CN111592400A - Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof - Google Patents

Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof Download PDF

Info

Publication number
CN111592400A
CN111592400A CN202010380766.2A CN202010380766A CN111592400A CN 111592400 A CN111592400 A CN 111592400A CN 202010380766 A CN202010380766 A CN 202010380766A CN 111592400 A CN111592400 A CN 111592400A
Authority
CN
China
Prior art keywords
waterweed
powder
parts
fertilizer
bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202010380766.2A
Other languages
Chinese (zh)
Inventor
徐文杰
徐小马
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ma'anshan City Huinong Agricultural Science And Technology Development Co ltd
Original Assignee
Ma'anshan City Huinong Agricultural Science And Technology Development Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ma'anshan City Huinong Agricultural Science And Technology Development Co ltd filed Critical Ma'anshan City Huinong Agricultural Science And Technology Development Co ltd
Priority to CN202010380766.2A priority Critical patent/CN111592400A/en
Publication of CN111592400A publication Critical patent/CN111592400A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B13/00—Fertilisers produced by pyrogenic processes from phosphatic materials
    • C05B13/02—Fertilisers produced by pyrogenic processes from phosphatic materials from rock phosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/10—Solid or semi-solid fertilisers, e.g. powders
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/10—Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12—Granules or flakes

Abstract

The invention discloses a composite biological bacterial fertilizer for waterweed in a crab pool, which comprises base fertilizer particles A applied before waterweed cultivation and powder B applied in waterweed cultivation period. The invention also discloses a use method of the composite biological bacterial fertilizer for the waterweed in the crab pool, which comprises the steps of broadcasting the base fertilizer particles A in the whole pool before the waterweed is cultivated, and broadcasting the powder B in the whole pool after the waterweed is cultivated. According to the invention, the composite biological bacterial fertilizers with different formulations and different formulas are combined with different fertilizing modes, so that the nutrient required by the growth of the elodea nuttallii is satisfied, the environmental adaptability of the elodea nuttallii is improved, the growth state of the elodea nuttallii in the whole cultivation period is improved, and the yield of river crabs is effectively increased.

