CN108142291A - A kind of oldenlandia diffusa root media and preparation method thereof - Google Patents
A kind of oldenlandia diffusa root media and preparation method thereof Download PDFInfo
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- CN108142291A CN108142291A CN201711447174.2A CN201711447174A CN108142291A CN 108142291 A CN108142291 A CN 108142291A CN 201711447174 A CN201711447174 A CN 201711447174A CN 108142291 A CN108142291 A CN 108142291A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of oldenlandia diffusa root media, including a great number of elements component, micro- component, molysite component, organic constituents, plant hormone component, inositol, sucrose and agar powder；A great number of elements component includes：Potassium nitrate, ammonium nitrate, calcium chloride dihydrate, epsom salt and potassium dihydrogen phosphate；Trace element suite point includes：Potassium iodide, boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate and cobalt chloride；Molysite component includes：Ferrous sulfate and disodium ethylene diamine tetraacetate；Organic constituents include：Niacin, puridoxine hydrochloride, thiamine hydrochloride and glycine；Plant hormone component includes：Heteroauxin and 6 benzyladenines.Under appropriate conditions, oldenlandia diffusa proliferation seedling slowly grows flourishing root system, and flourishing root system can ensure the survival rate of oldenlandia diffusa transplanting.The invention also discloses a kind of preparation methods of oldenlandia diffusa root media, and flow is simple, easy to operate, and manufacturing cost is low.
Description
Technical field
It takes root training the present invention relates to oldenlandia diffusa tissue cultivating and seedling technical field more particularly to a kind of oldenlandia diffusa
Support base and preparation method thereof.
Background technology
Oldenlandia diffusa (Hedyotis diffusa Willd), which is that Rubiaceae is annual, hangs down loosely draft, high 15-50cm, root
It is elongated, branch, white flower.Stem is slightly rectangular or oblate cylindricality, Glabrous send out multi-branched from base portion, and in spring at florescence, kind is careful
Small yellowish-brown；It can receive for two seasons within 1 year, spring sowing August is received, and autumn planting is received November.The area of 800 meters of height above sea level is grown in, is grown in water more
Field, ridge and moistening spaciously.It is mainly distributed on the areas such as domestic Fujian, Guangdong, Hong Kong, Guangxi, Hainan, Anhui, Yunnan；
Its bitter, light, cold in nature, primary efficacy is clearing heat and detoxicating, dispersing swelling and dissipating binds, the diseases such as promoting urination and removing dampness, venomous snake bite, and discovered in recent years is white
Flower HERBA HEDYOTIS DIFFUSAE has preferable effect to cancer and hepatitis, and quantity is larger.
But oldenlandia diffusa is mostly wild, family's kind is seldom, and wild oldenlandia diffusa adulterant is more, there is corymbose hedyotis herb, fine flower
Auricled Hedyotis Herb, sealwort, sandwort, carpet weed, adulterant can be distinguished, but dry product and seed are difficult by tiny plant morphological characteristics when fresh
Distinguish the true and false.
And traditional Hedyotidis Diffusae breeding method is that oldenlandia grass seed is directly seeded into crop field, such sowing is deposited
In problems with：
(1) unevenness is broadcasted sowing, growing way is bad；
(2) it is influenced by temperature, humidity, the speed of growth is slow.
Invention content
For overcome the deficiencies in the prior art, one of the objects of the present invention is to provide a kind of oldenlandia diffusa culture of rootage
Base is 24-26 DEG C, intensity of illumination 2000-2500lx in temperature, and light application time is culture under conditions of 10-12 hours/day
15-25 days, oldenlandia diffusa proliferation seedling slowly grew flourishing root system, and flourishing root system can ensure oldenlandia diffusa transplanting
Survival rate.
The second object of the present invention is to provide a kind of preparation method of oldenlandia diffusa root media, and flow is simple,
Easy to operate, manufacturing cost is low.
An object of the present invention adopts the following technical scheme that realization：
A kind of oldenlandia diffusa root media, the culture medium include a great number of elements component, micro- component, molysite
Component, organic constituents, plant hormone component, inositol, sucrose and agar powder；The a great number of elements component includes：Potassium nitrate, nitre
Sour ammonium, calcium chloride dihydrate, epsom salt and potassium dihydrogen phosphate；The trace element suite point includes：Potassium iodide, boric acid, four water
Manganese sulfate, white vitriol, sodium molybdate, cupric sulfate pentahydrate and cobalt chloride；The molysite component includes：Ferrous sulfate and ethylenediamine
Tetraacethyl disodium；The organic constituents include：Niacin, puridoxine hydrochloride, thiamine hydrochloride and glycine；The plant hormone
Component includes：Heteroauxin and 6 benzyladenines.
Further, a concentration of 96-100mg/L of inositol, the mass percentage concentration of sucrose is 2%, the concentration of agar powder
For 4.8g/L.
Further, the concentration of each component is as follows in a great number of elements component：Potassium nitrate 1890-1900mg/L, nitric acid
Ammonium 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate 160-
170mg/L。
Further, the concentration of each component is as follows in the micro- component：Potassium iodide 0.8-0.83mg/L, boric acid
6.0-6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, white vitriol 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L,
Cupric sulfate pentahydrate 0.025-0.028mg/L, cobalt chloride 0.028-0.03mg/L.
Further, the concentration of each component is as follows in the molysite component：Ferrous sulfate 27.5-29mg/L, ethylenediamine tetraacetic
Acetic acid disodium 23-25mg/L.
Further, the concentration of each component is as follows in the organic constituents：Niacin 0.2-0.3mg/L, puridoxine hydrochloride
0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, glycine 0.8-0.9mg/L.
Further, the concentration of each component is as follows in the plant hormone component：Heteroauxin 1.6mg/L, 6 benzyl glands are fast
Purine 0.4mg/L.
Further, pH is adjusted to 5.8-6.0 by the culture medium with acid-base indicator.
The second object of the present invention adopts the following technical scheme that realization：
A kind of preparation method of oldenlandia diffusa root media, including：
A great number of elements mother liquor preparation process：Remaining a great number of elements component outside calcium chloride dihydrate is removed respectively, is configured to big
Secondary element mother liquor, the concentration of each component is 10-20 times of respective formula concentration in a great number of elements mother liquor；Calcium chloride dihydrate
It individually prepares, 10-20 times of a concentration of formula concentration of calcium chloride dihydrate solution；
Micro- mother liquor preparation process：Micro- component is taken respectively, is configured to micro- mother liquor, the micro member
The concentration of each component is 10 times of respective formula concentration in plain mother liquor；
Mother liquid of iron salt preparation process：Molysite component is taken respectively, is configured to mother liquid of iron salt, each component in the mother liquid of iron salt
Concentration is 10-20 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic constituents are taken respectively, are configured to organic matter mother liquor, in the organic matter mother liquor
The concentration of each component is 10-20 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Plant hormone mother liquor preparation process：Heteroauxin and 6 benzyladenines are taken respectively, is prepared with sterile water, are obtained dense
Degree is the heteroauxin mother liquor and 6 benzyladenine mother liquors of 1mg/ml；
Mix constant volume heating stepses：A great number of elements mother liquor 40-60ml, calcium chloride dihydrate solution 40-60ml, micro is taken respectively
Element mother liquor 20-30ml, mother liquid of iron salt 20-30ml, organic matter mother liquor 20-30ml, inositol solution, heteroauxin mother liquor 1.6ml,
6 benzyladenine mother liquor 0.4ml, agar powder 3.5-4.8g, sucrose 20-40g, mixing is calmly molten after sucrose dissolving to arrive 1L, then
Heating is then dispensed to culture bottle, after cooled and solidified until agar powder melts to obtain the final product.
Further, sterilization steps are further included：Heating is dispensed into culture bottle, every bottle of 30- until agar powder thawing
40ml covers tightly bottle cap, culture bottle is put into the autoclave that temperature is 121-125 DEG C, pressure is 0.15-0.3MPa, and sterilize 20-
30min, sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, after cooled and solidified to obtain the final product.
Compared with prior art, the beneficial effects of the present invention are：
(1) oldenlandia diffusa root media provided by the present invention, component are easy to get, and formula is reasonable, are oldenlandia diffusa
Suitable humiture, illumination condition are provided, sufficient nutritional ingredient increases light application time, can increase the speed of growth of plant,
Flourishing root system is differentiated, ensures the survival rate of plant；And it can ensure that oldenlandia diffusa does not connect in the tissue cultures stage of taking root
Touch external environment, it is ensured that kind is pure.Specifically, it is 24-26 DEG C, intensity of illumination 2000-2500lx in temperature, light application time
It under conditions of 10-12 hours/day, cultivates 15-25 days, oldenlandia diffusa proliferation seedling slowly grows flourishing root system, flourishing
Root system can ensure the survival rate of oldenlandia diffusa transplanting.
(2) preparation method of oldenlandia diffusa root media provided by the present invention, flow is simple, easy to operate, system
It is standby at low cost.
Specific embodiment
In the following, with reference to specific embodiment, the present invention is described further, it should be noted that is do not collided
Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.
A kind of oldenlandia diffusa root media, culture medium include a great number of elements component (potassium nitrate, ammonium nitrate, two water chlorine
Change calcium, epsom salt and potassium dihydrogen phosphate), micro- component (potassium iodide, boric acid, four water manganese sulfates, white vitriol,
Sodium molybdate, cupric sulfate pentahydrate and cobalt chloride), molysite component (ferrous sulfate and disodium ethylene diamine tetraacetate), organic constituents (cigarette
Acid, puridoxine hydrochloride, thiamine hydrochloride and glycine), plant hormone component (heteroauxin and 6 benzyladenines), inositol, sugarcane
Sugar and agar powder.
As preferred embodiment, the concentration of each component is as follows in a great number of elements component：Potassium nitrate 1890-1900mg/
L, ammonium nitrate 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, epsom salt 370-375mg/L, potassium dihydrogen phosphate
160-170mg/L；The concentration of each component is as follows in micro- component：Potassium iodide 0.8-0.83mg/L, boric acid 6.0-6.2mg/
L, four water manganese sulfate 22.3-23.0mg/L, white vitriol 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate
0.025-0.028mg/L, cobalt chloride 0.028-0.03mg/L；The concentration of each component is as follows in molysite component：Ferrous sulfate 27.5-
29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L；The concentration of each component is as follows in organic constituents：Niacin 0.2-0.3mg/
L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, glycine 0.8-0.9mg/L；Plant hormone group
The concentration of each component is as follows in point：Heteroauxin 1.6mg/L, 6 benzyladenine 0.4mg/L；A concentration of 96-100mg/ of inositol
L, the mass percentage concentration of sucrose is 2%, a concentration of 4.8g/L of agar powder.
As preferred embodiment, pH is adjusted to 5.8-6.0 by culture medium with acid-base indicator.
The oldenlandia diffusa root media that the embodiment of the present invention is provided, component are easy to get, and formula is reasonable, are oldenlandia
Grass provides suitable humiture, illumination condition, and sufficient nutritional ingredient increases light application time, can increase the growth speed of plant
Degree differentiates flourishing root system, ensures the survival rate of plant；And it can ensure oldenlandia diffusa not in the tissue cultures stage of taking root
Contact external environment, it is ensured that kind is pure.Specifically, it is 24-26 DEG C, intensity of illumination 2000-2500lx in temperature, during illumination
Between under conditions of 10-12 hour/day, to cultivate 15-25 days, oldenlandia diffusa proliferation seedling slowly grows the root system of prosperity, prosperity
Root system can ensure oldenlandia diffusa transplanting survival rate.
A kind of preparation method of oldenlandia diffusa root media, including：
A great number of elements mother liquor preparation process：Remaining a great number of elements component outside calcium chloride dihydrate is removed respectively, is configured to big
Secondary element mother liquor, the concentration of each component is 10-20 times of respective formula concentration in a great number of elements mother liquor；Calcium chloride dihydrate is independent
It prepares, 10-20 times of a concentration of formula concentration of calcium chloride dihydrate solution；
Micro- mother liquor preparation process：Micro- component is taken respectively, is configured to micro- mother liquor, and trace element is female
The concentration of each component is 10 times of respective formula concentration in liquid；
Mother liquid of iron salt preparation process：Molysite component is taken respectively, is configured to mother liquid of iron salt, the concentration of each component in mother liquid of iron salt
It is 10-20 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic constituents are taken respectively, are configured to organic matter mother liquor, each group in organic matter mother liquor
The concentration divided is 10-20 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Plant hormone mother liquor preparation process：Heteroauxin and 6 benzyladenines are taken respectively, is prepared with sterile water, are obtained dense
Degree is the heteroauxin mother liquor and 6 benzyladenine mother liquors of 1mg/ml；
Mix constant volume heating stepses：A great number of elements mother liquor 40-60ml, calcium chloride dihydrate solution 40-60ml, micro is taken respectively
Element mother liquor 20-30ml, mother liquid of iron salt 20-30ml, organic matter mother liquor 20-30ml, inositol solution, heteroauxin mother liquor 1.6ml,
6 benzyladenine mother liquor 0.4ml, agar powder 3.5-4.8g, sucrose 20-40g, mixing is calmly molten after sucrose dissolving to arrive 1L, then
Heating is then dispensed to culture bottle, after cooled and solidified until agar powder melts to obtain the final product.
As preferred embodiment, sterilization steps are further included：Heating is dispensed into until agar powder thawing in culture bottle,
Every bottle of 30-40ml, covers tightly bottle cap, culture bottle is put into the autoclave that temperature is 121-125 DEG C, pressure is 0.15-0.3MPa,
Sterilize 20-30min, and sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and is put into culture medium storage room, after cooled and solidified to obtain the final product.
As further embodiment, in a great number of elements mother liquor preparation process：Except calcium chloride dihydrate is individually matched
System storage is outer, remaining component mixed preparing in a great number of elements component.Because calcium chloride dihydrate mixes easily with other a great number of elements
Precipitation, individually storage can effectively avoid this problem.
As further embodiment, when preparing a great number of elements mother liquor, the dosage difference of each component is as follows：Potassium nitrate
370-390g, ammonium nitrate 320-340g, calcium chloride dihydrate 80-95g, epsom salt 70-75g, potassium dihydrogen phosphate 30-35g.
As further embodiment, when preparing micro- mother liquor, the dosage difference of each component is as follows：Potassium iodide
1.0-1.66g, boric acid 11.0-12.4g, four water manganese sulfate 40-46g, white vitriol 17.0-18.0g, sodium molybdate 0.2-
0.5g, cupric sulfate pentahydrate 0.05-0.06g, cobalt chloride 0.05-0.06g.
As further embodiment, when preparing mother liquid of iron salt, the dosage difference of each component is as follows：Ferrous sulfate
20-25g, disodium ethylene diamine tetraacetate 21-26g.
As further embodiment, when preparing organic matter mother liquor, the dosage difference of each component is as follows：Niacin 1.0-
1.5g, puridoxine hydrochloride 1.25-1.75g, thiamine hydrochloride 1.0-1.5g, glycine 4.0-4.5g.
As further embodiment, in inositol solution preparation process, 0.2-0.3g inositols are taken, it is sterile with 100ml
Water dissolution, obtains inositol solution, and inositol solution is now with the current.
The preparation method of oldenlandia diffusa root media that the embodiment of the present invention is provided, flow is simple, easy to operate,
Manufacturing cost is low.
It is specific embodiment of the present invention below, used raw material, equipment etc. remove special limit in the following embodiments
It can be obtained outside fixed by buying pattern.
Embodiment 1-5
A kind of oldenlandia diffusa root media, including a great number of elements component (potassium nitrate, ammonium nitrate, calcium chloride dihydrate, seven
Water magnesium sulfate and potassium dihydrogen phosphate), micro- component (potassium iodide, boric acid, four water manganese sulfates, white vitriol, sodium molybdate,
Cupric sulfate pentahydrate and cobalt chloride), molysite component (ferrous sulfate and disodium ethylene diamine tetraacetate), organic constituents (niacin, hydrochloric acid
Pyridoxol, thiamine hydrochloride and glycine), plant hormone component (heteroauxin and 6 benzyladenines), inositol, sucrose and fine jade
PH is adjusted to 5.8-6.0 by cosmetics, culture medium with acid-base indicator.Concentration of the above-mentioned each component in the root media such as following table
Shown in 1.
The formula table of the oldenlandia diffusa root media of 1 embodiment 1-5 of table
The oldenlandia diffusa root media of embodiment 1-5 is prepared in accordance with the following methods：
A great number of elements mother liquor preparation process：A great number of elements component is taken respectively, except calcium chloride dihydrate individually carries out preparation storage
Outside, remaining component mixed preparing in a great number of elements component obtains a great number of elements mother liquor, and each component is dense in a great number of elements mother liquor
Degree is 10 times of respective formula concentration；
Micro- mother liquor preparation process：Micro- component is taken respectively, is configured to micro- mother liquor, and trace element is female
The concentration of each component is 10 times of respective formula concentration in liquid；
Mother liquid of iron salt preparation process：Molysite component is taken respectively, is configured to mother liquid of iron salt, the concentration of each component in mother liquid of iron salt
It is 10 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic constituents are taken respectively, are configured to organic matter mother liquor, each group in organic matter mother liquor
The concentration divided is 10 times of respective formula concentration；
Inositol preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Plant hormone mother liquor preparation process：Heteroauxin and 6 benzyladenines are taken respectively, is prepared with sterile water, are obtained dense
Degree is the heteroauxin mother liquor and 6 benzyladenine mother liquors of 1mg/ml；
Mix constant volume heating stepses：Suitable a great number of elements mother liquor, micro- mother liquor, mother liquid of iron salt, organic is taken respectively
Object mother liquor, inositol solution, heteroauxin mother liquor, 6 benzyladenine mother liquors, agar powder and sucrose, mixing are fixed after sucrose dissolving
Molten to arrive 1L, then heating is until agar powder thawing, is dispensed into culture bottle, every bottle of 30-40ml covers tightly bottle cap, culture bottle is put
Into the autoclave that temperature is 123 DEG C, pressure is 0.25MPa, sterilize 25min, and sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle cap and puts
Room is stored to culture medium, after cooled and solidified to obtain the final product.
Effect assessment and performance detection
Purebred oldenlandia diffusa seedling is taken, traditional direct-seeding is taken to carry out nursery and using embodiment 1-5's respectively
Culture medium carries out nursery, and the condition of two kinds of training methods is：Temperature is 24-26 DEG C, intensity of illumination 2000-2500lx, light
It it is 10-12h/ days according to the time.Result of the test is recorded, it is as a result as shown in table 2 below.
2 oldenlandia grass seed of table carries out the logging table of tradition live streaming and culture medium tissue culture
It can be obtained from the record of table 2, oldenlandia diffusa seedling carries out tissue culture using the root media of embodiment 1-5,
The time for growing root system needs is significantly smaller than traditional direct-seeding seedling raising manners, and the half of only traditional direct sowing and seedling mode is left
It is right；And fruiting rate and implantation time are both significantly higher than traditional direct-seeding, and it is 0% that the adulterant of embodiment 1-5, which obscures rate, it is real
The root media for applying a 1-5 can effectively ensure that the pure of oldenlandia diffusa kind.
The above embodiment is only the preferred embodiment of the present invention, it is impossible to the scope of protection of the invention is limited with this,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (10)
1. a kind of oldenlandia diffusa root media, which is characterized in that the culture medium includes a great number of elements component, trace element
Component, molysite component, organic constituents, plant hormone component, inositol, sucrose and agar powder；The a great number of elements component includes：
Potassium nitrate, ammonium nitrate, calcium chloride dihydrate, epsom salt and potassium dihydrogen phosphate；The trace element suite point includes：Potassium iodide,
Boric acid, four water manganese sulfates, white vitriol, sodium molybdate, cupric sulfate pentahydrate and cobalt chloride；The molysite component includes：Sulfuric acid is sub-
Iron and disodium ethylene diamine tetraacetate；The organic constituents include：Niacin, puridoxine hydrochloride, thiamine hydrochloride and glycine；Institute
Plant hormone component is stated to include：Heteroauxin and 6 benzyladenines.
2. oldenlandia diffusa root media as described in claim 1, which is characterized in that a concentration of 96-100mg/ of inositol
L, the mass percentage concentration of sucrose is 2%, a concentration of 4.8g/L of agar powder.
3. oldenlandia diffusa root media as claimed in claim 2, which is characterized in that each group in a great number of elements component
The concentration divided is as follows：Potassium nitrate 1890-1900mg/L, ammonium nitrate 1640-1650mg/L, calcium chloride dihydrate 440-445mg/L, seven
Water magnesium sulfate 370-375mg/L, potassium dihydrogen phosphate 160-170mg/L.
4. oldenlandia diffusa root media as claimed in claim 3, which is characterized in that each group in the trace element component
The concentration divided is as follows：Potassium iodide 0.8-0.83mg/L, boric acid 6.0-6.2mg/L, four water manganese sulfate 22.3-23.0mg/L, seven water
Zinc sulfate 8.6-9.0mg/L, sodium molybdate 0.2-0.25mg/L, cupric sulfate pentahydrate 0.025-0.028mg/L, cobalt chloride 0.028-
0.03mg/L。
5. oldenlandia diffusa root media as claimed in claim 4, which is characterized in that each component in the molysite component
Concentration is as follows：Ferrous sulfate 27.5-29mg/L, disodium ethylene diamine tetraacetate 23-25mg/L.
6. oldenlandia diffusa root media as claimed in claim 5, which is characterized in that each component in the organic constituents
Concentration it is as follows：Niacin 0.2-0.3mg/L, puridoxine hydrochloride 0.25-0.35mg/L, thiamine hydrochloride 0.2-0.3mg/L, sweet ammonia
Sour 0.8-0.9mg/L.
7. oldenlandia diffusa root media as claimed in claim 6, which is characterized in that each group in the plant hormone component
The concentration divided is as follows：Heteroauxin 1.6mg/L, 6 benzyladenine 0.4mg/L.
8. oldenlandia diffusa root media as described in claim 1, which is characterized in that the culture medium acid-base indicator
PH is adjusted to 5.8-6.0.
9. a kind of preparation method of oldenlandia diffusa root media as described in claim 7 any one, which is characterized in that
Including：
A great number of elements mother liquor preparation process：Remaining a great number of elements component outside calcium chloride dihydrate is removed respectively, is configured to a large amount of members
Plain mother liquor, the concentration of each component is 10-20 times of respective formula concentration in a great number of elements mother liquor；Calcium chloride dihydrate is independent
It prepares, 10-20 times of a concentration of formula concentration of calcium chloride dihydrate solution；
Micro- mother liquor preparation process：Micro- component is taken respectively, is configured to micro- mother liquor, and the trace element is female
The concentration of each component is 10 times of respective formula concentration in liquid；
Mother liquid of iron salt preparation process：Molysite component is taken respectively, is configured to mother liquid of iron salt, the concentration of each component in the mother liquid of iron salt
It is 10-20 times of respective formula concentration；
Organic matter mother liquor preparation process：Organic constituents are taken respectively, are configured to organic matter mother liquor, each group in the organic matter mother liquor
The concentration divided is 10-20 times of respective formula concentration；
Inositol solution preparation process：Inositol is taken, with sterile water dissolution, obtains inositol solution；
Plant hormone mother liquor preparation process：Heteroauxin and 6 benzyladenines are taken respectively, is prepared with sterile water, and it is equal to obtain concentration
Heteroauxin mother liquor and 6 benzyladenine mother liquors for 1mg/ml；
Mix constant volume heating stepses：A great number of elements mother liquor 40-60ml, calcium chloride dihydrate solution 40-60ml, trace element are taken respectively
Mother liquor 20-30ml, mother liquid of iron salt 20-30ml, organic matter mother liquor 20-30ml, inositol solution, heteroauxin mother liquor 1.6ml, 6 benzyls
Base adenine mother liquor 0.4ml, agar powder 3.5-4.8g, sucrose 20-40g, mixing are fixed molten to 1L, Ran Houjia after sucrose dissolving
Heat is then dispensed to culture bottle, after cooled and solidified until agar powder melts to obtain the final product.
10. the preparation method of oldenlandia diffusa root media as claimed in claim 9, which is characterized in that further include sterilizing
Step：Heating is dispensed into culture bottle, every bottle of 30-40ml covers tightly bottle cap, and culture bottle is put into temperature until agar powder thawing
In the autoclave for being 0.15-0.3MPa for 121-125 DEG C, pressure, sterilize 20-30min, and sterilizing, which finishes, to take the dish out of the pot, and tightens culture bottle
It covers and is put into culture medium storage room, after cooled and solidified to obtain the final product.
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| CN105340739A (en) * | 2015-11-09 | 2016-02-24 | 广西壮族自治区药用植物园 | Oldenlandia diffusa tissue culture seedling rapid breeding method |
| CN106900554A (en) * | 2017-03-13 | 2017-06-30 | 玉林师范学院 | A kind of tissue culture and rapid propagation method of Chinese medicine Spatholobus sinensis chum et T. Chen |
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