tgtacatact tcccgggcgc ccagcatgga aataaagcac ccagcgctgc 120
cctgggcccc tgcgagactg tgatggttct ttcca 155
<210> 186
<211> 860
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 186
agggagaagt gcccccacct gctcctcagt tccagcctga ccccctccca tcctttggcc 60
tctgaccctt tttccacagg ggacctaccc ctattgcggt cctccagctc atctttcacc 120
tcacccccct cctcctcctt ggctttaatt atgctaatgt tggaggagaa tgaataaata 180
aagtgaatct ttgcacctgt ggtttctctc tttcctcatt taataattat tatctgttgt 240
tttaccaact actcaatttc tcttataagg gactaaatat gtagtcatcc taaggcgcat 300
aaccatttat aaaaatcatc cttcattcta ttttacccta tcatcctctg caagacagtc 360
ctccctcaaa cccacaagcc ttctgtcctc acagtcccct gggccatggt aggagagact 420
tgcttccttg ttttcccctc ctcagcaagc cctcatagtc ctttttaagg gtgacaggtc 480
ttacagtcat atatcctttg attcaattcc ctgagaatca accaaagcaa atttttcaaa 540
agaagaaacc tgctataaag agaatcattc attgcaacat gatataaaat aacaacacaa 600
taaaagcaat taaataaaca aacaataggg aaatgtttaa gttcatcatg gtacttagac 660
ttaatggaat gtcatgcctt atttacattt ttaaacaggt actgagggac tcctgtctgc 720
caagggccgt attgagtact ttccacaacc taatttaatc cacactatac tgtgagatta 780
aaaacattca ttaaaatgtt gcaaaggttc tataaagctg agagacaaat atattctata 840
actcagcaat cccacttcta 860
<210> 187
<211> 983
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 187
gtgcgacggc cggcaagccc ccgctccccg ggctctcgcg gtcgcacgag gatgcttggc 60
acgtaccccc tgtacatact tcccgggcgc ccagcatgga aataaagcac ccagcgctgc 120
cctgggcccc tgcgagactg tgatggttct ttccacgggt caggccgagt ctgaggcctg 180
agtggcatga gggaggcaga gcgggtccca ctgtccccac actggcccag gctgtgcagg 240
tgtgcctggg ccgcctaggg tggggctcag ccaggggctg ccctcggcag ggtgggggat 300
ttgccagcgt ggccctccct ccagcagcac ctgccctggg ctgggccacg ggaagcccta 360
ggagcccctg gggacagaca cacagcccct gcctctgtag gagactgtcc tgttctgtga 420
gcgccctgtc ctccgacctc catgcccact cgggggcatg cctagtccat gtgcgtaggg 480
acaggccctc cctcacccat ctacccccac ggcactaacc cctggctgcc ctgcccagcc 540
tcgcacccgc atggggacac aaccgactcc ggggacatgc actctcgggc cctgtggagg 600
gactggtgca gatgcccaca cacacactca gcccagaccc gttcaacaaa ccccgcactg 660
aggttggccg gccacacggc caccacacac acacgtgcac gcctcacaca cggagcctca 720
cccgggcgaa ctgcacagca cccagaccag agcaaggtcc tcgcacacgt gaacactcct 780
cggacacagg cccccacgag ccccacgcgg cacctcaagg cccacgagcc tctcggcagc 840
ttctccacat gctgacctgc tcagacaaac ccagccctcc tctcacaagg gtgcccctgc 900
agccgccaca cacacacagg ggatcacaca ccacgtcacg tccctggccc tggcccactt 960
cccagtgccg cccttccctg cag 983
<210> 188
<211> 223
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 188
cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 60
aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 120
ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt 180
gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taa 223
<210> 189
<211> 222
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 189
cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 60
aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 120
ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 180
gggaggtttt ttaaagcaag taaaacctct acaaatgtgg ta 222
<210> 190
<211> 129
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 190
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 60
aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 120
tatcatgtc 129
<210> 191
<211> 249
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 191
gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 60
tgctttattt gtgaaatttg tgatgctatt gctttatttg tgaaatttgt gatgctattg 120
ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 180
ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca 240
aatgtggta 249
<210> 192
<211> 271
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 192
gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg 60
caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt 120
cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 180
cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 240
gccaacgtcg gggcggcagg ccctgccata g 271
<210> 193
<211> 271
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 193
gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg 60
caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt 120
cggtcccagg gctggcactc tgtcgatacc ccaccgagtc cccattgggg ccaatacgcc 180
cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 240
gccaacgtcg gggcggcagg ccctgccata g 271
<210> 194
<211> 194
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 194
gcggccgcgt cgaaattcac gcgtaagctt ctcgaccggg agatggggga ggctaactga 60
aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa 120
taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcgggat ctcgaggcta 180
gtctcgtgat cgat 194
<210> 195
<211> 223
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 195
taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 60
gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 120
caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 180
ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgg 223
<210> 196
<211> 279
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 196
gctggagcct cggtagccgt tcctcctgcc cgctgggcct cccaacgggc cctcctcccc 60
tccttgcacc ggcccttcct ggtctttgaa taaagtctga gtgggcggca gcctgtgtgt 120
gcctgggttc tctctgtccc ggaatgtgcc aacaatggag gtgtttacct gtctcagacc 180
aaggacctct ctgcagctgc atggggctgg ggagggagaa ctgcagggag tatgggaggg 240
gaagctgagg tgggcctgct caagagaagg tgctgaacc 279
<210> 197
<211> 255
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 197
ctgtcttctc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 60
ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 120
aaattgcatc aaatcgataa tatatggtag ggttcatagc cagagtaacc ttttttttta 180
atttttattt tattttattt ttgagtcggg cgcgccaaaa tgaagtgaag ttcctatact 240
ttctagagaa gacag 255
<210> 198
<211> 449
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 198
gatccttttc cctctgacca gaattatggg aacatcatga agccccttga gcatctagct 60
tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120
tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 180
tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 240
ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 300
acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 360
aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 420
atagctgtcc ctcttctctt atggagatc 449
<210> 199
<211> 449
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 199
gatccttttc cctctgacca gaattatggg aacatcatga agccccttga gcatctagct 60
tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120
tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 180
tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 240
ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 300
acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 360
aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 420
atagctgtcc ctcttctctt atggagatc 449
<210> 200
<211> 330
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 200
ctgtacttgg ctcactctcc ttctccttac ataggaaatt acccagttat gaaattaata 60
aaaagccagt gatccccaca tttgtctgtg cctctgccta ggggctggcc tgggagggga 120
gaaaaaggcc agaataattc caggaaccgc caagaaggca ggtcagagat cttgctggac 180
aaacagtggc tgaactctgt tccttaacag agtcagcagc agggggaggg gggggcggcg 240
cgcagtgtgg atcttatatc tagtccccag ggggaggggg caataaaaga tctttatttt 300
cattagatct gtgtgttggt tttttgtgtg 330
<210> 201
<211> 353
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 201
ttgacttgac tcatgcttgt ttcactttca catggaattt cccagttatg aaattaataa 60
aaatcaatgg tttccacatc tgtgtgtgcc tgtgtcaccg acccaggtag ggctggcctt 120
gggggagggg gaggccagaa tgactccaag agctacagga aggcaggtca gagatcccac 180
tggacaaaca gtggctggac tctgcaccat aacacacaat caacagggga gtgagctgga 240
tccaggggga ggggggggcg gcgcgcagtg tggatcttat atctagtccc cagggggagg 300
gggcaataaa agatctttat tttcattaga tctgtgtgtt ggttttttgt gtg 353
<210> 202
<211> 284
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 202
ttgacttgac tcatgcttgt ttcactttca catggaattt cccagttatg aaattaataa 60
aaatcaatgg tttccacatc tgtgtgtgcc tgtgtcaccg acccaggtag ggctggcctt 120
gggggagggg gaggccagaa tgactccaag agctacagga aggcaggtca gagaaggggg 180
aggggggggc ggcgcgcagt gtggatctta tatctagtcc ccagggggag ggggcaataa 240
aagatcttta ttttcattag atctgtgtgt tggttttttg tgtg 284
<210> 203
<211> 280
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 203
tgtatactct atattatact ctatgttata ctctgtaatc ctactcaata aacgtgtcac 60
gcctgtgaaa ccgtactaag tctcccgtgt cttcttatca ccatcaggtg acatcctcgc 120
ccaggctgtc aatcatgccg gtatcgattc cagtagcacc ggccccacgc tgacaaccca 180
ctcttgcagc gttagcagcg cccctcttaa caagccgacc cccaccagcg tcgcggttac 240
taacactcct ctccccgggg catccgctac tcccgagctc 280
<210> 204
<211> 347
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 204
ctgtcttcat ccacaagccc agctccccac ccatcaccat ggacaatgtt tttttactaa 60
cacttggaca atgatggata cttttttact aacacttgga caatgatgat gatacactcc 120
tcacttgccc acttagacac aattactaac accacacccc ctcttttatt tctctgtact 180
taatgttttc tgaataaagt gatcctattg tacccacatt aaagacttct ttaactcttt 240
atggttcaca ggacccgaga tgaacataga tattgttaca gcagcggcct ccatgtcagg 300
tataactact gcctcacaca gcgccctgcc aatcagaagg aagacag 347
<210> 205
<211> 332
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 205
ctgtcttccc acagacctgg tgaccgtcag gaagaagatt cagtgagagg acacgaggta 60
tgtcatggtt tttaatcaat aaataaagag gttttattca tcggacagtc gttgtagcct 120
gtaaaagact cgccccggag ggggttcccc cgatgtgagg ggcatgcagt agtatggtgt 180
cctgagtgtc tcggatgcgt ccttgaactc gcactctacc gccgtggggg ttaataaagt 240
tttgctgcgc cggtaggggg ggaggccgag gataataaag ttgctacgta ctggttgaag 300
tctaacaatc tctcgggggg atccgaagac ag 332
<210> 206
<211> 138
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 206
ctgtcttctc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 60
ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 120
aaattgcatc gaagacag 138
<210> 207
<211> 403
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 207
ctgtcttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 60
tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 120
ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 180
taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 240
gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 300
tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 360
ccagtcatag ctgtccctct tctcttatgg agatcgaaga cag 403
<210> 208
<211> 287
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 208
ctgtcttcgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 60
tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac 120
gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagtccc cattggggcc 180
aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 240
ggctcgcagc caacgtcggg gcggcaggcc ctgccatagg aagacag 287
<210> 209
<211> 462
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 209
tcttctttct agactgacca aagacttttt gtcaacttgt acaatctgaa gcaatgtctg 60
gcccacagac agctgagctg taaacaaatg tcacatggaa ataaatactt tatcttgtga 120
actcacttta ttgtgaagga atttgttttg tttttcaaac ctttcctgcg gtgttgacag 180
cccaaggatt atctgaatag agcctaggaa ctggaaatgg aacagtgcag tctgatggta 240
cttaagggag aaagagggaa aggaggtgtg gaagaagaaa aaagagaagc aagggggggg 300
ggagaaaggg agagggagag ggagagggag agggagaggg agagggagag ggagagggag 360
agggagaggg agagggagag ggagggggag agagagagag agagagagag agagagagag 420
agagagagag agagagagag agagcatgca ctctaacagc aa 462
<210> 210
<211> 463
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 210
tcttctttct agactgacca aagacttttt gtcaacttgt acaatctgaa gcaatgtctg 60
gcccacagac agctgagctg taaacaaatg tcacatggaa ataaatactt tatcttgtga 120
actcacttta ttgtgaagga atttgttttg tttttcaaac ctttcctgcg gtgttgacag 180
cccaaggatt atctgaatag agcctaggaa ctggaaatgg aacagtgcag tctgatggta 240
cttaagggag aaagagggaa aggaggtgtg gaagaagaaa aaagagaagc aagggggagg 300
gggagaaagg gagagggaga gggagaggga gagggagagg gagagggaga gggagaggga 360
gagggagagg gagagggagg gggaggggga gagagagaga gagagagaga gagagagaga 420
gagagagaga gagagagaga gagagcatgc actctaacag caa 463
<210> 211
<211> 415
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 211
tcttctttct agactgacca aagacttttt gtcaacttgt acaatctgaa gcaatgtctg 60
gcccacagac agctgagctg taaacaaatg tcacatggaa ataaatactt tatcttgtga 120
actcacttta ttgtgaagga atttgttttg tttttcaaac ctttcctgcg gtgttgacag 180
cccaaggatt atctgaatag agcctaggaa ctggaaatgg aacagtgcag tctgatggta 240
cttaagggag aaagagggaa aggaggtgtg gaagaagaaa aaagagaagc aagggggagg 300
gggagaaagg gagagggaga gggagaggga gagggagagg gagagggaga gggagaggga 360
gagggagagg gagagggagg gggaggggga gagagagcat gcactctaac agcaa 415
<210> 212
<211> 463
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 212
tcttctttct agactgacca aagacttttt gtcaacttgt acaatctgaa gcaatgtctg 60
gcccacagac agctgagctg taaacaaatg tcacatggaa ataaatactt tatcttgtga 120
actcacttta ttgtgaagga atttgttttg tttttcaaac ctttcctgcg gtgttgacag 180
cccaaggatt atctgaatag agcctaggaa ctggaaatgg aacagtgcag tctgatggta 240
cttaagggag aaagagggaa aggaggtgtg gaagaagaaa aaagagaagc acgggggagg 300
gggagaaagg gagagggaga gggagaggga gagggagagg gagagggaga gggagaggga 360
gagggagagg gagagggagg gggaggggga gagagagaga gagagagaga gagagagaga 420
gagagagaga gagagagaga gagagcatgc actctaacag caa 463
<210> 213
<211> 461
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 213
tcttctttct agactgacca aagacttttt gtcaacttgt acaatctgaa gcaatgtctg 60
gcccacagac agctgagctg taaacaaatg tcacatggaa ataaatactt tatcttgtga 120
actcacttta ttgtgaagga atttgttttg tttttcaaac ctttcctgcg gtgttgacag 180
cccaaggatt atctgaatag agcctaggaa ctggaaatgg aacagtgcag tctgatggta 240
cttaagggag aaagagggaa aggaggtgtg gaagaggaaa gaagagaagc aagggggagg 300
gggagaaagg gagagggaga gggagaggga gagggagagg gagagggaga gggagaggga 360
gagggagagg gagagggagg gggaggggga gagagagaga gagagaggga gagagagaga 420
gagagagaga gagagagaga gagcatgcac tctaacagca a 461
<210> 214
<211> 147
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 214
ctgtcttccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 60
aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 120
caatgtatct tatcatgtcg aagacag 147
<210> 215
<211> 239
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 215
ctgtcttcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 60
gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 120
aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 180
ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta agaagacag 239
<210> 216
<211> 231
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 216
cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 60
aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 120
ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt 180
gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taagaagaca g 231
<210> 217
<211> 65
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 217
ctgtcttcaa taaaagatct ttattttcat tagatctgtg tgttggtttt ttgtgtggaa 60
gacag 65
<210> 218
<211> 2836
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 218
ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc 60
ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 120
tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 180
ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 240
tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc 300
tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 360
actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 420
cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 480
gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 540
tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 600
ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 660
ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 720
cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 780
cctttgagtg agctgatacc gctcaaggct gagcggccgc ttaacccttt cactgccagc 840
cgatttgtcc caaagtggaa cttgtattgc cagacagttt ttgaacgttt tgcactgttt 900
cactttaggg gcctttcctc gaggggactt ttagtttacc caggaaaaca atatattgtt 960
tttttcagaa caacctaagc tttcaaaata tggtagaatt tttgtgtaat tccaattctg 1020
taacaagata taggcttcta aatgtctaaa aatgcaaacc taggtggagt gtggaactga 1080
acctccgtgg gagtcttgag tgtgccaggc cctctctccg tgaaggaggc aatgcctgtg 1140
ggcgtcgccg ttgccgggac ggtctggcac acgcaggcgt gtggctctcg ttcatttcca 1200
cgtagaagtc cagagcgaca ccccagagag gagatgcctc cccggcgtga tggcctgacg 1260
atggattccc gcgtgcggca acgtggggag tctgcagtgt ggccggtttg gaacctggca 1320
aggagagcga aggcaccgtg ccgggcttgc acccttccct gcatgtttcc gggtgcccgc 1380
agagctccgg gagcaaacag tcggcatggc cagcctttcg ggggccggag agacttgagc 1440
aacaggccgc cttgcggagg gcaaagccac gcggaaacca aaatcacgcc tccgtcgtcc 1500
tgcgtgtggc tcctccgtgg ccggggctgt cggcctcgcg ccgcgttgca gggctcagcc 1560
tggggatgtg cggtctgtga accgcgcggg tgaacacccg acggcaaccc gagtcccggt 1620
cttttgtccc ggaggaaacc gcccactccc tgggccacgg aaccggggcg aatgggtggt 1680
gccccgccgg ccggcgcggc ggctgtgggc ccagccctca gcccgcgccg gacgctgacc 1740
gttttcccgg agggcggggg tcccgctact cccggaggcc gaggaccgct tttcctccct 1800
gccttcctcc ccccgttccc cggctccctc ccgcccgccc ccagtccctg cgtcgctctg 1860
tctttccctc cgttcctccc tgcctccctg cctccctccc tccctcctaa cgtccctccg 1920
cccgtccttc cgcccctcta ggactcccgt tcctctctcc atctctgccc gccttccctc 1980
ccgcctggaa cgctcagcgt ccccggtgtg cgccgggcct ggggtctgcg ttccgccgcc 2040
aggcgctccg tgctggcacc tgggcggctg caggggcccg ggcgggcggg cgacggtggc 2100
gcgggggcgc ataggaggcg agccgccgga gcggtgtcag gcccggacgc tgcgcggggc 2160
ccggtgtttc gcgggacggg ggtcaccacc cagcccaggg gacgacgcgt tttccggggg 2220
tggggggtgg ggggtgggga gggggcggtc aggcggcggg gtgggctggt ggagaggcag 2280
gagagctctg cccgggctgc tcccacagcc caggcggctg cccgcaaacc cgcgcgtgcg 2340
cagtaggcgg cccacctggt gttcagcgaa gggcgacaca aaattttctg atctccataa 2400
gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa 2460
aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa 2520
aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg 2580
tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc 2640
atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc 2700
atatgttctt ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa 2760
tttcctttat tagccattaa ttaactctgg agacggcaca tcgcccacag tccccgagaa 2820
gttgggaggg gtcggc 2836
<210> 219
<211> 5861
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 219
cgtctcaggg gatgcccccg ccccgcctgc tgttcttcct cctcttcctg accccaatgg 60
aagtccgccc tgaggaaccg ctcgtcgtga aagtggaaga aggagacaac gccgtgcttc 120
agtgcctcaa gggcacctcc gacgggccga cccagcaact cacttggagc agagagagcc 180
cgctcaagcc cttcctgaaa ctgagcttgg ggcttccggg gctgggtatt catatgcggc 240
ccctcgcaat ctggttgttt atctttaacg tgtcacagca gatgggtggc ttttacctgt 300
gccagcccgg gcctccttcg gaaaaggcct ggcagcctgg atggactgtg aatgtcgaag 360
gctcgggaga gctgttcaga tggaacgtgt ccgacctggg aggcttgggc tgcggtctga 420
agaacagatc ctccgagggg ccgagctccc cctccggaaa gctgatgtca cctaagctct 480
acgtgtgggc taaggacagg cccgagattt gggaagggga gccgccttgt ttgcctcctc 540
gggactcact caaccagtcg ctgagccagg atctcactat ggcccccggg tccacgctgt 600
ggctgtcctg cggagtcccc ccggactcag tgtcgagagg acccctgtcg tggacgcacg 660
tgcatccgaa gggccctaag agcctgctga gccttgagct gaaggacgac cgccccgcaa 720
gggatatgtg ggtcatggaa accggactgc tcctgccgcg ggcgaccgcc caagatgccg 780
gaaagtatta ctgccaccgc ggcaacctga ctatgagctt ccacctggaa attaccgcgc 840
ggccagtgct gtggcactgg ctgctgcgga ctggcggatg gaaagtgtcc gccgtgaccc 900
tggcttacct gatcttctgc ctgtgttccc tcgtgggaat tctccatctg caacgcgctc 960
tcgtgctgcg gcgcaagcgc aagcggatga ccgacccaac tagaaggttc ttcaaggtca 1020
ccccgccgcc cggctcgggg ccacagaatc agtacggcaa cgtgctgtca ctgccgaccc 1080
ccacttccgg cctgggacgg gcacaacgct gggccgcggg cctcggtggc accgccccgt 1140
cctacggcaa cccgtcctcc gacgtgcaag ccgatggtgc cctggggtcc cgctcccctc 1200
cgggagtggg acccgaagaa gaagagggag agggttacga agaacccgac tcagaagagg 1260
actccgaatt ctacgaaaac gatagcaacc tgggacagga tcagctgtcc caagacggat 1320
cgggctacga gaaccccgag gacgaacccc tgggaccaga ggatgaggac tccttctcca 1380
atgctgagtc gtacgagaac gaggacgaag aactgacaca gccagtggcc aggaccatgg 1440
acttcctttc ccctcacggt tcggcgtggg acccgtcccg cgaggccacc tccctgggaa 1500
gccagtcata cgaagatatg cggggcatcc tctacgcggc gccgcagctt agatcgatcc 1560
gaggacagcc tggacccaac cacgaagagg acgccgactc ctatgaaaac atggacaacc 1620
ctgatggacc tgaccctgcc tgggggggtg gcggccggat gggcacctgg tctacccggt 1680
aatgaggtta attgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 1740
gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 1800
agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 1860
gaatacaata gcaggcatgc tggggatgcg gtgggctcta tgggtaccca ggtgctgaag 1920
aattgacccg gttcctcctg ggttataatg gttacaaata aagcaatagc atcacaaatt 1980
tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 2040
tatcttatca tgtctggaag acaccacagg taccaatatt ctcactgact ccgtcctgga 2100
gttggatgag agataatggc cttacgttgt gccaggggag ggtcgggctg gatttagcaa 2160
gatttacctt ctccaaagag cggtgctgca gtggcacagc tgcccacgga ggtggggggg 2220
tcaccgtccc tggaggtgat gaagaactgt ggggatgtgg cactgaggga catggccagt 2280
gggcacggtg ggtgggttgg ggttggtctt ggggatcttg gagggctttt ccagccttca 2340
tgatttgacg attgtatgaa catctacatg gcaattctcc agctgcctgt cccagtccta 2400
ctgacccagc tgtatctctc caggcaagca cttccacccc ttctgcttgc atccagacac 2460
catcaaacat gcaggctcag acacagggac cagcagtgtc tgtggccttt ttgtgctcct 2520
ctccatgctg ggttttaact tgctctttgt ccttctatcc tatcttctta tccttaaggc 2580
tgttctgaac gctgtgactt ggagagtgtc ccagagccct caacaccagc atgtcccacg 2640
tccatgctgt cctgcacttc cttatcccca agatctgcct ctccgtgatg cactgaattg 2700
gcaaacatgt gtcaccccag accaacaatg tcacagcaaa ctcccccttg ataggacaag 2760
ggggaatggc tttacactga gacaggggag gtttgggttg gatatgagga ggcagttttt 2820
cccccagagg gtggtgacgc actgaacagg ttgcccaagg aggctgtgga tgccccatcc 2880
ctgcaggcat tcaaggccag gctggatgtg gctctgggca gcctgggctg ctggttgatg 2940
accctgcaca tagcaggggg ttggatctgg atgagcactg tgctcctttg caacccaggc 3000
cgttctatga ttctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc 3060
ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg 3120
ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc 3180
gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta 3240
attacatccc tgggggcttt gggggggggc tgtccccgtg agctcgcgct agcgacagcc 3300
cccccggctc gttgcccaga ggggctgtca tcgctagaaa ctctctgcag cggcggcaca 3360
tgctgccgct gcagagagaa gaattaaaat gccaacaacg tatgggacgc gtcgttggca 3420
tttaagccct tttactgcca cgacgtatcc catgcgtgat tggcaggcaa agggttaagg 3480
cgcgccgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg 3540
gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaagagag caggtttact 3600
gataggtatc gagatcgacg gccttgacca cttccaccag gcacatgtga tctctcctct 3660
catcgcggtc tttggagagc ttagtgtgat aagtgatatg atggtagcgc ggaatgtgga 3720
cagccgctga accggccagt ggccgattca tctggctaca cttggtcacc agcttctccg 3780
tcaccccctc gatgtcgtag gcttgattga actccacgcg gattccgttg ttcacagtgt 3840
cggggagaat gtaaaggatg ctgggagggc actggaaggc gacattcttc cgaagaatat 3900
gcccgtcctt cttaaagttt tctccagtca gagtcacccg gttgtagata gatcccctct 3960
cataggtgac catcgcgcgg gtcttgtaca ctccatctcc ctcaaaagaa atggtgcgct 4020
cttgggtata accttccggc atggcggatt tgaagaagtc cttaatgtgg ctagggtact 4080
tagcaaaaca ctgcactcca tacgagaggg ttgacacaag ggtggcccaa ggcactggca 4140
gatctccagt ggtacagata tacttggcct taatggttcc agtcgtagcg tccccggttc 4200
cttctccctt gatgatgaac ttcattcctt cgacgtcccc ttccagctcg gtgatgtacg 4260
gaatctcctt ctcaaacagc ttagcacctt cggtcagggc agtcatggtg gcggcgtcac 4320
tcttggcacg gggaatccgc gttccaatgc accgttcccg gccgcggagg ctggatcggt 4380
cccggtgtct tctatggagg tcaaaacagc gtggatgggc tctccaggcg atctgacggt 4440
tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg 4500
tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca 4560
aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc 4620
cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt 4680
agatgtactg ccaagtagga aagtcccgta aggtcatgta ctgggcataa tgccaggcgg 4740
gccatttacc gtcattgacg tcaatagggg gcggacttgg catatgatac acttgatgta 4800
ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta 4860
ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg 4920
gtcagccagg cgggccattt accgtaagtc gtctacagag ccccgtacgc cagagccccc 4980
gcagtgtcga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga 5040
ggaagtaaaa aatgagccat atccaacggg aaacgtcgag gccgcgatta aattccaaca 5100
tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga 5160
caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag 5220
gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta 5280
tgccacttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca 5340
ctgcgatccc cggaaaaaca gcgttccagg tattagaaga atatcctgat tcaggtgaaa 5400
atattgttga tgcgctggca gtgttcctgc gccggttgca ctcgattcct gtttgtaatt 5460
gtccttttaa cagcgatcgc gtatttcgcc tcgctcaggc gcaatcacga atgaataacg 5520
gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct 5580
ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt 5640
tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac 5700
gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt 5760
tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga 5820
ataaattgca gtttcatttg atgctcgatg agtttttcta a 5861
<210> 220
<211> 2795
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 220
ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc 60
ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 120
tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 180
ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 240
tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc 300
tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 360
actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 420
cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 480
gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 540
tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 600
ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 660
ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 720
cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 780
cctttgagtg agctgatacc gctcaaggct gagcggccgc ttaacccttt cactgccagc 840
cgatttgtcc caaagtggaa cttgtattgc cagacagttt ttgaacgttt tgcactgttt 900
cactttaggg gcctttcctc gaggggactt ttagtttacc caggaaaaca atatattgtt 960
tttttcagaa caacctaagc tttcaaaata tggtagaatt tttgtgtaat tccaattctg 1020
taacaagata taggcttcta aatgtctaaa aatgcaaacc taggtggagt gtggaactga 1080
acctccgtgg gagtcttgag tgtgccaggc cctctctccg tgaaggaggc aatgcctgtg 1140
ggcgtcgccg ttgccgggac ggtctggcac acgcaggcgt gtggctctcg ttcatttcca 1200
cgtagaagtc cagagcgaca ccccagagag gagatgcctc cccggcgtga tggcctgacg 1260
atggattccc gcgtgcggca acgtggggag tctgcagtgt ggccggtttg gaacctggca 1320
aggagagcga aggcaccgtg ccgggcttgc acccttccct gcatgtttcc gggtgcccgc 1380
agagctccgg gagcaaacag tcggcatggc cagcctttcg ggggccggag agacttgagc 1440
aacaggccgc cttgcggagg gcaaagccac gcggaaacca aaatcacgcc tccgtcgtcc 1500
tgcgtgtggc tcctccgtgg ccggggctgt cggcctcgcg ccgcgttgca gggctcagcc 1560
tggggatgtg cggtctgtga accgcgcggg tgaacacccg acggcaaccc gagtcccggt 1620
cttttgtccc ggaggaaacc gcccactccc tgggccacgg aaccggggcg aatgggtggt 1680
gccccgccgg ccggcgcggc ggctgtgggc ccagccctca gcccgcgccg gacgctgacc 1740
gttttcccgg agggcggggg tcccgctact cccggaggcc gaggaccgct tttcctccct 1800
gccttcctcc ccccgttccc cggctccctc ccgcccgccc ccagtccctg cgtcgctctg 1860
tctttccctc cgttcctccc tgcctccctg cctccctccc tccctcctaa cgtccctccg 1920
cccgtccttc cgcccctcta ggactcccgt tcctctctcc atctctgccc gccttccctc 1980
ccgcctggaa cgctcagcgt ccccggtgtg cgccgggcct ggggtctgcg ttccgccgcc 2040
aggcgctccg tgctggcacc tgggcggctg caggggcccg ggcgggcggg cgacggtggc 2100
gcgggggcgc ataggaggcg agccgccgga gcggtgtcag gcccggacgc tgcgcggggc 2160
ccggtgtttc gcgggacggg ggtcaccacc cagcccaggg gacgacgcgt tttccggggg 2220
tggggggtgg ggggtgggga gggggcggtc aggcggcggg gtgggctggt ggagaggcag 2280
gagagctctg cccgggctgc tcccacagcc caggcggctg cccgcaaacc cgcgcgtgcg 2340
cagtaggcgg cccacctggt gttcagcgaa gggcgacaca aaattttctg atctccataa 2400
gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa 2460
aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatataaa 2520
aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag cagggggctg 2580
tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca gcatatgggc 2640
atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat ttgccctccc 2700
atatgttctt ccgagtgaga gacacaaaaa attccaacac actattgcaa tgaaaataaa 2760
tttcctttat tagccattaa ttaactctgg agacg 2795
<210> 221
<211> 5907
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 221
agcttgcttg ttctttttgc agaagctcag aataaacgct caactttggc cgccaccatg 60
cccccgcccc gcctgctgtt cttcctcctc ttcctgaccc caatggaagt ccgccctgag 120
gaaccgctcg tcgtgaaagt ggaagaagga gacaacgccg tgcttcagtg cctcaagggc 180
acctccgacg ggccgaccca gcaactcact tggagcagag agagcccgct caagcccttc 240
ctgaaactga gcttggggct tccggggctg ggtattcata tgcggcccct cgcaatctgg 300
ttgtttatct ttaacgtgtc acagcagatg ggtggctttt acctgtgcca gcccgggcct 360
ccttcggaaa aggcctggca gcctggatgg actgtgaatg tcgaaggctc gggagagctg 420
ttcagatgga acgtgtccga cctgggaggc ttgggctgcg gtctgaagaa cagatcctcc 480
gaggggccga gctccccctc cggaaagctg atgtcaccta agctctacgt gtgggctaag 540
gacaggcccg agatttggga aggggagccg ccttgtttgc ctcctcggga ctcactcaac 600
cagtcgctga gccaggatct cactatggcc cccgggtcca cgctgtggct gtcctgcgga 660
gtccccccgg actcagtgtc gagaggaccc ctgtcgtgga cgcacgtgca tccgaagggc 720
cctaagagcc tgctgagcct tgagctgaag gacgaccgcc ccgcaaggga tatgtgggtc 780
atggaaaccg gactgctcct gccgcgggcg accgcccaag atgccggaaa gtattactgc 840
caccgcggca acctgactat gagcttccac ctggaaatta ccgcgcggcc agtgctgtgg 900
cactggctgc tgcggactgg cggatggaaa gtgtccgccg tgaccctggc ttacctgatc 960
ttctgcctgt gttccctcgt gggaattctc catctgcaac gcgctctcgt gctgcggcgc 1020
aagcgcaagc ggatgaccga cccaactaga aggttcttca aggtcacccc gccgcccggc 1080
tcggggccac agaatcagta cggcaacgtg ctgtcactgc cgacccccac ttccggcctg 1140
ggacgggcac aacgctgggc cgcgggcctc ggtggcaccg ccccgtccta cggcaacccg 1200
tcctccgacg tgcaagccga tggtgccctg gggtcccgct cccctccggg agtgggaccc 1260
gaagaagaag agggagaggg ttacgaagaa cccgactcag aagaggactc cgaattctac 1320
gaaaacgata gcaacctggg acaggatcag ctgtcccaag acggatcggg ctacgagaac 1380
cccgaggacg aacccctggg accagaggat gaggactcct tctccaatgc tgagtcgtac 1440
gagaacgagg acgaagaact gacacagcca gtggccagga ccatggactt cctttcccct 1500
cacggttcgg cgtgggaccc gtcccgcgag gccacctccc tgggaagcca gtcatacgaa 1560
gatatgcggg gcatcctcta cgcggcgccg cagcttagat cgatccgagg acagcctgga 1620
cccaaccacg aagaggacgc cgactcctat gaaaacatgg acaaccctga tggacctgac 1680
cctgcctggg ggggtggcgg ccggatgggc acctggtcta cccggtaatg aggttaattg 1740
ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 1800
cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 1860
tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaat acaatagcag 1920
gcatgctggg gatgcggtgg gctctatggg tacccaggtg ctgaagaatt gacccggttc 1980
ctcctgggtt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 2040
tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 2100
tggaagacac cacaggtacc aatattctca ctgactccgt cctggagttg gatgagagat 2160
aatggcctta cgttgtgcca ggggagggtc gggctggatt tagcaagatt taccttctcc 2220
aaagagcggt gctgcagtgg cacagctgcc cacggaggtg ggggggtcac cgtccctgga 2280
ggtgatgaag aactgtgggg atgtggcact gagggacatg gccagtgggc acggtgggtg 2340
ggttggggtt ggtcttgggg atcttggagg gcttttccag ccttcatgat ttgacgattg 2400
tatgaacatc tacatggcaa ttctccagct gcctgtccca gtcctactga cccagctgta 2460
tctctccagg caagcacttc caccccttct gcttgcatcc agacaccatc aaacatgcag 2520
gctcagacac agggaccagc agtgtctgtg gcctttttgt gctcctctcc atgctgggtt 2580
ttaacttgct ctttgtcctt ctatcctatc ttcttatcct taaggctgtt ctgaacgctg 2640
tgacttggag agtgtcccag agccctcaac accagcatgt cccacgtcca tgctgtcctg 2700
cacttcctta tccccaagat ctgcctctcc gtgatgcact gaattggcaa acatgtgtca 2760
ccccagacca acaatgtcac agcaaactcc cccttgatag gacaaggggg aatggcttta 2820
cactgagaca ggggaggttt gggttggata tgaggaggca gtttttcccc cagagggtgg 2880
tgacgcactg aacaggttgc ccaaggaggc tgtggatgcc ccatccctgc aggcattcaa 2940
ggccaggctg gatgtggctc tgggcagcct gggctgctgg ttgatgaccc tgcacatagc 3000
agggggttgg atctggatga gcactgtgct cctttgcaac ccaggccgtt ctatgattct 3060
gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc 3120
aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg 3180
cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg 3240
gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg 3300
ggctttgggg gggggctgtc cccgtgagct cgcgctagcg acagcccccc cggctcgttg 3360
cccagagggg ctgtcatcgc tagaaactct ctgcagcggc ggcacatgct gccgctgcag 3420
agagaagaat taaaatgcca acaacgtatg ggacgcgtcg ttggcattta agccctttta 3480
ctgccacgac gtatcccatg cgtgattggc aggcaaaggg ttaaggcgcg ccgatgcaat 3540
ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac 3600
gggggagggg caaacaacag atggctggca agagagcagg tttactgata ggtatcgaga 3660
tcgacggcct tgaccacttc caccaggcac atgtgatctc tcctctcatc gcggtctttg 3720
gagagcttag tgtgataagt gatatgatgg tagcgcggaa tgtggacagc cgctgaaccg 3780
gccagtggcc gattcatctg gctacacttg gtcaccagct tctccgtcac cccctcgatg 3840
tcgtaggctt gattgaactc cacgcggatt ccgttgttca cagtgtcggg gagaatgtaa 3900
aggatgctgg gagggcactg gaaggcgaca ttcttccgaa gaatatgccc gtccttctta 3960
aagttttctc cagtcagagt cacccggttg tagatagatc ccctctcata ggtgaccatc 4020
gcgcgggtct tgtacactcc atctccctca aaagaaatgg tgcgctcttg ggtataacct 4080
tccggcatgg cggatttgaa gaagtcctta atgtggctag ggtacttagc aaaacactgc 4140
actccatacg agagggttga cacaagggtg gcccaaggca ctggcagatc tccagtggta 4200
cagatatact tggccttaat ggttccagtc gtagcgtccc cggttccttc tcccttgatg 4260
atgaacttca ttccttcgac gtccccttcc agctcggtga tgtacggaat ctccttctca 4320
aacagcttag caccttcggt cagggcagtc atggtggcgg cgtcactctt ggcacgggga 4380
atccgcgttc caatgcaccg ttcccggccg cggaggctgg atcggtcccg gtgtcttcta 4440
tggaggtcaa aacagcgtgg atgggctctc caggcgatct gacggttcac taaacgagct 4500
ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag 4560
ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg 4620
tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg 4680
tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa 4740
gtaggaaagt cccgtaaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca 4800
ttgacgtcaa tagggggcgg acttggcata tgatacactt gatgtactgc caagtgggca 4860
gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg 4920
ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg 4980
ccatttaccg taagtcgtct acagagcccc gtacgccaga gcccccgcag tgtcgacaat 5040
taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa gtaaaaaatg 5100
agccatatcc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta 5160
tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg 5220
tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 5280
gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc acttccgacc 5340
atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga 5400
aaaacagcgt tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 5460
ctggcagtgt tcctgcgccg gttgcactcg attcctgttt gtaattgtcc ttttaacagc 5520
gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 5580
agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 5640
aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 5700
cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 5760
gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 5820
cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 5880
catttgatgc tcgatgagtt tttctaa 5907
<210> 222
<211> 1845
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 222
cgaagaacaa cgagaagatc ctcaacttct cctaagcctt ttcactaata gggagaagtt 60
cgatggggca gccttgggca gacccacact tctgctccat ttccctggtt cctgcagctc 120
tcagattctc ccattttatt cgggaagcag ctttctggtt tctgggtcct ggatgtcctt 180
ggtgcacact ccaaggactc ctcgtcctta atccatagtc tgtattccct gagtcctatc 240
ctgggaaccc tcatccggtc acttcctcgg cgggacaatc tcagctcccc tccccctctc 300
aggtcggagc ccacacgctt ggtgcgtgca catttcaaaa acgaggcggg tccaaaaaga 360
gggagggggg gaatgagaga ggcccagcta ctcgcggctt tacgggtgca cgtagctcag 420
gcctctgcgc ccttgagctg ggactggatg agccgagcgg gaggcggggc gcgcgtcatc 480
agctcccccc accatccagt tcctataaat acggactgca gccctccctg gtgctctctg 540
ctcctccctg ttctagagac agccgcatct tcttgtgcag tgccaggtga aaaagaaaag 600
aaaaaaaagg actgggccgc aggaggccgg agaggaatgg aaattaggaa tggggggaag 660
gacgctgtac gggtttaggg gcgctggtgc gaggtccgga agccgagccc aggctccgca 720
ttgcagagga tggtagagga cgtgatgggg catgcggcgg gaatggaggc gggtgggggg 780
aggggactgg ccacgctaat ctgactttct tctcccgcag cctcttctca tagacaagag 840
cttgcttgtt ctttttgcag aagggccgcc accatgactg ccctgaccga aggtgctaag 900
ctgtttgaga aggagattcc gtacatcacc gagctggaag gggacgtcga aggaatgaag 960
ttcatcatca agggagaagg aaccggggac gctacgactg gaaccattaa ggccaagtat 1020
atctgtacca ctggagatct gccagtgcct tgggccaccc ttgtgtcaac cctctcgtat 1080
ggagtgcagt gttttgctaa gtaccctagc cacattaagg acttcttcaa atccgccatg 1140
ccggaaggtt atacccaaga gcgcaccatt tcttttgagg gagatggagt gtacaagacc 1200
cgcgcgatgg tcacctatga gaggggatct atctacaacc gggtgactct gactggagaa 1260
aactttaaga aggacgggca tattcttcgg aagaatgtcg ccttccagtg ccctcccagc 1320
atcctttaca ttctccccga cactgtgaac aacggaatcc gcgtggagtt caatcaagcc 1380
tacgacatcg agggggtgac ggagaagctg gtgaccaagt gtagccagat gaatcggcca 1440
ctggccggtt cagcggctgt ccacattccg cgctaccatc atatcactta tcacactaag 1500
ctctccaaag accgcgatga gaggagagat cacatgtgcc tggtggaagt ggtcaaggcc 1560
gtcgatctcg atacctatca gtaaacctgc tctcttgcca gccatctgtt gtttgcccct 1620
cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 1680
aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 1740
aggacagcaa gggggaggat tgggaataca atagcaggca tgctggggat gcggtgggct 1800
ctatgggtac ccaggtgctg aagaattgac ccggttcctc ctggg 1845
<210> 223
<211> 10148
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 223
cctctcccgg ccagaggagc aaggtatgcg ggaggcgacc aggaggatag cggggctgac 60
gtcgggaggt ggcctccgtg ggaaggacac ccggatcttg acacagcctt ggcagcggag 120
tcaggaagag taggggtagg ttctggacgc cctcttggcc agctcatcgc cgccccaccc 180
