CN114402736A - Method for industrially and rapidly growing seedlings of red paeony seeds - Google Patents

Method for industrially and rapidly growing seedlings of red paeony seeds Download PDF

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CN114402736A
CN114402736A CN202210042983.XA CN202210042983A CN114402736A CN 114402736 A CN114402736 A CN 114402736A CN 202210042983 A CN202210042983 A CN 202210042983A CN 114402736 A CN114402736 A CN 114402736A
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seeds
temperature
treatment
seedling
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CN114402736B (en
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王俊杰
张晓明
赵彦
花梅
穆嬴通
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Inner Mongolia Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/60—Flowers; Ornamental plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to a method for industrially and rapidly culturing red paeony root seeds, which comprises the following steps: cup-filling sowing, seed warm-temperature treatment, seed low-temperature treatment, gibberellin treatment and seedling management; the warm-up lamination treatment temperature is 15-25 ℃, and the treatment time is 45 days; the low-temperature lamination treatment temperature is 2-6 ℃, and the treatment time is 30 days; gibberellin treatment includes: transplanting the seedling pot with the red peony seeds which is processed at low temperature into 15-20 ℃, and irrigating agricultural gibberellin every 7-8 days, wherein the concentration of the gibberellin is 500-1100mg/L, and the irrigation amount is 20 ml. The red peony seed industrialized rapid seedling raising method provided by the invention can obviously shorten the red peony seedling raising period, so that the red peony seedling raising time is short, the method is not limited by seasons, the seedling raising land is saved, and a technical support is provided for the practical industrialized rapid production and application of red peony seedling raising.

