CN108271688A - A kind of oldenlandia diffusa Initial culture base and preparation method thereof - Google Patents
A kind of oldenlandia diffusa Initial culture base and preparation method thereof Download PDFInfo
- Publication number
- CN108271688A CN108271688A CN201711451046.5A CN201711451046A CN108271688A CN 108271688 A CN108271688 A CN 108271688A CN 201711451046 A CN201711451046 A CN 201711451046A CN 108271688 A CN108271688 A CN 108271688A
- Authority
- CN
- China
- Prior art keywords
- component
- concentration
- mother liquor
- great number
- oldenlandia diffusa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of oldenlandia diffusa Initial culture base, culture medium includes a great number of elements component, micro- component, molysite component, organic constituents, inositol, sucrose and agar powder；A great number of elements component includes：Potassium nitrate, ammonium nitrate, calcium chloride dihydrate, epsom salt and potassium dihydrogen phosphate；Trace element suite divides：Potassium iodide, boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate and cobalt chloride；Molysite component includes：Ferrous sulfate and disodium ethylene diamine tetraacetate；Organic constituents include：Niacin, puridoxine hydrochloride, thiamine hydrochloride and glycine.Oldenlandia grass seed is placed in above-mentioned culture medium, under suitable condition, oldenlandia grass seed can largely be sprouted rapidly, and germination rate is more than 95%, and seed germination rate is high, ensures the pure of kind.The invention also discloses a kind of preparation methods of oldenlandia diffusa Initial culture base, and flow is simple, easy to operate, and manufacturing cost is low.
Description
Technical field
The present invention relates to oldenlandia diffusa tissue cultivating and seedling technical field more particularly to a kind of oldenlandia diffusas to be just commissioned to train
Support base and preparation method thereof.
Background technology
Oldenlandia diffusa (Hedyotis diffusa Willd), which is that Rubiaceae is annual, hangs down loosely draft, high 15-50cm, root
It is elongated, branch, white flower.Stem is slightly rectangular or oblate cylindricality, Glabrous send out multi-branched from base portion, and in spring at florescence, kind is careful
Small yellowish-brown；It can receive for two seasons within 1 year, spring sowing August is received, and autumn planting is received November.It is grown in the area of 800 meters of height above sea level, is grown in water more
Field, ridge and moistening spaciously.It is mainly distributed on the areas such as domestic Fujian, Guangdong, Hong Kong, Guangxi, Hainan, Anhui, Yunnan；
Its bitter, light, cold in nature, primary efficacy is clearing heat and detoxicating, dispersing swelling and dissipating binds, promoting urination and removing dampness, the diseases such as venomous snake bite, and discovered in recent years is white
Flower HERBA HEDYOTIS DIFFUSAE has preferable effect, quantity larger cancer and hepatitis.
But oldenlandia diffusa is mostly wild, family's kind is seldom, and wild oldenlandia diffusa adulterant is more, there is corymbose hedyotis herb, fine flower
Auricled Hedyotis Herb, sealwort, sandwort, carpet weed, adulterant can be distinguished when fresh by tiny plant morphological characteristics, but dry product and seed are difficult
Distinguish the true and false.
And traditional Hedyotidis Diffusae breeding method is that oldenlandia grass seed is directly seeded into crop field, such sowing is deposited
In problems with：
(1) seed quality is lighter, and growing way can be influenced by sowing uneven, closeer place；
(2) big field weed is more, is mixed with adulterant kind and is easy to be doped into oldenlandia medicinal material or seed；
(3) crop field is by temperature, and the influence of humidity, germination rate and survival rate are relatively low.
Invention content
For overcome the deficiencies in the prior art, one of the objects of the present invention is to provide a kind of oldenlandia diffusa Initial cultures
Base is placed in oldenlandia grass seed in culture medium, is 24-26 DEG C, intensity of illumination 2000-2500lx in temperature, when illumination
Between under conditions of 10-12h/ days, oldenlandia grass seed can largely be sprouted rapidly, and germination rate, more than 95%, seed goes out
Bud rate is high, ensures the pure of kind.
The second object of the present invention is to provide a kind of preparation method of oldenlandia diffusa Initial culture base, and flow is simple,
Easy to operate, manufacturing cost is low.
