CN111670808A - Cultivation method of purple bud dancing flower ginger seedlings - Google Patents
Cultivation method of purple bud dancing flower ginger seedlings Download PDFInfo
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- CN111670808A CN111670808A CN202010647452.4A CN202010647452A CN111670808A CN 111670808 A CN111670808 A CN 111670808A CN 202010647452 A CN202010647452 A CN 202010647452A CN 111670808 A CN111670808 A CN 111670808A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention belongs to the technical field of plant cultivation, and discloses a cultivation method of Zibud dancing flower ginger seedlings, which comprises the steps of carrying out greenhouse cultivation on Zibud dancing flower ginger plants, cutting off new buds from the plants, stripping off external leaves, leaving a small amount of heart leaves, sterilizing, and carrying out inoculation cultivation; growing the buds into seedlings of 1.0-1.5 cm; inoculating the plantlet to a proliferation culture medium for culture; cutting the adventitious bud with the height less than 3.0cm and inoculating the adventitious bud with the height less than 3.0cm to a proliferation culture medium for growth after the plantlet grows into a group adventitious bud with the height of 2.0-5.0 cm; cutting adventitious buds with height more than 3.0cm, inoculating into strong seedling rooting culture medium, culturing until the adventitious buds grow to required specification (more than 3 roots and more than 3.0cm root length), hardening seedlings, and moving out of culture flask for planting. The method is not influenced by factors such as seasons and the like, the method for efficiently and quickly providing the high-quality seedlings of the Zibud dancing Hedychium spicatum can accelerate the planting and popularization of the Zibud dancing Hedychium spicatum and promote the industrial development of the Zibud dancing Hedychium spicatum.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a cultivation method of Zibud dancing flower ginger seedlings.
Background
At present, the Zibao dancing flower ginger is a flower with roots and stems generating heat for many years, has peculiar inflorescence shape, bright color, proper growth temperature of 25-35 ℃, poor cold resistance, plant growth stopping at the temperature of below 10 ℃, difficulty in overwintering in the north of Jiangzhe, usually adopts a vegetative propagation method of rhizome cutting or division for propagation in production, has low propagation coefficient, and seriously hinders the industrialized production of the Zibao dancing flower ginger.
Therefore, the method for efficiently cultivating the Zibud dancing flower ginger seedlings can produce enough high-quality seedlings and can powerfully promote the industrial development of the Zibud dancing flower ginger.
Through the analysis, the problems and the defects of the asexual propagation method adopting rhizome cutting or splitting for seedling propagation of Zibud dancing ginger are as follows:
(1) in order to meet the requirement of seedlings required in production, a large amount of mother seeds are consumed;
(2) the propagation coefficient is low, the annual propagation coefficient is about 10 generally, and a large number of high-quality seedlings with the same genetic background and consistent specification cannot be produced in a short time;
(3) the high-quality seedlings can not be provided for a long time all year round due to the limitation of seasons, regions and the like;
(4) is easy to carry and spread diseases and pests.
The difficulties of the above problems and defects by using the vegetative propagation method of rhizome cutting or division are as follows:
(1) a large number of mother seeds are required.
(2) The propagation coefficient is low, and the annual propagation coefficient is about 10 generally.
(3) The annual long-term supply of high-quality seedlings cannot be realized.
(4) Is easy to carry and spread diseases and pests.
The significance of solving the above problems and drawbacks: the method uses a small amount of mother seeds, occupies small space, has high propagation coefficient, is not limited by seasons, regions and the like, can produce a large amount of high-quality Zibud dancing flower ginger seedlings with the same genetic background, consistent specifications and no plant diseases and insect pests in a short time, and powerfully promotes the industrial development of the Zibud dancing flower ginger.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for cultivating Zibud dancing flower ginger seedlings.
