CN110140473A - A kind of method of efficient water purification plant culture growth-promoting - Google Patents
A kind of method of efficient water purification plant culture growth-promoting Download PDFInfo
- Publication number
- CN110140473A CN110140473A CN201910448167.7A CN201910448167A CN110140473A CN 110140473 A CN110140473 A CN 110140473A CN 201910448167 A CN201910448167 A CN 201910448167A CN 110140473 A CN110140473 A CN 110140473A
- Authority
- CN
- China
- Prior art keywords
- promoting
- growth
- plant
- plant culture
- water purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The present invention relates to a kind of methods of efficient water purification plant culture growth-promoting, this method includes that the culture growth-promoting of vegetable seeds and the optimization of plant seedlings are cultivated, vegetable seeds growth-promoting cultivates material and uses graphene oxide and nano-titanium dioxide, seedling optimization culture liquid includes essential element, microelement and growth factor, wherein essential element includes graphene oxide, nanometer faujasite, growth factor includes the basic element of cell division, sodium humate, molasses separating liquid, the seed of plant early period and the bioactivity and vitality of plant can be improved in this method, enhance the plant later period in terms of sewage purification to organic pollutant, nitrogen, the utilization rate of Tiny ecosystem recycles between the degradation efficiency and plant root of phosphorus synergistic effect and carbon source, its sewage purification efficiency is much larger than common water plant.
Description
Technical field
The present invention relates to the fields of plant culture, are related to the culture growth-promoting of the plant to purify water, and in particular to Yi Zhonggao
The method for imitating water purification plant culture growth-promoting.
Background technique
As urbanization process constantly increases and urban population sharply increases, causes a series of municipal pollution and ask
Topic, so that the environmental carrying capacity of water body and ecology load power can't bear the heavy load in urban ecological environment, the ecosystem is seriously damaged.According to
Count park water BOD (BOD), the ammonia nitrogen (NH in 90% or more China3- N), the indexs such as total phosphorus (TP) severely exceed,
Eutrophication is extremely serious, causes Resources of Aquatic Plants gradually exhausted, the loss of biodiversity, the function and structure of water body
It is destroyed, aquatic ecosystem functional deterioration, wherein Freshwater ecosystems, which are disturbed, is particularly acute.The protection of aquatic ecosystem
Abundant attention by international community and the Chinese government, efficient water plant is as degeneration aquatic ecosystem restoration and reconstruction
Exploiting species, it has also become the hot spot of ecological study.External such as Germany, the U.S., state, Britain have published water plant monograph, in water
Plant Floristic Geography, ecology, group and vegetation and water plant and the relationship of environment etc. have done a large amount of work, state
Just begun with the work of comparison system after the interior eighties in water plant last century, but the diversity of water plant and protection,
Water plant denizen ecological invasion and bio-safety, degeneration aquatic vegetation reconstruction etc. research it is relatively fewer, with hair
It is compared up to country, either basis or application field, there are apparent gaps for domestic water plant research.With regard to water plant
For the application aspect of aquatic ecosystem restoration and reconstruction, there is water plant storage to absorb the nutrients such as N, P, purifying contaminated
Object promotes the functions such as other aquatiles metabolism, so that water plant has important application value.
Constantly increase now with urban water-body pollutant, especially nitrogen and phosphorus element content severely exceeds, and causes water body
Eutrophication it is very serious, say from ecological point, got in terms of sponge Patterns in Urban Wetland Park using the ratio of water plant
Come bigger, main function is for purifying sewage, achieving certain clean-up effect in different waters, but to nitrogen, phosphorus
It is seen on the absorption rate of element or efficiency is lower, and the death rate is higher during the growth process for plant, clears up not in time
It will result in the secondary pollution of water body.
Summary of the invention
In order to solve problem above, the present invention provides a kind of method of efficient water purification plant growth-promoting and cultivation, and this method can
The seed of plant early period and the bioactivity and vitality of plant are improved, enhances the plant later period in terms of sewage purification to having
Machine pollutant, nitrogen, phosphorus degradation efficiency and plant root between Tiny ecosystem recycle synergistic effect and carbon source utilization rate, sewage
Purification efficiency is much larger than common water plant, while emerges from the aesthetic values of landscape water body, constructs polluted water body
Ecological long-acting repair mechanism, keeps water quality well to stablize.
The present invention the following technical schemes are provided:
A kind of method of efficient water purification plant culture growth-promoting, method includes the following steps: vegetable seeds is promoted using seed
It is raw to cultivate material seed soaking growth-promoting, plant culture solution is added later, carries out the expansion culture of plant seedlings, wherein the training of seed growth-promoting
Educating material includes graphene oxide and nano-titanium dioxide；
This method is further comprising the steps of: being aerated clear water addition cultivating seeds device before vegetable seeds seed soaking growth-promoting
Processing, vegetable seeds is put into cultivating seeds device clear water and is impregnated, and adds cultivating seeds material seed soaking growth-promoting, plant training is added
The moisture of cultivating seeds device is removed before nutrient solution；
For the graphene oxide with a thickness of 0.6~1.0nm, the number of plies is 1~2 layer, the use of concentration is the molten of 4~6mg/mL
Liquid；
The nano-titanium dioxide is nanometer anatase titania of the partial size in 20~30nm, and titanium dioxide is as catalysis
Agent, generates light induced electron, its ultra-thin optical properties of material that graphene oxide provides passes through good translucency and heat transfer
Rate improves the conduction efficiency of electronics；
Plant culture solution include essential element graphene oxide, nanometer faujasite, sodium bicarbonate, potassium nitrate, ammonium chloride,
Potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, sodium chloride, sodium propionate；
Plant culture solution further include the growth factor basic element of cell division, sodium humate, molasses separating liquid, lysine, glycine,
Leucine, asparatate, glutamic acid, alanine, thiamine hydrochloride, niacin, multi-vitamins, sucrose, agar, inositol, ferment
Female extracting solution；
The partial size of the nanometer faujasite is 30~60nm；
The partial size of the basic element of cell division is 220~250nm, and the partial size of sodium humate is 5~8nm；
The molasses separating liquid is liquid of the molasses after centrifugation removal of impurities, 40~60 times is diluted after centrifugation, inositol is dilute
Release 100 times of liquid；
Plant culture solution further includes microelement iodate potassium, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulphate, chlorination
Cobalt, disodium ethylene diamine tetraacetate, ferrous sulfate.
