CN114392350A - Hyaluronic acid oral liquid and preparation method thereof - Google Patents
Hyaluronic acid oral liquid and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a hyaluronic acid oral liquid and a preparation method thereof. The hyaluronic acid oral liquid comprises the following components in parts by weight: 5-10 parts of hyaluronic acid, 1-8 parts of collagen peptide, 0.5-3 parts of astaxanthin, 10-15 parts of peach gum extract, 5-10 parts of medlar extract, 1-5 parts of chia seed extract, 5-12 parts of honey, 2-9 parts of soybean lecithin, 0.1-0.5 part of potassium sorbate and 100 parts of water; wherein the molecular weight of the hyaluronic acid is 20 kDa-60 kDa; the molecular weight of the collagen peptide is 300-600 Da; the content of peach gum polysaccharide in the peach gum extract is 40-90%. The hyaluronic acid oral liquid disclosed by the invention is easy to absorb, has the functions of moisturizing, resisting wrinkles, whitening and the like, and is good in stability and good in taste and flavor.
Description
Technical Field
The invention relates to a hyaluronic acid oral liquid and a preparation method thereof.
Background
Hyaluronic acid, also called hyaluronic acid, belongs to mucopolysaccharide. The hyaluronic acid molecule can carry more than 500 times of water, and is one of the best moisture-preserving components recognized at present. 50% of hyaluronic acid exists in the skin, can play a moisturizing role on the skin by locking moisture, and the content of the hyaluronic acid in the human body directly influences the moisture content and the aging degree of the skin.
Astaxanthin, astaxanthin and astaxanthin. In 1933, a mauve crystal is extracted from aquatic products such as shrimp and crab, and is determined to be a carotenoid closely related to astaxanthin, so that the crystal is named as astaxanthin. It is widely found in shrimp, crab, fish, bird, some algae, fungi and other organisms. Astaxanthin, a non-provitamin a carotenoid, cannot be converted into vitamin a in the animal body, but has the same antioxidant effect as carotenoid, and its ability to quench singlet oxygen and capture free radicals is more than 10 times higher than that of beta-carotene and more than 100 times higher than that of vitamin E, which is also called super vitamin E. Astaxanthin has the characteristics of oxidation resistance, colorability and organism immunity enhancement, is allowed to be used as a food additive for production in countries such as the United states, and has wide development prospect as a natural food additive.
At present, hyaluronic acid is widely used in cosmetics for external use, and research on oral hyaluronic acid is relatively rare, especially an oral hyaluronic acid preparation or food having comprehensive efficacy. Develops a stable oral preparation with the respective effects of hyaluronic acid and astaxanthin and has great application value.
Disclosure of Invention
The invention aims to provide the hyaluronic acid oral liquid which is easy to absorb, has the functions of moisturizing, resisting wrinkles, whitening and the like, and has good taste and flavor. In the actual research and development process, the inventor finds that when the hyaluronic acid with the functions of moisturizing and resisting wrinkles is combined with the active ingredients with the functions of resisting oxidation and whitening, the hyaluronic acid cannot fully exert the respective effects, and the stability is poor. Through continuous trial and study, the inventor finally obtains the stable hyaluronic acid oral liquid with the functions of moisturizing, resisting wrinkles, whitening and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides hyaluronic acid oral liquid which comprises the following components in parts by weight: 5-10 parts of hyaluronic acid, 1-8 parts of collagen peptide, 0.5-3 parts of astaxanthin, 10-15 parts of peach gum extract, 5-10 parts of medlar extract, 1-5 parts of chia seed extract, 5-12 parts of honey, 2-9 parts of soybean lecithin, 0.1-0.5 part of potassium sorbate and 100 parts of water; wherein the molecular weight of the hyaluronic acid is preferably 20-60 kDa, and the molecular weight of the collagen peptide is 300-600 Da; the content of peach gum polysaccharide in the peach gum extract is 40-90%, and the percentage is the mass percentage of the peach gum polysaccharide in the peach gum extract.
In the present invention, the molecular weight of the hyaluronic acid is preferably 40kDa to 50 kDa.