Description

Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof
Technical Field
The invention relates to the technical field of river crab culture, in particular to a composite biological bacterial fertilizer for waterweed in a crab pool and a using method thereof.
Background
The waterweed is commonly called as inedible and evergreen grass, has strong adaptability, is fresh, tender and crisp, is rich in nutritional ingredients such as protein, crude fiber, vitamin C, vitamin E, vitamin B12 and the like, calcium, phosphorus and various trace elements, is rich in nutrition, and is an excellent natural bait for river crabs. In addition, the waterweed can also be used as a place where river crabs inhabit and avoid molting, and has the effects of purifying water quality and increasing dissolved oxygen. Therefore, the growth state of the waterweed is extremely important for river crab culture, and the fertilization management is a key link influencing the growth of the waterweed. The fertilizer used by the waterweed is mostly chemical fertilizer and organic fertilizer, which easily causes water pollution and causes the waterweed to float and die. The biological bacterial fertilizer is a novel fertilizer, can provide fertility, can play a certain role in regulating water quality, and becomes a current research hotspot. However, the current biological bacterial manure cannot fully meet the required nutrition aiming at the characteristics of the waterweeds, and the effect of singly applying the biological bacterial manure is not ideal.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a composite biological bacterial fertilizer for waterweed in a crab pool and a using method thereof.
The invention provides a composite biological bacterial fertilizer for waterweed in a crab pool, which comprises base fertilizer particles A applied before waterweed cultivation and topdressing powder B applied in waterweed cultivation period;
the particle A comprises the following raw materials, by weight, 20-30 parts of mussel processing leftovers, 40-60 parts of bagasse, 5-10 parts of shell powder, 10-15 parts of calcium bentonite, 2-4 parts of sodium alginate, 0.4-0.8 part of compound vitamin, 0.3-0.6 part of trace element and a microbial agent A1-2 part, wherein the microbial agent A consists of azotobacter, phosphate solubilizing bacteria, lactobacillus and bacillus licheniformis according to the ratio of bacteria (1-2) to bacteria (1-3) to bacteria (4-10) and the total effective viable bacteria number is 0.5-1.5 × 109cfu/g；
The powder B comprises the following raw materials in parts by weight: 45-65 parts of tea leaf residues, 15-25 parts of seaweed powder, 10-15 parts of zeolite powder and humic acid3-5 parts of sodium, 5-10 parts of calcium magnesium phosphate fertilizer, 1-2 parts of potassium sulfate, 1-2 parts of urea, 1-2 parts of montmorillonite, 0.05-0.15 part of vitamin complex, 0.05-0.15 part of trace elements and a microbial agent B1-2, wherein the microbial agent B consists of photosynthetic bacteria, denitrifying bacteria and bacillus subtilis according to the ratio of bacteria (3-5) to bacteria (1-2), and the total effective viable count is 1 × 108-1×109cfu/g；
Preferably, the particle size of the particles A is 2-5 mm.
Preferably, the particle size of the powder B is 100-300 meshes.
Preferably, the preparation method of the particle A comprises the following steps:
(1) mixing mussel processing leftovers and bagasse, performing enzymolysis treatment by using a complex enzyme, and then inactivating enzyme at high temperature;
(2) adding shell powder, calcium bentonite, compound vitamins and trace elements into the material obtained in the step (1), uniformly mixing, then adding 1-2% sodium alginate aqueous solution, stirring for 1-3h at normal temperature, drying, crushing, extruding and granulating;
(3) and (3) adding the microbial agent A into a proper amount of water to prepare a bacterial suspension, uniformly spraying the bacterial suspension on the material obtained in the step (2), and carrying out ventilation drying to obtain the microbial agent A.
Preferably, in the step (1), the complex enzyme is prepared from cellulase, hemicellulase and neutral protease in a weight ratio of (1-3): (1-2): (1-2), the amount of the complex enzyme is 0.5-2% of the total weight of the mussel processing leftovers and the bagasse.
Preferably, in the step (1), the conditions of the enzymolysis treatment are as follows: the pH is 6-6.5, the temperature is 40-50 deg.C, and the enzymolysis time is 3-5 h.
Preferably, the preparation method of the powder B comprises the following steps: weighing the raw materials according to the weight, uniformly mixing the tea leaves, the seaweed powder, the zeolite powder and the microbial agent B, adjusting the water content to 40-50%, fermenting at 30-35 ℃ for 7-10 days, ventilating and drying, adding sodium humate, calcium magnesium phosphate fertilizer, potassium sulfate, urea, montmorillonite, compound vitamin and trace elements, uniformly mixing, crushing and sieving to obtain the compound fertilizer.