The beneficial effects of the present invention are as follows:
1. the combination of both growth-promoting processing of the present invention to vegetable seeds, graphene oxide and titanium dioxide greatly reinforces
The temperature and photosynthetic efficiency in water plant seed and plant rhizosphere area promote vegetable seeds and the root system of water plant one
Growth and development under a opposite constant temperature, while seed coat cell activity is stimulated and enhancing, shorten water plant embryo
Bud scale, young stem, young root parenchyma cell between nutriment the unidirectional transportational process of short distance, adjust and improve significantly plant
The life cycle of object；
2. effectively increasing energy, the cell oxygen transmitting effect of cellular respiration generation present invention enhances the compatibility of biology
Can be active microbial flora biological respinse between root system, needed for the process for meeting plant cell active transport mineral element ion
The consumption wanted, it is advantageous regulate and control the dissolution oxygen balance and the distribution of microbiologic population in plant root region, it is easier to receive oxygen
The functional group on graphite alkene surface accelerates the foundation and balance of microecosystem in water body；
3. the present invention is to the processing of aquatic plant seed growth-promoting and optimizes the method cultivated, water plant root area is improved
Nutrient transformation efficiency in nutritive salt velocity of ion exchange and plant cell membrane or outside film, greatly strengthens plant nutritive salt
Active transport, metabolism ability, utilization of carbon source rate, water plant plant during the growth process plant cauline leaf growth rate and
To the absorption efficiency of nitrogen, phosphorus, while also improving remediation efficiency of the water plant in water environment treatment ecology chain.
Figure of description
Fig. 1 culture group and control group water plant germinating energy curve graph；
Fig. 2 culture group and control group water plant germination percentage curve graph；
Fig. 3 culture group and control group water plant seedling fresh weight growth chart；
Fig. 4 culture group and control group water plant vitality index curve graph.
Specific embodiment
Below by specific case study on implementation and Detailed description of the invention, the present invention is described in further detail, it should be understood that this
A little embodiments are merely to illustrate the present invention rather than limit the scope of the invention, after the present invention has been read, ability
It is as defined in the appended claims that field technique personnel fall within the application to the modification of various equivalent forms of the invention.
Embodiment 1
A kind of method of efficient water purification plant culture growth-promoting:
1) the culture growth-promoting of vegetable seeds
A1. preparing 3 diameters is the plastic tub of 37cm as cultivating seeds device, and the clear water for being separately added into 1L carries out 4h aeration
Processing is with spare；
B1. cyperus alternifolius, 3 groups of canna, Scirpus tabernaemontani vegetable seeds are chosen, selects 50 seeds to be bundled into pouch with gauze respectively,
It is respectively put into clear water in cultivating container after rinsing and impregnates 2h；
C1. prepare seed growth-promoting and cultivate material: taking graphene oxide solution 200mL that concentration is 5mg/mL and partial size is
The nano-titanium dioxide 40g of 25nm；
D1. soak seed growth-promoting: every group of cultivating seeds device is separately added into graphene oxide solution and nanometer anatase titania,
Seed soaking growth-promoting processing is done under outdoor natural conditions after mixing；
2) cultivation of plant seedlings
A2. plant seedlings optimization culture liquid is prepared:
Essential element: sodium bicarbonate (NaHCO3) 0.6g, potassium nitrate (KNO3) 0.5g, ammonium chloride (NH4Cl) 3.6g, phosphoric acid
Potassium dihydrogen (KH2PO4) 1.2g, dipotassium hydrogen phosphate (K2HPO4) 0.5g, magnesium sulfate (MgSO4·7H2O) 0.8g, calcium chloride
(CaCl2·2H2O) 0.8g, sodium chloride (NaCl2H2O) the nanometer eight of 0.4g, 5mg/mL graphene oxide solution 60ml, 50nm
Face zeolite 25g, sodium propionate 0.5g.
Microelement: potassium iodide (KI) 0.05g, boric acid (H3BO3) 0.005g, manganese sulfate (MnSO4·4H2O) 0.02g, sulphur
Sour zinc (ZnSO4·7H2O) 0.03g, sodium molybdate (Na2MoO4·2H2O) 0.01g, copper sulphate (CuSO4·5H2O) 0.001g, chlorine
Change cobalt (CoCl2·6H2O) 0.001g, disodium ethylene diamine tetraacetate (Na2EDTA) 0.5g, ferrous sulfate (FeSO4·7H2O)
0.02g。
Growth factor: nanometer phytochemicals (basic element of cell division) 10g, 6.5nm sodium humate 15g of 245nm, molasses separating liquid
12ml, lysine 0.015g, glycine 0.012g, leucine 0.01g, asparatate 0.012g, glutamic acid 0.01g, the third ammonia
Sour 0.01g, thiamine hydrochloride 0.015g, niacin 0.008g, multi-vitamins (B) 1.6g, sucrose 0.8g, agar 1.0g, inositol
60ml, yeast extract 30ml.