In the present invention, the collagen peptide may be a low molecular weight collagen peptide. The molecular weight of the collagen peptide is preferably 400-500 Da.
In the present invention, the astaxanthin may be natural astaxanthin. The natural astaxanthin can be extracted from animal carapace, algae, bacteria, etc., such as Haematococcus pluvialis or shrimp shell.
In the present invention, the soybean lecithin generally refers to a mixture of various phospholipids extracted from soybean, and mainly includes phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, etc., which is also called soybean lecithin. In the present invention, the soybean lecithin functions as an emulsifier.
Preferably, the mass ratio of the astaxanthin to the soybean lecithin is 1: (1-5), for example 1: 2.
In the invention, the preparation method of the peach gum extract needs to ensure that the content of peach gum polysaccharide is 40-90%. In the research process, the inventor finds that when the peach gum extract contains the peach gum polysaccharide in the range, on one hand, the addition of the peach gum extract into the hyaluronic acid oral liquid can increase the viscosity of the oral liquid and is beneficial to improving the stability of the oral liquid; on the other hand, the addition of the peach gum extract can promote the absorption of hyaluronic acid and astaxanthin simultaneously.
Preferably, the content of peach gum polysaccharide in the peach gum extract is 75-90%.
Preferably, the preparation method of the peach gum extract comprises the following steps:
(1) adding water into peach gum raw materials to break the wall, and decocting to obtain peach gum slurry;
(2) filtering the peach gum slurry to obtain filtrate; centrifuging the filtrate to obtain a supernatant;
(3) concentrating the supernatant, and freeze-drying to obtain peach gum extract.
In the step (1), the wall breaking can be performed by a wall breaking machine. The mass ratio of the peach gum raw material to the water is preferably 1: (20 to 50).
In the step (1), the boiling temperature is preferably 90-100 ℃. The boiling time is preferably 1-4 h.
In the step (2), the filtration is preferably suction filtration.
In the step (2), the centrifugation can be performed by using a centrifuge. The rotation speed of the centrifugation is preferably 1000 to 2000 r/min.
In the step (3), the concentration is preferably heating concentration.
In the step (3), the temperature of the freeze drying is preferably-18 ℃ to-20 ℃.
In the preparation method, if the wall breaking is not performed in the step (1), the content of peach gum polysaccharide in the obtained peach gum extract is lower than 40%.
In the invention, the medlar extract can be obtained by extracting dry medlar with an acid solution. The acid solution is, for example, hydrochloric acid solution.
In the present invention, preferably, the preparation method of chia seed extract comprises the following steps:
(1) drying chia seeds at 65-80 ℃, and then grinding and sieving the dried chia seeds to obtain chia seed fine powder;
(2) carrying out reflux extraction on the chia seed fine powder by using an ethanol water solution with the concentration of 45-60%, filtering the filtrate obtained by each reflux extraction, and combining the filtrates;
(3) carrying out reduced pressure distillation on the filtrate to obtain a concentrated solution, adsorbing the concentrated solution by using macroporous adsorption resin, prewashing the concentrated solution by using purified water, then eluting the concentrated solution by using an ethanol water solution with the concentration of 45-60%, and collecting eluent;
(4) and (3) concentrating the eluent under reduced pressure, drying the eluent under reduced pressure at 60-75 ℃, and finally crushing the eluent to obtain the chia seed extract.
In the invention, the honey can be honey recorded in pharmacopoeia of the people's republic of China 2020 edition. In the present invention, the potassium sorbate may be food grade potassium sorbate, which functions as a preservative in the present invention.
In a preferred embodiment of the present invention, the hyaluronic acid is 8 to 10 parts, the collagen peptide is 1 to 3 parts, and the astaxanthin is 1 to 2 parts.
In a preferred embodiment of the present invention, the hyaluronic acid is 5 to 7 parts, the collagen peptide is 4 to 8 parts, and the astaxanthin is 2 to 3 parts.
