CN114105701A - Nutrient bacteriostatic slow-release ball for algae cultivation - Google Patents
Nutrient bacteriostatic slow-release ball for algae cultivation Download PDFInfo
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- CN114105701A CN114105701A CN202111363958.3A CN202111363958A CN114105701A CN 114105701 A CN114105701 A CN 114105701A CN 202111363958 A CN202111363958 A CN 202111363958A CN 114105701 A CN114105701 A CN 114105701A
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- Prior art keywords
- slow
- bacteriostatic
- ball
- nutrient
- release ball
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 87
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 48
- 230000003385 bacteriostatic effect Effects 0.000 title claims abstract description 34
- 239000000463 material Substances 0.000 claims abstract description 63
- 239000000843 powder Substances 0.000 claims abstract description 29
- 230000000813 microbial effect Effects 0.000 claims abstract description 21
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 14
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011230 binding agent Substances 0.000 claims abstract description 11
- 239000011573 trace mineral Substances 0.000 claims abstract description 10
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 10
- 239000011782 vitamin Substances 0.000 claims abstract description 10
- 235000013343 vitamin Nutrition 0.000 claims abstract description 10
- 229930003231 vitamin Natural products 0.000 claims abstract description 10
- 229940088594 vitamin Drugs 0.000 claims abstract description 10
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 9
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims abstract description 9
- 239000001110 calcium chloride Substances 0.000 claims abstract description 9
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 9
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims abstract description 9
- 235000011148 calcium chloride Nutrition 0.000 claims abstract description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 7
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims abstract description 7
- 235000019799 monosodium phosphate Nutrition 0.000 claims abstract description 7
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 7
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 7
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims abstract description 7
- 235000010344 sodium nitrate Nutrition 0.000 claims abstract description 7
- 239000004317 sodium nitrate Substances 0.000 claims abstract description 7
- 239000012798 spherical particle Substances 0.000 claims abstract description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 238000002156 mixing Methods 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 9
- 235000016709 nutrition Nutrition 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 8
- 230000035764 nutrition Effects 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 230000001112 coagulating effect Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 230000003311 flocculating effect Effects 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 239000004115 Sodium Silicate Substances 0.000 claims description 3
- 239000013505 freshwater Substances 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 claims description 2
- 239000001254 oxidized starch Substances 0.000 claims description 2
- 235000013808 oxidized starch Nutrition 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 238000004062 sedimentation Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 description 8
- 239000000203 mixture Substances 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 3
- 241000209094 Oryza Species 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241000206761 Bacillariophyta Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- GFHNAMRJFCEERV-UHFFFAOYSA-L cobalt chloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].[Cl-].[Co+2] GFHNAMRJFCEERV-UHFFFAOYSA-L 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 229960002413 ferric citrate Drugs 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- RWVGQQGBQSJDQV-UHFFFAOYSA-M sodium;3-[[4-[(e)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-n-ethyl-3-methylanilino]methyl]benzenesulfonate Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=C1 RWVGQQGBQSJDQV-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- FEJQDYXPAQVBCA-UHFFFAOYSA-J tetrasodium;ethane-1,2-diamine;tetraacetate Chemical compound [Na+].[Na+].[Na+].[Na+].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.NCCN FEJQDYXPAQVBCA-UHFFFAOYSA-J 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- KSLUMEQTEAUMJZ-UHFFFAOYSA-L zinc;sulfate;tetrahydrate Chemical compound O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O KSLUMEQTEAUMJZ-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/14—Tablets, spikes, rods, blocks or balls
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
Abstract
The invention provides a nutrient bacteriostatic slow-release ball for algae cultivation, which comprises a wrapping material, microbial powder and a nutrient medium, wherein the microbial powder and the nutrient medium are mixed and prepared into spherical particles of 0.5-0.8cm, the spherical particles are fermented for 5-9 days at 37 ℃ to obtain a mixed ball, a binder is sprayed on the surface of the mixed ball, and then the wrapping material and the mixed ball are mixed to obtain the slow-release ball; the nutrient medium comprises a base material and nutrients; the base material comprises brown sugar, framework material, yellow mud or sea mud; the nutrients comprise sodium nitrate or potassium nitrate, potassium dihydrogen phosphate or sodium dihydrogen phosphate, magnesium sulfate heptahydrate, calcium chloride, sodium bicarbonate, trace element solution, and vitamin solution. The bacteriostatic slow-release ball has the advantages of reasonable design, low cost, convenient use and good use effect.