The invention is realized in such a way that a method for cultivating Zibud dancing flower ginger seedlings comprises the following steps:
1) putting healthy and strong Zibud dancing galangal plants without disease spots into an intelligent greenhouse for culturing until the base of the Zibud dancing galangal plants germinates to give new buds with the height of 2.0-3.0 cm; the culture conditions were: culturing under illumination at natural temperature;
2) cutting off the buds from the plant, stripping off external leaves, leaving a small amount of heart leaves, and inoculating the buds to an induction culture medium for culture after conventional disinfection; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the illumination and the dark culture are alternately carried out;
3) when the buds are induced to start growing into small seedlings with the height of about 1.0-1.5 cm;
4) inoculating the plantlet to a proliferation culture medium for culture; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the illumination and the dark culture are alternately carried out;
5) cutting and inoculating adventitious buds with the height of less than 3.0cm into a proliferation culture medium for culture when the plantlets grow into adventitious buds with the height of 2.0-5.0 cm; cutting adventitious buds with height more than 3.0cm, inoculating into strong seedling rooting culture medium, culturing until the adventitious buds grow to required specification (more than 3 roots and more than 3.0cm root length), hardening seedlings, and moving out of a culture bottle for planting; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
6) And repeating the step 5) when the plantlets in the multiplication culture medium in the step 5) grow into adventitious buds of the family with the height of 2.0-5.0 cm.
Further, the induction medium in step 2) is: 1/2MS minimal medium, 3.0-5.0% sugar and 0.35-0.50% agar, and the pH value is 5.5-6.0.
The proliferation culture medium in the step 4) is as follows: MS basic culture medium, 2.0-3.0 mg/l 6-benzyladenine, 0.01-0.02 mg/l naphthylacetic acid, 3.0-5.0% sugar and 0.35-0.50% agar, wherein the pH value is 5.5-6.0.
The strong seedling rooting culture medium in the step 5) comprises the following components: 1/2MS minimal medium, 0.02-0.05 mg/l naphthylacetic acid, 3.0-5.0% sugar and 0.35-0.50% agar, and the pH value is 5.5-6.0.
By combining all the technical schemes, the invention has the advantages and positive effects that: uses a small amount of mother seeds, occupies small space, is not limited by seasons, regions and the like, and can produce a large amount of high-quality Zibao dancing flower ginger seedlings with the same genetic background, consistent specification and no plant diseases and insect pests in a short time.
The invention discloses a method for cultivating Zibud dancing flower ginger seedlings, and belongs to a tissue culture method for inducing and proliferating Zibud dancing flower ginger test-tube seedlings. According to the principle of cell totipotency in plant tissue culture, a large number of high-quality seedlings with the same genetic background and consistent growth vigor can be produced in a short time, and the high-quality seedlings can be provided for a long time all year round by depending on a laboratory. In the method, the strong Zibao dancing ginger plants are put into an intelligent greenhouse for culture and germination acceleration, so that a certain amount of aseptic seedlings can be obtained by induction, and the test-tube seedlings are obtained by proliferation, strong seedlings and rooting. By adopting the method, the propagation coefficient of the Zibud dancing flower ginger seedlings in one year is more than 100 ten thousand times, the field transplanting survival rate of the rooted seedlings reaches more than 90 percent, a large amount of mother seeds are consumed by a rhizome cutting or plant division propagation method, the annual propagation coefficient is low (about 10), the method is limited by seasons, regions and the like, high-quality seedlings cannot be provided for a long time in one year, and the method is easy to carry and spread plant diseases and insect pests. Therefore, the method for cultivating the purple bract dancing flower ginger seedlings is not influenced by factors such as seasons and the like, can efficiently and quickly provide high-quality purple bract dancing flower ginger seedlings, can accelerate the planting and popularization of the purple bract dancing flower ginger, and promotes the industrial development of the purple bract dancing flower ginger.
(3) Comparative technical or experimental results (see table below).