In a preferred embodiment of the invention, the peach gum extract is 12-14 parts, the medlar extract is 6-8 parts, and the chia seed extract is 2-4 parts.
In a specific embodiment of the invention, the hyaluronic acid oral liquid comprises the following components in parts by weight: 9 parts of hyaluronic acid, 2 parts of collagen peptide, 1 part of astaxanthin, 13 parts of peach gum extract, 7 parts of medlar extract, 3 parts of chia seed extract, 10 parts of honey, 0.2 part of potassium sorbate and 100 parts of water.
In a specific embodiment of the invention, the hyaluronic acid oral liquid comprises the following components in parts by weight: 6 parts of hyaluronic acid, 8 parts of collagen peptide, 2.5 parts of astaxanthin, 14 parts of peach gum extract, 8 parts of Chinese wolfberry extract, 4 parts of chia seed extract, 12 parts of honey, 0.5 part of potassium sorbate and 100 parts of water.
In a specific embodiment of the invention, the hyaluronic acid oral liquid comprises the following components in parts by weight: 10 parts of hyaluronic acid, 3 parts of collagen peptide, 2 parts of astaxanthin, 14 parts of peach gum extract, 6 parts of medlar extract, 2 parts of chia seed extract, 6 parts of honey, 0.3 part of potassium sorbate and 100 parts of water.
The invention provides a preparation method of the hyaluronic acid oral liquid, which comprises the following steps:
s1, dissolving hyaluronic acid and collagen peptide in water, adding a mixture of astaxanthin and soybean lecithin, and homogenizing;
s2, adding the peach gum extract, the Chinese wolfberry extract, the chia seed extract, the honey and the potassium sorbate into the material obtained in the step S1, and homogenizing;
s3, sterilizing and filling to obtain the product.
In step S1, the dissolving temperature is preferably 40 to 65 ℃. The temperature of dissolution in the present invention refers to the temperature of water at the time of dissolution.
In step S1, preferably, the dissolving is performed under ultrasonic-assisted conditions, and the ultrasonic frequency is 30 to 50kHz during ultrasonic-assisted processing. More preferably, the collagen peptide is dissolved in water under the ultrasonic-assisted condition, and then hyaluronic acid is added for dissolution.
In steps S1 and S2, the homogenization may be performed using a high-pressure homogenizer.
In step S3, the sterilization is preferably performed by a high-voltage pulse electric field, wherein the field strength is 25-35 kV/cm, the pulse number is 200-400, and the pulse frequency is 150-350 Hz.
In step S3, the filling is preferably performed under aseptic conditions. The filled container may be a dark glass bottle. The filling dosage can be 20-100 mL, for example 50 mL.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows:
according to the invention, hyaluronic acid with low molecular weight, collagen with low molecular weight and astaxanthin are taken as core raw materials, and specific plant extracts are matched, so that the plant extracts can promote the absorption of the core raw materials, promote the full play of the effects of moisturizing, whitening, resisting wrinkles and the like of the core raw materials, and simultaneously improve the taste and flavor of the oral liquid; especially the peach gum extract can promote the absorption of core raw materials, improve the taste and flavor and is also beneficial to improving the stability of the oral liquid. The hyaluronic acid oral liquid can obviously improve the skin state, replenish water, preserve moisture, reduce fine wrinkles and delay aging; the oral liquid can enhance immunity and improve body constitution after long-term administration.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
In the following examples and comparative examples:
hyaluronic acid: the molecular weight is 40 kDa;
collagen peptide: molecular weight 400 Da;
astaxanthin: commercially available natural astaxanthin;
potassium sorbate: food grade potassium sorbate;
honey: honey recorded in pharmacopoeia of the people's republic of China 2020 edition one.
The preparation method of the peach gum extract comprises the following steps:
(1) adding 2L water into 100g of peach gum raw material (with impurities removed), breaking cell wall with a cell wall breaking machine, and decocting the obtained slurry at 100 deg.C for 3 hr (keeping boiling) to obtain peach gum slurry;
(2) carrying out suction filtration on the peach gum slurry to obtain filtrate; centrifuging the filtrate on a centrifuge at the rotating speed of 1800r/min to obtain supernatant;
(3) heating and concentrating the supernatant, and freeze-drying at-20 deg.C to obtain peach gum extract; the content of peach gum polysaccharide in the peach gum extract is 80%.