B2. it sops up the moisture of original seed growth-promoting cultivating container plastic tub respectively with siphonage, and adds respectively in culture dish
Enter culture solution, carries out the expansion culture of plant seedlings.
Embodiment 2
A kind of method of efficient water purification plant culture growth-promoting:
1) the culture growth-promoting of vegetable seeds
A1. preparing 3 diameters is the plastic tub of 37cm as cultivating seeds device, and the clear water for being separately added into 1L carries out 4h aeration
Processing is with spare；
B1. cyperus alternifolius, 3 groups of canna, Scirpus tabernaemontani vegetable seeds are chosen, selects 50 seeds to be bundled into pouch with gauze respectively,
It is respectively put into clear water in cultivating container after rinsing and impregnates 2h；
C1. prepare seed growth-promoting and cultivate material: taking graphene oxide solution 200mL that concentration is 4mg/mL and partial size is
The nano-titanium dioxide 40g of 20nm；
D1. soak seed growth-promoting: every group of cultivating seeds device is separately added into graphene oxide solution and nanometer anatase titania,
Seed soaking growth-promoting processing is done under outdoor natural conditions after mixing；
2) cultivation of plant seedlings
A2. plant seedlings optimization culture liquid is prepared:
Essential element: sodium bicarbonate (NaHCO3) 0.6g, potassium nitrate (KNO3) 0.5g, ammonium chloride (NH4Cl) 3.6g, phosphoric acid
Potassium dihydrogen (KH2PO4) 1.2g, dipotassium hydrogen phosphate (K2HPO4) 0.5g, magnesium sulfate (MgSO4·7H2O) 0.8g, calcium chloride
(CaCl2·2H2O) 0.8g, sodium chloride (NaCl2H2O) the nanometer eight of 0.4g, 4mg/mL graphene oxide solution 60ml, 30nm
Face zeolite 25g, sodium propionate 0.5g.
Microelement: potassium iodide (KI) 0.05g, boric acid (H3BO3) 0.005g, manganese sulfate (MnSO4·4H2O) 0.02g, sulphur
Sour zinc (ZnSO4·7 H2O) 0.03g, sodium molybdate (Na2MoO4·2H2O) 0.01g, copper sulphate (CuSO4·5H2O) 0.001g, chlorine
Change cobalt (CoCl2·6H2O) 0.001g, disodium ethylene diamine tetraacetate (Na2EDTA) 0.5g, ferrous sulfate (FeSO4·7H2O)
0.02g。
Growth factor: nanometer phytochemicals (basic element of cell division) 10g, 5nm sodium humate 15g of 220nm, molasses separating liquid
12ml, lysine 0.015g, glycine 0.012g, leucine 0.01g, asparatate 0.012g, glutamic acid 0.01g, the third ammonia
Sour 0.01g, thiamine hydrochloride 0.015g, niacin 0.008g, multi-vitamins (B) 1.6g, sucrose 0.8g, agar 1.0g, inositol
60ml, yeast extract 30ml.
B2. it sops up the moisture of original seed growth-promoting cultivating container plastic tub respectively with siphonage, and adds respectively in culture dish
Enter optimization culture liquid, carries out the expansion culture of plant seedlings.
Embodiment 3
A kind of method of efficient water purification plant culture growth-promoting:
1) the culture growth-promoting of vegetable seeds
A1. preparing 3 diameters is the plastic tub of 37cm as cultivating seeds device, and the clear water for being separately added into 1L carries out 4h aeration
Processing is with spare；
B1. cyperus alternifolius, 3 groups of canna, Scirpus tabernaemontani vegetable seeds are chosen, selects 50 seeds to be bundled into pouch with gauze respectively,
It is respectively put into clear water in cultivating container after rinsing and impregnates 2h；
C1. prepare seed growth-promoting and cultivate material: taking graphene oxide solution 200mL that concentration is 6mg/mL and partial size is
The nano-titanium dioxide 40g of 30nm；
D1. soak seed growth-promoting: every group of cultivating seeds device is separately added into graphene oxide solution and nanometer anatase titania,
Seed soaking growth-promoting processing is done under outdoor natural conditions after mixing；
2) cultivation of plant seedlings
A2. plant seedlings optimization culture liquid is prepared:
Essential element: sodium bicarbonate (NaHCO3) 0.6g, potassium nitrate (KNO3) 0.5g, ammonium chloride (NH4Cl) 3.6g, phosphoric acid
Potassium dihydrogen (KH2PO4) 1.2g, dipotassium hydrogen phosphate (K2HPO4) 0.5g, magnesium sulfate (MgSO4·7H2O) 0.8g, calcium chloride
(CaCl2·2H2O) 0.8g, sodium chloride (NaCl2H2O) the nanometer eight of 0.4g, 6mg/mL graphene oxide solution 60ml, 40nm
Face zeolite 25g, sodium propionate 0.5g.
Microelement: potassium iodide (KI) 0.05g, boric acid (H3BO3) 0.005g, manganese sulfate (MnSO4·4H2O) 0.02g, sulphur
Sour zinc (ZnSO4·7 H2O) 0.03g, sodium molybdate (Na2MoO4·2H2O) 0.01g, copper sulphate (CuSO4·5H2O) 0.001g, chlorine
Change cobalt (CoCl2·6H2O) 0.001g, disodium ethylene diamine tetraacetate (Na2EDTA) 0.5g, ferrous sulfate (FeSO4·7H2O)
0.02g。
Growth factor: nanometer phytochemicals (basic element of cell division) 10g, 7nm sodium humate 15g of 240nm, molasses separating liquid
12ml, lysine 0.015g, glycine 0.012g, leucine 0.01g, asparatate 0.012g, glutamic acid 0.01g, the third ammonia
Sour 0.01g, thiamine hydrochloride 0.015g, niacin 0.008g, multi-vitamins (B) 1.6g, sucrose 0.8g, agar 1.0g, inositol
60ml, yeast extract 30ml.
