CN114053383A - Fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea and preparation method thereof - Google Patents
Fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea and preparation method thereof Download PDFInfo
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Abstract
The invention provides a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, and relates to the field of fermented traditional Chinese medicine preparations. The traditional Chinese medicine composition comprises six traditional Chinese medicinal materials of astragalus, liquorice, madder, purslane, alpinia oxyphylla and poplar flower, and the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis. According to the invention, different microorganisms are reasonably proportioned, so that the number of beneficial microorganisms in intestinal tracts of piglets can be increased, the intestinal mucosa can be repaired conveniently, and meanwhile, the effective components and active substances of traditional Chinese medicines can be extracted and utilized to the greatest extent through the fermentation of the composite microorganisms, so that the absorption rate of the piglets is increased. The invention can effectively prevent and treat diarrhea of piglets, remarkably reduce the death rate, has positive effect on weight increment of piglets and promotes the growth and development of piglets.
Description
Technical Field
The invention relates to the field of fermented traditional Chinese medicine preparations, in particular to a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea and a preparation method thereof.
Background
In the traditional breeding industry, the genetic breeding improvement of the pigs focuses more on the improvement of growth and reproductive performance, but the disease resistance is not obviously improved. The diarrhea of the piglets is one of the most common diseases in the breeding industry, is one of the reasons influencing the development of the pig industry, can commonly occur the diseases, has wide prevalence, has slow growth speed after the disease occurrence, is easy to generate other secondary infectious diseases because the diarrhea causes the physique decline and the immunologic function decline, greatly weakens the resistance to the epidemic diseases, seriously causes the higher mortality of the piglets, and causes great loss to the pig industry.
Antibiotics or synthetic antibacterial drugs are mainly adopted in the aspect of piglet diarrhea drug treatment at the present stage, but drug-resistant bacteria can be caused by long-term use of the drugs, so that the piglet diarrhea treatment difficulty is increased. The biological feed as a live bacterial preparation plays an active role in improving the growth performance and the production performance of pigs and preventing and treating diseases, and can improve the digestibility of the feed while ensuring the balance of the flora in intestinal tracts of piglets and improving the immunity of the intestinal tracts. The Chinese herbal medicine contains natural active substances, has the advantages of no drug resistance, small toxic and side effects, no drug residue and the like compared with antibiotics, and can enhance the resistance of livestock and poultry and improve the immunity of the livestock and poultry.
The modern Chinese medicine fermentation technology is a novel Chinese medicine fermentation process formed by combining modern microecology, fermentation engineering technology, bioengineering and the like on the basis of the traditional Chinese medicine fermentation. At present, a large number of test results show that the modern traditional Chinese medicine fermentation technology can improve the curative effect to a greater extent, change the performance of the medicine and expand the indications of the medicine. Meanwhile, enzymes with various effects generated by microbial growth and metabolism may decompose and convert certain components of traditional Chinese medicines into new effective components or decompose toxic components to reduce toxic and side effects of the medicines. The fermented traditional Chinese medicine microecologics have a lot of research on the influence of the fermented traditional Chinese medicine microecologics on the production performance of live pigs, and a large number of literature reports show that the fermented traditional Chinese medicine microecologics can obviously improve the production performance of the live pigs, but the research on the influence of the fermented microecologics on the diarrhea of piglets is not many.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea and a preparation method thereof.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
on one hand, the invention provides a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, which comprises a traditional Chinese medicine composition and a compound microorganism; the traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus crude extract, 5-10 parts of licorice root crude extract, 5-15 parts of madder root crude extract, 10-15 parts of purslane crude extract, 10-20 parts of fructus alpiniae oxyphyllae crude extract and 5-10 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5-1: 1-2: 0.5-2: 1-2: 0.5 to 2.
Further, as an optimization of a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, the traditional Chinese medicine composition comprises the following components in parts by weight: 14-18 parts of astragalus membranaceus crude extract, 5-8 parts of licorice root crude extract, 8-14 parts of madder root crude extract, 12-15 parts of purslane crude extract, 12-16 parts of fructus alpiniae oxyphyllae crude extract and 5-8 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5-0.8: 1.2-1.8: 0.8-1.6: 1.2-1.6: 0.8 to 1.6.