tctgctggag cacagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc 240
cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg 300
aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg 360
gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga 420
aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag 480
tgcggtagtc gccttggacg ttctttttcg caacgggttt gccgtcagaa cgcaggtgag 540
tggcgggtgt ggcttccgcg ggccccggag ctggagccct gctctgagcg ggccgggctg 600
atatgcgagt gtcgtccgca gggtttagct gtgagcattc ccacttcgag tggcgggcgg 660
tgcgggggtg agagtgcgag gcctagcggc aaccccgtag cctcgcctcg tgtccggctt 720
gaggcctagc gtggtgtccg ccgccgcgtg ccactccggc cgcactatgc gttttttgtc 780
cttgctgccc tcgattgcct tccagcagca tgggctaaca aagggagggt gtggggctca 840
ctcttaagga gcccatgaag cttacgttgg ataggaatgg aagggcagga ggggcgactg 900
gggcccgccc gccttcggag cacatgtccg acgccacctg gatggggcga ggcctgtggc 960
tttccgaagc aatcgggcgt gagtttagcc tacctgggcc atgtggccct agcactgggc 1020
acggtctggc ctggcggtgc cgcgttccct tgcctcccaa caagggtgag gccgtcccgc 1080
ccggcaccag ttgcttgcgc ggaaagatgg ccgctcccgg ggccctgttg caaggagctc 1140
aaaatggagg acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga 1200
gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg tggtctatcg gccgcatagt 1260
cacctcgggc ttctcttgag caccgctcgt cgcggcgggg ggaggggatc taatggcgtt 1320
ggagtttgtt cacatttggt gggtggagac tagtcaggcc agcctggcgc tggaagtcat 1380
tcttggaatt tgcccctttg agtttggagc gaggctaatt ctcaagcctc ttagcggttc 1440
aaaggtattt tctaaacccg tttccaggtg ttgtgaaagc caccgctaat tcaaagcaag 1500
ccgccaccat gcccccgccc cgcctgctgt tcttcctcct cttcctgacc ccaatggaag 1560
tccgccctga ggaaccgctc gtcgtgaaag tggaagaagg agacaacgcc gtgcttcagt 1620
gcctcaaggg cacctccgac gggccgaccc agcaactcac ttggagcaga gagagcccgc 1680
tcaagccctt cctgaaactg agcttggggc ttccggggct gggtattcat atgcggcccc 1740
tcgcaatctg gttgtttatc tttaacgtgt cacagcagat gggtggcttt tacctgtgcc 1800
agcccgggcc tccttcggaa aaggcctggc agcctggatg gactgtgaat gtcgaaggct 1860
cgggagagct gttcagatgg aacgtgtccg acctgggagg cttgggctgc ggtctgaaga 1920
acagatcctc cgaggggccg agctccccct ccggaaagct gatgtcacct aagctctacg 1980
tgtgggctaa ggacaggccc gagatttggg aaggggagcc gccttgtttg cctcctcggg 2040
actcactcaa ccagtcgctg agccaggatc tcactatggc ccccgggtcc acgctgtggc 2100
tgtcctgcgg agtccccccg gactcagtgt cgagaggacc cctgtcgtgg acgcacgtgc 2160
atccgaaggg ccctaagagc ctgctgagcc ttgagctgaa ggacgaccgc cccgcaaggg 2220
atatgtgggt catggaaacc ggactgctcc tgccgcgggc gaccgcccaa gatgccggaa 2280
agtattactg ccaccgcggc aacctgacta tgagcttcca cctggaaatt accgcgcggc 2340
cagtgctgtg gcactggctg ctgcggactg gcggatggaa agtgtccgcc gtgaccctgg 2400
cttacctgat cttctgcctg tgttccctcg tgggaattct ccatctgcaa cgcgctctcg 2460
tgctgcggcg caagcgcaag cggatgaccg acccaactag aaggttcttc aaggtcaccc 2520
cgccgcccgg ctcggggcca cagaatcagt acggcaacgt gctgtcactg ccgaccccca 2580
cttccggcct gggacgggca caacgctggg ccgcgggcct cggtggcacc gccccgtcct 2640
acggcaaccc gtcctccgac gtgcaagccg atggtgccct ggggtcccgc tcccctccgg 2700
gagtgggacc cgaagaagaa gagggagagg gttacgaaga acccgactca gaagaggact 2760
ccgaattcta cgaaaacgat agcaacctgg gacaggatca gctgtcccaa gacggatcgg 2820
gctacgagaa ccccgaggac gaacccctgg gaccagagga tgaggactcc ttctccaatg 2880
ctgagtcgta cgagaacgag gacgaagaac tgacacagcc agtggccagg accatggact 2940
tcctttcccc tcacggttcg gcgtgggacc cgtcccgcga ggccacctcc ctgggaagcc 3000
agtcatacga agatatgcgg ggcatcctct acgcggcgcc gcagcttaga tcgatccgag 3060
gacagcctgg acccaaccac gaagaggacg ccgactccta tgaaaacatg gacaaccctg 3120
atggacctga ccctgcctgg gggggtggcg gccggatggg cacctggtct acccggtaat 3180
gaggttaatt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 3240
ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 3300
aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 3360
tacaatagca ggcatgctgg ggatgcggtg ggctctatgg gtacccaggt gctgaagaat 3420
tgacccggtt cctcctgggt tataatggtt acaaataaag caatagcatc acaaatttca 3480
caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 3540
cttatcatgt ctggaagaca ccacaggtac caatattctc actgactccg tcctggagtt 3600
ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat 3660
ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca 3720
ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg 3780
cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga 3840
tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg 3900
acccagctgt atctctccag gcaagcactt ccaccccttc tgcttgcatc cagacaccat 3960
caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc 4020
catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc ttaaggctgt 4080
tctgaacgct gtgacttgga gagtgtccca gagccctcaa caccagcatg tcccacgtcc 4140
atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca 4200
aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata ggacaagggg 4260
gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc agtttttccc 4320
ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg 4380
caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttgatgacc 4440
ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa cccaggccgt 4500
tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc 4560
caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca 4620
ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc 4680
ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt 4740
acatccctgg gggctttggg ggggggctgt ccccgtgagc tcgcgctagc gacagccccc 4800
ccggctcgtt gcccagaggg gctgtcatcg ctagaaactc tctgcagcgg cggcacatgc 4860
tgccgctgca gagagaagaa ttaaaatgcc aacaacgtat gggacgcgtc gttggcattt 4920
aagccctttt actgccacga cgtatcccat gcgtgattgg caggcaaagg gttaaggcgc 4980
gccgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc 5040
aaggaaggca cgggggaggg gcaaacaaca gatggctggc aagagagcag gtttactgat 5100
aggtatcgag atcgacggcc ttgaccactt ccaccaggca catgtgatct ctcctctcat 5160
cgcggtcttt ggagagctta gtgtgataag tgatatgatg gtagcgcgga atgtggacag 5220
ccgctgaacc ggccagtggc cgattcatct ggctacactt ggtcaccagc ttctccgtca 5280
ccccctcgat gtcgtaggct tgattgaact ccacgcggat tccgttgttc acagtgtcgg 5340
ggagaatgta aaggatgctg ggagggcact ggaaggcgac attcttccga agaatatgcc 5400
cgtccttctt aaagttttct ccagtcagag tcacccggtt gtagatagat cccctctcat 5460
aggtgaccat cgcgcgggtc ttgtacactc catctccctc aaaagaaatg gtgcgctctt 5520
gggtataacc ttccggcatg gcggatttga agaagtcctt aatgtggcta gggtacttag 5580
caaaacactg cactccatac gagagggttg acacaagggt ggcccaaggc actggcagat 5640
ctccagtggt acagatatac ttggccttaa tggttccagt cgtagcgtcc ccggttcctt 5700
ctcccttgat gatgaacttc attccttcga cgtccccttc cagctcggtg atgtacggaa 5760
tctccttctc aaacagctta gcaccttcgg tcagggcagt catggtggcg gcgtcactct 5820
tggcacgggg aatccgcgtt ccaatgcacc gttcccggcc gcggaggctg gatcggtccc 5880
ggtgtcttct atggaggtca aaacagcgtg gatgggctct ccaggcgatc tgacggttca 5940
ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca 6000
atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac 6060
tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac 6120
gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga 6180
tgtactgcca agtaggaaag tcccgtaagg tcatgtactg ggcataatgc caggcgggcc 6240
atttaccgtc attgacgtca atagggggcg gacttggcat atgatacact tgatgtactg 6300
ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg 6360
gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc 6420
agccaggcgg gccatttacc gtaagtcgtc tacagagccc cgtacgccag agcccccgca 6480
gtgtcgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga 6540
agtaaaaaat gagccatatc caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg 6600
atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa 6660
tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta 6720
gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc 6780
cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg 6840
cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata 6900
ttgttgatgc gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc 6960
cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt 7020
tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga 7080
aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct 7140
cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag 7200
tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt 7260
ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata 7320
aattgcagtt tcatttgatg ctcgatgagt ttttctaact catgaccaaa atcccttaac 7380
gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 7440
ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 7500
accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 7560
cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt tagcccacca 7620
cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 7680
tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 7740
taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 7800
gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 7860
agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 7920
ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 7980
acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 8040
caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 8100
tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 8160
tcaaggctga gcggccgctt aaccctttca ctgccagccg atttgtccca aagtggaact 8220
tgtattgcca gacagttttt gaacgttttg cactgtttca ctttaggggc ctttcctcga 8280
ggggactttt agtttaccca ggaaaacaat atattgtttt tttcagaaca acctaagctt 8340
tcaaaatatg gtagaatttt tgtgtaattc caattctgta acaagatata ggcttctaaa 8400
tgtctaaaaa tgcaaaccta ggtggagtgt ggaactgaac ctccgtggga gtcttgagtg 8460
tgccaggccc tctctccgtg aaggaggcaa tgcctgtggg cgtcgccgtt gccgggacgg 8520
tctggcacac gcaggcgtgt ggctctcgtt catttccacg tagaagtcca gagcgacacc 8580
ccagagagga gatgcctccc cggcgtgatg gcctgacgat ggattcccgc gtgcggcaac 8640
gtggggagtc tgcagtgtgg ccggtttgga acctggcaag gagagcgaag gcaccgtgcc 8700
gggcttgcac ccttccctgc atgtttccgg gtgcccgcag agctccggga gcaaacagtc 8760
ggcatggcca gcctttcggg ggccggagag acttgagcaa caggccgcct tgcggagggc 8820
aaagccacgc ggaaaccaaa atcacgcctc cgtcgtcctg cgtgtggctc ctccgtggcc 8880
ggggctgtcg gcctcgcgcc gcgttgcagg gctcagcctg gggatgtgcg gtctgtgaac 8940
cgcgcgggtg aacacccgac ggcaacccga gtcccggtct tttgtcccgg aggaaaccgc 9000
ccactccctg ggccacggaa ccggggcgaa tgggtggtgc cccgccggcc ggcgcggcgg 9060
ctgtgggccc agccctcagc ccgcgccgga cgctgaccgt tttcccggag ggcgggggtc 9120
ccgctactcc cggaggccga ggaccgcttt tcctccctgc cttcctcccc ccgttccccg 9180
gctccctccc gcccgccccc agtccctgcg tcgctctgtc tttccctccg ttcctccctg 9240
cctccctgcc tccctccctc cctcctaacg tccctccgcc cgtccttccg cccctctagg 9300
actcccgttc ctctctccat ctctgcccgc cttccctccc gcctggaacg ctcagcgtcc 9360
ccggtgtgcg ccgggcctgg ggtctgcgtt ccgccgccag gcgctccgtg ctggcacctg 9420
ggcggctgca ggggcccggg cgggcgggcg acggtggcgc gggggcgcat aggaggcgag 9480
ccgccggagc ggtgtcaggc ccggacgctg cgcggggccc ggtgtttcgc gggacggggg 9540
tcaccaccca gcccagggga cgacgcgttt tccgggggtg gggggtgggg ggtggggagg 9600
gggcggtcag gcggcggggt gggctggtgg agaggcagga gagctctgcc cgggctgctc 9660
ccacagccca ggcggctgcc cgcaaacccg cgcgtgcgca gtaggcggcc cacctggtgt 9720
tcagcgaagg gcgacacaaa attttctgat ctccataaga gaagagggac agctatgact 9780
gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga aaattttagg 9840
gatgttaaag aaaaaaataa cacaaaacaa aatataaaaa aaatctaacc tcaagtcaag 9900
gcttttctat ggaataagga atggacagca gggggctgtt tcatatactg atgacctctt 9960
tatagccaac ctttgttcat ggcagccagc atatgggcat atgttgccaa actctaaacc 10020
aaatactcat tctgatgttt taaatgattt gccctcccat atgttcttcc gagtgagaga 10080
cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccattaatt 10140
aagctttt 10148
<210> 224
<211> 11424
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 224
agagtagtct tccctctccc ggccagagga gcaaggtatg cgggaggcga ccaggaggat 60
agcggggctg acgtcgggag gtggcctccg tgggaaggac acccggatct tgacacagcc 120
ttggcagcgg agtcaggaag agtaggggta ggttctggac gccctcttgg ccagctcatc 180
gccgccccac cctctgctgg agcacagagt aattcataca aaaggaggga tcgccttcgc 240
aaggggagag cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc 300
cacgacagcg cgaggagcat gcgcccaggg ctgagcgcgg gtagatcaga gcacacaagc 360
tcacagtccc cggcggtggg gggaggggcg cgctgagcgg gggccaggga gctggcgcgg 420
ggcaaactgg gaaagtggtg tcgtgtgctg gctccgccct cttcccgagg gtgggggaga 480
acggtatata agtgcggtag tcgccttgga cgttcttttt cgcaacgggt ttgccgtcag 540
aacgcaggtg agtggcgggt gtggcttccg cgggccccgg agctggagcc ctgctctgag 600
cgggccgggc tgatatgcga gtgtcgtccg cagggtttag ctgtgagcat tcccacttcg 660
agtggcgggc ggtgcggggg tgagagtgcg aggcctagcg gcaaccccgt agcctcgcct 720
cgtgtccggc ttgaggccta gcgtggtgtc cgccgccgcg tgccactccg gccgcactat 780
gcgttttttg tccttgctgc cctcgattgc cttccagcag catgggctaa caaagggagg 840
gtgtggggct cactcttaag gagcccatga agcttacgtt ggataggaat ggaagggcag 900
gaggggcgac tggggcccgc ccgccttcgg agcacatgtc cgacgccacc tggatggggc 960
gaggcctgtg gctttccgaa gcaatcgggc gtgagtttag cctacctggg ccatgtggcc 1020
ctagcactgg gcacggtctg gcctggcggt gccgcgttcc cttgcctccc aacaagggtg 1080
aggccgtccc gcccggcacc agttgcttgc gcggaaagat ggccgctccc ggggccctgt 1140
tgcaaggagc tcaaaatgga ggacgcggca gcccggtgga gcgggcgggt gagtcaccca 1200
cacaaaggaa gagggccttg cccctcgccg gccgctgctt cctgtgaccc cgtggtctat 1260
cggccgcata gtcacctcgg gcttctcttg agcaccgctc gtcgcggcgg ggggagggga 1320
tctaatggcg ttggagtttg ttcacatttg gtgggtggag actagtcagg ccagcctggc 1380
gctggaagtc attcttggaa tttgcccctt tgagtttgga gcgaggctaa ttctcaagcc 1440
tcttagcggt tcaaaggtat tttctaaacc cgtttccagg tgttgtgaaa gccaccgcta 1500
attcaaagca agccgccacc atgcccccgc cccgcctgct gttcttcctc ctcttcctga 1560
ccccaatgga agtccgccct gaggaaccgc tcgtcgtgaa agtggaagaa ggagacaacg 1620
ccgtgcttca gtgcctcaag ggcacctccg acgggccgac ccagcaactc acttggagca 1680
gagagagccc gctcaagccc ttcctgaaac tgagcttggg gcttccgggg ctgggtattc 1740
atatgcggcc cctcgcaatc tggttgttta tctttaacgt gtcacagcag atgggtggct 1800
tttacctgtg ccagcccggg cctccttcgg aaaaggcctg gcagcctgga tggactgtga 1860
atgtcgaagg ctcgggagag ctgttcagat ggaacgtgtc cgacctggga ggcttgggct 1920
gcggtctgaa gaacagatcc tccgaggggc cgagctcccc ctccggaaag ctgatgtcac 1980
ctaagctcta cgtgtgggct aaggacaggc ccgagatttg ggaaggggag ccgccttgtt 2040
tgcctcctcg ggactcactc aaccagtcgc tgagccagga tctcactatg gcccccgggt 2100
ccacgctgtg gctgtcctgc ggagtccccc cggactcagt gtcgagagga cccctgtcgt 2160
ggacgcacgt gcatccgaag ggccctaaga gcctgctgag ccttgagctg aaggacgacc 2220
gccccgcaag ggatatgtgg gtcatggaaa ccggactgct cctgccgcgg gcgaccgccc 2280
aagatgccgg aaagtattac tgccaccgcg gcaacctgac tatgagcttc cacctggaaa 2340
ttaccgcgcg gccagtgctg tggcactggc tgctgcggac tggcggatgg aaagtgtccg 2400
ccgtgaccct ggcttacctg atcttctgcc tgtgttccct cgtgggaatt ctccatctgc 2460
aacgcgctct cgtgctgcgg cgcaagcgca agcggatgac cgacccaact agaaggttct 2520
tcaaggtcac cccgccgccc ggctcggggc cacagaatca gtacggcaac gtgctgtcac 2580
tgccgacccc cacttccggc ctgggacggg cacaacgctg ggccgcgggc ctcggtggca 2640
ccgccccgtc ctacggcaac ccgtcctccg acgtgcaagc cgatggtgcc ctggggtccc 2700
gctcccctcc gggagtggga cccgaagaag aagagggaga gggttacgaa gaacccgact 2760
cagaagagga ctccgaattc tacgaaaacg atagcaacct gggacaggat cagctgtccc 2820
aagacggatc gggctacgag aaccccgagg acgaacccct gggaccagag gatgaggact 2880
ccttctccaa tgctgagtcg tacgagaacg aggacgaaga actgacacag ccagtggcca 2940
ggaccatgga cttcctttcc cctcacggtt cggcgtggga cccgtcccgc gaggccacct 3000
ccctgggaag ccagtcatac gaagatatgc ggggcatcct ctacgcggcg ccgcagctta 3060
gatcgatccg aggacagcct ggacccaacc acgaagagga cgccgactcc tatgaaaaca 3120
tggacaaccc tgatggacct gaccctgcct gggggggtgg cggccggatg ggcacctggt 3180
ctacccggta atgaggttaa ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 3240
ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 3300
cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 3360
gaggattggg aatacaatag caggcatgct ggggatgcgg tgggctctat gggtacccag 3420
gtgctgaaga attgacccgg ttcctcctgg ggaagacacc acaaggtacc aatattctca 3480
ctgactccgt cctggagttg gatgagagat aatggcctta cgttgtgcca ggggagggtc 3540
gggctggatt tagcaagatt taccttctcc aaagagcggt gctgcagtgg cacagctgcc 3600
cacggaggtg ggggggtcac cgtccctgga ggtgatgaag aactgtgggg atgtggcact 3660
gagggacatg gccagtgggc acggtgggtg ggttggggtt ggtcttgggg atcttggagg 3720
gcttttccag ccttcatgat ttgacgattg tatgaacatc tacatggcaa ttctccagct 3780
gcctgtccca gtcctactga cccagctgta tctctccagg caagcacttc caccccttct 3840
gcttgcatcc agacaccatc aaacatgcag gctcagacac agggaccagc agtgtctgtg 3900
gcctttttgt gctcctctcc atgctgggtt ttaacttgct ctttgtcctt ctatcctatc 3960
ttcttatcct taaggctgtt ctgaacgctg tgacttggag agtgtcccag agccctcaac 4020
accagcatgt cccacgtcca tgctgtcctg cacttcctta tccccaagat ctgcctctcc 4080
gtgatgcact gaattggcaa acatgtgtca ccccagacca acaatgtcac agcaaactcc 4140
cccttgatag gacaaggggg aatggcttta cactgagaca ggggaggttt gggttggata 4200
tgaggaggca gtttttcccc cagagggtgg tgacgcactg aacaggttgc ccaaggaggc 4260
tgtggatgcc ccatccctgc aggcattcaa ggccaggctg gatgtggctc tgggcagcct 4320
gggctgctgg ttgatgaccc tgcacatagc agggggttgg atctggatga gcactgtgct 4380
cctttgcaac ccaggccgtt ctatgattct gtcattctaa atctctcttt cagcctaaag 4440
ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga 4500
ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg 4560
gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta 4620
gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc cccgtgagct 4680
cgcgctaggt catattttta gtttaaaaaa ataattatat gttttataat gaaaagaatc 4740
tcattatctt tcagtattag gttgatttat attccaaaga ataatatttt tgttaaattg 4800
ttgatttttg taaacctcta aatgtttgtt gctaaaatta ctgtgtttaa gaaaaagatt 4860
aataaataat aataatttca taattaaaaa cttctttcat tgaatgccat taaataaacc 4920
attattttac aaaataagat caacataatt gagtaaataa taataagaac aatattatag 4980
tacaacaaaa tatgggtatg tcataccctg ccacattctt gatgtaactt tttttcacct 5040
catgctcgcc gggttaagtt taaacgatgc aatttcctca ttttattagg aaaggacagt 5100
gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg 5160
gcaattttct aattgagctt gtgaccaagc ttcgacggga gatcgcaata tcgtgccaca 5220
gcgtgttccc tctgaacgac gaacatctcg ttatcgctct cctccaggcg ttccagtcta 5280
tggtccaccg cgtggatacc cggcatcttc aggtttttgg ccggcttctt cgaccggtac 5340
gtagtcacaa agctacacga caaatgtcct cctccgacca gcttcagggc catgtcagac 5400
cggccttcga gtcctccgtc cgctgggtag agcatttcgg tattggcttc ccaccccaga 5460
gtctttttct gcatcacggg gccattggat gggaagttca cacctctgat cttcacgttg 5520
tagacaaggc agccatcttc caggctagtg tcttgcatca cggtcaccac gcctccatct 5580
tcgtaggtcg tgacccgttc ccaagtgaat ccctcaggga aggattgctt gaagaagtcc 5640
gggatcccct tcgggtactt gataaaagtg cgcgatccgt acatgaagga ggtagccagg 5700
atgtcgaatg caaaagggag tggtccgcct tcgaccacct taatgcgcat ggtctgggtc 5760
ccttcgtagg gctttccctc gccctctgag gtgcatttga agtggtgatt gttgacagtt 5820
ccctccatgt acagtttcat atgcatgttc tctttgatca attcctcccc ttttgacacc 5880
atggtggcgg cgtcactctt ggcacgggga atccgcgttc caatgcaccg ttcccggccg 5940
cggaggctgg atcggtcccg gtgtccacta tggaggtcaa aacagcgtgg atggcctctc 6000
caggcgatct gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc 6060
ctaccgccca tttgcgtcaa cggggcgggg ttattacgac attttggaaa gtcccgttga 6120
ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt 6180
gagtcaaacc gctatccacg cccattggtg tactgccaaa accgcatcag catggtaata 6240
gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccgtaaggt catgtactgg 6300
gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgg acttggcata 6360
tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt 6420
caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg 6480
cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagtgacgc cgaggagtcg 6540
ccgtttaaac ttaatcagcc ttgagcggta tcagctcact caaaggcggt aatacggtta 6600
tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6660
aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 6720
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6780
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6840
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 6900
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6960
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7020
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7080
ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag aagaacagta 7140
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7200
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7260
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7320
tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagttag aaaaactcat 7380
cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa 7440
aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat 7500
cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct 7560
cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga 7620
atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt 7680
catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgaggc 7740
gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgagtgc aaccggcgca 7800
ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct 7860
ggaacgctgt ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga 7920
taaaatgctt gatggtcgga agtggcataa attccgtcag ccagtttagt ctgaccatct 7980
catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat 8040
cgggcttccc atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc 8100
atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc 8160
gttggatatg gctcattttt tacttcctca ccttgtcgta ttatactatg ccgatatact 8220
atgccgatga ttaattgtcg acactgcggg ggctctttaa ttaacccggc gagcatgagg 8280
cagggtatct cataccctgg taaaatttta aagttgtgta ttttataaaa ttttcgtctg 8340
acaacactag cgcgctcagt agctggaggc aggagcgtgc gggaggggat agtggcgtga 8400
tcgcagtgtg gcacgggaca ccggcgagat attcgtgtgc aaacctgttt cgggtatgtt 8460
ataccctgcc tcattgttga cgtatttttt ttatgtaatt tttccgatta ttaatttcaa 8520
ctgttttatt ggtattttta tgttatccat tgttcttttt ttatgattta ctgtatcggt 8580
tgtctttcgt tcctttagtt gagttttttt ttattatttt cagtttttga tcaaagctag 8640
gaatattctc actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc 8700
aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg 8760
gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg 8820
gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg 8880
gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca 8940
attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag gcaagcactt 9000
ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag 9060
cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct 9120
tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca 9180
gagccctcaa caccagcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga 9240
tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca 9300
cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt 9360
tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg 9420
cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct 9480
ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg 9540
agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt 9600
tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag 9660
aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg 9720
ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct 9780
gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt 9840
ccccgtgagc tcgctctaga gatctccata agagaagagg gacagctatg actgggagta 9900
gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta 9960
aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc 10020
tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc 10080
aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact 10140
cattctgatg ttttaaatga tttgccctcc catatgttct tccgagtgag agacacaaaa 10200
aattccaaca cactattgca atgaaaataa atttccttta ttagccattt attcaaagac 10260
caggaagggc cggtgcaagg aggggaggag ggcccgttgg gaggcccagc gggcaggagg 10320
aacggctacc gaggctccag cttttggaga cgctacttga attgtttcag gaagcgcaga 10380
tcgttttcga aaaagctgcg caaatcggta acgccatagc gcagcatagt caggcgctcc 10440
atacccatac caaaaccaaa gccgctgtag acctcagggt caatgccgac attgcgcagg 10500
acattcgggt gaaccatgcc gcaacccaga acttccagcc acttaccatt cttacccatg 10560
acatcaactt ccgccgacgg ctccgtaaac ggaaagtagc tcggacggaa gcgaatctgc 10620
agatcctctt cgaaaaagtt acgcaggaaa tcatgcaggg tgcctttcag attggtaaaa 10680
gagatgttcg tatcaacaat caggccttcc atttgatgga acatcggggt gtgggtctgg 10740
tcgtaatcgt tgcgatacac gcgaccaggc gcgataatac gaatcggcgg ctgctgggct 10800
ttcatggtac ggatctgaac accggaagtt tgggtacgca gcagacgggt cgtatcgaac 10860
caaaaggtgt catggtcggc acgtgctggg tggtgacccg ggatgttcag agcgtcgaag 10920
ttgtggtagt catcctcgat ctccggaccc gtcgccacgg taaaacccag ttcgccgaag 10980
aagctctcaa tgcggtcgat ggtacgcgta accggatgca ggccaccgtt ctcgatacga 11040
cgacccggca ggctcacgtc gatcgtttct gcggccaaac gcgcgttcag cgcagcgctc 11100
tccagctcag ccttacgggc attcagcgcc tgttgcactt gctctttcgc ttcattaatc 11160
accgcaccgg ctgccggacg ttcttctggc ggcaactcac gcaaggtcgt catttgcagg 11220
gtcaggtggc cctttttacc cagatactcc acacggacat tatccagcgc tgccacgtcg 11280
cttgcctggc taattgctgc tttcgcgctc gctaccaatt ccgccaagtg ggacattttt 11340
ttacctccta tatacgagcc ggatgattaa ttgtcaacag ctcatttcag aatgtgtcgc 11400
ccttattcga ctcttttcgt ctct 11424
<210> 225
<211> 9773
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 225
atggcactcc ctgtcaccgc cctcctcctc ccactcgccc tcttgctgca cgccgctcgc 60
ccggatattc agatgacgca gaccacctca agtctgtcgg cctcccttgg tgatcgggtc 120
accatttcct gccgagccag ccaggacatc tccaagtacc tgaactggta ccagcagaag 180
cccgacggga ccgtgaagct gctgatctac catacctccc ggctgcatag cggggtgccg 240
tcaaggttta gcggatcggg atccggcacc gactactcgc tgactatctc caacttggaa 300
caagaggaca tcgccaccta cttctgtcaa caagggaata ctctgcccta cactttcggc 360
gggggaacca agctcgagat cactggcggc ggcggctcgg gcggtggtgg atccgggggc 420
ggtggctccg aggtcaagct tcaggaatcc ggacccggcc tggtggcacc gtcacaatcc 480
ctatccgtga catgcaccgt cagcggagtg tcgctgcccg attacggagt gtcttggatt 540
aggcagcccc cgcgcaaagg tcttgagtgg ctgggagtga tctggggatc agagactacc 600
tactacaaca gcgccctcaa gtcgaggctc accatcatca aggacaactc caagtcccaa 660
gtgtttctga agatgaactc cctgcaaact gacgacaccg ccatctacta ctgcgcgaag 720
cactactact acgggggaag ctacgctatg gactattggg gacagggaac ttccgtgact 780
gtgtccagca ccacgacacc agccccgcgc ccgccgaccc ccgccccgac cattgcgagc 840
cagccgctga gccttcggcc ggaagcctgc aggcccgcgg ccggcggagc cgtgcacacc 900
agaggactgg acttcgcctg cgatatctat atctgggcgc ctctggccgg aacctgtgga 960
gtcctgctgc tgtcactcgt gattactctg tactgcaagc gcggtcggaa gaagctgctc 1020
tacattttca agcaaccttt catgcggcca gtgcagacca ctcaggaaga agatggctgt 1080
tcctgccggt tccctgaaga agaagagggc ggctgcgaat tgagagtgaa gttctcccgc 1140
tcggctgacg ctcccgccta caaacagggg cagaaccagc tgtataacga actgaacctc 1200
gggcgccgcg aggaatacga cgtgctggac aagcggagag gccgcgatcc tgagatgggg 1260
ggaaagcccc ggagaaagaa ccctcaggag ggcctgtaca atgagctgca gaaagacaaa 1320
atggccgagg cgtacagcga gatcggcatg aagggcgaac gccggagagg aaagggacac 1380
gacggactgt accagggact gtccaccgcg accaaggata cctacgacgc cctgcacatg 1440
caggcactgc cacctcggtg ataaaagctt aattaatggc taataaagga aatttatttt 1500
cattgcaata gtgtgttgga attttttgtg tctctcactc ggaagaacat atgggagggc 1560
aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat 1620
gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc 1680
tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt 1740
ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc 1800
cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag 1860
atcagaaaat tttgtgtcgc ccttcgctga acaccaggtg ggccgcctac tgcgcacgcg 1920
cgggtttgcg ggcagccgcc tgggctgtgg gagcagcccg ggcagagctc tcctgcctct 1980
ccaccagccc accccgccgc ctgaccgccc cctccccacc ccccaccccc cacccccgga 2040
aaacgcgtcg tcccctgggc tgggtggtga cccccgtccc gcgaaacacc gggccccgcg 2100
cagcgtccgg gcctgacacc gctccggcgg ctcgcctcct atgcgccccc gcgccaccgt 2160
cgcccgcccg cccgggcccc tgcagccgcc caggtgccag cacggagcgc ctggcggcgg 2220
aacgcagacc ccaggcccgg cgcacaccgg ggacgctgag cgttccaggc gggagggaag 2280
gcgggcagag atggagagag gaacgggagt cctagagggg cggaaggacg ggcggaggga 2340
cgttaggagg gagggaggga ggcagggagg cagggaggaa cggagggaaa gacagagcga 2400
cgcagggact gggggcgggc gggagggagc cggggaacgg ggggaggaag gcagggagga 2460
aaagcggtcc tcggcctccg ggagtagcgg gacccccgcc ctccgggaaa acggtcagcg 2520
tccggcgcgg gctgagggct gggcccacag ccgccgcgcc ggccggcggg gcaccaccca 2580
ttcgccccgg ttccgtggcc cagggagtgg gcggtttcct ccgggacaaa agaccgggac 2640
tcgggttgcc gtcgggtgtt cacccgcgcg gttcacagac cgcacatccc caggctgagc 2700
cctgcaacgc ggcgcgaggc cgacagcccc ggccacggag gagccacacg caggacgacg 2760
gaggcgtgat tttggtttcc gcgtggcttt gccctccgca aggcggcctg ttgctcaagt 2820
ctctccggcc cccgaaaggc tggccatgcc gactgtttgc tcccggagct ctgcgggcac 2880
ccggaaacat gcagggaagg gtgcaagccc ggcacggtgc cttcgctctc cttgccaggt 2940
tccaaaccgg ccacactgca gactccccac gttgccgcac gcgggaatcc atcgtcaggc 3000
catcacgccg gggaggcatc tcctctctgg ggtgtcgctc tggacttcta cgtggaaatg 3060
aacgagagcc acacgcctgc gtgtgccaga ccgtcccggc aacggcgacg cccacaggca 3120
ttgcctcctt cacggagaga gggcctggca cactcaagac tcccacggag gttcagttcc 3180
acactccacc taggtcatat ttttagttta aaaaaataat tatatgtttt ataatgaaaa 3240
gaatctcatt atctttcagt attaggttga tttatattcc aaagaataat atttttgtta 3300
aattgttgat ttttgtaaac ctctaaatgt ttgttgctaa aattactgtg tttaagaaaa 3360
agattaataa ataataataa tttcataatt aaaaacttct ttcattgaat gccattaaat 3420
aaaccattat tttacaaaat aagatcaaca taattgagta aataataata agaacaatat 3480
tatagtacaa caaaatatgg gtatgtcata ccctgccaca ttcttgatgt aacttttttt 3540
cacctcatgc tcgccgggtt aagggctaca atgaactcga aacgaccggt ttgcattttt 3600
agacatttag aagcctatat cttgttacag aattggaatt acacaaaaat tctaccatat 3660
tttgaaagct taggttgttc tgaaaaaaac aatatattgt tttcctgggt aaactaaaag 3720
tcccctcgag gaaaggcccc taaagtgaaa cagtgcaaaa cgttcaaaaa ctgtctggca 3780
atacaagttc cactttgacc aaaacggctg gcagtaaaag ggttaagcgg ccgctcagcc 3840
ttgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 3900
cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 3960
gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 4020
aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 4080
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 4140
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 4200
ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 4260
cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 4320
agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4380
gaagtggtgg gctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 4440
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4500
tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4560
agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac 4620
tcacgttaag ggattttggt catgagttag aaaaactcat cgagcatcaa atgaaactgc 4680
aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa 4740
ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt 4800
ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca 4860
agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt 4920
tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca 4980
accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta 5040
aaaggacaat tacaaacagg aatcgagtgc aaccggcgca ggaacactgc cagcgcatca 5100
acaatatttt cacctgaatc aggatattct tctaatacct ggaacgctgt ttttccgggg 5160
atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga 5220
agtggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca 5280
acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga 5340
tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca 5400
gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttggatatg gctcattttt 5460
tacttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtcg 5520
acactgcggg ggctctggcg cgccttaacc tttttactgc caatgacgca tgggatacgt 5580
cgtggcagta aaagggctta aatgccaacg acgcgtccca tacgttgttg gcattttaat 5640
tcttctctct gcagcggcag catgtgccgc cgctgcagag agtttctagc gatgacagcc 5700
cctctgggca acgagccggg ggggctgtcc catgacgcgg ctagacatgc acgaccatta 5760
acccggcgag catgaggcag ggtatctcat accctggtaa aattttaaag ttgtgtattt 5820
tataaaattt tcgtctgaca acactagcgc gctcagtagc tggaggcagg agcgtgcggg 5880
aggggatagt ggcgtgatcg cagtgtggca cgggacaccg gcgagatatt cgtgtgcaaa 5940
cctgtttcgg gtatgttata ccctgcctca ttgttgacgt atttttttta tgtaattttt 6000
ccgattatta atttcaactg ttttattggt atttttatgt tatccattgt tcttttttta 6060
tgatttactg tatcggttgt ctttcgttcc tttagttgag ttttttttta ttattttcag 6120
tttttgatca aagctagcgc gagctcacgg ggacagcccc cccccaaagc ccccagggat 6180
gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 6240
ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 6300
ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 6360
tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga cagaatcata 6420
gaacggcctg ggttgcaaag gagcacagtg ctcatccaga tccaaccccc tgctatgtgc 6480
agggtcatca accagcagcc caggctgccc agagccacat ccagcctggc cttgaatgcc 6540
tgcagggatg gggcatccac agcctccttg ggcaacctgt tcagtgcgtc accaccctct 6600
gggggaaaaa ctgcctcctc atatccaacc caaacctccc ctgtctcagt gtaaagccat 6660
tcccccttgt cctatcaagg gggagtttgc tgtgacattg ttggtctggg gtgacacatg 6720
tttgccaatt cagtgcatca cggagaggca gatcttgggg ataaggaagt gcaggacagc 6780
atggacgtgg gacatgctgg tgttgagggc tctgggacac tctccaagtc acagcgttca 6840
gaacagcctt aaggataaga agataggata gaaggacaaa gagcaagtta aaacccagca 6900
tggagaggag cacaaaaagg ccacagacac tgctggtccc tgtgtctgag cctgcatgtt 6960
tgatggtgtc tggatgcaag cagaaggggt ggaagtgctt gcctggagag atacagctgg 7020
gtcagtagga ctgggacagg cagctggaga attgccatgt agatgttcat acaatcgtca 7080
aatcatgaag gctggaaaag ccctccaaga tccccaagac caaccccaac ccacccaccg 7140
tgcccactgg ccatgtccct cagtgccaca tccccacagt tcttcatcac ctccagggac 7200
ggtgaccccc ccacctccgt gggcagctgt gccactgcag caccgctctt tggagaaggt 7260
aaatcttgct aaatccagcc cgaccctccc ctggcacaac gtaaggccat tatctctcat 7320
ccaactccag gacggagtca gtgagaatat tggtaccggg gtgtggtgtc ttcttaacct 7380
cacccaggag gaaccgggtc aattcttcag cacctgggta cccatagagc ccaccgcatc 7440
cccagcatgc ctgctattgt attcccaatc ctcccccttg ctgtcctgcc ccaccccacc 7500
ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga 7560
aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac 7620
agatggctgg caagagagca ggtttactga taggtatcga gatcgacggc cttgaccact 7680
tccaccaggc acatgtgatc tctcctctca tcgcggtctt tggagagctt agtgtgataa 7740
gtgatatgat ggtagcgcgg aatgtggaca gccgctgaac cggccagtgg ccgattcatc 7800
tggctacact tggtcaccag cttctccgtc accccctcga tgtcgtaggc ttgattgaac 7860
tccacgcgga ttccgttgtt cacagtgtcg gggagaatgt aaaggatgct gggagggcac 7920
tggaaggcga cattcttccg aagaatatgc ccgtccttct taaagttttc tccagtcaga 7980
gtcacccggt tgtagataga tcccctctca taggtgacca tcgcgcgggt cttgtacact 8040
ccatctccct caaaagaaat ggtgcgctct tgggtataac cttccggcat ggcggatttg 8100
aagaagtcct taatgtggct agggtactta gcaaaacact gcactccata cgagagggtt 8160
gacacaaggg tggcccaagg cactggcaga tctccagtgg tacagatata cttggcctta 8220
atggttccag tcgtagcgtc cccggttcct tctcccttga tgatgaactt cattccttcg 8280
acgtcccctt ccagctcggt gatgtacgga atctccttct caaacagctt agcaccttcg 8340
gtcagggcag tcatggtggc ggcccttctg caaaaagaac aagcaagctc ttgtctatga 8400
gaagaggctg cgggagaaga aagtcagatt agcgtggcca gtcccctccc cccacccgcc 8460
tccattcccg ccgcatgccc catcacgtcc tctaccatcc tctgcaatgc ggagcctggg 8520
ctcggcttcc ggacctcgca ccagcgcccc taaacccgta cagcgtcctt ccccccattc 8580
ctaatttcca ttcctctccg gcctcctgcg gcccagtcct tttttttctt ttctttttca 8640
cctggcactg cacaagaaga tgcggctgtc tctagaacag ggaggagcag agagcaccag 8700
ggagggctgc agtccgtatt tataggaact ggatggtggg gggagctgat gacgcgcgcc 8760
ccgcctcccg ctcggctcat ccagtcccag ctcaagggcg cagaggcctg agctacgtgc 8820
acccgtaaag ccgcgagtag ctgggcctct ctcattcccc ccctccctct ttttggaccc 8880
gcctcgtttt tgaaatgtgc acgcaccaag cgtgtgggct ccgacctgag agggggaggg 8940
gagctgagat tgtcccgccg aggaagtgac cggatgaggg ttcccaggat aggactcagg 9000
gaatacagac tatggattaa ggacgaggag tccttggagt gtgcaccaag gacatccagg 9060
acccagaaac cagaaagctg cttcccgaat aaaatgggag aatctgagag ctgcaggaac 9120
cagggaaatg gagcagaagt gtgggtctgc ccaaggctgc cccatcgaac ttctccctat 9180
tagtgaaaag gcttaggaga agttgaggat cttctcgttg ttcttcggaa gactactctg 9240
gagacgtcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 9300
cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 9360
ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 9420
ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 9480
tggaagtagc acgactcact agtctcgtgc agatggacag caccgctgag caatggaagc 9540
gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgagagcagc 9600
ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg 9660
cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtccgtctc aggggcagct 9720
tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggccgcc acc 9773
<210> 226
<211> 11286
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 226
atggcactcc ctgtcaccgc cctcctcctc ccactcgccc tcttgctgca cgccgctcgc 60
ccggatattc agatgacgca gaccacctca agtctgtcgg cctcccttgg tgatcgggtc 120
accatttcct gccgagccag ccaggacatc tccaagtacc tgaactggta ccagcagaag 180
cccgacggga ccgtgaagct gctgatctac catacctccc ggctgcatag cggggtgccg 240
tcaaggttta gcggatcggg atccggcacc gactactcgc tgactatctc caacttggaa 300
caagaggaca tcgccaccta cttctgtcaa caagggaata ctctgcccta cactttcggc 360
gggggaacca agctcgagat cactggcggc ggcggctcgg gcggtggtgg atccgggggc 420
ggtggctccg aggtcaagct tcaggaatcc ggacccggcc tggtggcacc gtcacaatcc 480
ctatccgtga catgcaccgt cagcggagtg tcgctgcccg attacggagt gtcttggatt 540
aggcagcccc cgcgcaaagg tcttgagtgg ctgggagtga tctggggatc agagactacc 600
tactacaaca gcgccctcaa gtcgaggctc accatcatca aggacaactc caagtcccaa 660
gtgtttctga agatgaactc cctgcaaact gacgacaccg ccatctacta ctgcgcgaag 720
cactactact acgggggaag ctacgctatg gactattggg gacagggaac ttccgtgact 780
gtgtccagca ccacgacacc agccccgcgc ccgccgaccc ccgccccgac cattgcgagc 840
cagccgctga gccttcggcc ggaagcctgc aggcccgcgg ccggcggagc cgtgcacacc 900
agaggactgg acttcgcctg cgatatctat atctgggcgc ctctggccgg aacctgtgga 960
gtcctgctgc tgtcactcgt gattactctg tactgcaagc gcggtcggaa gaagctgctc 1020
tacattttca agcaaccttt catgcggcca gtgcagacca ctcaggaaga agatggctgt 1080
tcctgccggt tccctgaaga agaagagggc ggctgcgaat tgagagtgaa gttctcccgc 1140