Preferably, the azotobacter is azotobacter chroococcum, the phosphate solubilizing bacteria are bacillus megaterium, the photosynthetic bacteria are rhodopseudomonas palustris, and the denitrifying bacteria are pseudomonas stutzeri.
The application method of the composite biological bacterial fertilizer for the waterweed in the crab pool comprises the following steps:
s1, broadcasting the base fertilizer particles A in the whole pool at 2-3 months, wherein the time for applying the particles A is 7-10 days before the beginning of the cultivation of the waterweed, and the application amount of the particles A is 100-150 kg/mu;
s2, spreading the powder B in the whole pond 3-7 days after the elodea nuttallii starts to be cultivated, then spreading the powder B in the whole pond every 30 days, wherein the application amount of the powder B is 10-20 kg/mu each time, and the application is stopped after 6 months.
The invention has the following beneficial effects:
the composite biological bacterial fertilizer for the waterweed in the crab pool comprises a base fertilizer particle A applied before waterweed cultivation and an additional fertilizer powder B applied in waterweed cultivation period, wherein the particle A is applied as a base fertilizer, and raw materials of the particle A comprise mussel processing leftovers, bagasse, shell powder, calcium bentonite, sodium alginate, composite vitamins, trace elements and a microbial agent A in proper proportion, wherein the shell powder and the calcium bentonite contain calcium ions and are slightly crosslinked with the sodium alginate, so that the composite biological bacterial fertilizer can play a certain nutrition slow-release role and is beneficial to constructing a granular microenvironment of bottom mud, the growth of beneficial microorganisms and waterweed root systems can be promoted, the release of toxins in the bottom mud can be inhibited, and the water quality can be improved; the mussel processing leftovers and the enzymolysis products of bagasse contain nutrient components such as a carbon source and a nitrogen source required by the growth of the elodea nutans, and also contain a large amount of growth promoting active substances such as polypeptides and polysaccharides, so that the growth of the elodea nutans is facilitated, and meanwhile, because the mussel processing leftovers and the enzymolysis products of the bagasse do not contain components such as chemical fertilizers and decomposed fertilizers, the eutrophication pollution of water bodies can be avoided, the growth environment of the elodea nutans is improved, and the rotting and death of the elo; the microbial agent A contains azotobacter, phosphate solubilizing bacteria, lactobacillus and bacillus licheniformis in proper proportion, can provide nutritional factors such as organic acid for promoting root growth and the like while improving the quality of bottom mud, and promotes the metabolism of root systems, so that the root systems of the waterweed are developed, the environmental adaptability of the waterweed is improved, and the death of floating roots of the waterweed is reduced; the powder B is used as a top dressing and comprises tea leaves, seaweed powder, zeolite powder, sodium humate, a calcium magnesium phosphate fertilizer, potassium sulfate, urea, montmorillonite, compound vitamins, trace elements and a microbial agent B in a proper proportion, wherein the calcium magnesium phosphate fertilizer, the potassium sulfate and the urea can play a role of quick-acting top dressing and quickly meet the growth requirement of the waterweed; after the tea leaves and the seaweed powder are fermented and decomposed, the basic carbon source and nitrogen source nutrition required by the growth of the elodea nuttallii can be quickly released, and a plurality of active factors such as tea polyphenol, tea polysaccharide, seaweed polysaccharide and the like for promoting the growth of the elodea nuttallii and improving the water environment can be provided; the microbial agent B contains photosynthetic bacteria, denitrifying bacteria and bacillus subtilis in proper proportion, can be quickly released into a water body, and plays a role in improving water quality. The granules mainly realize slow release of fertilizer efficiency, improve the quality of bottom mud and strengthen the roots of the elodea, the powder mainly realizes quick-acting fertilizer supply, improves the water environment and promotes the growth of the elodea, can avoid the overgrowth of the elodea due to overnutrition, can stably provide nutrient components necessary for the growth of the elodea for a long time, can improve the quality of the bottom quality and the water quality, strengthen the roots of the elodea, improve the environmental adaptability of the elodea, and improve the growth state of the elodea in the whole cultivation period, thereby effectively improving the yield of river crabs.