Further, as an optimization of a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, the traditional Chinese medicine composition comprises the following components in parts by weight: 15 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 10 parts of madder crude extract, 15 parts of purslane crude extract, 14 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.6: 1.6: 1: 1.5: 1.2.
on the other hand, the invention also provides a preparation method of the fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, which comprises the following steps:
(1) selecting genuine medicinal materials such as astragalus, liquorice, madder, purslane, fructus alpiniae oxyphyllae and poplar flower, respectively carrying out immersion extraction for 2 times, then carrying out spray drying on extract liquor, and sieving with a 200-mesh sieve for later use after crushing;
(2) clostridium butyricum, Bacillus subtilis, Lactobacillus acidophilus,Respectively inoculating the bacterial liquid of bacillus geotrichum and enterococcus faecalis into respective liquid culture medium in an inoculation amount of 2%, fermenting at 30-37 deg.C for 36-72 hr to obtain each strain culture liquid, wherein the viable bacteria number of each strain culture is 0.5 × 1010CFU/ml～1.0×1012CFU/ml；
(3) Accurately weighing the traditional Chinese medicine extracts in the step (1) according to the following parts by weight: 10-20 parts of astragalus membranaceus crude extract, 5-10 parts of licorice root crude extract, 5-15 parts of madder root crude extract, 10-15 parts of purslane crude extract, 10-20 parts of fructus alpiniae oxyphyllae crude extract and 5-10 parts of poplar flower crude extract, and uniformly mixing to obtain a traditional Chinese medicine composition for later use;
(3) mixing the traditional Chinese medicine composition with each strain culture solution obtained in the step (2) according to the proportion of adding 30mL of strain culture solution into every 150g of the traditional Chinese medicine composition, and performing closed anaerobic fermentation at the fermentation temperature of 30-37 ℃ for 48-72 hours to obtain fermentation products of each strain for later use;
(4) and (3) fermenting products of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis obtained in the step (3) according to the viable bacteria number ratio of 0.5-1: 1-2: 0.5-2: 1-2: 0.5-2, uniformly stirring, and freeze-drying to finally obtain the fermented traditional Chinese medicine microecological preparation.
(III) advantageous effects
The microbial ecological agent for treating piglet diarrhea provided by the invention is prepared by fermenting a traditional Chinese medicine composition and a compound microorganism by using a modern fermentation technology. The invention utilizes the characteristic that traditional Chinese medicines are not easy to generate drug resistance and drug residue of animal products, selects six traditional Chinese medicinal materials of astragalus, liquorice, madder, purslane, alpinia oxyphylla and poplar flower, and is prepared by scientifically mixing various microorganisms such as clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis and fermenting the traditional Chinese medicines. According to the invention, different microorganisms are reasonably proportioned, so that the number of beneficial microorganisms in intestinal tracts of piglets can be increased, the intestinal mucosa can be repaired conveniently, and meanwhile, the effective components and active substances of traditional Chinese medicines can be extracted and utilized to the greatest extent through the fermentation of the composite microorganisms, so that the absorption rate of the piglets is increased. The invention can effectively prevent and treat diarrhea of piglets, remarkably reduce the death rate, has positive effect on weight increment of piglets and promotes the growth and development of piglets.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea is prepared according to the following steps:
(1) selecting genuine medicinal materials such as astragalus, liquorice, madder, purslane, fructus alpiniae oxyphyllae and poplar flower, respectively carrying out immersion extraction for 2 times, then carrying out spray drying on extract liquor, and sieving with a 200-mesh sieve for later use after crushing;
(2) respectively inoculating the bacterial liquid of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis into respective liquid culture medium by the inoculation amount of 2%, fermenting for 36-72 hours at 30-37 ℃ to obtain each strain culture liquid, wherein the viable bacteria number of each strain culture is 0.5 multiplied by 1010CFU/ml～1.0×1012CFU/ml；
(3) Accurately weighing the traditional Chinese medicine extracts in the step (1) according to the following parts by weight: 10 parts of astragalus membranaceus crude extract, 5 parts of licorice root crude extract, 5 parts of madder root crude extract, 10 parts of purslane crude extract, 10 parts of alpinia oxyphylla crude extract and 5 parts of poplar flower crude extract, and uniformly mixing to obtain a traditional Chinese medicine composition for later use;
(3) mixing the traditional Chinese medicine composition with each strain culture solution obtained in the step (2) according to the proportion of adding 30mL of strain culture solution into every 150g of the traditional Chinese medicine composition, and performing closed anaerobic fermentation at the fermentation temperature of 30-37 ℃ for 48-72 hours to obtain fermentation products of each strain for later use;
(4) and (3) fermenting products of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis obtained in the step (3) according to the viable bacteria quantity ratio of 0.5: 1: 0.5: 1: 0.5, uniformly stirring, and freeze-drying to finally obtain the fermented traditional Chinese medicine microecological preparation.
Example 2
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20 parts of astragalus membranaceus crude extract, 10 parts of liquorice crude extract, 15 parts of madder crude extract, 15 parts of purslane crude extract, 20 parts of fructus alpiniae oxyphyllae crude extract and 10 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 1: 2: 2: 2: 2.
example 3
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 14 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 8 parts of madder crude extract, 12 parts of purslane crude extract, 12 parts of fructus alpiniae oxyphyllae crude extract and 5 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5: 1.2: 0.8: 1.2: 0.8.
example 4
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 18 parts of radix astragali crude extract, 8 parts of licorice crude extract, 14 parts of madder crude extract, 15 parts of purslane crude extract, 16 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.8: 1.8: 1.6: 1.6: 1.6.
example 5
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 10 parts of madder crude extract, 15 parts of purslane crude extract, 14 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.6: 1.6: 1: 1.5: 1.2.
in order to further illustrate the treatment effect of the fermented traditional Chinese medicine microecological preparation on piglet diarrhea, an animal experiment is provided as an illustration below; the fermented traditional Chinese medicine microecologics mentioned below were prepared according to example 5.
1 materials and methods
1.1 test drugs, test animals
Test drugs: the fermented traditional Chinese medicine micro-ecological preparation prepared in example 5, florfenicol soluble powder.
Test animals: 60 weaned piglets suffering from diarrhea and about 20 days old are selected from the pig group.
1.2 test methods
Randomly dividing 60 weaned piglets suffering from diarrhea into 3 groups, wherein each group comprises 20 piglets, namely a blank group, a positive control group and a test group; healthy and disease-free 20 piglets are taken as a prevention group; the blank group is administrated with 2mL of physiological saline per kilogram of piglet weight; the positive control group is administrated with florfenicol soluble powder 15mg per kilogram of piglet weight; the experimental group is administrated with 15mg of the fermented traditional Chinese medicine microecological preparation per kilogram of piglet weight; during the test period, all the test piglets are raised under the same condition, are fed freely, drink freely and are fed with less feeding; the mental, fecal and appetite status and death status of the piglets are observed every day, the treatment process is carried out until the piglets are cured or die, and the test is also terminated if the piglets are not cured or die within one week.
1.3 therapeutic efficacy determination
The method has the following advantages: the shape of the excrement is improved, the diarrhea frequency is reduced, and the food intake is increased;
and (3) curing: the food intake is normal, the mental state is good, and the diarrhea symptom disappears;
and (4) invalidation: food intake and diarrhea symptoms did not improve, and diarrhea symptoms even worsened, or died.
2 results
Table 2 shows the treatment results of the piglets of each group during the trial. As can be seen from the results in Table 2, the mortality rate of piglets suffering from diarrhea is as high as 65% without drug treatment, which indicates that the diarrhea of piglets has serious harm to the growth of piglets; compared with the blank group, after the piglets are treated, the florfenicol soluble powder and the fermented traditional Chinese medicine microecological preparation have obvious treatment effect on the diarrhea of the piglets, and the effective rate can reach 85 percent; compared with a positive control group, the effective rate of the test group on the treatment of the piglet diarrhea can reach 100 percent, is obviously higher than that of the positive control group, the cure rate can reach 90 percent, is also obviously higher than that of the positive control group, and meanwhile, the test group has no dead piglet cases. The test results show that the treatment effect of the fermented traditional Chinese medicine microecological preparation for treating the diarrhea of the piglets is better than the effective rate and the cure rate of the florfenicol soluble powder for treating the diarrhea of the piglets, the death of the piglets caused by the diarrhea can be effectively avoided, and the fermented traditional Chinese medicine microecological preparation can replace antibiotics to treat the diarrhea of the piglets.
TABLE 2
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (4)
1. A fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea is characterized by comprising a traditional Chinese medicine composition and a compound microorganism; the traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus crude extract, 5-10 parts of licorice root crude extract, 5-15 parts of madder root crude extract, 10-15 parts of purslane crude extract, 10-20 parts of fructus alpiniae oxyphyllae crude extract and 5-10 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5-1: 1-2: 0.5-2: 1-2: 0.5 to 2.
2. The fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea according to claim 1, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 14-18 parts of astragalus membranaceus crude extract, 5-8 parts of licorice root crude extract, 8-14 parts of madder root crude extract, 12-15 parts of purslane crude extract, 12-16 parts of fructus alpiniae oxyphyllae crude extract and 5-8 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5-0.8: 1.2-1.8: 0.8-1.6: 1.2-1.6: 0.8 to 1.6.
3. The fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea according to claim 1, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 15 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 10 parts of madder crude extract, 15 parts of purslane crude extract, 14 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.6: 1.6: 1: 1.5: 1.2.
4. the preparation method of the fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea according to any one of claims 1 to 3, wherein the preparation method comprises the following steps:
(1) selecting genuine medicinal materials such as astragalus, liquorice, madder, purslane, fructus alpiniae oxyphyllae and poplar flower, respectively carrying out immersion extraction for 2 times, then carrying out spray drying on extract liquor, and sieving with a 200-mesh sieve for later use after crushing;
(2) respectively inoculating the bacterial liquid of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis into respective liquid culture medium by the inoculation amount of 2%, fermenting for 36-72 hours at 30-37 ℃ to obtain each strain culture liquid, wherein the viable bacteria number of each strain culture is 0.5 multiplied by 1010CFU/mL～1.0×1012CFU/mL；
(3) Accurately weighing the traditional Chinese medicine extracts in the step (1) according to the following parts by weight: 10-20 parts of astragalus membranaceus crude extract, 5-10 parts of licorice root crude extract, 5-15 parts of madder root crude extract, 10-15 parts of purslane crude extract, 10-20 parts of fructus alpiniae oxyphyllae crude extract and 5-10 parts of poplar flower crude extract, and uniformly mixing to obtain a traditional Chinese medicine composition for later use;
(4) mixing the traditional Chinese medicine composition with each strain culture solution obtained in the step (2) according to the proportion of adding 30mL of strain culture solution into every 150g of the traditional Chinese medicine composition, and performing closed anaerobic fermentation at the fermentation temperature of 30-37 ℃ for 48-72 hours to obtain fermentation products of each strain for later use;
(5) and (3) fermenting products of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis obtained in the step (3) according to the viable bacteria number ratio of 0.5-1: 1-2: 0.5-2: 1-2: 0.5-2, uniformly stirring, and freeze-drying to finally obtain the fermented traditional Chinese medicine microecological preparation.
Applications Claiming Priority (2)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114984114A (en) * | 2022-06-06 | 2022-09-02 | 湖北武当动物药业有限责任公司 | Liquid fermentation traditional Chinese medicine microecological preparation and application thereof in aspect of resisting porcine epidemic diarrhea virus |
| CN115463190A (en) * | 2022-08-22 | 2022-12-13 | 山农农牧(泰安)有限公司 | Fermented traditional Chinese medicine preparation for preventing and treating diarrhea of fattening sheep and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113616715B (en) * | 2021-08-31 | 2022-05-06 | 昆明理工大学 | Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora thereof |
| CN115444876B (en) * | 2022-09-07 | 2024-02-02 | 华中农业大学 | Fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and preparation method thereof |
| CN116162557B (en) * | 2023-02-03 | 2023-09-01 | 大连三仪动物药品有限公司 | A strain of Saccharomyces cerevisiae, chinese medicinal microecological preparation for preventing and treating ruminant diarrhea, and its preparation method |
| CN116210820A (en) * | 2023-03-09 | 2023-06-06 | 河南亿万中原饲料有限公司 | Traditional Chinese medicine fermented feed for preventing and treating diarrhea of piglets and preparation method |
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