tcggctgacg ctcccgccta caaacagggg cagaaccagc tgtataacga actgaacctc 1200
gggcgccgcg aggaatacga cgtgctggac aagcggagag gccgcgatcc tgagatgggg 1260
ggaaagcccc ggagaaagaa ccctcaggag ggcctgtaca atgagctgca gaaagacaaa 1320
atggccgagg cgtacagcga gatcggcatg aagggcgaac gccggagagg aaagggacac 1380
gacggactgt accagggact gtccaccgcg accaaggata cctacgacgc cctgcacatg 1440
caggcactgc cacctcggtg ataaaaatta atggctaata aaggaaattt attttcattg 1500
caatagtgtg ttggaatttt ttgtgtctct cactcggaag aacatatggg agggcaaatc 1560
atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 1620
ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 1680
tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 1740
ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 1800
ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatcag 1860
aaaattttgt gtcgcccttc gctgaacacc aggtgggccg cctactgcgc acgcgcgggt 1920
ttgcgggcag ccgcctgggc tgtgggagca gcccgggcag agctctcctg cctctccacc 1980
agcccacccc gccgcctgac cgccccctcc ccacccccca ccccccaccc ccggaaaacg 2040
cgtcgtcccc tgggctgggt ggtgaccccc gtcccgcgaa acaccgggcc ccgcgcagcg 2100
tccgggcctg acaccgctcc ggcggctcgc ctcctatgcg cccccgcgcc accgtcgccc 2160
gcccgcccgg gcccctgcag ccgcccaggt gccagcacgg agcgcctggc ggcggaacgc 2220
agaccccagg cccggcgcac accggggacg ctgagcgttc caggcgggag ggaaggcggg 2280
cagagatgga gagaggaacg ggagtcctag aggggcggaa ggacgggcgg agggacgtta 2340
ggagggaggg agggaggcag ggaggcaggg aggaacggag ggaaagacag agcgacgcag 2400
ggactggggg cgggcgggag ggagccgggg aacgggggga ggaaggcagg gaggaaaagc 2460
ggtcctcggc ctccgggagt agcgggaccc ccgccctccg ggaaaacggt cagcgtccgg 2520
cgcgggctga gggctgggcc cacagccgcc gcgccggccg gcggggcacc acccattcgc 2580
cccggttccg tggcccaggg agtgggcggt ttcctccggg acaaaagacc gggactcggg 2640
ttgccgtcgg gtgttcaccc gcgcggttca cagaccgcac atccccaggc tgagccctgc 2700
aacgcggcgc gaggccgaca gccccggcca cggaggagcc acacgcagga cgacggaggc 2760
gtgattttgg tttccgcgtg gctttgccct ccgcaaggcg gcctgttgct caagtctctc 2820
cggcccccga aaggctggcc atgccgactg tttgctcccg gagctctgcg ggcacccgga 2880
aacatgcagg gaagggtgca agcccggcac ggtgccttcg ctctccttgc caggttccaa 2940
accggccaca ctgcagactc cccacgttgc cgcacgcggg aatccatcgt caggccatca 3000
cgccggggag gcatctcctc tctggggtgt cgctctggac ttctacgtgg aaatgaacga 3060
gagccacacg cctgcgtgtg ccagaccgtc ccggcaacgg cgacgcccac aggcattgcc 3120
tccttcacgg agagagggcc tggcacactc aagactccca cggaggttca gttccacact 3180
ccacctaggt catattttta gtttaaaaaa ataattatat gttttataat gaaaagaatc 3240
tcattatctt tcagtattag gttgatttat attccaaaga ataatatttt tgttaaattg 3300
ttgatttttg taaacctcta aatgtttgtt gctaaaatta ctgtgtttaa gaaaaagatt 3360
aataaataat aataatttca taattaaaaa cttctttcat tgaatgccat taaataaacc 3420
attattttac aaaataagat caacataatt gagtaaataa taataagaac aatattatag 3480
tacaacaaaa tatgggtatg tcataccctg ccacattctt gatgtaactt tttttcacct 3540
catgctcgcc gggttaaggg ctacaatgaa ctcgaaacga ccggtttgca tttttagaca 3600
tttagaagcc tatatcttgt tacagaattg gaattacaca aaaattctac catattttga 3660
aagcttaggt tgttctgaaa aaaacaatat attgttttcc tgggtaaact aaaagtcccc 3720
tcgaggaaag gcccctaaag tgaaacagtg caaaacgttc aaaaactgtc tggcaataca 3780
agttccactt tgaccaaaac ggctggcagt aaaagggtta agcggccgct cagccttgag 3840
cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3900
gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3960
tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 4020
agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 4080
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4140
cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4200
ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4260
ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4320
ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4380
ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 4440
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4500
gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4560
atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc gtaactcacg 4620
ttaagggatt ttggtcatga gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt 4680
attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga 4740
aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac 4800
tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga 4860
gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt 4920
ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa 4980
accgttattc attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg 5040
acaattacaa acaggaatcg agtgcaaccg gcgcaggaac actgccagcg catcaacaat 5100
attttcacct gaatcaggat attcttctaa tacctggaac gctgtttttc cggggatcgc 5160
agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagtgg 5220
cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct 5280
acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca agcgatagat 5340
tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc 5400
catgttggaa tttaatcgcg gcctcgacgt ttcccgttgg atatggctca ttttttactt 5460
cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat tgtcgacact 5520
gcgggggctc tggcgcgcct taaccttttt actgccaatg acgcatggga tacgtcgtgg 5580
cagtaaaagg gcttaaatgc caacgacgcg tcccatacgt tgttggcatt ttaattcttc 5640
tctctgcagc ggcagcatgt gccgccgctg cagagagttt ctagcgatga cagcccctct 5700
gggcaacgag ccgggggggc tgtcccatga cgcggctaga catgcacgac cattaacccg 5760
gcgagcatga ggcagggtat ctcataccct ggtaaaattt taaagttgtg tattttataa 5820
aattttcgtc tgacaacact agcgcgctca gtagctggag gcaggagcgt gcgggagggg 5880
atagtggcgt gatcgcagtg tggcacggga caccggcgag atattcgtgt gcaaacctgt 5940
ttcgggtatg ttataccctg cctcattgtt gacgtatttt ttttatgtaa tttttccgat 6000
tattaatttc aactgtttta ttggtatttt tatgttatcc attgttcttt ttttatgatt 6060
tactgtatcg gttgtctttc gttcctttag ttgagttttt ttttattatt ttcagttttt 6120
gatcaaagct agcgcgagct cacggggaca gccccccccc aaagccccca gggatgtaat 6180
tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc 6240
gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg 6300
cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg 6360
ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg 6420
gcctgggttg caaaggagca cagtgctcat ccagatccaa ccccctgcta tgtgcagggt 6480
catcaaccag cagcccaggc tgcccagagc cacatccagc ctggccttga atgcctgcag 6540
ggatggggca tccacagcct ccttgggcaa cctgttcagt gcgtcaccac cctctggggg 6600
aaaaactgcc tcctcatatc caacccaaac ctcccctgtc tcagtgtaaa gccattcccc 6660
cttgtcctat caagggggag tttgctgtga cattgttggt ctggggtgac acatgtttgc 6720
caattcagtg catcacggag aggcagatct tggggataag gaagtgcagg acagcatgga 6780
cgtgggacat gctggtgttg agggctctgg gacactctcc aagtcacagc gttcagaaca 6840
gccttaagga taagaagata ggatagaagg acaaagagca agttaaaacc cagcatggag 6900
aggagcacaa aaaggccaca gacactgctg gtccctgtgt ctgagcctgc atgtttgatg 6960
gtgtctggat gcaagcagaa ggggtggaag tgcttgcctg gagagataca gctgggtcag 7020
taggactggg acaggcagct ggagaattgc catgtagatg ttcatacaat cgtcaaatca 7080
tgaaggctgg aaaagccctc caagatcccc aagaccaacc ccaacccacc caccgtgccc 7140
actggccatg tccctcagtg ccacatcccc acagttcttc atcacctcca gggacggtga 7200
cccccccacc tccgtgggca gctgtgccac tgcagcaccg ctctttggag aaggtaaatc 7260
ttgctaaatc cagcccgacc ctcccctggc acaacgtaag gccattatct ctcatccaac 7320
tccaggacgg agtcagtgag aatattggta ccggggtgtg gtgtcttctt aacctcaccc 7380
aggaggaacc gggtcaattc ttcagcacct gggtacccat agagcccacc gcatccccag 7440
catgcctgct attgtattcc caatcctccc ccttgctgtc ctgccccacc ccacccccca 7500
gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga 7560
cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg 7620
gctggcaaga gagcaggttt actgataggt atcgagatcg acggccttga ccacttccac 7680
caggcacatg tgatctctcc tctcatcgcg gtctttggag agcttagtgt gataagtgat 7740
atgatggtag cgcggaatgt ggacagccgc tgaaccggcc agtggccgat tcatctggct 7800
acacttggtc accagcttct ccgtcacccc ctcgatgtcg taggcttgat tgaactccac 7860
gcggattccg ttgttcacag tgtcggggag aatgtaaagg atgctgggag ggcactggaa 7920
ggcgacattc ttccgaagaa tatgcccgtc cttcttaaag ttttctccag tcagagtcac 7980
ccggttgtag atagatcccc tctcataggt gaccatcgcg cgggtcttgt acactccatc 8040
tccctcaaaa gaaatggtgc gctcttgggt ataaccttcc ggcatggcgg atttgaagaa 8100
gtccttaatg tggctagggt acttagcaaa acactgcact ccatacgaga gggttgacac 8160
aagggtggcc caaggcactg gcagatctcc agtggtacag atatacttgg ccttaatggt 8220
tccagtcgta gcgtccccgg ttccttctcc cttgatgatg aacttcattc cttcgacgtc 8280
cccttccagc tcggtgatgt acggaatctc cttctcaaac agcttagcac cttcggtcag 8340
ggcagtcatg gtggcggccc ttctgcaaaa agaacaagca agctcttgtc tatgagaaga 8400
ggctgcggga gaagaaagtc agattagcgt ggccagtccc ctccccccac ccgcctccat 8460
tcccgccgca tgccccatca cgtcctctac catcctctgc aatgcggagc ctgggctcgg 8520
cttccggacc tcgcaccagc gcccctaaac ccgtacagcg tccttccccc cattcctaat 8580
ttccattcct ctccggcctc ctgcggccca gtcctttttt ttcttttctt tttcacctgg 8640
cactgcacaa gaagatgcgg ctgtctctag aacagggagg agcagagagc accagggagg 8700
gctgcagtcc gtatttatag gaactggatg gtggggggag ctgatgacgc gcgccccgcc 8760
tcccgctcgg ctcatccagt cccagctcaa gggcgcagag gcctgagcta cgtgcacccg 8820
taaagccgcg agtagctggg cctctctcat tccccccctc cctctttttg gacccgcctc 8880
gtttttgaaa tgtgcacgca ccaagcgtgt gggctccgac ctgagagggg gaggggagct 8940
gagattgtcc cgccgaggaa gtgaccggat gagggttccc aggataggac tcagggaata 9000
cagactatgg attaaggacg aggagtcctt ggagtgtgca ccaaggacat ccaggaccca 9060
gaaaccagaa agctgcttcc cgaataaaat gggagaatct gagagctgca ggaaccaggg 9120
aaatggagca gaagtgtggg tctgcccaag gctgccccat cgaacttctc cctattagtg 9180
aaaaggctta ggagaagttg aggatcttct cgttgttctt cggaagacta ctctggagac 9240
gtctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc 9300
ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc 9360
ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc 9420
ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa 9480
gtagcacgac tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta 9540
ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgaga gcagcggccg 9600
ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc 9660
gcggtgttcc gcattctgca agcctccgga gcgcacgtcc gtctcagggg cagcttgctt 9720
gttctttttg cagaagctca gaataaacgc tcaactttgg ccgccaccat gggagcaccc 9780
accctgcctc ccgcttggca accgttcctg aaagaccacc ggatttcgac cttcaagaat 9840
tggccgttcc tcgagggctg tgcctgcact cctgagcgga tggccgaggc cgggttcatc 9900
cactgtccaa ccgagaacga acctgacctg gcccagtgct tcttctgctt taaggaactt 9960
gagggttggg agccggacga tgaccccatc gaagaacaca agaagcattc ctccggctgc 10020
gccttcctga gcgtgaagaa acagttcgaa gaactgactc tgggagagtt cttgaagctc 10080
gacagagagc gcgccaagaa caagatcgcg aaggaaacca acaacaagaa gaaagaattt 10140
gaggaaaccg cgaagaaggt ccgcagggcg attgaacagc tggctgccat ggattgataa 10200
aagcttaatg ctggagcctc ggtagccgtt cctcctgccc gctgggcctc ccaacgggcc 10260
ctcctcccct ccttgcaccg gcccttcctg gtctttgaat aaagtttacc tgactcatgg 10320
tcctttcact ttcacatggg atttcccagt tatgaaatta ataaaaatca gtgatttcca 10380
catctgtgtg tgcctgtgcc aggctgggtg gggaacagga ggccgagatg attccgggaa 10440
ctgtcagaag gaatcaatga tttccacatc ctgtctgtct tatgtcttgg ggggtgggga 10500
ggccaggaag attccaggaa ggtcagagtc aatcaatggt ttccacatct ctcagtgcct 10560
ctatctggag gccaggtagg gctggccttg ggggaggggg aggccagaat gactccaaga 10620
gctacaggaa ggggggaggg ggaaaaaggc cagaataatt ccaggaaccg ccaagaagat 10680
gacgtcgagg agaagttccc caactttccc gcctctcagc ctttgaaaga aagaaagggg 10740
agggggcagg ccgcgtgcag ccgcgagcgg tgctgggctc cggctccaat tccccatctc 10800
agtcgttccc aaagtcctcc tgtttcatcc aagcgtgtaa gggtccccgt ccttgactcc 10860
ctagtgtcct gctgcccaca gtccagtcct gggaaccagc accgatcacc tcccatcggg 10920
ccaatctcag tcccttcccc cctacgtcgg ggcccacacg ctcggtgcgt gcccagttga 10980
accaggcggc tgcggaaaaa aaaaagcggg gagaaagtag ggcccggcta ctagcggttt 11040
tacgggcgca cgtagctcag gcctcaagac cttgggctgg gactggctga gcctggcggg 11100
aggcggggtc cgagtcaccg cctgccgccg cgcccccggt ttctataaat tgagcccgca 11160
gcctcccgct tcgctctctg ctcctcctgt tcgacagtca gccgcatctt cttttgcgtc 11220
gccaggggga gcttgcttgt tctttttgca gaagctcaga ataaacgctc aactttggcc 11280
gccacc 11286
<210> 227
<211> 11520
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 227
atggcactcc ctgtcaccgc cctcctcctc ccactcgccc tcttgctgca cgccgctcgc 60
ccggatattc agatgacgca gaccacctca agtctgtcgg cctcccttgg tgatcgggtc 120
accatttcct gccgagccag ccaggacatc tccaagtacc tgaactggta ccagcagaag 180
cccgacggga ccgtgaagct gctgatctac catacctccc ggctgcatag cggggtgccg 240
tcaaggttta gcggatcggg atccggcacc gactactcgc tgactatctc caacttggaa 300
caagaggaca tcgccaccta cttctgtcaa caagggaata ctctgcccta cactttcggc 360
gggggaacca agctcgagat cactggcggc ggcggctcgg gcggtggtgg atccgggggc 420
ggtggctccg aggtcaagct tcaggaatcc ggacccggcc tggtggcacc gtcacaatcc 480
ctatccgtga catgcaccgt cagcggagtg tcgctgcccg attacggagt gtcttggatt 540
aggcagcccc cgcgcaaagg tcttgagtgg ctgggagtga tctggggatc agagactacc 600
tactacaaca gcgccctcaa gtcgaggctc accatcatca aggacaactc caagtcccaa 660
gtgtttctga agatgaactc cctgcaaact gacgacaccg ccatctacta ctgcgcgaag 720
cactactact acgggggaag ctacgctatg gactattggg gacagggaac ttccgtgact 780
gtgtccagca ccacgacacc agccccgcgc ccgccgaccc ccgccccgac cattgcgagc 840
cagccgctga gccttcggcc ggaagcctgc aggcccgcgg ccggcggagc cgtgcacacc 900
agaggactgg acttcgcctg cgatatctat atctgggcgc ctctggccgg aacctgtgga 960
gtcctgctgc tgtcactcgt gattactctg tactgcaagc gcggtcggaa gaagctgctc 1020
tacattttca agcaaccttt catgcggcca gtgcagacca ctcaggaaga agatggctgt 1080
tcctgccggt tccctgaaga agaagagggc ggctgcgaat tgagagtgaa gttctcccgc 1140
tcggctgacg ctcccgccta caaacagggg cagaaccagc tgtataacga actgaacctc 1200
gggcgccgcg aggaatacga cgtgctggac aagcggagag gccgcgatcc tgagatgggg 1260
ggaaagcccc ggagaaagaa ccctcaggag ggcctgtaca atgagctgca gaaagacaaa 1320
atggccgagg cgtacagcga gatcggcatg aagggcgaac gccggagagg aaagggacac 1380
gacggactgt accagggact gtccaccgcg accaaggata cctacgacgc cctgcacatg 1440
caggcactgc cacctcggtg ataaaaatta atggctaata aaggaaattt attttcattg 1500
caatagtgtg ttggaatttt ttgtgtctct cactcggaag aacatatggg agggcaaatc 1560
atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 1620
ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 1680
tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 1740
ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 1800
ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatcag 1860
aaaattttgt gtcgcccttc gctgaacacc aggtgggccg cctactgcgc acgcgcgggt 1920
ttgcgggcag ccgcctgggc tgtgggagca gcccgggcag agctctcctg cctctccacc 1980
agcccacccc gccgcctgac cgccccctcc ccacccccca ccccccaccc ccggaaaacg 2040
cgtcgtcccc tgggctgggt ggtgaccccc gtcccgcgaa acaccgggcc ccgcgcagcg 2100
tccgggcctg acaccgctcc ggcggctcgc ctcctatgcg cccccgcgcc accgtcgccc 2160
gcccgcccgg gcccctgcag ccgcccaggt gccagcacgg agcgcctggc ggcggaacgc 2220
agaccccagg cccggcgcac accggggacg ctgagcgttc caggcgggag ggaaggcggg 2280
cagagatgga gagaggaacg ggagtcctag aggggcggaa ggacgggcgg agggacgtta 2340
ggagggaggg agggaggcag ggaggcaggg aggaacggag ggaaagacag agcgacgcag 2400
ggactggggg cgggcgggag ggagccgggg aacgggggga ggaaggcagg gaggaaaagc 2460
ggtcctcggc ctccgggagt agcgggaccc ccgccctccg ggaaaacggt cagcgtccgg 2520
cgcgggctga gggctgggcc cacagccgcc gcgccggccg gcggggcacc acccattcgc 2580
cccggttccg tggcccaggg agtgggcggt ttcctccggg acaaaagacc gggactcggg 2640
ttgccgtcgg gtgttcaccc gcgcggttca cagaccgcac atccccaggc tgagccctgc 2700
aacgcggcgc gaggccgaca gccccggcca cggaggagcc acacgcagga cgacggaggc 2760
gtgattttgg tttccgcgtg gctttgccct ccgcaaggcg gcctgttgct caagtctctc 2820
cggcccccga aaggctggcc atgccgactg tttgctcccg gagctctgcg ggcacccgga 2880
aacatgcagg gaagggtgca agcccggcac ggtgccttcg ctctccttgc caggttccaa 2940
accggccaca ctgcagactc cccacgttgc cgcacgcggg aatccatcgt caggccatca 3000
cgccggggag gcatctcctc tctggggtgt cgctctggac ttctacgtgg aaatgaacga 3060
gagccacacg cctgcgtgtg ccagaccgtc ccggcaacgg cgacgcccac aggcattgcc 3120
tccttcacgg agagagggcc tggcacactc aagactccca cggaggttca gttccacact 3180
ccacctaggt catattttta gtttaaaaaa ataattatat gttttataat gaaaagaatc 3240
tcattatctt tcagtattag gttgatttat attccaaaga ataatatttt tgttaaattg 3300
ttgatttttg taaacctcta aatgtttgtt gctaaaatta ctgtgtttaa gaaaaagatt 3360
aataaataat aataatttca taattaaaaa cttctttcat tgaatgccat taaataaacc 3420
attattttac aaaataagat caacataatt gagtaaataa taataagaac aatattatag 3480
tacaacaaaa tatgggtatg tcataccctg ccacattctt gatgtaactt tttttcacct 3540
catgctcgcc gggttaaggg ctacaatgaa ctcgaaacga ccggtttgca tttttagaca 3600
tttagaagcc tatatcttgt tacagaattg gaattacaca aaaattctac catattttga 3660
aagcttaggt tgttctgaaa aaaacaatat attgttttcc tgggtaaact aaaagtcccc 3720
tcgaggaaag gcccctaaag tgaaacagtg caaaacgttc aaaaactgtc tggcaataca 3780
agttccactt tgaccaaaac ggctggcagt aaaagggtta agcggccgct cagccttgag 3840
cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3900
gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3960
tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 4020
agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 4080
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4140
cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4200
ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4260
ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4320
ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4380
ggtgggctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 4440
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4500
gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4560
atcctttgat cttttctacg gggtctgacg ctcagtggaa cgacgcgcgc gtaactcacg 4620
ttaagggatt ttggtcatga gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt 4680
attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga 4740
aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac 4800
tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga 4860
gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt 4920
ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa 4980
accgttattc attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg 5040
acaattacaa acaggaatcg agtgcaaccg gcgcaggaac actgccagcg catcaacaat 5100
attttcacct gaatcaggat attcttctaa tacctggaac gctgtttttc cggggatcgc 5160
agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagtgg 5220
cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct 5280
acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca agcgatagat 5340
tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc 5400
catgttggaa tttaatcgcg gcctcgacgt ttcccgttgg atatggctca ttttttactt 5460
cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat tgtcgacact 5520
gcgggggctc tggcgcgcct taaccttttt actgccaatg acgcatggga tacgtcgtgg 5580
cagtaaaagg gcttaaatgc caacgacgcg tcccatacgt tgttggcatt ttaattcttc 5640
tctctgcagc ggcagcatgt gccgccgctg cagagagttt ctagcgatga cagcccctct 5700
gggcaacgag ccgggggggc tgtcccatga cgcggctaga catgcacgac cattaacccg 5760
gcgagcatga ggcagggtat ctcataccct ggtaaaattt taaagttgtg tattttataa 5820
aattttcgtc tgacaacact agcgcgctca gtagctggag gcaggagcgt gcgggagggg 5880
atagtggcgt gatcgcagtg tggcacggga caccggcgag atattcgtgt gcaaacctgt 5940
ttcgggtatg ttataccctg cctcattgtt gacgtatttt ttttatgtaa tttttccgat 6000
tattaatttc aactgtttta ttggtatttt tatgttatcc attgttcttt ttttatgatt 6060
tactgtatcg gttgtctttc gttcctttag ttgagttttt ttttattatt ttcagttttt 6120
gatcaaagct agcgcgagct cacggggaca gccccccccc aaagccccca gggatgtaat 6180
tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc 6240
gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg 6300
cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg 6360
ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg 6420
gcctgggttg caaaggagca cagtgctcat ccagatccaa ccccctgcta tgtgcagggt 6480
catcaaccag cagcccaggc tgcccagagc cacatccagc ctggccttga atgcctgcag 6540
ggatggggca tccacagcct ccttgggcaa cctgttcagt gcgtcaccac cctctggggg 6600
aaaaactgcc tcctcatatc caacccaaac ctcccctgtc tcagtgtaaa gccattcccc 6660
cttgtcctat caagggggag tttgctgtga cattgttggt ctggggtgac acatgtttgc 6720
caattcagtg catcacggag aggcagatct tggggataag gaagtgcagg acagcatgga 6780
cgtgggacat gctggtgttg agggctctgg gacactctcc aagtcacagc gttcagaaca 6840
gccttaagga taagaagata ggatagaagg acaaagagca agttaaaacc cagcatggag 6900
aggagcacaa aaaggccaca gacactgctg gtccctgtgt ctgagcctgc atgtttgatg 6960
gtgtctggat gcaagcagaa ggggtggaag tgcttgcctg gagagataca gctgggtcag 7020
taggactggg acaggcagct ggagaattgc catgtagatg ttcatacaat cgtcaaatca 7080
tgaaggctgg aaaagccctc caagatcccc aagaccaacc ccaacccacc caccgtgccc 7140
actggccatg tccctcagtg ccacatcccc acagttcttc atcacctcca gggacggtga 7200
cccccccacc tccgtgggca gctgtgccac tgcagcaccg ctctttggag aaggtaaatc 7260
ttgctaaatc cagcccgacc ctcccctggc acaacgtaag gccattatct ctcatccaac 7320
tccaggacgg agtcagtgag aatattggta ccggggtgtg gtgtcttctt aacctcaccc 7380
aggaggaacc gggtcaattc ttcagcacct gggtacccat agagcccacc gcatccccag 7440
catgcctgct attgtattcc caatcctccc ccttgctgtc ctgccccacc ccacccccca 7500
gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga 7560
cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg 7620
gctggcaaga gagcaggttt actgataggt atcgagatcg acggccttga ccacttccac 7680
caggcacatg tgatctctcc tctcatcgcg gtctttggag agcttagtgt gataagtgat 7740
atgatggtag cgcggaatgt ggacagccgc tgaaccggcc agtggccgat tcatctggct 7800
acacttggtc accagcttct ccgtcacccc ctcgatgtcg taggcttgat tgaactccac 7860
gcggattccg ttgttcacag tgtcggggag aatgtaaagg atgctgggag ggcactggaa 7920
ggcgacattc ttccgaagaa tatgcccgtc cttcttaaag ttttctccag tcagagtcac 7980
ccggttgtag atagatcccc tctcataggt gaccatcgcg cgggtcttgt acactccatc 8040
tccctcaaaa gaaatggtgc gctcttgggt ataaccttcc ggcatggcgg atttgaagaa 8100
gtccttaatg tggctagggt acttagcaaa acactgcact ccatacgaga gggttgacac 8160
aagggtggcc caaggcactg gcagatctcc agtggtacag atatacttgg ccttaatggt 8220
tccagtcgta gcgtccccgg ttccttctcc cttgatgatg aacttcattc cttcgacgtc 8280
cccttccagc tcggtgatgt acggaatctc cttctcaaac agcttagcac cttcggtcag 8340
ggcagtcatg gtggcggccc ttctgcaaaa agaacaagca agctcttgtc tatgagaaga 8400
ggctgcggga gaagaaagtc agattagcgt ggccagtccc ctccccccac ccgcctccat 8460
tcccgccgca tgccccatca cgtcctctac catcctctgc aatgcggagc ctgggctcgg 8520
cttccggacc tcgcaccagc gcccctaaac ccgtacagcg tccttccccc cattcctaat 8580
ttccattcct ctccggcctc ctgcggccca gtcctttttt ttcttttctt tttcacctgg 8640
cactgcacaa gaagatgcgg ctgtctctag aacagggagg agcagagagc accagggagg 8700
gctgcagtcc gtatttatag gaactggatg gtggggggag ctgatgacgc gcgccccgcc 8760
tcccgctcgg ctcatccagt cccagctcaa gggcgcagag gcctgagcta cgtgcacccg 8820
taaagccgcg agtagctggg cctctctcat tccccccctc cctctttttg gacccgcctc 8880
gtttttgaaa tgtgcacgca ccaagcgtgt gggctccgac ctgagagggg gaggggagct 8940
gagattgtcc cgccgaggaa gtgaccggat gagggttccc aggataggac tcagggaata 9000
cagactatgg attaaggacg aggagtcctt ggagtgtgca ccaaggacat ccaggaccca 9060
gaaaccagaa agctgcttcc cgaataaaat gggagaatct gagagctgca ggaaccaggg 9120
aaatggagca gaagtgtggg tctgcccaag gctgccccat cgaacttctc cctattagtg 9180
aaaaggctta ggagaagttg aggatcttct cgttgttctt cggaagacta ctctggagac 9240
gtctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc 9300
ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc 9360
ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc 9420
ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa 9480
gtagcacgac tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta 9540
ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgaga gcagcggccg 9600
ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc 9660
gcggtgttcc gcattctgca agcctccgga gcgcacgtcc gtctcagggg cagcttgctt 9720
gttctttttg cagaagctca gaataaacgc tcaactttgg ccgccaccat gctgaggctg 9780
ctgctggctc tgaacttgtt tccatcaatt caagtcaccg gcaacaagat ccttgtgaag 9840
cagagcccca tgctcgtggc gtacgataat gccgtgaacc tgagctgcaa atattcgtac 9900
aacctgttct cgcgcgagtt ccgggcctcg ctgcacaagg gcctggactc cgccgtggaa 9960
gtctgcgtgg tgtacgggaa ctacagccag cagctccaag tgtacagcaa gaccggattc 10020
aactgtgacg gaaagctcgg gaacgaatcc gtgactttct acctccaaaa cctttacgtg 10080
aatcagaccg acatctactt ctgcaagatt gaagtcatgt accctcctcc ctacctggag 10140
aacgagaagt ccaacggtac catcatccac gtgaagggca aacacctgtg cccgtcccct 10200
ctgttcccgg gaccgtccaa gcccttctgg gtgctcgtgg tcgtcggtgg cgtgctggcc 10260
tgttactcct tgctcgtgac tgtggcattc attatctttt gggtccggtc caagagatct 10320
cggctgctgc actccgatta catgaacatg cccccgaggc gcccgggacc gaccagaaag 10380
cattatcagc catacgcgcc ccctcgcgac ttcgccgcct accggtcatg ataaaagctt 10440
aatgctggag cctcggtagc cgttcctcct gcccgctggg cctcccaacg ggccctcctc 10500
ccctccttgc accggccctt cctggtcttt gaataaagtt tacctgactc atggtccttt 10560
cactttcaca tgggatttcc cagttatgaa attaataaaa atcagtgatt tccacatctg 10620
tgtgtgcctg tgccaggctg ggtggggaac aggaggccga gatgattccg ggaactgtca 10680
gaaggaatca atgatttcca catcctgtct gtcttatgtc ttggggggtg gggaggccag 10740
gaagattcca ggaaggtcag agtcaatcaa tggtttccac atctctcagt gcctctatct 10800
ggaggccagg tagggctggc cttgggggag ggggaggcca gaatgactcc aagagctaca 10860
ggaagggggg agggggaaaa aggccagaat aattccagga accgccaaga agatgacgtc 10920
gaggagaagt tccccaactt tcccgcctct cagcctttga aagaaagaaa ggggaggggg 10980
caggccgcgt gcagccgcga gcggtgctgg gctccggctc caattcccca tctcagtcgt 11040
tcccaaagtc ctcctgtttc atccaagcgt gtaagggtcc ccgtccttga ctccctagtg 11100
tcctgctgcc cacagtccag tcctgggaac cagcaccgat cacctcccat cgggccaatc 11160
tcagtccctt cccccctacg tcggggccca cacgctcggt gcgtgcccag ttgaaccagg 11220
cggctgcgga aaaaaaaaag cggggagaaa gtagggcccg gctactagcg gttttacggg 11280
cgcacgtagc tcaggcctca agaccttggg ctgggactgg ctgagcctgg cgggaggcgg 11340
ggtccgagtc accgcctgcc gccgcgcccc cggtttctat aaattgagcc cgcagcctcc 11400
cgcttcgctc tctgctcctc ctgttcgaca gtcagccgca tcttcttttg cgtcgccagg 11460
gggagcttgc ttgttctttt tgcagaagct cagaataaac gctcaacttt ggccgccacc 11520
<210> 228
<211> 556
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 228
Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met
1 5 10 15
Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp
20 25 30
Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln
35 40 45
Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu
50 55 60
Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile
65 70 75 80
Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu
85 90 95
Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr
100 105 110
Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp
115 120 125
Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro
130 135 140
Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala
145 150 155 160
Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Leu Pro Pro
165 170 175
Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro
180 185 190
Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser
195 200 205
Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser
210 215 220
Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp
225 230 235 240
Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala
245 250 255
Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu
260 265 270
Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly
275 280 285
Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu
290 295 300
Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg
305 310 315 320
Arg Lys Arg Lys Arg Met Thr Asp Pro Thr Arg Arg Phe Phe Lys Val
325 330 335
Thr Pro Pro Pro Gly Ser Gly Pro Gln Asn Gln Tyr Gly Asn Val Leu
340 345 350
Ser Leu Pro Thr Pro Thr Ser Gly Leu Gly Arg Ala Gln Arg Trp Ala
355 360 365
Ala Gly Leu Gly Gly Thr Ala Pro Ser Tyr Gly Asn Pro Ser Ser Asp
370 375 380
Val Gln Ala Asp Gly Ala Leu Gly Ser Arg Ser Pro Pro Gly Val Gly
385 390 395 400
Pro Glu Glu Glu Glu Gly Glu Gly Tyr Glu Glu Pro Asp Ser Glu Glu
405 410 415
Asp Ser Glu Phe Tyr Glu Asn Asp Ser Asn Leu Gly Gln Asp Gln Leu
420 425 430
Ser Gln Asp Gly Ser Gly Tyr Glu Asn Pro Glu Asp Glu Pro Leu Gly
435 440 445
Pro Glu Asp Glu Asp Ser Phe Ser Asn Ala Glu Ser Tyr Glu Asn Glu
450 455 460
Asp Glu Glu Leu Thr Gln Pro Val Ala Arg Thr Met Asp Phe Leu Ser
465 470 475 480
Pro His Gly Ser Ala Trp Asp Pro Ser Arg Glu Ala Thr Ser Leu Gly
485 490 495
Ser Gln Ser Tyr Glu Asp Met Arg Gly Ile Leu Tyr Ala Ala Pro Gln
500 505 510
Leu Arg Ser Ile Arg Gly Gln Pro Gly Pro Asn His Glu Glu Asp Ala
515 520 525
Asp Ser Tyr Glu Asn Met Asp Asn Pro Asp Gly Pro Asp Pro Ala Trp
530 535 540
Gly Gly Gly Gly Arg Met Gly Thr Trp Ser Thr Arg
545 550 555
<210> 229
<211> 486
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 229
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu
1 5 10 15
His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu
20 25 30
Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln
35 40 45
Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr
50 55 60
Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro
65 70 75 80
Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile
85 90 95
Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly
100 105 110
Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr
115 120 125
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu
130 135 140
Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser
145 150 155 160
Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly
165 170 175
Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly
180 185 190
Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser
195 200 205
Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys
210 215 220
Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys
225 230 235 240
His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly
245 250 255
Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro
260 265 270
Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu
275 280 285
Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp
290 295 300
Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly
305 310 315 320
Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg
325 330 335
Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln
340 345 350
Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu
355 360 365
Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala
370 375 380
Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu
385 390 395 400
Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp
405 410 415
Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu
420 425 430
Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile
435 440 445
Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr
450 455 460
Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met
465 470 475 480
Gln Ala Leu Pro Pro Arg
485
<210> 230
<211> 111
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 230
Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln
1 5 10 15
Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp
20 25 30
Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro
35 40 45
Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp
50 55 60
Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg
65 70 75 80
Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr
85 90 95
Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
100 105 110
<210> 231
<211> 376
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 231
Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala
1 5 10 15
Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg
20 25 30
Arg Gln Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr
35 40 45
Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr
50 55 60
Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr
65 70 75 80
Val Pro Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr
85 90 95
Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile
100 105 110
Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met
115 120 125
Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly
130 135 140
Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile
145 150 155 160
Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg
165 170 175
Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala
180 185 190
Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu
195 200 205
Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly
210 215 220
Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly
225 230 235 240
Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg
245 250 255
Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala
260 265 270
Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu
275 280 285
Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu
290 295 300
Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg
305 310 315 320
Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys
325 330 335
Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val
340 345 350
Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe
355 360 365
Ala Arg Glu Met Gly Glu Ala Asn
370 375
<210> 232
<211> 770
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 232
Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu
1 5 10 15
Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln
20 25 30
Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser
35 40 45
Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile
50 55 60
Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln
65 70 75 80
His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu
85 90 95
Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu
100 105 110
Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln
115 120 125
Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu
130 135 140
Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln
145 150 155 160
Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr
165 170 175
Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn
180 185 190
Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr
195 200 205
Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu
210 215 220
Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu
225 230 235 240
Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro
245 250 255
Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu
260 265 270
Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln
275 280 285
Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met
290 295 300
Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala
305 310 315 320
Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro
325 330 335
Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu
340 345 350
Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile
355 360 365
Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe
370 375 380
Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn
385 390 395 400
Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln
405 410 415
Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val
420 425 430
Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln
435 440 445
Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile
450 455 460
Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr
465 470 475 480
Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro
485 490 495
Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe
500 505 510
Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu
515 520 525
Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile
530 535 540
Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser
545 550 555 560
Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile
565 570 575
Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu
580 585 590
Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu
595 600 605
Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val
610 615 620
Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Tyr
625 630 635 640
Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly
645 650 655
Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr
660 665 670
Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg
675 680 685
Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro
690 695 700
Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn
705 710 715 720
Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln
725 730 735
Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe
740 745 750
Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser
755 760 765
Pro Met
770
<210> 233
<211> 220
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 233
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val
1 5 10 15
Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr
20 25 30
Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser
35 40 45
Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu
50 55 60
Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser
65 70 75 80
Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr
85 90 95
Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys
100 105 110
Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser
115 120 125
Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro
130 135 140
Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly
145 150 155 160
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile
165 170 175
Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met
180 185 190
Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro
195 200 205
Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser
210 215 220
<210> 234
<211> 193
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 234
Met Ala Ala Ile Arg Lys Lys Leu Val Ile Val Gly Asp Gly Ala Cys
1 5 10 15
Gly Lys Thr Cys Leu Leu Ile Val Phe Ser Lys Asp Gln Phe Pro Glu
20 25 30
Val Tyr Val Pro Thr Val Phe Glu Asn Tyr Val Ala Asp Ile Glu Val
35 40 45
Asp Gly Lys Gln Val Glu Leu Ala Leu Trp Asp Thr Ala Gly Gln Glu
50 55 60
Asp Tyr Asp Arg Leu Arg Pro Leu Ser Tyr Pro Asp Thr Asp Val Ile
65 70 75 80
Leu Met Cys Phe Ser Ile Asp Ser Pro Asp Ser Leu Glu Asn Ile Pro
85 90 95
Glu Lys Trp Thr Pro Glu Val Lys His Phe Cys Pro Asn Val Pro Ile
100 105 110
Ile Leu Val Gly Asn Lys Lys Asp Leu Arg Asn Asp Glu His Thr Arg
115 120 125
Arg Glu Leu Ala Lys Met Lys Gln Glu Pro Val Lys Pro Glu Glu Gly
130 135 140
Arg Asp Met Ala Asn Arg Ile Gly Ala Phe Gly Tyr Met Glu Cys Ser
145 150 155 160
Ala Lys Thr Lys Asp Gly Val Arg Glu Val Phe Glu Met Ala Thr Arg
