CN114158366A - Method for cutting propagation of chenopodium camellinum - Google Patents
Method for cutting propagation of chenopodium camellinum Download PDFInfo
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- CN114158366A CN114158366A CN202111325731.XA CN202111325731A CN114158366A CN 114158366 A CN114158366 A CN 114158366A CN 202111325731 A CN202111325731 A CN 202111325731A CN 114158366 A CN114158366 A CN 114158366A
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- chenopodium
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- 238000005520 cutting process Methods 0.000 title claims abstract description 53
- 241000219312 Chenopodium Species 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 29
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 18
- 239000011573 trace mineral Substances 0.000 claims abstract description 13
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 13
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 8
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 8
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 8
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 20
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 239000004576 sand Substances 0.000 claims description 11
- 239000002689 soil Substances 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 10
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 9
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 8
- 241001494479 Pecora Species 0.000 claims description 7
- 210000003608 fece Anatomy 0.000 claims description 7
- 239000010871 livestock manure Substances 0.000 claims description 7
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000012452 mother liquor Substances 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 4
- 229910018890 NaMoO4 Inorganic materials 0.000 claims description 4
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 4
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 4
- 239000011686 zinc sulphate Substances 0.000 claims description 4
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 3
- 239000005971 1-naphthylacetic acid Substances 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 229930003761 Vitamin B9 Natural products 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 229910052564 epsomite Inorganic materials 0.000 claims description 3
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 235000019159 vitamin B9 Nutrition 0.000 claims description 3
- 239000011727 vitamin B9 Substances 0.000 claims description 3
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052796 boron Inorganic materials 0.000 claims description 2
- 239000010941 cobalt Substances 0.000 claims description 2
- 229910017052 cobalt Inorganic materials 0.000 claims description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 2
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- 229910052750 molybdenum Inorganic materials 0.000 claims description 2
- 239000011733 molybdenum Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 241001234745 Camelina Species 0.000 claims 1
- 235000016401 Camelina Nutrition 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 18
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 5
- 241000282836 Camelus dromedarius Species 0.000 abstract description 3
- 240000006122 Chenopodium album Species 0.000 abstract description 2
- 235000009344 Chenopodium album Nutrition 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 description 7
- 244000265913 Crataegus laevigata Species 0.000 description 3
- 235000013175 Crataegus laevigata Nutrition 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000000442 meristematic effect Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000012882 rooting medium Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of plant cultivation, and relates to a method for cutting propagation of chenopodium camellinum, which comprises the following specific steps: (1) cutting off a chenopodium camellinum branch, and cutting the bottom of the chenopodium camellinum branch into a 45-degree cut; (2) soaking the branches in a rooting culture solution, wherein the formula of the rooting culture solution is as follows: IBA50-100mg/L + NAA 33-100mg/L + B950-150mg/L + ascorbic acid 0-110mg/L + BA 0-0.1mg/L + trace elements 0-1.5mg/L + macroelements 0-100 mg/L; (3) cutting the branches in the step (2) into a cutting medium, culturing for 15-20 days, and growing young roots on bark parts above the bottom cuts of the branches; the present invention provides a new breeding method of camel hair chenopodium album, and adoptsThe cutting propagation method can accelerate the propagation speed of the chenopodium camellinum, improve the survival rate of the chenopodium camellinum and provide theoretical and practical basis for rapid propagation of the chenopodium camellinum.
Description
The technical field is as follows:
the invention belongs to the technical field of plant cultivation, and particularly relates to a method for cutting propagation of chenopodium camellinum.