B2. it sops up the moisture of original seed growth-promoting cultivating container plastic tub respectively with siphonage, and adds respectively in culture dish
Enter optimization culture liquid, carries out the expansion culture of plant seedlings.
Embodiment 4
A kind of method of efficient water purification plant culture growth-promoting:
1) the culture growth-promoting of vegetable seeds
A1. preparing 3 diameters is the plastic tub of 37cm as cultivating seeds device, and the clear water for being separately added into 1L carries out 4h aeration
Processing is with spare；
B1. cyperus alternifolius, 3 groups of canna, Scirpus tabernaemontani vegetable seeds are chosen, selects 50 seeds to be bundled into pouch with gauze respectively,
It is respectively put into clear water in cultivating container after rinsing and impregnates 2h；
C1. prepare seed growth-promoting and cultivate material: taking graphene oxide solution 200mL that concentration is 5mg/mL and partial size is
The nano-titanium dioxide 40g of 25nm；
D1. soak seed growth-promoting: every group of cultivating seeds device is separately added into graphene oxide solution and nanometer anatase titania,
Seed soaking growth-promoting processing is done under outdoor natural conditions after mixing；
2) cultivation of plant seedlings
A2. plant seedlings optimization culture liquid is prepared:
Essential element: sodium bicarbonate (NaHCO3) 0.6g, potassium nitrate (KNO3) 0.5g, ammonium chloride (NH4Cl) 3.6g, phosphoric acid
Potassium dihydrogen (KH2PO4) 1.2g, dipotassium hydrogen phosphate (K2HPO4) 0.5g, magnesium sulfate (MgSO4·7H2O) 0.8g, calcium chloride
(CaCl2·2H2O) 0.8g, sodium chloride (NaCl2H2O) the nanometer eight of 0.4g, 5mg/mL graphene oxide solution 60ml, 60nm
Face zeolite 25g, sodium propionate 0.5g.
Microelement: potassium iodide (KI) 0.05g, boric acid (H3BO3) 0.005g, manganese sulfate (MnSO4·4H2O) 0.02g, sulphur
Sour zinc (ZnSO4·7 H2O) 0.03g, sodium molybdate (Na2MoO4·2H2O) 0.01g, copper sulphate (CuSO4·5H2O) 0.001g, chlorine
Change cobalt (CoCl2·6H2O) 0.001g, disodium ethylene diamine tetraacetate (Na2EDTA) 0.5g, ferrous sulfate (FeSO4·7H2O)
0.02g。
Growth factor: nanometer phytochemicals (basic element of cell division) 10g, 8nm sodium humate 15g of 250nm, molasses separating liquid
12ml, lysine 0.015g, glycine 0.012g, leucine 0.01g, asparatate 0.012g, glutamic acid 0.01g, the third ammonia
Sour 0.01g, thiamine hydrochloride 0.015g, niacin 0.008g, multi-vitamins (B) 1.6g, sucrose 0.8g, agar 1.0g, inositol
60ml, yeast extract 30ml.
B2. it sops up the moisture of original seed growth-promoting cultivating container plastic tub respectively with siphonage, and adds respectively in culture dish
Enter optimization culture liquid, carries out the expansion culture of plant seedlings.
The technical effect of technical solution of the present invention is embodied below by way of comparative experiments and relevant experimental data:
(1) the cultivate effect comparative experiments of water plant seed
1) chooses three groups of plants of embodiment 1, as processing growth-promoting cultivation group (EG) (cyperus alternifolius EG, canna EG, water
Green onion EG)；
2) still further three groups of untreated control groups (CK) of choosing group (cyperus alternifolius CK, canna CK, Scirpus tabernaemontani CK) are handled simultaneously:
The vegetable seeds for taking cyperus alternifolius, canna, Scirpus tabernaemontani takes the plastic tub of 3 diameter 37cm as cultivating seeds device, takes the clear water of 3L
And it is spare by the Air Exposure of 4h, every kind of plant selects 50 seeds to be bundled into pouch, three groups of vegetable seeds drifts with gauze respectively
It is respectively put into after washing in the clear water of 1L and soaks 2h, directly do seed soaking growth-promoting processing under outdoor natural conditions later.
3) fills up labelled, dated sample number, kind, number after moisture in the culture vessel of 6 groups of vegetable seeds
Amount is cultivated Start Date, and the germination quantity of the 4th day measurement seed calculates germinating energy；
Record the germinative number of seed day by day from the 4th day, the germination percentage and seedling fresh weight of the 10th day measurement seed calculate kind
Sub- vitality index (SVI), Seed Integrated vigor Index are the concentrated expression of germination rate and increment, are the measurements of seed vitality
Standard；
Germinating energy (%)=(normal chitting piece number/cultivation seed number in 4d) × 100
Germination percentage (%)=(normal chitting piece number/cultivation seed number in 10d) × 100
SV=germination percentage (%) × seedling fresh weight (g).