Detailed Description
The technical solution of the present invention will be described in detail below with reference to specific examples.
Example 1
The method for fertilizing the waterweeds comprises the following steps:
s1, broadcasting the base fertilizer particles A in the whole pond in the last ten days of 2 months, wherein the time for applying the particles A is 7 days before the Haylaria sp starts to be cultivated, and the application amount of the particles A is 100 kg/mu;
s2, spreading the powder B in the whole pond 3 days after the elodea nuttallii starts to be cultivated, then spreading the powder B in the whole pond every 30 days, wherein the application amount of the powder B is 10 kg/mu every time, and stopping applying after 6 months
The particle A comprises the following raw materials in parts by weight: mussel processing leftovers 20, bagasse 40, shell powder 5, calcium bentonite 10, sodium alginate 2, vitamin complex 0.4, trace elements 0.3 and microbial agent A1, wherein the microbial agent A isConsists of azotobacter, phosphate solubilizing bacteria, lactobacillus and bacillus licheniformis according to the bacteria number ratio of 1: 1: 4: 4, and the total effective viable bacteria number is 0.5 × 109cfu/g；
The preparation method of the particle A comprises the following steps:
(1) mixing mussel processing leftovers and bagasse, and performing enzymolysis treatment by using a complex enzyme, wherein the complex enzyme is prepared from cellulase, hemicellulase and neutral protease in a weight ratio of 1: 1: 1, the dosage of the complex enzyme is 0.5 percent of the total weight of the mussel processing leftovers and the bagasse, and the conditions of the enzymolysis treatment are as follows: performing enzymolysis at 40 deg.C and pH 6 for 3 hr, and inactivating enzyme at high temperature;
(2) adding shell powder, calcium bentonite, compound vitamins and trace elements into the material obtained in the step (1), uniformly mixing, then adding 1% sodium alginate aqueous solution, stirring for 1h at normal temperature, drying, crushing, extruding and granulating to obtain particles with the particle size of 2-5 mm;
(3) and (3) adding the microbial agent A into a proper amount of water to prepare a bacterial suspension, uniformly spraying the bacterial suspension on the particles obtained in the step (2), and carrying out ventilation drying to obtain the microbial agent A.
The powder B comprises the following raw materials, by weight, 45 parts of tea leaf residues, 15 parts of seaweed powder, 10 parts of zeolite powder, 3 parts of sodium humate, 5 parts of calcium magnesium phosphate fertilizer, 1 part of potassium sulfate, 1 part of urea, 1 part of montmorillonite, 0.05 part of compound vitamin, 0.05 part of trace element and 1 parts of microbial agent B, wherein the microbial agent B consists of photosynthetic bacteria, denitrifying bacteria and bacillus subtilis according to the bacteria number ratio of 3: 1: 1, and the total effective viable bacteria number is 1 × 108cfu/g；
The preparation method of the powder B comprises the following steps: weighing the raw materials according to the weight, uniformly mixing the tea leaves, the seaweed powder, the zeolite powder and the microbial agent B, adjusting the water content to 40%, fermenting for 7 days at 30 ℃, ventilating and drying, adding sodium humate, calcium magnesium phosphate fertilizer, potassium sulfate, urea, montmorillonite, compound vitamin and trace elements, uniformly mixing, crushing and sieving until the particle size is 100 meshes and 300 meshes.
Example 2
The method for fertilizing the waterweeds comprises the following steps:
s1, broadcasting the base fertilizer particles A in the whole pond in the last ten days of 3 months, wherein the time for applying the particles A is 10 days before the Haylaria sp starts to be cultivated, and the application amount of the particles A is 150 kg/mu;
s2, spreading the powder B in the whole pond 7 days after the elodea nuttallii starts to be cultivated, then spreading the powder B in the whole pond every 30 days, wherein the application amount of the powder B is 20 kg/mu every time, and stopping application after 6 months.
The particles A comprise the following raw materials, by weight, 30 parts of mussel processing leftovers, 60 parts of bagasse, 10 parts of shell powder, 15 parts of calcium-based bentonite, 4 parts of sodium alginate, 0.8 part of vitamin complex, 0.6 part of trace elements and 2 parts of microbial agent A, wherein the microbial agent A comprises azotobacter, phosphate solubilizing bacteria, lactobacillus and bacillus licheniformis according to the bacteria number ratio of 2: 3: 10: 10, and the total effective viable bacteria number is 1.5 × 109cfu/g；
The preparation method of the particle A comprises the following steps:
(1) mixing mussel processing leftovers and bagasse, and performing enzymolysis treatment by using a complex enzyme, wherein the complex enzyme is prepared from cellulase, hemicellulase and neutral protease in a weight ratio of 3: 2: 2, the dosage of the complex enzyme is 2 percent of the total weight of the mussel processing leftovers and the bagasse, and the conditions of the enzymolysis treatment are as follows: the pH value is 6.5, the temperature is 50 ℃, the enzymolysis time is 5h, and then the enzyme is deactivated at high temperature;
(2) adding shell powder, calcium bentonite, compound vitamins and trace elements into the material obtained in the step (1), uniformly mixing, then adding 2% sodium alginate aqueous solution, stirring for 3 hours at normal temperature, drying, crushing, extruding and granulating to obtain particles with the particle size of 2-5 mm;
(3) and (3) adding the microbial agent A into a proper amount of water to prepare a bacterial suspension, uniformly spraying the bacterial suspension on the particles obtained in the step (2), and carrying out ventilation drying to obtain the microbial agent A.
The powder B comprises the following raw materials, by weight, 65 parts of tea leaf residues, 25 parts of seaweed powder, 15 parts of zeolite powder, 5 parts of sodium humate, 10 parts of calcium magnesium phosphate fertilizer, 2 parts of potassium sulfate, 2 parts of urea, 2 parts of montmorillonite, 0.15 part of compound vitamin, 0.15 part of trace element and 2 parts of microbial agent B, wherein the microbial agent B consists of photosynthetic bacteria, denitrifying bacteria and bacillus subtilis according to the bacteria number ratio of 5: 2: 2, and the total effective viable bacteria number is 1 × 109cfu/g；
The preparation method of the powder B comprises the following steps: weighing the raw materials according to the weight, uniformly mixing the tea leaves, the seaweed powder, the zeolite powder and the microbial agent B, adjusting the water content to 50%, then fermenting for 10 days at 35 ℃, ventilating and drying, adding sodium humate, calcium magnesium phosphate fertilizer, potassium sulfate, urea, montmorillonite, compound vitamin and trace elements, uniformly mixing, crushing and sieving until the particle size is 300 meshes, thus obtaining the compound vitamin A fertilizer.
Example 3
The method for fertilizing the waterweeds comprises the following steps:
s1, broadcasting the base fertilizer particles A in the whole pond in the last ten days of 2 months, wherein the time for applying the particles A is 8 days before the Haylaria sp starts to be cultivated, and the application amount of the particles A is 120 kg/mu;
s2, spreading the powder B in the whole pond 5 days after the elodea nuttallii starts to be cultivated, then spreading the powder B in the whole pond every 30 days, wherein the application amount of the powder B is 15 kg/mu every time, and stopping application after 6 months.
The particles A comprise the following raw materials, by weight, 25 parts of mussel processing leftovers, 50 parts of bagasse, 8 parts of shell powder, 12 parts of calcium-based bentonite, 2.5 parts of sodium alginate, 0.5 part of vitamin complex, 0.5 part of trace elements and 1.5 parts of microbial agent A, wherein the microbial agent A comprises azotobacter, phosphate solubilizing bacteria, lactobacillus and bacillus licheniformis according to the bacteria number ratio of 1.5: 1.5: 7.5: 7.5, and the total effective viable bacteria number is 1 × 109cfu/g；
The preparation method of the particle A comprises the following steps:
(1) mixing mussel processing leftovers and bagasse, and performing enzymolysis treatment by using a complex enzyme, wherein the complex enzyme is prepared from cellulase, hemicellulase and neutral protease in a weight ratio of 2: 1.5: 1.5, the dosage of the complex enzyme is 1 percent of the total weight of the mussel processing leftovers and the bagasse, and the conditions of the enzymolysis treatment are as follows: the pH value is 6.4, the temperature is 45 ℃, the enzymolysis time is 4h, and then the enzyme is deactivated at high temperature;
(2) adding shell powder, calcium bentonite, compound vitamins and trace elements into the material obtained in the step (1), uniformly mixing, then adding 1.5% sodium alginate aqueous solution, stirring for 2 hours at normal temperature, drying, crushing, extruding and granulating to prepare particles with the particle size of 2-5 mm;
(3) and (3) adding the microbial agent A into a proper amount of water to prepare a bacterial suspension, uniformly spraying the bacterial suspension on the particles obtained in the step (2), and carrying out ventilation drying to obtain the microbial agent A.