Background art:
chenopodium camellinum (scientific et Holmgren) is a perennial plant of the genus chenopodium, the family chenopodiaceae, the shrub plant. The plant height can reach 1m, and the branches are obliquely or flatly spread. The shape of the leaf strip, the shape of the strip are coated with needles, the shape of the needles or the shape of the rectangle are round, the male inflorescence is shorter, the flower tube has angular and longer splinters, and the length of the flower tube is 1/3 to the same length as the tube length. Erect fruit, oval, and hairy. 6-9 month flower and fruit period. Distributed in provinces such as Xinjiang, Tibet, Qinghai, Gansu and inner Mongolia in China; in the gobi, desert, semi-desert, arid hillside or grassland. The distribution is wide abroad and the distribution is in the thousand drought regions of the whole continental Eurasia. At present, the propagation method of the chenopodium hamiltonii is limited to seed planting, for example, Chinese patent CN201610019167.1 discloses a method for planting chenopodium hamiltonii in desert grassland of arid regions, which is completed by the steps of seed collection and storage, land selection and preparation, sowing and seedling management, water retaining ridges are arranged at hills between hills of the desert grassland, the seeds of the chenopodium hamiltonii are mixed with grass carbon soil and evenly mixed, and the seeds are sowed along the slope 20cm before the ridges, the method follows the occurrence rule of chenopodium hamiltonii seedlings to create the soil environment suitable for the seed germination and the seedling growth of the chenopodium hamiltonii, and the manual planting of the seedlings is realized; chinese patent CN201810840759.9 discloses a low-land replanting method between white thorn sand piles in a family pasture of a desertified grassland area, which comprises the following steps of firstly, selecting seeds, and placing the seeds in a seedbed for accelerating germination; step two, raising seedlings, namely raising the seedlings of the germinated seeds of the chenopodium manshurica in sandy loam; thirdly, preparing soil, digging planting holes in the low ground among the white thorn sand piles to be replanted, embedding a pouring pipe beside each row of planting holes, arranging a pouring hole communicated with each planting hole on each row of planting holes on each pouring pipe, arranging a water injection pipe with one end communicated with the pouring pipe, and communicating the other end of each water injection pipe extending out of the surface of the sand ground to a water storage tank arranged on the flat ground at the top of each white thorn sand pile; step four, transplanting; step five, field management, namely a method of first seedling cultivation and then transplanting, can ensure the survival rate of the seedlings after replanting; and through the pouring pipe that sets up and plant the cave intercommunication, realize the ground end and pour, the waste of water resource when avoiding the surface to spray. The prior art chenopodium hamiltonii is planted from seeds, the growth and the reproduction are slow, the survival rate is low, and the development of a method for rapidly reproducing the chenopodium hamiltonii is very necessary.
The invention content is as follows:
the invention aims to overcome the defects in the prior art and provide a method for cutting propagation of chenopodium camellinum, so as to solve the technical problem of low propagation efficiency of the current chenopodium camellinum.
In order to achieve the purpose, the invention provides a method for cutting propagation of chenopodium camellinum, which comprises the following specific steps:
(1) cutting 5-20cm of Chenopodium hamiltonii branches, and cutting the bottoms of the branches into 45-degree cuts;
(2) soaking the branches in a rooting culture solution for 10min, wherein the formula of the rooting culture solution is as follows: IBA50-100mg/L + NAA 33-100mg/L + B950-150mg/L + ascorbic acid (vitamin C)0-110mg/L + BA 0-0.1mg/L + trace element water solution 0-1.5mg/L + macroelement water solution 0-100 mg/L;
the trace element water solution contains manganese, molybdenum, cobalt, boron, zinc and copper elements; MnSO is dissolved in each 1L of microelement water solution4·H2O、ZnSO4·7H2O、H3BO3、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·5H2O; the preparation method of the trace element water solution comprises the following steps: respectively weighing MnSO4·H2O(16.9g)、ZnSO4·7H2O(8.6g)、H3BO3(6.2g)、NaMoO4·2H2O(0.25g)、CuSO4·5H2O (0.025g) and CoCl2·5H2O (0.025g) is dissolved by distilled water and has a constant volume of 1000mL to be a mother solution, and then 1.0mL of the mother solution has a constant volume of 1000mL to be the trace element aqueous solution in the invention.
The macroelement water solution contains anhydrous calcium chloride (CaCl)2) Magnesium sulfate heptahydrate (MgSO)4·7H2O, potassium nitrate (KNO)3) Ammonium Nitrate (NH)4NO3) And potassium dihydrogen phosphate (KH)2PO4) (ii) a The configuration method comprises the following steps: respectively weighing KNO3：76g、MgSO4·7H2O：14.8g、NH4NO3：66g、KH2PO4：6.8、CaCl2: 13.28g of the mother liquor is dissolved by distilled water and the volume is up to 2000 mL; then measuring 50mL of mother liquor to reach the constant volume of 1000mL, thus obtaining the macroelement aqueous solution in the invention.
The IBA is indolebutyric acid; NAA is 1-naphthylacetic acid, B9Is vitamin B9; 6-BA is 6-benzylaminopurine;
(3) cutting the branches obtained in the step (2) into a cutting medium of the chenopodium camellinum, wherein the cutting medium is sandy loam, the cutting depth of the branches is not more than 20cm, culturing the cut branches in the medium for 15-20 days, and growing young roots on bark parts above the cuts at the bottoms of the branches; culture conditions in the cutting process: shading with shading net to make the light intensity not higher than 5000Lx and the matrix humidity at 50-75%.
Preferably, the length of the branches in the step (1) is 8-10 cm.