4) water plant seed Biological control result:
Fig. 1 to Fig. 4 be water plant seed growth-promoting development experiment datagram, chart data be respectively seedling fresh weight,
Four germinating energy, germination percentage, vitality index indicatrixs, can be seen that by experimental data figure
1. handling the germinating energy of growth-promoting cultivation group (EG) vegetable seeds and the ratio of untreated control group (CK) are as follows: cyperus alternifolius
EG germinating energy is 1.31 times of cyperus alternifolius CK germinating energy；Canna EG germinating energy is 1.25 times of canna CK germinating energy；Scirpus tabernaemontani
EG germinating energy is 1.22 times of Scirpus tabernaemontani CK germinating energy；
2. handling the germination percentage of growth-promoting cultivation group (EG) vegetable seeds and the ratio of untreated control group (CK) are as follows: cyperus alternifolius
EG germination percentage is 1.5 times of cyperus alternifolius CK germination percentage；Canna EG germination percentage is 1.44 times of canna CK germination percentage；Scirpus tabernaemontani EG
Germination percentage is 1.49 times of Scirpus tabernaemontani CK germination percentage；
3. handling the seedling fresh weight of growth-promoting cultivation group (EG) vegetable seeds and the ratio of untreated control group (CK) are as follows: windmill
Careless EG seedling fresh weight is 1.69 times of cyperus alternifolius CK seedling fresh weight；Canna EG seedling fresh weight is canna CK seedling fresh weight
2.00 again；Scirpus tabernaemontani EG seedling fresh weight is 2.14 times of Scirpus tabernaemontani CK seedling fresh weight；
4. handling the vitality index of growth-promoting cultivation group (EG) vegetable seeds and the ratio of untreated control group (CK) are as follows: windmill
Careless EG vitality index is 3.26 times of cyperus alternifolius CK vitality index；Canna EG vitality index is canna CK vitality index
3.43 again；Scirpus tabernaemontani EG vitality index is 3.32 times of Scirpus tabernaemontani CK vitality index.
(2) the cultivate effect comparative experiments of water plant seedling
1) prepares optimization culture liquid, the optimization culture liquid (OM) for selecting embodiment 1 to prepare；
2) prepares control group, prepares Nostoc commune Vanch liquid (MS): a great number of elements: ammonium nitrate (NH4NO3) 1.65g, potassium nitrate
(KNO3) 1.9g, calcium chloride (CaCl2·2H2O) 0.44g, magnesium sulfate (MgSO4·7H2O) 0.37g, potassium dihydrogen phosphate (KH2PO4)
0.17g；
Microelement: potassium iodide (KI) 0.83mg, boric acid (H3BO3) 6.2mg, manganese sulfate (MnSO4·4H2O)22.3mg、
Zinc sulfate (ZnSO4·7H2O) 8.6mg, molybdic acid receive (Na2MoO4·2H2O) 0.25mg, copper sulphate (CuSO4·5H2O)
0.025mg, cobalt chloride (CoCl2·6H2O)0.025mg；
Molysite: green vitriol (FeSO4·7H2O) 27.8mg, disodium ethylene diamine tetraacetate (Na2-EDTA·
2H2O)37.3mg；
Organic substance: thiamine hydrochloride 0.1mg, puridoxine hydrochloride 0.5mg, niacin 0.5mg, sucrose 30g, agar 7g, flesh
Alcohol 100mg, pantothenic acid 0.5mg, glycine 2.0mg；
3) choose the cyperus alternifolius of a height of 8cm, canna, Scirpus tabernaemontani seed growth-promoting young shoot be to cultivate object, 3 excellent
The plant seedlings basin for changing culture solution (OM) culture is culture group (EG) (cyperus alternifolius EG, canna EG, Scirpus tabernaemontani EG), and 3 common
The plant seedlings basin of culture solution culture (MS) is control group (CK) (cyperus alternifolius CK, canna CK, Scirpus tabernaemontani CK), is distinguished with siphonage
The moisture for sopping up original seed growth-promoting cultivating container plastic tub is separately added into optimization (OM) culture solution in the vessel of culture group EG,
The expansion cultivation that (MS) culture solution carries out plant seedlings is added in control group CK, then the labelled, note in each plastic tub
Bright sample number kind, quantity, is cultivated Start Date, the survival rate of the 15th day measurement seedling plants, is remembered daily in incubation
Temperature, humidity are recorded, is supplemented in plastic tub with distilled water due to plant absorption and the moisture evaporated.
Growth survival rate of the 1 plant seedlings plant of table under different condition of culture
It can be seen that the plant plumule of growth-promoting uses the result of different culture solutions progress seedling culture by experimental data are as follows: plant
The growth survival and plant seedlings (MS) culture solution survival rate ratio of object seedling optimization (OM) culture solution are as follows: cyperus alternifolius optimization
(OM) growth survival of culture solution is 1.67 times of plant seedlings (MS) culture solution；The growth of canna optimization (OM) culture solution
Survival rate is 1.75 times of plant seedlings (MS) culture solution；The growth survival that Scirpus tabernaemontani optimizes (OM) culture solution is plant seedlings
(MS) 1.52 times of culture solution.
(3) water plant purifying water effect comparative experiments
1) choose robust growth, growing way uniformly, without withered and yellow leaf, highly be 30cm plant, 3 groups for optimization training
The plant grown in nutrient solution is experimental group (EG) (cyperus alternifolius EG, canna EG, Scirpus tabernaemontani EG), and 3 groups are raw in Nostoc commune Vanch liquid
Long plant is control group (CK) (cyperus alternifolius CK, canna CK, Scirpus tabernaemontani CK), in the cultivation plastic tub of 6 groups of water plant seedling
In be separately added into 0.005kg agar, gel strength is 1800 degree, ratio 1:300；
2) it is the plastic cylinder of 20cm*35cm that, which selects diameter * height, cultivates base in the plant shoots that drum bottom is laid with
Material is the faujasite of partial size 0.5cm, and laying depth 8cm takes root for water plant；
3) simulates the water quality of sewage: initial ammonia nitrogen concentration is 20.5mg/L, and total phosphorus concentration 4.9mg/L, COD concentration is
120mg/L, sewage quantity are 4L/ barrels, need to test sewage about 24L altogether, have an area of 3~5 cm ranges in plant root and connect root screening, move
Enter progress sewage purification experiment in plastic cylinder；
4) is labelled after plastic cylinder fills up sewage, indicates sample number, kind, quantity, water purification experiment beginning day
Phase records temperature, humidity daily during experiment, in distilled water supplement drum due to plant absorption and the moisture that evaporates, the
Plant performance, drop data under pollution index are measured within 20 days in practical applications；
Specific measurement item: 1. absorptivity of the plant plant cauline leaf to nitrogen phosphorus；2. to the removal rate of nitrogen phosphorus in sewage；3. planting
Object plant strain growth rate；4. biomass growth rate；5. root system of plant region DO situation of change；6. rhizosphere microbial growing state；⑦
Indices of the root system of plant microorganism to utilization of carbon source situation.