An object of the present invention adopts the following technical scheme that realization：
A kind of oldenlandia diffusa Initial culture base, the culture medium include a great number of elements component, micro- component, molysite
Component, organic constituents, inositol, sucrose and agar powder；The a great number of elements component includes：Potassium nitrate, ammonium nitrate, two water chlorinations
Calcium, epsom salt and potassium dihydrogen phosphate；The trace element suite divides：Potassium iodide, boric acid, four water manganese sulfates, seven water sulphur
Sour zinc, sodium molybdate, cupric sulfate pentahydrate and cobalt chloride；The molysite component includes：Ferrous sulfate and disodium ethylene diamine tetraacetate；Institute
Stating organic constituents includes：Niacin, puridoxine hydrochloride, thiamine hydrochloride and glycine.
Further, the mass percentage concentration of a concentration of 96-100mg/L of inositol, sucrose are 2%, the concentration of agar powder
For 4.8g/L.
Further, the concentration of each component is as follows in component in a great number of elements：Potassium nitrate 1890-1900mg/L, nitre
Sour ammonium 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate 160-
170mg/L。
Further, the concentration of each component is as follows in the micro- component：Potassium iodide 0.8-0.83mg/L, boric acid
6.0-6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, white vitriol 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L,
Cupric sulfate pentahydrate 0.025-0.028mg/L, cobalt chloride 0.028-0.03mg/L.
Further, the concentration of each component is as follows in the molysite component：Ferrous sulfate 27.5-29mg/L, ethylenediamine tetraacetic
Acetic acid disodium 23-25mg/L.
Further, the concentration of each component is as follows in the organic constituents：Niacin 0.2-0.3mg/L, puridoxine hydrochloride
0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, glycine 0.8-0.9mg/L.
Further, pH is adjusted to 5.8-6.0 by the culture medium with acid-base indicator.
The second object of the present invention adopts the following technical scheme that realization：
A kind of preparation method of oldenlandia diffusa Initial culture base, including：
A great number of elements mother liquor preparation process：A great number of elements component is taken respectively, is configured to a great number of elements mother liquor, a large amount of members
The concentration of each component is 10-20 times of respective formula concentration in plain mother liquor；
Micro- mother liquor preparation process：Micro- component is taken respectively, is configured to micro- mother liquor, the micro member
The concentration of each component is 10 times of respective formula concentration in plain mother liquor；
Mother liquid of iron salt preparation process：Molysite component is taken respectively, is configured to mother liquid of iron salt, each component in the mother liquid of iron salt
Concentration is 10-20 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic constituents are taken respectively, are configured to organic matter mother liquor, in the organic matter mother liquor
The concentration of each component is 10-20 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Mix constant volume heating stepses：Take a great number of elements mother liquor 20-30ml, micro- mother liquor 10-15ml, molysite female respectively
Liquid 10-15ml, organic matter mother liquor 10-15ml, inositol solution, agar powder 3.5-4.8g, sucrose 20-40g, mixing, wait for that sucrose is molten
After solution it is fixed it is molten arrive 1L, then heating is until agar powder melts, and then dispenses to culture bottle, after cooled and solidified to obtain the final product.
Further, further include sterilization steps：Heating is dispensed into culture bottle, every bottle of 30- until agar powder thawing
40ml covers tightly bottle cap, culture bottle is put into the autoclave that temperature is 121-125 DEG C, pressure is 0.15-0.3MPa, and sterilize 20-
30min, sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, after cooled and solidified to obtain the final product.
Further, in a great number of elements mother liquor preparation process：In addition to calcium chloride dihydrate individually carries out preparing storage,
Remaining component mixed preparing in a great number of elements component.
Compared with prior art, the beneficial effects of the present invention are：
(1) oldenlandia diffusa Initial culture base provided by the present invention, component are easy to get, and formula is reasonable, oldenlandia diffusa
Seed is placed in the culture medium, is 24-26 DEG C, intensity of illumination 2000-2500lx in temperature, light application time is 10-12h/ days
Under conditions of, oldenlandia grass seed can largely be sprouted rapidly, and germination rate is more than 95%, and seed germination rate is high, obtains pure
After net oldenlandia diffusa seedling, the breeding in sawdust is planted, you can obtain certified products oldenlandia diffusa and seed, guaranty
That plants is pure.
(2) preparation method of oldenlandia diffusa Initial culture base provided by the present invention, flow is simple, easy to operate, system
It is standby at low cost.
Specific implementation mode
In the following, in conjunction with specific implementation mode, the present invention is described further, it should be noted that is do not collided
Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.