Description
Technical Field
The invention relates to the technical field of algae cultivation, in particular to a nutrient bacteriostatic slow-release ball for algae cultivation.
Background
Algae is a low-grade aquatic plant and has cultivation significance due to its edible and medicinal values. In the initial stage of algae cultivation, a one-time fertilization mode is usually adopted to apply fertilizers into a water body, so that the fertilization precision is poor, and excessive fertilizers influence the growth and the propagation of algae in the water body and influence the quality of the algae. With the increasing understanding of people on algae, the method for culturing algae is gradually changed, the existing culture mode mainly depends on experience culture, and culture personnel continuously supplement nutrition to the water body by observing the growth condition of algae. However, due to different experience levels of culturists, conditions of insufficient or untimely supplement of nutrient elements are easily generated, and the growth and quality of algae are still adversely affected.
During the growth process of the algae, the growth of microorganisms is inevitably accompanied, and the growth of the microorganisms and the algae generate nutrition competition, so that the growth and the propagation of the algae are influenced. Therefore, the breeding personnel usually add the disinfectant into the water body for sterilization, a balanced ecological system exists in the water body, and in the process of adding the disinfectant for sterilization, the disinfectant has strong broad spectrum, poor pertinence to germs, is easy to destroy ecological balance and is not beneficial to long-acting bacteriostasis. Therefore, the method for adding the fertilizer for the algae cultivation is convenient and scientific, the utilization rate of the fertilizer is improved, and the improvement of the quality and the yield of the algae has important significance for the algae cultivation.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a nutrient bacteriostatic slow-release ball for algae cultivation, and particularly relates to a bacteriostatic slow-release ball comprising a wrapping material, microbial powder and a nutrient medium. After the bacteriostatic slow-release ball is put into a water body, nutrient elements in a nutrient medium can be continuously released to provide nutrition for the growth of algae, meanwhile, the existence of microbial bacteria can improve the ecological environment in the water body, the growth and the reproduction of germs are inhibited through the biological competition of beneficial bacteria, the disorder of an ecological system is avoided, and the long-term bacteriostatic effect is achieved. The bacteriostatic slow-release ball has the advantages of reasonable design, low cost, convenient use and good use effect.
The technical scheme of the invention is as follows:
a nutrition antibacterial slow release ball for algae cultivation comprises a wrapping material, microbial bacteria powder and a nutrient medium, wherein the microbial bacteria powder and the nutrient medium are mixed and made into spherical particles of 0.5-0.8cm, the spherical particles are fermented for 5-9 days at 37 ℃ to obtain a mixed ball, a binding agent is sprayed on the surface of the mixed ball, and then the wrapping material and the mixed ball are mixed to obtain a slow release ball;
the nutrient medium comprises a base material and nutrients;
the base material comprises brown sugar, framework material, yellow mud or sea mud;
the nutrients comprise sodium nitrate or potassium nitrate, potassium dihydrogen phosphate or sodium dihydrogen phosphate, magnesium sulfate heptahydrate, calcium chloride, sodium bicarbonate, trace element solution, and vitamin solution.
Preferably, the base material comprises the following components in parts by weight:
8-12 parts of brown sugar, 40-60 parts of framework material and 35-45 parts of yellow mud or sea mud.
Preferably, the framework material is one or a combination of more of rice hulls, corncob powder and sawdust.
Preferably, when the nutrient medium is prepared, the addition amount of the nutrients in each 100g of base stock is as follows:
0.5-1.0g of sodium nitrate or potassium nitrate, 0.15-0.30g of monopotassium phosphate or sodium dihydrogen phosphate, 0.5-0.8g of magnesium sulfate heptahydrate, 0.15-0.25g of calcium chloride, 0.3-0.5g of sodium bicarbonate, 0-0.5g of sodium silicate, 1ml of trace element solution and 1ml of vitamin solution; and uniformly mixing the base material and the nutrients to obtain the nutrient medium.
Preferably, when used in freshwater algae farming, yellow mud is used in the base material; when used for marine algae cultivation, sea mud is used as the base material.
Preferably, the weight ratio of the microbial powder to the nutrient medium is 2-5: 100.
Preferably, the viable count of the microbial powder is 50-100 hundred million/g.
When in use, the nutrient bacteriostatic slow-release balls are put into the water body according to the input amount of 10L of algae culture water body per 100g of nutrient medium.
Preferably, the preparation process of the wrapping material is as follows: drying the microalgae raw material until the water content is 8-12%, then crushing, sieving with a 90-110 mesh sieve, and taking undersize products as the wrapping materials.