In addition, a comparative peach gum extract is also prepared, and the difference from the preparation is that the peach gum extract is obtained by directly decocting without wall breaking in the step (1), and the peach gum polysaccharide content in the peach gum extract is 30%.
The preparation method of the medlar extract comprises the following steps: extracting dried fructus Lycii with hydrochloric acid solution.
The preparation method of chia seed extract comprises the following steps:
(1) drying chia seeds at 70 ℃, and then grinding and sieving the dried chia seeds to obtain chia seed fine powder;
(2) reflux-extracting semen chianae fine powder with 60% ethanol water solution, filtering the filtrate obtained by each reflux extraction, and mixing the filtrates;
(3) distilling the filtrate under reduced pressure to obtain concentrated solution, adsorbing with macroporous adsorbent resin, pre-washing with purified water, eluting with 60% ethanol water solution, and collecting eluate;
(4) concentrating the eluate under reduced pressure, drying at 70 deg.C under reduced pressure, and pulverizing to obtain chia seed extract.
Example 1
The following components were weighed: 9g of hyaluronic acid, 2g of collagen peptide, 1g of astaxanthin, 13g of peach gum extract, 7g of medlar extract, 3g of chia seed extract, 10g of honey, 2g of soybean lecithin, 0.2g of potassium sorbate and 100g of water.
The hyaluronic acid oral liquid is prepared according to the following steps:
s1, dissolving collagen peptide in water at 50 ℃ under the ultrasonic-assisted condition, and then adding hyaluronic acid for dissolving; uniformly mixing astaxanthin and soybean lecithin, adding the obtained mixture into a solution of collagen peptide and hyaluronic acid, and homogenizing by using a high-pressure homogenizer;
s2, adding the peach gum extract, the Chinese wolfberry extract, the chia seed extract, the honey and the potassium sorbate into the material obtained in the step S1, and homogenizing by a high-pressure homogenizer;
s3, sterilizing by adopting a high-voltage pulse electric field, wherein the field intensity is 30kV/cm, the pulse number is 300, and the pulse frequency is 250 Hz; and (5) filling the mixture into a dark glass bottle with the volume of 50mL to obtain the product.
Example 2
The following components were weighed: 6g of hyaluronic acid, 8g of collagen peptide, 2.5g of astaxanthin, 14g of peach gum extract, 8g of Chinese wolfberry extract, 4g of chia seed extract, 12g of honey, 5g of soybean lecithin, 0.5g of potassium sorbate and 100g of water.
A hyaluronic acid oral liquid was prepared according to the method of example 1, with the only difference that: in step S1, the temperature of the water is 40 ℃.
Example 3
The following components were weighed: 10g of hyaluronic acid, 3g of collagen peptide, 2g of astaxanthin, 14g of peach gum extract, 6g of medlar extract, 2g of chia seed extract, 6g of honey, 2g of soybean lecithin, 0.3g of potassium sorbate and 100g of water. A hyaluronic acid oral liquid was prepared according to the method of example 1, with the only difference that: in step S1, the temperature of the water is 65 ℃.
Comparative example 1
The following components were weighed: 9g of hyaluronic acid, 2g of collagen peptide, 1g of astaxanthin, 7g of medlar extract, 3g of chia seed extract, 10g of honey, 2g of soybean lecithin, 0.2g of potassium sorbate and 100g of water. A hyaluronic acid oral liquid was prepared according to the method of example 1, and comparative example 1 did not contain peach gum extract, compared to example 1.
Comparative example 2
The following components were weighed: 9g of hyaluronic acid, 2g of collagen peptide, 1g of astaxanthin, 13g of peach gum extract, 7g of medlar extract, 10g of honey, 2g of soybean lecithin, 0.2g of potassium sorbate and 100g of water. A hyaluronic acid oral liquid was prepared according to the method of example 1, and comparative example 2 did not contain chia seed extract, compared to example 1.
