Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea and a preparation method thereof.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
on one hand, the invention provides a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, which comprises a traditional Chinese medicine composition and a compound microorganism; the traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of astragalus membranaceus crude extract, 5-10 parts of licorice root crude extract, 5-15 parts of madder root crude extract, 10-15 parts of purslane crude extract, 10-20 parts of fructus alpiniae oxyphyllae crude extract and 5-10 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5-1: 1-2: 0.5-2: 1-2: 0.5 to 2.
Further, as an optimization of a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, the traditional Chinese medicine composition comprises the following components in parts by weight: 14-18 parts of astragalus membranaceus crude extract, 5-8 parts of licorice root crude extract, 8-14 parts of madder root crude extract, 12-15 parts of purslane crude extract, 12-16 parts of fructus alpiniae oxyphyllae crude extract and 5-8 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5-0.8: 1.2-1.8: 0.8-1.6: 1.2-1.6: 0.8 to 1.6.
Further, as an optimization of a fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, the traditional Chinese medicine composition comprises the following components in parts by weight: 15 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 10 parts of madder crude extract, 15 parts of purslane crude extract, 14 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract; the compound microorganism comprises clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.6: 1.6: 1: 1.5: 1.2.
on the other hand, the invention also provides a preparation method of the fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea, which comprises the following steps:
(1) selecting genuine medicinal materials such as astragalus, liquorice, madder, purslane, fructus alpiniae oxyphyllae and poplar flower, respectively carrying out immersion extraction for 2 times, then carrying out spray drying on extract liquor, and sieving with a 200-mesh sieve for later use after crushing;
(2) clostridium butyricum, Bacillus subtilis, Lactobacillus acidophilus,Respectively inoculating the bacterial liquid of bacillus geotrichum and enterococcus faecalis into respective liquid culture medium in an inoculation amount of 2%, fermenting at 30-37 deg.C for 36-72 hr to obtain each strain culture liquid, wherein the viable bacteria number of each strain culture is 0.5 × 1010CFU/ml~1.0×1012CFU/ml;
(3) Accurately weighing the traditional Chinese medicine extracts in the step (1) according to the following parts by weight: 10-20 parts of astragalus membranaceus crude extract, 5-10 parts of licorice root crude extract, 5-15 parts of madder root crude extract, 10-15 parts of purslane crude extract, 10-20 parts of fructus alpiniae oxyphyllae crude extract and 5-10 parts of poplar flower crude extract, and uniformly mixing to obtain a traditional Chinese medicine composition for later use;
(3) mixing the traditional Chinese medicine composition with each strain culture solution obtained in the step (2) according to the proportion of adding 30mL of strain culture solution into every 150g of the traditional Chinese medicine composition, and performing closed anaerobic fermentation at the fermentation temperature of 30-37 ℃ for 48-72 hours to obtain fermentation products of each strain for later use;
(4) and (3) fermenting products of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis obtained in the step (3) according to the viable bacteria number ratio of 0.5-1: 1-2: 0.5-2: 1-2: 0.5-2, uniformly stirring, and freeze-drying to finally obtain the fermented traditional Chinese medicine microecological preparation.
(III) advantageous effects
The microbial ecological agent for treating piglet diarrhea provided by the invention is prepared by fermenting a traditional Chinese medicine composition and a compound microorganism by using a modern fermentation technology. The invention utilizes the characteristic that traditional Chinese medicines are not easy to generate drug resistance and drug residue of animal products, selects six traditional Chinese medicinal materials of astragalus, liquorice, madder, purslane, alpinia oxyphylla and poplar flower, and is prepared by scientifically mixing various microorganisms such as clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, bacillus geotrichum and enterococcus faecalis and fermenting the traditional Chinese medicines. According to the invention, different microorganisms are reasonably proportioned, so that the number of beneficial microorganisms in intestinal tracts of piglets can be increased, the intestinal mucosa can be repaired conveniently, and meanwhile, the effective components and active substances of traditional Chinese medicines can be extracted and utilized to the greatest extent through the fermentation of the composite microorganisms, so that the absorption rate of the piglets is increased. The invention can effectively prevent and treat diarrhea of piglets, remarkably reduce the death rate, has positive effect on weight increment of piglets and promotes the growth and development of piglets.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A fermented traditional Chinese medicine microecological preparation for preventing and treating piglet diarrhea is prepared according to the following steps:
(1) selecting genuine medicinal materials such as astragalus, liquorice, madder, purslane, fructus alpiniae oxyphyllae and poplar flower, respectively carrying out immersion extraction for 2 times, then carrying out spray drying on extract liquor, and sieving with a 200-mesh sieve for later use after crushing;
(2) respectively inoculating the bacterial liquid of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis into respective liquid culture medium by the inoculation amount of 2%, fermenting for 36-72 hours at 30-37 ℃ to obtain each strain culture liquid, wherein the viable bacteria number of each strain culture is 0.5 multiplied by 1010CFU/ml~1.0×1012CFU/ml;
(3) Accurately weighing the traditional Chinese medicine extracts in the step (1) according to the following parts by weight: 10 parts of astragalus membranaceus crude extract, 5 parts of licorice root crude extract, 5 parts of madder root crude extract, 10 parts of purslane crude extract, 10 parts of alpinia oxyphylla crude extract and 5 parts of poplar flower crude extract, and uniformly mixing to obtain a traditional Chinese medicine composition for later use;
(3) mixing the traditional Chinese medicine composition with each strain culture solution obtained in the step (2) according to the proportion of adding 30mL of strain culture solution into every 150g of the traditional Chinese medicine composition, and performing closed anaerobic fermentation at the fermentation temperature of 30-37 ℃ for 48-72 hours to obtain fermentation products of each strain for later use;
(4) and (3) fermenting products of clostridium butyricum, bacillus subtilis, acidophilic lactobacillus, geobacillus and enterococcus faecalis obtained in the step (3) according to the viable bacteria quantity ratio of 0.5: 1: 0.5: 1: 0.5, uniformly stirring, and freeze-drying to finally obtain the fermented traditional Chinese medicine microecological preparation.
Example 2
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20 parts of astragalus membranaceus crude extract, 10 parts of liquorice crude extract, 15 parts of madder crude extract, 15 parts of purslane crude extract, 20 parts of fructus alpiniae oxyphyllae crude extract and 10 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 1: 2: 2: 2: 2.
example 3
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 14 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 8 parts of madder crude extract, 12 parts of purslane crude extract, 12 parts of fructus alpiniae oxyphyllae crude extract and 5 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.5: 1.2: 0.8: 1.2: 0.8.
example 4
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 18 parts of radix astragali crude extract, 8 parts of licorice crude extract, 14 parts of madder crude extract, 15 parts of purslane crude extract, 16 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.8: 1.8: 1.6: 1.6: 1.6.
example 5
This example differs from example 1 in that:
the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 parts of astragalus membranaceus crude extract, 5 parts of liquorice crude extract, 10 parts of madder crude extract, 15 parts of purslane crude extract, 14 parts of fructus alpiniae oxyphyllae crude extract and 8 parts of poplar flower crude extract;
the compound microorganism comprises clostridium butyricum, bacillus subtilis, lactobacillus acidophilus, bacillus geotrichum and enterococcus faecalis, and the number ratio of viable bacteria is 0.6: 1.6: 1: 1.5: 1.2.
in order to further illustrate the treatment effect of the fermented traditional Chinese medicine microecological preparation on piglet diarrhea, an animal experiment is provided as an illustration below; the fermented traditional Chinese medicine microecologics mentioned below were prepared according to example 5.
1 materials and methods
1.1 test drugs, test animals
Test drugs: the fermented traditional Chinese medicine micro-ecological preparation prepared in example 5, florfenicol soluble powder.
Test animals: 60 weaned piglets suffering from diarrhea and about 20 days old are selected from the pig group.
1.2 test methods
Randomly dividing 60 weaned piglets suffering from diarrhea into 3 groups, wherein each group comprises 20 piglets, namely a blank group, a positive control group and a test group; healthy and disease-free 20 piglets are taken as a prevention group; the blank group is administrated with 2mL of physiological saline per kilogram of piglet weight; the positive control group is administrated with florfenicol soluble powder 15mg per kilogram of piglet weight; the experimental group is administrated with 15mg of the fermented traditional Chinese medicine microecological preparation per kilogram of piglet weight; during the test period, all the test piglets are raised under the same condition, are fed freely, drink freely and are fed with less feeding; the mental, fecal and appetite status and death status of the piglets are observed every day, the treatment process is carried out until the piglets are cured or die, and the test is also terminated if the piglets are not cured or die within one week.
1.3 therapeutic efficacy determination
The method has the following advantages: the shape of the excrement is improved, the diarrhea frequency is reduced, and the food intake is increased;
and (3) curing: the food intake is normal, the mental state is good, and the diarrhea symptom disappears;
and (4) invalidation: food intake and diarrhea symptoms did not improve, and diarrhea symptoms even worsened, or died.
2 results
Table 2 shows the treatment results of the piglets of each group during the trial. As can be seen from the results in Table 2, the mortality rate of piglets suffering from diarrhea is as high as 65% without drug treatment, which indicates that the diarrhea of piglets has serious harm to the growth of piglets; compared with the blank group, after the piglets are treated, the florfenicol soluble powder and the fermented traditional Chinese medicine microecological preparation have obvious treatment effect on the diarrhea of the piglets, and the effective rate can reach 85 percent; compared with a positive control group, the effective rate of the test group on the treatment of the piglet diarrhea can reach 100 percent, is obviously higher than that of the positive control group, the cure rate can reach 90 percent, is also obviously higher than that of the positive control group, and meanwhile, the test group has no dead piglet cases. The test results show that the treatment effect of the fermented traditional Chinese medicine microecological preparation for treating the diarrhea of the piglets is better than the effective rate and the cure rate of the florfenicol soluble powder for treating the diarrhea of the piglets, the death of the piglets caused by the diarrhea can be effectively avoided, and the fermented traditional Chinese medicine microecological preparation can replace antibiotics to treat the diarrhea of the piglets.
TABLE 2
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.