The absorptivity of 2 culture group of table and control group plants cauline leaf to nitrogen
The absorptivity of 3 culture group of table and control group plants cauline leaf to phosphorus
The ammonia nitrogen removal frank of table 4 culture group and control group plants
Total tp removal rate of table 5 culture group and control group plants
It can be seen that by experimental data
1. the result of experimental group (EG) and control group (CK) cauline leaf in terms of sewage nitrogen, phosphorus absorptivity are as follows:
For cyperus alternifolius EG to 9.85 times that the absorptivity of ammonia nitrogen is cyperus alternifolius CK, canna EG is beauty to the absorptivity of ammonia nitrogen
8.13 times of any of several broadleaf plants CK, Scirpus tabernaemontani EG is to 2.0 times that the absorptivity of ammonia nitrogen is Scirpus tabernaemontani CK；
For cyperus alternifolius EG to 4.72 times that the absorptivity of phosphorus is cyperus alternifolius CK, canna EG is canna CK to the absorptivity of phosphorus
3.78 times, 2.13 times to the absorptivity Scirpus tabernaemontani CK of phosphorus of Scirpus tabernaemontani EG.
2. the result of experimental group (EG) and control group (CK) plant in terms of sewage nitrogen, total tp removal rate are as follows:
For cyperus alternifolius EG to 2.91 times of the removal rate cyperus alternifolius CK of ammonia nitrogen, canna EG is canna to the removal rate of ammonia nitrogen
2.77 times of CK, Scirpus tabernaemontani EG is to 3.08 times that the removal rate of ammonia nitrogen is Scirpus tabernaemontani CK；
For cyperus alternifolius EG to 2.42 times that the removal rate of total phosphorus is cyperus alternifolius CK, canna EG is beauty to the removal rate of total phosphorus
2.41 times of any of several broadleaf plants CK, Scirpus tabernaemontani EG is to 2.33 times that the removal rate of total phosphorus is Scirpus tabernaemontani CK.
6 culture group of table and control group plants growth rate
The result of experimental group (EG) and control group (CK) plant in terms of growth rate are as follows: cyperus alternifolius EG plant height is
2.41 times of cyperus alternifolius CK plant height, canna EG plant height are 2.28 times of canna CK plant height, and Scirpus tabernaemontani EG plants
Plant height degree is 2.20 times of Scirpus tabernaemontani CK plant height.
7 culture group of table and control group plants biomass growth rate
The result of experimental group (EG) and control group (CK) in terms of plant biomass growth rate are as follows: cyperus alternifolius EG plant
Biomass is 3.58 times of cyperus alternifolius CK biomass, and canna EG biomass is canna CK biomass
2.72 times, Scirpus tabernaemontani EG biomass is 6.03 times of Scirpus tabernaemontani CK biomass.
8 difference HRT culture group of table and control group plants root area DO change mg/L
The result of experimental group (EG) and control group (CK) in terms of root area dissolved oxygen (DO) variation are as follows: cyperus alternifolius EG's
Root system of plant region soluble oxygen amount is 2.41 times of cyperus alternifolius CK, and the root system of plant region soluble oxygen amount of canna EG is canna
2.46 times of CK, the root system of plant region soluble oxygen amount of Scirpus tabernaemontani EG are 2.40 times of Scirpus tabernaemontani CK；
Dissolved oxygen result of variations proves, water plant be to the dissolved oxygen for improving rhizosphere area solution it is beneficial, in rhizosphere layer
Residence time is short and control group (CK) solution in dissolved oxygen content it is higher instead, this phenomenon reflects, is passed by water plant
The dissolved oxygen in rhizosphere area is transported to, aerobic microbiological mass propagation is supplied, to the conversion degraded with oxidation of organic compounds and oxygen, is promoted
The procreation of facultative microbe, the DO concentration of rhizosphere area aqueous solution are maintained between 2.0~3.0mg/L between root system, microbial bacteria
Group's distribution situation is good.
Utilization rate of the root system of plant microorganism to carbon source (COD) under the different condition of culture of table 9
In the bigger serface that same faujasite matrix provides, experimental group (EG) and control group (CK) are being planted
Object rhizosphere microbial is to result in utilization of carbon source rate are as follows: the root system of plant Microbial source utilization rate of cyperus alternifolius EG is cyperus alternifolius CK
1.59 times, the root system of plant microorganism of canna EG is 1.85 times of canna CK, the plant of Scirpus tabernaemontani EG to utilization of carbon source rate
Rhizosphere microbial is to 2.07 times that utilization of carbon source rate is Scirpus tabernaemontani CK, it can be seen that vegetable seeds is after growth-promoting and optimization culture
The microbial proliferation performance in water plant rhizosphere region can be enhanced in plant, is also greatly improved water plant root system to carbon
Utilization rate, while COD content in Synergistic degradation sewage.
10 culture group of table and control group plants rhizosphere microbial growth change cfu/mL
The result of experimental group (EG) and control group (CK) in terms of root system of plant microorganism growth rate are as follows: the plant of cyperus alternifolius EG
Object rhizosphere microbial growth rate is 98.5 times of cyperus alternifolius CK, and the root system of plant microorganism growth rate of canna EG is cyperus alternifolius CK
102.7 times, the root system of plant microorganism growth rate of Scirpus tabernaemontani EG is 64.9 times of cyperus alternifolius CK；
Rhizosphere microbial growth rate shows that the absorption of root system of plant and ion exchange are strong, on the section of root system, by
It is outer and in form three reaction zones of aerobic, amphimicrobian and anaerobism, microorganism is after domestication after a period of time, different bacterium
Group occupies different reaction zones, promotes the rhizosphere microbial flora of water plant normal in a metastable carbon cycle
Growth and procreation, root system of plant form natural biomembrane after adsorbing beneficial microbe colony, and dissolved organic matter then passes through plant
Absorption, absorption and the biomineralization process of object root system biomembrane and the removal that is decomposed, while microorganism can also give aquatic insect
Bait is provided, fish can ingest insect again, to form a small-sized microecological balance system between root system of plant.
Claims (10)
1. a kind of method of efficient water purification plant culture growth-promoting, which is characterized in that method includes the following steps: vegetable seeds makes
Material seed soaking growth-promoting is cultivated with seed growth-promoting, plant culture solution is added later, carries out the expansion culture of plant seedlings, wherein kind
It includes graphene oxide and nano-titanium dioxide that sub- growth-promoting, which cultivates material,.
2. by the method for efficient water purification plant culture growth-promoting described in claim 1, which is characterized in that this method further includes following
Step: cultivating seeds device is added in clear water before vegetable seeds seed soaking growth-promoting and carries out Air Exposure, then vegetable seeds is put into kind
Sub- cultivating container seed soaking growth-promoting, removes the moisture of cultivating seeds device before plant culture solution is added.
3. by the method for efficient water purification plant culture growth-promoting described in claim 1, which is characterized in that the graphene oxide is thick
Degree is 0.6 ~ 1.0nm, and the number of plies is 1 ~ 2 layer, the solution for the use of concentration being 4 ~ 6mg/mL.
4. by the method for efficient water purification plant culture growth-promoting described in claim 1, which is characterized in that the nano-titanium dioxide
For partial size 20 ~ 30nm nanometer anatase titania.
5. the method for efficient water purification plant culture growth-promoting as described in claim 1 or 3, which is characterized in that plant culture solution packet
Include essential element graphene oxide, nanometer faujasite, sodium bicarbonate, potassium nitrate, ammonium chloride, potassium dihydrogen phosphate, phosphoric acid hydrogen two
Potassium, magnesium sulfate, calcium chloride, sodium chloride, sodium propionate.
6. by the method for efficient water purification plant culture growth-promoting described in claim 1, which is characterized in that plant culture solution further includes
The growth factor basic element of cell division, sodium humate, molasses separating liquid, lysine, glycine, leucine, asparatate, paddy ammonia
Acid, alanine, thiamine hydrochloride, niacin, multi-vitamins, sucrose, agar, inositol, yeast extract.
7. the method for efficient water purification plant culture growth-promoting as described in claim 5, which is characterized in that the nanometer faujasite
Partial size be 30 ~ 60nm.
8. by the method for efficient water purification plant culture growth-promoting as claimed in claim 6, which is characterized in that the basic element of cell division
Partial size is 220 ~ 250nm, and the partial size of sodium humate is 5 ~ 8nm.
9. by the method for efficient water purification plant culture growth-promoting as claimed in claim 6, which is characterized in that the molasses separating liquid is
Liquid of the molasses after centrifugation removal of impurities dilutes 40 ~ 60 times after centrifugation, inositol is the liquid for diluting 100 times.
10. by the method for efficient water purification plant culture growth-promoting described in claim 1, which is characterized in that plant culture solution also wraps
Include microelement iodate potassium, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulphate, cobalt chloride, disodium ethylene diamine tetraacetate, sulphur
It is sour ferrous.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910448167.7A CN110140473B (en) | 2019-05-27 | 2019-05-27 | Method for culturing and promoting growth of high-efficiency water-purifying plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910448167.7A CN110140473B (en) | 2019-05-27 | 2019-05-27 | Method for culturing and promoting growth of high-efficiency water-purifying plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110140473A true CN110140473A (en) | 2019-08-20 |
| CN110140473B CN110140473B (en) | 2022-03-22 |
Family
ID=67593379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910448167.7A Active CN110140473B (en) | 2019-05-27 | 2019-05-27 | Method for culturing and promoting growth of high-efficiency water-purifying plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110140473B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158366A (en) * | 2021-11-10 | 2022-03-11 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for cutting propagation of chenopodium camellinum |
| CN115088602A (en) * | 2022-06-29 | 2022-09-23 | 新疆兵团勘测设计院(集团)有限责任公司 | Cultivation method for improving saline-alkali resistance of plants and ecological system construction method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107750892A (en) * | 2017-10-23 | 2018-03-06 | 柯江波 | Graphene is modified indoor hydroponic plant nutrient solution and preparation method thereof |
| CN108157081A (en) * | 2017-12-28 | 2018-06-15 | 塔里木大学 | A kind of cultural method of fennel |
| CN108157085A (en) * | 2017-12-28 | 2018-06-15 | 塔里木大学 | A kind of implantation methods of winter wheat |
| CN108752099A (en) * | 2018-06-19 | 2018-11-06 | 江苏东珠景观股份有限公司 | Conducive to the water plant culture solution and preparation method thereof that purifies water of ecological circulation |
| CN108935517A (en) * | 2018-07-11 | 2018-12-07 | 广西壮族自治区药用植物园 | Promote promotor of Guangxi Folium hydrangeae strigosae germination and preparation method thereof and its application |
| CN109121535A (en) * | 2018-07-25 | 2019-01-04 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method of cucumber seeds |
| CN109220044A (en) * | 2018-07-25 | 2019-01-18 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of implantation methods promoting cucumber economic benefit |
| CN109293131A (en) * | 2018-09-18 | 2019-02-01 | 广州微问环保科技有限公司 | A kind of method of original position self-purification black and odorous water |
-
2019
- 2019-05-27 CN CN201910448167.7A patent/CN110140473B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107750892A (en) * | 2017-10-23 | 2018-03-06 | 柯江波 | Graphene is modified indoor hydroponic plant nutrient solution and preparation method thereof |
| CN108157081A (en) * | 2017-12-28 | 2018-06-15 | 塔里木大学 | A kind of cultural method of fennel |
| CN108157085A (en) * | 2017-12-28 | 2018-06-15 | 塔里木大学 | A kind of implantation methods of winter wheat |
| CN108752099A (en) * | 2018-06-19 | 2018-11-06 | 江苏东珠景观股份有限公司 | Conducive to the water plant culture solution and preparation method thereof that purifies water of ecological circulation |
| CN108935517A (en) * | 2018-07-11 | 2018-12-07 | 广西壮族自治区药用植物园 | Promote promotor of Guangxi Folium hydrangeae strigosae germination and preparation method thereof and its application |
| CN109121535A (en) * | 2018-07-25 | 2019-01-04 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method of cucumber seeds |
| CN109220044A (en) * | 2018-07-25 | 2019-01-18 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of implantation methods promoting cucumber economic benefit |
| CN109293131A (en) * | 2018-09-18 | 2019-02-01 | 广州微问环保科技有限公司 | A kind of method of original position self-purification black and odorous water |
Non-Patent Citations (6)
| Title |
|---|
| 乔俊等: "纳米炭材料对作物生长影响的研究进展 ", 《农业工程学报》 * |
| 吴金海等: "氧化石墨烯处理对甘蓝型油菜生长发育的影响 ", 《基因组学与应用生物学》 * |
| 文双喜 等: "水培实验中不同粒径纳米TiO2对芦苇种子发芽和植株生长和生理的影响", 《生态毒理学报》 * |
| 汪玉洁 等: "纳米材料在农业上的应用及其对植物生长和发育的影响", 《植物生理学报》 * |
| 王晓静 等: "氧化石墨烯拌种对高羊茅种子萌发与幼苗生长的影响", 《种子》 * |
| 赵振杰 等: "碳纳米材料对植物生长发育的调节作用", 《作物杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158366A (en) * | 2021-11-10 | 2022-03-11 | 内蒙古蒙草生态环境（集团）股份有限公司 | Method for cutting propagation of chenopodium camellinum |
| CN115088602A (en) * | 2022-06-29 | 2022-09-23 | 新疆兵团勘测设计院(集团)有限责任公司 | Cultivation method for improving saline-alkali resistance of plants and ecological system construction method thereof |
| CN115088602B (en) * | 2022-06-29 | 2023-08-18 | 新疆兵团勘测设计院集团股份有限公司 | Cultivation method for improving salt and alkali resistance of plants and ecological system construction method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110140473B (en) | 2022-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ji et al. | New insights for enhancing the performance of constructed wetlands at low temperatures | |
| Li et al. | Microalgal and duckweed based constructed wetlands for swine wastewater treatment: A review | |
| Hu et al. | Influence of exogenous CO 2 on biomass and lipid accumulation of microalgae Auxenochlorella protothecoides cultivated in concentrated municipal wastewater | |
| Yang et al. | Cultivation of oleaginous microalgae for removal of nutrients and heavy metals from biogas digestates | |
| Kaloudas et al. | Phycoremediation of wastewater by microalgae: a review | |
| CN101633894B (en) | Culture medium of euglena gracilis and open type high-density culture method | |
| Shahi Khalaf Ansar et al. | Removal of organic and inorganic contaminants from the air, soil, and water by algae | |
| CN102747014B (en) | High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment | |
| CN113322209B (en) | Plant growth-promoting composite microbial inoculum produced based on ecological relationship among strains and application thereof | |
| CN109486699A (en) | One plant of salt tolerant aerobic denitrifying bacteria and application | |
| CN109370954A (en) | It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method | |
| CN110140473A (en) | A kind of method of efficient water purification plant culture growth-promoting | |
| CN101805716A (en) | Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof | |
| Sun et al. | A review on algal-bacterial symbiosis system for aquaculture tail water treatment | |
| Yu et al. | Ecological responses of three emergent aquatic plants to eutrophic water in Shanghai, PR China | |
| CN104560787B (en) | A kind of peanut plant growth-promoting rhizobacteria HS11 and its application | |
| Wang et al. | Low-temperature straw biochar: Sustainable approach for sustaining higher survival of B. megaterium and managing phosphorus deficiency in the soil | |
| Feng et al. | Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L. | |
| Choi et al. | Effect of optical panel thickness for nutrient removal and cultivation of microalgae in the photobioreactor | |
| Zuo et al. | Microbial community structure analyses and cultivable denitrifier isolation of Myriophyllum aquaticum constructed wetland under low C/N ratio | |
| CN102776140A (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| CN205088059U (en) | Rural low contaminated water deep purification system | |
| CN108456108A (en) | The preparation method of micro- bio-feritlizer in a kind of supplement type | |
| CN105036482A (en) | Countryside low-pollution water deep purification system | |
| CN105132332B (en) | One strain of gluconacetobacter and its application as plant growth-promoting bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |