CN114432395A - 一种用于女性阴道清洁护理凝胶的制备方法 - Google Patents
一种用于女性阴道清洁护理凝胶的制备方法 Download PDFInfo
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Abstract
本发明属于护理用品技术领域,具体涉及一种用于女性阴道清洁护理凝胶的制备方法。一种用于女性阴道清洁护理凝胶,按重量百分比计由以下成分制备而成:白细胞提取物1‑5%、黄柏提取液2‑5%、丹参提取液2‑5%、地榆提取液2‑5%、苦参提取液2‑5%、当归提取液5‑10%、芦荟提取液5‑20%、β‑烟酰胺单核苷酸2‑5%、高分子透明质酸15‑20%,余量为纯化水。本发明的清洁护理凝胶由生物活性成分和中药草本精华制成,区别于传统女性阴道凝胶,用于清洁护理女性阴道时安全高效,可以修复阴道环境,排出因性生活、人流和经期等残留垃圾,充分发挥清理护理,抑菌滋养和紧致呵护的效果。
Description
技术领域
本发明属于护理用品技术领域,具体涉及一种用于女性阴道清洁护理凝胶的制备方法。
背景技术
阴道干涩是指阴道分泌物显著减少使阴道干燥,多由于年龄、压力、激素异常和炎症刺激等因素引起,为绝经期女性好发,伴有性交痛、外因瘙痒和外阴萎缩等现象。引起阴道干涩的原因比较多,主要包括内分泌失调、阴道炎和过度清洁,另外,饮食不均衡和干燥综合征等也可导致阴道干涩。
常规阴道护理凝胶大多为化学有机溶剂和抑菌剂混合制成,其虽然能在一定程度上解决阴道内壁的缺水及炎症,但一旦停用极易复发,治标不治本,而且长期或频繁使用还会对阴道粘膜造成极大伤害。
公开于该背景技术部分的信息仅仅旨在增加对本发明的总体背景的理解,而不应当被视为承认或以任何形式暗示该信息构成已为本领域一般技术人员所公知的现有技术。
发明内容
本发明的目的在于提供一种用于女性阴道清洁护理凝胶的制备方法,以解决背景技术中现有阴道护理凝胶存在的问题。
为实现上述目的,本发明提供了如下技术方案:
一种用于女性阴道清洁护理凝胶,按重量百分比计由以下成分制备而成:白细胞提取物1-5%、黄柏提取液2-5%、丹参提取液2-5%、地榆提取液2-5%、苦参提取液2-5%、当归提取液5-10%、芦荟提取液5-20%、β-烟酰胺单核苷酸2-5%、高分子透明质酸15-20%,余量为纯化水。
作为优选,所述白细胞提取物的制备方法包括以下步骤:
S1.取人外周血50-60mL,用淋巴细胞分离液密度梯度分离法分离出外周血单个核细胞;
S2.用ALys50N-0培养基洗涤所得外周血单个核细胞一次;
S3.用ALys50N-0培养基调整细胞浓度至1-2×106个/mL后置于T175培养瓶中,转移至培养箱中培养;
S4.2h后将T175培养瓶取出,取另一个T175培养瓶将悬浮细胞收集,加入1000IU/mL IFN-γ培养;
S5.24h后添加50ng/mL抗CD3单克隆抗体、1000IU/mL IL-2和100IU/mL IL-1α进行增殖培养;
S6.每2-3d添加一倍的ALys50N-0培养基,14d后收集细胞悬浮液进行冷冻干燥,获得白细胞提取物。
作为优选,所述女性阴道清洁护理凝胶制备方法为:按重量百分比计取各成分,震荡混匀即可。
与现有技术相比,本发明具有如下有益效果:
本发明凝胶制备原料中白细胞提取物富含40余种生物活性成分,可通过修复受损细胞,帮助肌肤产生胶原蛋白与弹力纤维,强化肌肤内部支撑结构,激活基底细胞,让细胞自我新生;同时可加速和促进肌肤微循环的建立,加速新陈代谢,促使肌肤自行排出毒素,从而全面提升肌肤状态,延缓皮肤衰老。
黄柏提取物主治功效:湿疹瘙痒,滋阴降火。用于阴虚火旺,盗汗骨蒸。
丹参提取物主治功效:抗感染,用于化脓性和外科感染,用于治疗宫颈糜烂。
地榆提取物主治功效:调节和改善机体免疫功能
苦参提取物主治功效:性寒,有清热燥湿和杀虫的功效,苦参浴能够清除下焦湿热,并且杀虫止痒,对皮肤瘙痒有很好的缓解作用,植物中草药能够平衡油脂分泌,疏通并收敛毛孔,清除皮肤内毒素杂质,丰富的本草营养,促进受损血管神经细胞的生长和修复,恢复皮下毛细血管细胞活力,肌肤重现紧致细滑
当归提取物主治功效:当归提取物是一种用于补血活血,调经止痛,润肠通便,血虚萎黄,眩晕心悸,月经不调,经闭痛经,虚寒腹痛,肠燥便秘,风湿痹痛,跌扑损伤,痈疽疮疡的植物提取物。
芦荟提取物可用作增稠剂、稳定剂、胶凝剂、粘结剂,用于一般食品。也用于化妆品等中:芦荟的蒽醌类化合物具有使皮肤收敛、柔软化、保湿、消炎、漂白的性能。还有解除硬化、角化、改善伤痕的作用,不仅能防止小皱纹、眼袋、皮肤松弛,还能保持皮肤湿润、娇嫩,同时,还可以治疗皮肤炎症。
烟酰胺单核苷酸在人体细胞能量生成中扮演重要角色,它参与细胞内NAD(烟酰胺腺嘌呤二核苷酸,细胞能量转化的重要辅酶)的合成。
透明质酸是一种多功能基质,广泛分布于人体各部位,其中皮肤也含有大量的透明质酸。人类皮肤成熟和老化过程也随着透明质酸的含量和新陈代谢而变化,它可以改善皮肤营养代谢,使皮肤柔嫩、光滑、去皱、增加弹性、防止衰老,在保湿的同时又是良好的透皮吸收促进剂,与其他营养成分配合使用,可以起到促进营养吸收的更理想效果。
本发明的清洁护理凝胶由以上生物活性成分和中药草本精华制成,区别于传统女性阴道凝胶,用于清洁护理女性阴道时安全高效,可以修复阴道环境,排出因性生活、人流和经期等残留垃圾,充分发挥清理护理,抑菌滋养和紧致呵护的效果。
具体实施方式
下面结合对本发明专利的技术方案进行清楚、完整的描述,显然,所描述的实施例是本发明的一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域所属的技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
在以下实施例中,黄柏提取液、丹参提取液、地榆提取液、苦参提取液、当归提取液、芦荟提取液和高分子透明质酸购置于广州美懿生物科技有限公司,其中高分子透明质酸分子量为1800000-2200000。β-烟酰胺单核苷酸购置于吉林百奥生物科技有限公司。
实施例1
白细胞提取物的制备方法,包括以下步骤:
S1.取人外周血50-60mL,用淋巴细胞分离液密度梯度分离法分离出外周血单个核细胞;
S2.用ALys50N-0培养基洗涤所得外周血单个核细胞一次,此时外周血单个核细胞的数量需为3-5×107个;
S3.用ALys50N-0培养基调整细胞浓度至1-2×106个/mL后置于T175培养瓶中,转移至培养箱中培养;
S4.2h后将T175培养瓶取出,取另一个T175培养瓶将悬浮细胞收集,加入1000IU/mL IFN-γ培养;
S5.24h后添加50ng/mL抗CD3单克隆抗体、1000IU/mL IL-2和100IU/mL IL-1α进行增殖培养;
S6.每2-3d添加一倍的ALys50N-0培养基,14d后收集细胞悬浮液进行冷冻干燥,获得白细胞提取物。
于第0d和第14d取样做细胞流式检测,结果见表1。
表1 细胞流式检测结果
CD3+CD56+(%) | CD3+CD8+(%) | 细胞成活率(%) | 细胞数量(个) | |
第0d | 11.2 | 19.6 | 98.1 | 7.5×107 |
第14d | 54.1 | 82.6 | 98.7 | 7.1×109 |
从表1可知,采用本发明的培养方法培养细胞14d后,CD3+CD56+、CD3+CD8+以及细胞数量均明显增加,同时细胞成活率也维持一个较高水平。
实施例2