Comparative example 3
The following components were weighed: 9g of hyaluronic acid, 2g of collagen peptide, 1g of astaxanthin, 13g of peach gum extract, 3g of chia seed extract, 10g of honey, 2g of soybean lecithin, 0.2g of potassium sorbate and 100g of water. A hyaluronic acid oral liquid was prepared according to the method of example 1, and comparative example 3 did not contain the lycium barbarum extract, compared to example 1.
Comparative example 4
The following components were weighed: 9g of hyaluronic acid, 2g of collagen peptide, 1g of astaxanthin, 13g of peach gum extract, 7g of medlar extract, 3g of chia seed extract, 10g of honey, 0.2g of potassium sorbate and 100g of water. In contrast to example 1, comparative example 4 contained no soy lecithin.
The hyaluronic acid oral liquid is prepared according to the following steps:
s1, dissolving collagen peptide in water at 50 ℃ under the ultrasonic-assisted condition, and then adding hyaluronic acid for dissolving; adding astaxanthin into a solution of collagen peptide and hyaluronic acid, and homogenizing by using a high-pressure homogenizer;
s2, adding the peach gum extract, the Chinese wolfberry extract, the chia seed extract, the honey and the potassium sorbate into the material obtained in the step S1, and homogenizing by a high-pressure homogenizer;
s3, sterilizing by adopting a high-voltage pulse electric field, wherein the field intensity is 30kV/cm, the pulse number is 300, and the pulse frequency is 250 Hz; and (5) filling the mixture into a dark glass bottle with the volume of 50mL to obtain the product.
Comparative example 5
The following components were weighed: 9g of hyaluronic acid, 2g of collagen peptide, 1g of astaxanthin, 13g of comparative peach gum extract, 7g of medlar extract, 3g of chia seed extract, 10g of honey, 2g of soybean lecithin, 0.2g of potassium sorbate and 100g of water. A hyaluronic acid oral liquid was prepared according to the method of example 1, and comparative example 1 used a comparative peach gum extract (peach gum polysaccharide content 30%) compared to example 1.
Effect example 1: stability test
Stability tests were performed on the hyaluronic acid oral liquids prepared in examples 1 to 3 and comparative examples 1 to 4, specifically: in one test, the hyaluronic acid oral liquid sample is kept at room temperature, and in the other test, the hyaluronic acid oral liquid sample is refrigerated at 4 ℃ and observed for change at 6h, 12h, 1d, 2d, 5d, 10d, 20d, 30d and 40 d. The observation results are shown in table 1, and it was found that the example samples of the present invention were always in a clear state and good in stability during the test. The hyaluronic acid oral liquid prepared in comparative example 4 has the worst stability, suspended matters appear after standing for 6 hours at room temperature, and precipitates appear after 1 day, so that the hyaluronic acid oral liquid cannot be taken; suspended matter appeared after 1 day at 4 ℃ and increased significantly at 5 days, and precipitation began to appear later. The hyaluronic acid oral liquid prepared in the comparative example 1 is poor in stability, suspended matters appear after the hyaluronic acid oral liquid is placed at room temperature for 12 hours, and precipitates appear after 2 days, so that the hyaluronic acid oral liquid cannot be taken; it is relatively stable when refrigerated at 4 deg.C, but suspended matter also appears at 40 d. The hyaluronic acid oral liquid prepared in the comparative example 5 is poor in stability, suspended matters appear after the hyaluronic acid oral liquid is placed at room temperature for 5 days, and precipitates appear after 20 days, so that the hyaluronic acid oral liquid cannot be taken; it is relatively stable when refrigerated at 4 deg.C, but suspended matter also appears at 40 d. Comparative examples 2 and 3 were relatively stable when refrigerated at 4 ℃, but suspended matter began to appear when left at room temperature for 40 days.