Description

Method for industrially and rapidly growing seedlings of red paeony seeds
Technical Field
The invention belongs to the technical field of red paeony root planting, and particularly relates to an industrialized rapid seedling raising method for red paeony root seeds
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
The propagation modes of the red paeony root (Paeoniae Radix Rubra) are divided into vegetative propagation and seed propagation. The vegetative propagation is mainly root-divided propagation, but the plants can be divided once in 3-5 years, and although the excellent characters of the female parent can be maintained, the propagation coefficient is low. The seed propagation can obtain more variation types, and has the advantages of high propagation coefficient and the like. However, the red peony seeds have the characteristic of double dormancy of upper and lower embryonic axes, 7 to 8 months are needed from sowing to seedling emergence, particularly, the seedling emergence can be realized after overwintering due to the alternation of high temperature, low temperature and high temperature in the processes of germination and seedling establishment, namely, the red peony seedling emergence needs 3 stages, namely, the dormancy of the lower embryonic axis is released at high temperature, the dormancy of the upper embryonic axis is released at low temperature, and the seedling emergence is realized at high temperature. Leading seeds to fail to emerge in the current year in the planting process of red peony root. Great troubles are caused to the seedling raising practice of the red paeony root seeds. At present, a complete and unified technical process is not formed in a field quick seedling emergence mode of red paeony seeds in the same year. However, a complete set of red peony seed industrialized rapid seedling raising method is not formed at present.
Disclosure of Invention
The invention aims to provide a method for industrially and rapidly growing seedlings of red paeony seeds, which aims to solve the problems of long time, seasonal limitation, high land growth and the like in the conventional red paeony seedling growing process.
The invention solves the problems through the following technical scheme:
a method for industrially and rapidly growing seedlings of red paeony seeds comprises the steps of cup-filling sowing, warm-temperature seed treatment, low-temperature seed treatment, gibberellin treatment and seedling management.
The cup-filling seeding comprises: and selecting a degradable seedling pot, wherein the culture medium is a mixture of sandy soil and cultivated soil.
Further, a degradable seedling pot with the upper opening diameter of 10cm in width and the height of 15cm is selected.
Furthermore, 2-10 seeds are sowed in each seedling pot.
Further, sand: the mass ratio of the cultivated soil is 1: 1.
the red seed warm-temperature treatment comprises the following steps: the warm-up lamination treatment temperature is 15-25 ℃, and the treatment time is 45 days;
preferably, the warm lamination treatment temperature is 20 ℃.
The low-temperature treatment of the red peony seeds comprises the following steps: the low-temperature lamination treatment temperature is 2-6 deg.C, and the treatment time is 30 days. Preferably, the low-temperature lamination treatment temperature is 4 ℃.
The gibberellin treatment includes: transplanting seedling pots with red peony roots, which are treated at low temperature, into 15-20 ℃, irrigating with agricultural gibberellin for 2-3 times, preferably 3 times, every 7-8 days; the concentration of gibberellin is 500-1100mg/L, and the irrigation amount is 20 ml. Preferably, the gibberellin concentration is 800mg/L.
The seedling management comprises the following steps: and (5) managing the culture temperature and water and fertilizer in the seedling stage after seedling emergence.
Further, when the true leaves are completely unfolded and cultured at 20-25 ℃, the environment humidity is not too high, and watering is carried out in time according to the water content of the matrix; after 10 days of complete leaf expansion, 50ml contained 0.52g/L P2O5、0.34g/L K2O, 0.002g/L Zn, 0.0005g/L Mn, 0.0005g/L B and 0.1g/L alginic acid.
According to the invention, the double dormancy of upper and lower embryonic axes of the red paeony seeds is released through the warm-temperature treatment and the low-temperature treatment of the seeds, so that the sprouting speed of the red paeony seeds is promoted. According to the method, the red peony seeds are treated by regulating and controlling proper temperature, and the rooting rate and the emergence rate of the red peony seeds are obviously improved by the cooperation of warm temperature and low temperature. The subsequent gibberellin treatment can promote the rooting rate and emergence rate of the seedlings on the basis of temperature treatment.
The invention has the beneficial effects that: the red peony seed industrialized rapid seedling raising method provided by the invention can obviously shorten the red peony seedling raising period, so that the red peony seedling raising time is short, the method is not limited by seasons, the seedling raising land is saved, and a technical support is provided for the practical industrialized rapid production and application of red peony seedling raising.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a flow chart of an implementation of the present invention.
FIG. 2 is a photograph of a seedling of radix Paeoniae Rubra as provided by an embodiment of the present invention.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The application of the principles of the present invention will now be described in detail.
The method for industrially and rapidly growing the seedlings of the red paeony seeds comprises the steps of cup-filling sowing, warm-temperature seed treatment, low-temperature seed treatment, gibberellin treatment and seedling management.
The selection of the seedling cup and the culture medium in the embodiment of the invention comprises the following steps:
and (4) selecting seeds, namely selecting full seeds from the seeds.
Selecting seedling pots, wherein the diameter of an upper opening of each seedling pot is 10cm in width and 15cm in height
Selection of culture medium, sand: the cultivated soil is mixed 1: 1.
And (4) sowing, wherein 2-10 seeds are sowed in each seedling pot.
The warm-temperature treatment method for the seeds comprises the following steps:
the temperature is chosen to be between 15 and 25 ℃ and preferably 20 ℃.
The low-temperature seed treatment method provided by the embodiment of the invention comprises the following steps:
the temperature is chosen to be between 2 and 6 ℃ and preferably 4 ℃.
The gibberellin storage treatment method provided by the embodiment of the invention comprises the following steps:
selection of culture temperature: transplanting the red peony seeds into a seedling pot at 15-20 ℃, and culturing in a greenhouse.
Selection of gibberellin concentration: the gibberellin concentration is 500-1100mg/L, preferably 800mg/L. 20ml of the mixture is irrigated every 7 to 8 days for 3 times.
The seedling management of the embodiment of the invention comprises the following steps:
selection of culture temperature: culturing in a greenhouse at 20-25 deg.C after the true leaves are completely unfolded.
Selecting water and fertilizer: watering in time according to the water content of the matrix; after 10 days of complete leaf expansion, 50ml contained 0.52g/L P2O5、0.34g/L K2O, 0.002g/L Zn, 0.0005g/L Mn, 0.0005g/L B and 0.1g/L alginic acid.
The application principle of the present invention will be further described with reference to experiments.
1, the experiment takes red paeony root seeds as materials, and realizes the quick seedling emergence of the red paeony root seeds in the current year by methods of warm-temperature treatment, low-temperature treatment, gibberellin irrigation, seedling stage management and the like.
2. Materials and methods
2.1 materials
The material is Hulunbel wild red peony seed.
2.2 methods
Warm sand storage treatment of red peony seeds: performing warm-warm stratification treatment on the red paeony root seeds for 45 days. After warm sand storage treatment, the red peony seeds are subjected to low-temperature stratification for 30 days. Transferring the red peony seeds after low-temperature treatment to 15-20 ℃. Agricultural gibberellin irrigation is carried out every 7-8 days. And (5) irrigating for three times.
2.2.1 Warm Sand storage treatment of radix Paeoniae Rubra seeds
(1) Temperature single factor test
Warm sand storage treatment of red peony seeds: the red peony seeds are subjected to warm stratification, ten seeds are placed in each seedling cup, and ten treatments are repeated. The treatment temperature is 5 deg.C, 10 deg.C, 15 deg.C, 20 deg.C, 25 deg.C, and the treatment time is 45 days. After 45 days, the ratio of rooting rate and root length of 4-5cm is observed.
2.2.1 Low temperature Sand storage treatment and gibberellin concentration treatment of radix Paeoniae Rubra seeds
(1) Temperature and gibberellin concentration two-factor test
And (3) carrying out low-temperature sand storage treatment on the red peony seeds: performing low-temperature stratification treatment on the radix Paeoniae Rubra seeds at 2 deg.C, 4 deg.C and 6 deg.C for 30 days. After 30 days, the cells are transferred to 15 to 20 ℃ for gibberellin irrigation, and the concentration is 500mg/L, 800mg/L and 1100 mg/L. Agricultural gibberellin is irrigated for three times every 7 to 8 days. Ten seeds were placed per seedling cup and ten replicates per treatment were performed.
2.3 data statistics and analysis
The rooting rate (%) is the number of rooted seeds/total number of seeds × 100%
Emergence rate (%) -. number of seeds emerging/total number of seeds X100%
The proportion of the seeds with the root length of 4-5cm (%) -the total number of the seeds with the root length of 4-5 cm/the rooted seeds is multiplied by 100%
3. Results and analysis
3.1 Effect of temperature on root growth of radix Paeoniae Rubra seeds
The best rooting treatment is 20 ℃, the rooting rate is 82.45 percent, the proportion of 4-5cm root length is 70 percent, and the rooting effect of different treatments is shown in table 1
Figure BDA0003471017620000051
Effect of temperature and gibberellin concentration on the emergence of Red peony seeds
The best treatment was carried out at a temperature of 800mg/L (Table 2)
TABLE 2 Effect of different treatments
Figure BDA0003471017620000061
Conclusion, from tables 1 and 2, it is known that red peony seeds were subjected to warm-temperature stratification treatment at a treatment temperature of 20 ℃ for 45 days (2) seed low-temperature sand storage treatment at a treatment temperature of 4 ℃ for 30 days (3) agricultural gibberellin treatment: transferring the radix Paeoniae Rubra seeds treated at low temperature into 15-20 deg.C environment. 20ml of agricultural gibberellin is irrigated every 7 to 8 days. And (5) irrigating for three times. The gibberellin concentration is 800mg/L.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (10)