165 170 175
Ala Ala Leu Gln Ala Arg Arg Gly Lys Lys Lys Ser Gly Cys Leu Val
180 185 190
Leu
<210> 235
<211> 1290
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 235
Met Ala Gly Ala Ala Ser Pro Cys Ala Asn Gly Cys Gly Pro Gly Ala
1 5 10 15
Pro Ser Asp Ala Glu Val Leu His Leu Cys Arg Ser Leu Glu Val Gly
20 25 30
Thr Val Met Thr Leu Phe Tyr Ser Lys Lys Ser Gln Arg Pro Glu Arg
35 40 45
Lys Thr Phe Gln Val Lys Leu Glu Thr Arg Gln Ile Thr Trp Ser Arg
50 55 60
Gly Ala Asp Lys Ile Glu Gly Ala Ile Asp Ile Arg Glu Ile Lys Glu
65 70 75 80
Ile Arg Pro Gly Lys Thr Ser Arg Asp Phe Asp Arg Tyr Gln Glu Asp
85 90 95
Pro Ala Phe Arg Pro Asp Gln Ser His Cys Phe Val Ile Leu Tyr Gly
100 105 110
Met Glu Phe Arg Leu Lys Thr Leu Ser Leu Gln Ala Thr Ser Glu Asp
115 120 125
Glu Val Asn Met Trp Ile Lys Gly Leu Thr Trp Leu Met Glu Asp Thr
130 135 140
Leu Gln Ala Pro Thr Pro Leu Gln Ile Glu Arg Trp Leu Arg Lys Gln
145 150 155 160
Phe Tyr Ser Val Asp Arg Asn Arg Glu Asp Arg Ile Ser Ala Lys Asp
165 170 175
Leu Lys Asn Met Leu Ser Gln Val Asn Tyr Arg Val Pro Asn Met Arg
180 185 190
Phe Leu Arg Glu Arg Leu Thr Asp Leu Glu Gln Arg Ser Gly Asp Ile
195 200 205
Thr Tyr Gly Gln Phe Ala Gln Leu Tyr Arg Ser Leu Met Tyr Ser Ala
210 215 220
Gln Lys Thr Met Asp Leu Pro Phe Leu Glu Ala Ser Thr Leu Arg Ala
225 230 235 240
Gly Glu Arg Pro Glu Leu Cys Arg Val Ser Leu Pro Glu Phe Gln Gln
245 250 255
Phe Leu Leu Asp Tyr Gln Gly Glu Leu Trp Ala Val Asp Arg Leu Gln
260 265 270
Val Gln Glu Phe Met Leu Ser Phe Leu Arg Asp Pro Leu Arg Glu Ile
275 280 285
Glu Glu Pro Tyr Phe Phe Leu Asp Glu Phe Val Thr Phe Leu Phe Ser
290 295 300
Lys Glu Asn Ser Val Trp Asn Ser Gln Leu Asp Ala Val Cys Pro Asp
305 310 315 320
Thr Met Asn Asn Pro Leu Ser His Tyr Trp Ile Ser Ser Ser His Asn
325 330 335
Thr Tyr Leu Thr Gly Asp Gln Phe Ser Ser Glu Ser Ser Leu Glu Ala
340 345 350
Tyr Ala Arg Cys Leu Arg Met Gly Cys Arg Cys Ile Glu Leu Asp Cys
355 360 365
Trp Asp Gly Pro Asp Gly Met Pro Val Ile Tyr His Gly His Thr Leu
370 375 380
Thr Thr Lys Ile Lys Phe Ser Asp Val Leu His Thr Ile Lys Glu His
385 390 395 400
Ala Phe Val Ala Ser Glu Tyr Pro Val Ile Leu Ser Ile Glu Asp His
405 410 415
Cys Ser Ile Ala Gln Gln Arg Asn Met Ala Gln Tyr Phe Lys Lys Val
420 425 430
Leu Gly Asp Thr Leu Leu Thr Lys Pro Val Glu Ile Ser Ala Asp Gly
435 440 445
Leu Pro Ser Pro Asn Gln Leu Lys Arg Lys Ile Leu Ile Lys His Lys
450 455 460
Lys Leu Ala Glu Gly Ser Ala Tyr Glu Glu Val Pro Thr Ser Met Met
465 470 475 480
Tyr Ser Glu Asn Asp Ile Ser Asn Ser Ile Lys Asn Gly Ile Leu Tyr
485 490 495
Leu Glu Asp Pro Val Asn His Glu Trp Tyr Pro His Tyr Phe Val Leu
500 505 510
Thr Ser Ser Lys Ile Tyr Tyr Ser Glu Glu Thr Ser Ser Asp Gln Gly
515 520 525
Asn Glu Asp Glu Glu Glu Pro Lys Glu Val Ser Ser Ser Thr Glu Leu
530 535 540
His Ser Asn Glu Lys Trp Phe His Gly Lys Leu Gly Ala Gly Arg Asp
545 550 555 560
Gly Arg His Ile Ala Glu Arg Leu Leu Thr Glu Tyr Cys Ile Glu Thr
565 570 575
Gly Ala Pro Asp Gly Ser Phe Leu Val Arg Glu Ser Glu Thr Phe Val
580 585 590
Gly Asp Tyr Thr Leu Ser Phe Trp Arg Asn Gly Lys Val Gln His Cys
595 600 605
Arg Ile His Ser Arg Gln Asp Ala Gly Thr Pro Lys Phe Phe Leu Thr
610 615 620
Asp Asn Leu Val Phe Asp Ser Leu Tyr Asp Leu Ile Thr His Tyr Gln
625 630 635 640
Gln Val Pro Leu Arg Cys Asn Glu Phe Glu Met Arg Leu Ser Glu Pro
645 650 655
Val Pro Gln Thr Asn Ala His Glu Ser Lys Glu Trp Tyr His Ala Ser
660 665 670
Leu Thr Arg Ala Gln Ala Glu His Met Leu Met Arg Val Pro Arg Asp
675 680 685
Gly Ala Phe Leu Val Arg Lys Arg Asn Glu Pro Asn Ser Tyr Ala Ile
690 695 700
Ser Phe Arg Ala Glu Gly Lys Ile Lys His Cys Arg Val Gln Gln Glu
705 710 715 720
Gly Gln Thr Val Met Leu Gly Asn Ser Glu Phe Asp Ser Leu Val Asp
725 730 735
Leu Ile Ser Tyr Tyr Glu Lys His Pro Leu Tyr Arg Lys Met Lys Leu
740 745 750
Arg Tyr Pro Ile Asn Glu Glu Ala Leu Glu Lys Ile Gly Thr Ala Glu
755 760 765
Pro Asp Tyr Gly Ala Leu Tyr Glu Gly Arg Asn Pro Gly Phe Tyr Val
770 775 780
Glu Ala Asn Pro Met Pro Thr Phe Lys Cys Ala Val Lys Ala Leu Phe
785 790 795 800
Asp Tyr Lys Ala Gln Arg Glu Asp Glu Leu Thr Phe Ile Lys Ser Ala
805 810 815
Ile Ile Gln Asn Val Glu Lys Gln Glu Gly Gly Trp Trp Arg Gly Asp
820 825 830
Tyr Gly Gly Lys Lys Gln Leu Trp Phe Pro Ser Asn Tyr Val Glu Glu
835 840 845
Met Val Asn Pro Val Ala Leu Glu Pro Glu Arg Glu His Leu Asp Glu
850 855 860
Asn Ser Pro Leu Gly Asp Leu Leu Arg Gly Val Leu Asp Val Pro Ala
865 870 875 880
Cys Gln Ile Ala Ile Arg Pro Glu Gly Lys Asn Asn Arg Leu Phe Val
885 890 895
Phe Ser Ile Ser Met Ala Ser Val Ala His Trp Ser Leu Asp Val Ala
900 905 910
Ala Asp Ser Gln Glu Glu Leu Gln Asp Trp Val Lys Lys Ile Arg Glu
915 920 925
Val Ala Gln Thr Ala Asp Ala Arg Leu Thr Glu Gly Lys Ile Met Glu
930 935 940
Arg Arg Lys Lys Ile Ala Leu Glu Leu Ser Glu Leu Val Val Tyr Cys
945 950 955 960
Arg Pro Val Pro Phe Asp Glu Glu Lys Ile Gly Thr Glu Arg Ala Cys
965 970 975
Tyr Arg Asp Met Ser Ser Phe Pro Glu Thr Lys Ala Glu Lys Tyr Val
980 985 990
Asn Lys Ala Lys Gly Lys Lys Phe Leu Gln Tyr Asn Arg Leu Gln Leu
995 1000 1005
Ser Arg Ile Tyr Pro Lys Gly Gln Arg Leu Asp Ser Ser Asn Tyr
1010 1015 1020
Asp Pro Leu Pro Met Trp Ile Cys Gly Ser Gln Leu Val Ala Leu
1025 1030 1035
Asn Phe Gln Thr Pro Asp Lys Pro Met Gln Met Asn Gln Ala Leu
1040 1045 1050
Phe Met Thr Gly Arg His Cys Gly Tyr Val Leu Gln Pro Ser Thr
1055 1060 1065
Met Arg Asp Glu Ala Phe Asp Pro Phe Asp Lys Ser Ser Leu Arg
1070 1075 1080
Gly Leu Glu Pro Cys Ala Ile Ser Ile Glu Val Leu Gly Ala Arg
1085 1090 1095
His Leu Pro Lys Asn Gly Arg Gly Ile Val Cys Pro Phe Val Glu
1100 1105 1110
Ile Glu Val Ala Gly Ala Glu Tyr Asp Ser Thr Lys Gln Lys Thr
1115 1120 1125
Glu Phe Val Val Asp Asn Gly Leu Asn Pro Val Trp Pro Ala Lys
1130 1135 1140
Pro Phe His Phe Gln Ile Ser Asn Pro Glu Phe Ala Phe Leu Arg
1145 1150 1155
Phe Val Val Tyr Glu Glu Asp Met Phe Ser Asp Gln Asn Phe Leu
1160 1165 1170
Ala Gln Ala Thr Phe Pro Val Lys Gly Leu Lys Thr Gly Tyr Arg
1175 1180 1185
Ala Val Pro Leu Lys Asn Asn Tyr Ser Glu Asp Leu Glu Leu Ala
1190 1195 1200
Ser Leu Leu Ile Lys Ile Asp Ile Phe Pro Ala Lys Glu Asn Gly
1205 1210 1215
Asp Leu Ser Pro Phe Ser Gly Thr Ser Leu Arg Glu Arg Gly Ser
1220 1225 1230
Asp Ala Ser Gly Gln Leu Phe His Gly Arg Ala Arg Glu Gly Ser
1235 1240 1245
Phe Glu Ser Arg Tyr Gln Gln Pro Phe Glu Asp Phe Arg Ile Ser
1250 1255 1260
Gln Glu His Leu Ala Asp His Phe Asp Ser Arg Glu Arg Arg Ala
1265 1270 1275
Pro Arg Arg Thr Arg Val Asn Gly Asp Asn Arg Leu
1280 1285 1290
<210> 236
<211> 787
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 236
Met Ala Val Trp Ile Gln Ala Gln Gln Leu Gln Gly Glu Ala Leu His
1 5 10 15
Gln Met Gln Ala Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His
20 25 30
Tyr Leu Ser Gln Trp Ile Glu Ser Gln Ala Trp Asp Ser Val Asp Leu
35 40 45
Asp Asn Pro Gln Glu Asn Ile Lys Ala Thr Gln Leu Leu Glu Gly Leu
50 55 60
Val Gln Glu Leu Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly
65 70 75 80
Phe Leu Leu Lys Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Asn
85 90 95
Thr Tyr Asp Arg Cys Pro Met Glu Leu Val Arg Cys Ile Arg His Ile
100 105 110
Leu Tyr Asn Glu Gln Arg Leu Val Arg Glu Ala Asn Asn Gly Ser Ser
115 120 125
Pro Ala Gly Ser Leu Ala Asp Ala Met Ser Gln Lys His Leu Gln Ile
130 135 140
Asn Gln Thr Phe Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn
145 150 155 160
Glu Leu Lys Lys Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr
165 170 175
Gln Glu Ser Leu Arg Ile Gln Ala Gln Phe Gly Pro Leu Ala Gln Leu
180 185 190
Ser Pro Gln Glu Arg Leu Ser Arg Glu Thr Ala Leu Gln Gln Lys Gln
195 200 205
Val Ser Leu Glu Ala Trp Leu Gln Arg Glu Ala Gln Thr Leu Gln Gln
210 215 220
Tyr Arg Val Glu Leu Ala Glu Lys His Gln Lys Thr Leu Gln Leu Leu
225 230 235 240
Arg Lys Gln Gln Thr Ile Ile Leu Asp Asp Glu Leu Ile Gln Trp Lys
245 250 255
Arg Arg Gln Gln Leu Ala Gly Asn Gly Gly Pro Pro Glu Gly Ser Leu
260 265 270
Asp Val Leu Gln Ser Trp Cys Glu Lys Leu Ala Glu Ile Ile Trp Gln
275 280 285
Asn Arg Gln Gln Ile Arg Arg Ala Glu His Leu Cys Gln Gln Leu Pro
290 295 300
Ile Pro Gly Pro Val Glu Glu Met Leu Ala Glu Val Asn Ala Thr Ile
305 310 315 320
Thr Asp Ile Ile Ser Ala Leu Val Thr Ser Thr Phe Ile Ile Glu Lys
325 330 335
Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys Phe Ala Ala Thr Val
340 345 350
Arg Leu Leu Val Gly Gly Lys Leu Asn Val His Met Asn Pro Pro Gln
355 360 365
Val Lys Ala Thr Ile Ile Ser Glu Gln Gln Ala Lys Ser Leu Leu Lys
370 375 380
Asn Glu Asn Thr Arg Asn Asp Tyr Ser Gly Glu Ile Leu Asn Asn Cys
385 390 395 400
Cys Val Met Glu Tyr His Gln Ala Thr Gly Thr Leu Ser Ala His Phe
405 410 415
Arg Asn Met Ser Leu Lys Arg Ile Lys Arg Ser Asp Arg Arg Gly Ala
420 425 430
Glu Ser Val Thr Glu Glu Lys Phe Thr Ile Leu Phe Glu Ser Gln Phe
435 440 445
Ser Val Gly Gly Asn Glu Leu Val Phe Gln Val Lys Thr Leu Ser Leu
450 455 460
Pro Val Val Val Ile Val His Gly Ser Gln Asp Asn Asn Ala Thr Ala
465 470 475 480
Thr Val Leu Trp Asp Asn Ala Phe Ala Glu Pro Gly Arg Val Pro Phe
485 490 495
Ala Val Pro Asp Lys Val Leu Trp Pro Gln Leu Cys Glu Ala Leu Asn
500 505 510
Met Lys Phe Lys Ala Glu Val Gln Ser Asn Arg Gly Leu Thr Lys Glu
515 520 525
Asn Leu Val Phe Leu Ala Gln Lys Leu Phe Asn Asn Ser Ser Ser His
530 535 540
Leu Glu Asp Tyr Ser Gly Leu Ser Val Ser Trp Ser Gln Phe Asn Arg
545 550 555 560
Glu Asn Leu Pro Gly Arg Asn Tyr Thr Phe Trp Gln Trp Phe Asp Gly
565 570 575
Val Met Glu Val Leu Lys Lys His Leu Lys Pro His Trp Asn Asp Gly
580 585 590
Ala Ile Leu Gly Phe Val Asn Lys Gln Gln Ala His Asp Leu Leu Ile
595 600 605
Asn Lys Pro Asp Gly Thr Phe Leu Leu Arg Phe Ser Asp Ser Glu Ile
610 615 620
Gly Gly Ile Thr Ile Ala Trp Lys Phe Asp Ser Gln Glu Arg Met Phe
625 630 635 640
Trp Asn Leu Met Pro Phe Thr Thr Arg Asp Phe Ser Ile Arg Ser Leu
645 650 655
Ala Asp Arg Leu Gly Asp Leu Asn Tyr Leu Ile Tyr Val Phe Pro Asp
660 665 670
Arg Pro Lys Asp Glu Val Tyr Ser Lys Tyr Tyr Thr Pro Val Pro Cys
675 680 685
Glu Ser Ala Thr Ala Lys Ala Val Asp Gly Tyr Val Lys Pro Gln Ile
690 695 700
Lys Gln Val Val Pro Glu Phe Val Asn Ala Ser Ala Asp Ala Gly Gly
705 710 715 720
Gly Ser Ala Thr Tyr Met Asp Gln Ala Pro Ser Pro Ala Val Cys Pro
725 730 735
Gln Ala His Tyr Asn Met Tyr Pro Gln Asn Pro Asp Ser Val Leu Asp
740 745 750
Thr Asp Gly Asp Phe Asp Leu Glu Asp Thr Met Asp Val Ala Arg Arg
755 760 765
Val Glu Glu Leu Leu Gly Arg Pro Met Asp Ser Gln Trp Ile Pro His
770 775 780
Ala Gln Ser
785
<210> 237
<211> 142
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 237
Met Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp
1 5 10 15
His Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala
20 25 30
Cys Thr Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr
35 40 45
Glu Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu
50 55 60
Glu Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His
65 70 75 80
Ser Ser Gly Cys Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu
85 90 95
Thr Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys
100 105 110
Ile Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala
115 120 125
Lys Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp
130 135 140
<210> 238
<211> 233
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 238
Met Ser Gln Ser Asn Arg Glu Leu Val Val Asp Phe Leu Ser Tyr Lys
1 5 10 15
Leu Ser Gln Lys Gly Tyr Ser Trp Ser Gln Phe Ser Asp Val Glu Glu
20 25 30
Asn Arg Thr Glu Ala Pro Glu Gly Thr Glu Ser Glu Met Glu Thr Pro
35 40 45
Ser Ala Ile Asn Gly Asn Pro Ser Trp His Leu Ala Asp Ser Pro Ala
50 55 60
Val Asn Gly Ala Thr Gly His Ser Ser Ser Leu Asp Ala Arg Glu Val
65 70 75 80
Ile Pro Met Ala Ala Val Lys Gln Ala Leu Arg Glu Ala Gly Asp Glu
85 90 95
Phe Glu Leu Arg Tyr Arg Arg Ala Phe Ser Asp Leu Thr Ser Gln Leu
100 105 110
His Ile Thr Pro Gly Thr Ala Tyr Gln Ser Phe Glu Gln Val Val Asn
115 120 125
Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile Val Ala Phe Phe
130 135 140
Ser Phe Gly Gly Ala Leu Cys Val Glu Ser Val Asp Lys Glu Met Gln
145 150 155 160
Val Leu Val Ser Arg Ile Ala Ala Trp Met Ala Thr Tyr Leu Asn Asp
165 170 175
His Leu Glu Pro Trp Ile Gln Glu Asn Gly Gly Trp Asp Thr Phe Val
180 185 190
Glu Leu Tyr Gly Asn Asn Ala Ala Ala Glu Ser Arg Lys Gly Gln Glu
195 200 205
Arg Phe Asn Arg Trp Phe Leu Thr Gly Met Thr Val Ala Gly Val Val
210 215 220
Leu Leu Gly Ser Leu Phe Ser Arg Lys
225 230
<210> 239
<211> 295
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 239
Met Glu His Gln Leu Leu Cys Cys Glu Val Glu Thr Ile Arg Arg Ala
1 5 10 15
Tyr Pro Asp Ala Asn Leu Leu Asn Asp Arg Val Leu Arg Ala Met Leu
20 25 30
Lys Ala Glu Glu Thr Cys Ala Pro Ser Val Ser Tyr Phe Lys Cys Val
35 40 45
Gln Lys Glu Val Leu Pro Ser Met Arg Lys Ile Val Ala Thr Trp Met
50 55 60
Leu Glu Val Cys Glu Glu Gln Lys Cys Glu Glu Glu Val Phe Pro Leu
65 70 75 80
Ala Met Asn Tyr Leu Asp Arg Phe Leu Ser Leu Glu Pro Val Lys Lys
85 90 95
Ser Arg Leu Gln Leu Leu Gly Ala Thr Cys Met Phe Val Ala Ser Lys
100 105 110
Met Lys Glu Thr Ile Pro Leu Thr Ala Glu Lys Leu Cys Ile Tyr Thr
115 120 125
Asp Asn Ser Ile Arg Pro Glu Glu Leu Leu Gln Met Glu Leu Leu Leu
130 135 140
Val Asn Lys Leu Lys Trp Asn Leu Ala Ala Met Thr Pro His Asp Phe
145 150 155 160
Ile Glu His Phe Leu Ser Lys Met Pro Glu Ala Glu Glu Asn Lys Gln
165 170 175
Ile Ile Arg Lys His Ala Gln Thr Phe Val Ala Leu Cys Ala Thr Asp
180 185 190
Val Lys Phe Ile Ser Asn Pro Pro Ser Met Val Ala Ala Gly Ser Val
195 200 205
Val Ala Ala Val Gln Gly Leu Asn Leu Arg Ser Pro Asn Asn Phe Leu
210 215 220
Ser Tyr Tyr Arg Leu Thr Arg Phe Leu Ser Arg Val Ile Lys Cys Asp
225 230 235 240
Pro Asp Cys Leu Arg Ala Cys Gln Glu Gln Ile Glu Ala Leu Leu Glu
245 250 255
Ser Ser Leu Arg Gln Ala Gln Gln Asn Met Asp Pro Lys Ala Ala Glu
260 265 270
Glu Glu Glu Glu Glu Glu Glu Glu Val Asp Leu Ala Cys Thr Pro Thr
275 280 285
Asp Val Arg Asp Val Asp Ile
290 295
<210> 240
<211> 277
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 240
Met Glu Asn Thr Glu Asn Ser Val Asp Ser Lys Ser Ile Lys Asn Leu
1 5 10 15
Glu Pro Lys Ile Ile His Gly Ser Glu Ser Met Asp Ser Gly Ile Ser
20 25 30
Leu Asp Asn Ser Tyr Lys Met Asp Tyr Pro Glu Met Gly Leu Cys Ile
35 40 45
Ile Ile Asn Asn Lys Asn Phe His Lys Ser Thr Gly Met Thr Ser Arg
50 55 60
Ser Gly Thr Asp Val Asp Ala Ala Asn Leu Arg Glu Thr Phe Arg Asn
65 70 75 80
Leu Lys Tyr Glu Val Arg Asn Lys Asn Asp Leu Thr Arg Glu Glu Ile
85 90 95
Val Glu Leu Met Arg Asp Val Ser Lys Glu Asp His Ser Lys Arg Ser
100 105 110
Ser Phe Val Cys Val Leu Leu Ser His Gly Glu Glu Gly Ile Ile Phe
115 120 125
Gly Thr Asn Gly Pro Val Asp Leu Lys Lys Ile Thr Asn Phe Phe Arg
130 135 140
Gly Asp Arg Cys Arg Ser Leu Thr Gly Lys Pro Lys Leu Phe Ile Ile
145 150 155 160
Gln Ala Cys Arg Gly Thr Glu Leu Asp Cys Gly Ile Glu Thr Asp Ser
165 170 175
Gly Val Asp Asp Asp Met Ala Cys His Lys Ile Pro Val Glu Ala Asp
180 185 190
Phe Leu Tyr Ala Tyr Ser Thr Ala Pro Gly Tyr Tyr Ser Trp Arg Asn
195 200 205
Ser Lys Asp Gly Ser Trp Phe Ile Gln Ser Leu Cys Ala Met Leu Lys
210 215 220
Gln Tyr Ala Asp Lys Leu Glu Phe Met His Ile Leu Thr Arg Val Asn
225 230 235 240
Arg Lys Val Ala Thr Glu Phe Glu Ser Phe Ser Phe Asp Ala Thr Phe
245 250 255
His Ala Lys Lys Gln Ile Pro Cys Ile Val Ser Met Leu Thr Lys Glu
260 265 270
Leu Tyr Phe Tyr His
275
<210> 241
<211> 303
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 241
Met Ala Asp Asp Gln Gly Cys Ile Glu Glu Gln Gly Val Glu Asp Ser
1 5 10 15
Ala Asn Glu Asp Ser Val Asp Ala Lys Pro Asp Arg Ser Ser Phe Val
20 25 30
Pro Ser Leu Phe Ser Lys Lys Lys Lys Asn Val Thr Met Arg Ser Ile
35 40 45
Lys Thr Thr Arg Asp Arg Val Pro Thr Tyr Gln Tyr Asn Met Asn Phe
50 55 60
Glu Lys Leu Gly Lys Cys Ile Ile Ile Asn Asn Lys Asn Phe Asp Lys
65 70 75 80
Val Thr Gly Met Gly Val Arg Asn Gly Thr Asp Lys Asp Ala Glu Ala
85 90 95
Leu Phe Lys Cys Phe Arg Ser Leu Gly Phe Asp Val Ile Val Tyr Asn
100 105 110
Asp Cys Ser Cys Ala Lys Met Gln Asp Leu Leu Lys Lys Ala Ser Glu
115 120 125
Glu Asp His Thr Asn Ala Ala Cys Phe Ala Cys Ile Leu Leu Ser His
130 135 140
Gly Glu Glu Asn Val Ile Tyr Gly Lys Asp Gly Val Thr Pro Ile Lys
145 150 155 160
Asp Leu Thr Ala His Phe Arg Gly Asp Arg Cys Lys Thr Leu Leu Glu
165 170 175
Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Arg Gly Thr Glu Leu Asp
180 185 190
Asp Gly Ile Gln Ala Asp Ser Gly Pro Ile Asn Asp Thr Asp Ala Asn
195 200 205
Pro Arg Tyr Lys Ile Pro Val Glu Ala Asp Phe Leu Phe Ala Tyr Ser
210 215 220
Thr Val Pro Gly Tyr Tyr Ser Trp Arg Ser Pro Gly Arg Gly Ser Trp
225 230 235 240
Phe Val Gln Ala Leu Cys Ser Ile Leu Glu Glu His Gly Lys Asp Leu
245 250 255
Glu Ile Met Gln Ile Leu Thr Arg Val Asn Asp Arg Val Ala Arg His
260 265 270
Phe Glu Ser Gln Ser Asp Asp Pro His Phe His Glu Lys Lys Gln Ile
275 280 285
Pro Cys Val Val Ser Met Leu Thr Lys Glu Leu Tyr Phe Ser Gln
290 295 300
<210> 242
<211> 479
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 242
Met Asp Phe Ser Arg Asn Leu Tyr Asp Ile Gly Glu Gln Leu Asp Ser
1 5 10 15
Glu Asp Leu Ala Ser Leu Lys Phe Leu Ser Leu Asp Tyr Ile Pro Gln
20 25 30
Arg Lys Gln Glu Pro Ile Lys Asp Ala Leu Met Leu Phe Gln Arg Leu
35 40 45
Gln Glu Lys Arg Met Leu Glu Glu Ser Asn Leu Ser Phe Leu Lys Glu
50 55 60
Leu Leu Phe Arg Ile Asn Arg Leu Asp Leu Leu Ile Thr Tyr Leu Asn
65 70 75 80
Thr Arg Lys Glu Glu Met Glu Arg Glu Leu Gln Thr Pro Gly Arg Ala
85 90 95
Gln Ile Ser Ala Tyr Arg Val Met Leu Tyr Gln Ile Ser Glu Glu Val
100 105 110
Ser Arg Ser Glu Leu Arg Ser Phe Lys Phe Leu Leu Gln Glu Glu Ile
115 120 125
Ser Lys Cys Lys Leu Asp Asp Asp Met Asn Leu Leu Asp Ile Phe Ile
130 135 140
Glu Met Glu Lys Arg Val Ile Leu Gly Glu Gly Lys Leu Asp Ile Leu
145 150 155 160
Lys Arg Val Cys Ala Gln Ile Asn Lys Ser Leu Leu Lys Ile Ile Asn
165 170 175
Asp Tyr Glu Glu Phe Ser Lys Glu Arg Ser Ser Ser Leu Glu Gly Ser
180 185 190
Pro Asp Glu Phe Ser Asn Gly Glu Glu Leu Cys Gly Val Met Thr Ile
195 200 205
Ser Asp Ser Pro Arg Glu Gln Asp Ser Glu Ser Gln Thr Leu Asp Lys
210 215 220
Val Tyr Gln Met Lys Ser Lys Pro Arg Gly Tyr Cys Leu Ile Ile Asn
225 230 235 240
Asn His Asn Phe Ala Lys Ala Arg Glu Lys Val Pro Lys Leu His Ser
245 250 255
Ile Arg Asp Arg Asn Gly Thr His Leu Asp Ala Gly Ala Leu Thr Thr
260 265 270
Thr Phe Glu Glu Leu His Phe Glu Ile Lys Pro His Asp Asp Cys Thr
275 280 285
Val Glu Gln Ile Tyr Glu Ile Leu Lys Ile Tyr Gln Leu Met Asp His
290 295 300
Ser Asn Met Asp Cys Phe Ile Cys Cys Ile Leu Ser His Gly Asp Lys
305 310 315 320
Gly Ile Ile Tyr Gly Thr Asp Gly Gln Glu Ala Pro Ile Tyr Glu Leu
325 330 335
Thr Ser Gln Phe Thr Gly Leu Lys Cys Pro Ser Leu Ala Gly Lys Pro
340 345 350
Lys Val Phe Phe Ile Gln Ala Cys Gln Gly Asp Asn Tyr Gln Lys Gly
355 360 365
Ile Pro Val Glu Thr Asp Ser Glu Glu Gln Pro Tyr Leu Glu Met Asp
370 375 380
Leu Ser Ser Pro Gln Thr Arg Tyr Ile Pro Asp Glu Ala Asp Phe Leu
385 390 395 400
Leu Gly Met Ala Thr Val Asn Asn Cys Val Ser Tyr Arg Asn Pro Ala
405 410 415
Glu Gly Thr Trp Tyr Ile Gln Ser Leu Cys Gln Ser Leu Arg Glu Arg
420 425 430
Cys Pro Arg Gly Asp Asp Ile Leu Thr Ile Leu Thr Glu Val Asn Tyr
435 440 445
Glu Val Ser Asn Lys Asp Asp Lys Lys Asn Met Gly Lys Gln Met Pro
450 455 460
Gln Pro Thr Phe Thr Leu Arg Lys Lys Leu Val Phe Pro Ser Asp
465 470 475
<210> 243
<211> 416
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 243
Met Asp Glu Ala Asp Arg Arg Leu Leu Arg Arg Cys Arg Leu Arg Leu
1 5 10 15
Val Glu Glu Leu Gln Val Asp Gln Leu Trp Asp Ala Leu Leu Ser Arg
20 25 30
Glu Leu Phe Arg Pro His Met Ile Glu Asp Ile Gln Arg Ala Gly Ser
35 40 45
Gly Ser Arg Arg Asp Gln Ala Arg Gln Leu Ile Ile Asp Leu Glu Thr
50 55 60
Arg Gly Ser Gln Ala Leu Pro Leu Phe Ile Ser Cys Leu Glu Asp Thr
65 70 75 80
Gly Gln Asp Met Leu Ala Ser Phe Leu Arg Thr Asn Arg Gln Ala Ala
85 90 95
Lys Leu Ser Lys Pro Thr Leu Glu Asn Leu Thr Pro Val Val Leu Arg
100 105 110
Pro Glu Ile Arg Lys Pro Glu Val Leu Arg Pro Glu Thr Pro Arg Pro
115 120 125
Val Asp Ile Gly Ser Gly Gly Phe Gly Asp Val Gly Ala Leu Glu Ser
130 135 140
Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys
145 150 155 160
Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly
165 170 175
Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg
180 185 190
Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr
195 200 205
Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Gln Gln Asp His
210 215 220
Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln
225 230 235 240
Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys
245 250 255
Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys
260 265 270
Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly
275 280 285
Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu
290 295 300
Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln
305 310 315 320
Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro
325 330 335
Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val
340 345 350
Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp
355 360 365
Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu
370 375 380
Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met
385 390 395 400
Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser
405 410 415
<210> 244
<211> 521
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 244
Met Lys Ser Gln Gly Gln His Trp Tyr Ser Ser Ser Asp Lys Asn Cys
1 5 10 15
Lys Val Ser Phe Arg Glu Lys Leu Leu Ile Ile Asp Ser Asn Leu Gly
20 25 30
Val Gln Asp Val Glu Asn Leu Lys Phe Leu Cys Ile Gly Leu Val Pro
35 40 45
Asn Lys Lys Leu Glu Lys Ser Ser Ser Ala Ser Asp Val Phe Glu His
50 55 60
Leu Leu Ala Glu Asp Leu Leu Ser Glu Glu Asp Pro Phe Phe Leu Ala
65 70 75 80
Glu Leu Leu Tyr Ile Ile Arg Gln Lys Lys Leu Leu Gln His Leu Asn
85 90 95
Cys Thr Lys Glu Glu Val Glu Arg Leu Leu Pro Thr Arg Gln Arg Val
100 105 110
Ser Leu Phe Arg Asn Leu Leu Tyr Glu Leu Ser Glu Gly Ile Asp Ser
115 120 125
Glu Asn Leu Lys Asp Met Ile Phe Leu Leu Lys Asp Ser Leu Pro Lys
130 135 140
Thr Glu Met Thr Ser Leu Ser Phe Leu Ala Phe Leu Glu Lys Gln Gly
145 150 155 160
Lys Ile Asp Glu Asp Asn Leu Thr Cys Leu Glu Asp Leu Cys Lys Thr
165 170 175
Val Val Pro Lys Leu Leu Arg Asn Ile Glu Lys Tyr Lys Arg Glu Lys
180 185 190
Ala Ile Gln Ile Val Thr Pro Pro Val Asp Lys Glu Ala Glu Ser Tyr
195 200 205
Gln Gly Glu Glu Glu Leu Val Ser Gln Thr Asp Val Lys Thr Phe Leu
210 215 220
Glu Ala Leu Pro Gln Glu Ser Trp Gln Asn Lys His Ala Gly Ser Asn
225 230 235 240
Gly Asn Arg Ala Thr Asn Gly Ala Pro Ser Leu Val Ser Arg Gly Met
245 250 255
Gln Gly Ala Ser Ala Asn Thr Leu Asn Ser Glu Thr Ser Thr Lys Arg
260 265 270
Ala Ala Val Tyr Arg Met Asn Arg Asn His Arg Gly Leu Cys Val Ile
275 280 285
Val Asn Asn His Ser Phe Thr Ser Leu Lys Asp Arg Gln Gly Thr His
290 295 300
Lys Asp Ala Glu Ile Leu Ser His Val Phe Gln Trp Leu Gly Phe Thr
305 310 315 320
Val His Ile His Asn Asn Val Thr Lys Val Glu Met Glu Met Val Leu
325 330 335
Gln Lys Gln Lys Cys Asn Pro Ala His Ala Asp Gly Asp Cys Phe Val
340 345 350
Phe Cys Ile Leu Thr His Gly Arg Phe Gly Ala Val Tyr Ser Ser Asp
355 360 365
Glu Ala Leu Ile Pro Ile Arg Glu Ile Met Ser His Phe Thr Ala Leu
370 375 380
Gln Cys Pro Arg Leu Ala Glu Lys Pro Lys Leu Phe Phe Ile Gln Ala
385 390 395 400
Cys Gln Gly Glu Glu Ile Gln Pro Ser Val Ser Ile Glu Ala Asp Ala
405 410 415
Leu Asn Pro Glu Gln Ala Pro Thr Ser Leu Gln Asp Ser Ile Pro Ala
420 425 430
Glu Ala Asp Phe Leu Leu Gly Leu Ala Thr Val Pro Gly Tyr Val Ser
435 440 445
Phe Arg His Val Glu Glu Gly Ser Trp Tyr Ile Gln Ser Leu Cys Asn
450 455 460
His Leu Lys Lys Leu Val Pro Arg Met Leu Lys Phe Leu Glu Lys Thr
465 470 475 480
Met Glu Ile Arg Gly Arg Lys Arg Thr Val Trp Gly Ala Lys Gln Ile
485 490 495
Ser Ala Thr Ser Leu Pro Thr Ala Ile Ser Ala Gln Thr Pro Arg Pro
500 505 510
Pro Met Arg Arg Trp Ser Ser Val Ser
515 520
<210> 245
<211> 480
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 245
Met Ser Ala Glu Val Ile His Gln Val Glu Glu Ala Leu Asp Thr Asp
1 5 10 15
Glu Lys Glu Met Leu Leu Phe Leu Cys Arg Asp Val Ala Ile Asp Val
20 25 30
Val Pro Pro Asn Val Arg Asp Leu Leu Asp Ile Leu Arg Glu Arg Gly
35 40 45
Lys Leu Ser Val Gly Asp Leu Ala Glu Leu Leu Tyr Arg Val Arg Arg
50 55 60
Phe Asp Leu Leu Lys Arg Ile Leu Lys Met Asp Arg Lys Ala Val Glu
65 70 75 80
Thr His Leu Leu Arg Asn Pro His Leu Val Ser Asp Tyr Arg Val Leu
85 90 95
Met Ala Glu Ile Gly Glu Asp Leu Asp Lys Ser Asp Val Ser Ser Leu
100 105 110
Ile Phe Leu Met Lys Asp Tyr Met Gly Arg Gly Lys Ile Ser Lys Glu
115 120 125
Lys Ser Phe Leu Asp Leu Val Val Glu Leu Glu Lys Leu Asn Leu Val
130 135 140
Ala Pro Asp Gln Leu Asp Leu Leu Glu Lys Cys Leu Lys Asn Ile His
145 150 155 160
Arg Ile Asp Leu Lys Thr Lys Ile Gln Lys Tyr Lys Gln Ser Val Gln
165 170 175
Gly Ala Gly Thr Ser Tyr Arg Asn Val Leu Gln Ala Ala Ile Gln Lys
180 185 190
Ser Leu Lys Asp Pro Ser Asn Asn Phe Arg Leu His Asn Gly Arg Ser
195 200 205
Lys Glu Gln Arg Leu Lys Glu Gln Leu Gly Ala Gln Gln Glu Pro Val
210 215 220
Lys Lys Ser Ile Gln Glu Ser Glu Ala Phe Leu Pro Gln Ser Ile Pro
225 230 235 240
Glu Glu Arg Tyr Lys Met Lys Ser Lys Pro Leu Gly Ile Cys Leu Ile
245 250 255
Ile Asp Cys Ile Gly Asn Glu Thr Glu Leu Leu Arg Asp Thr Phe Thr
260 265 270
Ser Leu Gly Tyr Glu Val Gln Lys Phe Leu His Leu Ser Met His Gly
275 280 285
Ile Ser Gln Ile Leu Gly Gln Phe Ala Cys Met Pro Glu His Arg Asp
290 295 300
Tyr Asp Ser Phe Val Cys Val Leu Val Ser Arg Gly Gly Ser Gln Ser
305 310 315 320
Val Tyr Gly Val Asp Gln Thr His Ser Gly Leu Pro Leu His His Ile
325 330 335
Arg Arg Met Phe Met Gly Asp Ser Cys Pro Tyr Leu Ala Gly Lys Pro
340 345 350
Lys Met Phe Phe Ile Gln Asn Tyr Val Val Ser Glu Gly Gln Leu Glu
355 360 365
Asp Ser Ser Leu Leu Glu Val Asp Gly Pro Ala Met Lys Asn Val Glu
370 375 380
Phe Lys Ala Gln Lys Arg Gly Leu Cys Thr Val His Arg Glu Ala Asp
385 390 395 400
Phe Phe Trp Ser Leu Cys Thr Ala Asp Met Ser Leu Leu Glu Gln Ser
405 410 415
His Ser Ser Pro Ser Leu Tyr Leu Gln Cys Leu Ser Gln Lys Leu Arg
420 425 430
Gln Glu Arg Lys Arg Pro Leu Leu Asp Leu His Ile Glu Leu Asn Gly
435 440 445
Tyr Met Tyr Asp Trp Asn Ser Arg Val Ser Ala Lys Glu Lys Tyr Tyr
450 455 460
Val Trp Leu Gln His Thr Leu Arg Lys Lys Leu Ile Leu Ser Tyr Thr
465 470 475 480
<210> 246
<211> 770
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 246
Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu
1 5 10 15
Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln
20 25 30
Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser
35 40 45
Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile
50 55 60
Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln
65 70 75 80
His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu
85 90 95
Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu
100 105 110
Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln
115 120 125
Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu
130 135 140
Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln
145 150 155 160
Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr
165 170 175
Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn
180 185 190
Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr
195 200 205
Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu
210 215 220
Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu
225 230 235 240
Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro
245 250 255
Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu
260 265 270
Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln
275 280 285
Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met
290 295 300
Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala
305 310 315 320
Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro
325 330 335
Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu
340 345 350
Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile
355 360 365
Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe
370 375 380
Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn
385 390 395 400
Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln
405 410 415
Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val
420 425 430
Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln
435 440 445
Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile
450 455 460
Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr
465 470 475 480
Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro
485 490 495
Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe
500 505 510
Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu
515 520 525
Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile
530 535 540
Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser
545 550 555 560
Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile
565 570 575
Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu
580 585 590
Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu
595 600 605
Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val
610 615 620
Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Phe
625 630 635 640
Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly
645 650 655
Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr
660 665 670
Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg
675 680 685
Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro
690 695 700
Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn
705 710 715 720
Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln
725 730 735
Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe
740 745 750
Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser
755 760 765
Pro Met
770
<210> 247
<211> 770
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 247
Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu
1 5 10 15
Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln
20 25 30
Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser
35 40 45
Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile
50 55 60
Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln
65 70 75 80
His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu
85 90 95
Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu
100 105 110
Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln
115 120 125
Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu
130 135 140
Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln
145 150 155 160
Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr
165 170 175
Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn
180 185 190
Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr
195 200 205
Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu
210 215 220
Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu
225 230 235 240
Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro
245 250 255
Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu
260 265 270
Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln
275 280 285
Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met
290 295 300
Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala
305 310 315 320
Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro
325 330 335
Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu
340 345 350
Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile
355 360 365
Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe
370 375 380
Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn
385 390 395 400
Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln
405 410 415
Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val
420 425 430
Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln
435 440 445
Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile
450 455 460
Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr
465 470 475 480
Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro
485 490 495
Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe
500 505 510
Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu
515 520 525
Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile
530 535 540
Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser
545 550 555 560
Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile
565 570 575
Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu
580 585 590
Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu
595 600 605
Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val
610 615 620
Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Tyr
625 630 635 640
Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly
645 650 655
Tyr Lys Ile Met Tyr Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr
660 665 670
Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg
675 680 685
Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro
690 695 700
Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn
705 710 715 720
Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln
725 730 735
Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe
740 745 750
Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser
755 760 765
Pro Met
770
<210> 248
<211> 770
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 248
Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu
1 5 10 15
Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln
20 25 30
Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser
35 40 45
Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile
50 55 60
Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln
65 70 75 80
His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu
85 90 95
Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu
100 105 110
Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln
115 120 125
Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu
130 135 140
Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln
145 150 155 160
Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr
165 170 175
Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn
180 185 190
Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr
195 200 205
Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu
210 215 220
Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu
225 230 235 240
Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro
245 250 255
Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu
260 265 270
Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln
275 280 285
Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met
290 295 300
Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala
305 310 315 320
Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro
325 330 335
Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu
340 345 350
Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile
355 360 365
Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe
370 375 380
Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn
385 390 395 400
Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln
405 410 415
Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val
420 425 430
Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln
435 440 445
Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile
450 455 460
Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr
465 470 475 480
Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro
485 490 495
Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe
500 505 510
Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu
515 520 525
Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile
530 535 540
Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser
545 550 555 560
Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile
565 570 575
Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu
580 585 590
Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu
595 600 605
Arg Phe Ser Glu Ser Arg Lys Glu Gly Gly Val Thr Phe Thr Trp Val
610 615 620
Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Phe
625 630 635 640
Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly
645 650 655
Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr
660 665 670
Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg
675 680 685
Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro
690 695 700
Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn
705 710 715 720
Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln
725 730 735
Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe
740 745 750
Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser
755 760 765
Pro Met
770
<210> 249
<211> 770
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 249
Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu
1 5 10 15
Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln
20 25 30
Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser
35 40 45
Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile
50 55 60
Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln
65 70 75 80
His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu
85 90 95
Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu
100 105 110
Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln
115 120 125
Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu
130 135 140
Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln
145 150 155 160
Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr
165 170 175
Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn
180 185 190
Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr
195 200 205
Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu
210 215 220
Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu
225 230 235 240
Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro
245 250 255
Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu
260 265 270
Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln
275 280 285
Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met