Table 1 stability test results
Effect example 2: microbiological indicator detection
According to the detection method (general rules 1105 and 1106) of microorganisms in medicines in the pharmacopoeia of the people's republic of China 2020 edition, the hyaluronic acid oral liquid prepared in the examples 1-3 and the comparative examples 1-3 is subjected to microorganism index detection, and the detection result is shown in table 2, and as can be seen from table 2, the hyaluronic acid oral liquid meets the microorganism limit standard (general rule 1107) of the oral liquid in the pharmacopoeia.
TABLE 2 microbiological indicator test results
Effect example 3: clinical investigation
80 adult female volunteers aged 30-50 years were divided into 8 groups of 10 persons each, including 3 experimental groups, 4 comparative groups and 1 control group. 3 groups of experimental groups were administered with the hyaluronic acid oral solutions of examples 1 to 3, and 3 groups of comparative groups were administered with the hyaluronic acid oral solutions of comparative examples 1 to 3 and 5, respectively, one bottle was administered 1 hour before sleep for 30 days continuously. The control group did not take any oral liquid. Throughout the experiment, the oral liquid was kept in a refrigerator at 4 ℃.
1. Skin moisture detection
Skin moisture was measured in 80 female volunteers before the start of the experiment, and again after the completion of the experiment, and the skin moisture measurement results are shown in table 3.
TABLE 3 comparison table of skin moisture content
| Moisture of skin | Before the experiment | After the experiment | Water content increase rate |
| Example 1 | 10 | 18 | 80％ |
| Example 2 | 10 | 15 | 50％ |
| Example 3 | 10 | 16 | 60％ |
| Comparative example 1 | 10 | 12 | 20％ |
| Comparative example 2 | 10 | 14 | 40％ |
| Comparative example 3 | 10 | 13 | 30％ |
| Comparative example 5 | 10 | 14 | 40％ |
| Control group | 10 | 10 | 0 |
The skin moisture content in table 3 was determined using a skin moisture test instrument (VAPO SCAN transdermal moisture loss meter), the moisture content values in table 3 are the average of the skin moisture content of 10 people in each group, and the moisture lifting rate was calculated by the method: (moisture content after experiment-moisture content before experiment)/moisture content before experiment 100%.
As can be seen from the experimental data in Table 3, the hyaluronic acid oral liquids prepared according to the examples all showed a moisture content increase of 50% or more, while the hyaluronic acid oral liquids prepared according to the comparative examples showed a moisture content increase of 20-40% and did not show any increase in the moisture content of the control group.
2. Investigation of skin improvement
After completion of the experiment, the skin improvement was investigated by questionnaire, and the number of volunteers showing improvement per group was counted, and the results are shown in table 4.
Table 4 comparative table of skin condition improvement
As can be seen from table 4, in the experimental group, the probability that the volunteers considered the skin wet to be improved reached 100%, the probability that the skin elasticity was improved to be 80% or more, the probability that the skin color was whitened to be 60% or more, and the probability that the skin as a whole was improved to be 100% after the administration of the hyaluronic acid oral liquid prepared according to the examples for 30 days continuously.
In the comparative group, using the hyaluronic acid oral liquid prepared according to the comparative example, after continuous administration for 30 days, volunteers considered that the probability of improvement in skin moisturization was 45%, the probability of improvement in skin elasticity was 35%, the probability of skin whitening was only 20%, and the probability of improvement in the whole skin was only 25%. The control group had no change in skin improvement.
Claims (10)
1. The hyaluronic acid oral liquid comprises the following components in parts by weight: 5-10 parts of hyaluronic acid, 1-8 parts of collagen peptide, 0.5-3 parts of astaxanthin, 10-15 parts of peach gum extract, 5-10 parts of medlar extract, 1-5 parts of chia seed extract, 5-12 parts of honey, 2-9 parts of soybean lecithin, 0.1-0.5 part of potassium sorbate and 100 parts of water; wherein the molecular weight of the hyaluronic acid is 20 kDa-60 kDa; the molecular weight of the collagen peptide is 300-600 Da; the content of peach gum polysaccharide in the peach gum extract is 40-90%, and the percentage is the mass percentage of the peach gum polysaccharide in the peach gum extract.
