CN108353787A - A kind of pleione bulbocodioides Initial culture base and preparation method thereof - Google Patents
A kind of pleione bulbocodioides Initial culture base and preparation method thereof Download PDFInfo
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- CN108353787A CN108353787A CN201711451055.4A CN201711451055A CN108353787A CN 108353787 A CN108353787 A CN 108353787A CN 201711451055 A CN201711451055 A CN 201711451055A CN 108353787 A CN108353787 A CN 108353787A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of pleione bulbocodioides Initial culture base and preparation method thereof, pleione bulbocodioides Initial culture base is 1/2MS culture mediums, and the sucrose of 2.0g/L, the agar powder of 4.8g/L are added in the 1/2MS culture mediums.Initial culture base using the present invention carries out primary tissue cultures advantage and is to overcome seed embryo hypoplasia, under natural environment the problems such as low reproduction rate；The speed and breeding quantity of pleione bulbocodioides seedling breeding can be effectively improved, the stability of germplasm is controlled from maternal source, ensures seedling quality, realizes the factorial praluction of pleione bulbocodioides high quality seed seedling, meet ornamental, medicinal demand, reduces the case where wild resource spreads unchecked excavation.
Description
Technical field
The present invention relates to a kind of tissue cultivating and seedling technology more particularly to a kind of pleione bulbocodioides Initial culture base and its preparation sides
Method.
Background technology
Pleione bulbocodioides Pleione bulbocodioides (Franch.) Rolfe, orchid family Pleion, ground life or grass of growing nonparasitically upon another plant
This.It is grown in the hayashishita or border stony ground of 1100-3500 meters of height above sea level or on the rock of moss covering, is also common in careless slope slightly
The gravel that the moon covers is on the ground.It is mainly distributed on the Sichuan Province China west and south (in Yuexi, Yanyuan, asbestos, Xichang, wood), WESTERN GUIZHOU extremely
Northern (Kerry, Yin Jiang, Leishan, Kweiyang, Mount Fanjing, Pan county, Anlong, Wangmo County, Nayong), northwestern Yunnan Province to the southeast (Gong Shan,
Wei Xi, Lijing, Dali, Yangbi, Yongning, Mengzi, mountain of papers) and Southeastern Tibet (Cha Walong), Upper Myanmar is also distributed.With root
Stem is used as medicine, and has effects that clearing heat and detoxicating, Sweeling-eliminating medicine powder section, preventing phlegm from forming and stopping coughing, is chiefly used in asthma and cough and arthroncus, venomous snake bite
Treatment etc..Pleione bulbocodioides pattern uniqueness is beautiful, and there is higher ornamental value, scape to be sent out from the old pseudobulb base portion of no leaf,
Uprightly, one flower of top tool, flower purple, lavender, pink or near-white sometimes have purple or dark red color spot, flower on lip
Month phase 4-5, the fruiting period 9-10 months.
The pleione bulbocodioides to circulate currently on the market is mostly wild, and minority is artificial cultivation；Pleione bulbocodioides breeding is mostly division propagation,
Pollen pollination rate is smaller in the natural environment, and the quantity for growing fruit pod is also less, and seed embryo hypoplasia can only be in aseptic condition
Under be seeded in culture medium and carry out tissue cultures.
Invention content
For overcome the deficiencies in the prior art, one of the objects of the present invention is to provide a kind of pleione bulbocodioides Initial culture bases.
The advantages of Initial culture base progress tissue cultures using the present invention, is to overcome seed embryo hypoplasia, be bred under natural environment
The problems such as rate is low；The speed and breeding quantity that pleione bulbocodioides seedling breeding can be effectively improved, the steady of germplasm is controlled from maternal source
It is qualitative, ensure seedling quality, realize the factorial praluction of pleione bulbocodioides high quality seed seedling, meet ornamental, medicinal demand, reduces wild
The case where excavation is spread unchecked in production-goods source.
The second object of the present invention is to provide a kind of preparation method of pleione bulbocodioides Initial culture base.