290 295 300
Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala
305 310 315 320
Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro
325 330 335
Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu
340 345 350
Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile
355 360 365
Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe
370 375 380
Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn
385 390 395 400
Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln
405 410 415
Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val
420 425 430
Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln
435 440 445
Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile
450 455 460
Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr
465 470 475 480
Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro
485 490 495
Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe
500 505 510
Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu
515 520 525
Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile
530 535 540
Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser
545 550 555 560
Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile
565 570 575
Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu
580 585 590
Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu
595 600 605
Arg Phe Ser Glu Ser Ser Lys Lys Gly Gly Val Thr Phe Thr Trp Val
610 615 620
Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Phe
625 630 635 640
Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly
645 650 655
Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr
660 665 670
Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg
675 680 685
Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro
690 695 700
Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn
705 710 715 720
Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln
725 730 735
Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe
740 745 750
Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser
755 760 765
Pro Met
770
<210> 250
<211> 770
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 250
Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu
1 5 10 15
Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln
20 25 30
Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser
35 40 45
Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile
50 55 60
Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln
65 70 75 80
His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu
85 90 95
Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu
100 105 110
Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln
115 120 125
Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu
130 135 140
Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln
145 150 155 160
Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr
165 170 175
Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn
180 185 190
Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr
195 200 205
Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu
210 215 220
Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu
225 230 235 240
Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro
245 250 255
Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu
260 265 270
Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln
275 280 285
Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met
290 295 300
Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala
305 310 315 320
Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro
325 330 335
Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu
340 345 350
Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile
355 360 365
Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe
370 375 380
Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn
385 390 395 400
Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln
405 410 415
Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val
420 425 430
Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln
435 440 445
Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile
450 455 460
Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr
465 470 475 480
Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro
485 490 495
Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe
500 505 510
Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu
515 520 525
Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile
530 535 540
Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser
545 550 555 560
Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile
565 570 575
Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu
580 585 590
Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu
595 600 605
Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val
610 615 620
Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Phe
625 630 635 640
Thr Lys Gln Gln Leu Asn Ile Met Ser Phe Ala Glu Ile Ile Met Gly
645 650 655
Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr
660 665 670
Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg
675 680 685
Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro
690 695 700
Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn
705 710 715 720
Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln
725 730 735
Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe
740 745 750
Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser
755 760 765
Pro Met
770
<210> 251
<211> 220
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 251
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val
1 5 10 15
Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr
20 25 30
Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser
35 40 45
Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu
50 55 60
Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser
65 70 75 80
Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr
85 90 95
Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys
100 105 110
Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Glu Asn Glu Lys Ser
115 120 125
Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro
130 135 140
Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly
145 150 155 160
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile
165 170 175
Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met
180 185 190
Asn Met Pro Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro
195 200 205
Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser
210 215 220
<210> 252
<211> 193
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 252
Met Ala Ala Ile Arg Lys Lys Leu Val Ile Val Gly Asp Gly Ala Cys
1 5 10 15
Val Lys Thr Cys Leu Leu Ile Val Phe Ser Lys Asp Gln Phe Pro Glu
20 25 30
Val Tyr Val Pro Thr Val Phe Glu Asn Tyr Val Ala Asp Ile Glu Val
35 40 45
Asp Gly Lys Gln Val Glu Leu Ala Leu Trp Asp Thr Ala Gly Gln Glu
50 55 60
Asp Tyr Asp Arg Leu Arg Pro Leu Ser Tyr Pro Asp Thr Asp Val Ile
65 70 75 80
Leu Met Cys Phe Ser Ile Asp Ser Pro Asp Ser Leu Glu Asn Ile Pro
85 90 95
Glu Lys Trp Thr Pro Glu Val Lys His Phe Cys Pro Asn Val Pro Ile
100 105 110
Ile Leu Val Gly Asn Lys Lys Asp Leu Arg Asn Asp Glu His Thr Arg
115 120 125
Arg Glu Leu Ala Lys Met Lys Gln Glu Pro Val Lys Pro Glu Glu Gly
130 135 140
Arg Asp Met Ala Asn Arg Ile Gly Ala Phe Gly Tyr Met Glu Cys Ser
145 150 155 160
Ala Lys Thr Lys Asp Gly Val Arg Glu Val Phe Glu Met Ala Thr Arg
165 170 175
Ala Ala Leu Gln Ala Arg Arg Gly Lys Lys Lys Ser Gly Cys Leu Val
180 185 190
Leu
<210> 253
<211> 193
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 253
Met Ala Ala Ile Arg Lys Lys Leu Val Ile Val Gly Asp Gly Ala Cys
1 5 10 15
Gly Asn Thr Cys Leu Leu Ile Val Phe Ser Lys Asp Gln Phe Pro Glu
20 25 30
Val Tyr Val Pro Thr Val Phe Glu Asn Tyr Val Ala Asp Ile Glu Val
35 40 45
Asp Gly Lys Gln Val Glu Leu Ala Leu Trp Asp Thr Ala Gly Gln Glu
50 55 60
Asp Tyr Asp Arg Leu Arg Pro Leu Ser Tyr Pro Asp Thr Asp Val Ile
65 70 75 80
Leu Met Cys Phe Ser Ile Asp Ser Pro Asp Ser Leu Glu Asn Ile Pro
85 90 95
Glu Lys Trp Thr Pro Glu Val Lys His Phe Cys Pro Asn Val Pro Ile
100 105 110
Ile Leu Val Gly Asn Lys Lys Asp Leu Arg Asn Asp Glu His Thr Arg
115 120 125
Arg Glu Leu Ala Lys Met Lys Gln Glu Pro Val Lys Pro Glu Glu Gly
130 135 140
Arg Asp Met Ala Asn Arg Ile Gly Ala Phe Gly Tyr Met Glu Cys Ser
145 150 155 160
Ala Lys Thr Lys Asp Gly Val Arg Glu Val Phe Glu Met Ala Thr Arg
165 170 175
Ala Ala Leu Gln Ala Arg Arg Gly Lys Lys Lys Ser Gly Cys Leu Val
180 185 190
Leu
<210> 254
<211> 1290
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 254
Met Ala Gly Ala Ala Ser Pro Cys Ala Asn Gly Cys Gly Pro Gly Ala
1 5 10 15
Pro Ser Asp Ala Glu Val Leu His Leu Cys Arg Ser Leu Glu Val Gly
20 25 30
Thr Val Met Thr Leu Phe Tyr Ser Lys Lys Ser Gln Arg Pro Glu Arg
35 40 45
Lys Thr Phe Gln Val Lys Leu Glu Thr Arg Gln Ile Thr Trp Ser Arg
50 55 60
Gly Ala Asp Lys Ile Glu Gly Ala Ile Asp Ile Arg Glu Ile Lys Glu
65 70 75 80
Ile Arg Pro Gly Lys Thr Ser Arg Asp Phe Asp Arg Tyr Gln Glu Asp
85 90 95
Pro Ala Phe Arg Pro Asp Gln Ser His Cys Phe Val Ile Leu Tyr Gly
100 105 110
Met Glu Phe Arg Leu Lys Thr Leu Ser Leu Gln Ala Thr Ser Glu Asp
115 120 125
Glu Val Asn Met Trp Ile Lys Gly Leu Thr Trp Leu Met Glu Asp Thr
130 135 140
Leu Gln Ala Pro Thr Pro Leu Gln Ile Glu Arg Trp Leu Arg Lys Gln
145 150 155 160
Phe Tyr Ser Val Asp Arg Asn Arg Glu Asp Arg Ile Ser Ala Lys Asp
165 170 175
Leu Lys Asn Met Leu Ser Gln Val Asn Tyr Arg Val Pro Asn Met Arg
180 185 190
Phe Leu Arg Glu Arg Leu Thr Asp Leu Glu Gln Arg Ser Gly Asp Ile
195 200 205
Thr Tyr Gly Gln Phe Ala Gln Leu Tyr Arg Ser Leu Met Tyr Ser Ala
210 215 220
Gln Lys Thr Met Asp Leu Pro Phe Leu Glu Ala Ser Thr Leu Arg Ala
225 230 235 240
Gly Glu Arg Pro Glu Leu Cys Arg Val Ser Leu Pro Glu Phe Gln Gln
245 250 255
Phe Leu Leu Asp Tyr Gln Gly Glu Leu Trp Ala Val Asp Arg Leu Gln
260 265 270
Val Gln Glu Phe Met Leu Ser Phe Leu Arg Asp Pro Leu Arg Glu Ile
275 280 285
Glu Glu Pro Tyr Phe Phe Leu Asp Glu Phe Val Thr Phe Leu Phe Ser
290 295 300
Lys Glu Asn Ser Val Trp Asn Ser Gln Leu Asp Ala Val Cys Pro Asp
305 310 315 320
Thr Met Asn Asn Pro Leu Ser His Tyr Trp Ile Ser Ser Ser His Asn
325 330 335
Thr Tyr Leu Thr Gly Asp Gln Phe Phe Ser Glu Ser Ser Leu Glu Ala
340 345 350
Tyr Ala Arg Cys Leu Arg Met Gly Cys Arg Cys Ile Glu Leu Asp Cys
355 360 365
Trp Asp Gly Pro Asp Gly Met Pro Val Ile Tyr His Gly His Thr Leu
370 375 380
Thr Thr Lys Ile Lys Phe Ser Asp Val Leu His Thr Ile Lys Glu His
385 390 395 400
Ala Phe Val Ala Ser Glu Tyr Pro Val Ile Leu Ser Ile Glu Asp His
405 410 415
Cys Ser Ile Ala Gln Gln Arg Asn Met Ala Gln Tyr Phe Lys Lys Val
420 425 430
Leu Gly Asp Thr Leu Leu Thr Lys Pro Val Glu Ile Ser Ala Asp Gly
435 440 445
Leu Pro Ser Pro Asn Gln Leu Lys Arg Lys Ile Leu Ile Lys His Lys
450 455 460
Lys Leu Ala Glu Gly Ser Ala Tyr Glu Glu Val Pro Thr Ser Met Met
465 470 475 480
Tyr Ser Glu Asn Asp Ile Ser Asn Ser Ile Lys Asn Gly Ile Leu Tyr
485 490 495
Leu Glu Asp Pro Val Asn His Glu Trp Tyr Pro His Tyr Phe Val Leu
500 505 510
Thr Ser Ser Lys Ile Tyr Tyr Ser Glu Glu Thr Ser Ser Asp Gln Gly
515 520 525
Asn Glu Asp Glu Glu Glu Pro Lys Glu Val Ser Ser Ser Thr Glu Leu
530 535 540
His Ser Asn Glu Lys Trp Phe His Gly Lys Leu Gly Ala Gly Arg Asp
545 550 555 560
Gly Arg His Ile Ala Glu Arg Leu Leu Thr Glu Tyr Cys Ile Glu Thr
565 570 575
Gly Ala Pro Asp Gly Ser Phe Leu Val Arg Glu Ser Glu Thr Phe Val
580 585 590
Gly Asp Tyr Thr Leu Ser Phe Trp Arg Asn Gly Lys Val Gln His Cys
595 600 605
Arg Ile His Ser Arg Gln Asp Ala Gly Thr Pro Lys Phe Phe Leu Thr
610 615 620
Asp Asn Leu Val Phe Asp Ser Leu Tyr Asp Leu Ile Thr His Tyr Gln
625 630 635 640
Gln Val Pro Leu Arg Cys Asn Glu Phe Glu Met Arg Leu Ser Glu Pro
645 650 655
Val Pro Gln Thr Asn Ala His Glu Ser Lys Glu Trp Tyr His Ala Ser
660 665 670
Leu Thr Arg Ala Gln Ala Glu His Met Leu Met Arg Val Pro Arg Asp
675 680 685
Gly Ala Phe Leu Val Arg Lys Arg Asn Glu Pro Asn Ser Tyr Ala Ile
690 695 700
Ser Phe Arg Ala Glu Gly Lys Ile Lys His Cys Arg Val Gln Gln Glu
705 710 715 720
Gly Gln Thr Val Met Leu Gly Asn Ser Glu Phe Asp Ser Leu Val Asp
725 730 735
Leu Ile Ser Tyr Tyr Glu Lys His Pro Leu Tyr Arg Lys Met Lys Leu
740 745 750
Arg Tyr Pro Ile Asn Glu Glu Ala Leu Glu Lys Ile Gly Thr Ala Glu
755 760 765
Pro Asp Tyr Gly Ala Leu Tyr Glu Gly Arg Asn Pro Gly Phe Tyr Val
770 775 780
Glu Ala Asn Pro Met Pro Thr Phe Lys Cys Ala Val Lys Ala Leu Phe
785 790 795 800
Asp Tyr Lys Ala Gln Arg Glu Asp Glu Leu Thr Phe Ile Lys Ser Ala
805 810 815
Ile Ile Gln Asn Val Glu Lys Gln Glu Gly Gly Trp Trp Arg Gly Asp
820 825 830
Tyr Gly Gly Lys Lys Gln Leu Trp Phe Pro Ser Asn Tyr Val Glu Glu
835 840 845
Met Val Asn Pro Val Ala Leu Glu Pro Glu Arg Glu His Leu Asp Glu
850 855 860
Asn Ser Pro Leu Gly Asp Leu Leu Arg Gly Val Leu Asp Val Pro Ala
865 870 875 880
Cys Gln Ile Ala Ile Arg Pro Glu Gly Lys Asn Asn Arg Leu Phe Val
885 890 895
Phe Ser Ile Ser Met Ala Ser Val Ala His Trp Ser Leu Asp Val Ala
900 905 910
Ala Asp Ser Gln Glu Glu Leu Gln Asp Trp Val Lys Lys Ile Arg Glu
915 920 925
Val Ala Gln Thr Ala Asp Ala Arg Leu Thr Glu Gly Lys Ile Met Glu
930 935 940
Arg Arg Lys Lys Ile Ala Leu Glu Leu Ser Glu Leu Val Val Tyr Cys
945 950 955 960
Arg Pro Val Pro Phe Asp Glu Glu Lys Ile Gly Thr Glu Arg Ala Cys
965 970 975
Tyr Arg Asp Met Ser Ser Phe Pro Glu Thr Lys Ala Glu Lys Tyr Val
980 985 990
Asn Lys Ala Lys Gly Lys Lys Phe Leu Gln Tyr Asn Arg Leu Gln Leu
995 1000 1005
Ser Arg Ile Tyr Pro Lys Gly Gln Arg Leu Asp Ser Ser Asn Tyr
1010 1015 1020
Asp Pro Leu Pro Met Trp Ile Cys Gly Ser Gln Leu Val Ala Leu
1025 1030 1035
Asn Phe Gln Thr Pro Asp Lys Pro Met Gln Met Asn Gln Ala Leu
1040 1045 1050
Phe Met Thr Gly Arg His Cys Gly Tyr Val Leu Gln Pro Ser Thr
1055 1060 1065
Met Arg Asp Glu Ala Phe Asp Pro Phe Asp Lys Ser Ser Leu Arg
1070 1075 1080
Gly Leu Glu Pro Cys Ala Ile Ser Ile Glu Val Leu Gly Ala Arg
1085 1090 1095
His Leu Pro Lys Asn Gly Arg Gly Ile Val Cys Pro Phe Val Glu
1100 1105 1110
Ile Glu Val Ala Gly Ala Glu Tyr Asp Ser Thr Lys Gln Lys Thr
1115 1120 1125
Glu Phe Val Val Asp Asn Gly Leu Asn Pro Val Trp Pro Ala Lys
1130 1135 1140
Pro Phe His Phe Gln Ile Ser Asn Pro Glu Phe Ala Phe Leu Arg
1145 1150 1155
Phe Val Val Tyr Glu Glu Asp Met Phe Ser Asp Gln Asn Phe Leu
1160 1165 1170
Ala Gln Ala Thr Phe Pro Val Lys Gly Leu Lys Thr Gly Tyr Arg
1175 1180 1185
Ala Val Pro Leu Lys Asn Asn Tyr Ser Glu Asp Leu Glu Leu Ala
1190 1195 1200
Ser Leu Leu Ile Lys Ile Asp Ile Phe Pro Ala Lys Glu Asn Gly
1205 1210 1215
Asp Leu Ser Pro Phe Ser Gly Thr Ser Leu Arg Glu Arg Gly Ser
1220 1225 1230
Asp Ala Ser Gly Gln Leu Phe His Gly Arg Ala Arg Glu Gly Ser
1235 1240 1245
Phe Glu Ser Arg Tyr Gln Gln Pro Phe Glu Asp Phe Arg Ile Ser
1250 1255 1260
Gln Glu His Leu Ala Asp His Phe Asp Ser Arg Glu Arg Arg Ala
1265 1270 1275
Pro Arg Arg Thr Arg Val Asn Gly Asp Asn Arg Leu
1280 1285 1290
<210> 255
<211> 787
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 255
Met Ala Val Trp Ile Gln Ala Gln Gln Leu Gln Gly Glu Ala Leu His
1 5 10 15
Gln Met Gln Ala Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His
20 25 30
Tyr Leu Ser Gln Trp Ile Glu Ser Gln Ala Trp Asp Ser Val Asp Leu
35 40 45
Asp Asn Pro Gln Glu Asn Ile Lys Ala Thr Gln Leu Leu Glu Gly Leu
50 55 60
Val Gln Glu Leu Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly
65 70 75 80
Phe Leu Leu Lys Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Asn
85 90 95
Thr Tyr Asp Arg Cys Pro Met Glu Leu Val Arg Cys Ile Arg His Ile
100 105 110
Leu Tyr Asn Glu Gln Arg Leu Val Arg Glu Ala Asn Asn Gly Ser Ser
115 120 125
Pro Ala Gly Ser Leu Ala Asp Ala Met Ser Gln Lys His Leu Gln Ile
130 135 140
Asn Gln Thr Phe Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn
145 150 155 160
Glu Leu Lys Lys Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr
165 170 175
Gln Glu Ser Leu Arg Ile Gln Ala Gln Phe Gly Pro Leu Ala Gln Leu
180 185 190
Ser Pro Gln Glu Arg Leu Ser Arg Glu Thr Ala Leu Gln Gln Lys Gln
195 200 205
Val Ser Leu Glu Ala Trp Leu Gln Arg Glu Ala Gln Thr Leu Gln Gln
210 215 220
Tyr Arg Val Glu Leu Ala Glu Lys His Gln Lys Thr Leu Gln Leu Leu
225 230 235 240
Arg Lys Gln Gln Thr Ile Ile Leu Asp Asp Glu Leu Ile Gln Trp Lys
245 250 255
Arg Arg Gln Gln Leu Ala Gly Asn Gly Gly Pro Pro Glu Gly Ser Leu
260 265 270
Asp Val Leu Gln Ser Trp Cys Glu Lys Leu Ala Glu Ile Ile Trp Gln
275 280 285
Asn Arg Gln Gln Ile Arg Arg Ala Glu His Leu Cys Gln Gln Leu Pro
290 295 300
Ile Pro Gly Pro Val Glu Glu Met Leu Ala Glu Val Asn Ala Thr Ile
305 310 315 320
Thr Asp Ile Ile Ser Ala Leu Val Thr Ser Thr Phe Ile Ile Glu Lys
325 330 335
Gln Pro Pro Gln Val Leu Lys Thr Gln Thr Lys Phe Ala Ala Thr Val
340 345 350
Arg Leu Leu Val Gly Gly Lys Leu Asn Val His Met Asn Pro Pro Gln
355 360 365
Val Lys Ala Thr Ile Ile Ser Glu Gln Gln Ala Lys Ser Leu Leu Lys
370 375 380
Asn Glu Asn Thr Arg Asn Asp Tyr Ser Gly Glu Ile Leu Asn Asn Cys
385 390 395 400
Cys Val Met Glu Tyr His Gln Ala Thr Gly Thr Leu Ser Ala His Phe
405 410 415
Arg Asn Met Ser Leu Lys Arg Ile Lys Arg Ser Asp Arg Arg Gly Ala
420 425 430
Glu Ser Val Thr Glu Glu Lys Phe Thr Ile Leu Phe Glu Ser Gln Phe
435 440 445
Ser Val Gly Gly Asn Glu Leu Val Phe Gln Val Lys Thr Leu Ser Leu
450 455 460
Pro Val Val Val Ile Val His Gly Ser Gln Asp Asn Asn Ala Thr Ala
465 470 475 480
Thr Val Leu Trp Asp Asn Ala Phe Ala Glu Pro Gly Arg Val Pro Phe
485 490 495
Ala Val Pro Asp Lys Val Leu Trp Pro Gln Leu Cys Glu Ala Leu Asn
500 505 510
Met Lys Phe Lys Ala Glu Val Gln Ser Asn Arg Gly Leu Thr Lys Glu
515 520 525
Asn Leu Val Phe Leu Ala Gln Lys Leu Phe Asn Asn Ser Ser Ser His
530 535 540
Leu Glu Asp Tyr Ser Gly Leu Ser Val Ser Trp Ser Gln Phe Asn Arg
545 550 555 560
Glu Asn Leu Pro Gly Arg Asn Tyr Thr Phe Trp Gln Trp Phe Asp Gly
565 570 575
Val Met Glu Val Leu Lys Lys His Leu Lys Pro His Trp Asn Asp Gly
580 585 590
Ala Ile Leu Gly Phe Val Asn Lys Gln Gln Ala His Asp Leu Leu Ile
595 600 605
Asn Lys Pro Asp Gly Thr Phe Leu Leu Arg Phe Ser Asp Ser Glu Ile
610 615 620
Gly Gly Ile Thr Ile Ala Trp Lys Phe Asp Ser Gln Glu Arg Met Phe
625 630 635 640
Trp His Leu Met Pro Phe Thr Thr Arg Asp Phe Ser Ile Arg Ser Leu
645 650 655
Ala Asp Arg Leu Gly Asp Leu Asn Tyr Leu Ile Tyr Val Phe Pro Asp
660 665 670
Arg Pro Lys Asp Glu Val Tyr Ser Lys Tyr Tyr Thr Pro Val Pro Cys
675 680 685
Glu Ser Ala Thr Ala Lys Ala Val Asp Gly Tyr Val Lys Pro Gln Ile
690 695 700
Lys Gln Val Val Pro Glu Phe Val Asn Ala Ser Ala Asp Ala Gly Gly
705 710 715 720
Gly Ser Ala Thr Tyr Met Asp Gln Ala Pro Ser Pro Ala Val Cys Pro
725 730 735
Gln Ala His Tyr Asn Met Tyr Pro Gln Asn Pro Asp Ser Val Leu Asp
740 745 750
Thr Asp Gly Asp Phe Asp Leu Glu Asp Thr Met Asp Val Ala Arg Arg
755 760 765
Val Glu Glu Leu Leu Gly Arg Pro Met Asp Ser Gln Trp Ile Pro His
770 775 780
Ala Gln Ser
785
<210> 256
<211> 295
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 256
Met Glu His Gln Leu Leu Cys Cys Glu Val Glu Thr Ile Arg Arg Ala
1 5 10 15
Tyr Pro Asp Ala Asn Leu Leu Asn Asp Arg Val Leu Arg Ala Met Leu
20 25 30
Lys Ala Glu Glu Thr Cys Ala Pro Leu Val Ser Tyr Phe Lys Cys Val
35 40 45
Gln Lys Glu Val Leu Pro Ser Met Arg Lys Ile Val Ala Thr Trp Met
50 55 60
Leu Glu Val Cys Glu Glu Gln Lys Cys Glu Glu Glu Val Phe Pro Leu
65 70 75 80
Ala Met Asn Tyr Leu Asp Arg Phe Leu Ser Leu Glu Pro Val Lys Lys
85 90 95
Ser Arg Leu Gln Leu Leu Gly Ala Thr Cys Met Phe Val Ala Ser Lys
100 105 110
Met Lys Glu Thr Ile Pro Leu Thr Ala Glu Lys Leu Cys Ile Tyr Thr
115 120 125
Asp Asn Ser Ile Arg Pro Glu Glu Leu Leu Gln Met Glu Leu Leu Leu
130 135 140
Val Asn Lys Leu Lys Trp Asn Leu Ala Ala Met Thr Pro His Asp Phe
145 150 155 160
Ile Glu His Phe Leu Ser Lys Met Pro Glu Ala Glu Glu Asn Lys Gln
165 170 175
Ile Ile Arg Lys His Ala Gln Thr Phe Val Ala Leu Cys Ala Thr Asp
180 185 190
Val Lys Phe Ile Ser Asn Pro Pro Ser Met Val Ala Ala Gly Ser Val
195 200 205
Val Ala Ala Val Gln Gly Leu Asn Leu Arg Ser Pro Asn Asn Phe Leu
210 215 220
Ser Tyr Tyr Arg Leu Thr Arg Phe Leu Ser Arg Val Ile Lys Cys Asp
225 230 235 240
Pro Asp Cys Leu Arg Ala Cys Gln Glu Gln Ile Glu Ala Leu Leu Glu
245 250 255
Ser Ser Leu Arg Gln Ala Gln Gln Asn Met Asp Pro Lys Ala Ala Glu
260 265 270
Glu Glu Glu Glu Glu Glu Glu Glu Val Asp Leu Ala Cys Thr Pro Thr
275 280 285
Asp Val Arg Asp Val Asp Ile
290 295
<210> 257
<211> 1068
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 257
Met Pro Pro Arg Pro Ser Ser Gly Glu Leu Trp Gly Ile His Leu Met
1 5 10 15
Pro Pro Arg Ile Leu Val Glu Cys Leu Leu Pro Asn Gly Met Ile Val
20 25 30
Thr Leu Glu Cys Leu Arg Glu Ala Thr Leu Ile Thr Ile Lys His Glu
35 40 45
Leu Phe Lys Glu Ala Arg Lys Tyr Pro Leu His Gln Leu Leu Gln Asp
50 55 60
Glu Ser Ser Tyr Ile Phe Val Ser Val Thr Gln Glu Ala Glu Arg Glu
65 70 75 80
Glu Phe Phe Asp Glu Thr Arg Arg Leu Cys Asp Leu Arg Leu Phe Gln
85 90 95
Pro Phe Leu Lys Val Ile Glu Pro Val Gly Asn Arg Glu Glu Lys Ile
100 105 110
Leu Asn Arg Glu Ile Gly Phe Ala Ile Gly Met Pro Val Cys Glu Phe
115 120 125
Asp Met Val Lys Asp Pro Glu Val Gln Asp Phe Arg Arg Asn Ile Leu
130 135 140
Asn Val Cys Lys Glu Ala Val Asp Leu Arg Asp Leu Asn Ser Pro His
145 150 155 160
Ser Arg Ala Met Tyr Val Tyr Pro Pro Asn Val Glu Ser Ser Pro Glu
165 170 175
Leu Pro Lys His Ile Tyr Asn Lys Leu Asp Lys Gly Gln Ile Ile Val
180 185 190
Val Ile Trp Val Ile Val Ser Pro Asn Asn Asp Lys Gln Lys Tyr Thr
195 200 205
Leu Lys Ile Asn His Asp Cys Val Pro Glu Gln Val Ile Ala Glu Ala
210 215 220
Ile Arg Lys Lys Thr Arg Ser Met Leu Leu Ser Ser Glu Gln Leu Lys
225 230 235 240
Leu Cys Val Leu Glu Tyr Gln Gly Lys Tyr Ile Leu Lys Val Cys Gly
245 250 255
Cys Asp Glu Tyr Phe Leu Glu Lys Tyr Pro Leu Ser Gln Tyr Lys Tyr
260 265 270
Ile Arg Ser Cys Ile Met Leu Gly Arg Met Pro Asn Leu Met Leu Met
275 280 285
Ala Lys Glu Ser Leu Tyr Ser Gln Leu Pro Met Asp Cys Phe Thr Met
290 295 300
Pro Ser Tyr Ser Arg Arg Ile Ser Thr Ala Thr Pro Tyr Met Asn Gly
305 310 315 320
Glu Thr Ser Thr Lys Ser Leu Trp Val Ile Asn Ser Ala Leu Arg Ile
325 330 335
Lys Ile Leu Cys Ala Thr Tyr Val Asn Val Asn Ile Arg Asp Ile Asp
340 345 350
Lys Ile Tyr Val Arg Thr Gly Ile Tyr His Gly Gly Glu Pro Leu Cys
355 360 365
Asp Asn Val Asn Thr Gln Arg Val Pro Cys Ser Asn Pro Arg Trp Asn
370 375 380
Glu Trp Leu Asn Tyr Asp Ile Tyr Ile Pro Asp Leu Pro Arg Ala Ala
385 390 395 400
Arg Leu Cys Leu Ser Ile Cys Ser Val Lys Gly Arg Lys Gly Ala Lys
405 410 415
Glu Glu His Cys Pro Leu Ala Trp Gly Asn Ile Asn Leu Phe Asp Tyr
420 425 430
Thr Asp Thr Leu Val Ser Gly Lys Met Ala Leu Asn Leu Trp Pro Val
435 440 445
Pro His Gly Leu Glu Asp Leu Leu Asn Pro Ile Gly Val Thr Gly Ser
450 455 460
Asn Pro Asn Lys Glu Thr Pro Cys Leu Glu Leu Glu Phe Asp Trp Phe
465 470 475 480
Ser Ser Val Val Lys Phe Pro Asp Met Ser Val Ile Glu Glu His Ala
485 490 495
Asn Trp Ser Val Ser Arg Glu Ala Gly Phe Ser Tyr Ser His Ala Gly
500 505 510
Leu Ser Asn Arg Leu Ala Arg Asp Asn Glu Leu Arg Glu Asn Asp Lys
515 520 525
Glu Gln Leu Lys Ala Ile Ser Thr Arg Asp Pro Leu Ser Glu Ile Thr
530 535 540
Glu Gln Glu Lys Asp Phe Leu Trp Ser His Arg His Tyr Cys Val Thr
545 550 555 560
Ile Pro Glu Ile Leu Pro Lys Leu Leu Leu Ser Val Lys Trp Asn Ser
565 570 575
Arg Asp Glu Val Ala Gln Met Tyr Cys Leu Val Lys Asp Trp Pro Pro
580 585 590
Ile Lys Pro Glu Gln Ala Met Glu Leu Leu Asp Cys Asn Tyr Pro Asp
595 600 605
Pro Met Val Arg Gly Phe Ala Val Arg Cys Leu Glu Lys Tyr Leu Thr
610 615 620
Asp Asp Lys Leu Ser Gln Tyr Leu Ile Gln Leu Val Gln Val Leu Lys
625 630 635 640
Tyr Glu Gln Tyr Leu Asp Asn Leu Leu Val Arg Phe Leu Leu Lys Lys
645 650 655
Ala Leu Thr Asn Gln Arg Ile Gly His Phe Phe Phe Trp His Leu Lys
660 665 670
Ser Glu Met His Asn Lys Thr Val Ser Gln Arg Phe Gly Leu Leu Leu
675 680 685
Glu Ser Tyr Cys Arg Ala Cys Gly Met Tyr Leu Lys His Leu Asn Arg
690 695 700
Gln Val Glu Ala Met Glu Lys Leu Ile Asn Leu Thr Asp Ile Leu Lys
705 710 715 720
Gln Glu Lys Lys Asp Glu Thr Gln Lys Val Gln Met Lys Phe Leu Val
725 730 735
Glu Gln Met Arg Arg Pro Asp Phe Met Asp Ala Leu Gln Gly Phe Leu
740 745 750
Ser Pro Leu Asn Pro Ala His Gln Leu Gly Asn Leu Arg Leu Glu Glu
755 760 765
Cys Arg Ile Met Ser Ser Ala Lys Arg Pro Leu Trp Leu Asn Trp Glu
770 775 780
Asn Pro Asp Ile Met Ser Glu Leu Leu Phe Gln Asn Asn Glu Ile Ile
785 790 795 800
Phe Lys Asn Gly Asp Asp Leu Arg Gln Asp Met Leu Thr Leu Gln Ile
805 810 815
Ile Arg Ile Met Glu Asn Ile Trp Gln Asn Gln Gly Leu Asp Leu Arg
820 825 830
Met Leu Pro Tyr Gly Cys Leu Ser Ile Gly Asp Cys Val Gly Leu Ile
835 840 845
Glu Val Val Arg Asn Ser His Thr Ile Met Gln Ile Gln Cys Lys Gly
850 855 860
Gly Leu Lys Gly Ala Leu Gln Phe Asn Ser His Thr Leu His Gln Trp
865 870 875 880
Leu Lys Asp Lys Asn Lys Gly Glu Ile Tyr Asp Ala Ala Ile Asp Leu
885 890 895
Phe Thr Arg Ser Cys Ala Gly Tyr Cys Val Ala Thr Phe Ile Leu Gly
900 905 910
Ile Gly Asp Arg His Asn Ser Asn Ile Met Val Lys Asp Asp Gly Gln
915 920 925
Leu Phe His Ile Asp Phe Gly His Phe Leu Asp His Lys Lys Lys Lys
930 935 940
Phe Gly Tyr Lys Arg Glu Arg Val Pro Phe Val Leu Thr Gln Asp Phe
945 950 955 960
Leu Ile Val Ile Ser Lys Gly Ala Gln Glu Cys Thr Lys Thr Arg Glu
965 970 975
Phe Glu Arg Phe Gln Glu Met Cys Tyr Lys Ala Tyr Leu Ala Ile Arg
980 985 990
Gln His Ala Asn Leu Phe Ile Asn Pro Phe Ser Met Met Leu Gly Ser
995 1000 1005
Gly Met Pro Glu Leu Gln Ser Phe Asp Asp Ile Ala Tyr Ile Arg
1010 1015 1020
Lys Thr Leu Ala Leu Asp Lys Thr Glu Gln Glu Ala Leu Glu Tyr
1025 1030 1035
Phe Met Lys Gln Met Asn Asp Ala His His Gly Gly Trp Thr Thr
1040 1045 1050
Lys Met Asp Trp Ile Phe His Thr Ile Lys Gln His Ala Leu Asn
1055 1060 1065
<210> 258
<211> 15
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 258
Met Thr Ala Ile Ile Lys Glu Ile Val Ser Arg Asn Lys Arg Arg
1 5 10 15
<210> 259
<211> 20
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 259
Met Thr Val Glu Glu Pro Ser Asn Pro Glu Ala Ser Ser Ser Thr Ser
1 5 10 15
Val Thr Pro Asp
20
<210> 260
<211> 526
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 260
Met Gly Ser Ser Lys Ser Lys Pro Lys Asp Pro Ser Gln Arg Arg Cys
1 5 10 15
Ser Leu Glu Pro Pro Asp Ser Thr His His Gly Gly Phe Pro Ala Ser
20 25 30
Gln Thr Pro Asn Lys Thr Ala Ala Pro Asp Thr His Arg Thr Pro Ser
35 40 45
Arg Ser Phe Gly Thr Val Ala Thr Glu Pro Lys Leu Phe Gly Gly Phe
50 55 60
Asn Thr Ser Asp Thr Val Thr Ser Pro Gln Arg Ala Gly Ala Leu Ala
65 70 75 80
Gly Gly Val Thr Thr Phe Val Ala Leu Tyr Asp Tyr Glu Ser Arg Thr
85 90 95
Glu Thr Asp Leu Ser Phe Lys Lys Gly Glu Arg Leu Gln Ile Val Asn
100 105 110
Asn Thr Glu Gly Asp Trp Trp Leu Ala His Ser Leu Thr Thr Gly Gln
115 120 125
Thr Gly Tyr Ile Pro Ser Asn Tyr Val Ala Pro Ser Asp Ser Ile Gln
130 135 140
Ala Glu Glu Trp Tyr Phe Gly Lys Ile Thr Arg Arg Glu Ser Glu Arg
145 150 155 160
Leu Leu Leu Asn Pro Glu Asn Pro Arg Gly Thr Phe Leu Val Arg Glu
165 170 175
Ser Glu Thr Thr Lys Gly Ala Tyr Cys Leu Ser Val Ser Asp Phe Asp
180 185 190
Asn Ala Lys Gly Leu Asn Val Lys His Tyr Lys Ile Arg Lys Leu Asp
195 200 205
Ser Gly Gly Phe Tyr Ile Thr Ser Arg Thr Gln Phe Ser Ser Leu Gln
210 215 220
Gln Leu Val Ala Tyr Tyr Ser Lys His Ala Asp Gly Leu Cys His Arg
225 230 235 240
Leu Thr Asn Val Cys Pro Thr Ser Lys Pro Gln Thr Gln Gly Leu Ala
245 250 255
Lys Asp Ala Trp Glu Ile Pro Arg Glu Ser Leu Arg Leu Glu Val Lys
260 265 270
Leu Gly Gln Gly Cys Phe Gly Glu Val Trp Met Gly Thr Trp Asn Gly
275 280 285
Thr Thr Arg Val Ala Ile Lys Thr Leu Lys Pro Gly Thr Met Ser Pro
290 295 300
Glu Ala Phe Leu Gln Glu Ala Gln Val Met Lys Lys Leu Arg His Glu
305 310 315 320
Lys Leu Val Gln Leu Tyr Ala Val Val Ser Glu Glu Pro Ile Tyr Ile
325 330 335
Val Thr Glu Tyr Met Ser Lys Gly Ser Leu Leu Asp Phe Leu Lys Gly
340 345 350
Glu Met Gly Lys Tyr Leu Arg Leu Pro Gln Leu Val Asp Met Ala Ala
355 360 365
Gln Ile Ala Ser Gly Met Ala Tyr Val Glu Arg Met Asn Tyr Val His
370 375 380
Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Gly Glu Asn Leu Val Cys
385 390 395 400
Lys Val Ala Asp Phe Gly Leu Ala Arg Leu Ile Glu Asp Asn Glu Tyr
405 410 415
Thr Ala Arg Gln Gly Ala Lys Phe Pro Ile Lys Trp Thr Ala Pro Glu
420 425 430
Ala Ala Leu Tyr Gly Arg Phe Thr Ile Lys Ser Asp Val Trp Ser Phe
435 440 445
Gly Ile Leu Leu Thr Glu Leu Thr Thr Lys Gly Arg Val Pro Tyr Pro
450 455 460
Gly Met Gly Asn Gly Glu Val Leu Asp Arg Val Glu Arg Gly Tyr Arg
465 470 475 480
Met Pro Cys Pro Pro Glu Cys Pro Glu Ser Leu His Asp Leu Met Cys
485 490 495
Gln Cys Trp Arg Arg Asp Pro Glu Glu Arg Pro Thr Phe Glu Tyr Leu
500 505 510
Gln Ala Gln Leu Leu Pro Ala Cys Val Leu Glu Val Ala Glu
515 520 525
<210> 261
<211> 277
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 261
Met Glu Asn Thr Glu Asn Ser Val Asp Ser Lys Ser Ile Lys Asn Leu
1 5 10 15
Glu Pro Lys Ile Ile His Gly Ser Glu Ser Met Asp Ser Gly Ile Ser
20 25 30
Leu Asp Asn Ser Tyr Lys Met Asp Tyr Pro Glu Met Gly Leu Cys Ile
35 40 45
Ile Ile Asn Asn Lys Asn Phe His Lys Ser Thr Gly Met Thr Ser Arg
50 55 60
Ser Gly Thr Asp Val Asp Ala Ala Asn Leu Arg Glu Thr Phe Arg Asn
65 70 75 80
Leu Lys Tyr Glu Val Arg Asn Lys Asn Asp Leu Thr Arg Glu Glu Ile
85 90 95
Val Glu Leu Met Arg Asp Val Ser Lys Glu Asp His Ser Lys Arg Ser
100 105 110
Ser Phe Val Cys Val Leu Leu Ser His Gly Glu Glu Gly Ile Ile Phe
115 120 125
Gly Thr Asn Gly Pro Val Asp Leu Lys Lys Ile Thr Asn Phe Phe Arg
130 135 140
Gly Asp Arg Cys Arg Ser Leu Thr Gly Lys Pro Lys Leu Phe Ile Ile
145 150 155 160
Gln Ala Ala Arg Gly Thr Glu Leu Asp Cys Gly Ile Glu Thr Asp Ser
165 170 175
Gly Val Asp Asp Asp Met Ala Cys His Lys Ile Pro Val Glu Ala Asp
180 185 190
Phe Leu Tyr Ala Tyr Ser Thr Ala Pro Gly Tyr Tyr Ser Trp Arg Asn
195 200 205
Ser Lys Asp Gly Ser Trp Phe Ile Gln Ser Leu Cys Ala Met Leu Lys
210 215 220
Gln Tyr Ala Asp Lys Leu Glu Phe Met His Ile Leu Thr Arg Val Asn
225 230 235 240
Arg Lys Val Ala Thr Glu Phe Glu Ser Phe Ser Phe Asp Ala Thr Phe
245 250 255
His Ala Lys Lys Gln Ile Pro Cys Ile Val Ser Met Leu Thr Lys Glu
260 265 270
Leu Tyr Phe Tyr His
275
<210> 262
<211> 303
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 262
Met Ala Asp Asp Gln Gly Cys Ile Glu Glu Gln Gly Val Glu Asp Ser
1 5 10 15
Ala Asn Glu Asp Ser Val Asp Ala Lys Pro Asp Arg Ser Ser Phe Val
20 25 30
Pro Ser Leu Phe Ser Lys Lys Lys Lys Asn Val Thr Met Arg Ser Ile
35 40 45
Lys Thr Thr Arg Asp Arg Val Pro Thr Tyr Gln Tyr Asn Met Asn Phe
50 55 60
Glu Lys Leu Gly Lys Cys Ile Ile Ile Asn Asn Lys Asn Phe Asp Lys
65 70 75 80
Val Thr Gly Met Gly Val Arg Asn Gly Thr Asp Lys Asp Ala Glu Ala
85 90 95
Leu Phe Lys Cys Phe Arg Ser Leu Gly Phe Asp Val Ile Val Tyr Asn
100 105 110
Asp Cys Ser Cys Ala Lys Met Gln Asp Leu Leu Lys Lys Ala Ser Glu
115 120 125
Glu Asp His Thr Asn Ala Ala Cys Phe Ala Cys Ile Leu Leu Ser His
130 135 140
Gly Glu Glu Asn Val Ile Tyr Gly Lys Asp Gly Val Thr Pro Ile Lys
145 150 155 160
Asp Leu Thr Ala His Phe Arg Gly Asp Arg Cys Lys Thr Leu Leu Glu
165 170 175
Lys Pro Lys Leu Phe Phe Ile Gln Ala Ala Arg Gly Thr Glu Leu Asp
180 185 190
Asp Gly Ile Gln Ala Asp Ser Gly Pro Ile Asn Asp Thr Asp Ala Asn
195 200 205
Pro Arg Tyr Lys Ile Pro Val Glu Ala Asp Phe Leu Phe Ala Tyr Ser
210 215 220
Thr Val Pro Gly Tyr Tyr Ser Trp Arg Ser Pro Gly Arg Gly Ser Trp
225 230 235 240
Phe Val Gln Ala Leu Cys Ser Ile Leu Glu Glu His Gly Lys Asp Leu
245 250 255
Glu Ile Met Gln Ile Leu Thr Arg Val Asn Asp Arg Val Ala Arg His
260 265 270
Phe Glu Ser Gln Ser Asp Asp Pro His Phe His Glu Lys Lys Gln Ile
275 280 285
Pro Cys Val Val Ser Met Leu Thr Lys Glu Leu Tyr Phe Ser Gln
290 295 300
<210> 263
<211> 479
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 263
Met Asp Phe Ser Arg Asn Leu Tyr Asp Ile Gly Glu Gln Leu Asp Ser
1 5 10 15
Glu Asp Leu Ala Ser Leu Lys Phe Leu Ser Leu Asp Tyr Ile Pro Gln
20 25 30
Arg Lys Gln Glu Pro Ile Lys Asp Ala Leu Met Leu Phe Gln Arg Leu
35 40 45
Gln Glu Lys Arg Met Leu Glu Glu Ser Asn Leu Ser Phe Leu Lys Glu
50 55 60
Leu Leu Phe Arg Ile Asn Arg Leu Ala Leu Leu Ile Thr Tyr Leu Asn
65 70 75 80
Thr Arg Lys Glu Glu Met Glu Arg Glu Leu Gln Thr Pro Gly Arg Ala
85 90 95
Gln Ile Ser Ala Tyr Arg Val Met Leu Tyr Gln Ile Ser Glu Glu Val
100 105 110
Ser Arg Ser Glu Leu Arg Ser Phe Lys Phe Leu Leu Gln Glu Glu Ile
115 120 125
Ser Lys Cys Lys Leu Asp Asp Asp Met Asn Leu Leu Asp Ile Phe Ile
130 135 140
Glu Met Glu Lys Arg Val Ile Leu Gly Glu Gly Lys Leu Asp Ile Leu
145 150 155 160
Lys Arg Val Cys Ala Gln Ile Asn Lys Ser Leu Leu Lys Ile Ile Asn
165 170 175
Asp Tyr Glu Glu Phe Ser Lys Glu Arg Ser Ser Ser Leu Glu Gly Ser
180 185 190
Pro Asp Glu Phe Ser Asn Gly Glu Glu Leu Cys Gly Val Met Thr Ile
195 200 205
Ser Asp Ser Pro Arg Glu Gln Asp Ser Glu Ser Gln Thr Leu Asp Lys
210 215 220
Val Tyr Gln Met Lys Ser Lys Pro Arg Gly Tyr Cys Leu Ile Ile Asn
225 230 235 240
Asn His Asn Phe Ala Lys Ala Arg Glu Lys Val Pro Lys Leu His Ser
245 250 255
Ile Arg Asp Arg Asn Gly Thr His Leu Asp Ala Gly Ala Leu Thr Thr
260 265 270
Thr Phe Glu Glu Leu His Phe Glu Ile Lys Pro His Asp Asp Cys Thr
275 280 285
Val Glu Gln Ile Tyr Glu Ile Leu Lys Ile Tyr Gln Leu Met Asp His
290 295 300
Ser Asn Met Asp Cys Phe Ile Cys Cys Ile Leu Ser His Gly Asp Lys
305 310 315 320
Gly Ile Ile Tyr Gly Thr Asp Gly Gln Glu Ala Pro Ile Tyr Glu Leu
325 330 335
Thr Ser Gln Phe Thr Gly Leu Lys Cys Pro Ser Leu Ala Gly Lys Pro
340 345 350
Lys Val Phe Phe Ile Gln Ala Ala Gln Gly Asp Asn Tyr Gln Lys Gly
355 360 365
Ile Pro Val Glu Thr Asp Ser Glu Glu Gln Pro Tyr Leu Glu Met Asp
370 375 380
Leu Ser Ser Pro Gln Thr Arg Tyr Ile Pro Asp Glu Ala Asp Phe Leu
385 390 395 400
Leu Gly Met Ala Thr Val Asn Asn Cys Val Ser Tyr Arg Asn Pro Ala
405 410 415
Glu Gly Thr Trp Tyr Ile Gln Ser Leu Cys Gln Ser Leu Arg Glu Arg
420 425 430
Cys Pro Arg Gly Asp Asp Ile Leu Thr Ile Leu Thr Glu Val Asn Tyr
435 440 445
Glu Val Ser Asn Lys Asp Asp Lys Lys Asn Met Gly Lys Gln Met Pro
450 455 460
Gln Pro Thr Phe Thr Leu Arg Lys Lys Leu Val Phe Pro Ser Asp
465 470 475
<210> 264
<211> 276
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 264
Met Asp Phe Ser Arg Asn Leu Tyr Asp Ile Gly Glu Gln Leu Asp Ser
1 5 10 15
Glu Asp Leu Ala Ser Leu Lys Phe Leu Ser Leu Asp Tyr Ile Pro Gln
20 25 30
Arg Lys Gln Glu Pro Ile Lys Asp Ala Leu Met Leu Phe Gln Arg Leu
35 40 45
Gln Glu Lys Arg Met Leu Glu Glu Ser Asn Leu Ser Phe Leu Lys Glu
50 55 60
Leu Leu Phe Arg Ile Asn Arg Leu Asp Leu Leu Ile Thr Tyr Leu Asn
65 70 75 80
Thr Arg Lys Glu Glu Met Glu Arg Glu Leu Gln Thr Pro Gly Arg Ala
85 90 95
Gln Ile Ser Ala Tyr Arg Val Met Leu Tyr Gln Ile Ser Glu Glu Val
100 105 110
Ser Arg Ser Glu Leu Arg Ser Phe Lys Phe Leu Leu Gln Glu Glu Ile
115 120 125
Ser Lys Cys Lys Leu Asp Asp Asp Met Asn Leu Leu Asp Ile Phe Ile
130 135 140
Glu Met Glu Lys Arg Val Ile Leu Gly Glu Gly Lys Leu Asp Ile Leu
145 150 155 160
Lys Arg Val Cys Ala Gln Ile Asn Lys Ser Leu Leu Lys Ile Ile Asn
165 170 175
Asp Tyr Glu Glu Phe Ser Lys Glu Arg Ser Ser Ser Leu Glu Gly Ser
180 185 190
Pro Asp Glu Phe Ser Asn Gly Glu Glu Leu Cys Gly Val Met Thr Ile
195 200 205
Ser Asp Ser Pro Arg Glu Gln Asp Ser Glu Ser Gln Thr Leu Asp Lys
210 215 220
Val Tyr Gln Met Lys Ser Lys Pro Arg Gly Tyr Cys Leu Ile Ile Asn
225 230 235 240
Asn His Asn Phe Ala Lys Ala Arg Glu Lys Val Pro Lys Leu His Ser
245 250 255
Ile Arg Asp Arg Asn Gly Thr His Leu Asp Ala Gly Thr Val Glu Pro
260 265 270
Lys Arg Glu Lys
275
<210> 265
<211> 416
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 265
Met Asp Glu Ala Asp Arg Arg Leu Leu Arg Arg Cys Arg Leu Arg Leu
1 5 10 15
Val Glu Glu Leu Gln Val Asp Gln Leu Trp Asp Ala Leu Leu Ser Arg
20 25 30
Glu Leu Phe Arg Pro His Met Ile Glu Asp Ile Gln Arg Ala Gly Ser
35 40 45
Gly Ser Arg Arg Asp Gln Ala Arg Gln Leu Ile Ile Asp Leu Glu Thr
50 55 60
Arg Gly Ser Gln Ala Leu Pro Leu Phe Ile Ser Cys Leu Glu Asp Thr
65 70 75 80
Gly Gln Asp Met Leu Ala Ser Phe Leu Arg Thr Asn Arg Gln Ala Ala
85 90 95
Lys Leu Ser Lys Pro Thr Leu Glu Asn Leu Thr Pro Val Val Leu Arg
100 105 110
Pro Glu Ile Arg Lys Pro Glu Val Leu Arg Pro Glu Thr Pro Arg Pro
115 120 125
Val Asp Ile Gly Ser Gly Gly Phe Gly Asp Val Gly Ala Leu Glu Ser
130 135 140
Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys
145 150 155 160
Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly
165 170 175
Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg
180 185 190
Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr
195 200 205
Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Gln Gln Asp His
210 215 220
Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln
225 230 235 240
Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys
245 250 255
Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys
260 265 270
Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Ser Gly
275 280 285
Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu
290 295 300
Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln
305 310 315 320
Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro
325 330 335
Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val
340 345 350
Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp
355 360 365
Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu
370 375 380
Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met
385 390 395 400
Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser
405 410 415
<210> 266
<211> 266
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 266
Met Asp Glu Ala Asp Arg Arg Leu Leu Arg Arg Cys Arg Leu Arg Leu
1 5 10 15
Val Glu Glu Leu Gln Val Asp Gln Leu Trp Asp Ala Leu Leu Ser Arg
20 25 30
Glu Leu Phe Arg Pro His Met Ile Glu Asp Ile Gln Arg Ala Gly Ser
35 40 45
Gly Ser Arg Arg Asp Gln Ala Arg Gln Leu Ile Ile Asp Leu Glu Thr
50 55 60
Arg Gly Ser Gln Ala Leu Pro Leu Phe Ile Ser Cys Leu Glu Asp Thr
65 70 75 80
Gly Gln Asp Met Leu Ala Ser Phe Leu Arg Thr Asn Arg Gln Ala Ala
