CN105123250A - Method for promoting red paeony root seed sprouting and application of method in red paeony root propagation - Google Patents
Method for promoting red paeony root seed sprouting and application of method in red paeony root propagation Download PDFInfo
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- CN105123250A CN105123250A CN201510629329.9A CN201510629329A CN105123250A CN 105123250 A CN105123250 A CN 105123250A CN 201510629329 A CN201510629329 A CN 201510629329A CN 105123250 A CN105123250 A CN 105123250A
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- seed
- seeds
- paeony root
- red paeony
- radix paeoniae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a method for promoting red paeony root seed sprouting and application of the method in red paeony root propagation. The method comprises the following steps: (1) soaking red paeony root seeds in a gibberellin solution; (2) after the step (1) is finished, taking the soaked seeds, and burying the soaked seeds with sand; (3) after the step (2) is finished, taking rooted seeds, sowing the rooted seeds, and culturing the rooted seeds till the rooted seeds sprout, wherein the gibberellin solution is sprayed on the rooted seeds during the culture process. The method has the advantages that the dormancy of the red paeony root seeds is broken down effectively, so as to promote seed sprouting; the red paeony root seeds can sprout fast, the sprouting time is greatly shortened, and the sprouting rate is improved; the operation is simple, the cost is low, and the method can be widely applied to field production. The method is highly remarkable in promoting seedling raising and propagation of red paeony root, thereby having a huge economic benefit when being applied to the red paeony root industry.
Description
Technical field
The present invention relates to a kind of promote the method for radix paeoniae rubrathe seed sprouting and the radix paeoniae rubrathe expand numerous in application.
Background technology
Chinese herbaceous peony is China's tradition famous flower, and cultivation history is long, and spend gorgeous greatly, have ornamental value, its root can make the radix paeoniae rubrathe or the root of herbaceous peony is used as medicine.It is good that radix paeoniae rubrathe quality is produced in Duolun County, Inner Mongolia, and paeoniflorin content is high, is regarded as genunie medicinal materials.Along with excavating of people, Duolun Chishao can not be met the need of market, is badly in need of amount reproduction.
In production, Chinese herbaceous peony breeding is based on division propagation, and once, the cycle is long in 3-5 plant division, and reproduction coefficient is low, is difficult to the demand meeting industrialization production & marketing.
Seminal propagation coefficient is high, and gained plant patience is strong, and can be mass-produced, but radix paeoniae rubrathe seed has the characteristic of dual dormancy, seminal propagation needs breaking dormancy, and the cycle used is longer, and germination rate is lower.
In prior art, radix paeoniae rubrathe seed seedling-raising is mainly by refrigeration.Generally sow in autumn in production, then the refrigeration through a winter emerge, and emergence rate is about 10%.Normally the seed of taking root refrigeration is promoted to emerge with breaking dormancy for 1-2 month in document.
Therefore, the method for seeking the quick breaking dormancy of a kind of energy, promoting seed sprouting, emerging is necessary.
Summary of the invention
The object of this invention is to provide a kind of promote the method for radix paeoniae rubrathe seed sprouting and the radix paeoniae rubrathe expand numerous in application.
The invention provides a kind of method promoting radix paeoniae rubrathe seed sprouting, comprise the steps:
(1) get RADIX PAEONIAE RUBRA seed, soak seed in Gibberellins solution;
(2), after completing steps (1), seed is got and sand buries;
(3) after completing steps (2), get seed of taking root, sow and be cultured to and emerge, in incubation, spray Gibberellins solution.
In described step (1), the gibberellin concentration in Gibberellins solution is 0.5mM.In described step (1), described seed soaking is room temperature seed soaking.In described step (1), the time of described seed soaking is 2 days.
In described step (2), the time that described sand buries is 50-100 days, is preferably 60 days.In described step (2), described sand buries as burying in vermiculite.In described step (2), described " burying in vermiculite " is " in vermiculite, room temperature is buried ".In described step (2), the water content of described vermiculite is 8% ± 2%.