An object of the present invention adopts the following technical scheme that realization：A kind of pleione bulbocodioides Initial culture base is cultivated for 1/2MS
Base is added to the sucrose of 2.0g/L, the agar powder of 4.8g/L in the 1/2MS culture mediums.
Further, the 1/2MS culture mediums are grouped as by following group：Potassium nitrate 1890-1900mg/L, ammonium nitrate
1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate 160-
170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 6.0-6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, white vitriol
8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.028mg/L, cobalt chloride 0.028-0.03mg/
L, ferrous sulfate 27.5-29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L, inositol 96-100mg/L, niacin 0.2-0.3mg/
L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, glycine 0.8-0.9mg/L.
Further, the 1/2MS culture mediums are grouped as by following group：Potassium nitrate 1895mg/L, ammonium nitrate 1645mg/L,
Calcium chloride dihydrate 442.5mg/L, epsom salt 372.5mg/L, potassium dihydrogen phosphate 165mg/L, potassium iodide 0.82mg/L, boric acid
6.1mg/L, four water manganese sulfate 22.7mg/L, white vitriol 8.8mg/L, sodium molybdate 0.22mg/L, cupric sulfate pentahydrate
0.026mg/L, cobalt chloride 0.029mg/L, ferrous sulfate 28mg/L, disodium ethylene diamine tetraacetate 24mg/L, inositol 98mg/L, cigarette
Sour 0.25mg/L, puridoxine hydrochloride 0.3mg/L, thiamine hydrochloride 0.25mg/L, glycine 0.85mg/L.
The second object of the present invention adopts the following technical scheme that realization：A kind of preparation method of pleione bulbocodioides Initial culture base,
Including,
The prewired step of solution：A great number of elements solution, trace element solution, iron salt solutions, organic component solution are prepared respectively；
Mixing step：By a great number of elements solution, trace element solution, iron salt solutions, organic component solution, sucrose and agar
Powder mixes, and constant volume, sterilizing, cooling, solidification are to get pleione bulbocodioides Initial culture base.
Further, in the prewired step of solution, the preparation method of a great number of elements solution is as follows：Take the nitre of formula ratio
Sour potassium, ammonium nitrate, calcium chloride dihydrate, epsom salt, potassium dihydrogen phosphate dissolve respectively, then potassium nitrate, ammonium nitrate, seven water sulphur
The container of the containers store of sour magnesium, potassium dihydrogen phosphate mixing constant volume to 10L, calcium chloride dihydrate constant volume to 10L individually stores, standby
With.
Further, in the prewired step of solution, the preparation method of the trace element solution is as follows：Take the iodine of formula ratio
Change potassium, boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate, cobalt chloride, mixing constant volume arrives after dissolving respectively
It is stored in the container of 5L, it is spare.
Further, in the prewired step of solution, the preparation method of the iron salt solutions is as follows：Take the sulfuric acid of formula ratio sub-
After iron, disodium ethylene diamine tetraacetate dissolving in mixing constant volume to the brown bottle of 5L, it is kept in dark place, it is spare.
Further, in the prewired step of solution, the preparation method of the organic component solution is as follows：Take the cigarette of formula ratio
It is stored for future use in constant volume to the container of 5L after acid, puridoxine hydrochloride, thiamine hydrochloride, glycine dissolving.
Further, the concrete operations of the mixing step are as follows：The a great number of elements solution, micro of formula ratio is measured respectively
Element Solution, iron salt solutions, organic component solution be added 1L container in, then by 0.2-0.3g inositols, 2g/L sucrose,
After the agar powder dissolving of 4.8g/L in mixing constant volume to the container of above-mentioned 1L, container is heated to agar powder and is melted, by culture medium
Be dispensed into 30-40ml/ bottles of culture bottle, cover tightly bottle cap, by culture bottle be put into temperature be 121-125 DEG C, pressure 0.15-0.3MPa
Sterilising conditions under sterilize 20-30min, sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, waits for that its cooling is solidifying
Admittedly being Initial culture base.
Compared with prior art, the beneficial effects of the present invention are：
Initial culture base using the present invention carries out primary tissue cultures advantage and is to overcome seed embryo hypoplasia, natural
Under environment the problems such as low reproduction rate；The speed and breeding quantity that pleione bulbocodioides seedling breeding can be effectively improved, are controlled from maternal source
The stability of production of hybrid seeds matter ensures seedling quality, realizes the factorial praluction of pleione bulbocodioides high quality seed seedling, meets ornamental, medicinal
Demand reduces the case where wild resource spreads unchecked excavation.Tissue cultures provide suitable humiture, illumination item for pleione bulbocodioides simultaneously
Part, sufficient nutritional ingredient increase light application time, increase the speed of growth of plant, ensure the survival rate of plant.
Specific implementation mode
In the following, in conjunction with specific implementation mode, the present invention is described further, it should be noted that is do not collided
Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.
In the present invention, if not refering in particular to, all parts, percentage are unit of weight, used equipment and raw material etc.
It is commercially available or commonly used in the art.Method in following embodiments is unless otherwise instructed the normal of this field
Rule method.
A kind of pleione bulbocodioides Initial culture base is 1/2MS culture mediums, be added in the 1/2MS culture mediums 2.0g/L sucrose,
The agar powder of 4.8g/L.
As further embodiment, the 1/2MS culture mediums are grouped as by following group：Potassium nitrate 1890-1900mg/
L, ammonium nitrate 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate
160-170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 6.0-6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, seven water sulphur
Sour zinc 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.028mg/L, cobalt chloride 0.028-
0.03mg/L, ferrous sulfate 27.5-29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L, inositol 96-100mg/L, niacin
0.2-0.3mg/L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, glycine 0.8-0.9mg/L.
As most preferred embodiment, the 1/2MS culture mediums are grouped as by following group：Potassium nitrate 1895mg/L, nitre
Sour ammonium 1645mg/L, calcium chloride dihydrate 442.5mg/L, epsom salt 372.5mg/L, potassium dihydrogen phosphate 165mg/L, potassium iodide
0.82mg/L, boric acid 6.1mg/L, four water manganese sulfate 22.7mg/L, white vitriol 8.8mg/L, sodium molybdate 0.22mg/L, five water
Copper sulphate 0.026mg/L, cobalt chloride 0.029mg/L, ferrous sulfate 28mg/L, disodium ethylene diamine tetraacetate 24mg/L, inositol
98mg/L, niacin 0.25mg/L, puridoxine hydrochloride 0.3mg/L, thiamine hydrochloride 0.25mg/L, glycine 0.85mg/L.
The preparation method of the pleione bulbocodioides Initial culture base, including,
The prewired step of solution：A great number of elements solution, trace element solution, iron salt solutions, organic component solution are prepared respectively；
Mixing step：By a great number of elements solution, trace element solution, iron salt solutions, organic component solution, sucrose and agar
Powder mixes, and constant volume, sterilizing, cooling, solidification are to get pleione bulbocodioides Initial culture base.
As further embodiment, in the prewired step of solution, the preparation method of a great number of elements solution is as follows：
Take potassium nitrate, ammonium nitrate, calcium chloride dihydrate, epsom salt, the potassium dihydrogen phosphate of formula ratio to dissolve respectively, then potassium nitrate,
The containers store of ammonium nitrate, epsom salt, potassium dihydrogen phosphate mixing constant volume to 10L, the container of calcium chloride dihydrate constant volume to 10L
Individually storage, it is spare.Calcium chloride, which is mixed into other a great number of elements, easy tos produce precipitation, so individually storage.
As further embodiment, in the prewired step of solution, the preparation method of the trace element solution is as follows：
The potassium iodide of formula ratio, boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate, cobalt chloride are taken, is dissolved respectively
It mixes and is stored in constant volume to the container of 5L afterwards, it is spare.
As further embodiment, in the prewired step of solution, the preparation method of the iron salt solutions is as follows：It takes and matches
After ferrous sulfate, the disodium ethylene diamine tetraacetate just measured dissolve in mixing constant volume to the brown bottle of 5L, it is kept in dark place, it is spare.
As further embodiment, in the prewired step of solution, the preparation method of the organic component solution is as follows：
It is stored for future use in constant volume to the container of 5L after taking niacin, puridoxine hydrochloride, thiamine hydrochloride, the glycine of formula ratio to dissolve.
As further embodiment, the concrete operations of the mixing step are as follows：The a large amount of of formula ratio are measured respectively
Element Solution, trace element solution, iron salt solutions, organic component solution be added 1L container in, then by 0.2-0.3g inositols,
After the agar powder dissolving of the sucrose, 4.8g/L of 2g/L in mixing constant volume to the container of above-mentioned 1L, container is heated to agar powder and is melted
Change, culture medium is dispensed into 30-40ml/ bottles of culture bottle, covers tightly bottle cap, culture bottle is put into temperature is 121-125 DEG C, pressure is
Sterilize 20-30min under the sterilising conditions of 0.15-0.3MPa, and sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage
Room waits for that its cooled and solidified is Initial culture base.
It is specific embodiment of the present invention below, used raw material, equipment etc. remove special limit in the following embodiments
It can be obtained by buying pattern outside fixed.
Embodiment 1-3Pleione bulbocodioides Initial culture base
The proportioning according to the form below 1 weighs raw material respectively, is prepared in accordance with the following steps, the difference is that added
Raw material proportioning is different, prepares pleione bulbocodioides Initial culture base product, specifically refers to table 1：
Table 1：The raw material proportioning table of embodiment 1-3
The preparation method of above-mentioned pleione bulbocodioides Initial culture base, including,
The prewired step of solution：A great number of elements solution, trace element solution, iron salt solutions, organic component solution are prepared respectively；
It is specific as follows：The preparation method of a great number of elements solution is as follows：Take the potassium nitrate of formula ratio, ammonium nitrate, calcium chloride dihydrate, seven
Water magnesium sulfate, potassium dihydrogen phosphate dissolve respectively, and then potassium nitrate, ammonium nitrate, epsom salt, potassium dihydrogen phosphate mixing constant volume arrive
The container of the containers store of 10L, calcium chloride dihydrate constant volume to 10L individually stores, spare.The preparation side of the trace element solution
Method is as follows：The potassium iodide of formula ratio, boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate, cobalt chloride are taken,
It is stored in mixing constant volume to the container of 5L after dissolving respectively, it is spare.The preparation method of the iron salt solutions is as follows：Take formula ratio
After ferrous sulfate, disodium ethylene diamine tetraacetate dissolving in mixing constant volume to the brown bottle of 5L, it is kept in dark place, prevents from aoxidizing, it is spare.
The preparation method of the organic component solution is as follows：Take niacin, puridoxine hydrochloride, thiamine hydrochloride, the glycine of formula ratio molten
It is stored for future use in constant volume to the container of 5L after solution.
Mixing step：Concrete operations are as follows：A great number of elements solution, trace element solution, the molysite of formula ratio are measured respectively
Solution, organic component solution are added in the container of 1L, then by 0.2-0.3g inositols, the agar powder of the sucrose of 2g/L, 4.8g/L
It is mixed in constant volume to the container of above-mentioned 1L after dissolving, container, which is heated to agar powder, to be melted, and culture medium is dispensed into culture bottle 30-
40ml/ bottles, bottle cap is covered tightly, culture bottle is put under the sterilising conditions that temperature is 121-125 DEG C, pressure is 0.15-0.3MPa and is gone out
Bacterium 20-30min, sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, waits for that its cooled and solidified is Initial culture
Base.
Effect assessment and Indexs measure
Culture medium tissue culture is carried out to pleione bulbocodioides using the pleione bulbocodioides Initial culture base of embodiment 1-3, with traditional straight
It is comparative example 1 to connect and be seeded into the mode nursery in crop field, and every group of pleione bulbocodioides seed amount is 100, is referred to cultivation results items
Mark carries out statistical result such as table 2：
The pleione bulbocodioides Initial culture base of 2 embodiment 1-3 of table and the live streaming formula of comparative example carry out culture of rootage to pleione bulbocodioides
Result
| Sprout time (day) | Germination rate (%) | Survival rate (%) | |
| Comparative example 1 | 60 | 30% | 25% |
| Embodiment 1 | 30 | 85% | 70% |
| Embodiment 2 | 25 | 95% | 75% |
| Embodiment 3 | 35 | 88% | 73% |
Traditional pleione bulbocodioides breeding method is that pleione bulbocodioides seed is directly seeded into crop field, live streaming there is a problem of it is very much,
Unevenness is broadcasted sowing, growing way is bad；It is influenced by temperature, humidity, the speed of growth is slow.Initial culture base using the present invention carries out just
It is to overcome seed embryo hypoplasia for tissue cultures advantage, under natural environment the problems such as low reproduction rate；It can effectively improve solely
The speed and breeding quantity of garlic orchid seedling breeding, the stability of germplasm is controlled from maternal source, ensures seedling quality, realizes only garlic
The factorial praluction of blue high quality seed seedling meets ornamental, medicinal demand, reduces the case where wild resource spreads unchecked excavation.Simultaneously
Tissue cultures provide suitable humiture, illumination condition for pleione bulbocodioides, and sufficient nutritional ingredient increases light application time, increases and plants
The speed of growth of strain, ensures the survival rate of plant.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (9)
1. a kind of pleione bulbocodioides Initial culture base, which is characterized in that be 1/2MS culture mediums, be added in the 1/2MS culture mediums
The sucrose of 2.0g/L, the agar powder of 4.8g/L.
2. pleione bulbocodioides Initial culture base as described in claim 1, which is characterized in that the 1/2MS culture mediums are by following component
Composition：Potassium nitrate 1890-1900mg/L, ammonium nitrate 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt
370-375mg/L, potassium dihydrogen phosphate 160-170mg/L, potassium iodide 0.8-0.83mg/L, boric acid 6.0-6.2mg/L, four water sulfuric acid
Manganese 22.3-23.0mg/L, white vitriol 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-
0.028mg/L, cobalt chloride 0.028-0.03mg/L, ferrous sulfate 27.5-29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L,
Inositol 96-100mg/L, niacin 0.2-0.3mg/L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L,
Glycine 0.8-0.9mg/L.
3. pleione bulbocodioides Initial culture base as claimed in claim 2, which is characterized in that the 1/2MS culture mediums are by following component
Composition：Potassium nitrate 1895mg/L, ammonium nitrate 1645mg/L, calcium chloride dihydrate 442.5mg/L, epsom salt 372.5mg/L, phosphorus
Acid dihydride potassium 165mg/L, potassium iodide 0.82mg/L, boric acid 6.1mg/L, four water manganese sulfate 22.7mg/L, white vitriol
8.8mg/L, sodium molybdate 0.22mg/L, cupric sulfate pentahydrate 0.026mg/L, cobalt chloride 0.029mg/L, ferrous sulfate 28mg/L, second
Edetate disodium 24mg/L, inositol 98mg/L, niacin 0.25mg/L, puridoxine hydrochloride 0.3mg/L, thiamine hydrochloride
0.25mg/L, glycine 0.85mg/L.
4. a kind of preparation method of pleione bulbocodioides Initial culture base, it is characterised in that including,
The prewired step of solution：A great number of elements solution, trace element solution, iron salt solutions, organic component solution are prepared respectively；
Mixing step：A great number of elements solution, trace element solution, iron salt solutions, organic component solution, sucrose and agar powder are mixed
It closes, constant volume, sterilizing, cooling, solidification are to get pleione bulbocodioides Initial culture base.
5. preparation method as claimed in claim 4, which is characterized in that in the prewired step of solution, a great number of elements solution
Preparation method it is as follows：Potassium nitrate, ammonium nitrate, calcium chloride dihydrate, epsom salt, the potassium dihydrogen phosphate of formula ratio is taken to distinguish molten
It solves, then the containers store of potassium nitrate, ammonium nitrate, epsom salt, potassium dihydrogen phosphate mixing constant volume to 10L, calcium chloride dihydrate
The container of constant volume to 10L individually store, spare.
6. preparation method as claimed in claim 4, which is characterized in that in the prewired step of solution, the trace element solution
Preparation method it is as follows：Take the potassium iodide of formula ratio, boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate,
Cobalt chloride stores after dissolving respectively in mixing constant volume to the container of 5L, spare.
7. preparation method as claimed in claim 4, which is characterized in that in the prewired step of solution, the iron salt solutions are matched
Method processed is as follows：After taking the ferrous sulfate of formula ratio, disodium ethylene diamine tetraacetate to dissolve in mixing constant volume to the brown bottle of 5L, keep away
Light preserves, spare.
8. preparation method as claimed in claim 4, which is characterized in that in the prewired step of solution, the organic component solution
Preparation method it is as follows：Appearance of the constant volume to 5L after taking niacin, puridoxine hydrochloride, thiamine hydrochloride, the glycine of formula ratio to dissolve
It is stored for future use in device.
9. preparation method as claimed in claim 4, which is characterized in that the concrete operations of the mixing step are as follows：It measures respectively
A great number of elements solution, trace element solution, iron salt solutions, the organic component solution of formula ratio is taken to be added in the container of 1L, then
After the agar powder of 0.2-0.3g inositols, the sucrose of 2g/L, 4.8g/L are dissolved in mixing constant volume to the container of above-mentioned 1L, by container
It is heated to agar powder thawing, culture medium is dispensed into 30-40ml/ bottles of culture bottle, covers tightly bottle cap, culture bottle, which is put into temperature, is
121-125 DEG C, sterilize 20-30min under the sterilising conditions that pressure is 0.15-0.3MPa, sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap
It is put into culture medium storage room, waits for that its cooled and solidified is Initial culture base.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
| CN114223545A (en) * | 2022-01-12 | 2022-03-25 | 中国科学院昆明植物研究所 | Culture medium for improving germination rate of Pleione bulbocodioides distant hybrid seeds and application and cultivation method thereof |
| CN114431145A (en) * | 2022-02-15 | 2022-05-06 | 杭州市农业科学研究院 | Pleione tissue rapid propagation method based on somatic embryo approach |
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2017
- 2017-12-27 CN CN201711451055.4A patent/CN108353787A/en active Pending
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111072422A (en) * | 2020-01-16 | 2020-04-28 | 河南华薯农业科技有限公司 | Special culture medium for ipomoea batatas 32 and preparation method thereof |
| CN114223545A (en) * | 2022-01-12 | 2022-03-25 | 中国科学院昆明植物研究所 | Culture medium for improving germination rate of Pleione bulbocodioides distant hybrid seeds and application and cultivation method thereof |
| CN114431145A (en) * | 2022-02-15 | 2022-05-06 | 杭州市农业科学研究院 | Pleione tissue rapid propagation method based on somatic embryo approach |
| CN114431145B (en) * | 2022-02-15 | 2023-01-03 | 杭州市农业科学研究院 | Pleione tissue rapid propagation method based on somatic embryo approach |
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Effective date of registration: 20190410 Address after: 650000 6th Floor, Block A, Sunshine Building, 999 Kegao Road, Wuhua District, Kunming City, Yunnan Province Applicant after: Kangmei Pharmaceutical (Kunming) Germplasm Resources Co., Ltd. Address before: 663099 Wenshan Zhong and Miao Autonomous Prefecture Wenshan City, Yunnan Province, Kaihua South Road Wenshan Biovalley of Chinese Traditional Medicine 4-21 Applicant before: Kangmei Pharmaceutical (Wenshan) medicinal material planting Management Co., Ltd. |
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