85 90 95
Lys Leu Ser Lys Pro Thr Leu Glu Asn Leu Thr Pro Val Val Leu Arg
100 105 110
Pro Glu Ile Arg Lys Pro Glu Val Leu Arg Pro Glu Thr Pro Arg Pro
115 120 125
Val Asp Ile Gly Ser Gly Gly Phe Gly Asp Val Glu Gln Lys Asp His
130 135 140
Gly Phe Glu Val Ala Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser
145 150 155 160
Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe
165 170 175
Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe
180 185 190
Val Ser Tyr Ser Thr Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys
195 200 205
Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp
210 215 220
Ala His Ser Glu Asp Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala
225 230 235 240
Val Ser Val Lys Gly Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe
245 250 255
Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser
260 265
<210> 267
<211> 521
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 267
Met Lys Ser Gln Gly Gln His Trp Tyr Ser Ser Ser Asp Lys Asn Cys
1 5 10 15
Lys Val Ser Phe Arg Glu Lys Leu Leu Ile Ile Asp Ser Asn Leu Gly
20 25 30
Val Gln Asp Val Glu Asn Leu Lys Phe Leu Cys Ile Gly Leu Val Pro
35 40 45
Asn Lys Lys Leu Glu Lys Ser Ser Ser Ala Ser Asp Val Phe Glu His
50 55 60
Leu Leu Ala Glu Asp Leu Leu Ser Glu Glu Asp Pro Phe Phe Leu Ala
65 70 75 80
Glu Leu Leu Tyr Ile Ile Arg Gln Lys Lys Leu Leu Gln His Leu Asn
85 90 95
Cys Thr Lys Glu Glu Val Glu Arg Leu Leu Pro Thr Arg Gln Arg Val
100 105 110
Ser Leu Phe Arg Asn Leu Leu Tyr Glu Leu Ser Glu Gly Ile Asp Ser
115 120 125
Glu Asn Leu Lys Asp Met Ile Phe Leu Leu Lys Asp Ser Leu Pro Lys
130 135 140
Thr Glu Met Thr Ser Leu Ser Phe Leu Ala Phe Leu Glu Lys Gln Gly
145 150 155 160
Lys Ile Asp Glu Asp Asn Leu Thr Cys Leu Glu Asp Leu Cys Lys Thr
165 170 175
Val Val Pro Lys Leu Leu Arg Asn Ile Glu Lys Tyr Lys Arg Glu Lys
180 185 190
Ala Ile Gln Ile Val Thr Pro Pro Val Asp Lys Glu Ala Glu Ser Tyr
195 200 205
Gln Gly Glu Glu Glu Leu Val Ser Gln Thr Asp Val Lys Thr Phe Leu
210 215 220
Glu Ala Leu Pro Gln Glu Ser Trp Gln Asn Lys His Ala Gly Ser Asn
225 230 235 240
Gly Asn Arg Ala Thr Asn Gly Ala Pro Ser Leu Val Ser Arg Gly Met
245 250 255
Gln Gly Ala Ser Ala Asn Thr Leu Asn Ser Glu Thr Ser Thr Lys Arg
260 265 270
Ala Ala Val Tyr Arg Met Asn Arg Asn His Arg Gly Phe Cys Val Ile
275 280 285
Val Asn Asn His Ser Phe Thr Ser Leu Lys Asp Arg Gln Gly Thr His
290 295 300
Lys Asp Ala Glu Ile Leu Ser His Val Phe Gln Trp Leu Gly Phe Thr
305 310 315 320
Val His Ile His Asn Asn Val Thr Lys Val Glu Met Glu Met Val Leu
325 330 335
Gln Lys Gln Lys Cys Asn Pro Ala His Ala Asp Gly Asp Cys Phe Val
340 345 350
Phe Cys Ile Leu Thr His Gly Arg Phe Gly Ala Val Tyr Ser Ser Asp
355 360 365
Glu Ala Leu Ile Pro Ile Arg Glu Ile Met Ser His Phe Thr Ala Leu
370 375 380
Gln Cys Pro Arg Leu Ala Glu Lys Pro Lys Leu Phe Phe Ile Gln Ala
385 390 395 400
Cys Gln Gly Glu Glu Leu Gln Pro Ser Val Ser Ile Glu Ala Asp Ala
405 410 415
Leu Asn Pro Glu Gln Ala Pro Thr Ser Leu Gln Asp Ser Ile Pro Ala
420 425 430
Glu Ala Asp Phe Leu Leu Gly Leu Ala Thr Val Pro Gly Cys Val Ser
435 440 445
Phe Arg His Val Glu Glu Gly Ser Trp Tyr Ile Gln Ser Leu Cys Asn
450 455 460
His Leu Lys Lys Leu Val Pro Arg Met Leu Lys Phe Leu Glu Lys Thr
465 470 475 480
Met Glu Ile Arg Gly Arg Lys Arg Thr Val Trp Gly Ala Lys Gln Ile
485 490 495
Ser Ala Thr Ser Leu Pro Thr Ala Ile Ser Ala Gln Thr Pro Arg Pro
500 505 510
Pro Met Arg Arg Trp Ser Ser Val Ser
515 520
<210> 268
<211> 130
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 268
Met Asp Asp Phe Glu Ala Gly Ala Ala Ala Gly Ala Ala Pro Gly Glu
1 5 10 15
Glu Asp Leu Cys Ala Ala Phe Asn Val Ile Cys Asp Asn Val Gly Lys
20 25 30
Asp Trp Arg Arg Leu Ala Arg Gln Leu Lys Val Ser Asp Thr Lys Ile
35 40 45
Asp Ser Ile Glu Asp Arg Tyr Pro Arg Asn Leu Thr Glu Arg Val Arg
50 55 60
Glu Ser Leu Arg Ile Trp Lys Asn Thr Glu Lys Glu Asn Ala Thr Val
65 70 75 80
Ala His Leu Val Gly Ala Leu Arg Ser Cys Gln Met Asn Leu Val Ala
85 90 95
Asp Leu Val Gln Glu Val Gln Gln Ala Arg Asp Leu Gln Asn Arg Ser
100 105 110
Gly Ala Met Ser Pro Met Ser Trp Asn Ser Asp Ala Ser Thr Ser Glu
115 120 125
Ala Ser
130
<210> 269
<211> 435
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 269
Met Ser Ala Glu Val Ile His Gln Val Glu Glu Ala Leu Asp Thr Asp
1 5 10 15
Glu Lys Glu Met Leu Leu Phe Leu Cys Arg Asp Val Ala Ile Asp Val
20 25 30
Val Pro Pro Asn Val Arg Asp Leu Leu Asp Ile Leu Arg Glu Arg Gly
35 40 45
Lys Leu Ser Val Gly Asp Leu Ala Glu Leu Leu Tyr Arg Val Arg Arg
50 55 60
Phe Asp Leu Leu Lys Arg Ile Leu Lys Met Asp Arg Lys Ala Val Glu
65 70 75 80
Thr His Leu Leu Arg Asn Pro His Leu Val Ser Asp Tyr Arg Val Leu
85 90 95
Met Ala Glu Ile Gly Glu Asp Leu Asp Lys Ser Asp Val Ser Ser Leu
100 105 110
Ile Phe Leu Met Lys Asp Tyr Met Gly Arg Gly Lys Ile Ser Lys Glu
115 120 125
Lys Ser Phe Leu Asp Leu Val Val Glu Leu Glu Lys Leu Asn Leu Val
130 135 140
Ala Pro Asp Gln Leu Asp Leu Leu Glu Lys Cys Leu Lys Asn Ile His
145 150 155 160
Arg Ile Asp Leu Lys Thr Lys Ile Gln Lys Tyr Lys Gln Ser Val Gln
165 170 175
Gly Ala Gly Thr Ser Tyr Arg Asn Val Leu Gln Ala Ala Ile Gln Lys
180 185 190
Ser Leu Lys Asp Pro Ser Asn Asn Phe Arg Leu His Asn Gly Arg Ser
195 200 205
Lys Glu Gln Arg Leu Lys Glu Gln Leu Gly Ala Gln Gln Glu Pro Val
210 215 220
Lys Lys Ser Ile Gln Glu Ser Glu Ala Phe Leu Pro Gln Ser Ile Pro
225 230 235 240
Glu Glu Arg Tyr Lys Met Lys Ser Lys Pro Leu Gly Ile Cys Leu Ile
245 250 255
Ile Asp Cys Ile Gly Asn Glu Thr Glu Leu Leu Arg Asp Thr Phe Thr
260 265 270
Ser Leu Gly Tyr Glu Val Gln Lys Phe Leu His Leu Ser Met His Gly
275 280 285
Ile Ser Gln Ile Leu Gly Gln Phe Ala Cys Met Pro Glu His Arg Asp
290 295 300
Tyr Asp Ser Phe Val Cys Val Leu Val Ser Arg Gly Gly Ser Gln Ser
305 310 315 320
Val Tyr Gly Val Asp Gln Thr His Ser Gly Leu Pro Leu His His Ile
325 330 335
Arg Arg Met Phe Met Gly Asp Ser Cys Pro Tyr Leu Ala Gly Lys Pro
340 345 350
Lys Met Phe Phe Ile Gln Asn Tyr Val Val Ser Glu Gly Gln Leu Glu
355 360 365
Asp Ser Ser Leu Leu Glu Val Asp Gly Pro Ala Met Lys Asn Val Glu
370 375 380
Phe Lys Ala Gln Lys Arg Gly Leu Cys Thr Val His Arg Glu Ala Asp
385 390 395 400
Phe Phe Trp Ser Leu Cys Thr Ala Asp Met Ser Leu Leu Glu Gln Ser
405 410 415
His Ser Ser Pro Ser Leu Tyr Leu Gln Cys Leu Ser Gln Lys Leu Arg
420 425 430
Gln Glu Arg
435
<210> 270
<211> 239
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 270
Met Ala His Ala Gly Arg Thr Gly Tyr Asp Asn Arg Glu Ile Val Met
1 5 10 15
Lys Tyr Ile His Tyr Lys Leu Ser Gln Arg Gly Tyr Glu Trp Asp Ala
20 25 30
Gly Asp Val Gly Ala Ala Pro Pro Gly Ala Ala Pro Ala Pro Gly Ile
35 40 45
Phe Ser Ser Gln Pro Gly His Thr Pro His Pro Ala Ala Ser Arg Asp
50 55 60
Pro Val Ala Arg Thr Ser Pro Leu Gln Thr Pro Ala Ala Pro Gly Ala
65 70 75 80
Ala Ala Gly Pro Ala Leu Ser Pro Val Pro Pro Val Val His Leu Thr
85 90 95
Leu Arg Gln Ala Gly Asp Asp Phe Ser Arg Arg Tyr Arg Arg Asp Phe
100 105 110
Ala Glu Met Ser Ser Gln Leu His Leu Thr Pro Phe Thr Ala Arg Gly
115 120 125
Arg Phe Ala Thr Val Val Glu Glu Leu Phe Arg Asp Gly Val Asn Trp
130 135 140
Gly Arg Ile Val Ala Phe Phe Glu Phe Gly Gly Val Met Cys Val Glu
145 150 155 160
Ser Val Asn Arg Glu Met Ser Pro Leu Val Asp Asn Ile Ala Leu Trp
165 170 175
Met Thr Glu Tyr Leu Asn Arg His Leu His Thr Trp Ile Gln Asp Asn
180 185 190
Gly Gly Trp Asp Ala Phe Val Glu Leu Tyr Gly Pro Ser Met Arg Pro
195 200 205
Leu Phe Asp Phe Ser Trp Leu Ser Leu Lys Thr Leu Leu Ser Leu Ala
210 215 220
Leu Val Gly Ala Cys Ile Thr Leu Gly Ala Tyr Leu Gly His Lys
225 230 235
<210> 271
<211> 706
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 271
Met Ala Ser Pro Ala Asp Ser Cys Ile Gln Phe Thr Arg His Ala Ser
1 5 10 15
Asp Val Leu Leu Asn Leu Asn Arg Leu Arg Ser Arg Asp Ile Leu Thr
20 25 30
Asp Val Val Ile Val Val Ser Arg Glu Gln Phe Arg Ala His Lys Thr
35 40 45
Val Leu Met Ala Cys Ser Gly Leu Phe Tyr Ser Ile Phe Thr Asp Gln
50 55 60
Leu Lys Cys Asn Leu Ser Val Ile Asn Leu Asp Pro Glu Ile Asn Pro
65 70 75 80
Glu Gly Phe Cys Ile Leu Leu Asp Phe Met Tyr Thr Ser Arg Leu Asn
85 90 95
Leu Arg Glu Gly Asn Ile Met Ala Val Met Ala Thr Ala Met Tyr Leu
100 105 110
Gln Met Glu His Val Val Asp Thr Cys Arg Lys Phe Ile Lys Ala Ser
115 120 125
Glu Ala Glu Met Val Ser Ala Ile Lys Pro Pro Arg Glu Glu Phe Leu
130 135 140
Asn Ser Arg Met Leu Met Pro Gln Asp Ile Met Ala Tyr Arg Gly Arg
145 150 155 160
Glu Val Val Glu Asn Asn Leu Pro Leu Arg Ser Ala Pro Gly Cys Glu
165 170 175
Ser Arg Ala Phe Ala Pro Ser Leu Tyr Ser Gly Leu Ser Thr Pro Pro
180 185 190
Ala Ser Tyr Ser Met Tyr Ser His Leu Pro Val Ser Ser Leu Leu Phe
195 200 205
Ser Asp Glu Glu Phe Arg Asp Val Arg Met Pro Val Ala Asn Pro Phe
210 215 220
Pro Lys Glu Arg Ala Leu Pro Cys Asp Ser Ala Arg Pro Val Pro Gly
225 230 235 240
Glu Tyr Ser Arg Pro Thr Leu Glu Val Ser Pro Asn Val Cys His Ser
245 250 255
Asn Ile Tyr Ser Pro Lys Glu Thr Ile Pro Glu Glu Ala Arg Ser Asp
260 265 270
Met His Tyr Ser Val Ala Glu Gly Leu Lys Pro Ala Ala Pro Ser Ala
275 280 285
Arg Asn Ala Pro Tyr Phe Pro Cys Asp Lys Ala Ser Lys Glu Glu Glu
290 295 300
Arg Pro Ser Ser Glu Asp Glu Ile Ala Leu His Phe Glu Pro Pro Asn
305 310 315 320
Ala Pro Leu Asn Arg Lys Gly Leu Val Ser Pro Gln Ser Pro Gln Lys
325 330 335
Ser Asp Cys Gln Pro Asn Ser Pro Thr Glu Ser Cys Ser Ser Lys Asn
340 345 350
Ala Cys Ile Leu Gln Ala Ser Gly Ser Pro Pro Ala Lys Ser Pro Thr
355 360 365
Asp Pro Lys Ala Cys Asn Trp Lys Lys Tyr Lys Phe Ile Val Leu Asn
370 375 380
Ser Leu Asn Gln Asn Ala Lys Pro Glu Gly Pro Glu Gln Ala Glu Leu
385 390 395 400
Gly Arg Leu Ser Pro Arg Ala Tyr Thr Ala Pro Pro Ala Cys Gln Pro
405 410 415
Pro Met Glu Pro Glu Asn Leu Asp Leu Gln Ser Pro Thr Lys Leu Ser
420 425 430
Ala Ser Gly Glu Asp Ser Thr Ile Pro Gln Ala Ser Arg Leu Asn Asn
435 440 445
Ile Val Asn Arg Ser Met Thr Gly Ser Pro Arg Ser Ser Ser Glu Ser
450 455 460
His Ser Pro Leu Tyr Met His Pro Pro Lys Cys Thr Ser Cys Gly Ser
465 470 475 480
Gln Ser Pro Gln His Ala Glu Met Cys Leu His Thr Ala Gly Pro Thr
485 490 495
Phe Pro Glu Glu Met Gly Glu Thr Gln Ser Glu Tyr Ser Asp Ser Ser
500 505 510
Cys Glu Asn Gly Ala Phe Phe Cys Asn Glu Cys Asp Cys Arg Phe Ser
515 520 525
Glu Glu Ala Ser Leu Lys Arg His Thr Leu Gln Thr His Ser Asp Lys
530 535 540
Pro Tyr Lys Cys Asp Arg Cys Gln Ala Ser Phe Arg Tyr Lys Gly Asn
545 550 555 560
Leu Ala Ser His Lys Thr Val His Thr Gly Glu Lys Pro Tyr Arg Cys
565 570 575
Asn Ile Cys Gly Ala Gln Phe Asn Arg Pro Ala Asn Leu Lys Thr His
580 585 590
Thr Arg Ile His Ser Gly Glu Lys Pro Tyr Lys Cys Glu Thr Cys Gly
595 600 605
Ala Arg Phe Val Gln Val Ala His Leu Arg Ala His Val Leu Ile His
610 615 620
Thr Gly Glu Lys Pro Tyr Pro Cys Glu Ile Cys Gly Thr Arg Phe Arg
625 630 635 640
His Leu Gln Thr Leu Lys Ser His Leu Arg Ile His Thr Gly Glu Lys
645 650 655
Pro Tyr His Cys Glu Lys Cys Asn Leu His Phe Arg His Lys Ser Gln
660 665 670
Leu Arg Leu His Leu Arg Gln Lys His Gly Ala Ile Thr Asn Thr Lys
675 680 685
Val Gln Tyr Arg Val Ser Ala Thr Asp Leu Pro Pro Glu Leu Pro Lys
690 695 700
Ala Cys
705
<210> 272
<211> 970
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 272
Met Ala Ser Pro Ala Asp Ser Cys Ile Gln Phe Thr Arg His Ala Ser
1 5 10 15
Asp Val Leu Leu Asn Leu Asn Arg Leu Arg Ser Arg Asp Ile Leu Thr
20 25 30
Asp Val Val Ile Val Val Ser Arg Glu Gln Phe Arg Ala His Lys Thr
35 40 45
Val Leu Met Ala Cys Ser Gly Leu Phe Tyr Ser Ile Phe Thr Asp Gln
50 55 60
Leu Lys Cys Asn Leu Ser Val Ile Asn Leu Asp Pro Glu Ile Asn Pro
65 70 75 80
Glu Gly Phe Cys Ile Leu Leu Asp Phe Met Tyr Thr Ser Arg Leu Asn
85 90 95
Leu Arg Glu Gly Asn Ile Met Ala Val Met Ala Thr Ala Met Tyr Leu
100 105 110
Gln Met Glu His Val Val Asp Thr Cys Arg Lys Phe Ile Lys Ala Ser
115 120 125
Glu Ala Glu Met Val Ser Ala Ile Lys Pro Pro Arg Glu Glu Phe Leu
130 135 140
Asn Ser Arg Met Leu Met Pro Gln Asp Ile Met Ala Tyr Arg Gly Arg
145 150 155 160
Glu Val Val Glu Asn Asn Leu Pro Leu Arg Ser Ala Pro Gly Cys Glu
165 170 175
Ser Arg Ala Phe Ala Pro Ser Leu Tyr Ser Gly Leu Ser Thr Pro Pro
180 185 190
Ala Ser Tyr Ser Met Tyr Ser His Leu Pro Val Ser Ser Leu Leu Phe
195 200 205
Ser Asp Glu Glu Phe Arg Asp Val Arg Met Pro Val Ala Asn Pro Phe
210 215 220
Pro Lys Glu Arg Ala Leu Pro Cys Asp Ser Ala Arg Pro Val Pro Gly
225 230 235 240
Glu Tyr Ser Arg Pro Thr Leu Glu Val Ser Pro Asn Val Cys His Ser
245 250 255
Asn Ile Tyr Ser Pro Lys Glu Thr Ile Pro Glu Glu Ala Arg Ser Asp
260 265 270
Met His Tyr Ser Val Ala Glu Gly Leu Lys Pro Ala Ala Pro Ser Ala
275 280 285
Arg Asn Ala Pro Tyr Phe Pro Cys Asp Lys Ala Ser Lys Glu Glu Glu
290 295 300
Arg Pro Ser Ser Glu Asp Glu Ile Ala Leu His Phe Glu Pro Pro Asn
305 310 315 320
Ala Pro Leu Asn Arg Lys Gly Leu Val Ser Pro Gln Ser Pro Gln Lys
325 330 335
Ser Asp Cys Gln Pro Asn Ser Pro Thr Glu Ser Cys Ser Ser Lys Asn
340 345 350
Ala Cys Ile Leu Gln Ala Ser Gly Ser Pro Pro Ala Lys Ser Pro Thr
355 360 365
Asp Pro Lys Ala Cys Asn Trp Lys Lys Tyr Lys Phe Ile Val Leu Asn
370 375 380
Ser Leu Asn Gln Asn Ala Lys Pro Glu Gly Pro Glu Gln Ala Glu Leu
385 390 395 400
Gly Arg Leu Ser Pro Arg Ala Tyr Thr Ala Pro Pro Ala Cys Gln Pro
405 410 415
Pro Met Glu Pro Glu Asn Leu Asp Leu Gln Ser Pro Thr Lys Leu Ser
420 425 430
Ala Ser Gly Glu Asp Ser Thr Ile Pro Gln Ala Ser Arg Leu Asn Asn
435 440 445
Ile Val Asn Arg Ser Met Thr Gly Ser Pro Arg Ser Ser Ser Glu Ser
450 455 460
His Ser Pro Leu Tyr Met His Pro Pro Lys Cys Thr Ser Cys Gly Ser
465 470 475 480
Gln Ser Pro Gln His Ala Glu Met Cys Leu His Thr Ala Gly Pro Thr
485 490 495
Phe Pro Glu Glu Met Gly Glu Thr Gln Ser Glu Tyr Ser Asp Ser Ser
500 505 510
Cys Glu Asn Gly Ala Phe Phe Cys Asn Glu Cys Asp Cys Arg Phe Ser
515 520 525
Glu Glu Ala Ser Leu Lys Arg His Thr Leu Gln Thr His Ser Asp Lys
530 535 540
Pro Tyr Lys Cys Asp Arg Cys Gln Ala Ser Phe Arg Tyr Lys Gly Asn
545 550 555 560
Leu Ala Ser His Lys Thr Val His Thr Gly Glu Lys Pro Tyr Arg Cys
565 570 575
Asn Ile Cys Gly Ala Gln Phe Asn Arg Pro Ala Asn Leu Lys Thr His
580 585 590
Thr Arg Ile His Ser Gly Glu Lys Pro Tyr Lys Cys Glu Thr Cys Gly
595 600 605
Ala Arg Phe Val Gln Val Ala His Leu Arg Ala His Val Leu Ile His
610 615 620
Thr Gly Glu Lys Pro Tyr Pro Cys Glu Ile Cys Gly Thr Arg Phe Arg
625 630 635 640
His Leu Gln Thr Leu Lys Ser His Leu Arg Ile His Thr Gly Glu Lys
645 650 655
Pro Tyr His Cys Glu Lys Cys Asn Leu His Phe Arg His Lys Ser Gln
660 665 670
Leu Arg Leu His Leu Arg Gln Lys His Gly Ala Ile Thr Asn Thr Lys
675 680 685
Val Gln Tyr Arg Val Ser Ala Thr Asp Leu Pro Pro Glu Leu Pro Lys
690 695 700
Ala Cys Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys
705 710 715 720
Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro Met Ala His Ala Gly
725 730 735
Arg Thr Gly Tyr Asp Asn Arg Glu Ile Val Met Lys Tyr Ile His Tyr
740 745 750
Lys Leu Ser Gln Arg Gly Tyr Glu Trp Asp Ala Gly Asp Val Gly Ala
755 760 765
Ala Pro Pro Gly Ala Ala Pro Ala Pro Gly Ile Phe Ser Ser Gln Pro
770 775 780
Gly His Thr Pro His Pro Ala Ala Ser Arg Asp Pro Val Ala Arg Thr
785 790 795 800
Ser Pro Leu Gln Thr Pro Ala Ala Pro Gly Ala Ala Ala Gly Pro Ala
805 810 815
Leu Ser Pro Val Pro Pro Val Val His Leu Thr Leu Arg Gln Ala Gly
820 825 830
Asp Asp Phe Ser Arg Arg Tyr Arg Arg Asp Phe Ala Glu Met Ser Ser
835 840 845
Gln Leu His Leu Thr Pro Phe Thr Ala Arg Gly Arg Phe Ala Thr Val
850 855 860
Val Glu Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile Val Ala
865 870 875 880
Phe Phe Glu Phe Gly Gly Val Met Cys Val Glu Ser Val Asn Arg Glu
885 890 895
Met Ser Pro Leu Val Asp Asn Ile Ala Leu Trp Met Thr Glu Tyr Leu
900 905 910
Asn Arg His Leu His Thr Trp Ile Gln Asp Asn Gly Gly Trp Asp Ala
915 920 925
Phe Val Glu Leu Tyr Gly Pro Ser Met Arg Pro Leu Phe Asp Phe Ser
930 935 940
Trp Leu Ser Leu Lys Thr Leu Leu Ser Leu Ala Leu Val Gly Ala Cys
945 950 955 960
Ile Thr Leu Gly Ala Tyr Leu Gly His Lys
965 970
<210> 273
<211> 376
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 273
Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala
1 5 10 15
Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg
20 25 30
Arg Gln Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr
35 40 45
Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr
50 55 60
Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr
65 70 75 80
Val Pro Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr
85 90 95
Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile
100 105 110
Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met
115 120 125
Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Ile Gly
130 135 140
Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile
145 150 155 160
Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg
165 170 175
Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala
180 185 190
Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu
195 200 205
Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly
210 215 220
Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly
225 230 235 240
Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Arg
245 250 255
Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala
260 265 270
Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu
275 280 285
Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu
290 295 300
Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg
305 310 315 320
Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys
325 330 335
Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val
340 345 350
Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe
355 360 365
Ala Arg Glu Met Gly Glu Ala Asn
370 375
<210> 274
<211> 232
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 274
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp
165 170 175
Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp Val Lys Arg
180 185 190
Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro
195 200 205
Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu
210 215 220
Glu Glu Glu Gly Gly Cys Glu Leu
225 230
<210> 275
<211> 273
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 275
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp
165 170 175
Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp Val Arg Ser
180 185 190
Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Pro Pro Arg
195 200 205
Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg
210 215 220
Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr
225 230 235 240
Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu
245 250 255
Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu
260 265 270
Leu
<210> 276
<211> 298
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 276
Met Ala Pro Pro Pro Ala Arg Val His Leu Gly Ala Phe Leu Ala Val
1 5 10 15
Thr Pro Asn Pro Gly Ser Ala Ala Ser Gly Thr Glu Ala Ala Ala Ala
20 25 30
Thr Pro Ser Lys Val Trp Gly Ser Ser Ala Gly Arg Ile Glu Pro Arg
35 40 45
Gly Gly Gly Arg Gly Ala Leu Pro Thr Ser Met Gly Gln His Gly Pro
50 55 60
Ser Ala Arg Ala Arg Ala Gly Arg Ala Pro Gly Pro Arg Pro Ala Arg
65 70 75 80
Glu Ala Ser Pro Arg Leu Arg Val His Lys Thr Phe Lys Phe Val Val
85 90 95
Val Gly Val Leu Leu Gln Val Val Pro Ser Ser Ala Ala Thr Ile Lys
100 105 110
Leu His Asp Gln Ser Ile Gly Thr Gln Gln Trp Glu His Ser Pro Leu
115 120 125
Gly Glu Leu Cys Pro Pro Gly Ser His Arg Ser Glu His Pro Gly Ala
130 135 140
Cys Asn Arg Cys Thr Glu Gly Val Gly Tyr Thr Asn Ala Ser Asn Asn
145 150 155 160
Leu Phe Ala Cys Leu Pro Cys Thr Ala Cys Lys Ser Asp Glu Glu Glu
165 170 175
Arg Ser Pro Cys Thr Thr Thr Arg Asn Thr Ala Cys Gln Cys Lys Pro
180 185 190
Gly Thr Phe Arg Asn Asp Asn Ser Ala Glu Met Cys Arg Lys Cys Ser
195 200 205
Arg Gly Cys Pro Arg Gly Met Val Lys Val Lys Asp Cys Thr Pro Trp
210 215 220
Ser Asp Ile Glu Cys Val His Lys Glu Ser Gly Asn Gly His Asn Leu
225 230 235 240
Gly Trp Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp Val
245 250 255
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
260 265 270
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
275 280 285
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
290 295
<210> 277
<211> 264
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 277
Met Glu Gln Arg Pro Arg Gly Cys Ala Ala Val Ala Ala Ala Leu Leu
1 5 10 15
Leu Val Leu Leu Gly Ala Arg Ala Gln Gly Gly Thr Arg Ser Pro Arg
20 25 30
Cys Asp Cys Ala Gly Asp Phe His Lys Lys Ile Gly Leu Phe Cys Cys
35 40 45
Arg Gly Cys Pro Ala Gly His Tyr Leu Lys Ala Pro Cys Thr Glu Pro
50 55 60
Cys Gly Asn Ser Thr Cys Leu Val Cys Pro Gln Asp Thr Phe Leu Ala
65 70 75 80
Trp Glu Asn His His Asn Ser Glu Cys Ala Arg Cys Gln Ala Cys Asp
85 90 95
Glu Gln Ala Ser Gln Val Ala Leu Glu Asn Cys Ser Ala Val Ala Asp
100 105 110
Thr Arg Cys Gly Cys Lys Pro Gly Trp Phe Val Glu Cys Gln Val Ser
115 120 125
Gln Cys Val Ser Ser Ser Pro Phe Tyr Cys Gln Pro Cys Leu Asp Cys
130 135 140
Gly Ala Leu His Arg His Thr Arg Leu Leu Cys Ser Arg Arg Asp Thr
145 150 155 160
Asp Cys Gly Thr Cys Leu Pro Gly Phe Tyr Glu His Gly Asp Gly Cys
165 170 175
Val Ser Cys Pro Thr Ser Thr Leu Gly Ser Cys Pro Glu Arg Cys Ala
180 185 190
Ala Val Cys Gly Trp Arg Gln Met Phe Trp Val Gln Val Leu Leu Ala
195 200 205
Gly Leu Val Val Pro Leu Leu Leu Gly Ala Thr Leu Thr Tyr Lys Arg
210 215 220
Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro
225 230 235 240
Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu
245 250 255
Glu Glu Glu Gly Gly Cys Glu Leu
260
<210> 278
<211> 412
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 278
Met Gly Thr Ser Pro Ser Ser Ser Thr Ala Leu Ala Ser Cys Ser Arg
1 5 10 15
Ile Ala Arg Arg Ala Thr Ala Thr Met Ile Ala Gly Ser Leu Leu Leu
20 25 30
Leu Gly Phe Leu Ser Thr Thr Thr Ala Gln Pro Glu Gln Lys Ala Ser
35 40 45
Asn Leu Ile Gly Thr Tyr Arg His Val Asp Arg Ala Thr Gly Gln Val
50 55 60
Leu Thr Cys Asp Lys Cys Pro Ala Gly Thr Tyr Val Ser Glu His Cys
65 70 75 80
Thr Asn Thr Ser Leu Arg Val Cys Ser Ser Cys Pro Val Gly Thr Phe
85 90 95
Thr Arg His Glu Asn Gly Ile Glu Lys Cys His Asp Cys Ser Gln Pro
100 105 110
Cys Pro Trp Pro Met Ile Glu Lys Leu Pro Cys Ala Ala Leu Thr Asp
115 120 125
Arg Glu Cys Thr Cys Pro Pro Gly Met Phe Gln Ser Asn Ala Thr Cys
130 135 140
Ala Pro His Thr Val Cys Pro Val Gly Trp Gly Val Arg Lys Lys Gly
145 150 155 160
Thr Glu Thr Glu Asp Val Arg Cys Lys Gln Cys Ala Arg Gly Thr Phe
165 170 175
Ser Asp Val Pro Ser Ser Val Met Lys Cys Lys Ala Tyr Thr Asp Cys
180 185 190
Leu Ser Gln Asn Leu Val Val Ile Lys Pro Gly Thr Lys Glu Thr Asp
195 200 205
Asn Val Cys Gly Thr Leu Pro Ser Phe Ser Ser Ser Thr Ser Pro Ser
210 215 220
Pro Gly Thr Ala Ile Phe Pro Arg Pro Glu His Met Glu Thr His Glu
225 230 235 240
Val Pro Ser Ser Thr Tyr Val Pro Lys Gly Met Asn Ser Thr Glu Ser
245 250 255
Asn Ser Ser Ala Ser Val Arg Pro Lys Val Leu Ser Ser Ile Gln Glu
260 265 270
Gly Thr Val Pro Asp Asn Thr Ser Ser Ala Arg Gly Lys Glu Asp Val
275 280 285
Asn Lys Thr Leu Pro Asn Leu Gln Val Val Asn His Gln Gln Gly Pro
290 295 300
His His Arg His Ile Leu Lys Leu Leu Pro Ser Met Glu Ala Thr Gly
305 310 315 320
Gly Glu Lys Ser Ser Thr Pro Ile Lys Gly Pro Lys Arg Gly His Pro
325 330 335
Arg Gln Asn Leu His Lys His Phe Asp Ile Asn Glu His Leu Pro Trp
340 345 350
Met Ile Val Leu Phe Leu Leu Leu Val Leu Val Val Ile Val Val Cys
355 360 365
Ser Ile Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro
370 375 380
Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys
385 390 395 400
Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
405 410
<210> 279
<211> 305
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 279
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Phe Leu Phe
165 170 175
Val Leu Leu Gly Val Gly Ser Met Gly Val Ala Ala Ile Val Trp Gly
180 185 190
Ala Trp Phe Trp Gly Arg Arg Ser Cys Gln Gln Arg Asp Ser Gly Asn
195 200 205
Ser Pro Gly Asn Ala Phe Tyr Ser Asn Val Leu Tyr Arg Pro Arg Gly
210 215 220
Ala Pro Lys Lys Ser Glu Asp Cys Ser Gly Glu Gly Lys Asp Gln Arg
225 230 235 240
Gly Gln Ser Ile Tyr Ser Thr Ser Phe Pro Gln Pro Ala Pro Arg Gln
245 250 255
Pro His Leu Ala Ser Arg Pro Cys Pro Ser Pro Arg Pro Cys Pro Ser
260 265 270
Pro Arg Pro Gly His Pro Val Ser Met Val Arg Val Ser Pro Arg Pro
275 280 285
Ser Pro Thr Gln Gln Pro Arg Pro Lys Gly Phe Pro Lys Val Gly Glu
290 295 300
Glu
305
<210> 280
<211> 232
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 280
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Phe Trp Leu
165 170 175
Pro Ile Gly Cys Ala Ala Phe Val Val Val Cys Ile Leu Gly Cys Ile
180 185 190
Leu Ile Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp
195 200 205
Pro Asn Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys
210 215 220
Ser Arg Leu Thr Asp Val Thr Leu
225 230
<210> 281
<211> 242
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 281
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Leu Val
165 170 175
Ile Phe Ser Gly Met Phe Leu Val Phe Thr Leu Ala Gly Ala Leu Phe
180 185 190
Leu His Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val
195 200 205
Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly
210 215 220
Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys
225 230 235 240
Ser Pro
<210> 282
<211> 236
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 282
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Val Ala Ala
165 170 175
Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly Pro Leu Ala Ile
180 185 190
Leu Leu Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp
195 200 205
Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu
210 215 220
Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile
225 230 235
<210> 283
<211> 242
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 283
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Ile Ser
165 170 175
Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe
180 185 190
Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu
195 200 205
Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu
210 215 220
Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys
225 230 235 240
Glu Leu
<210> 284
<211> 391
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 284
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Met Phe Trp
165 170 175
Val Gln Val Leu Leu Ala Gly Leu Val Val Pro Leu Leu Leu Gly Ala
180 185 190
Thr Leu Thr Tyr Thr Tyr Arg His Cys Trp Pro His Lys Pro Leu Val
195 200 205
Thr Ala Asp Glu Ala Gly Met Glu Ala Leu Thr Pro Pro Pro Ala Thr
210 215 220
His Leu Ser Pro Leu Asp Ser Ala His Thr Leu Leu Ala Pro Pro Asp
225 230 235 240
Ser Ser Glu Lys Ile Cys Thr Val Gln Leu Val Gly Asn Ser Trp Thr
245 250 255
Pro Gly Tyr Pro Glu Thr Gln Glu Ala Leu Cys Pro Gln Val Thr Trp
260 265 270
Ser Trp Asp Gln Leu Pro Ser Arg Ala Leu Gly Pro Ala Ala Ala Pro
275 280 285
Thr Leu Ser Pro Glu Ser Pro Ala Gly Ser Pro Ala Met Met Leu Gln
290 295 300
Pro Gly Pro Gln Leu Tyr Asp Val Met Asp Ala Val Pro Ala Arg Arg
305 310 315 320
Trp Lys Glu Phe Val Arg Thr Leu Gly Leu Arg Glu Ala Glu Ile Glu
325 330 335
Ala Val Glu Val Glu Ile Gly Arg Phe Arg Asp Gln Gln Tyr Glu Met
340 345 350
Leu Lys Arg Trp Arg Gln Gln Gln Pro Ala Gly Leu Gly Ala Val Tyr
355 360 365
Ala Ala Leu Glu Arg Met Gly Leu Asp Gly Cys Val Glu Asp Leu Arg
370 375 380
Ser Arg Leu Gln Arg Gly Pro
385 390
<210> 285
<211> 412
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 285
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Phe Met
165 170 175
Tyr Leu Leu Thr Val Phe Leu Ile Thr Gln Met Ile Gly Ser Ala Leu
180 185 190
Phe Ala Val Tyr Leu His Arg Arg Leu Asp Lys Ile Glu Asp Glu Arg
195 200 205
Asn Leu His Glu Asp Phe Val Phe Met Lys Thr Ile Gln Arg Cys Asn
210 215 220
Thr Gly Glu Arg Ser Leu Ser Leu Leu Asn Cys Glu Glu Ile Lys Ser
225 230 235 240
Gln Phe Glu Gly Phe Val Lys Asp Ile Met Leu Asn Lys Glu Glu Thr
245 250 255
Lys Lys Glu Asn Ser Phe Glu Met Gln Lys Gly Asp Gln Asn Pro Gln
260 265 270
Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val
275 280 285
Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val
290 295 300
Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr
305 310 315 320
Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser
325 330 335
Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe
340 345 350
Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro
355 360 365
Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro
370 375 380
Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His
385 390 395 400
Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu
405 410
<210> 286
<211> 376
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 286
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Val Gly Leu
165 170 175
Gly Leu Leu Leu Leu Leu Met Gly Ala Gly Leu Ala Val Gln Gly Trp
180 185 190
Phe Leu Leu Gln Leu His Trp Arg Leu Gly Glu Met Val Thr Arg Leu
195 200 205
Pro Asp Gly Pro Ala Gly Ser Trp Glu Gln Leu Ile Gln Glu Arg Arg
210 215 220
Ser His Glu Val Asn Pro Ala Ala His Leu Thr Gly Ala Asn Ser Ser
225 230 235 240
Leu Thr Gly Ser Gly Gly Pro Leu Leu Trp Glu Thr Gln Leu Gly Leu
245 250 255
Ala Phe Leu Arg Gly Leu Ser Tyr His Asp Gly Ala Leu Val Val Thr
260 265 270
Lys Ala Gly Tyr Tyr Tyr Ile Tyr Ser Lys Val Gln Leu Gly Gly Val
275 280 285
Gly Cys Pro Leu Gly Leu Ala Ser Thr Ile Thr His Gly Leu Tyr Lys
290 295 300
Arg Thr Pro Arg Tyr Pro Glu Glu Leu Glu Leu Leu Val Ser Gln Gln
305 310 315 320
Ser Pro Cys Gly Arg Ala Thr Ser Ser Ser Arg Val Trp Trp Asp Ser
325 330 335
Ser Phe Leu Gly Gly Val Val His Leu Glu Ala Gly Glu Lys Val Val
340 345 350
Val Arg Val Leu Asp Glu Arg Leu Val Arg Leu Arg Asp Gly Thr Arg
355 360 365
Ser Tyr Phe Gly Ala Phe Met Val
370 375
<210> 287
<211> 254
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 287
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Trp Trp Phe
165 170 175
Leu Ser Gly Ser Leu Val Ile Val Ile Val Cys Ser Thr Val Gly Leu
180 185 190
Ile Ile Cys Val Lys Arg Arg Lys Pro Arg Gly Asp Val Val Lys Val
195 200 205
Ile Val Ser Val Gln Arg Lys Arg Gln Glu Ala Glu Gly Glu Ala Thr
210 215 220
Val Ile Glu Ala Leu Gln Ala Pro Pro Asp Val Thr Thr Val Ala Val
225 230 235 240
Glu Glu Thr Ile Pro Ser Phe Thr Gly Arg Ser Pro Asn His
245 250
<210> 288
<211> 389
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 288
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Pro Val Leu
165 170 175
Asp Ala Gly Pro Val Leu Phe Trp Val Ile Leu Val Leu Val Val Val
180 185 190
Val Gly Ser Ser Ala Phe Leu Leu Cys His Arg Arg Ala Cys Arg Lys
195 200 205
Arg Ile Arg Gln Lys Leu His Leu Cys Tyr Pro Val Gln Thr Ser Gln
210 215 220
Pro Lys Leu Glu Leu Val Asp Ser Arg Pro Arg Arg Ser Ser Thr Gln
225 230 235 240
Leu Arg Ser Gly Ala Ser Val Thr Glu Pro Val Ala Glu Glu Arg Gly
245 250 255
Leu Met Ser Gln Pro Leu Met Glu Thr Cys His Ser Val Gly Ala Ala
260 265 270
Tyr Leu Glu Ser Leu Pro Leu Gln Asp Ala Ser Pro Ala Gly Gly Pro
275 280 285
Ser Ser Pro Arg Asp Leu Pro Glu Pro Arg Val Ser Thr Glu His Thr
290 295 300
Asn Asn Lys Ile Glu Lys Ile Tyr Ile Met Lys Ala Asp Thr Val Ile
305 310 315 320
Val Gly Thr Val Lys Ala Glu Leu Pro Glu Gly Arg Gly Leu Ala Gly
325 330 335
Pro Ala Glu Pro Glu Leu Glu Glu Glu Leu Glu Ala Asp His Thr Pro
340 345 350
His Tyr Pro Glu Gln Glu Thr Glu Pro Pro Leu Gly Ser Cys Ser Asp
355 360 365
Val Met Leu Ser Val Glu Glu Glu Gly Lys Glu Asp Pro Leu Pro Thr
370 375 380
Ala Ala Ser Gly Lys
385
<210> 289
<211> 252
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 289
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Leu Gly Trp
165 170 175
Leu Thr Val Val Leu Leu Ala Val Ala Ala Cys Val Leu Leu Leu Thr
180 185 190
Ser Ala Gln Leu Gly Leu His Ile Trp Gln Leu Arg Ser Gln Cys Met
195 200 205
Trp Pro Arg Glu Thr Gln Leu Leu Leu Glu Val Pro Pro Ser Thr Glu
210 215 220
Asp Ala Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg Gly Glu Arg Ser
225 230 235 240
Ala Glu Glu Lys Gly Arg Leu Gly Asp Leu Trp Val
245 250
<210> 290
<211> 338
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 290
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Pro Phe Phe
165 170 175
Phe Cys Cys Phe Ile Ala Val Ala Met Gly Ile Arg Phe Ile Ile Met
180 185 190
Val Ala Ile Trp Ser Ala Val Phe Leu Asn Ser Leu Phe Asn Gln Glu
195 200 205
Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro Cys Pro Lys Asn
210 215 220
Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe Asp Glu Ser Lys
225 230 235 240
Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln Asn Ala Ser Leu
245 250 255
Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu Lys Leu Val Lys
260 265 270
Ser Tyr His Trp Met Gly Leu Val His Ile Pro Thr Asn Gly Ser Trp
275 280 285
Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu Leu Thr Ile Ile
290 295 300
Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser Ser Phe Lys Gly
305 310 315 320
Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile Cys Met Gln Arg
325 330 335
Thr Val
<210> 291
<211> 255
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 291
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Gly Gly Thr
165 170 175
Val Leu Leu Leu Leu Phe Val Ile Ser Ile Thr Thr Ile Ile Val Ile
180 185 190
Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu
195 200 205
Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser
210 215 220
Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile
225 230 235 240
Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val
245 250 255
<210> 292
<211> 271
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 292
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Trp Ala Val
165 170 175
Tyr Ala Gly Leu Leu Gly Gly Val Ile Met Ile Leu Ile Met Val Val
180 185 190
Ile Leu Gln Leu Arg Arg Arg Gly Lys Thr Asn His Tyr Gln Thr Thr
195 200 205
Val Glu Lys Lys Ser Leu Thr Ile Tyr Ala Gln Val Gln Lys Pro Gly
210 215 220
Pro Leu Gln Lys Lys Leu Asp Ser Phe Pro Ala Gln Asp Pro Cys Thr
225 230 235 240
Thr Ile Tyr Val Ala Ala Thr Glu Pro Val Pro Glu Ser Val Gln Glu
245 250 255
Thr Asn Ser Ile Thr Val Tyr Ala Ser Val Thr Leu Pro Glu Ser
260 265 270
<210> 293
<211> 242
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 293
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Tyr Ala
165 170 175
Gly Val Cys Ile Ser Val Leu Val Leu Leu Ala Leu Leu Gly Val Ile
180 185 190
Ile Ala Lys Lys Tyr Phe Phe Lys Lys Glu Val Gln Gln Leu Ser Val
195 200 205
Ser Phe Ser Ser Leu Gln Ile Lys Ala Leu Gln Asn Ala Val Glu Lys
210 215 220
Glu Val Gln Ala Glu Asp Asn Ile Tyr Ile Glu Asn Ser Leu Tyr Ala
225 230 235 240
Thr Asp
<210> 294
<211> 294
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 294
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Trp Leu
165 170 175
Ile Val Gly Ile Cys Ile Ala Leu Phe Ala Leu Pro Phe Val Ile Tyr
180 185 190
Ala Ala Lys Val Phe Leu Arg Cys Ile Asn Tyr Val Phe Phe Pro Ser
195 200 205
Leu Lys Pro Ser Ser Ser Ile Asp Glu Tyr Phe Ser Glu Gln Pro Leu
210 215 220
Lys Asn Leu Leu Leu Ser Thr Ser Glu Glu Gln Ile Glu Lys Cys Phe
225 230 235 240
Ile Ile Glu Asn Ile Ser Thr Ile Ala Thr Val Glu Glu Thr Asn Gln
245 250 255
Thr Asp Glu Asp His Lys Lys Tyr Ser Ser Gln Thr Ser Gln Asp Ser
260 265 270
Gly Asn Tyr Ser Asn Glu Asp Glu Ser Glu Ser Lys Thr Ser Glu Glu
275 280 285
Leu Gln Gln Asp Phe Val
290
<210> 295
<211> 445
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 295
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Gly Gly
165 170 175
Ile Ile Thr Val Phe Leu Ile Ala Leu Val Leu Thr Ser Thr Ile Val
180 185 190
Thr Leu Lys Trp Ile Gly Tyr Ile Cys Leu Arg Asn Ser Leu Pro Lys
195 200 205
Val Leu Asn Phe His Asn Phe Leu Ala Trp Pro Phe Pro Asn Leu Pro
210 215 220
Pro Leu Glu Ala Met Asp Met Val Glu Val Ile Tyr Ile Asn Arg Lys
225 230 235 240
Lys Lys Val Trp Asp Tyr Asn Tyr Asp Asp Glu Ser Asp Ser Asp Thr
245 250 255
Glu Ala Ala Pro Arg Thr Ser Gly Gly Gly Tyr Thr Met His Gly Leu
260 265 270
Thr Val Arg Pro Leu Gly Gln Ala Ser Ala Thr Ser Thr Glu Ser Gln
275 280 285
Leu Ile Asp Pro Glu Ser Glu Glu Glu Pro Asp Leu Pro Glu Val Asp
290 295 300
Val Glu Leu Pro Thr Met Pro Lys Asp Ser Pro Gln Gln Leu Glu Leu
305 310 315 320
Leu Ser Gly Pro Cys Glu Arg Arg Lys Ser Pro Leu Gln Asp Pro Phe
325 330 335
Pro Glu Glu Asp Tyr Ser Ser Thr Glu Gly Ser Gly Gly Arg Ile Thr
340 345 350
Phe Asn Val Asp Leu Asn Ser Val Phe Leu Arg Val Leu Asp Asp Glu
355 360 365
Asp Ser Asp Asp Leu Glu Ala Pro Leu Met Leu Ser Ser His Leu Glu
370 375 380
Glu Met Val Asp Pro Glu Asp Pro Asp Asn Val Gln Ser Asn His Leu
385 390 395 400
Leu Ala Ser Gly Glu Gly Thr Gln Pro Thr Phe Pro Ser Pro Ser Ser
405 410 415
Glu Gly Leu Trp Ser Glu Asp Ala Pro Ser Asp Gln Ser Asp Thr Ser
420 425 430
Glu Ser Asp Val Asp Leu Gly Asp Gly Tyr Ile Met Arg
435 440 445
<210> 296
<211> 553
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 296
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Tyr Ala
165 170 175
Gly Val Cys Ile Ser Val Leu Val Leu Leu Ala Leu Leu Gly Val Ile
180 185 190
Ile Ala Lys Val Phe Leu Arg Cys Ile Asn Tyr Val Phe Phe Pro Ser
195 200 205
Leu Lys Pro Ser Ser Ser Ile Asp Glu Tyr Phe Ser Glu Gln Pro Leu
210 215 220
Lys Asn Leu Leu Leu Ser Thr Ser Glu Glu Gln Ile Glu Lys Cys Phe
225 230 235 240
Ile Ile Glu Asn Ile Ser Thr Ile Ala Thr Val Glu Glu Thr Asn Gln
245 250 255
Thr Asp Glu Asp His Lys Lys Tyr Ser Ser Gln Thr Ser Gln Asp Ser
260 265 270
Gly Asn Tyr Ser Asn Glu Asp Glu Ser Glu Ser Lys Thr Ser Glu Glu
275 280 285
Leu Gln Gln Asp Phe Val Gly Gly Gly Ser Gly Gly Gly Ser Lys Trp
290 295 300
Ile Gly Tyr Ile Cys Leu Arg Asn Ser Leu Pro Lys Val Leu Asn Phe
305 310 315 320
His Asn Phe Leu Ala Trp Pro Phe Pro Asn Leu Pro Pro Leu Glu Ala
325 330 335
Met Asp Met Val Glu Val Ile Tyr Ile Asn Arg Lys Lys Lys Val Trp
340 345 350
Asp Tyr Asn Tyr Asp Asp Glu Ser Asp Ser Asp Thr Glu Ala Ala Pro