Preferably, the microalgae raw material is obtained by filtering, flocculating and coagulating sedimentation treatment of waste microalgae in the culture process or naturally-growing waste microalgae; or the microalgae raw material is residue obtained after extracting protein, polysaccharide and/or oil.
Preferably, the microbial powder is EM (effective microorganism) powder.
The use of the microbial powder can enable microorganisms to generate a strong competitive action in water, effectively inhibit the growth and the propagation of harmful microorganisms, further improve the water environment of algae, degrade harmful substances such as ammoniacal nitrogen, methane, nitrite, hydrogen sulfide and the like in the water, maintain the pH stability of the water and keep the ecological balance of the aquaculture water.
Preferably, the binder is polypropylene waterproof binder or oxidized starch.
According to the invention, the algae wrapping material and the framework material enable the nutrient medium to be slowly released into the water body, so that the nutrient medium continuously provides nutrition for the growth of algae, meanwhile, the existence of a large amount of microbial bacteria generates competitive inhibition on harmful bacteria in the water body, a beneficial water body environment is maintained, and continuous and stable microecology is provided for the growth of algae.
Compared with the prior art, the invention has the beneficial effects that:
1. the nutrient bacteriostatic slow-release ball provided by the invention can continuously release C, N, P, K, Mg, Fe and other nutrient elements and microorganisms to a water body, so as to provide nutrient elements for the growth of algae, so that the algae can continuously and effectively absorb the nutrients in the water body and promote the growth of the algae; in addition, the competitive mechanism among the microorganisms is reasonably utilized to generate the bacteriostatic action on the harmful bacteria in the water body, thereby avoiding the use of chemical bactericides and maintaining the ecological balance of the water body environment.
2. The nutrient bacteriostatic slow-release ball provided by the invention is simple in preparation method, low in cost and suitable for popularization and application.
3. The nutrient bacteriostatic slow-release ball provided by the invention can be slowly released in a water body, the application scientificity of the fertilizer is improved, the waste of nutrient elements is avoided, the utilization rate of algae on the elements is improved, and the quality is improved.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the present invention, the composition of the trace element solution is as follows: 23mg of zinc sulfate tetrahydrate, 10mg of copper sulfate pentahydrate, 178mg of manganese chloride tetrahydrate, 3.9g of ferric citrate pentahydrate, 7.3mg of sodium molybdate dihydrate, 4.35g of ethylenediamine sodium tetraacetate, 12mg of cobalt chloride hexahydrate and 1000ml of pure water.
The composition of the vitamin solution was as follows: vitamin B120.5mg, 0.5mg of vitamin H (biotin), vitamin B1100mg, 1000ml of pure water.
The number of live bacteria in the microbial powder is 50 hundred million/g; the adhesive is polypropylene waterproof adhesive.
Example 1 seawater microalgae cultivation nutrient bacteriostatic slow-release ball
(1) Preparing a wrapping material: mechanically salvaging waste microalgae in the culture process, filtering, flocculating, coagulating and precipitating to obtain microalgae raw materials, drying the microalgae raw materials until the water content is 10 +/-0.5%, then crushing, sieving with a 100-mesh sieve, and taking undersize products as wrapping materials;
(2) preparing a base material: the composition of 1000g base material is: 100g of brown sugar, 200g of rice hull, 300g of sawdust and 400g of sea mud, and mixing the components of the base material for later use;
(3) preparing a nutrient medium: adding nutrients into the base material in the step (2), wherein the adding amount of the nutrients is as follows: 7g of sodium nitrate, 2.3g of monopotassium phosphate, 7g of magnesium sulfate heptahydrate, 2g of calcium chloride, 4g of sodium bicarbonate, 10ml of trace element solution and 10ml of vitamin solution; mixing to obtain nutrient medium;
(4) preparing a mixed ball: adding EM (effective microorganisms) powder into a nutrient medium according to the weight ratio of microbial powder to the nutrient medium of 3.5:100, uniformly mixing, adding into a disc granulator, rotating a disc to form small balls with the diameter of 0.5-0.8cm, and fermenting at 37 ℃ for 7 days to obtain mixed balls;
(5) spraying a binder on the surface of the mixed ball, and then mixing the wrapping material with the mixed ball to obtain a slow-release ball; adding 100g of nutrient bacteriostatic slow-release balls into every 10L of aquaculture water.
Example 2 nutritional bacteriostatic slow-release ball for fresh water microalgae culture
(1) Preparing a wrapping material: drying solid by-product algae residue obtained after extracting protein and oil as microalgae raw material until the water content is 9 + -0.5%, pulverizing, sieving with 90 mesh sieve, and taking undersize product as wrapping material;
(2) preparing a base material: the composition of 1000g base material is: 80g of brown sugar, 350g of corncob powder, 220g of sawdust and 350g of yellow mud, and mixing the components of the base material for later use;
(3) preparing a nutrient medium: adding nutrients into the base material in the step (2), wherein the adding amount of the nutrients is as follows: 7g of potassium nitrate, 2.3g of sodium dihydrogen phosphate, 7g of magnesium sulfate heptahydrate, 2g of calcium chloride, 4g of sodium bicarbonate, 10ml of trace element solution and 10ml of vitamin solution; mixing to obtain nutrient medium;
(4) preparing a mixed ball: adding EM (effective microorganisms) powder into a nutrient medium according to the weight ratio of the microbial powder to the nutrient medium of 4:100, uniformly mixing, adding into a disc granulator, rotating the disc to form small balls with the diameter of 0.5-0.8cm, and fermenting at 37 ℃ for 8 days to obtain mixed balls;
(5) spraying a binder on the surface of the mixed ball, and then mixing the wrapping material with the mixed ball to obtain a slow-release ball; adding 100g of nutrient bacteriostatic slow-release balls into every 10L of aquaculture water.
Example 3 seawater Chlorella cultivation nutrition bacteriostatic slow-release ball
(1) Preparing a wrapping material: mechanically salvaging waste microalgae in the culture process, filtering, flocculating, coagulating and precipitating to obtain microalgae raw materials, drying the microalgae raw materials until the water content is 11 +/-0.5%, then crushing, sieving with a 110-mesh sieve, and taking undersize products as wrapping materials;
(2) preparing a base material: the composition of 1000g base material is: 120g of brown sugar, 300g of rice hull, 130g of sawdust and 450g of sea mud, and mixing the components of the base material for later use;
(3) preparing a nutrient medium: adding nutrients into the base material in the step (2), wherein the adding amount of the nutrients is as follows: 5g of sodium nitrate, 1.5g of monopotassium phosphate, 5g of magnesium sulfate heptahydrate, 1.5g of calcium chloride, 3g of sodium bicarbonate, 10ml of trace element solution and 10ml of vitamin solution; mixing to obtain nutrient medium;
(4) preparing a mixed ball: adding EM (effective microorganisms) powder into a nutrient medium according to the weight ratio of the microbial powder to the nutrient medium of 2:100, uniformly mixing, adding into a disc granulator, rotating the disc to form small balls with the diameter of 0.5-0.8cm, and fermenting at 37 ℃ for 5 days to obtain mixed balls;
(5) spraying a binder on the surface of the mixed ball, and then mixing the wrapping material with the mixed ball to obtain a slow-release ball; adding 100g of nutrient bacteriostatic slow-release balls into every 10L of aquaculture water.
Example 4 nutrient bacteriostatic slow-release balls for diatom algae
(1) Preparing a wrapping material: mechanically salvaging waste microalgae in the culture process, filtering, flocculating, coagulating and precipitating to obtain microalgae raw materials, drying the microalgae raw materials until the water content is 9 +/-0.5%, then crushing, sieving with a 90-mesh sieve, and taking undersize products as wrapping materials;
(2) preparing a base material: the composition of 1000g base material is: 80g of brown sugar, 210g of corncob powder, 310g of sawdust and 400g of sea mud, and mixing the components of the base material for later use;
(3) preparing a nutrient medium: adding nutrients into the base material in the step (2), wherein the adding amount of the nutrients is as follows: 10g of potassium nitrate, 3g of sodium dihydrogen phosphate, 8g of magnesium sulfate heptahydrate, 2.5g of calcium chloride, 5g of sodium silicate, 5g of sodium bicarbonate, 10ml of trace element solution and 10ml of vitamin solution; mixing to obtain nutrient medium;
(4) preparing a mixed ball: adding EM (effective microorganisms) powder into a nutrient medium according to the weight ratio of the microbial powder to the nutrient medium of 5:100, uniformly mixing, adding into a disc granulator, rotating the disc to form small balls with the diameter of 0.5-0.8cm, and fermenting at 37 ℃ for 9 days to obtain mixed balls;
(5) spraying a binder on the surface of the mixed ball, and then mixing the wrapping material with the mixed ball to obtain a slow-release ball; adding 100g of nutrient bacteriostatic slow-release balls into every 10L of aquaculture water.
After the cultivation water is put in, the main nutrient components contained in the corresponding cultivation water bodies of the embodiments 1 to 4 are detected, and the results show that: within 5 days after the feeding, the slow release ball releases 20 percent of nutrient components; within 5-10 days, the release ball releases 40% of the nutrient components; within 11-20 days, the release ball releases 60% of nutrient components; within 21-30 days, the release ball releases 80% of the nutrient components; and by 60 days after the administration, the nutrient components of the release ball are basically completely released.
Comparative example 1
The difference from example 1 is that the mixed balls were thrown into the water body in such a manner that 100g of the mixed balls were thrown into 10L of the water body.
The growth conditions of the algae of examples 1 to 4 and comparative example 1 were observed, and it can be seen that the algae of examples 1 to 4 had a significant increase in algal density after 5 days and a stable water quality within 5 days; comparing the amount of the alga in the example 1 with the amount of the alga in the comparative example 1, the weight of the alga is improved by 36.6% compared with the comparative example 1, after the culture is finished, the amount of the alga in the example 1 is improved by 53.2% compared with the comparative example 1, and therefore, the utilization rate of the alga to a nutrient medium is improved by using the nutrient and bacteriostatic slow-release balls, and the alga culture effect is improved.
Although the present invention has been described in detail by referring to the preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A nutrition bacteriostatic slow-release ball for algae cultivation is characterized by comprising a wrapping material, microbial powder and a nutrient medium, wherein the microbial powder and the nutrient medium are mixed and prepared into spherical particles of 0.5-0.8cm, the spherical particles are fermented for 5-9 days at 37 ℃ to obtain a mixed ball, a binder is sprayed on the surface of the mixed ball, and then the wrapping material and the mixed ball are mixed to obtain a slow-release ball;
the nutrient medium comprises a base material and nutrients;
the base material comprises brown sugar, framework material, yellow mud or sea mud;
the nutrients comprise sodium nitrate or potassium nitrate, potassium dihydrogen phosphate or sodium dihydrogen phosphate, magnesium sulfate heptahydrate, calcium chloride, sodium bicarbonate, trace element solution, and vitamin solution.
2. The nutrient bacteriostatic slow-release ball for algae cultivation as claimed in claim 1, wherein the base material comprises the following components in parts by weight:
8-12 parts of brown sugar, 40-60 parts of framework material and 35-45 parts of yellow mud or sea mud.
3. The nutrient bacteriostatic slow-release ball for algae cultivation as claimed in claim 1, wherein the skeleton material is one or more of rice hull, corncob powder or sawdust.
4. The nutrient bacteriostatic slow-release ball for algae cultivation as claimed in claim 1, wherein when the nutrient medium is prepared, the addition amount of the nutrients in each 100g of base material is as follows:
0.5-1.0g of sodium nitrate or potassium nitrate, 0.15-0.30g of monopotassium phosphate or sodium dihydrogen phosphate, 0.5-0.8g of magnesium sulfate heptahydrate, 0.15-0.25g of calcium chloride, 0.3-0.5g of sodium bicarbonate, 0-0.5g of sodium silicate, 1ml of trace element solution and 1ml of vitamin solution; and uniformly mixing the base material and the nutrients to obtain the nutrient medium.
5. The vegetative bacteriostatic slow-release ball for algae cultivation according to claim 1, wherein yellow mud is used in the base material when used for freshwater algae cultivation; when used for marine algae cultivation, sea mud is used as the base material.
6. The vegetative bacteriostatic slow-release ball for algae cultivation according to claim 1, wherein the weight ratio of the microbial powder to the nutrient medium is 2-5: 100.
7. The nutrient bacteriostatic slow-release ball for algae cultivation as claimed in claim 1, wherein the viable count in the microbial powder is 50-100 hundred million/g.
8. The vegetative bacteriostatic slow-release ball for algae cultivation according to claim 1, wherein the preparation process of the wrapping material is as follows: drying the microalgae raw material until the water content is 8-12%, then crushing, sieving with a 90-110 mesh sieve, and taking undersize products as the wrapping materials.
9. The vegetative bacteriostatic slow-release ball for algae cultivation according to claim 1, wherein the microalgae raw material is obtained by filtering, flocculating and coagulating sedimentation treatment of waste microalgae in the cultivation process or naturally-growing waste microalgae; or the microalgae raw material is residue obtained after extracting protein, polysaccharide and/or oil.
10. The vegetative bacteriostatic slow-release ball for algae cultivation according to claim 1, wherein the microbial powder is EM (effective microorganism) powder; the binder is polypropylene waterproof binder or oxidized starch.
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