In described step (3), the gibberellin concentration in Gibberellins solution is 1mM.In described step (3), the culture matrix of described cultivation is vermiculite.In described step (3), the temperature of described cultivation is 18-30 DEG C.In described step (3), described seed of having taken root is take root and the long seed for more than 3cm of root.In described step (3), after planting seed is apart from 2.5 centimetres ± 0.5 centimetre, vermiculite surface.In described step (3), within after planting the 1 to 3 day, spray Gibberellins solution.In described step (3), described in the dosage that sprays be as the criterion to realize " ensure that topsoil is moistening, can seed be touched in infitration process ".In described step (3), planting seed is 13cm in diameter, is highly the flowerpot of 10cm, within after planting the 1 to 3 day, sprays Gibberellins solution, and every day is each sprays flowerpot 5ml.
The solvent of arbitrary described Gibberellins solution is 0.5mM calcium chloride water above.
The present invention also protects the application of above arbitrary described method in radix paeoniae rubrathe expansion is numerous
Arbitrary described radix paeoniae rubrathe can be wild RADIX PAEONIAE RUBRA above.Arbitrary described radix paeoniae rubrathe specifically can be the wild RADIX PAEONIAE RUBRA that Duolun County, Inner Mongolia gathers within the border above.Arbitrary described Chinese herbaceous peony is red above specifically can be the wild RADIX PAEONIAE RUBRA in Duolun.
Method tool provided by the present invention has the following advantages: can effectively abolish radix paeoniae rubrathe seed dormancy, promotes seed germination; Can promote that radix paeoniae rubrathe seed is emerged fast, effectively shorten seedling-growing time, improve emergence rate; Simple to operate, with low cost, can Production of Large Fields be widely used in.The present invention is numerous with expansion for the nursery of the radix paeoniae rubrathe, and its effect is and significantly, is extended to radix paeoniae rubrathe industry and can produce very large economic benefit.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment for three times, results averaged.Flowerpot diameter is 13cm, highly for 10cm.The preparation method of Gibberellins solution: gibberellin is dissolved in 0.5mM calcium chloride water.Room temperature refers to greenhouse (night, minimum temperature was 18 DEG C, and daytime, maximum temperature was 30 DEG C).Wild RADIX PAEONIAE RUBRA seed: pick up from Cai Mu mountain area, Duolun County, Inner Mongolia.
Embodiment 1 (embodiment under preferred parameter),
1, get radix paeoniae rubrathe seed, soak seed 2 days by 0.5mM Gibberellins solution room temperature, seed is all not mouldy.
2, after completing steps 1, get seed, in moistening vermiculite, (water content is 8% ± 2%) room temperature buries 60 days.
3, after completing steps 2, get and take root and the long seed for more than 3cm of root, be seeded in flowerpot and (vermiculite be housed, as culture matrix in flowerpot; 100 seeds are sowed in each flowerpot; Seed is apart from 2.5 centimetres ± 0.5 centimetre, vermiculite surface), be then divided into three groups, often organize 3 basins, respectively process following (using the initial time of packet transaction as 0 moment, count number of days from 0 moment, every 24 hours is 1 day):
First group: incubated at room temperature;
Second group (prior art): first cultivate 40 days for 4 DEG C, then incubated at room temperature;
3rd group (technology of the present invention): the 1st day to the 3rd day every day, every basin sprayed 5ml1mM Gibberellins solution (at every turn spraying rear overlay film to reduce evaporation), incubated at room temperature.
Observe every day and add up emergence rate, exposing vermiculite surface with young shoot and be considered as emerging.
Add up the seedling-growing time first of each group of process, the emergence rate of time when emergence rate is 50%, the 100th day, the results are shown in Table 1 (mean values of three repeated tests).
Emergence rate under table 1 different disposal condition and emerging the time used
Do not emerge for first group, second group close with the emergence rate of the 3rd group, illustrates that gibberellin process and chilling treatment all can break the epicotylar dormancy of radix paeoniae rubrathe seed, promotes seed sprouting.The seedling-growing time of the 3rd group is significantly shorter than second group, illustrates that gibberellin process effectively can promote that radix paeoniae rubrathe seed is emerged fast, significantly shortens seedling-growing time.
Groping of embodiment 2, relevant parameter
One, seed soaking time
Get radix paeoniae rubrathe seed, to soak seed 2 days or 5 days by 1mM Gibberellins solution room temperature.
Carry out three repeated tests, 100 seeds are got in each process of each repeated test.
Seed after 2 days of soaking seed all does not go mouldy.Soak seed after 5 days, have the seed of 8% ± 1% there occurs and go mouldy.
Two, gibberellin concentration during seed soaking and bury the time when burying
1, get radix paeoniae rubrathe seed, soak seed 2 days by Gibberellins solution (gibberellin concentration is 0.001mM, 0.01mM, 0.1mM, 0.5mM, 1mM or 5mM, arranges and replaces the contrast CK of Gibberellins solution with the 0.5mM calcium chloride solution) room temperature of variable concentrations.Seed is all not mouldy.
2, after completing steps 1, get seed, in moistening vermiculite, (water content is 8% ± 2%) room temperature buries 100 days.50th day statistics Seed sprouting rate (breaking through seed coat with radicle to be considered as sprouting), 60th day statistics seed rooting rate (seed is taken root and root length is considered as taking root for more than 3cm), 85th day statistics seed rooting rate, the 100th day statistics seed rooting rate.The results are shown in Table 2 (get the mean value of three repeated tests, in each repeated test, each process is containing 100 seeds).
Table 2 Seed sprouting rate and seed rooting rate ("-" representative is not added up)
Gibberellin concentration when three, spraying
1, get radix paeoniae rubrathe seed, soak seed 2 days by 0.5mM Gibberellins solution room temperature, seed is all not mouldy.
2, after completing steps 1, get seed, in moistening vermiculite, (water content is 8% ± 2%) room temperature buries 60 days.
3, after completing steps 2, get and take root and the long seed for more than 3cm of root, be seeded in flowerpot and (vermiculite be housed, as culture matrix in flowerpot; 100 seeds are sowed in each flowerpot; Seed is apart from 2.5 centimetres ± 0.5 centimetre, vermiculite surface), be then divided into five groups, often organize 3 basins, respectively process following (using the initial time of packet transaction as 0 moment, count number of days from 0 moment, every 24 hours is 1 day):
First group: 1st day to the 3rd day every day, every basin sprayed 5ml0.5mM calcium chloride water (at every turn spraying rear overlay film to reduce evaporation), incubated at room temperature.
Second group: 1st day to the 3rd day every day, every basin sprayed 5ml0.01mM Gibberellins solution (at every turn spraying rear overlay film to reduce evaporation), incubated at room temperature.
3rd group: the 1st day to the 3rd day every day, every basin sprayed 5ml0.1mM Gibberellins solution (at every turn spraying rear overlay film to reduce evaporation), incubated at room temperature.
4th group: the 1st day to the 3rd day every day, every basin sprayed 5ml0.5mM Gibberellins solution (at every turn spraying rear overlay film to reduce evaporation), incubated at room temperature.
5th group: the 1st day to the 3rd day every day, every basin sprayed 5ml1mM Gibberellins solution (at every turn spraying rear overlay film to reduce evaporation), incubated at room temperature.
Add up the emergence rate of the seedling-growing time first of each group of process, the emergence rate of the 30th day, the emergence rate of the 50th day and the 100th day, the results are shown in Table 3 (mean values of three repeated tests).