Preferably, the rooting culture solution formula in the step (2) is IBA 100mg/L + NAA 33mg/L + B9100mg/L + ascorbic acid 110mg/L + BA 0.05mg/L + trace elements 1.0mg/L + macroelements 75 mg/L.
Preferably, the sandy loam in the step (3) is prepared from turfy soil, sheep manure and fine sand according to the mass ratio of 3: (1-3): (4-6).
Preferably, the sandy loam in the step (3) is prepared from turfy soil, sheep manure and fine sand according to the mass ratio of 3: 1: 6.
Preferably, the cutting depth of the step (3) is 6.0cm, and the stem node meristematic site is 3.0cm immersed into the substrate.
Compared with the prior art, the invention provides a novel method for breeding the chenopodium camellinum, the breeding speed of the chenopodium camellinum can be accelerated by adopting a cutting breeding method, the survival rate of the chenopodium camellinum is improved, and theoretical and practical basis is provided for rapid breeding of the chenopodium camellinum.
The specific implementation mode is as follows:
the invention is further illustrated by the following specific examples.
Example 1:
the embodiment relates to a method for cutting propagation of chenopodium camellinum, which comprises the following specific steps:
(1) cutting 5-20cm annual camel hair chenopodium album branches, and cutting the branches into cuttage branches with different lengths;
(2) and (2) soaking the branches obtained in the step (1) in a rooting culture solution for 10 minutes, wherein the formula of the rooting culture solution is as follows: IBA50-100mg/L + NAA 33-100mg/L + B950-150mg/L + ascorbic acid 0-110mg/L + BA 0-0.1mg/L + trace element aqueous solution 0-1.5mg/L + macroelement aqueous solution 0-100 mg/L;
the IBA is indolebutyric acid; NAA is 1-naphthylacetic acid, B9Is vitamin B9; 6-BA is 6-benzylaminopurine;
(3) cutting the branches processed in the step (2) into a substrate, and carrying out rooting culture, wherein the cutting substrate is sandy loam, and the sandy loam is prepared from turfy soil, sheep manure and fine sand according to the mass ratio of 3: (1-3): (4-6); culture conditions in the cutting process: shading by using a shading net to ensure that the illumination intensity is not higher than 5000Lx and the matrix humidity is optimal at 50-75%;
example 2:
the embodiment relates to a test of cuttage reproduction camel's hair chenopodium, specifically includes:
(1) influence of different cutting lengths on rooting rate and survival rate
By adopting the method of example 1, annual shoots were selected for the cuttings under otherwise unchanged conditions, and the effects of different cuttings lengths on rooting rate and survival rate are shown in table 1.
TABLE 1 influence of different cutting lengths on rooting and survival rates
| Length of cutting slip (cm) | Rooting percentage (%) | Survival rate (%) |
| 5-8cm | 30 | 35 |
| 8-10cm | 73 | 80 |
| 10-15cm | 43 | 46 |
| 15-20cm | 25 | 30 |
As can be seen from the table 1, when the length of the cuttage branch is 8-10.0cm, the rooting rate and survival rate of the chenopodium camellinum are the highest.
(2) Influence of different rooting culture solutions on rooting rate and survival rate
The results of the effects of different rooting medium formulations on the rooting rate and survival rate of the Chenopodium hamatum cutting seedlings under otherwise unchanged conditions by using the method of example 1 are shown in Table 2.
TABLE 2 influence of different rooting culture solutions on rooting rate and survival rate
As can be seen from Table 2, when the formulation of the rooting medium is IBA 100mg/L + NAA 33mg/L + B9When the content of the ascorbic acid in the mixture is 100mg/L, the content of the ascorbic acid in the mixture is 110mg/L, the content of BA in the mixture is 0.05mg/L, the content of the trace element water solution in the mixture is 1.0mg/L and the content of the macroelement water solution in the mixture is 75mg/L, the rooting rate and the survival rate of the chenopodium camellinn are the highest.
(3) Influence of cuttage depth and substrate proportion on root growth rate and survival rate
The results of the effect of different cutting depths and substrate on rooting rate and survival rate with the method of example 1 and under otherwise unchanged conditions are shown in table 3.