1. A method for industrially and rapidly growing seedlings of red paeony seeds is characterized by comprising the following steps:
(1) cup filling and seeding: selecting a degradable seedling pot, wherein a culture medium is a mixture of sandy soil and cultivated soil;
(2) seed warm-up treatment: the warm-up lamination treatment temperature is 15-25 ℃, and the treatment time is 45 days;
(3) seed low-temperature treatment: the low-temperature lamination treatment temperature is 2-6 ℃, and the treatment time is 30 days;
(4) gibberellin treatment: transplanting seedling pot with radix Paeoniae Rubra seed treated at low temperature into 15-20 deg.C, irrigating with gibberellin every 7-8 days for 2-3 times; the concentration of gibberellin is 500-1100mg/L, and the irrigation amount is 20 mL;
(5) seedling stage management: and (5) managing the culture temperature and water and fertilizer in the seedling stage after seedling emergence.
2. The method for industrially and rapidly growing seedlings of red paeony seeds as claimed in claim 1, wherein degradable seedling pots with the upper opening diameter of 10cm in width and the height of 15cm are selected for sowing.
3. The method for the industrialized rapid seedling culture of red paeony seeds as claimed in claim 1, wherein 2 to 10 seeds are sown in each seedling pot.
4. The method for industrially and rapidly growing seedlings of red paeony seeds as claimed in claim 1, wherein in the culture medium, the ratio of sand: the mass ratio of the cultivated soil is 1: 1.
5. the method for industrially and rapidly culturing the seedlings of the red paeony seeds as claimed in claim 1, wherein the temperature for warm-temperature stratification is 20 ℃.
6. The method for industrially and rapidly culturing the seedlings of the red paeony seeds as claimed in claim 1, wherein the low-temperature stratification treatment temperature is 4 ℃.
7. The method for industrially and rapidly growing seedlings of red paeony seeds as claimed in claim 1, wherein the gibberellin is treated with water for 3 times at a concentration of 800mg/L.
8. The method for industrially and rapidly growing seedlings of red paeony seeds as claimed in claim 1, wherein the seedling management comprises the following steps: culturing at 20-25 deg.C after true leaf is completely unfolded.
9. The method for industrially and rapidly growing seedlings of red paeony seeds as claimed in claim 1, wherein the seedling management comprises the following steps: watering in time according to the water content of the matrix; after the leaves are completely unfolded for 10 days, the leaves are irrigated once with 50mL of nutrient solution.
10. The method for industrially and rapidly culturing the seedlings of the red paeony seeds as claimed in claim 9, wherein the nutrient solution is: containing 0.52g/L P2O5、0.34g/L K2O, 0.002g/L Zn, 0.0005g/L Mn, 0.0005g/L B and 0.1g/L alginic acid.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105123250A (en) * 2015-09-28 2015-12-09 中国科学院植物研究所 Method for promoting red paeony root seed sprouting and application of method in red paeony root propagation
CN106561110A (en) * 2016-11-03 2017-04-19 张晓峰 Germination accelerating method of peony seeds

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105123250A (en) * 2015-09-28 2015-12-09 中国科学院植物研究所 Method for promoting red paeony root seed sprouting and application of method in red paeony root propagation
CN106561110A (en) * 2016-11-03 2017-04-19 张晓峰 Germination accelerating method of peony seeds

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张捷 等: "芍药播种繁殖中种子破眠技术的研究进展", 《分子植物育种》 *
袁燕波 等: "外源GA3与低温处理对芍药种子萌发和幼苗生长的影响", 《安徽农业大学学报》 *

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