A kind of oldenlandia diffusa Initial culture base, including a great number of elements component (potassium nitrate, ammonium nitrate, calcium chloride dihydrate, seven
Water magnesium sulfate and potassium dihydrogen phosphate), micro- component (potassium iodide, boric acid, four water manganese sulfates, white vitriol, sodium molybdate,
Cupric sulfate pentahydrate and cobalt chloride), molysite component (ferrous sulfate and disodium ethylene diamine tetraacetate), organic constituents (niacin, hydrochloric acid
Pyridoxol, thiamine hydrochloride and glycine), inositol, sucrose and agar powder.
As further embodiment, the concentration of each component is as follows in component in a great number of elements：Potassium nitrate 1890-
1900mg/L, ammonium nitrate 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, phosphoric acid
Potassium dihydrogen 160-170mg/L；The concentration of each component is as follows in micro- component：Potassium iodide 0.8-0.83mg/L, boric acid 6.0-
6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, white vitriol 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, five water
Copper sulphate 0.025-0.028mg/L, cobalt chloride 0.028-0.03mg/L；The concentration of each component is as follows in molysite component：Sulfuric acid is sub-
Iron 27.5-29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L；The concentration of each component is as follows in organic constituents：Niacin 0.2-
0.3mg/L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, glycine 0.8-0.9mg/L；Inositol
A concentration of 96-100mg/L, the mass percentage concentration of sucrose is 2%, a concentration of 4.8g/L of agar powder.
As further embodiment, pH is adjusted to 5.8-6.0 by culture medium with acid-base indicator.
The oldenlandia diffusa Initial culture base that the embodiment of the present invention is provided, component are easy to get, and formula is reasonable, oldenlandia
Grass seed is placed in the culture medium, is 24-26 DEG C, intensity of illumination 2000-2500lx, light application time 10-12h/ in temperature
Under conditions of it, oldenlandia grass seed can largely be sprouted rapidly, and germination rate is more than 95%, and seed germination rate is high, obtains
After pure oldenlandia diffusa seedling, the breeding in sawdust is planted, you can obtain certified products oldenlandia diffusa and seed, ensure
Kind it is pure.
A kind of preparation method of oldenlandia diffusa Initial culture base, including：
A great number of elements mother liquor preparation process：A great number of elements component is taken respectively, is configured to a great number of elements mother liquor, and a great number of elements is female
The concentration of each component is 10-20 times of respective formula concentration in liquid；
Micro- mother liquor preparation process：Micro- mother liquor is taken respectively, is configured to micro- mother liquor, and trace element is female
The concentration of each component is 10 times of respective formula concentration in liquid；
Mother liquid of iron salt preparation process：Mother liquid of iron salt component is taken respectively, is configured to mother liquid of iron salt, each component in mother liquid of iron salt
Concentration is 10-20 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic matter mother liquor component is taken respectively, is configured to organic matter mother liquor, in organic matter mother liquor
The concentration of each component is 10-20 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Mix constant volume heating stepses：Take a great number of elements mother liquor 20-30ml, micro- mother liquor 10-15ml, molysite female respectively
Liquid 10-15ml, organic matter mother liquor 10-15ml, inositol solution, agar powder 3.5-4.8g, sucrose 20-40g, mixing, wait for that sucrose is molten
After solution it is fixed it is molten arrive 1L, then heating is until agar powder melts, and then dispenses to culture bottle, after cooled and solidified to obtain the final product.
Further include sterilization steps as further embodiment：Heating is dispensed into culture bottle until agar powder thawing
In, every bottle of 30-40ml covers tightly bottle cap, and culture bottle is put into the autoclave that temperature is 121-125 DEG C, pressure is 0.15-0.3MPa
In, sterilize 20-30min, and sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, after cooled and solidified to obtain the final product.
As further embodiment, in a great number of elements mother liquor preparation process：Except calcium chloride dihydrate is individually matched
System storage is outer, remaining component mixed preparing in a great number of elements component.Because calcium chloride dihydrate mixes easily with other a great number of elements
Precipitation, individually storage can effectively avoid this problem.
As further embodiment, when preparing a great number of elements mother liquor, the dosage difference of each component is as follows：Potassium nitrate
370-390g, ammonium nitrate 320-340g, calcium chloride dihydrate 80-95g, epsom salt 70-75g, potassium dihydrogen phosphate 30-35g.