The powder B comprises the following raw materials, by weight, 50 parts of tea leaf residues, 20 parts of seaweed powder, 12 parts of zeolite powder, 4 parts of sodium humate, 7.5 parts of calcium magnesium phosphate fertilizer, 1.5 parts of potassium sulfate, 1.5 parts of urea, 1.8 parts of montmorillonite, 0.1 part of compound vitamin, 0.1 part of trace elements and 1.5 parts of microbial agent B, wherein the microbial agent B consists of photosynthetic bacteria, denitrifying bacteria and bacillus subtilis according to the bacteria number ratio of 4: 1.5: 1.5, and the total effective viable bacteria number is 5 × 108cfu/g；
The preparation method of the powder B comprises the following steps: weighing the raw materials according to the weight, uniformly mixing the tea leaves, the seaweed powder, the zeolite powder and the microbial agent B, adjusting the water content to 45%, fermenting for 8 days at 32 ℃, ventilating and drying, adding sodium humate, calcium magnesium phosphate fertilizer, potassium sulfate, urea, montmorillonite, compound vitamin and trace elements, uniformly mixing, crushing and sieving until the particle size is 100 meshes and 300 meshes.
Comparative example 1
The method for fertilizing the waterweeds comprises the following steps:
s1, starting to broadcast the base fertilizer in the whole pond in the last ten days of 2 months, wherein the time for applying the base fertilizer is 8 days before the Haylaria pallida starts to be cultivated, and the application amount of the base fertilizer is 120 kg/mu;
s2, applying top dressing in the whole pond 5 days after the cultivation of the waterweeds is started, then applying top dressing in the whole pond every 30 days, wherein the application amount of the top dressing is 15 kg/mu each time, and stopping applying after 6 months.
Wherein, the base fertilizer and the additional fertilizer both adopt the particle A, and the formula and the preparation method of the particle A are the same as those of the embodiment 3.
Comparative example 2
The method for fertilizing the waterweeds comprises the following steps:
s1, starting to broadcast the base fertilizer in the whole pond in the last ten days of 2 months, wherein the time for applying the base fertilizer is 8 days before the Haylaria pallida starts to be cultivated, and the application amount of the base fertilizer is 120 kg/mu;
s2, applying top dressing in the whole pond 5 days after the cultivation of the waterweeds is started, then applying top dressing in the whole pond every 30 days, wherein the application amount of the top dressing is 15 kg/mu each time, and stopping applying after 6 months.
Wherein, the base fertilizer and the additional fertilizer both adopt powder B, and the formula and the preparation method of the powder B are the same as those of the embodiment 3.
Comparative example 3
The conventional method for fertilizing the waterweeds comprises the following specific steps:
s1, spreading base fertilizer in the whole pond in late 2 months for 8 days before the Haylaria sp begins to be cultivated, wherein the base fertilizer comprises organic fertilizer 150 kg/mu and effective viable count not less than 5 × 1082 kg/mu of cfu/g of biological bacterial manure;
s2, applying additional fertilizer to the whole pond 5 days after the Haylaria pallida starts to be cultivated, and then applying additional fertilizer to the whole pond every 30 days, wherein the additional fertilizer comprises 30 kg/mu of organic fertilizer, and the number of commercially available effective viable bacteria is more than or equal to 5 × 108The application of cfu/g of biological bacterial manure is 0.5 kg/mu, and the application is stopped after 6 months.
The cultivation of the elodea and the culture test of the river crab:
the method for fertilizing the waterweeds in the embodiment 3 and the comparative examples 1-3 of the invention are respectively adopted to carry out waterweeds cultivation and river crab culture tests, and the test pond conditions are as follows: the area of the pond is 18 mu, escape-proof facilities with the height of 50cm are arranged around the pond, and a water inlet is filtered by a nylon mesh bag. The method for cultivating the waterweeds comprises the following steps: cutting and planting the waterweeds into sections, and planting 20kg of waterweeds in each mu of area; the crab spawn feeding method comprises the following steps: putting Chinese mitten crab seeds in the last ten days of 4 months, wherein the putting amount is 600 crab seeds/mu; the feed feeding method comprises the following steps: 450 kg/mu of river snails are put in the feed, and the mixed feed is fed every day, wherein the daily feeding amount is 3 percent of the crab weight. Fishing and weighing in the last ten days of 11 months. The per mu yield of eriocheir sinensis is shown in table 1:
TABLE 1 Eriocheir sinensis yield per mu
Figure BDA0002481974520000091
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. The composite biological bacterial fertilizer for the waterweed in the crab pool is characterized by comprising a base fertilizer particle A applied before waterweed cultivation and a powder B applied in the waterweed cultivation period;