355 360 365
Arg Thr Ser Gly Gly Gly Tyr Thr Met His Gly Leu Thr Val Arg Pro
370 375 380
Leu Gly Gln Ala Ser Ala Thr Ser Thr Glu Ser Gln Leu Ile Asp Pro
385 390 395 400
Glu Ser Glu Glu Glu Pro Asp Leu Pro Glu Val Asp Val Glu Leu Pro
405 410 415
Thr Met Pro Lys Asp Ser Pro Gln Gln Leu Glu Leu Leu Ser Gly Pro
420 425 430
Cys Glu Arg Arg Lys Ser Pro Leu Gln Asp Pro Phe Pro Glu Glu Asp
435 440 445
Tyr Ser Ser Thr Glu Gly Ser Gly Gly Arg Ile Thr Phe Asn Val Asp
450 455 460
Leu Asn Ser Val Phe Leu Arg Val Leu Asp Asp Glu Asp Ser Asp Asp
465 470 475 480
Leu Glu Ala Pro Leu Met Leu Ser Ser His Leu Glu Glu Met Val Asp
485 490 495
Pro Glu Asp Pro Asp Asn Val Gln Ser Asn His Leu Leu Ala Ser Gly
500 505 510
Glu Gly Thr Gln Pro Thr Phe Pro Ser Pro Ser Ser Glu Gly Leu Trp
515 520 525
Ser Glu Asp Ala Pro Ser Asp Gln Ser Asp Thr Ser Glu Ser Asp Val
530 535 540
Asp Leu Gly Asp Gly Tyr Ile Met Arg
545 550
<210> 297
<211> 512
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 297
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Ile Tyr Ala
165 170 175
Gly Val Cys Ile Ser Val Leu Val Leu Leu Ala Leu Leu Gly Val Ile
180 185 190
Ile Ala Asn Arg Ala Ala Arg His Leu Cys Pro Pro Leu Pro Thr Pro
195 200 205
Cys Ala Ser Ser Ala Ile Glu Phe Pro Gly Gly Lys Glu Thr Trp Gln
210 215 220
Trp Ile Asn Pro Val Asp Phe Gln Glu Glu Ala Ser Leu Gln Glu Ala
225 230 235 240
Leu Val Val Glu Met Ser Trp Asp Lys Gly Glu Arg Thr Glu Pro Leu
245 250 255
Glu Lys Thr Glu Leu Pro Glu Gly Ala Pro Glu Leu Ala Leu Asp Thr
260 265 270
Glu Leu Ser Leu Glu Asp Gly Asp Arg Cys Lys Ala Lys Met Gly Gly
275 280 285
Ser Gly Gly Gly Ser His Tyr Phe Gln Gln Lys Val Phe Val Leu Leu
290 295 300
Ala Ala Leu Arg Pro Gln Trp Cys Ser Arg Glu Ile Pro Asp Pro Ala
305 310 315 320
Asn Ser Thr Cys Ala Lys Lys Tyr Pro Ile Ala Glu Glu Lys Thr Gln
325 330 335
Leu Pro Leu Asp Arg Leu Leu Ile Asp Trp Pro Thr Pro Glu Asp Pro
340 345 350
Glu Pro Leu Val Ile Ser Glu Val Leu His Gln Val Thr Pro Val Phe
355 360 365
Arg His Pro Pro Cys Ser Asn Trp Pro Gln Arg Glu Lys Gly Ile Gln
370 375 380
Gly His Gln Ala Ser Glu Lys Asp Met Met His Ser Ala Ser Ser Pro
385 390 395 400
Pro Pro Pro Arg Ala Leu Gln Ala Glu Ser Arg Gln Leu Val Asp Leu
405 410 415
Tyr Lys Val Leu Glu Ser Arg Gly Ser Asp Pro Lys Pro Glu Asn Pro
420 425 430
Ala Cys Pro Trp Thr Val Leu Pro Ala Gly Asp Leu Pro Thr His Asp
435 440 445
Gly Tyr Leu Pro Ser Asn Ile Asp Asp Leu Pro Ser His Glu Ala Pro
450 455 460
Leu Ala Asp Ser Leu Glu Glu Leu Glu Pro Gln His Ile Ser Leu Ser
465 470 475 480
Val Phe Pro Ser Ser Ser Leu His Pro Leu Thr Phe Ser Cys Gly Asp
485 490 495
Lys Leu Thr Leu Asp Gln Leu Lys Met Arg Cys Asp Ser Leu Met Leu
500 505 510
<210> 298
<211> 236
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 298
Met Leu Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala
1 5 10 15
Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser
20 25 30
Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn
35 40 45
Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro
50 55 60
Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro
65 70 75 80
Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His
85 90 95
Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly
100 105 110
Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg
115 120 125
Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp
130 135 140
Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr
145 150 155 160
Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Asn Leu Cys Tyr
165 170 175
Ile Leu Asp Ala Ile Leu Phe Leu Tyr Gly Ile Val Leu Thr Leu Leu
180 185 190
Tyr Cys Arg Leu Lys Ile Gln Val Arg Lys Ala Ala Ile Thr Ser Tyr
195 200 205
Glu Lys Ser Asp Gly Val Tyr Thr Gly Leu Ser Thr Arg Asn Gln Glu
210 215 220
Thr Tyr Glu Thr Leu Lys His Glu Lys Pro Pro Gln
225 230 235
<210> 299
<211> 273
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 299
Met Glu Gln Arg Gly Gln Asn Ala Pro Ala Ala Ser Gly Ala Arg Lys
1 5 10 15
Arg His Gly Pro Gly Pro Arg Glu Ala Arg Gly Ala Arg Pro Gly Pro
20 25 30
Arg Val Pro Lys Thr Leu Val Leu Val Val Ala Ala Val Leu Leu Leu
35 40 45
Val Ser Ala Glu Ser Ala Leu Ile Thr Gln Gln Asp Leu Ala Pro Gln
50 55 60
Gln Arg Ala Ala Pro Gln Gln Lys Arg Ser Ser Pro Ser Glu Gly Leu
65 70 75 80
Cys Pro Pro Gly His His Ile Ser Glu Asp Gly Arg Asp Cys Ile Ser
85 90 95
Cys Lys Tyr Gly Gln Asp Tyr Ser Thr His Trp Asn Asp Leu Leu Phe
100 105 110
Cys Leu Arg Cys Thr Arg Cys Asp Ser Gly Glu Val Glu Leu Ser Pro
115 120 125
Cys Thr Thr Thr Arg Asn Thr Val Cys Gln Cys Glu Glu Gly Thr Phe
130 135 140
Arg Glu Glu Asp Ser Pro Glu Met Cys Arg Lys Cys Arg Thr Gly Cys
145 150 155 160
Pro Arg Gly Met Val Lys Val Gly Asp Cys Thr Pro Trp Ser Asp Ile
165 170 175
Glu Cys Val His Lys Glu Ser Gly Thr Lys His Ser Gly Glu Val Pro
180 185 190
Ala Val Glu Glu Thr Val Thr Ser Ser Pro Gly Thr Pro Ala Ser Pro
195 200 205
Cys Ser Leu Ser Gly Ile Ile Ile Gly Val Thr Val Ala Ala Val Val
210 215 220
Leu Ile Val Ala Val Phe Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr
225 230 235 240
Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu
245 250 255
Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu
260 265 270
Leu
<210> 300
<211> 292
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 300
Met Gly Leu Ser Thr Val Pro Asp Leu Leu Leu Pro Leu Val Leu Leu
1 5 10 15
Glu Leu Leu Val Gly Ile Tyr Pro Ser Gly Val Ile Gly Leu Val Pro
20 25 30
His Leu Gly Asp Arg Glu Lys Arg Asp Ser Val Cys Pro Gln Gly Lys
35 40 45
Tyr Ile His Pro Gln Asn Asn Ser Ile Cys Cys Thr Lys Cys His Lys
50 55 60
Gly Thr Tyr Leu Tyr Asn Asp Cys Pro Gly Pro Gly Gln Asp Thr Asp
65 70 75 80
Cys Arg Glu Cys Glu Ser Gly Ser Phe Thr Ala Ser Glu Asn His Leu
85 90 95
Arg His Cys Leu Ser Cys Ser Lys Cys Arg Lys Glu Met Gly Gln Val
100 105 110
Glu Ile Ser Ser Cys Thr Val Asp Arg Asp Thr Val Cys Gly Cys Arg
115 120 125
Lys Asn Gln Tyr Arg His Tyr Trp Ser Glu Asn Leu Phe Gln Cys Phe
130 135 140
Asn Cys Ser Leu Cys Leu Asn Gly Thr Val His Leu Ser Cys Gln Glu
145 150 155 160
Lys Gln Asn Thr Val Cys Thr Cys His Ala Gly Phe Phe Leu Arg Glu
165 170 175
Asn Glu Cys Val Ser Cys Ser Asn Cys Lys Lys Ser Leu Glu Cys Thr
180 185 190
Lys Leu Cys Leu Pro Gln Ile Glu Asn Val Lys Gly Thr Glu Asp Ser
195 200 205
Gly Thr Thr Val Leu Leu Pro Leu Val Ile Phe Phe Gly Leu Cys Leu
210 215 220
Leu Ser Leu Leu Phe Ile Gly Leu Cys Val Lys Arg Arg Lys Pro Arg
225 230 235 240
Gly Asp Val Val Lys Val Ile Val Ser Val Gln Arg Lys Arg Gln Glu
245 250 255
Ala Glu Gly Glu Ala Thr Val Ile Glu Ala Leu Gln Ala Pro Pro Asp
260 265 270
Val Thr Thr Val Ala Val Glu Glu Thr Ile Pro Ser Phe Thr Gly Arg
275 280 285
Ser Pro Asn His
290
<210> 301
<211> 281
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 301
Met Gly Leu Ser Thr Val Pro Asp Leu Leu Leu Pro Leu Val Leu Leu
1 5 10 15
Glu Leu Leu Val Gly Ile Tyr Pro Ser Gly Val Ile Gly Leu Val Pro
20 25 30
His Leu Gly Asp Arg Glu Lys Arg Asp Ser Val Cys Pro Gln Gly Lys
35 40 45
Tyr Ile His Pro Gln Asn Asn Ser Ile Cys Cys Thr Lys Cys His Lys
50 55 60
Gly Thr Tyr Leu Tyr Asn Asp Cys Pro Gly Pro Gly Gln Asp Thr Asp
65 70 75 80
Cys Arg Glu Cys Glu Ser Gly Ser Phe Thr Ala Ser Glu Asn His Leu
85 90 95
Arg His Cys Leu Ser Cys Ser Lys Cys Arg Lys Glu Met Gly Gln Val
100 105 110
Glu Ile Ser Ser Cys Thr Val Asp Arg Asp Thr Val Cys Gly Cys Arg
115 120 125
Lys Asn Gln Tyr Arg His Tyr Trp Ser Glu Asn Leu Phe Gln Cys Phe
130 135 140
Asn Cys Ser Leu Cys Leu Asn Gly Thr Val His Leu Ser Cys Gln Glu
145 150 155 160
Lys Gln Asn Thr Val Cys Thr Cys His Ala Gly Phe Phe Leu Arg Glu
165 170 175
Asn Glu Cys Val Ser Cys Ser Asn Cys Lys Lys Ser Leu Glu Cys Thr
180 185 190
Lys Leu Cys Leu Pro Gln Ile Glu Asn Val Lys Gly Thr Glu Asp Ser
195 200 205
Gly Thr Thr Val Leu Leu Pro Leu Val Ile Phe Phe Gly Leu Cys Leu
210 215 220
Leu Ser Leu Leu Phe Ile Gly Leu His Gln Arg Arg Lys Tyr Arg Ser
225 230 235 240
Asn Lys Gly Glu Ser Pro Val Glu Pro Ala Glu Pro Cys His Tyr Ser
245 250 255
Cys Pro Arg Glu Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr
260 265 270
Arg Lys Pro Glu Pro Ala Cys Ser Pro
275 280
<210> 302
<211> 276
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 302
Met Gly Leu Ser Thr Val Pro Asp Leu Leu Leu Pro Leu Val Leu Leu
1 5 10 15
Glu Leu Leu Val Gly Ile Tyr Pro Ser Gly Val Ile Gly Leu Val Pro
20 25 30
His Leu Gly Asp Arg Glu Lys Arg Asp Ser Val Cys Pro Gln Gly Lys
35 40 45
Tyr Ile His Pro Gln Asn Asn Ser Ile Cys Cys Thr Lys Cys His Lys
50 55 60
Gly Thr Tyr Leu Tyr Asn Asp Cys Pro Gly Pro Gly Gln Asp Thr Asp
65 70 75 80
Cys Arg Glu Cys Glu Ser Gly Ser Phe Thr Ala Ser Glu Asn His Leu
85 90 95
Arg His Cys Leu Ser Cys Ser Lys Cys Arg Lys Glu Met Gly Gln Val
100 105 110
Glu Ile Ser Ser Cys Thr Val Asp Arg Asp Thr Val Cys Gly Cys Arg
115 120 125
Lys Asn Gln Tyr Arg His Tyr Trp Ser Glu Asn Leu Phe Gln Cys Phe
130 135 140
Asn Cys Ser Leu Cys Leu Asn Gly Thr Val His Leu Ser Cys Gln Glu
145 150 155 160
Lys Gln Asn Thr Val Cys Thr Cys His Ala Gly Phe Phe Leu Arg Glu
165 170 175
Asn Glu Cys Val Ser Cys Ser Asn Cys Lys Lys Ser Leu Glu Cys Thr
180 185 190
Lys Leu Cys Leu Pro Gln Ile Glu Asn Val Lys Gly Thr Glu Asp Ser
195 200 205
Gly Thr Thr Val Leu Leu Pro Leu Val Ile Phe Phe Gly Leu Cys Leu
210 215 220
Leu Ser Leu Leu Phe Ile Gly Leu Cys Val Lys Arg Gly Arg Lys Lys
225 230 235 240
Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr
245 250 255
Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly
260 265 270
Gly Cys Glu Leu
275
<210> 303
<211> 342
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 303
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe
275 280 285
Leu Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val Lys Arg Gly Arg
290 295 300
Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln
305 310 315 320
Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu
325 330 335
Glu Gly Gly Cys Glu Leu
340
<210> 304
<211> 491
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 304
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Met Phe Trp Val Gln Val Leu Leu Ala Gly Leu Val Val Pro Leu
275 280 285
Leu Leu Gly Ala Thr Leu Thr Tyr Thr Tyr Arg His Cys Trp Pro His
290 295 300
Lys Pro Leu Val Thr Ala Asp Glu Ala Gly Met Glu Ala Leu Thr Pro
305 310 315 320
Pro Pro Ala Thr His Leu Ser Pro Leu Asp Ser Ala His Thr Leu Leu
325 330 335
Ala Pro Pro Asp Ser Ser Glu Lys Ile Cys Thr Val Gln Leu Val Gly
340 345 350
Asn Ser Trp Thr Pro Gly Tyr Pro Glu Thr Gln Glu Ala Leu Cys Pro
355 360 365
Gln Val Thr Trp Ser Trp Asp Gln Leu Pro Ser Arg Ala Leu Gly Pro
370 375 380
Ala Ala Ala Pro Thr Leu Ser Pro Glu Ser Pro Ala Gly Ser Pro Ala
385 390 395 400
Met Met Leu Gln Pro Gly Pro Gln Leu Tyr Asp Val Met Asp Ala Val
405 410 415
Pro Ala Arg Arg Trp Lys Glu Phe Val Arg Thr Leu Gly Leu Arg Glu
420 425 430
Ala Glu Ile Glu Ala Val Glu Val Glu Ile Gly Arg Phe Arg Asp Gln
435 440 445
Gln Tyr Glu Met Leu Lys Arg Trp Arg Gln Gln Gln Pro Ala Gly Leu
450 455 460
Gly Ala Val Tyr Ala Ala Leu Glu Arg Met Gly Leu Asp Gly Cys Val
465 470 475 480
Glu Asp Leu Arg Ser Arg Leu Gln Arg Gly Pro
485 490
<210> 305
<211> 512
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 305
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Ile Phe Met Tyr Leu Leu Thr Val Phe Leu Ile Thr Gln Met Ile
275 280 285
Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg Arg Leu Asp Lys Ile
290 295 300
Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val Phe Met Lys Thr Ile
305 310 315 320
Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser Leu Leu Asn Cys Glu
325 330 335
Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys Asp Ile Met Leu Asn
340 345 350
Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu Met Gln Lys Gly Asp
355 360 365
Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys
370 375 380
Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser
385 390 395 400
Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg
405 410 415
Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg
420 425 430
Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser
435 440 445
Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser
450 455 460
Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe
465 470 475 480
Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser
485 490 495
Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu
500 505 510
<210> 306
<211> 332
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 306
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Phe Trp Leu Pro Ile Gly Cys Ala Ala Phe Val Val Val Cys Ile
275 280 285
Leu Gly Cys Ile Leu Ile Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser
290 295 300
Ser Val His Asp Pro Asn Gly Glu Tyr Met Phe Met Arg Ala Val Asn
305 310 315 320
Thr Ala Lys Lys Ser Arg Leu Thr Asp Val Thr Leu
325 330
<210> 307
<211> 336
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 307
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Val Ala Ala Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly
275 280 285
Pro Leu Ala Ile Leu Leu Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg
290 295 300
Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr
305 310 315 320
Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile
325 330 335
<210> 308
<211> 476
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 308
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Val Gly Leu Gly Leu Leu Leu Leu Leu Met Gly Ala Gly Leu Ala
275 280 285
Val Gln Gly Trp Phe Leu Leu Gln Leu His Trp Arg Leu Gly Glu Met
290 295 300
Val Thr Arg Leu Pro Asp Gly Pro Ala Gly Ser Trp Glu Gln Leu Ile
305 310 315 320
Gln Glu Arg Arg Ser His Glu Val Asn Pro Ala Ala His Leu Thr Gly
325 330 335
Ala Asn Ser Ser Leu Thr Gly Ser Gly Gly Pro Leu Leu Trp Glu Thr
340 345 350
Gln Leu Gly Leu Ala Phe Leu Arg Gly Leu Ser Tyr His Asp Gly Ala
355 360 365
Leu Val Val Thr Lys Ala Gly Tyr Tyr Tyr Ile Tyr Ser Lys Val Gln
370 375 380
Leu Gly Gly Val Gly Cys Pro Leu Gly Leu Ala Ser Thr Ile Thr His
385 390 395 400
Gly Leu Tyr Lys Arg Thr Pro Arg Tyr Pro Glu Glu Leu Glu Leu Leu
405 410 415
Val Ser Gln Gln Ser Pro Cys Gly Arg Ala Thr Ser Ser Ser Arg Val
420 425 430
Trp Trp Asp Ser Ser Phe Leu Gly Gly Val Val His Leu Glu Ala Gly
435 440 445
Glu Lys Val Val Val Arg Val Leu Asp Glu Arg Leu Val Arg Leu Arg
450 455 460
Asp Gly Thr Arg Ser Tyr Phe Gly Ala Phe Met Val
465 470 475
<210> 309
<211> 489
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 309
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Pro Val Leu Asp Ala Gly Pro Val Leu Phe Trp Val Ile Leu Val
275 280 285
Leu Val Val Val Val Gly Ser Ser Ala Phe Leu Leu Cys His Arg Arg
290 295 300
Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys Tyr Pro Val
305 310 315 320
Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg Pro Arg Arg
325 330 335
Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu Pro Val Ala
340 345 350
Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr Cys His Ser
355 360 365
Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp Ala Ser Pro
370 375 380
Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro Arg Val Ser
385 390 395 400
Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile Met Lys Ala
405 410 415
Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro Glu Gly Arg
420 425 430
Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu Leu Glu Ala
435 440 445
Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro Pro Leu Gly
450 455 460
Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly Lys Glu Asp
465 470 475 480
Pro Leu Pro Thr Ala Ala Ser Gly Lys
485
<210> 310
<211> 352
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 310
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Leu Gly Trp Leu Thr Val Val Leu Leu Ala Val Ala Ala Cys Val
275 280 285
Leu Leu Leu Thr Ser Ala Gln Leu Gly Leu His Ile Trp Gln Leu Arg
290 295 300
Ser Gln Cys Met Trp Pro Arg Glu Thr Gln Leu Leu Leu Glu Val Pro
305 310 315 320
Pro Ser Thr Glu Asp Ala Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg
325 330 335
Gly Glu Arg Ser Ala Glu Glu Lys Gly Arg Leu Gly Asp Leu Trp Val
340 345 350
<210> 311
<211> 438
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 311
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Pro Phe Phe Phe Cys Cys Phe Ile Ala Val Ala Met Gly Ile Arg
275 280 285
Phe Ile Ile Met Val Ala Ile Trp Ser Ala Val Phe Leu Asn Ser Leu
290 295 300
Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro
305 310 315 320
Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe
325 330 335
Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln
340 345 350
Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu
355 360 365
Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile Pro Thr
370 375 380
Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu
385 390 395 400
Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser
405 410 415
Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile
420 425 430
Cys Met Gln Arg Thr Val
435
<210> 312
<211> 355
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 312
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Gly Gly Thr Val Leu Leu Leu Leu Phe Val Ile Ser Ile Thr Thr
275 280 285
Ile Ile Val Ile Phe Leu Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp
290 295 300
Leu Phe Thr Glu Ser Trp Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg
305 310 315 320
Ser Pro Ile Ser Thr Ser Gln Pro Thr Asn Gln Ser Met Asp Asp Thr
325 330 335
Arg Glu Asp Ile Tyr Val Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys
340 345 350
Thr Arg Val
355
<210> 313
<211> 371
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 313
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Trp Ala Val Tyr Ala Gly Leu Leu Gly Gly Val Ile Met Ile Leu
275 280 285
Ile Met Val Val Ile Leu Gln Leu Arg Arg Arg Gly Lys Thr Asn His
290 295 300
Tyr Gln Thr Thr Val Glu Lys Lys Ser Leu Thr Ile Tyr Ala Gln Val
305 310 315 320
Gln Lys Pro Gly Pro Leu Gln Lys Lys Leu Asp Ser Phe Pro Ala Gln
325 330 335
Asp Pro Cys Thr Thr Ile Tyr Val Ala Ala Thr Glu Pro Val Pro Glu
340 345 350
Ser Val Gln Glu Thr Asn Ser Ile Thr Val Tyr Ala Ser Val Thr Leu
355 360 365
Pro Glu Ser
370
<210> 314
<211> 342
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 314
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Ile Tyr Ala Gly Val Cys Ile Ser Val Leu Val Leu Leu Ala Leu
275 280 285
Leu Gly Val Ile Ile Ala Lys Lys Tyr Phe Phe Lys Lys Glu Val Gln
290 295 300
Gln Leu Ser Val Ser Phe Ser Ser Leu Gln Ile Lys Ala Leu Gln Asn
305 310 315 320
Ala Val Glu Lys Glu Val Gln Ala Glu Asp Asn Ile Tyr Ile Glu Asn
325 330 335
Ser Leu Tyr Ala Thr Asp
340
<210> 315
<211> 342
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 315
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Ile Leu Val Ile Phe Ser Gly Met Phe Leu Val Phe Thr Leu Ala
275 280 285
Gly Ala Leu Phe Leu His Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly
290 295 300
Glu Ser Pro Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Arg
305 310 315 320
Glu Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro
325 330 335
Glu Pro Ala Cys Ser Pro
340
<210> 316
<211> 354
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 316
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Trp Trp Phe Leu Ser Gly Ser Leu Val Ile Val Ile Val Cys Ser
275 280 285
Thr Val Gly Leu Ile Ile Cys Val Lys Arg Arg Lys Pro Arg Gly Asp
290 295 300
Val Val Lys Val Ile Val Ser Val Gln Arg Lys Arg Gln Glu Ala Glu
305 310 315 320
Gly Glu Ala Thr Val Ile Glu Ala Leu Gln Ala Pro Pro Asp Val Thr
325 330 335
Thr Val Ala Val Glu Glu Thr Ile Pro Ser Phe Thr Gly Arg Ser Pro
340 345 350
Asn His
<210> 317
<211> 405
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 317
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Phe Leu Phe Val Leu Leu Gly Val Gly Ser Met Gly Val Ala Ala
275 280 285
Ile Val Trp Gly Ala Trp Phe Trp Gly Arg Arg Ser Cys Gln Gln Arg
290 295 300
Asp Ser Gly Asn Ser Pro Gly Asn Ala Phe Tyr Ser Asn Val Leu Tyr
305 310 315 320
Arg Pro Arg Gly Ala Pro Lys Lys Ser Glu Asp Cys Ser Gly Glu Gly
325 330 335
Lys Asp Gln Arg Gly Gln Ser Ile Tyr Ser Thr Ser Phe Pro Gln Pro
340 345 350
Ala Pro Arg Gln Pro His Leu Ala Ser Arg Pro Cys Pro Ser Pro Arg
355 360 365
Pro Cys Pro Ser Pro Arg Pro Gly His Pro Val Ser Met Val Arg Val
370 375 380
Ser Pro Arg Pro Ser Pro Thr Gln Gln Pro Arg Pro Lys Gly Phe Pro
385 390 395 400
Lys Val Gly Glu Glu
405
<210> 318
<211> 341
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 318
Met Ala Val Met Ala Pro Arg Thr Leu Val Leu Leu Leu Ser Gly Ala
1 5 10 15
Leu Ala Leu Thr Gln Thr Trp Ala Gln Val Gln Leu Val Gln Ser Gly
20 25 30
Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala
35 40 45
Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Ile His Trp Val Arg Gln Ala
50 55 60
Pro Gly Gln Gly Leu Glu Trp Ile Gly Cys Ile Tyr Pro Gly Asn Val
65 70 75 80
Asn Thr Asn Tyr Asn Glu Lys Phe Lys Asp Arg Ala Thr Leu Thr Val
85 90 95
Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser
100 105 110
Asp Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser His Tyr Gly Leu Asp
115 120 125
Trp Asn Phe Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser
130 135 140
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
145 150 155 160
Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val
165 170 175
Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Tyr Val
180 185 190
Trp Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu
195 200 205
Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser
210 215 220
Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln
225 230 235 240
Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Thr Tyr Pro
245 250 255
Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly
260 265 270
Ser Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser
275 280 285
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg
290 295 300
Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Pro Pro Arg Arg Pro
305 310 315 320
Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe
325 330 335
Ala Ala Tyr Arg Ser
340
<210> 319
<211> 278
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 319
Met Gln Ser Gly Thr His Trp Arg Val Leu Gly Leu Cys Leu Leu Ser
1 5 10 15
Val Gly Val Trp Gly Gln Gly Val Thr Leu Phe Val Ala Leu Tyr Asp
20 25 30
Tyr Glu Ala Arg Gly Leu Asn Arg Met Phe Asp Leu Ser Phe His Lys
35 40 45
Gly Glu Lys Phe Gln Ile Leu Ser Phe Glu Thr Gly Asp Trp Trp Glu
50 55 60
Ala Arg Ser Leu Thr Thr Gly Glu Thr Gly Tyr Ile Pro Ser Asn Tyr
65 70 75 80
Val Ala Pro Val Asp Ser Ile Gln Gly Gly Gly Gly Ser Asp Gly Asn
85 90 95
Glu Glu Met Gly Gly Ile Thr Gln Thr Pro Tyr Lys Val Ser Ile Ser
100 105 110
Gly Thr Thr Val Ile Leu Thr Cys Pro Gln Tyr Pro Gly Ser Glu Ile
115 120 125
Leu Trp Gln His Asn Asp Lys Asn Ile Gly Gly Asp Glu Asp Asp Lys
130 135 140
Asn Ile Gly Ser Asp Glu Asp His Leu Ser Leu Lys Glu Phe Ser Glu
145 150 155 160
Leu Glu Gln Ser Gly Tyr Tyr Val Cys Tyr Pro Arg Gly Ser Lys Pro
165 170 175
Glu Asp Ala Asn Phe Tyr Leu Tyr Leu Arg Ala Arg Val Cys Glu Asn
180 185 190
Cys Met Glu Met Asp Val Met Ser Val Ala Thr Ile Val Ile Val Asp
195 200 205
Ile Cys Ile Thr Gly Gly Leu Leu Leu Leu Val Tyr Tyr Trp Ser Lys
210 215 220
Asn Arg Lys Ala Lys Ala Lys Pro Val Thr Arg Gly Ala Gly Ala Gly
225 230 235 240
Gly Arg Gln Arg Gly Gln Asn Lys Glu Arg Pro Pro Pro Val Pro Asn
245 250 255
Pro Asp Tyr Glu Pro Ile Arg Lys Gly Gln Arg Asp Leu Tyr Ser Gly
260 265 270
Leu Asn Gln Arg Arg Ile
275
<210> 320
<211> 243
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 320
Met Gln Ser Gly Thr His Trp Arg Val Leu Gly Leu Cys Leu Leu Ser
1 5 10 15
Val Gly Val Trp Gly Gln Gly Val Thr Leu Phe Val Ala Leu Tyr Asp
20 25 30
Tyr Glu Ala Arg Gly Leu Asn Arg Met Phe Asp Leu Ser Phe His Lys
35 40 45
Gly Glu Lys Phe Gln Ile Leu Ser Phe Glu Thr Gly Asp Trp Trp Glu
50 55 60
Ala Arg Ser Leu Thr Thr Gly Glu Thr Gly Tyr Ile Pro Ser Asn Tyr
65 70 75 80
Val Ala Pro Val Asp Ser Ile Gln Gly Gly Gly Gly Ser Phe Lys Ile
85 90 95
Pro Ile Glu Glu Leu Glu Asp Arg Val Phe Val Asn Cys Asn Thr Ser
100 105 110
Ile Thr Trp Val Glu Gly Thr Val Gly Thr Leu Leu Ser Asp Ile Thr
115 120 125
Arg Leu Asp Leu Gly Lys Arg Ile Leu Asp Pro Arg Gly Ile Tyr Arg
130 135 140
Cys Asn Gly Thr Asp Ile Tyr Lys Asp Lys Glu Ser Thr Val Gln Val
145 150 155 160
His Tyr Arg Met Cys Gln Ser Cys Val Glu Leu Asp Pro Ala Thr Val
165 170 175
Ala Gly Ile Ile Val Thr Asp Val Ile Ala Thr Leu Leu Leu Ala Leu
180 185 190
Gly Val Phe Cys Phe Ala Gly His Glu Thr Gly Arg Leu Ser Gly Ala
195 200 205
Ala Asp Thr Gln Ala Leu Leu Arg Asn Asp Gln Val Tyr Gln Pro Leu
210 215 220
Arg Asp Arg Asp Asp Ala Gln Tyr Ser His Leu Gly Gly Asn Trp Ala
225 230 235 240
Arg Asn Lys
<210> 321
<211> 253
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 321
Met Gln Ser Gly Thr His Trp Arg Val Leu Gly Leu Cys Leu Leu Ser
1 5 10 15
Val Gly Val Trp Gly Gln Gly Val Thr Leu Phe Val Ala Leu Tyr Asp
20 25 30
Tyr Glu Ala Arg Gly Leu Asn Arg Met Phe Asp Leu Ser Phe His Lys
35 40 45
Gly Glu Lys Phe Gln Ile Leu Ser Phe Glu Thr Gly Asp Trp Trp Glu
50 55 60
Ala Arg Ser Leu Thr Thr Gly Glu Thr Gly Tyr Ile Pro Ser Asn Tyr
65 70 75 80
Val Ala Pro Val Asp Ser Ile Gln Gly Gly Gly Gly Ser Gln Ser Ile
85 90 95
Lys Gly Asn His Leu Val Lys Val Tyr Asp Tyr Gln Glu Asp Gly Ser
100 105 110
Val Leu Leu Thr Cys Asp Ala Glu Ala Lys Asn Ile Thr Trp Phe Lys
115 120 125
Asp Gly Lys Met Ile Gly Phe Leu Thr Glu Asp Lys Lys Lys Trp Asn
130 135 140
Leu Gly Ser Asn Ala Lys Asp Pro Arg Gly Met Tyr Gln Cys Lys Gly
145 150 155 160
Ser Gln Asn Lys Ser Lys Pro Leu Gln Val Tyr Tyr Arg Met Cys Gln
165 170 175
Asn Cys Ile Glu Leu Asn Ala Ala Thr Ile Ser Gly Phe Leu Phe Ala
180 185 190
Glu Ile Val Ser Ile Phe Val Leu Ala Val Gly Val Tyr Phe Ile Ala
195 200 205
Gly Gln Asp Gly Val Arg Gln Ser Arg Ala Ser Asp Lys Gln Thr Leu
210 215 220
Leu Pro Asn Asp Gln Leu Tyr Gln Pro Leu Lys Asp Arg Glu Asp Asp
225 230 235 240
Gln Tyr Ser His Leu Gln Gly Asn Gln Leu Arg Arg Asn
245 250
<210> 322
<211> 678
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 322
Gln Glu Cys Thr Lys Phe Lys Val Ser Ser Cys Arg Glu Cys Ile Glu
1 5 10 15
Ser Gly Pro Gly Cys Thr Trp Cys Gln Lys Leu Asn Phe Thr Gly Pro
20 25 30
Gly Asp Pro Asp Ser Ile Arg Cys Asp Thr Arg Pro Gln Leu Leu Met
35 40 45
Arg Gly Cys Ala Ala Asp Asp Ile Met Asp Pro Thr Ser Leu Ala Glu
50 55 60
Thr Gln Glu Asp His Asn Gly Gly Gln Lys Gln Leu Ser Pro Gln Lys
65 70 75 80
Val Thr Leu Tyr Leu Arg Pro Gly Gln Ala Ala Ala Phe Asn Val Thr
85 90 95
Phe Arg Arg Ala Lys Gly Tyr Pro Ile Asp Leu Tyr Tyr Leu Met Asp
100 105 110
Leu Ser Tyr Ser Met Leu Asp Asp Leu Arg Asn Val Lys Lys Leu Gly
115 120 125
Gly Asp Leu Leu Arg Ala Leu Asn Glu Ile Thr Glu Ser Gly Arg Ile
130 135 140
Gly Phe Gly Ser Phe Val Asp Lys Thr Val Leu Pro Phe Val Asn Thr
145 150 155 160
His Pro Asp Lys Leu Arg Asn Pro Cys Pro Asn Lys Glu Lys Glu Cys
165 170 175
Gln Pro Pro Phe Ala Phe Arg His Val Leu Lys Leu Thr Asn Asn Ser
180 185 190
Asn Gln Phe Gln Thr Glu Val Gly Lys Gln Leu Ile Ser Gly Asn Leu
195 200 205
Asp Ala Pro Glu Gly Gly Leu Asp Ala Met Met Gln Val Ala Ala Cys
210 215 220
Pro Glu Glu Ile Gly Trp Arg Asn Val Thr Arg Leu Leu Val Phe Ala
225 230 235 240
Thr Asp Asp Gly Phe His Phe Ala Gly Asp Gly Lys Leu Gly Ala Ile
245 250 255
Leu Thr Pro Asn Asp Gly Arg Cys His Leu Glu Asp Asn Leu Tyr Lys
260 265 270
Arg Ser Asn Glu Phe Asp Tyr Pro Ser Val Gly Gln Leu Ala His Lys
275 280 285
Leu Ala Glu Asn Asn Ile Gln Pro Ile Phe Ala Val Thr Ser Arg Met
290 295 300
Val Lys Thr Tyr Glu Lys Leu Thr Glu Ile Ile Pro Lys Ser Ala Val
305 310 315 320
Gly Glu Leu Ser Glu Asp Ser Ser Asn Val Val Gln Leu Ile Lys Asn
325 330 335
Ala Tyr Asn Lys Leu Ser Ser Arg Val Phe Leu Asp His Asn Ala Leu
340 345 350
Pro Asp Thr Leu Lys Val Thr Tyr Asp Ser Phe Cys Ser Asn Gly Val
355 360 365
Thr His Arg Asn Gln Pro Arg Gly Asp Cys Asp Gly Val Gln Ile Asn
370 375 380
Val Pro Ile Thr Phe Gln Val Lys Val Thr Ala Thr Glu Cys Ile Gln
385 390 395 400
Glu Gln Ser Phe Val Ile Arg Ala Leu Gly Phe Thr Asp Ile Val Thr
405 410 415
Val Gln Val Leu Pro Gln Cys Glu Cys Arg Cys Arg Asp Gln Ser Arg
420 425 430
Asp Arg Ser Leu Cys His Gly Lys Gly Phe Leu Glu Cys Gly Ile Cys
435 440 445
Arg Cys Asp Thr Gly Tyr Ile Gly Lys Asn Cys Glu Cys Gln Thr Gln
450 455 460
Gly Arg Ser Ser Gln Glu Leu Glu Gly Ser Cys Arg Lys Asp Asn Asn
465 470 475 480
Ser Ile Ile Cys Ser Gly Leu Gly Asp Cys Val Cys Gly Gln Cys Leu
485 490 495
Cys His Thr Ser Asp Val Pro Gly Lys Leu Ile Tyr Gly Gln Tyr Cys
500 505 510
Glu Cys Asp Thr Ile Asn Cys Glu Arg Tyr Asn Gly Gln Val Cys Gly
515 520 525
Gly Pro Gly Arg Gly Leu Cys Phe Cys Gly Lys Cys Arg Cys His Pro
530 535 540
Gly Phe Glu Gly Ser Ala Cys Gln Cys Glu Arg Thr Thr Glu Gly Cys
545 550 555 560
Leu Asn Pro Arg Arg Val Glu Cys Ser Gly Arg Gly Arg Cys Arg Cys
565 570 575
Asn Val Cys Glu Cys His Ser Gly Tyr Gln Leu Pro Leu Cys Gln Glu
580 585 590
Cys Pro Gly Cys Pro Ser Pro Cys Gly Lys Tyr Ile Ser Cys Ala Glu
595 600 605
Cys Leu Lys Phe Glu Lys Gly Pro Phe Gly Lys Asn Cys Ser Ala Ala
610 615 620
Cys Pro Gly Leu Gln Leu Ser Asn Asn Pro Val Lys Gly Arg Thr Cys
625 630 635 640
Lys Glu Arg Asp Ser Glu Gly Cys Trp Val Ala Tyr Thr Leu Glu Gln
645 650 655
Gln Asp Gly Met Asp Arg Tyr Leu Ile Tyr Val Asp Glu Ser Arg Glu
660 665 670
Cys Val Ala Gly Pro Asn
675
<210> 323
<211> 148
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 323
Gln Val Thr Asp Ile Asn Ser Lys Gly Leu Glu Leu Arg Lys Thr Val
1 5 10 15
Thr Thr Val Glu Thr Gln Asn Leu Glu Gly Leu His His Asp Gly Gln
20 25 30
Phe Cys His Lys Pro Cys Pro Pro Gly Glu Arg Lys Ala Arg Asp Cys
35 40 45
Thr Val Asn Gly Asp Glu Pro Asp Cys Val Pro Cys Gln Glu Gly Lys
50 55 60
Glu Tyr Thr Asp Lys Ala His Phe Ser Ser Lys Cys Arg Arg Cys Arg
65 70 75 80
Leu Cys Asp Glu Gly His Gly Leu Glu Val Glu Ile Asn Cys Thr Arg
85 90 95
Thr Gln Asn Thr Lys Cys Arg Cys Lys Pro Asn Phe Phe Cys Asn Ser
100 105 110
Thr Val Cys Glu His Cys Asp Pro Cys Thr Lys Cys Glu His Gly Ile
115 120 125
Ile Lys Glu Cys Thr Leu Thr Ser Asn Thr Lys Cys Lys Glu Glu Gly
130 135 140
Ser Arg Ser Asn
145
<210> 324
<211> 361
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 324
Phe Pro Gln Asp Arg Pro Phe Glu Asp Thr Cys His Gly Asn Pro Ser
1 5 10 15
His Tyr Tyr Asp Lys Ala Val Arg Arg Cys Cys Tyr Arg Cys Pro Met
20 25 30
Gly Leu Phe Pro Thr Gln Gln Cys Pro Gln Arg Pro Thr Asp Cys Arg
35 40 45
Lys Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Asp Arg Cys Thr
50 55 60
Ala Cys Val Thr Cys Ser Arg Asp Asp Leu Val Glu Lys Thr Pro Cys
65 70 75 80
Ala Trp Asn Ser Ser Arg Val Cys Glu Cys Arg Pro Gly Met Phe Cys
85 90 95
Ser Thr Ser Ala Val Asn Ser Cys Ala Arg Cys Phe Phe His Ser Val
100 105 110
Cys Pro Ala Gly Met Ile Val Lys Phe Pro Gly Thr Ala Gln Lys Asn
115 120 125
Thr Val Cys Glu Pro Ala Ser Pro Gly Val Ser Pro Ala Cys Ala Ser
130 135 140
Pro Glu Asn Cys Lys Glu Pro Ser Ser Gly Thr Ile Pro Gln Ala Lys
145 150 155 160
Pro Thr Pro Val Ser Pro Ala Thr Ser Ser Ala Ser Thr Met Pro Val
165 170 175
Arg Gly Gly Thr Arg Leu Ala Gln Glu Ala Ala Ser Lys Leu Thr Arg
180 185 190
Ala Pro Asp Ser Pro Ser Ser Val Gly Arg Pro Ser Ser Asp Pro Gly
195 200 205
Leu Ser Pro Thr Gln Pro Cys Pro Glu Gly Ser Gly Asp Cys Arg Lys
210 215 220
Gln Cys Glu Pro Asp Tyr Tyr Leu Asp Glu Ala Gly Arg Cys Thr Ala
225 230 235 240
Cys Val Ser Cys Ser Arg Asp Asp Leu Val Glu Lys Thr Pro Cys Ala
245 250 255
Trp Asn Ser Ser Arg Thr Cys Glu Cys Arg Pro Gly Met Ile Cys Ala
260 265 270
Thr Ser Ala Thr Asn Ser Cys Ala Arg Cys Val Pro Tyr Pro Ile Cys
275 280 285
Ala Ala Glu Thr Val Thr Lys Pro Gln Asp Met Ala Glu Lys Asp Thr
290 295 300
Thr Phe Glu Ala Pro Pro Leu Gly Thr Gln Pro Asp Cys Asn Pro Thr
305 310 315 320
Pro Glu Asn Gly Glu Ala Pro Ala Ser Thr Ser Pro Thr Gln Ser Leu
325 330 335
Leu Val Asp Ser Gln Ala Ser Lys Thr Leu Pro Ile Pro Thr Ser Ala
340 345 350
Pro Val Ala Leu Ser Ser Thr Gly Lys
355 360
<210> 325
<211> 216
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 325
Ala Ser Gly Thr Glu Ala Ala Ala Ala Thr Pro Ser Lys Val Trp Gly
1 5 10 15
Ser Ser Ala Gly Arg Ile Glu Pro Arg Gly Gly Gly Arg Gly Ala Leu
20 25 30
Pro Thr Ser Met Gly Gln His Gly Pro Ser Ala Arg Ala Arg Ala Gly
35 40 45
Arg Ala Pro Gly Pro Arg Pro Ala Arg Glu Ala Ser Pro Arg Leu Arg
50 55 60
Val His Lys Thr Phe Lys Phe Val Val Val Gly Val Leu Leu Gln Val
65 70 75 80
Val Pro Ser Ser Ala Ala Thr Ile Lys Leu His Asp Gln Ser Ile Gly
85 90 95
Thr Gln Gln Trp Glu His Ser Pro Leu Gly Glu Leu Cys Pro Pro Gly
100 105 110
Ser His Arg Ser Glu His Pro Gly Ala Cys Asn Arg Cys Thr Glu Gly
115 120 125
Val Gly Tyr Thr Asn Ala Ser Asn Asn Leu Phe Ala Cys Leu Pro Cys
130 135 140
Thr Ala Cys Lys Ser Asp Glu Glu Glu Arg Ser Pro Cys Thr Thr Thr
145 150 155 160
Arg Asn Thr Ala Cys Gln Cys Lys Pro Gly Thr Phe Arg Asn Asp Asn
165 170 175
Ser Ala Glu Met Cys Arg Lys Cys Ser Arg Gly Cys Pro Arg Gly Met
180 185 190
Val Lys Val Lys Asp Cys Thr Pro Trp Ser Asp Ile Glu Cys Val His
195 200 205
Lys Glu Ser Gly Asn Gly His Asn
210 215
<210> 326
<211> 155
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 326
Ile Thr Gln Gln Asp Leu Ala Pro Gln Gln Arg Ala Ala Pro Gln Gln
1 5 10 15
Lys Arg Ser Ser Pro Ser Glu Gly Leu Cys Pro Pro Gly His His Ile
20 25 30
Ser Glu Asp Gly Arg Asp Cys Ile Ser Cys Lys Tyr Gly Gln Asp Tyr
35 40 45
Ser Thr His Trp Asn Asp Leu Leu Phe Cys Leu Arg Cys Thr Arg Cys
50 55 60
Asp Ser Gly Glu Val Glu Leu Ser Pro Cys Thr Thr Thr Arg Asn Thr
65 70 75 80
Val Cys Gln Cys Glu Glu Gly Thr Phe Arg Glu Glu Asp Ser Pro Glu
85 90 95
Met Cys Arg Lys Cys Arg Thr Gly Cys Pro Arg Gly Met Val Lys Val
100 105 110
Gly Asp Cys Thr Pro Trp Ser Asp Ile Glu Cys Val His Lys Glu Ser
115 120 125
Gly Thr Lys His Ser Gly Glu Val Pro Ala Val Glu Glu Thr Val Thr
130 135 140
Ser Ser Pro Gly Thr Pro Ala Ser Pro Cys Ser
145 150 155
<210> 327
<211> 141
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 327
Glu Ser Gly Asp Cys Arg Gln Gln Glu Phe Arg Asp Arg Ser Gly Asn
1 5 10 15
Cys Val Pro Cys Asn Gln Cys Gly Pro Gly Met Glu Leu Ser Lys Glu
20 25 30
Cys Gly Phe Gly Tyr Gly Glu Asp Ala Gln Cys Val Thr Cys Arg Leu
35 40 45
His Arg Phe Lys Glu Asp Trp Gly Phe Gln Lys Cys Lys Pro Cys Leu
50 55 60
Asp Cys Ala Val Val Asn Arg Phe Gln Lys Ala Asn Cys Ser Ala Thr
65 70 75 80
Ser Asp Ala Ile Cys Gly Asp Cys Leu Pro Gly Phe Tyr Arg Lys Thr
85 90 95
Lys Leu Val Gly Phe Gln Asp Met Glu Cys Val Pro Cys Gly Asp Pro
100 105 110
Pro Pro Pro Tyr Glu Pro His Cys Ala Ser Lys Val Asn Leu Val Lys
115 120 125
Ile Ala Ser Thr Ala Ser Ser Pro Arg Asp Thr Ala Leu
130 135 140
<210> 328
<211> 308
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 328
Gln Pro Glu Gln Lys Ala Ser Asn Leu Ile Gly Thr Tyr Arg His Val
1 5 10 15
Asp Arg Ala Thr Gly Gln Val Leu Thr Cys Asp Lys Cys Pro Ala Gly
20 25 30
Thr Tyr Val Ser Glu His Cys Thr Asn Thr Ser Leu Arg Val Cys Ser
35 40 45
Ser Cys Pro Val Gly Thr Phe Thr Arg His Glu Asn Gly Ile Glu Lys
50 55 60
Cys His Asp Cys Ser Gln Pro Cys Pro Trp Pro Met Ile Glu Lys Leu
65 70 75 80
Pro Cys Ala Ala Leu Thr Asp Arg Glu Cys Thr Cys Pro Pro Gly Met
85 90 95
Phe Gln Ser Asn Ala Thr Cys Ala Pro His Thr Val Cys Pro Val Gly
100 105 110
Trp Gly Val Arg Lys Lys Gly Thr Glu Thr Glu Asp Val Arg Cys Lys
115 120 125
Gln Cys Ala Arg Gly Thr Phe Ser Asp Val Pro Ser Ser Val Met Lys
130 135 140
Cys Lys Ala Tyr Thr Asp Cys Leu Ser Gln Asn Leu Val Val Ile Lys
145 150 155 160
Pro Gly Thr Lys Glu Thr Asp Asn Val Cys Gly Thr Leu Pro Ser Phe
165 170 175
Ser Ser Ser Thr Ser Pro Ser Pro Gly Thr Ala Ile Phe Pro Arg Pro
180 185 190
Glu His Met Glu Thr His Glu Val Pro Ser Ser Thr Tyr Val Pro Lys
195 200 205
Gly Met Asn Ser Thr Glu Ser Asn Ser Ser Ala Ser Val Arg Pro Lys
210 215 220
Val Leu Ser Ser Ile Gln Glu Gly Thr Val Pro Asp Asn Thr Ser Ser
225 230 235 240
Ala Arg Gly Lys Glu Asp Val Asn Lys Thr Leu Pro Asn Leu Gln Val
245 250 255
Val Asn His Gln Gln Gly Pro His His Arg His Ile Leu Lys Leu Leu
260 265 270
Pro Ser Met Glu Ala Thr Gly Gly Glu Lys Ser Ser Thr Pro Ile Lys
275 280 285
Gly Pro Lys Arg Gly His Pro Arg Gln Asn Leu His Lys His Phe Asp
290 295 300
Ile Asn Glu His
305
<210> 329
<211> 126
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 329
Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg
1 5 10 15
Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr
20 25 30
Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu
35 40 45
Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp
50 55 60
Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr
65 70 75 80
Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val
85 90 95
Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr
100 105 110
Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp
115 120 125
<210> 330
<211> 182
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 330
Leu Val Pro His Leu Gly Asp Arg Glu Lys Arg Asp Ser Val Cys Pro
1 5 10 15
Gln Gly Lys Tyr Ile His Pro Gln Asn Asn Ser Ile Cys Cys Thr Lys
20 25 30
Cys His Lys Gly Thr Tyr Leu Tyr Asn Asp Cys Pro Gly Pro Gly Gln
35 40 45
Asp Thr Asp Cys Arg Glu Cys Glu Ser Gly Ser Phe Thr Ala Ser Glu
50 55 60
Asn His Leu Arg His Cys Leu Ser Cys Ser Lys Cys Arg Lys Glu Met
65 70 75 80
Gly Gln Val Glu Ile Ser Ser Cys Thr Val Asp Arg Asp Thr Val Cys
85 90 95
Gly Cys Arg Lys Asn Gln Tyr Arg His Tyr Trp Ser Glu Asn Leu Phe
100 105 110
Gln Cys Phe Asn Cys Ser Leu Cys Leu Asn Gly Thr Val His Leu Ser
115 120 125
Cys Gln Glu Lys Gln Asn Thr Val Cys Thr Cys His Ala Gly Phe Phe
130 135 140
Leu Arg Glu Asn Glu Cys Val Ser Cys Ser Asn Cys Lys Lys Ser Leu
145 150 155 160
Glu Cys Thr Lys Leu Cys Leu Pro Gln Ile Glu Asn Val Lys Gly Thr
165 170 175
Glu Asp Ser Gly Thr Thr
180
<210> 331
<211> 133
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 331
Gln Val Ser His Arg Tyr Pro Arg Ile Gln Ser Ile Lys Val Gln Phe
1 5 10 15
Thr Glu Tyr Lys Lys Glu Lys Gly Phe Ile Leu Thr Ser Gln Lys Glu
20 25 30
Asp Glu Ile Met Lys Val Gln Asn Asn Ser Val Ile Ile Asn Cys Asp
35 40 45
Gly Phe Tyr Leu Ile Ser Leu Lys Gly Tyr Phe Ser Gln Glu Val Asn
50 55 60
Ile Ser Leu His Tyr Gln Lys Asp Glu Glu Pro Leu Phe Gln Leu Lys
65 70 75 80
Lys Val Arg Ser Val Asn Ser Leu Met Val Ala Ser Leu Thr Tyr Lys
85 90 95
Asp Lys Val Tyr Leu Asn Val Thr Thr Asp Asn Thr Ser Leu Asp Asp
100 105 110
Phe His Val Asn Gly Gly Glu Leu Ile Leu Ile His Gln Asn Pro Gly
115 120 125
Glu Phe Cys Val Leu
130
<210> 332
<211> 215
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 332
His Arg Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp
1 5 10 15
Phe Val Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser
20 25 30
Leu Ser Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe
35 40 45
Val Lys Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser
50 55 60
Phe Glu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val
65 70 75 80
Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu
85 90 95
Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly
100 105 110
Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln
115 120 125
Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile
130 135 140
Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu
145 150 155 160
Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser
165 170 175
Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe
180 185 190
Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr
195 200 205
Ser Phe Gly Leu Leu Lys Leu
210 215
<210> 333
<211> 205
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 333
Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser Ala
1 5 10 15
Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp Pro
20 25 30
Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala
35 40 45
Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro
50 55 60
Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp
65 70 75 80
Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe
85 90 95
Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val
100 105 110
Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala
115 120 125
Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg
130 135 140
Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly
145 150 155 160
Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala
165 170 175
Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Thr
180 185 190
Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu
195 200 205
<210> 334
<211> 238
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 334
Asp Thr Gln Glu Lys Glu Val Arg Ala Met Val Gly Ser Asp Val Glu
1 5 10 15
Leu Ser Cys Ala Cys Pro Glu Gly Ser Arg Phe Asp Leu Asn Asp Val
20 25 30
Tyr Val Tyr Trp Gln Thr Ser Glu Ser Lys Thr Val Val Thr Tyr His
35 40 45
Ile Pro Gln Asn Ser Ser Leu Glu Asn Val Asp Ser Arg Tyr Arg Asn
50 55 60
Arg Ala Leu Met Ser Pro Ala Gly Met Leu Arg Gly Asp Phe Ser Leu
65 70 75 80
Arg Leu Phe Asn Val Thr Pro Gln Asp Glu Gln Lys Phe His Cys Leu
85 90 95
Val Leu Ser Gln Ser Leu Gly Phe Gln Glu Val Leu Ser Val Glu Val
100 105 110
Thr Leu His Val Ala Ala Asn Phe Ser Val Pro Val Val Ser Ala Pro
115 120 125
His Ser Pro Ser Gln Asp Glu Leu Thr Phe Thr Cys Thr Ser Ile Asn
130 135 140
Gly Tyr Pro Arg Pro Asn Val Tyr Trp Ile Asn Lys Thr Asp Asn Ser
145 150 155 160
Leu Leu Asp Gln Ala Leu Gln Asn Asp Thr Val Phe Leu Asn Met Arg
165 170 175
Gly Leu Tyr Asp Val Val Ser Val Leu Arg Ile Ala Arg Thr Pro Ser
180 185 190
Val Asn Ile Gly Cys Cys Ile Glu Asn Val Leu Leu Gln Gln Asn Leu
195 200 205
Thr Val Gly Ser Gln Thr Gly Asn Asp Ile Gly Glu Arg Asp Lys Ile
210 215 220
Thr Glu Asn Pro Val Ser Thr Gly Glu Lys Asn Ala Ala Thr
225 230 235
<210> 335
<211> 249
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 335
Tyr Phe Arg Ala Gln Met Asp Pro Asn Arg Ile Ser Glu Asp Gly Thr
1 5 10 15
His Cys Ile Tyr Arg Ile Leu Arg Leu His Glu Asn Ala Asp Phe Gln
20 25 30
Asp Thr Thr Leu Glu Ser Gln Asp Thr Lys Leu Ile Pro Asp Ser Cys
35 40 45
Arg Arg Ile Lys Gln Ala Phe Gln Gly Ala Val Gln Lys Glu Leu Gln
50 55 60
His Ile Val Gly Ser Gln His Ile Arg Ala Glu Lys Ala Met Val Asp
65 70 75 80
Gly Ser Trp Leu Asp Leu Ala Lys Arg Ser Lys Leu Glu Ala Gln Pro
85 90 95
Phe Ala His Leu Thr Ile Asn Ala Thr Asp Ile Pro Ser Gly Ser His
100 105 110
Lys Val Ser Leu Ser Ser Trp Tyr His Asp Arg Gly Trp Ala Lys Ile
115 120 125
Ser Asn Met Thr Phe Ser Asn Gly Lys Leu Ile Val Asn Gln Asp Gly
130 135 140
Phe Tyr Tyr Leu Tyr Ala Asn Ile Cys Phe Arg His His Glu Thr Ser
145 150 155 160
Gly Asp Leu Ala Thr Glu Tyr Leu Gln Leu Met Val Tyr Val Thr Lys
165 170 175
Thr Ser Ile Lys Ile Pro Ser Ser His Thr Leu Met Lys Gly Gly Ser
180 185 190
Thr Lys Tyr Trp Ser Gly Asn Ser Glu Phe His Phe Tyr Ser Ile Asn
195 200 205
Val Gly Gly Phe Phe Lys Leu Arg Ser Gly Glu Glu Ile Ser Ile Glu
210 215 220
Val Ser Asn Pro Ser Leu Leu Asp Pro Asp Gln Asp Ala Thr Tyr Phe
225 230 235 240
Gly Ala Phe Lys Val Arg Asp Ile Asp
245
<210> 336
<211> 182
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 336
Leu Gln Leu His Trp Arg Leu Gly Glu Met Val Thr Arg Leu Pro Asp
1 5 10 15
Gly Pro Ala Gly Ser Trp Glu Gln Leu Ile Gln Glu Arg Arg Ser His
20 25 30
Glu Val Asn Pro Ala Ala His Leu Thr Gly Ala Asn Ser Ser Leu Thr
35 40 45
Gly Ser Gly Gly Pro Leu Leu Trp Glu Thr Gln Leu Gly Leu Ala Phe
50 55 60
Leu Arg Gly Leu Ser Tyr His Asp Gly Ala Leu Val Val Thr Lys Ala
65 70 75 80
Gly Tyr Tyr Tyr Ile Tyr Ser Lys Val Gln Leu Gly Gly Val Gly Cys
85 90 95
Pro Leu Gly Leu Ala Ser Thr Ile Thr His Gly Leu Tyr Lys Arg Thr
100 105 110
Pro Arg Tyr Pro Glu Glu Leu Glu Leu Leu Val Ser Gln Gln Ser Pro
115 120 125
Cys Gly Arg Ala Thr Ser Ser Ser Arg Val Trp Trp Asp Ser Ser Phe
130 135 140
Leu Gly Gly Val Val His Leu Glu Ala Gly Glu Lys Val Val Val Arg
145 150 155 160
Val Leu Asp Glu Arg Leu Val Arg Leu Arg Asp Gly Thr Arg Ser Tyr
165 170 175
Phe Gly Ala Phe Met Val
180
<210> 337
<211> 218
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 337
Gln Val Ala Ala Leu Gln Gly Asp Leu Ala Ser Leu Arg Ala Glu Leu
1 5 10 15
Gln Gly His His Ala Glu Lys Leu Pro Ala Gly Ala Gly Ala Pro Lys
20 25 30
Ala Gly Leu Glu Glu Ala Pro Ala Val Thr Ala Gly Leu Lys Ile Phe
35 40 45
Glu Pro Pro Ala Pro Gly Glu Gly Asn Ser Ser Gln Asn Ser Arg Asn
50 55 60
Lys Arg Ala Val Gln Gly Pro Glu Glu Thr Val Thr Gln Asp Cys Leu
65 70 75 80
Gln Leu Ile Ala Asp Ser Glu Thr Pro Thr Ile Gln Lys Gly Ser Tyr
85 90 95
Thr Phe Val Pro Trp Leu Leu Ser Phe Lys Arg Gly Ser Ala Leu Glu
100 105 110
Glu Lys Glu Asn Lys Ile Leu Val Lys Glu Thr Gly Tyr Phe Phe Ile
115 120 125
Tyr Gly Gln Val Leu Tyr Thr Asp Lys Thr Tyr Ala Met Gly His Leu
130 135 140
Ile Gln Arg Lys Lys Val His Val Phe Gly Asp Glu Leu Ser Leu Val
145 150 155 160
Thr Leu Phe Arg Cys Ile Gln Asn Met Pro Glu Thr Leu Pro Asn Asn
165 170 175
Ser Cys Tyr Ser Ala Gly Ile Ala Lys Leu Glu Glu Gly Asp Glu Leu
180 185 190
Gln Leu Ala Ile Pro Arg Glu Asn Ala Gln Ile Ser Leu Asp Gly Asp
195 200 205
Val Thr Phe Phe Gly Ala Leu Lys Leu Leu
210 215
<210> 338
<211> 224
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 338
Ala Pro Leu Lys Ile Gln Ala Tyr Phe Asn Glu Thr Ala Asp Leu Pro
1 5 10 15
Cys Gln Phe Ala Asn Ser Gln Asn Gln Ser Leu Ser Glu Leu Val Val
20 25 30
Phe Trp Gln Asp Gln Glu Asn Leu Val Leu Asn Glu Val Tyr Leu Gly
35 40 45
Lys Glu Lys Phe Asp Ser Val His Ser Lys Tyr Met Gly Arg Thr Ser
50 55 60
Phe Asp Ser Asp Ser Trp Thr Leu Arg Leu His Asn Leu Gln Ile Lys
65 70 75 80
Asp Lys Gly Leu Tyr Gln Cys Ile Ile His His Lys Lys Pro Thr Gly
85 90 95
Met Ile Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn
100 105 110
Phe Ser Gln Pro Glu Ile Val Pro Ile Ser Asn Ile Thr Glu Asn Val
115 120 125
Tyr Ile Asn Leu Thr Cys Ser Ser Ile His Gly Tyr Pro Glu Pro Lys
130 135 140
Lys Met Ser Val Leu Leu Arg Thr Lys Asn Ser Thr Ile Glu Tyr Asp
145 150 155 160
Gly Val Met Gln Lys Ser Gln Asp Asn Val Thr Glu Leu Tyr Asp Val
165 170 175
Ser Ile Ser Leu Ser Val Ser Phe Pro Asp Val Thr Ser Asn Met Thr
180 185 190
Ile Phe Cys Ile Leu Glu Thr Asp Lys Thr Arg Leu Leu Ser Ser Pro
195 200 205
Phe Ser Ile Glu Leu Glu Asp Pro Gln Pro Pro Pro Asp His Ile Pro
210 215 220
<210> 339
<211> 208
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 339
Val Ile His Val Thr Lys Glu Val Lys Glu Val Ala Thr Leu Ser Cys
1 5 10 15
Gly His Asn Val Ser Val Glu Glu Leu Ala Gln Thr Arg Ile Tyr Trp
20 25 30
Gln Lys Glu Lys Lys Met Val Leu Thr Met Met Ser Gly Asp Met Asn
35 40 45
Ile Trp Pro Glu Tyr Lys Asn Arg Thr Ile Phe Asp Ile Thr Asn Asn
50 55 60
Leu Ser Ile Val Ile Leu Ala Leu Arg Pro Ser Asp Glu Gly Thr Tyr
65 70 75 80
Glu Cys Val Val Leu Lys Tyr Glu Lys Asp Ala Phe Lys Arg Glu His
85 90 95
Leu Ala Glu Val Thr Leu Ser Val Lys Ala Asp Phe Pro Thr Pro Ser
100 105 110
Ile Ser Asp Phe Glu Ile Pro Thr Ser Asn Ile Arg Arg Ile Ile Cys
115 120 125
Ser Thr Ser Gly Gly Phe Pro Glu Pro His Leu Ser Trp Leu Glu Asn
130 135 140
Gly Glu Glu Leu Asn Ala Ile Asn Thr Thr Val Ser Gln Asp Pro Glu
145 150 155 160
Thr Glu Leu Tyr Ala Val Ser Ser Lys Leu Asp Phe Asn Met Thr Thr
165 170 175
Asn His Ser Phe Met Cys Leu Ile Lys Tyr Gly His Leu Arg Val Asn
180 185 190
Gln Thr Phe Asn Trp Asn Thr Thr Lys Gln Glu His Phe Pro Asp Asn
195 200 205
<210> 340
<211> 249
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 340
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
1 5 10 15
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
20 25 30
Tyr Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile
35 40 45
Gly Cys Ile Tyr Pro Gly Asn Val Asn Thr Asn Tyr Asn Glu Lys Phe
50 55 60
Lys Asp Arg Ala Thr Leu Thr Val Asp Thr Ser Ile Ser Thr Ala Tyr
65 70 75 80
Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Phe Cys
85 90 95
Thr Arg Ser His Tyr Gly Leu Asp Trp Asn Phe Asp Val Trp Gly Gln
100 105 110
Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
115 120 125
Gly Ser Gly Gly Gly Gly Ser Gly Gly Asp Ile Gln Met Thr Gln Ser
130 135 140
Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys
145 150 155 160
His Ala Ser Gln Asn Ile Tyr Val Trp Leu Asn Trp Tyr Gln Gln Lys
165 170 175
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Lys Ala Ser Asn Leu His
180 185 190
Thr Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe
195 200 205
Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr
210 215 220
Cys Gln Gln Gly Gln Thr Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys
225 230 235 240
Val Glu Ile Lys Gly Gly Gly Gly Ser
245
<210> 341
<211> 42
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 341
Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His
1 5 10 15
Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln
20 25 30
Ala Asp Ala His Ser Thr Leu Ala Lys Ile
35 40
<210> 342
<211> 62
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 342
Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His Pro Lys Gln
1 5 10 15
Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly Ser Asn Thr
20 25 30
Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro Val Thr Gln
35 40 45
Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg Gln
50 55 60
<210> 343
<211> 48
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 343
Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro
1 5 10 15
Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr
20 25 30
Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro
35 40 45
<210> 344
<211> 42
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 344
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
1 5 10 15
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
20 25 30
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
35 40
<210> 345
<211> 383
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 345
Cys Tyr Arg Lys Lys Gly Lys Ala Leu Thr Ala Asn Leu Trp His Trp
1 5 10 15
Ile Asn Glu Ala Cys Gly Arg Leu Ser Gly Asp Lys Glu Ser Ser Gly
20 25 30
Asp Ser Cys Val Ser Thr His Thr Ala Asn Phe Gly Gln Gln Gly Ala
35 40 45
Cys Glu Gly Val Leu Leu Leu Thr Leu Glu Glu Lys Thr Phe Pro Glu
50 55 60
Asp Met Cys Tyr Pro Asp Gln Gly Gly Val Cys Gln Gly Thr Cys Val
65 70 75 80
Gly Gly Gly Pro Tyr Ala Gln Gly Glu Asp Ala Arg Met Leu Ser Leu
85 90 95
Val Ser Lys Thr Glu Ile Glu Glu Asp Ser Phe Arg Gln Met Pro Thr
100 105 110
Glu Asp Glu Tyr Met Asp Arg Pro Ser Gln Pro Thr Asp Gln Leu Leu
115 120 125
Phe Leu Thr Glu Pro Gly Ser Lys Ser Thr Pro Pro Phe Ser Glu Pro
130 135 140
Leu Glu Val Gly Glu Asn Asp Ser Leu Ser Gln Cys Phe Thr Gly Thr
145 150 155 160
Gln Ser Thr Val Gly Ser Glu Ser Cys Asn Cys Thr Glu Pro Leu Cys
165 170 175
Arg Thr Asp Trp Thr Pro Met Ser Ser Glu Asn Tyr Leu Gln Lys Glu
180 185 190
Val Asp Ser Gly His Cys Pro His Trp Ala Ala Ser Pro Ser Pro Asn
195 200 205
Trp Ala Asp Val Cys Thr Gly Cys Arg Asn Pro Pro Gly Glu Asp Cys
210 215 220
Glu Pro Leu Val Gly Ser Pro Lys Arg Gly Pro Leu Pro Gln Cys Ala
225 230 235 240
Tyr Gly Met Gly Leu Pro Pro Glu Glu Glu Ala Ser Arg Thr Glu Ala
245 250 255
Arg Asp Gln Pro Glu Asp Gly Ala Asp Gly Arg Leu Pro Ser Ser Ala
260 265 270
Arg Ala Gly Ala Gly Ser Gly Ser Ser Pro Gly Gly Gln Ser Pro Ala
275 280 285
Ser Gly Asn Val Thr Gly Asn Ser Asn Ser Thr Phe Ile Ser Ser Gly
290 295 300
Gln Val Met Asn Phe Lys Gly Asp Ile Ile Val Val Tyr Val Ser Gln
305 310 315 320
Thr Ser Gln Glu Gly Ala Ala Ala Ala Ala Glu Pro Met Gly Arg Pro
325 330 335
Val Gln Glu Glu Thr Leu Ala Arg Arg Asp Ser Phe Ala Gly Asn Gly
340 345 350
Pro Arg Phe Pro Asp Pro Cys Gly Gly Pro Glu Gly Leu Arg Glu Pro
355 360 365
Glu Lys Ala Ser Arg Pro Val Gln Glu Gln Gly Gly Ala Lys Ala
370 375 380
<210> 346
<211> 107
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 346
Lys Lys Arg Gly Asp Pro Cys Ser Cys Gln Pro Arg Ser Arg Pro Arg
1 5 10 15
Gln Ser Pro Ala Lys Ser Ser Gln Asp His Ala Met Glu Ala Gly Ser
20 25 30
Pro Val Ser Thr Ser Pro Glu Pro Val Glu Thr Cys Ser Phe Cys Phe
35 40 45
Pro Glu Cys Arg Ala Pro Thr Gln Glu Ser Ala Val Thr Pro Gly Thr
50 55 60
Pro Asp Pro Thr Cys Ala Gly Arg Trp Gly Cys His Thr Arg Thr Thr
65 70 75 80
Val Leu Gln Pro Cys Pro His Ile Pro Asp Ser Gly Leu Gly Ile Val
85 90 95
Cys Val Pro Ala Gln Glu Gly Gly Pro Gly Ala
100 105
<210> 347
<211> 85
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 347
Ser Trp Arg Arg Arg Gln Arg Arg Leu Arg Gly Ala Ser Ser Ala Glu
1 5 10 15
Ala Pro Asp Gly Asp Lys Asp Ala Pro Glu Pro Leu Asp Lys Val Ile
20 25 30
Ile Leu Ser Pro Gly Ile Ser Asp Ala Thr Ala Pro Ala Trp Pro Pro
35 40 45
Pro Gly Glu Asp Pro Gly Thr Thr Pro Pro Gly His Ser Val Pro Val
50 55 60
Pro Ala Thr Glu Leu Gly Ser Thr Glu Leu Val Thr Thr Lys Thr Ala
65 70 75 80
Gly Pro Glu Gln Gln
85
<210> 348
<211> 60
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 348
Cys Val Lys Arg Arg Lys Pro Arg Gly Asp Val Val Lys Val Ile Val
1 5 10 15
Ser Val Gln Arg Lys Arg Gln Glu Ala Glu Gly Glu Ala Thr Val Ile
20 25 30
Glu Ala Leu Gln Ala Pro Pro Asp Val Thr Thr Val Ala Val Glu Glu
35 40 45
Thr Ile Pro Ser Phe Thr Gly Arg Ser Pro Asn His
50 55 60
<210> 349
<211> 107
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 349
Arg Lys Ile Asn Ser Glu Pro Leu Lys Asp Glu Phe Lys Asn Thr Gly
1 5 10 15
Ser Gly Leu Leu Gly Met Ala Asn Ile Asp Leu Glu Lys Ser Arg Thr
20 25 30
Gly Asp Glu Ile Ile Leu Pro Arg Gly Leu Glu Tyr Thr Val Glu Glu
35 40 45
Cys Thr Cys Glu Asp Cys Ile Lys Ser Lys Pro Lys Val Asp Ser Asp
50 55 60
His Cys Phe Pro Leu Pro Ala Met Glu Glu Gly Ala Thr Ile Leu Val
65 70 75 80
Thr Thr Lys Thr Asn Asp Tyr Cys Lys Ser Leu Pro Ala Ala Leu Ser
85 90 95
Ala Thr Glu Ile Glu Lys Ser Ile Ser Ala Arg
100 105
<210> 350
<211> 58
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 350
Gln Leu Gly Leu His Ile Trp Gln Leu Arg Ser Gln Cys Met Trp Pro
1 5 10 15
Arg Glu Thr Gln Leu Leu Leu Glu Val Pro Pro Ser Thr Glu Asp Ala
20 25 30
Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu
35 40 45
Glu Lys Gly Arg Leu Gly Asp Leu Trp Val
50 55
<210> 351
<211> 41
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 351
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr
1 5 10 15
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
20 25 30
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
35 40
<210> 352
<211> 41
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 352
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Pro
1 5 10 15
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
20 25 30
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
35 40
<210> 353
<211> 111
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 353
Phe Trp Gly Arg Arg Ser Cys Gln Gln Arg Asp Ser Gly Asn Ser Pro
1 5 10 15
Gly Asn Ala Phe Tyr Ser Asn Val Leu Tyr Arg Pro Arg Gly Ala Pro
20 25 30
Lys Lys Ser Glu Asp Cys Ser Gly Glu Gly Lys Asp Gln Arg Gly Gln
35 40 45
Ser Ile Tyr Ser Thr Ser Phe Pro Gln Pro Ala Pro Arg Gln Pro His
50 55 60
Leu Ala Ser Arg Pro Cys Pro Ser Pro Arg Pro Cys Pro Ser Pro Arg
65 70 75 80
Pro Gly His Pro Val Ser Met Val Arg Val Ser Pro Arg Pro Ser Pro
85 90 95
Thr Gln Gln Pro Arg Pro Lys Gly Phe Pro Lys Val Gly Glu Glu
100 105 110
<210> 354
<211> 38
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 354
Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn
1 5 10 15
Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg
20 25 30
Leu Thr Asp Val Thr Leu
35
<210> 355
<211> 61
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 355
Asn Arg Arg Arg Arg Arg Glu Arg Arg Asp Leu Phe Thr Glu Ser Trp
1 5 10 15
Asp Thr Gln Lys Ala Pro Asn Asn Tyr Arg Ser Pro Ile Ser Thr Ser
20 25 30
Gln Pro Thr Asn Gln Ser Met Asp Asp Thr Arg Glu Asp Ile Tyr Val
35 40 45
Asn Tyr Pro Thr Phe Ser Arg Arg Pro Lys Thr Arg Val
50 55 60
<210> 356
<211> 77
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 356
Gln Leu Arg Arg Arg Gly Lys Thr Asn His Tyr Gln Thr Thr Val Glu
1 5 10 15
Lys Lys Ser Leu Thr Ile Tyr Ala Gln Val Gln Lys Pro Gly Pro Leu
20 25 30
Gln Lys Lys Leu Asp Ser Phe Pro Ala Gln Asp Pro Cys Thr Thr Ile
35 40 45
Tyr Val Ala Ala Thr Glu Pro Val Pro Glu Ser Val Gln Glu Thr Asn
50 55 60
Ser Ile Thr Val Tyr Ala Ser Val Thr Leu Pro Glu Ser
65 70 75
<210> 357
<211> 48
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 357
Lys Lys Tyr Phe Phe Lys Lys Glu Val Gln Gln Leu Ser Val Ser Phe
1 5 10 15
Ser Ser Leu Gln Ile Lys Ala Leu Gln Asn Ala Val Glu Lys Glu Val
20 25 30
Gln Ala Glu Asp Asn Ile Tyr Ile Glu Asn Ser Leu Tyr Ala Thr Asp
35 40 45
<210> 358
<211> 100
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 358
Lys Val Phe Leu Arg Cys Ile Asn Tyr Val Phe Phe Pro Ser Leu Lys
1 5 10 15
Pro Ser Ser Ser Ile Asp Glu Tyr Phe Ser Glu Gln Pro Leu Lys Asn
20 25 30
Leu Leu Leu Ser Thr Ser Glu Glu Gln Ile Glu Lys Cys Phe Ile Ile
35 40 45
Glu Asn Ile Ser Thr Ile Ala Thr Val Glu Glu Thr Asn Gln Thr Asp
50 55 60
Glu Asp His Lys Lys Tyr Ser Ser Gln Thr Ser Gln Asp Ser Gly Asn
65 70 75 80
Tyr Ser Asn Glu Asp Glu Ser Glu Ser Lys Thr Ser Glu Glu Leu Gln
85 90 95
Gln Asp Phe Val
100
<210> 359
<211> 251
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 359
Lys Trp Ile Gly Tyr Ile Cys Leu Arg Asn Ser Leu Pro Lys Val Leu
1 5 10 15
Asn Phe His Asn Phe Leu Ala Trp Pro Phe Pro Asn Leu Pro Pro Leu
20 25 30
Glu Ala Met Asp Met Val Glu Val Ile Tyr Ile Asn Arg Lys Lys Lys
35 40 45
Val Trp Asp Tyr Asn Tyr Asp Asp Glu Ser Asp Ser Asp Thr Glu Ala
50 55 60
Ala Pro Arg Thr Ser Gly Gly Gly Tyr Thr Met His Gly Leu Thr Val
65 70 75 80
Arg Pro Leu Gly Gln Ala Ser Ala Thr Ser Thr Glu Ser Gln Leu Ile
85 90 95
Asp Pro Glu Ser Glu Glu Glu Pro Asp Leu Pro Glu Val Asp Val Glu
100 105 110
Leu Pro Thr Met Pro Lys Asp Ser Pro Gln Gln Leu Glu Leu Leu Ser
115 120 125
Gly Pro Cys Glu Arg Arg Lys Ser Pro Leu Gln Asp Pro Phe Pro Glu
130 135 140
Glu Asp Tyr Ser Ser Thr Glu Gly Ser Gly Gly Arg Ile Thr Phe Asn
145 150 155 160
Val Asp Leu Asn Ser Val Phe Leu Arg Val Leu Asp Asp Glu Asp Ser
165 170 175
Asp Asp Leu Glu Ala Pro Leu Met Leu Ser Ser His Leu Glu Glu Met
180 185 190
Val Asp Pro Glu Asp Pro Asp Asn Val Gln Ser Asn His Leu Leu Ala
195 200 205
Ser Gly Glu Gly Thr Gln Pro Thr Phe Pro Ser Pro Ser Ser Glu Gly
210 215 220
Leu Trp Ser Glu Asp Ala Pro Ser Asp Gln Ser Asp Thr Ser Glu Ser
225 230 235 240
Asp Val Asp Leu Gly Asp Gly Tyr Ile Met Arg
245 250
<210> 360
<211> 286
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 360
Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn
1 5 10 15
Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly
20 25 30
Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe
35 40 45
Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu
50 55 60
Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu
65 70 75 80
Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn
85 90 95
Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala
100 105 110
Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp
115 120 125
Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln
130 135 140
Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp
145 150 155 160
Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro
165 170 175
Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro
180 185 190
Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly
195 200 205
Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro
210 215 220
Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro
225 230 235 240
Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu
245 250 255
Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu
260 265 270
Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val
275 280 285
<210> 361
<211> 86
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 361
Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu
1 5 10 15
Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys
20 25 30
Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu
35 40 45
Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly
50 55 60
Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr
65 70 75 80
Thr Leu Lys Pro Glu Thr
85
<210> 362
<211> 36
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 362
Glu Glu Ser Val Val Arg Pro Ser Val Phe Val Val Asp Gly Gln Thr
1 5 10 15
Asp Ile Pro Phe Thr Arg Leu Gly Arg Ser His Arg Arg Gln Ser Cys
20 25 30
Ser Val Ala Arg
35
<210> 363
<211> 50
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 363
Gly Trp Ile Arg Gly Arg Arg Ser Arg His Ser Trp Glu Met Ser Glu
1 5 10 15
Phe His Asn Tyr Asn Leu Asp Leu Lys Lys Ser Asp Phe Ser Thr Arg
20 25 30
Trp Gln Lys Gln Arg Cys Pro Val Val Lys Ser Lys Cys Arg Glu Asn
35 40 45
Ala Ser
50
<210> 364
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 364
Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly Leu
1 5 10 15
Pro Ile Ser Met Lys
20
<210> 365
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 365
Phe Leu Phe Val Leu Leu Gly Val Gly Ser Met Gly Val Ala Ala Ile
1 5 10 15
Val Trp Gly Ala Trp
20
<210> 366
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 366
Phe Trp Leu Pro Ile Gly Cys Ala Ala Phe Val Val Val Cys Ile Leu
1 5 10 15
Gly Cys Ile Leu Ile
20
<210> 367
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 367
Ile Leu Val Ile Phe Ser Gly Met Phe Leu Val Phe Thr Leu Ala Gly
1 5 10 15
Ala Leu Phe Leu His
20
<210> 368
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 368
Gly Gly Thr Val Leu Leu Leu Leu Phe Val Ile Ser Ile Thr Thr Ile
1 5 10 15
Ile Val Ile Phe Leu
20
<210> 369
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 369
Trp Ala Val Tyr Ala Gly Leu Leu Gly Gly Val Ile Met Ile Leu Ile
1 5 10 15
Met Val Val Ile Leu
20
<210> 370
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 370
Ile Tyr Ala Gly Val Cys Ile Ser Val Leu Val Leu Leu Ala Leu Leu
1 5 10 15
Gly Val Ile Ile Ala
20
<210> 371
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 371
Ile Trp Leu Ile Val Gly Ile Cys Ile Ala Leu Phe Ala Leu Pro Phe
1 5 10 15
Val Ile Tyr Ala Ala
20
<210> 372
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 372
Ile Gly Gly Ile Ile Thr Val Phe Leu Ile Ala Leu Val Leu Thr Ser
1 5 10 15
Thr Ile Val Thr Leu
20
<210> 373
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 373
Val Ala Ala Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly Pro
1 5 10 15
Leu Ala Ile Leu Leu
20
<210> 374
<211> 22
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 374
Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu
1 5 10 15
Leu Val Leu Val Phe Ile
20
<210> 375
<211> 27
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 375
Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu
1 5 10 15
Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val
20 25
<210> 376
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 376
Gly Leu Ile Ile Leu Leu Leu Phe Ala Ser Val Ala Leu Val Ala Ala
1 5 10 15
Ile Ile Phe Gly Val
20
<210> 377
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 377
Thr Leu Gly Leu Cys Leu Cys Ala Val Leu Cys Cys Phe Leu Val Ala
1 5 10 15
Val Ala Cys Phe Leu
20
<210> 378
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 378
Phe Gly Ala Pro Ala Leu Leu Gly Leu Ala Leu Val Leu Ala Leu Val
1 5 10 15
Leu Val Gly Leu Val
20
<210> 379
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 379
Trp Trp Phe Leu Ser Gly Ser Leu Val Ile Val Ile Val Cys Ser Thr
1 5 10 15
Val Gly Leu Ile Ile
20
<210> 380
<211> 23
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 380
Ile Leu Trp Thr Cys Leu Gly Leu Ser Leu Ile Ile Ser Leu Ala Val
1 5 10 15
Phe Val Leu Met Phe Leu Leu
20
<210> 381
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 381
Leu Gly Trp Leu Thr Val Val Leu Leu Ala Val Ala Ala Cys Val Leu
1 5 10 15
Leu Leu Thr Ser Ala
20
<210> 382
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 382
Pro Phe Phe Phe Cys Cys Phe Ile Ala Val Ala Met Gly Ile Arg Phe
1 5 10 15
Ile Ile Met Val Ala
20
<210> 383
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 383
Val Gly Leu Gly Leu Leu Leu Leu Leu Met Gly Ala Gly Leu Ala Val
1 5 10 15
Gln Gly Trp Phe Leu
20
<210> 384
<211> 24
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 384
Ile Phe Met Tyr Leu Leu Thr Val Phe Leu Ile Thr Gln Met Ile Gly
1 5 10 15
Ser Ala Leu Phe Ala Val Tyr Leu
20
<210> 385
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 385
Phe Leu Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe Tyr
1 5 10 15
Ser Phe Leu Leu Thr
20
<210> 386
<211> 23
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 386
Ile Ala Ala Ile Val Gly Gly Thr Val Ala Gly Ile Val Leu Ile Gly
1 5 10 15
Ile Leu Leu Leu Val Ile Trp
20
<210> 387
<211> 17
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 387
Leu Gly Trp Leu Cys Leu Leu Leu Leu Pro Ile Pro Leu Ile Val Trp
1 5 10 15
Val
<210> 388
<211> 28
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 388
Pro Val Leu Asp Ala Gly Pro Val Leu Phe Trp Val Ile Leu Val Leu
1 5 10 15
Val Val Val Val Gly Ser Ser Ala Phe Leu Leu Cys
20 25
<210> 389
<211> 23
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 389
Ile Trp Val Ile Leu Val Val Thr Leu Val Val Pro Leu Leu Leu Val
1 5 10 15
Ala Val Leu Ile Val Cys Cys
20
<210> 390
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 390
Leu Ser Gly Ile Ile Ile Gly Val Thr Val Ala Ala Val Val Leu Ile
1 5 10 15
Val Ala Val Phe Val
20
<210> 391
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 391
Ala Ala Val Ile Cys Ser Ala Leu Ala Thr Val Leu Leu Ala Leu Leu
1 5 10 15
Ile Leu Cys Val Ile
20
<210> 392
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 392
Leu Pro Trp Met Ile Val Leu Phe Leu Leu Leu Val Leu Val Val Ile
1 5 10 15
Val Val Cys Ser Ile
20
<210> 393
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 393
Met Phe Trp Val Gln Val Leu Leu Ala Gly Leu Val Val Pro Leu Leu
1 5 10 15
Leu Gly Ala Thr Leu
20
<210> 394
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 394
Val Leu Leu Pro Leu Val Ile Phe Phe Gly Leu Cys Leu Leu Ser Leu
1 5 10 15
Leu Phe Ile Gly Leu
20
<210> 395
<211> 27
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 395
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
1 5 10 15
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
20 25
<210> 396
<211> 21
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 396
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu
1 5 10 15
Ser Leu Val Ile Thr
20
<210> 397
<211> 328
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 397
cagtgttctt caacctgtgt tccgcggaac cctagggttc cacccaaagg ctttcggggt 60
tccgcgagtc attgcttcaa ttcgagagac gtcggccgcg ccgctcttca gaatgcacat 120
gcgtcaatcg gagtttcatg ttgaaacatg ttatccattc gcatagttga cttacactgc 180
acttaacctt aattttcaaa aatatgtaac tgtacttgtg gtcgtagttt tgttgttgtt 240
ttaggtttag acaagcaaag gtaagttaac ttacagtttt aaaataaatt gtattttgtt 300
tgatcctaac ctagaatcgt tcagaaat 328
<210> 398
<211> 145
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 398
ccaaagcacg ggctcacctt gttcgtaaca agtcaacgca gctgtcccta aaatctcatc 60
tgggtgtatt actaaatgaa gggttccata aaaaaaaata tctcgacaaa gggttccgcc 120
ggatggcaaa ggttgaagaa cactg 145
<210> 399
<211> 16
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 399
cagtgttctt caacct 16
<210> 400
<211> 16
<212> DNA
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 400
aggttgaaga acactg 16
<210> 401
<211> 636
<212> PRT
<213> artificial sequence(人工序列)
<220>
<223> 未知或合成序列
<400> 401
Met Met Leu Asn Trp Leu Lys Ser Gly Lys Leu Glu Ser Gln Ser Gln
1 5 10 15
Glu Gln Ser Ser Cys Tyr Leu Glu Asn Ser Asn Cys Leu Pro Pro Thr
20 25 30
Leu Asp Ser Thr Asp Ile Ile Gly Glu Glu Asn Lys Ala Gly Thr Thr
35 40 45
Ser Arg Lys Lys Arg Lys Tyr Asp Glu Asp Tyr Leu Asn Phe Gly Phe
50 55 60
Thr Trp Thr Gly Asp Lys Asp Glu Pro Asn Gly Leu Cys Val Ile Cys
65 70 75 80
Glu Gln Val Val Asn Asn Ser Ser Leu Asn Pro Ala Lys Leu Lys Arg
85 90 95
His Leu Asp Thr Lys His Pro Thr Leu Lys Gly Lys Ser Glu Tyr Phe
100 105 110
Lys Arg Lys Cys Asn Glu Leu Asn Gln Lys Lys His Thr Phe Glu Arg
115 120 125
Tyr Val Arg Asp Asp Asn Lys Asn Leu Leu Lys Ala Ser Tyr Leu Val
130 135 140
Ser Leu Arg Ile Ala Lys Gln Gly Glu Ala Tyr Thr Ile Ala Glu Lys
145 150 155 160
Leu Ile Lys Pro Cys Thr Lys Asp Leu Thr Thr Cys Val Phe Gly Glu
165 170 175
Lys Phe Ala Ser Lys Val Asp Leu Val Pro Leu Ser Asp Thr Thr Ile
180 185 190
Ser Arg Arg Ile Glu Asp Met Ser Tyr Phe Cys Glu Ala Val Leu Val
195 200 205
Asn Arg Leu Lys Asn Ala Lys Cys Gly Phe Thr Leu Gln Met Asp Glu
210 215 220
Ser Thr Asp Val Ala Gly Leu Ala Ile Leu Leu Val Phe Val Arg Tyr
225 230 235 240
Ile His Glu Ser Ser Phe Glu Glu Asp Met Leu Phe Cys Lys Ala Leu
245 250 255
Pro Thr Gln Thr Thr Gly Glu Glu Ile Phe Asn Leu Leu Asn Ala Tyr
260 265 270
Phe Glu Lys His Ser Ile Pro Trp Asn Leu Cys Tyr His Ile Cys Thr
275 280 285
Asp Gly Ala Lys Ala Met Val Gly Val Ile Lys Gly Val Ile Ala Arg
290 295 300
Ile Lys Lys Leu Val Pro Asp Ile Lys Ala Ser His Cys Cys Leu His
305 310 315 320
Arg His Ala Leu Ala Val Lys Arg Ile Pro Asn Ala Leu His Glu Val
325 330 335
Leu Asn Asp Ala Val Lys Met Ile Asn Phe Ile Lys Ser Arg Pro Leu
340 345 350
Asn Ala Arg Val Phe Ala Leu Leu Cys Asp Asp Leu Gly Ser Leu His
355 360 365
Lys Asn Leu Leu Leu His Thr Glu Val Arg Trp Leu Ser Arg Gly Lys
370 375 380
Val Leu Thr Arg Phe Trp Glu Leu Arg Asp Glu Ile Arg Ile Phe Phe
385 390 395 400
Asn Glu Arg Glu Phe Ala Gly Lys Leu Asn Asp Thr Ser Trp Leu Gln
405 410 415
Asn Leu Ala Tyr Ile Ala Asp Ile Phe Ser Tyr Leu Asn Glu Val Asn
420 425 430
Leu Ser Leu Gln Gly Pro Asn Ser Thr Ile Phe Lys Val Asn Ser Arg
435 440 445
Ile Asn Ser Ile Lys Ser Lys Leu Lys Leu Trp Glu Glu Cys Ile Thr
450 455 460
Lys Asn Asn Thr Glu Cys Phe Ala Asn Leu Asn Asp Phe Leu Glu Thr
465 470 475 480
Ser Asn Thr Ala Leu Asp Pro Asn Leu Lys Ser Asn Ile Leu Glu His
485 490 495
Leu Asn Gly Leu Lys Asn Thr Phe Leu Glu Tyr Phe Pro Pro Thr Cys
500 505 510
Asn Asn Ile Ser Trp Val Glu Asn Pro Phe Asn Glu Cys Gly Asn Val
515 520 525
Asp Thr Leu Pro Ile Lys Glu Arg Glu Gln Leu Ile Asp Ile Arg Thr
530 535 540
Asp Thr Thr Leu Lys Ser Ser Phe Val Pro Asp Gly Ile Gly Pro Phe
545 550 555 560
Trp Ile Lys Leu Met Asp Glu Phe Pro Glu Ile Ser Lys Arg Ala Val
565 570 575
Lys Glu Leu Met Pro Phe Val Thr Thr Tyr Leu Cys Glu Lys Ser Phe
580 585 590
Ser Val Tyr Val Ala Thr Lys Thr Lys Tyr Arg Asn Arg Leu Asp Ala
595 600 605
Glu Asp Asp Met Arg Leu Gln Leu Thr Thr Ile His Pro Asp Ile Asp
610 615 620
Asn Leu Cys Asn Asn Lys Gln Ala Gln Lys Ser His
625 630 635

Claims (59)

1.一种包含免疫细胞存活增强基因的多核苷酸，所述免疫细胞存活增强基因包含与异源调控序列可操作地连接的编码蛋白质的核酸，所述异源调控序列对所述蛋白质在免疫细胞内的表达有效，从而增强所述免疫细胞的存活。
2.根据权利要求1所述的多核苷酸，其中所述免疫细胞存活增强基因编码包含激活突变的天然存在的蛋白质。
3.根据权利要求2所述的多核苷酸，其中所述免疫细胞存活增强基因编码选自包含激活突变的STAT3、CD28、RhoA、PLCG、STAT5B或CCND1的蛋白质。
4.根据权利要求3所述的多核苷酸，其中所述免疫细胞存活增强基因编码STAT3，其中所述STAT3包含以下激活突变中的一个或多个：F174S、H410R、S614R、E616K、G618R、Y640F、N647I、E652K、K658Y、K658R、K658N、K658M、K658R、K658H、K658N、D661Y或D661V。
5.根据权利要求3所述的多核苷酸，其中所述免疫细胞存活增强基因编码CD28，其中所述CD28包含以下激活突变中的一个或多个：D124E、D124V、T195I或T195P。
6.根据权利要求3所述的多核苷酸，其中所述免疫细胞存活增强基因编码RhoA，其中所述RhoA包含以下激活突变中的一个或多个：G17V或K18N。
7.根据权利要求3所述的多核苷酸，其中所述免疫细胞存活增强基因编码PLCG，其中所述PLCG包含以下激活突变中的一个或多个：S345F、S520F或R707Q。
8.根据权利要求3所述的多核苷酸，其中所述免疫细胞存活增强基因编码STAT5B，其中所述STAT5B包含以下激活突变中的一个或多个：N642H、T648S、S652Y、Y665F或P267A。
9.根据权利要求3所述的多核苷酸，其中所述免疫细胞存活增强基因编码CCND1，其中所述CCND1包含以下激活突变中的一个或多个：E36G、E36Q、E36K、A39S、S41L、S41P、S41T、V42E、V42A、V42L、V42M、Y44S、Y44D、Y44C、Y44H、K46T、K46R、K46N、K46E、C47G、C47R、C47S、C47W、P199R、P199S、P199L、S201F、T285I、T285A、P286L、P286H、P286S、P286T或P286A。
10.根据权利要求1所述的多核苷酸，其中所述免疫细胞存活增强基因编码天然存在的人蛋白质。
11.根据权利要求10所述的多核苷酸，其中所述免疫细胞存活增强基因编码选自生存素(Survivin)、Bcl2、Bcl6或Bcl-XL的蛋白质。
12.根据权利要求1所述的多核苷酸，其中所述免疫细胞存活增强基因编码凋亡抑制因子。
13.根据权利要求1所述的多核苷酸，其中所述异源启动子选自EF1启动子、PGK启动子、GAPDH启动子、EEF2启动子、泛素启动子、SV40启动子或HSVTK启动子。
14.根据权利要求12所述的多核苷酸物，其中所述异源启动子选自SEQ ID No：94-154。
15.根据权利要求1所述的多核苷酸，其中所述多核苷酸还包含选自SEQ ID NO：6和7、或SEQ ID NO：14和15、或SEQ ID NO：18和19、或SEQ ID NO：20和21、或SEQ ID NO：26和27、或SEQ ID NO：399和400的序列对。
16.根据权利要求1所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的半衰期，所述基因组包含所述多核苷酸的免疫细胞的半衰期增加至少25％。
17.根据权利要求1所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的最大寿命，所述基因组包含所述多核苷酸的免疫细胞的最大寿命增加至少25％。
18.根据权利要求1所述的多核苷酸，其中相对于基因组包含所述多核苷酸的免疫细胞的倍增时间，所述基因组不包含所述多核苷酸的免疫细胞的倍增时间高出至少25％。
19.根据权利要求1所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的增殖速率，所述基因组包含所述多核苷酸的免疫细胞的增殖速率增加至少25％。
20.根据权利要求1所述的多核苷酸，其中相对于基因组不包含所述多核苷酸的免疫细胞的存活率，所述基因组包含所述多核苷酸的T细胞在重复抗原激发后的存活率增加至少25％。
21.一种转座子，其包含前述权利要求中任一项所述的多核苷酸。
22.一种慢病毒载体，其包含权利要求1-20中任一项所述的多核苷酸。
23.一种产生修饰的免疫细胞的方法，所述方法包括将与异源启动子可操作地连接的编码凋亡抑制因子的多核苷酸导入免疫细胞。
24.根据权利要求23所述的方法，其中所述多核苷酸还包含转座子末端，并且其中所述方法还包括将相应的转座酶导入所述免疫细胞，使得编码所述凋亡抑制因子的多核苷酸被转座到所述免疫细胞的基因组中。
25.根据权利要求24所述的方法，其中所述转座酶作为编码转座酶的核酸被导入。
26.根据权利要求25所述的方法，其中所述核酸为mRNA。
27.根据权利要求25所述的方法，其中编码所述转座酶的所述核酸与在所述免疫细胞中有活性的启动子可操作地连接。
28.根据权利要求24所述的方法，其中将所述转座子和转座酶同时导入所述免疫细胞中。
29.根据权利要求24所述的方法，其中将所述转座子和转座酶在不同时间导入所述免疫细胞中。
30.根据权利要求23所述的方法，其中所述免疫细胞为T细胞，所述方法还包括将编码能够结合抗原的受体的基因导入所述免疫细胞，其中所述受体与在其表面展示所述抗原的靶细胞结合导致所述T细胞杀伤所述靶细胞。
31.根据权利要求23所述的方法，其中所述凋亡抑制因子选自生存素、Bcl2、Bcl6、Bcl-XL或Casp3、Casp7、Casp8、Casp9或Casp10的显性失活突变体。
32.一种产生修饰的免疫细胞的方法，所述方法包括将编码选自STAT3、CD28、RhoA、PLCG、STAT5B或CCND1的蛋白质的多核苷酸导入免疫细胞，其中所述蛋白质包含与异源启动子可操作地连接的激活突变。
33.一种产生修饰的免疫细胞的方法，所述方法包括将编码多肽的多核苷酸导入免疫细胞，所述多肽包含
a.衍生自通常向免疫细胞传递抑制信号的受体的胞外结构域的序列
b.衍生自向免疫细胞传递刺激信号的受体的胞内结构域的序列
c.跨膜结构域
并且其中所述多肽不包含CD3ζ胞内结构域。
34.根据权利要求33所述的方法，其中所述胞外结构域包含选自SEQ ID NO：322-340的序列。
35.根据权利要求33所述的方法，其中所述胞内结构域包含选自SEQ ID NO：341-364的序列。
36.根据权利要求33所述的方法，其中所述多肽包含选自SEQ ID NO：274-318的序列。
37.一种免疫细胞，其基因组包含权利要求1所述的多核苷酸。
38.根据权利要求37所述的免疫细胞，其中相对于基因组不包含权利要求1所述的多核苷酸的免疫细胞的半衰期，所述免疫细胞的半衰期增加至少25％。
39.根据权利要求37所述的免疫细胞，其中相对于基因组不包含权利要求1所述的多核苷酸的免疫细胞的最大寿命，所述免疫细胞的最大寿命增加至少25％。
40.根据权利要求37所述的免疫细胞，其中相对于基因组包含权利要求1所述的多核苷酸的免疫细胞的半衰期，所述基因组不包含根据权利要求1所述的多核苷酸的免疫细胞的倍增时间增加至少25％。
41.根据权利要求37所述的免疫细胞，其中相对于基因组不包含权利要求1所述的多核苷酸的免疫细胞的增殖速率，所述免疫细胞的增殖速率增加至少25％。
42.根据权利要求37所述的免疫细胞，其中相对于基因组不包含所述多核苷酸的免疫细胞的存活率，所述基因组包含所述多核苷酸的T细胞在重复抗原激发后的存活率增加至少25％。
43.根据权利要求37所述的免疫细胞，其中所述免疫细胞为T细胞。
44.根据权利要求37所述的免疫细胞，其中所述免疫细胞为B细胞。
45.根据权利要求37所述的免疫细胞，其中所述免疫细胞为人细胞。
46.根据权利要求37所述的免疫细胞，其中所述免疫细胞为灵长类细胞、啮齿动物细胞、猫细胞、狗细胞或马细胞。
47.根据权利要求1所述的多核苷酸，其中所述免疫细胞存活增强基因编码增强型信号传导受体(ESR)，其中所述ESR包含：
a.衍生自通常向免疫细胞传递抑制信号的受体的胞外结构域的序列
b.衍生自向免疫细胞传递刺激信号的受体的胞内结构域的序列
c.跨膜结构域
并且其中所述ESR不包含CD3ζ胞内结构域
48.根据权利要求47所述的多核苷酸，其中所述胞外结构域(a)衍生自选自以下的人蛋白质：TNFRSF3(LTRβ)、TNFRSF6(Fas)、TNFRSF8(CD30)、TNFRSF10A(DR4)、TNFRSF10B(DR5)、TNFRSF19(TROY)、TNFRSF21(DR6)和CTLA4。
49.根据权利要求47所述的多核苷酸，其中所述ESR包含与选自SEQ ID NO：322-340的序列具有至少90％同一性的序列。
50.根据权利要求47所述的多核苷酸，其中所述胞内结构域(b)衍生自选自以下的人蛋白质：TNFRSF4(OX40)、TNFRSF5(CD40)、TNFRSF7(CD27)、TNFRSF9(4-1BB)、TNFRSF11A(RANK)、TNFRSF13B(TACI)、TNFRSF13C(BAFF-R)、TNFRSF14(HVEM)、TNFRSF17(CD269)、TNFRSF18(GITR)、CD28、CD28H(TMIGD2)、诱导型T细胞共刺激因子(ICOS/CD278)、DNAX辅助分子1(DNAM-1/CD226)、信号转导淋巴细胞激活分子(SLAM/CD150)、T细胞免疫球蛋白和粘蛋白结构域(TIM-1/HAVcr-1)、干扰素受体α链(IFNAR1)、干扰素受体β链(IFNAR2)、白细胞介素2受体β亚基(IL2RB)、白细胞介素2受体γ亚基(IL2RG)、肿瘤坏死因子超家族14(TNFSF14/LIGHT)、自然杀伤细胞2族成员D(NKG2D/CD314)和CD40配体(CD40L)。
51.根据权利要求47所述的多核苷酸，其中所述ESR包含与选自SEQ ID NO：341-364的序列具有至少90％同一性的序列。
52.根据权利要求47所述的多核苷酸，其中所述ESR包含与选自SEQ ID NO：365-396的序列具有至少90％同一性的序列。
53.根据权利要求45所述的多核苷酸，其中所述ESR包含与选自SEQ ID NO：274-318的序列具有至少90％同一性的序列。
54.根据权利要求47所述的多核苷酸，其中所述多核苷酸还包含与异源启动子可操作地连接的编码凋亡抑制因子的片段。
55.一种免疫细胞，其基因组包含权利要求47所述的多核苷酸。
56.根据权利要求55所述的免疫细胞，其中所述免疫细胞基因组还包含与异源启动子可操作地连接的编码凋亡抑制因子的片段。
57.一种产生修饰的免疫细胞的方法，所述方法包括
a.向所述免疫细胞中导入权利要求47所述的多核苷酸。
b.将与异源启动子可操作地连接的编码凋亡抑制因子的多核苷酸导入所述免疫细胞中。
58.根据权利要求57所述的方法，其中将这两种多核苷酸同时导入所述免疫细胞中。
59.一种鉴定提高免疫细胞存活率的蛋白质的方法，包括
对来自癌性免疫细胞的编码蛋白质的核酸进行测序以鉴定编码具有突变的蛋白质的核酸；
用编码具有突变的所述蛋白质的核酸转化免疫细胞；以及确定所述免疫细胞是否具有提高的存活率。
CN202080027488.0A 2019-02-08 2020-02-07 基于转座子的免疫细胞的修饰 Pending CN114502731A (zh)

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