As further embodiment, when preparing micro- mother liquor, the dosage difference of each component is as follows：Potassium iodide
1.0-1.66g, boric acid 11.0-12.4g, four water manganese sulfate 40-46g, white vitriol 17.0-18.0g, sodium molybdate 0.2-
0.5g, cupric sulfate pentahydrate 0.05-0.06g, cobalt chloride 0.05-0.06g.
As further embodiment, when preparing mother liquid of iron salt, the dosage difference of each component is as follows：Ferrous sulfate
20-25g, disodium ethylene diamine tetraacetate 21-26g.
As further embodiment, when preparing organic matter mother liquor, the dosage difference of each component is as follows：Niacin 1.0-
1.5g, puridoxine hydrochloride 1.25-1.75g, thiamine hydrochloride 1.0-1.5g, glycine 4.0-4.5g.
As further embodiment, in inositol solution preparation process, 0.2-0.3g inositols are taken, it is sterile with 100ml
Water dissolution, obtains inositol solution, and inositol solution is now with the current.
The preparation method for the oldenlandia diffusa Initial culture base that the embodiment of the present invention is provided, flow is simple, easy to operate,
Manufacturing cost is low.
It is specific embodiment of the present invention below, used raw material, equipment etc. remove special limit in the following embodiments
It can be obtained by buying pattern outside fixed.
Embodiment 1-5
A kind of oldenlandia diffusa Initial culture base, including a great number of elements component (potassium nitrate, ammonium nitrate, calcium chloride dihydrate, seven
Water magnesium sulfate and potassium dihydrogen phosphate), micro- component (potassium iodide, boric acid, four water manganese sulfates, white vitriol, sodium molybdate,
Cupric sulfate pentahydrate and cobalt chloride), molysite component (ferrous sulfate and disodium ethylene diamine tetraacetate), organic constituents (niacin, hydrochloric acid
Pyridoxol, thiamine hydrochloride and glycine), inositol, sucrose and agar powder, culture medium pH is adjusted to 5.8- with acid-base indicator
6.0.Concentration of the above-mentioned each component in the Initial culture base is as shown in table 1 below.
The formula table of the oldenlandia diffusa Initial culture base of 1 embodiment 1-5 of table
The oldenlandia diffusa Initial culture base of embodiment 1-5 is prepared in accordance with the following methods：
A great number of elements mother liquor preparation process：A great number of elements component is taken respectively, except calcium chloride dihydrate individually carries out preparation storage
Outside, remaining component mixed preparing in a great number of elements component obtains a great number of elements mother liquor, and each component is dense in a great number of elements mother liquor
Degree is 10 times of respective formula concentration；
Micro- mother liquor preparation process：Micro- mother liquor is taken respectively, is configured to micro- mother liquor, and trace element is female
The concentration of each component is 10 times of respective formula concentration in liquid；
Mother liquid of iron salt preparation process：Mother liquid of iron salt component is taken respectively, is configured to mother liquid of iron salt, each component in mother liquid of iron salt
Concentration is 10 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic matter mother liquor component is taken respectively, is configured to organic matter mother liquor, in organic matter mother liquor
The concentration of each component is 10 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Mix constant volume heating stepses：Suitable a great number of elements mother liquor, micro- mother liquor, mother liquid of iron salt, organic is taken respectively
Object mother liquor, inositol solution, agar powder and sucrose, mixing, fixed molten to 1L after sucrose dissolving, then heating is until agar powder melts
Change, be dispensed into culture bottle, every bottle of 30-40ml covers tightly bottle cap, by culture bottle be put into temperature be 123 DEG C, pressure 0.25MPa
Autoclave in, sterilize 25min, and sterilizing is finished and taken the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, after cooled and solidified i.e.
.
Effect assessment and performance detection
Purebred oldenlandia grass seed is taken, takes traditional direct-seeding to carry out nursery respectively, and using embodiment 1-5's
Culture medium carries out nursery, and the condition of two kinds of training methods is：Temperature is 24-26 DEG C, intensity of illumination 2000-2500lx, light
It it is 10-12h/ days according to the time.Test result is recorded, as a result as shown in table 2 below.
2 oldenlandia grass seed of table carries out the logging table of tradition live streaming and culture medium tissue culture
It can be obtained from the record of table 2, oldenlandia grass seed carries out tissue culture using the Initial culture base of embodiment 1-5,
Sprout time is significantly smaller than traditional direct-seeding seedling raising manners, and germination rate and survival rate are both significantly higher than traditional direct-seeding,
And it is 0% that the adulterant of embodiment 1-5, which obscures rate, the Initial culture base of embodiment 1-5 can effectively ensure that oldenlandia grass product
That plants is pure.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (10)
1. a kind of oldenlandia diffusa Initial culture base, which is characterized in that the culture medium includes a great number of elements component, trace element
Component, molysite component, organic constituents, inositol, sucrose and agar powder；The a great number of elements component includes：Potassium nitrate, nitric acid
Ammonium, calcium chloride dihydrate, epsom salt and potassium dihydrogen phosphate；The trace element suite divides：Potassium iodide, boric acid, four water sulphur
Sour manganese, white vitriol, sodium molybdate, cupric sulfate pentahydrate and cobalt chloride；The molysite component includes：Ferrous sulfate and ethylenediamine tetraacetic
Acetic acid disodium；The organic constituents include：Niacin, puridoxine hydrochloride, thiamine hydrochloride and glycine.
2. oldenlandia diffusa Initial culture base as described in claim 1, which is characterized in that a concentration of 96-100mg/ of inositol
The mass percentage concentration of L, sucrose are 2%, a concentration of 4.8g/L of agar powder.
3. oldenlandia diffusa Initial culture base as claimed in claim 2, which is characterized in that each in component in a great number of elements
The concentration of component is as follows：Potassium nitrate 1890-1900mg/L, ammonium nitrate 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L,
Epsom salt 370-375mg/L, potassium dihydrogen phosphate 160-170mg/L.
4. oldenlandia diffusa Initial culture base as claimed in claim 3, which is characterized in that each group in the trace element component
The concentration divided is as follows：Potassium iodide 0.8-0.83mg/L, boric acid 6.0-6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, seven water
Zinc sulfate 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.028mg/L, cobalt chloride 0.028-
0.03mg/L。
5. oldenlandia diffusa Initial culture base as claimed in claim 4, which is characterized in that each component in the molysite component
Concentration is as follows：Ferrous sulfate 27.5-29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L.
6. oldenlandia diffusa Initial culture base as claimed in claim 5, which is characterized in that each component in the organic constituents
Concentration it is as follows：Niacin 0.2-0.3mg/L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, sweet ammonia
Sour 0.8-0.9mg/L.
7. oldenlandia diffusa Initial culture base as described in claim 1, which is characterized in that the culture medium acid-base indicator
PH is adjusted to 5.8-6.0.
8. a kind of preparation method of oldenlandia diffusa Initial culture base as described in claim 6 any one, which is characterized in that
Including：
A great number of elements mother liquor preparation process：A great number of elements component is taken respectively, is configured to a great number of elements mother liquor, and a great number of elements is female
The concentration of each component is 10-20 times of respective formula concentration in liquid；
Micro- mother liquor preparation process：Micro- component is taken respectively, is configured to micro- mother liquor, and the trace element is female
The concentration of each component is 10 times of respective formula concentration in liquid；
Mother liquid of iron salt preparation process：Molysite component is taken respectively, is configured to mother liquid of iron salt, the concentration of each component in the mother liquid of iron salt
It is 10-20 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic constituents are taken respectively, are configured to organic matter mother liquor, each group in the organic matter mother liquor
The concentration divided is 10-20 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Mix constant volume heating stepses：A great number of elements mother liquor 20-30ml, micro- mother liquor 10-15ml, mother liquid of iron salt are taken respectively
10-15ml, organic matter mother liquor 10-15ml, inositol solution, agar powder 3.5-4.8g, sucrose 20-40g, mixing wait for that sucrose dissolves
Afterwards it is fixed it is molten arrive 1L, then heating is until agar powder melts, and then dispenses to culture bottle, after cooled and solidified to obtain the final product.
9. the preparation method of oldenlandia diffusa Initial culture base as claimed in claim 8, which is characterized in that further include sterilizing step
Suddenly：Heating is dispensed into culture bottle, every bottle of 30-40ml covers tightly bottle cap, and culture bottle, which is put into temperature, is until agar powder thawing
121-125 DEG C, in the autoclave that pressure is 0.15-0.3MPa, sterilize 20-30min, and sterilizing is finished and taken the dish out of the pot, and tightens culture bottle cap
And it is put into culture medium storage room, after cooled and solidified to obtain the final product.
10. the preparation method of oldenlandia diffusa Initial culture base as claimed in claim 8, which is characterized in that described a large amount of
In element mother liquor preparation process：In addition to calcium chloride dihydrate individually carries out preparing storage, remaining component in a great number of elements component is mixed
It closes and prepares.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711451046.5A CN108271688A (en) | 2017-12-27 | 2017-12-27 | A kind of oldenlandia diffusa Initial culture base and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711451046.5A CN108271688A (en) | 2017-12-27 | 2017-12-27 | A kind of oldenlandia diffusa Initial culture base and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108271688A true CN108271688A (en) | 2018-07-13 |
Family
ID=62802514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711451046.5A Pending CN108271688A (en) | 2017-12-27 | 2017-12-27 | A kind of oldenlandia diffusa Initial culture base and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108271688A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158366A (en) * | 2021-11-10 | 2022-03-11 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for cutting propagation of chenopodium camellinum |
-
2017
- 2017-12-27 CN CN201711451046.5A patent/CN108271688A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 张海洋等: "抗癌植物白花蛇舌草生物学特性及栽培技术", 《北方园艺》 * |
| 李国平等: "白花蛇舌草叶片离体培养及试管无性系的建立", 《广西植物》 * |
| 郭巧生等: "白花蛇舌草种子萌发特性", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158366A (en) * | 2021-11-10 | 2022-03-11 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for cutting propagation of chenopodium camellinum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110301318B (en) | Planting method of selenium-rich peanuts | |
| CN107896958A (en) | The water planting implantation methods of selenium-rich romaine lettuce | |
| CN106748165A (en) | A kind of liquid fertilizer and its production method containing humic acid | |
| CN103798145A (en) | Culture medium for tissue culture of vernonia amygdalina del. | |
| CN107047316A (en) | A kind of iris tissue culture method and culture medium | |
| CN102884981A (en) | Zanthoxylum nitidum tissue culture medium | |
| CN103798144A (en) | Culture medium for tissue culture of toona ciliate roem. | |
| CN103451147A (en) | Lactuca sativa L. pollen in vitro germination culture medium and method for measuring pollen activity | |
| CN101811902A (en) | Manufacturing process of multifunctional enriched-selenium plant nutrient | |
| CN106508757A (en) | Method for imitative-ecological breeding of Whitmania pigra through paddy field net cages | |
| CN106577245A (en) | Aeroponic culture method for polygonatum odoratum | |
| CN110149868A (en) | Reduce the preparation and its method of Cd concentration of brown rice | |
| CN108271688A (en) | A kind of oldenlandia diffusa Initial culture base and preparation method thereof | |
| CN108353787A (en) | A kind of pleione bulbocodioides Initial culture base and preparation method thereof | |
| CN104982113A (en) | Method for planting Suaeda salsa in medium-/low-copper saline-alkaline land and extracting plant salt | |
| CN107624634A (en) | A kind of method of black chrysanthemum seed growing | |
| CN111742933A (en) | Method for improving pollination fertilization capability of litchi in flowering phase | |
| CN110338023A (en) | A kind of method of Chinese prescription cultivation lima bean | |
| CN108124704A (en) | A kind of oldenlandia diffusa subculture medium and preparation method thereof | |
| CN108142291A (en) | A kind of oldenlandia diffusa root media and preparation method thereof | |
| CN104642107A (en) | Tissue culture propagation method for dendrobium huoshanense | |
| CN108323434A (en) | A kind of lycium ruthenicum root media and preparation method thereof | |
| CN102805032B (en) | Method for preventing daemonorops margaritae callus browning phenomena from occurring | |
| CN108901691A (en) | A kind of implantation methods of organic Se-rich rice | |
| CN105660392A (en) | Test tube culture method of tubers of pinellia ternata |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190402 Address after: 650000 6th Floor, Block A, Sunshine Building, 999 Kegao Road, Wuhua District, Kunming City, Yunnan Province Applicant after: Kangmei Pharmaceutical (Kunming) Germplasm Resources Co., Ltd. Address before: 663099 Wenshan Zhong and Miao Autonomous Prefecture Wenshan City, Yunnan Province, Kaihua South Road Wenshan Biovalley of Chinese Traditional Medicine 4-21 Applicant before: Kangmei Pharmaceutical (Wenshan) medicinal material planting Management Co., Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180713 |
|
| RJ01 | Rejection of invention patent application after publication |