Comparison of the novel cultivation method with the rhizome cutting or plant division propagation method
| New cultivation method | Rhizome cutting or plant division propagation method | |
| Amount of mother seed | Small amount, even only one bud | A large number of |
| Annual reproduction coefficient (times) | More than 1000000 | About 10 |
| Limitation of season and region | Is free of | Is provided with |
| Plant diseases and insect pests | Is not portable | Is easy to carry |
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a method for cultivating Zibud dancing ginger seedlings according to an embodiment of the present invention.
FIG. 2(a) is a proliferation culture (prophase) provided in an example of the present invention; 2(b) is the ethnic adventitious bud provided by the embodiment of the invention; FIG. 2(c) is a proliferation culture (late phase) provided by an embodiment of the present invention; FIG. 2(d) is a drawing of strong seedling rooting culture provided by the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for cultivating Zibud dancing flower ginger seedlings, and the invention is described in detail with reference to the attached drawings.
As shown in figure 1, the method for cultivating Zibud dancing flower ginger seedlings provided by the invention comprises the following steps:
s101, putting healthy and strong Zibud dancing galangal plants without disease spots into an intelligent greenhouse for culturing until the base of the Zibud dancing galangal plants germinate to emit new buds with the height of 2.0-3.0 cm; the culture conditions were: and (5) culturing under illumination at natural temperature.
S102, cutting off the buds from the plants, stripping off external leaves, leaving a small amount of heart leaves, and inoculating the buds to an induction culture medium for culture after conventional disinfection; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
S103, after the bud is induced and started to grow into a seedling with the height of about 1.0-1.5 cm.
S104, inoculating the plantlets to a proliferation culture medium for culture; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
S105, cutting and inoculating adventitious buds with the height of less than 3.0cm into the enrichment medium of the step S104 for culture when the seedlings grow into adventitious buds with the height of 2.0-5.0 cm; cutting adventitious buds with height more than 3.0cm, inoculating into strong seedling rooting culture medium, culturing until the adventitious buds grow to required specification (more than 3 roots and more than 3.0cm root length), hardening seedlings, and moving out of a culture bottle for planting; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
S106, when the plantlets grow to form adventitious buds of the families with the height of 2.0-5.0 cm in the enrichment medium in the step S105, repeating the step S105.
As a preferred method of the invention, the induction medium in step 2) is: 1/2MS minimal medium, 3.0-5.0% sugar and 0.35-0.50% agar, and the pH value is 5.5-6.0.
The preparation method of the induction culture medium comprises the following specific steps: using 1/2MS minimal medium (namely the content of all substances in the solution is half of the MS minimal medium) as a base, respectively adding sugar and agar, uniformly mixing, and adjusting the pH to 5.5-6.0 by using 1.0mol/L KOH or 1.0mol/L HCl; 30-50 g of sugar and 3.5-5.0 g of agar are added into 1L of 1/2MS minimal medium.
As a preferred method of the invention, the propagation medium in step 4) is: MS basic culture medium, 2.0-3.0 mg/l 6-benzyladenine, 0.01-0.02 mg/l naphthylacetic acid, 3.0-5.0% sugar and 0.35-0.50% agar, wherein the pH value is 5.5-6.0.
The preparation method of the proliferation culture medium comprises the following specific steps: taking an MS basic culture medium as a base, respectively adding 6-benzyladenine, naphthylacetic acid, sugar and agar, uniformly mixing, and adjusting the pH to 5.5-6.0 by using 1.0mol/L KOH or 1.0mol/L HCl; 2.0-3.0 mg of 6-benzyladenine, 0.01-0.02 mg of naphthylacetic acid, 30-50 g of sugar and 3.5-5.0 g of agar are added into each 1L of MS minimal medium.
As a preferred method of the invention, the strong seedling rooting culture medium in the step 5) is as follows: 1/2MS minimal medium, 0.02-0.05 mg/l naphthylacetic acid, 3.0-5.0% sugar and 0.35-0.50% agar, and the pH value is 5.5-6.0.
The preparation method of the induction culture medium comprises the following specific steps: using 1/2MS minimal medium (namely the content of all substances in the solution is half of the MS minimal medium) as a base, respectively adding naphthylacetic acid, sugar and agar, uniformly mixing, and adjusting the pH to 5.5-6.0 by using 1.0mol/L KOH or 1.0mol/L HCl; 0.02-0.05 mg of naphthylacetic acid, 30-50 g of sugar and 3.5-5.0 g of agar are added into each 1L of 1/2MS minimal medium.
Those of ordinary skill in the art to which the invention pertains may implement additional steps, and the invention of fig. 1 is provided as a specific example only.
The present invention will be further described with reference to specific experimental effects.
The growth culture (prophase) was carried out as shown in FIG. 2 (a). FIG. 2(b) shows adventitious buds. FIG. 2(c) proliferation culture (late stage). FIG. 2(d) is a drawing of strong seedling rooting culture.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. A cultivation method of Zibud dancing flower ginger seedlings is characterized by comprising the following steps:
step one, carrying out greenhouse culture on healthy and strong Zibud dancing galangal plants without disease spots until a new bud with the height of 2.0-3.0 cm is sprouted from the bases of the Zibud dancing galangal plants;
step two, cutting off the buds from the plants, stripping off external leaves, leaving a small amount of heart leaves, sterilizing, and inoculating to an induction culture medium for culture;
step three, after the bud is induced to start growing into a seedling with the height of 1.0-1.5 cm;
step four, inoculating the plantlets to a proliferation culture medium for culture;
step five, cutting and inoculating the adventitious buds with the height of less than 3.0cm into the multiplication culture medium in the step four for culture after the plantlets grow into adventitious buds with the height of 2.0-5.0 cm; cutting adventitious buds with the height of more than 3.0cm, inoculating the adventitious buds into a strong seedling rooting culture medium for culture until the adventitious buds grow to the required specification, hardening seedlings, moving out of a culture bottle for planting;
step six, repeating the step five when the adventitious buds in the multiplication culture medium in the step five grow into the group adventitious buds with the height of 2.0-5.0 cm.
2. The method for cultivating Zibud dancing Zingiber officinale seedlings according to claim 1, wherein the first cultivation condition is: and (5) culturing under illumination at natural temperature.
3. The method for cultivating Zibud dancing Zingiber officinale seedlings according to claim 1, wherein the culture conditions in the second step are as follows: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
4. The method for cultivating Zibud dancing Zingiber officinale seedlings according to claim 1, wherein the induction medium in the second step is: 1/2MS minimal medium, 3.0-5.0% sugar and 0.35-0.50% agar, and the pH value is 5.5-6.0.
5. As in claimThe method for cultivating the Zibud dancing ginger seedlings, which is characterized in that the culture conditions in the fourth step are as follows: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
6. The method for cultivating Zibud dancing Zingiber officinale seedlings according to claim 1, wherein the multiplication medium of the fourth step is: MS basic culture medium, 2.0-3.0 mg/l 6-benzyladenine, 0.01-0.02 mg/l naphthylacetic acid, 3.0-5.0% sugar and 0.35-0.50% agar, wherein the pH value is 5.5-6.0.
7. The method for cultivating Zibud dancing Zingiber officinale seedlings according to claim 1, wherein the step five adventitious buds are cut and inoculated into a strong seedling rooting culture medium for cultivation; the culture conditions were: 12 hours of illumination with the illumination intensity of 20-30 mu mol m-2.s-1The temperature is 24-26 ℃; dark culture is carried out for 12 hours at the temperature of 24-26 ℃; the above-mentioned light irradiation and dark culture were alternately performed.
8. The method for cultivating Zibud dancing Zingiber officinale seedlings according to claim 1, wherein the strong seedling rooting medium in the fifth step is: 1/2MS minimal medium, 0.02-0.05 mg/l naphthylacetic acid, 3.0-5.0% sugar and 0.35-0.50% agar, and the pH value is 5.5-6.0.
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| CN112616675A (en) * | 2021-01-11 | 2021-04-09 | 中国科学院华南植物园 | Tissue culture and rapid propagation method for Zingiber dance |
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