CN1907001A - Cuttage reproducing method for cotton - Google Patents
Cuttage reproducing method for cotton Download PDFInfo
- Publication number
- CN1907001A CN1907001A CNA2005100889647A CN200510088964A CN1907001A CN 1907001 A CN1907001 A CN 1907001A CN A2005100889647 A CNA2005100889647 A CN A2005100889647A CN 200510088964 A CN200510088964 A CN 200510088964A CN 1907001 A CN1907001 A CN 1907001A
- Authority
- CN
- China
- Prior art keywords
- cotton
- branch
- root
- cuttage breeding
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a cuttage breeding method of cotton, which comprises the following steps: 1) grafting cotton bough in the culture medium of regenerated plant to breed root; or soaking cotton bough in the root-growing agent for 20-28h; plugging cotton in the soil-less base; 2) transplanting after the cotton bough grows root.
Description
Technical field
The present invention relates to fast numerous cultural method of plant, particularly relate to a kind of cuttage breeding method of cotton.
Background technology
The nutrition bowl seedling transplanting technology is one of major technique of using during Cotton in China is produced, is widely used in the cotton producing region in the Yangtze river basin and the Huanghe valley on the Soil Development in Huaibei Plain, transplants area throughout the year and accounts for the whole nation and plant 50% of cotton area, and area is about 4,500 ten thousand mu.But, there is following critical defect in this technology: the one, owing to adopt soil matrix matter carrier to grow seedlings, the seedbed area that needs is big, and the ratio of seedbed area and transplanting land for growing field crops area is about 1 in the production: 10-12, and the dispersing mode that also can only adopt single household of growing seedlings carries out; The 2nd, because soil matrix matter vehicle weight is big, get the seedling alms bowl that easily looses when transplanting, to carry and transplant time-consuming, transplanting can not adopt the mechanization mode to carry out, thereby production efficiency is lower; The 3rd, soil matrix replanting cotton takes root slowly, and seedling-slowing stage is long, also can have a negative impact to cotton prematurity.Therefore, soil matrix matter seedling transplanting technique has not been suitable for Cotton Production at present and in the future.
The research and production personnel of cotton transplant produce simple and direct, efficiently do a lot of work for realizing raising seedling of cotton, as the big alms bowl body of the matter of improving the soil is little alms bowl body, change above the average age for marriage seedling and are younger ones in an age group's seedling or bud transplantation of seedlings, transplant for convenience and also are adopted as reed pipe carrier etc.But above-mentioned measure all fails to realize no soil, factorial seedling growth and carrier-free, the novel mechanical transplanting of cotton, i.e. " two do not have two changes " technical goal.The Chinese Academy of Agricultural Sciences cotton carried out the development work of quick root-growing agent of cotton and the new matrix of Cultivation of Cotton Seedlings Without Soil in 2000 and obtained breakthrough, two national inventing patents have now been declared: " rooting promoter and its production and application ", number of patent application 02153630.9; " a kind of Cultivation of Cotton Seedlings Without Soil matrix ", number of patent application 03149367.X.In addition, realize " two change " corresponding cultivation techniques, formed " two do not have two changes " culture technique of cotton in conjunction with research " two do not have " raising seedling of cotton technology.This system is present state-of-the-art cotton factorial seedling growth system, but needs special-purpose seedling medium and cotton seeds, does not therefore also reach and utilizes medium and branch cutting technology to carry out the target of raising seedling of cotton merely.
The final election of cotton fine individual plant is the late growth stage in cotton, and this moment, plant was near dead, owing to selected the influence of environment, the effect that individual plant is selected is not as colony's reliability height, therefore, preserving fine individual plant and its asexual propagation is become a colony, also is that the cotton breeding worker craves for always.
Summary of the invention
The purpose of this invention is to provide a cuttage breeding method that grows cotton.