TABLE 3 influence of different cutting depths and substrates on rooting and survival rates
| Depth of cutting | Substrate (peat soil: sheep manure: fine sand) | Rooting rate | Survival rate |
| ＜5cm | 3：3：4 | 75％ | 5％ |
| 5-8cm | 3：3：4 | 10％ | 50％ |
| 8-10cm | 3：3：4 | 15％ | 70％ |
| 10-12cm | 3：3：4 | 20％ | 80％ |
| ＜5cm | 3：1：6 | 18％ | 65％ |
| 6.0cm | 3：1：6 | 80％ | 76％ |
| 8-10cm | 3：1：6 | 63％ | 76％ |
| 10-12cm | 3：1：6 | 35％ | 20％ |
As can be seen from Table 3, the cutting depth is best 6.0cm, and the stem node meristematic site is 3.0cm submerged into the substrate; the cuttage substrate comprises Chinese herbal carbon soil, sheep manure and fine sand according to the mass ratio of 3: 1: 6 hours, the rooting rate and survival rate of the chenopodium camellinum are highest.
Claims (9)
1. A method for cutting propagation of chenopodium hamatum is characterized by comprising the following specific steps:
(1) cutting 5-20cm of Chenopodium hamiltonii branches, and cutting the bottoms of the branches into 45-degree cuts;
(2) soaking the branches in a rooting culture solution for 10min, wherein the formula of the rooting culture solution is as follows: IBA50-100mg/L + NAA 33-100mg/L + B950-150mg/L + ascorbic acid 0-110mg/L + BA 0-0.1mg/L + trace element aqueous solution 0-1.5mg/L + macroelement aqueous solution 0-100 mg/L;
the trace element water solution contains manganese, molybdenum, cobalt, boron, zinc and copper elements; MnSO is dissolved in each 1L of microelement water solution4·H2O、ZnSO4·7H2O、H3BO3、NaMoO4·2H2O、CuSO4·5H2O、CoCl2·5H2O; the macroelement aqueous solution contains CaCl2、MgSO4·7H2O、KNO3、NH4NO3And KH2PO4；
The IBA is indolebutyric acid; NAA is 1-naphthylacetic acid, B9Is vitamin B9; 6-BA is 6-benzylaminopurine;
(3) and (3) cutting the branches obtained in the step (2) into a cutting medium of the chenopodium camellii, wherein the cutting medium is sandy loam, the cutting depth of the branches is not more than 20cm, culturing the cut branches in the medium for 15-20 days, and growing young roots on the bark above the bottom cut of the branches.
2. The method for cutting propagation of chenopodium camellinum as claimed in claim 1, wherein the culture conditions in the cutting process in step (3) are as follows: shading with shading net to make the light intensity not higher than 5000Lx and the matrix humidity at 50-75%.
3. The method for cutting propagation of chenopodium camellinum according to claim 1, wherein the length of the branches in the step (1) is 8-10 cm.
4. The method for cutting propagation of Chenopodium camelina of claim 1, wherein the formula of the rooting culture solution in the step (2) is IBA 100mg/L + NAA 33mg/L + B9100mg/L + ascorbic acid 110mg/L + BA 0.05mg/L + trace element aqueous solution 1.0mg/L + macroelement aqueous solution 75 mg/L.
5. The method for cutting propagation of chenopodium camellinum as claimed in claim 1, wherein the preparation method of the trace element aqueous solution comprises the following steps: respectively weighing MnSO4·H2O：16.9g、ZnSO4·7H2O：8.6g、H3BO3：6.2g、NaMoO4·2H2O：0.25g、CuSO4·5H2O: 0.025g and CoCl2·5H2O: 0.025g, dissolving with distilled water and fixing the volume to 1000mL to obtain mother liquor; then measuring 1.0mL of mother liquor to reach the constant volume of 1000mL, thus obtaining the trace element aqueous solution.
6. The cutting propagation method of chenopodium camellinum according to claim 1, characterized in that the configuration method comprises the following steps: respectively weighing KNO3：76g、MgSO4·7H2O：14.8g、NH4NO3：66g、KH2PO4：6.8、CaCl2: 13.28g of the mother liquor is dissolved by distilled water and the volume is up to 2000 mL; then measuring 50mL of mother liquor to reach the constant volume of 1000mL, thus obtaining the macroelement aqueous solution.
7. The cutting propagation method of Chenopodium hamiltonii according to claim 1, wherein the sandy loam in the step (3) is prepared from turfy soil, sheep manure and fine sand in a mass ratio of 3: (1-3): (4-6).
8. The cutting propagation method of Chenopodium hamiltonii according to claim 1, wherein the sandy loam in the step (3) is prepared from turfy soil, sheep manure and fine sand in a mass ratio of 3: 1: 6.
9. The method for cutting propagation of chenopodium camellinum as claimed in claim 1, wherein the cutting depth in step (3) is preferably 6.0cm, and the stem node meristem locus is submerged in the substrate by 3.0 cm.
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