CN114286856A - 一种培养雨生红球藻生产虾青素的方法 - Google Patents

一种培养雨生红球藻生产虾青素的方法 Download PDF

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CN114286856A
CN114286856A CN202080060094.5A CN202080060094A CN114286856A CN 114286856 A CN114286856 A CN 114286856A CN 202080060094 A CN202080060094 A CN 202080060094A CN 114286856 A CN114286856 A CN 114286856A
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astaxanthin
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CN114286856B (zh
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郑玉彬
曹培鑫
刘帅帅
王�华
陈方见
谢诗懿
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SHANDONG JINJING BIOTECHNOLOGY Ltd
Shandong Baiang Biotechnology Co ltd
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Abstract

提供了一种生产虾青素的方法，包括：(a)获得产虾青素雨生红球藻的营养细胞；(b)在含有有机碳源的营养缺失的培养基中在无光照条件下异养培养所述产虾青素雨生红球藻的营养细胞，以获得孢子细胞；和(c)收获所述孢子细胞和/或虾青素，任选纯化虾青素。还提供了用于所述方法的培养基。

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PCT国内申请，说明书已公开。

Claims (18)

  1. PCT国内申请，权利要求书已公开。
CN202080060094.5A 2019-09-23 2020-09-22 一种培养雨生红球藻生产虾青素的方法 Active CN114286856B (zh)

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CN116622797A (zh) * 2023-04-03 2023-08-22 广州优卡思农业技术有限公司 一种从雨生红球藻提取虾青素的方法

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CN113717917A (zh) * 2021-06-30 2021-11-30 中国海洋大学 一种雨生红球藻纯种培养的联合抗生素施用方法
CN116891806B (zh) * 2023-07-07 2024-05-28 浙江大学 一种降低雨生红球藻死亡率的预胁迫处理方法
CN118028111B (zh) * 2024-03-15 2024-08-06 暨南大学 一种培养雨生红球藻的方法及应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004129504A (ja) * 2002-10-08 2004-04-30 Suntory Ltd アスタキサンチン含有脂質の製造方法
US20050214897A1 (en) * 2004-03-26 2005-09-29 Feng Chen Methods for production of astaxanthin from the green microalgae Chlorella in dark-heterotrophic cultures
CN103571906A (zh) * 2012-07-27 2014-02-12 上海泽元海洋生物技术有限公司 一种利用微藻高效生产虾青素的新方法
CN105420332A (zh) * 2015-12-10 2016-03-23 天津科技大学 一种雨生红球藻高产虾青素的方法
CN105755088A (zh) * 2016-05-18 2016-07-13 彭小伟 诱导雨生红球藻生产虾青素的方法
CN106399108A (zh) * 2016-09-28 2017-02-15 山东金晶生物技术有限公司 一种简便高效的雨生红球藻营养细胞培养和采收方法
CN107365826A (zh) * 2017-09-18 2017-11-21 深圳市德和生物科技有限公司 一种调控虾青素积累的方法
US20180002711A1 (en) * 2016-06-30 2018-01-04 Kuehnle Agrosystems, Inc. Heterotrophic production methods for microbial biomass and bioproducts

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4427167B2 (ja) * 2000-06-12 2010-03-03 新日本石油株式会社 カロテノイド色素の製法
EP1724357A4 (en) 2004-03-04 2010-11-17 Suntory Holdings Ltd METHOD FOR PRODUCING ASTAXANTHE-CONTAINING LIPIDS
JP2007097584A (ja) 2005-09-06 2007-04-19 Yamaha Motor Co Ltd アスタキサンチン含有量の高い緑藻およびその製造方法
CN106566775B (zh) * 2016-10-21 2020-04-07 青岛科海生物有限公司 含高活力雨生红球藻细胞制备方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004129504A (ja) * 2002-10-08 2004-04-30 Suntory Ltd アスタキサンチン含有脂質の製造方法
US20050214897A1 (en) * 2004-03-26 2005-09-29 Feng Chen Methods for production of astaxanthin from the green microalgae Chlorella in dark-heterotrophic cultures
CN103571906A (zh) * 2012-07-27 2014-02-12 上海泽元海洋生物技术有限公司 一种利用微藻高效生产虾青素的新方法
CN105420332A (zh) * 2015-12-10 2016-03-23 天津科技大学 一种雨生红球藻高产虾青素的方法
CN105755088A (zh) * 2016-05-18 2016-07-13 彭小伟 诱导雨生红球藻生产虾青素的方法
US20180002711A1 (en) * 2016-06-30 2018-01-04 Kuehnle Agrosystems, Inc. Heterotrophic production methods for microbial biomass and bioproducts
CN106399108A (zh) * 2016-09-28 2017-02-15 山东金晶生物技术有限公司 一种简便高效的雨生红球藻营养细胞培养和采收方法
CN107365826A (zh) * 2017-09-18 2017-11-21 深圳市德和生物科技有限公司 一种调控虾青素积累的方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KANG CD等: "Comparison of heterotrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis", 《APPL MICROBIOL BIOTECHNOL》, vol. 68, no. 02, pages 237 - 241, XP019331906, DOI: 10.1007/s00253-005-1889-2 *
WAISSMAN-LEVY N等: "Manipulation of trophic capacities in Haematococcus pluvialis enables low-light mediated growth on glucose and astaxanthin formation in the dark", 《ALGAL RESEARCH》, vol. 40, pages 1 - 13 *
张广伦等: "雨生红球藻中虾青素的研究与应用", 《中国野生植物资源》, vol. 38, no. 02, pages 72 - 77 *
张睿钦等: "雨生红球藻异养转化产虾青素的条件研究", 《浙江大学学报(农业与生命科学版)》, vol. 37, no. 06, pages 624 - 630 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116622797A (zh) * 2023-04-03 2023-08-22 广州优卡思农业技术有限公司 一种从雨生红球藻提取虾青素的方法

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EP4036216A1 (en) 2022-08-03
CN114286856B (zh) 2024-05-24
CA3153214A1 (en) 2021-04-01
JP2022548872A (ja) 2022-11-22
JP7535103B2 (ja) 2024-08-15
EP4036216A4 (en) 2023-10-18
WO2021057709A1 (zh) 2021-04-01

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