一种用于女性阴道清洁护理凝胶,按重量百分比计由以下成分制备而成:白细胞提取物1%、黄柏提取液2%、丹参提取液3%、地榆提取液2%、苦参提取液3%、当归提取液6%、芦荟提取液8%、β-烟酰胺单核苷酸4%、高分子透明质酸17%,余量为纯化水。
实施例3
一种用于女性阴道清洁护理凝胶,按重量百分比计由以下成分制备而成:白细胞提取物2.5%、黄柏提取液4%、丹参提取液3%、地榆提取液3.5%、苦参提取液4%、当归提取液8%、芦荟提取液12%、β-烟酰胺单核苷酸3%、高分子透明质酸17%,余量为纯化水。
实施例4
一种用于女性阴道清洁护理凝胶,按重量百分比计由以下成分制备而成:白细胞提取物5%、黄柏提取液4%、丹参提取液3%、地榆提取液3%、苦参提取液5%、当归提取液9%、芦荟提取液18%、β-烟酰胺单核苷酸4%、高分子透明质酸18%,余量为纯化水。
上述实施例2-4中白细胞提取物由实施例1中的白细胞提取物的制备方法制备得到。
对比例1
与实施例3相比区别仅在于:缺少白细胞提取物。
使用者体验
200例试用者:年龄40-60岁,参照《临床诊疗指南-妇产科学分册》诊断标准,临床表现为:阴道分泌物增多;外阴瘙痒、灼热感,可伴有性交痛。将试用者随机分为2组:试验组和对照组,每组100人;其中试验组使用实施例3的凝胶,对照组使用对比例1的凝胶。
使用时,采取仰卧位,垫高臀部,将载有凝胶的凝胶器缓缓插入阴道内并退入阴道深处。使用完毕后保持卧位5-8min,避免凝胶过早流失。使用期间阴道内有正常排出物,需使用卫生护垫。于晚上睡觉前使用,隔天使用一次,连续使用4周,检测效果如表2。
表2 用于女性阴道清洁护理凝胶使用效果调查
从表2可以看出,试验组的使用者在阴道分泌物、瘙痒症状、同房紧致感和干涩感等方面均明显优于对照组,说明添加本发明的白细胞提取物可以有效提高凝胶的使用效果。
综上所述,本发明的凝胶可以修复阴道环境,排出因性生活、人流和经期等残留垃圾,充分发挥清理护理,抑菌滋养和紧致呵护的效果。
前述对本发明的具体示例性实施方案的描述是为了说明和例证的目的。这些描述并非想将本发明限定为所公开的精确形式,并且很显然,根据上述教导,可以进行很多改变和变化。对示例性实施例进行选择和描述的目的在于解释本发明的特定原理及其实际应用,从而使得本领域的技术人员能够实现并利用本发明的各种不同的示例性实施方案以及各种不同的选择和改变。本发明的范围意在由权利要求书及其等同形式所限定。
Claims (3)
1.一种用于女性阴道清洁护理凝胶,其特征在于,按重量百分比计由以下成分制备而成:白细胞提取物1-5%、黄柏提取液2-5%、丹参提取液2-5%、地榆提取液2-5%、苦参提取液2-5%、当归提取液5-10%、芦荟提取液5-20%、β-烟酰胺单核苷酸2-5%、高分子透明质酸15-20%,余量为纯化水。
2.根据权利要求1所述的用于女性阴道清洁护理凝胶,其特征在于,所述白细胞提取物的制备方法包括以下步骤:
S1.取人外周血50-60mL,用淋巴细胞分离液密度梯度分离法分离出外周血单个核细胞;
S2.用ALys50N-0培养基洗涤所得外周血单个核细胞一次;
S3.用ALys50N-0培养基调整细胞浓度至1-2×106个/mL后置于T175培养瓶中,转移至培养箱中培养;
S4.2h后将T175培养瓶取出,取另一个T175培养瓶将悬浮细胞收集,加入1000IU/mLIFN-γ培养;
S5.24h后添加50ng/mL抗CD3单克隆抗体、1000IU/mL IL-2和100IU/mL IL-1α进行增殖培养;
S6.每2-3d添加一倍的ALys50N-0培养基,14d后收集细胞悬浮液进行冷冻干燥,获得白细胞提取物。
3.根据权利要求1所述的用于女性阴道清洁护理凝胶,其特征在于,其制备方法为:按重量百分比计取各成分,震荡混匀,即得所述女性阴道清洁护理凝胶。
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