2. The hyaluronic acid oral liquid of claim 1, wherein the molecular weight of the hyaluronic acid is 40-50 kDa;
and/or the molecular weight of the collagen peptide is 400-500 Da;
and/or the peach gum polysaccharide content of the peach gum extract is 75-90%.
3. The hyaluronic acid oral liquid according to claim 1, wherein the hyaluronic acid is 8 to 10 parts, the collagen peptide is 1 to 3 parts, and the astaxanthin is 1 to 2 parts;
or 5-7 parts of hyaluronic acid, 4-8 parts of collagen peptide and 2-3 parts of astaxanthin.
4. The hyaluronic acid oral liquid of claim 1, wherein the peach gum extract is 12-14 parts;
and/or 6-8 parts of the wolfberry extract;
and/or 2-4 parts of chia seed extract;
and/or the mass ratio of the astaxanthin to the soybean lecithin is 1: (1-5).
5. The hyaluronic acid oral liquid of claim 1, wherein the preparation method of the peach gum extract comprises the following steps:
(1) adding water into peach gum raw materials to break the wall, and decocting to obtain peach gum slurry;
(2) filtering the peach gum slurry to obtain filtrate; centrifuging the filtrate to obtain a supernatant;
(3) concentrating the supernatant, and freeze-drying to obtain peach gum extract.
6. The hyaluronic acid oral liquid of claim 1, which comprises the following components in parts by weight: 9 parts of hyaluronic acid, 2 parts of collagen peptide, 1 part of astaxanthin, 13 parts of peach gum extract, 7 parts of medlar extract, 3 parts of chia seed extract, 10 parts of honey, 2 parts of soybean lecithin, 0.2 part of potassium sorbate and 100 parts of water;
or the hyaluronic acid oral liquid comprises the following components in parts by weight: 6 parts of hyaluronic acid, 8 parts of collagen peptide, 2.5 parts of astaxanthin, 14 parts of peach gum extract, 8 parts of Chinese wolfberry extract, 4 parts of chia seed extract, 12 parts of honey, 5 parts of soybean lecithin, 0.5 part of potassium sorbate and 100 parts of water;
or the hyaluronic acid oral liquid comprises the following components in parts by weight: 10 parts of hyaluronic acid, 3 parts of collagen peptide, 2 parts of astaxanthin, 14 parts of peach gum extract, 6 parts of medlar extract, 2 parts of chia seed extract, 6 parts of honey, 2 parts of soybean lecithin, 0.3 part of potassium sorbate and 100 parts of water.
7. A method for preparing the hyaluronic acid oral liquid of any of claims 1-6, comprising the steps of:
s1, dissolving hyaluronic acid and collagen peptide in water, adding a mixture of astaxanthin and soybean lecithin, and homogenizing;
s2, adding the peach gum extract, the Chinese wolfberry extract, the chia seed extract, the honey and the potassium sorbate into the material obtained in the step S1, and homogenizing;
s3, sterilizing and filling to obtain the product.
8. The method for preparing the hyaluronic acid oral liquid according to claim 7, wherein the dissolving temperature is 40-65 ℃ in step S1.
9. The method for preparing the hyaluronic acid oral liquid according to claim 7, wherein in step S1, the dissolving is performed under ultrasonic-assisted conditions, wherein the ultrasonic frequency is 30-50 kHz;
preferably, in step S1, the collagen peptide is dissolved in water under the ultrasound-assisted condition, and then the hyaluronic acid is added for dissolution.