Emergence rate under table 3 different disposal condition and emerging the time used
Claims (10)
1. promote a method for radix paeoniae rubrathe seed sprouting, comprise the steps:
(1) get RADIX PAEONIAE RUBRA seed, soak seed in Gibberellins solution;
(2), after completing steps (1), seed is got and sand buries;
(3) after completing steps (2), get seed of taking root, sow and be cultured to and emerge, in incubation, spray Gibberellins solution.
2. the method for claim 1, is characterized in that: in described step (1), and the gibberellin concentration in Gibberellins solution is 0.5mM.
3. as claim 1 or as described in method, it is characterized in that: in described step (1), the time of described seed soaking is 2 days.
4. as the method as described in arbitrary in claims 1 to 3, it is characterized in that: in described step (2), the time that described sand buries is 50-100 days, is preferably 60 days.
5., as the method as described in arbitrary in Claims 1-4, it is characterized in that: in described step (2), described sand buries as burying in vermiculite.
6., as the method as described in arbitrary in claim 1 to 5, it is characterized in that: in described step (3), the gibberellin concentration in Gibberellins solution is 1mM.
7., as the method as described in arbitrary in claim 1 to 6, it is characterized in that: in described step (3), the culture matrix of described cultivation is vermiculite.
8., as the method as described in arbitrary in claim 1 to 7, it is characterized in that: in described step (3), the temperature of described cultivation is 18-30 DEG C.
9., as the method as described in arbitrary in claim 1 to 8, it is characterized in that: the radix paeoniae rubrathe used is wild RADIX PAEONIAE RUBRA.
10. the application of arbitrary described method in radix paeoniae rubrathe expansion is numerous in claim 1 to 9.
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Cited By (8)
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| CN106561110A (en) * | 2016-11-03 | 2017-04-19 | 张晓峰 | Germination accelerating method of peony seeds |
| CN106852211A (en) * | 2016-12-08 | 2017-06-16 | 于绍震 | A kind of radix paeoniae rubrathe seed accelerating germination method |
| CN108401869A (en) * | 2018-01-31 | 2018-08-17 | 赤峰博康药业有限公司 | A kind of cultural method of northern area radix paeoniae rubra |
| CN108718596A (en) * | 2018-05-05 | 2018-11-02 | 内蒙古恒光大药业有限公司 | A method of improving radix paeoniae rubra seed germination rate and seedling percent |
| CN112136412A (en) * | 2020-10-08 | 2020-12-29 | 菏泽市国喜牡丹苗木有限公司 | Breeding method of peony seeds |
| CN113330851A (en) * | 2021-06-29 | 2021-09-03 | 河南省农业科学院园艺研究所 | Method for improving germination efficiency of peony seeds |
| CN114402736A (en) * | 2022-01-14 | 2022-04-29 | 内蒙古农业大学 | Method for industrially and rapidly growing seedlings of red paeony seeds |
| CN115004897A (en) * | 2022-04-06 | 2022-09-06 | 西南民族大学 | Germination method of red peony seeds |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106561110A (en) * | 2016-11-03 | 2017-04-19 | 张晓峰 | Germination accelerating method of peony seeds |
| CN106852211A (en) * | 2016-12-08 | 2017-06-16 | 于绍震 | A kind of radix paeoniae rubrathe seed accelerating germination method |
| CN108401869A (en) * | 2018-01-31 | 2018-08-17 | 赤峰博康药业有限公司 | A kind of cultural method of northern area radix paeoniae rubra |
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| CN113330851A (en) * | 2021-06-29 | 2021-09-03 | 河南省农业科学院园艺研究所 | Method for improving germination efficiency of peony seeds |
| CN114402736A (en) * | 2022-01-14 | 2022-04-29 | 内蒙古农业大学 | Method for industrially and rapidly growing seedlings of red paeony seeds |
| CN115004897A (en) * | 2022-04-06 | 2022-09-06 | 西南民族大学 | Germination method of red peony seeds |
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Application publication date: 20151209 |