the particle A comprises the following raw materials, by weight, 20-30 parts of mussel processing leftovers, 40-60 parts of bagasse, 5-10 parts of shell powder, 10-15 parts of calcium bentonite, 2-4 parts of sodium alginate, 0.4-0.8 part of compound vitamin, 0.3-0.6 part of trace element and a microbial agent A1-2 part, wherein the microbial agent A consists of azotobacter, phosphate solubilizing bacteria, lactobacillus and bacillus licheniformis according to the ratio of bacteria (1-2) to bacteria (1-3) to bacteria (4-10) and the total effective viable bacteria number is 0.5-1.5 × 109cfu/g；
The powder B comprises the following raw materials, by weight, 45-65 parts of tea leaf residues, 15-25 parts of seaweed powder, 10-15 parts of zeolite powder, 3-5 parts of sodium humate, 5-10 parts of calcium magnesium phosphate fertilizer, 1-2 parts of potassium sulfate, 1-2 parts of urea, 1-2 parts of montmorillonite, 0.05-0.15 part of vitamin complex, 0.05-0.15 part of trace elements and a microbial agent B1-2 part, wherein the microbial agent B consists of photosynthetic bacteria, denitrifying bacteria and bacillus subtilis according to the ratio of bacteria (3-5) to bacteria (1-2), and the total effective viable count is 1 × 108-1×109cfu/g。
2. The composite biological bacterial fertilizer for waterweed in crab pool of claim 1, wherein the particle size of the particle A is 2-5 mm.
3. The composite biological bacterial fertilizer for waterweed in crab pool as claimed in claim 1 or 2, wherein the particle size of the powder B is 100-300 mesh.
4. The composite biological bacterial fertilizer for waterweed in crab pool as claimed in any one of claims 1-3, wherein the preparation method of the granule A comprises the following steps:
(1) mixing mussel processing leftovers and bagasse, performing enzymolysis treatment by using a complex enzyme, and then inactivating enzyme at high temperature;
(2) adding shell powder, calcium bentonite, compound vitamins and trace elements into the material obtained in the step (1), uniformly mixing, then adding 1-2% sodium alginate aqueous solution, stirring for 1-3h at normal temperature, drying, crushing, extruding and granulating;
(3) and (3) adding the microbial agent A into a proper amount of water to prepare a bacterial suspension, uniformly spraying the bacterial suspension on the material obtained in the step (2), and carrying out ventilation drying to obtain the microbial agent A.
5. The composite biological bacterial fertilizer for waterweed in crab pool according to claim 4, wherein in the step (1), the composite enzyme is prepared from cellulase, hemicellulase and neutral protease according to the weight ratio of (1-3): (1-2): (1-2), the amount of the complex enzyme is 0.5-2% of the total weight of the mussel processing leftovers and the bagasse.
6. The composite biological bacterial fertilizer for waterweed in crab pool as claimed in claim 4 or 5, wherein in the step (1), the condition of enzymolysis is as follows: the pH is 6-6.5, the temperature is 40-50 deg.C, and the enzymolysis time is 3-5 h.
7. The composite biological bacterial fertilizer for waterweed in crab pool according to any one of claims 1-6, wherein the preparation method of the powder B comprises the following steps: weighing the raw materials according to the weight, uniformly mixing the tea leaves, the seaweed powder, the zeolite powder and the microbial agent B, adjusting the water content to 40-50%, fermenting at 30-35 ℃ for 7-10 days, ventilating and drying, adding sodium humate, calcium magnesium phosphate fertilizer, potassium sulfate, urea, montmorillonite, compound vitamin and trace elements, uniformly mixing, crushing and sieving to obtain the compound fertilizer.
8. The use method of the composite biological bacterial fertilizer for the waterweed in the crab pool as claimed in any one of claims 1 to 7, which comprises the following steps:
s1, broadcasting the base fertilizer particles A in the whole pool at 2-3 months, wherein the time for applying the particles A is 7-10 days before the beginning of the cultivation of the waterweed, and the application amount of the particles A is 100-150 kg/mu;
s2, spreading the powder B in the whole pond 3-7 days after the elodea nuttallii starts to be cultivated, then spreading the powder B in the whole pond every 30 days, wherein the application amount of the powder B is 10-20 kg/mu each time, and the application is stopped after 6 months.
CN202010380766.2A 2020-05-08 2020-05-08 Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof Withdrawn CN111592400A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010380766.2A CN111592400A (en) 2020-05-08 2020-05-08 Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010380766.2A CN111592400A (en) 2020-05-08 2020-05-08 Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof

Publications (1)

Publication Number Publication Date
CN111592400A true CN111592400A (en) 2020-08-28

Family

ID=72186815

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010380766.2A Withdrawn CN111592400A (en) 2020-05-08 2020-05-08 Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof

Country Status (1)

Country Link
CN (1) CN111592400A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116855430A (en) * 2023-09-05 2023-10-10 中国农业科学院农业环境与可持续发展研究所 Composite microbial agent for promoting corrosion and increasing yield of rice field straw and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116855430A (en) * 2023-09-05 2023-10-10 中国农业科学院农业环境与可持续发展研究所 Composite microbial agent for promoting corrosion and increasing yield of rice field straw and application thereof

Similar Documents

Publication Publication Date Title
CN101602623B (en) Microbial organic fertilizer and compound fertilizer, and preparation methods thereof
CN103058771B (en) Controlled-release and slow-release compound fertilizer specialized for kiwi fruit
CN102875236B (en) Total nutrient organic-inorganic compound fertilizer
CN102633559B (en) Activated carbon slow-release zinc fertilizer and preparation method thereof
CN111302856B (en) Water-soluble organic biological bacterial fertilizer for drip irrigation crops and preparation method thereof
WO2019091141A1 (en) Dedicated controlled-release coated fertilizer for peanuts in saline-alkali soil, and preparation method therefor
CN106278515A (en) A kind of spacetabs type micro organism composite fertilizer and preparation method thereof
CN111960890A (en) Wormcast organic fertilizer and preparation method thereof
CN113214000A (en) Composite microbial fertilizer and preparation method thereof
CN111285742A (en) Special biofertilizer for moderate and severe saline-alkali soil and preparation method thereof
CN110437000A (en) A kind of microbial manure and its preparation method and application
CN112125745A (en) Biomass organic fertilizer for improving soil and preparation method thereof
CN112811952A (en) Fertilizer beneficial to growth of soil microorganisms and preparation method thereof
CN111592400A (en) Composite biological bacterial fertilizer for waterweed in crab pool and using method thereof
CN111848270A (en) Compound fertilizer containing biological enzyme and preparation method thereof
CN113896595A (en) Multi-element water-soluble compound microbial fertilizer and preparation method thereof
CN106986702A (en) Carbon energy bio-fertilizer and preparation method thereof
CN112028692A (en) Special biological organic fertilizer for saline-alkali soil and preparation method thereof
CN113292373A (en) Special fertilizer for rice and preparation method thereof
CN1260184C (en) Preparation method of high efficient biological organic fertilizer
CN113292386A (en) Complete-element biological organic fertilizer and preparation method thereof
CN111083979B (en) Method for producing selenium-rich potatoes by supplementing exogenous selenium through foliage spraying
CN114702352B (en) Composite microbial fertilizer and preparation method and application thereof
CN101456760A (en) Cenotype microbial manure prepared by cattle manure and using Chinese medicine to adjust cattle enteric microorganisms structure
CN106699465A (en) Method of preparing novel green high-efficiency organic and inorganic compound formula fertilizer

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20200828