10. The method of claim 7, wherein the step S3, the sterilizing step is performed by a high-voltage pulse electric field, the field strength is 25-35 kV/cm, the pulse number is 200-400, and the pulse frequency is 150-350 Hz.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693108A (en) * | 2009-10-09 | 2010-04-14 | 南京大渊美容保健有限公司 | Oral nutrition composition for beautifying skin |
| CN107095313A (en) * | 2017-05-05 | 2017-08-29 | 北京维斯卡特生物技术有限公司 | A kind of efficient absorption collagen type oral liquid |
| CN109010173A (en) * | 2018-09-06 | 2018-12-18 | 王国香 | A kind of multi-functional skin care is spraying and preparation method thereof |
| CN109260454A (en) * | 2018-11-15 | 2019-01-25 | 派能生物科技（深圳）有限公司 | A kind of preparation method and application for the composition for promoting skin collagen to generate |
| CN109771329A (en) * | 2019-03-08 | 2019-05-21 | 储殷 | Improve skin moisturizing function, composition of bowling anti-wrinkle and preparation method thereof |
| CN110464682A (en) * | 2018-05-10 | 2019-11-19 | 北京东方淼森生物科技有限公司 | A kind of peach gum composition, preparation method and application |
| CN110464691A (en) * | 2018-05-10 | 2019-11-19 | 北京东方淼森生物科技有限公司 | A kind of peach gum composition and its application |
| CN112568352A (en) * | 2020-02-29 | 2021-03-30 | 河北康平健康产业有限责任公司 | Anti-oxidation beauty-maintaining and young-keeping mulberry and lycium ruthenicum solid beverage and preparation method thereof |
| CN112998168A (en) * | 2021-04-23 | 2021-06-22 | 绿优品(福建)健康科技研发中心有限公司 | Hyaluronic acid composite polypeptide beverage and preparation method thereof |
| CN113116801A (en) * | 2019-12-26 | 2021-07-16 | 华熙生物科技股份有限公司 | Composition for improving skin sub-health state and preparation method thereof |
| CN113349373A (en) * | 2021-07-01 | 2021-09-07 | 海南三元星生物科技股份有限公司 | Astaxanthin-collagen peptide oral liquid and preparation method thereof |
| CN113813211A (en) * | 2021-10-29 | 2021-12-21 | 黄国庆 | A skin care product containing Ginseng radix extract and its preparation method |
-
2022
- 2022-01-25 CN CN202210088913.8A patent/CN114392350A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693108A (en) * | 2009-10-09 | 2010-04-14 | 南京大渊美容保健有限公司 | Oral nutrition composition for beautifying skin |
| CN107095313A (en) * | 2017-05-05 | 2017-08-29 | 北京维斯卡特生物技术有限公司 | A kind of efficient absorption collagen type oral liquid |
| CN110464682A (en) * | 2018-05-10 | 2019-11-19 | 北京东方淼森生物科技有限公司 | A kind of peach gum composition, preparation method and application |
| CN110464691A (en) * | 2018-05-10 | 2019-11-19 | 北京东方淼森生物科技有限公司 | A kind of peach gum composition and its application |
| CN109010173A (en) * | 2018-09-06 | 2018-12-18 | 王国香 | A kind of multi-functional skin care is spraying and preparation method thereof |
| CN109260454A (en) * | 2018-11-15 | 2019-01-25 | 派能生物科技（深圳）有限公司 | A kind of preparation method and application for the composition for promoting skin collagen to generate |
| CN109771329A (en) * | 2019-03-08 | 2019-05-21 | 储殷 | Improve skin moisturizing function, composition of bowling anti-wrinkle and preparation method thereof |
| CN113116801A (en) * | 2019-12-26 | 2021-07-16 | 华熙生物科技股份有限公司 | Composition for improving skin sub-health state and preparation method thereof |
| CN112568352A (en) * | 2020-02-29 | 2021-03-30 | 河北康平健康产业有限责任公司 | Anti-oxidation beauty-maintaining and young-keeping mulberry and lycium ruthenicum solid beverage and preparation method thereof |
| CN112998168A (en) * | 2021-04-23 | 2021-06-22 | 绿优品(福建)健康科技研发中心有限公司 | Hyaluronic acid composite polypeptide beverage and preparation method thereof |
| CN113349373A (en) * | 2021-07-01 | 2021-09-07 | 海南三元星生物科技股份有限公司 | Astaxanthin-collagen peptide oral liquid and preparation method thereof |
| CN113813211A (en) * | 2021-10-29 | 2021-12-21 | 黄国庆 | A skin care product containing Ginseng radix extract and its preparation method |
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