The cuttage breeding method of cotton provided by the present invention may further comprise the steps:
1) the cotton branch is received in the cotton regenerated seedling rooting medium expanded numerous taking root, or the base portion of cotton branch was soaked 20-28 hour with cotton branch cutting root-growing agent earlier, culture of rootage is carried out in cuttage in Cultivation of Cotton Seedlings Without Soil matrix again;
2) treat to transplant after the cotton branch grows root system, obtain cotton plants.
Described cotton regenerated seedling rooting culture medium prescription is to contain following substances: KNO in every 1000mL water
31.9-3.5g, NH
4NO
30.65-1.9g, MgSO
40.64-1.2g, KH
2PO
40.15-0.6g, CaCl
2H
2O0.3-1.2g, FeSO
47H
2O 0.2-0.4g, MnSO
44H
2O 0.017-0.085g, KI 0.004-0.02g, CuSO
40.00003-0.00006g, CoCl
20.00003-0.00006g, HBO
30.007-0.0095g, NAA 2-10mg, KT0.5-2mg, glutamine 0.4-0.8g, agar 6-8g, the pH value is 6.2-6.8.
For obtaining rooting efficiency preferably, also be added with trace mineral supplement in the described cotton regenerated seedling rooting medium, comprise in the following trace mineral supplement one or more: glycine 2mg, VB1 0.4mg, VB6 0.5mg, nicotinic acid 0.5mg and inositol 100mg.
The prescription of described cotton branch cutting root-growing agent is to contain following substances: KI0.004-0.02g, MnSO in every 1000mL water
44H
2O 0.017-0.085g, HBO
30.003-0.012g, ZnSO
47H
2O0.0043-0.035g, IAA 10-30mg, KT 1-10mg, 6-BA 1-10mg.
For obtaining rooting efficiency preferably, also be added with trace mineral supplement in the above-mentioned cotton branch cutting root-growing agent, comprise in the following trace mineral supplement one or more: VB1 0.2-0.8mg, VB6 0.2-0.8mg and inositol 75-150mg.
The prescription of described Cultivation of Cotton Seedlings Without Soil matrix and preparation method are referring to patent " a kind of Cultivation of Cotton Seedlings Without Soil matrix ", and number of patent application is: 03149367.X
The cotton branch received expand numerous condition of culture of taking root in the cotton regenerated seedling rooting medium and be: preserve moisture in confined conditions earlier and cultivated 12-20 days, and then astigmatism cultivates, can add the MS liquid nutrient medium according to needs in the incubation; Numerous cycle of described expansion is 50-60 days.
The cotton branch of culture of rootage is carried out in cuttage in Cultivation of Cotton Seedlings Without Soil matrix condition of culture is: the cultivation of preserving moisture earlier, this process can be bent in the canopy at plastic film and be carried out, and carries out hardening after 20-30 days.
In said method, the culture of rootage time is 55-65 days.
In addition, soak root with rooting promoter before transplanting, the foliage-spray antitranspirant can improve the survival rate of plant, and survival rate can reach more than 90%.
The cotton branch that available said method carries out the cuttage cultivation can be fruit branch, the vegetative shoot of cotton, the trophosomes such as bud or stem of going to live in the household of one's in-laws on getting married.
In addition, support plastic tunnel or the glass printing opacity room that can build certain area are the factory of growing seedlings, be beneficial to get light, heat, be incubated, also be convenient to ventilation and heat, and can be in conjunction with the growth of cereal crop seedlings, carry out light, temperature, water, fertile regulation and control, promote growing vigorously of cotton seedling, can place culturing rack in plastic tunnel or the greenhouse effectively to utilize space resources; Select for use the cotton branch of the anti-disease tolerant variety that is fit to local large tracts of land popularization to carry out cottage propagation with method of the present invention; Adopt farm machinery trench digging or burrow (cave) during transplanting, put seedling, add water and earthing integrated mechanical work, thereby form the asexual fast numerous and factorial seedling growth system of cotton, " asexual numerous soon " refers to utilize cotton branch cutting culture technique to carry out cotton fine individual plant, the asexual propagating technology of crossbreed, " factorial seedling growth " refers to utilize cotton branch cutting root media to cultivate the cotton seedling in a large number, replaces to produce to go up the cotton seed seedling transplanting technique of widely applying.The present invention can realize being substituted with soil matrix matter bed body with soilless culture medium; carrying out centralized protection with booth or greenhouse grows seedlings and replaces single household's hut dispersing mode protection and grow seedlings; being substituted with native carrier with the cotton transplantation of seedlings mode of carrier-free transplants; and, a degeneration of cotton improved seeds and a crossbreed needs production of hybrid seeds difficult problem have every year thoroughly been solved with the traditional seed germination training method of cotton branch culture of rootage replacement.
The present invention has following advantage: the one, the fast propagating seedling root that obtains with cotton branch quick-breeding method of the present invention is many, seedling strong, seedling diseases is few or do not have, get seedling after the root system preservation complete, favourable taking root fast returned seedling; The 2nd, practicable factorial seedling growth, the seedling medium quality does not have soil, and is in light weight, can carry out the batch production concentrated protection and grow seedlings, and risk is little, effective, saved seedlings growing area; The 3rd, the single transplantation of seedlings of practicable carrier-free, seedling carrier-free when soilless substrate bed body upper transplanting in addition, if use rooting promoter, then can be accelerated the transplanted seedling rooting rate, shortens the seedling time of returning, and improves planting percent; The 4th, practicable mechanization transplanting operation; The 5th, utilize branch cutting to replace cotton seed, solved the difficult problem that producing seeds of hybrid cotton cost height, improved seeds are easily degenerated.The present invention will play a significant role in the Cotton Production field.
Embodiment
Method therefor is conventional method if no special instructions among the following embodiment.
The cottage propagation of embodiment 1, cotton
Choose 5 strain cotton fine individual plants with the conventional method final election, each 5 of fruit branch and superfluous buds are got in every strain, receive in the cotton regenerated seedling rooting medium.Concrete grammar is: medium is divided in the 250mL triangular flask, and 125 ℃ of sterilizations 14 minutes are whittled into wedge shape with the cotton branch base portion of health and insert in the medium, seal with sealing film, cultivate on the illumination cultivation frame, remove after 20 days and seal the cultivation of film astigmatism.Shoot growth is 1 times after about 40 days, is transferred in the same medium again after cutting into 2 parts and expands numerous 1 time under same culture conditions, obtains suitable colony, the back transplant planting of taking root.The survival rate of statistics plant, the result is as shown in table 1:
The statistics of the cotton regenerated survival rate of plant of table 1
| Individual plant is compiled | 1 | 2 | 3 | 4 | 5 | |||||
| Branch source sampling number | Superfluous bud 5 | Fruit branch 5 | Superfluous bud 5 | Fruit branch 5 | Superfluous bud 5 | Fruit branch 5 | Superfluous bud 5 | Fruit branch 5 | Superfluous bud 5 | Fruit branch 5 |
| Expand a numerous field planting and survive survival rate | 12 9 83.3 | 13 11 84.6 | 11 10 90.9 | 14 12 85.7 | 11 10 90.9 | 13 11 84.6 | 13 12 92.3 | 13 9 69.2 | 12 8 75 | 13 9 92.3 |
Total survival rate of cotton regenerated plant reaches 84%, superfluous bud is numerous through expanding, take root after survival rate be 81.5%, fruit branch is numerous through expanding, take root after survival rate be 86.4%, show with cuttage breeding method of the present invention and can obtain cotton regenerated plant, survival rate is higher, and in addition, asexual propagation method of the present invention can satisfy the requirement of cotton breeding work, can realize that the batch production branch cutting of cotton grows seedlings, thereby can replace traditional hybrid seeding mode.
Claims (10)
1, a cuttage breeding method that grows cotton may further comprise the steps;
1) the cotton branch is received in the cotton regenerated seedling rooting medium expanded numerous taking root, or the base portion of cotton branch was soaked 20-28 hour with cotton branch cutting root-growing agent earlier, culture of rootage is carried out in cuttage in Cultivation of Cotton Seedlings Without Soil matrix again;
2) treat to transplant after the cotton branch grows root system, obtain cotton plants.
2, cuttage breeding method according to claim 1 is characterized in that: described cotton regenerated seedling rooting culture medium prescription is to contain following substances: KNO in every 1000mL water
31.9-3.5g, NH
4NO
30.65-1.9g, MgSO
40.64-1.2g, KH
2PO
40.15-0.6g, CaCl
2H
2O 0.3-1.2g, FeSO
47H
2O 0.2-0.4g, MnSO
44H
2O 0.017-0.085g, KI 0.004-0.02g, CuSO
40.00003-0.00006g, CoCl
20.00003-0.00006g, HBO
30.007-0.0095g, NAA 2-10mg, KT 0.5-2mg, glutamine 0.4-0.8g, agar 6-8g, the pH value is 6.2-6.8.
3, cuttage breeding method according to claim 2 is characterized in that: comprise in the following trace mineral supplement one or more in the described cotton regenerated seedling rooting medium: glycine 2mg, VB1 0.4mg, VB6 0.5mg, nicotinic acid 0.5mg and inositol 100mg.
4, according to claim 1 or 2 or 3 described cuttage breeding methods, it is characterized in that: the prescription of described cotton branch cutting root-growing agent is to contain following substances in every 1000mL water: KI 0.004-0.02g, MnSO
44H
2O0.017-0.085g, HBO
30.003-0.012g, ZnSO
47H
2O 0.0043-0.035g, IAA 10-30mg, KT1-10mg, 6-BA 1-10mg.
5, cuttage breeding method according to claim 4 is characterized in that: described cotton branch cutting root-growing agent comprises one or more in the following trace mineral supplement: VB1 0.2-0.8mg, VB6 0.2-0.8mg and inositol 75-150mg.
6, according to claim 1 or 2 or 3 described cuttage breeding methods, it is characterized in that: the described cotton branch is received expanded numerous condition of culture of taking root and be in the cotton regenerated seedling rooting medium: preserve moisture in confined conditions earlier and cultivated 12-20 days, astigmatism is cultivated again.
7, according to claim 1 or 2 or 3 described cuttage breeding methods, it is characterized in that: the cotton branch of culture of rootage is carried out in described cuttage in Cultivation of Cotton Seedlings Without Soil matrix condition of culture is: the cultivation of preserving moisture earlier, carry out hardening after 20-30 days; Numerous cycle of described expansion is 50-60 days.
8, according to claim 1 or 2 or 3 described cuttage breeding methods, it is characterized in that: the described culture of rootage time is 55-65 days.
9, according to claim 1 or 2 or 3 described cuttage breeding methods, it is characterized in that: soak root with rooting promoter, the foliage-spray antitranspirant before the described transplanting.
10, according to claim 1 or 2 or 3 described cuttage breeding methods, it is characterized in that: the fruit branch that described cotton branch is a cotton, vegetative shoot, superfluous bud or stem.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100889647A CN1907001A (en) | 2005-08-03 | 2005-08-03 | Cuttage reproducing method for cotton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100889647A CN1907001A (en) | 2005-08-03 | 2005-08-03 | Cuttage reproducing method for cotton |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1907001A true CN1907001A (en) | 2007-02-07 |
Family
ID=37698371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005100889647A Pending CN1907001A (en) | 2005-08-03 | 2005-08-03 | Cuttage reproducing method for cotton |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1907001A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102257919A (en) * | 2011-05-20 | 2011-11-30 | 湖南农业大学 | Node cutting propagation technology for cotton |
| CN101766087B (en) * | 2010-01-22 | 2012-02-01 | 中国农业科学院棉花研究所 | Method for open-air leafless cutting of Hainan cotton |
| CN102550605A (en) * | 2012-01-05 | 2012-07-11 | 南京轩凯生物科技有限公司 | Gamma-polyglutamic acid and naphthylacetic acid combined type rooting agent and using method thereof |
| CN102972297A (en) * | 2012-12-05 | 2013-03-20 | 中国农业科学院生物技术研究所 | Method for cultivating regeneration plants of cotton |
| CN106856953A (en) * | 2017-03-24 | 2017-06-20 | 合肥云都棉花有限公司 | One grows cotton cuttage root-taking matrix and its cottage method |
| CN109618698A (en) * | 2019-02-25 | 2019-04-16 | 福建省亚热带植物研究所 | A kind of Taiwan leaflet V. amurensis cuttage and seedling culture method |
| CN114158366A (en) * | 2021-11-10 | 2022-03-11 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for cutting propagation of chenopodium camellinum |
-
2005
- 2005-08-03 CN CNA2005100889647A patent/CN1907001A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766087B (en) * | 2010-01-22 | 2012-02-01 | 中国农业科学院棉花研究所 | Method for open-air leafless cutting of Hainan cotton |
| CN102257919A (en) * | 2011-05-20 | 2011-11-30 | 湖南农业大学 | Node cutting propagation technology for cotton |
| CN102550605A (en) * | 2012-01-05 | 2012-07-11 | 南京轩凯生物科技有限公司 | Gamma-polyglutamic acid and naphthylacetic acid combined type rooting agent and using method thereof |
| CN102972297A (en) * | 2012-12-05 | 2013-03-20 | 中国农业科学院生物技术研究所 | Method for cultivating regeneration plants of cotton |
| CN106856953A (en) * | 2017-03-24 | 2017-06-20 | 合肥云都棉花有限公司 | One grows cotton cuttage root-taking matrix and its cottage method |
| CN109618698A (en) * | 2019-02-25 | 2019-04-16 | 福建省亚热带植物研究所 | A kind of Taiwan leaflet V. amurensis cuttage and seedling culture method |
| CN114158366A (en) * | 2021-11-10 | 2022-03-11 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for cutting propagation of chenopodium camellinum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104067821B (en) | A kind of preparation method of Sweetpotato Viruses Elimination seedling | |
| CN1907001A (en) | Cuttage reproducing method for cotton | |
| CN105075863B (en) | A kind of Paeonia papaveracea rapid propagation method | |
| CN110692521A (en) | High-seedling-rate cultivation method for stem tissue culture of polygala tenuifolia | |
| CN114051927A (en) | Simplified breeding method for virus-free sweet potato seedlings | |
| CN102388801B (en) | Sterile grafting method of peanut tissue culture seedlings | |
| CN101946629A (en) | Method for rapidly obtaining pure line of hybrid wheat | |
| CN104304000A (en) | China fir clone tissue cultured seedling rooting induction method and rooting culture medium | |
| CN108377911A (en) | The tissue culture mating system of manglietia glauca | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN104012406A (en) | Regeneration in-vitro method for sweet cherry variety wanhongzhu | |
| CN109526743B (en) | Rapid propagation and fine variety breeding method for garlic callus | |
| CN100374012C (en) | Tissure culture and fast growth of buttercup flowers | |
| CN1203754C (en) | Breeding technology of test-tube konjac | |
| CN1042890C (en) | Plant growth regulator for beet | |
| CN103348915B (en) | Method for strong seedling and rooting culture of strawberries | |
| CN110235626A (en) | A kind of method that numerous bergamot pear is expanded in scale | |
| CN106857265B (en) | A kind of tissue culture enrichment procedure of white Cowhells wood | |
| CN103155866B (en) | Malus zumi tissue culture rapid propagation seedling raising method | |
| CN105145042A (en) | Soil cultivation method for potato virus-free basic seeds | |
| CN104488711A (en) | Method for obtaining intervarietal hybrids of eustoma grandiflorum rapidly | |
| CN1147220C (en) | Method of bacteria-free germination and fast propagation of peltate yam | |
| CN101889548A (en) | Cabbage haploid breeding method | |
| CN105103859B (en) | A kind of method for transplanting of peanut tissue culture seedlings | |
| CN112189516A (en) | Simple reproduction method for sympodial bamboo seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |