CN113925894A - Antibacterial and anti-inflammatory Chinese herbal medicine extract feed additive and application thereof - Google Patents
Antibacterial and anti-inflammatory Chinese herbal medicine extract feed additive and application thereof Download PDFInfo
- Publication number
- CN113925894A CN113925894A CN202111266262.9A CN202111266262A CN113925894A CN 113925894 A CN113925894 A CN 113925894A CN 202111266262 A CN202111266262 A CN 202111266262A CN 113925894 A CN113925894 A CN 113925894A
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- extract
- chinese herbal
- herbal medicine
- feed additive
- feed
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Abstract
The invention relates to a Chinese herbal medicine extract feed additive with antibacterial and anti-inflammatory functions and application thereof, wherein the Chinese herbal medicine extract feed additive comprises a lonicera confusa extract, a scutellaria baicalensis extract and a fermented traditional Chinese medicine compound, and the mass ratio of the lonicera confusa extract to the scutellaria baicalensis extract is 1: 1-6. The Chinese herbal medicine extract provided by the invention has the advantages of obvious antibacterial and anti-inflammatory effects, low cost and difficulty in generating drug resistance. The application value of the Chinese herbal medicine extract additive in the feed is determined through animal experiments, a new application path is opened for the application of the Chinese herbal medicine extract additive in the feed, and a direction for reducing cost and improving efficiency is provided for feed enterprises.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a Chinese herbal medicine extract feed additive with antibacterial and anti-inflammatory effects and application thereof.
Background
In recent years, the domestic feed processing industry, the breeding industry and the like are vigorously developed, the feed raw material demand is greatly increased, the corn and the soybean meal produced locally are difficult to meet the market demand, so that the market prices of the corn and the soybean meal are continuously increased in recent years, and the price of other feed raw materials is driven to be increased, so that the feed price is greatly increased; on the other hand, since the feed end is forbidden to resist the culture end and reduce resistance in 7 months in 2020, problems of frequent culture site, especially diarrhea caused by harmful microorganism infection, low growth efficiency, high culture cost and the like, and a feed factory faces double pressure. Therefore, the development of the green feed additive with the effects of bacteriostasis, inflammation diminishing and growth promotion is an important measure for guaranteeing healthy culture and improving culture benefits in the post-antibiotic age.
The Chinese herbal medicine extract is derived from the application basis of 5000 years of traditional treasure Chinese herbal medicines in China, is processed by a modern processing technology, has multiple effects of resisting bacteria, viruses, inflammation and immunity according to the traditional Chinese medicine, can prevent and treat diseases, can improve the production performance, does not generate drug resistance, does not have phytotoxicity residue in livestock and poultry products, and is a green feed additive for ensuring the health of animals in the case of post-antibiotics.
Chinese herbal medicine extract products in the market are uneven at present, are designed according to the theories of 'monarch prime, adjuvant prime, Yin-Yang complementation' and the like in the traditional Chinese medicine, have the effects of clearing heat, removing toxicity, resisting inflammation, diminishing inflammation, improving immunity and the like, are mainly used as veterinary medicine preparations for preventing and treating diseases on a culture site, and have limited application range; in addition, the product has complex components, unclear content of active ingredients, difficult quality control and influence on use stability and effectiveness. In order to promote the wide application of the Chinese herbal medicine extract in the livestock and poultry feed and solve the problem of the application of the Chinese herbal medicine extract, a plurality of experts propose the Chinese herbal medicine extract to determine the content and the function of effective components according to the idea of traditional Chinese medicine westernization, combine the function with the practical application of animal nutrition, especially improve the digestibility of nutrient substances, promote the growth of animals, really play the application value of the Chinese herbal medicine extract in the feed, and reduce the cost and improve the efficiency for feed enterprises.
Disclosure of Invention
The invention aims to provide a Chinese herbal medicine extract feed additive for bacteriostasis and inflammation diminishing, which comprises a lonicera confusa extract, a scutellaria baicalensis extract and a composite fermentation traditional Chinese medicine;
wherein the mass ratio of the lonicera confusa extract to the scutellaria baicalensis extract is 1: 1-6.
The feed additive provided by the invention has obvious antibacterial and anti-inflammatory effects, is low in cost, is not easy to generate drug resistance, can effectively prevent harmful pathogenic microorganism infection diseases such as diarrhea or necrotic enteritis and the like at the breeding end after the feed is forbidden to resist, and can improve the intestinal health of animals.
The Lonicera confusa is dried bud or flower of Lonicera macranthoides, Lonicera hypoglauca, Lonicera confusa or Lonicera flavipes of Caprifoliaceae. The genuine areas of the lonicera confusa in China are mainly distributed in southern areas such as Hunan, Sichuan and the like, and have the effects of clearing away heat and toxic materials and dispelling wind and heat. The flos Lonicerae is rich in organic acids and saponins, wherein the chlorogenic acid of organic acids is an active ingredient with antibacterial, antiviral, antiinflammatory, antioxidant, liver protecting and gallbladder promoting effects, and is also one of quality control ingredients of flos Lonicerae effective components in Chinese pharmacopoeia. Although many Chinese herbal medicine plants contain chlorogenic acid, the biological activity of the chlorogenic acid is closely related to the variety and the producing area of the Chinese herbal medicine, and the biological activity of the chlorogenic acid in the lonicera confusa in the producing area of the genuine land is the best. Preferably, the content of chlorogenic acid in the flos Lonicerae extract is 5% -10%.
The scutellaria baicalensis is a dry root of scutellaria baicalensis of a labiate family, is abundant in resources of scutellaria baicalensis in China, is mainly produced in Hebei, Henan, Shandong, Shanxi and inner Mongolia, is located in Chengdu city and Shandong Juxian county of Hebei province, is bitter in taste and cold in nature, and has the effects of clearing heat and drying dampness, purging fire and detoxifying, stopping bleeding and preventing miscarriage. The scutellaria baicalensis is rich in flavonoid components, and also contains volatile oil, sterol, alkaloid and the like, but the flavonoid compound baicalin with the most varieties and the most obvious biological activity is also a quality control component of the scutellaria baicalensis effective components in Chinese pharmacopoeia. Baicalin activity is also affected by the producing area, and baicalin biological activity in the producing area is the best. Preferably, the baicalin content in Scutellariae radix extract is 50-80%.
The composite Chinese herbal medicine extract provided by the invention takes the lonicera confusa extract and the scutellaria baicalensis extract as main bodies, the chlorogenic acid and the baicalin which are effective components in the two extracts have certain antibacterial, antiviral, endotoxin-resisting and anti-inflammatory effects, and the chlorogenic acid and the baicalin have certain synergistic effects on the aspects of bacteriostasis, inflammation resistance, endotoxin resistance and prevention and treatment of intestinal diseases. In order to maximize the synergistic effect of the two traditional Chinese medicine extracts on the aspects of bacteriostasis and inflammation diminishing, the invention further optimizes the mixture ratio and screens the optimal mixture ratio range. When the ratio of the honeysuckle extract to the scutellaria extract is in a range of 1:1-4, the honeysuckle extract and the scutellaria extract have broad-spectrum antibacterial effect, and particularly, the mass ratio of the lonicera confuse extract to the scutellaria extract is 1: 2-4.
The lonicera confusa extract and the scutellaria baicalensis extract provided by the invention are extracted by an ultrasonic water extraction method, single Chinese herbal medicine extract powder is prepared by the technologies of single raw material ultrasonic negative pressure extraction, online ultrahigh speed centrifugation, negative pressure low temperature concentration, vacuum freeze drying or spray drying and the like, and then the composite Chinese herbal medicine extract feed additive is prepared by refining according to a certain proportion.
The lonicera confusa extract is prepared from lonicera confusa in a genuine producing area and the scutellaria baicalensis extract is prepared from scutellaria baicalensis in the genuine producing area.
The method comprises the following specific steps:
the lonicera confusa extract is prepared by the following steps: mixing flos Lonicerae with water according to a material-liquid ratio of 1:10-1: 15; extracting at 50-80 deg.C for 3-4 hr for 2-4 times to obtain flos Lonicerae extract; then collecting filtrate by ultra-high speed centrifugation, and performing low-temperature vacuum concentration and spray drying to obtain flos Lonicerae extract dry powder with effective component content 5-10% calculated by chlorogenic acid.
The scutellaria baicalensis extract is prepared by adopting the following specific method: cutting Scutellariae radix into 2-4cm, mixing with water at a ratio of 1:10-1:20, extracting at 50-80 deg.C for 2-3 hr for 3-4 times to obtain Scutellariae radix extractive solution, centrifuging at ultra high speed, collecting filtrate, vacuum concentrating at low temperature, and spray drying to obtain Scutellariae radix extract dry powder with effective component content of 50-80% calculated by baicalin.
Wherein, the feed-liquid ratio is the conventional solid-liquid ratio, and the unit is mg/mL, kg/L and the like.
The preparation method is simple to operate, ensures the stability of the feed additive, and improves the application stability and uniformity of the final product.
The invention also comprises a compound fermentation traditional Chinese medicine in order to increase the synergistic effect of the lonicera confusa extract and the scutellaria baicalensis extract. The compound fermentation traditional Chinese medicine can enhance the application effect of the product from two aspects, namely, the traditional Chinese medicine can complement the cold and warm from the perspective of the traditional Chinese medicine, mutually promote the cold and warm, inhibit bacteria and diminish inflammation, improve the immunity and treat both symptoms and root causes; the auxiliary materials contain abundant probiotics such as lactobacillus, bacillus and microzyme, and can effectively adjust the intestinal microecological balance; meanwhile, the produced metabolites, small peptides, organic acids and enzyme preparations can improve the palatability of the product, promote the digestion and absorption of three nutrient substances and improve the feed conversion rate.
Wherein, the compound fermentation traditional Chinese medicine takes two or more of astragalus, liquorice, bran, corn and bean pulp as substrates;
wherein the probiotics in the compound fermentation traditional Chinese medicine is 10-50 hundred million cfu/g;
wherein the mass ratio of the lonicera confusa extract to the composite fermented traditional Chinese medicine is 1: 2-6; preferably 1: 4-6.
The compound fermentation traditional Chinese medicine is prepared by adopting the following method: taking two or more of astragalus, liquorice, bran, corn and bean pulp as substrates, inoculating a composite fermentation strain, piling and fermenting for 3-5 days in a fermentation tank under the conditions that the water content is 33-42% and the temperature is 25-40 ℃, and then drying by a roller dryer to prepare the compound fermentation strain; preferably, astragalus, liquorice, bran and soybean meal are used as substrates, 2-4 per mill of fermentation strains are inoculated according to the dry basis weight ratio, the accumulated fermentation is carried out in a tank type fermentation tank under the conditions that the water content is 38 percent and the temperature is 35-40 ℃, the fermentation time is 3 days, and then the fermentation is carried out by drying, wherein the beneficial microorganism content is 10-50 hundred million cfu/g; preferably 20-30 hundred million cfu/g.
Wherein the composite fermentation strain is two or more of lactobacillus, yeast, protease and cellulase; preferably, the inoculation is carried out in a weight ratio of 2 to 4 per thousand.
The Chinese herbal medicine extract provided according to the technical mode has obvious bacteriostatic function and anti-inflammatory functions, and can effectively inhibit the growth of gram-positive bacteria and gram-negative bacteria as shown in the following specific embodiment (see figure 1); meanwhile, for necrotic enteritis caused by clostridium perfringens, intestinal mucosa injury caused by clostridium perfringens after toxin attack can be quickly repaired, and intestinal inflammatory factor storm is reduced (see figure 2); meanwhile, the Chinese herbal medicine extract additive is applied to livestock and poultry, so that the loss of nutrient substances in inflammatory reaction is reduced, the digestion and absorption of the nutrient substances are promoted, the feed conversion rate is improved, the growth of animals is promoted, and the economic benefit is improved.
The invention further provides a preferable scheme, wherein the Chinese herbal medicine extract feed additive comprises a lonicera confusa extract, a scutellaria baicalensis extract and a compound fermentation traditional Chinese medicine in a mass ratio of 1:2-4: 4-6;
wherein the content of chlorogenic acid as an effective component in the lonicera confusa extract is 5-10%;
the content of effective component baicalin in the Scutellariae radix extract is 50-80%;
the compound fermentation traditional Chinese medicine is obtained by fermenting two or more of astragalus, liquorice, bran, corn and bean pulp serving as substrates; the probiotics accounts for 20-30 hundred million cfu/g;
preferably, the Chinese herbal medicine extract feed additive is prepared by adopting the following method:
(1) extracting the lonicera confusa extract: mixing flos Lonicerae with water at a ratio of 1:10-1:15 by ultrasonic water extraction method, extracting at 50-80 deg.C for 3-4 hr for 2-4 times to obtain flos Lonicerae extract, centrifuging at ultra high speed, collecting filtrate, vacuum concentrating at low temperature, and spray drying to obtain flos Lonicerae extract dry powder with effective component content of 5-10% calculated as chlorogenic acid.
(2) Extracting the scutellaria baicalensis extract: taking radix Scutellariae of genuine producing area as raw material, cutting radix Scutellariae into 2-4cm length by ultrasonic water extraction method, mixing with water at a ratio of material to liquid of 1:10-1:20, extracting at 50-80 deg.C for 3-4 times (2-3 hr each time) to obtain radix Scutellariae extractive solution, centrifuging at ultra high speed to collect filtrate, vacuum concentrating at low temperature, and spray drying to obtain radix Scutellariae extract dry powder with effective component content of 50-80% calculated by baicalin.
(3) Fermentation of the compound fermented traditional Chinese medicine: two or more of astragalus, liquorice, bran, corn and bean pulp are used as substrates, a compound fermentation strain is inoculated, the mixture is piled and fermented in a fermentation tank for 3 to 5 days under the conditions that the water content is 33 to 42 percent and the temperature is 25 to 40 ℃, and then the mixture is dried by a roller dryer to prepare the microbial fertilizer, wherein the content of beneficial microorganisms is 20 to 30 hundred million cfu/g.
(4) And (3) physically mixing the prepared lonicera confusa extract, the scutellaria baicalensis extract and the compound fermented traditional Chinese medicine according to a ratio to obtain the Chinese herbal medicine extract feed additive.
The invention also aims to provide the application of the Chinese herbal medicine extract feed additive in broiler feed; in particular to the application on white feather broiler feed and yellow feather broiler feed. The application value of the Chinese herbal medicine extract in the feed is determined through animal experiments, a new application path is opened for the application of the Chinese herbal medicine extract in the feed, and a direction for reducing cost and improving efficiency is provided for feed enterprises.
Wherein the addition amount of the Chinese herbal medicine extract feed additive in the basic daily ration is 200-1000 mg/kg; preferably 200 to 500 mg/kg.
In the basic ration containing the Chinese herbal medicine extract feed additive, when the Chinese herbal medicine extract feed additive is 250-500 mg/kg, particularly when the addition amount is 280-320 mg/kg, the growth of animals can be promoted, the average weight of the fed animals is 75-130g higher than that of a control group, the feed conversion rate is improved, the feed-meat ratio is reduced by 0.03-0.05, and the input-output ratio is optimal as shown in the following specific implementation mode (see table 4, table 5 and table 8). Meanwhile, under the large background of high price of feed raw materials, the Chinese herbal medicine extract can save nutrient substances consumed by inflammatory reaction in animal organisms through bacteriostasis and anti-inflammation, and nutrient elements with corresponding content can be directly reduced when the nutrient concentration of the daily ration formula is set, so that cost reduction and efficiency improvement of feed enterprises are realized. In the concrete aspect, the additive can save the metabolic energy of the formula of 30-100 Mcal/ton broiler compound feed, reduce the addition of oil in the feed and the use amount of soybean meal in the feed, and the formula space is replaced by corn to ensure that the protein content and the amino acid level in daily ration are kept unchanged, so that the formula cost is finally reduced by 30-50 yuan, and meanwhile, the additive has an obvious growth promoting effect and achieves the effects of obviously reducing cost and improving efficiency (see table 8).
The Chinese herbal medicine extract feed additive provided by the invention has a particularly remarkable bacteriostatic and bactericidal effect, and can be used for adjusting the intestinal health of broiler chickens; in practical application, the anti-inflammatory and anti-inflammatory effects can save energy consumed by inflammatory reaction of organisms, reduce metabolic energy in a feed formula and reduce feed cost; thereby promoting the digestion and absorption of nutrient substances and improving the growth performance of animals; further improving the health state of animals and increasing the survival rate; the economic benefit of the cultivation is improved to the maximum extent.
In conclusion, the Chinese herbal medicine extract feed additive provided by the invention has the following specific beneficial effects:
1. the feed additive has obvious bacteriostatic and anti-inflammatory effects, is low in cost and not easy to generate drug resistance, can effectively prevent the feed from inhibiting harmful pathogenic microorganism infection diseases such as diarrhea or necrotic enteritis and the like at the breeding end after forbidden resistance, and improves the intestinal health of animals.
2. The Chinese herbal medicine extracts are prepared by taking genuine medicinal materials as raw materials, performing single extraction through a safe extraction process, and then compounding according to a certain proportion, and are fixed in components and stable in content, so that the application stability of the product can be improved;
3. the Chinese herbal medicine extract is organically combined with the fermented traditional Chinese medicine, has the effects of temperature and cold complementation, bacteriostasis, inflammation diminishing, immunity enhancing and intestinal tract regulating interaction, mutually promotes and treats both principal and secondary aspects of diseases, can quickly eliminate inflammatory states of animal organisms caused by various stresses, ensures animal health, reduces the medication cost of a culture site, promotes digestion and absorption of nutrient substances, promotes animal growth, improves the feed conversion rate and improves the culture economic benefit.
4. Under the background of price rise of feed raw materials, the Chinese herbal medicine extract can save the consumption of nutrient elements caused by inflammation through bacteriostasis and inflammation diminishing, and corresponding nutrient elements can be directly reduced when the nutrient concentration of the daily ration formula is set, so that cost reduction and efficiency improvement are realized.
Drawings
FIG. 1 is a graph of the bacteriostatic effect of the Oxford cup method provided in Experimental example 2;
FIG. 2 is a graph comparing the staining results of ileum histopathological sections in Experimental example 3;
FIG. 3 is a graph comparing the amounts of inflammatory factors in ileal tissue in Experimental example 3.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The lonicera confusa extract, the scutellaria baicalensis extract and the compound fermentation traditional Chinese medicine in the following embodiments are prepared by the following method:
(1) preparing the lonicera confusa extract: selecting and using the lonicera confusa of Hunan Longhui, removing impurities, adding 10-12 times of distilled water, soaking for 1h, performing ultrasonic extraction at the temperature of 50-70 ℃, extracting for 2-3 times, each time for 2-3h, combining the extracting solution of each time to obtain the lonicera confusa extracting solution, then performing high-speed centrifugation at 8000-10000rpm, collecting filtrate, performing reduced pressure concentration at the temperature of 40-50 ℃, and performing freeze drying to obtain a powdery lonicera confusa extract, wherein the content of effective components is 5-10% by weight of chlorogenic acid.
(2) Preparing a scutellaria baicalensis extract: selecting Hebei Chengde radix Scutellariae, removing impurities, cutting into 2-3cm length, adding 9-12 times of distilled water, soaking for 2-3h, ultrasonic extracting at 50-80 deg.C for 3-5 times, each for 3-4h, mixing extractive solutions to obtain flos Lonicerae extract, centrifuging at 8000-12000rpm, collecting filtrate, concentrating under reduced pressure at 40-50 deg.C, and spray drying to obtain powdery radix Scutellariae extract with effective component content of 50-80% calculated by baicalin.
(3) The compound fermentation traditional Chinese medicine is prepared by taking astragalus, liquorice, bran and corn as substrates, inoculating 2-3 per mill of fermentation strain compounded by lactic acid bacteria, saccharomycetes, protease and cellulase according to the weight ratio, accumulating and fermenting for 3-5 days in a fermentation tank under the conditions that the water content is 38-40% and the temperature is 35-40 ℃, and drying by a drum dryer, wherein the content of beneficial microorganisms is 20-30 hundred million cfu/g.
The flower extract, the scutellaria baicalensis extract and the compound fermented traditional Chinese medicine obtained by the method are physically mixed and uniformly mixed according to the actual proportion.
Example 1
The embodiment provides a Chinese herbal medicine extract feed additive for inhibiting bacteria and diminishing inflammation; the composition comprises the following components in parts by weight: 1 part of lonicera confusa extract (with chlorogenic acid content of 8-10%), 5 parts of scutellaria baicalensis extract (with baicalin content of 20-30%) and 4 parts of composite fermentation traditional Chinese medicine (with beneficial bacterium content of 20-30 hundred million cfu/g).
Example 2
The embodiment provides a Chinese herbal medicine extract feed additive with antibacterial and anti-inflammatory effects, which is composed of the following components in parts by weight: 1 part of lonicera confusa extract (with chlorogenic acid content of 8-10%), 3 parts of scutellaria baicalensis extract (with baicalin content of 50-80%) and 6 parts of composite fermentation traditional Chinese medicine (with beneficial bacterium content of 20-30 hundred million cfu/g).
Example 3
The embodiment provides a Chinese herbal medicine extract feed additive with antibacterial and anti-inflammatory effects, which is composed of the following components in parts by weight: 1 part of lonicera confusa extract (with chlorogenic acid content of 8-10%), 4 parts of scutellaria baicalensis extract (with baicalin content of 50-80%) and 5 parts of composite fermentation traditional Chinese medicine (with beneficial bacterium content of 20-30 hundred million cfu/g).
Example 4
The embodiment provides a Chinese herbal medicine extract feed additive with antibacterial and anti-inflammatory effects, which is composed of the following components in parts by weight: 2 parts of flos lonicerae extract (chlorogenic acid content is 8-10%), 4 parts of radix scutellariae extract (baicalin content is 50-80%) and 4 parts of composite fermentation traditional Chinese medicine (beneficial bacterium content is 20-30 hundred million cfu/g).
Example 5
The embodiment provides a Chinese herbal medicine extract feed additive for bacteriostasis and inflammation diminishing, which is different from the embodiment 2 only in that the mass ratio of the lonicera confusa extract to the scutellaria baicalensis extract is 1: 1.
comparative examples 1 to 3
The embodiment provides a Chinese herbal medicine extract for bacteriostasis and inflammation diminishing, which is different from the embodiment 2 only in that the mass ratio of the lonicera confusa extract to the scutellaria baicalensis extract is 2: 1; 3: 1; 4: 1.
experimental example 1
The inhibition effects of examples 2 to 3 and 5 and comparative examples 1 to 3 on harmful bacteria were compared, specifically as follows:
TABLE 1 inhibitory Effect of various proportions of the extracts of lonicera confusa and scutellaria baicalensis on harmful bacteria
TABLE 2 Effect of the composition of the Lonicera confusa extract and Scutellaria baicalensis extract at different ratios on the intestinal inflammatory factors of ulcerative colitis in mice
Experimental example 2
The antibacterial property of the prepared Chinese herbal medicine extract feed additive is evaluated by an oxford cup method.
Test materials: using the herbal extract feed additives prepared in examples 1 and 2; an oxford cup; LB solid culture medium; a flat plate; tweezers; escherichia coli, Staphylococcus aureus, LB liquid medium; sterilizing water; cotton swab
The test steps are as follows:
(1) sample pretreatment: mixing and diluting the Chinese herbal medicine extract with 40-60% methanol solution according to the weight ratio of 1:10, extracting for 1-2h at room temperature by shaking, and taking supernatant for later use.
(2) Preparing an indicator bacterium: inoculating Escherichia coli and Staphylococcus aureus to LB liquid culture medium, culturing at 37 deg.C in constant temperature incubator for 12-18h, and diluting to 2-5 × 107cfu/ml for use.
(3) And (3) oxford cup method operation: and uniformly coating 120ml of the prepared indicator bacterium liquid on a sterilized LB solid culture plate, standing the Oxford cup on the solid plate after the bacterium liquid on the plate is dried, adding 200 microliters of the extraction solution (a control group) and the plant extract extraction supernatant into the Oxford cup respectively, culturing at 37 ℃ for 12-15h, and observing and recording the size of the inhibition zone.
The test results are shown in figure 1 (bacteriostatic effect diagram) and table 3 below. As can be seen from FIG. 1 and Table 3, the Chinese herbal medicine plant extract feed additives of examples 1 and 2 have bacteriostatic effects on Escherichia coli and Staphylococcus aureus, wherein the bacteriostatic effect on Staphylococcus aureus is superior to that of Escherichia coli. Can effectively inhibit the growth of gram-negative bacteria (taking escherichia coli as an example) and gram-positive bacteria (taking staphylococcus aureus as an example).
TABLE 3 bacteriostatic effect of Chinese herbal medicine extract
Experimental example 3
The Chinese herbal medicine extract feed additive provided in the embodiment 2 is added into basic daily ration of broiler feed, and the addition amount is 500 mg/kg; observing the prevention and treatment effect of the strain on clostridium perfringens infection in the production of broiler chickens.
90 AA broiler chickens of 1 day age are selected in the test, and are randomly divided into 3 groups, namely a blank control group M (basic ration, not attacked by poison) and a positive control group A (basic diet, attacked by poison); test group B (basal diet + herbal extract feed additive 500mg/kg, challenge), normal feeding management.
In the test, at the age of 14-17 days, the group A and the group B chickens are continuously subjected to intragastric administration of clostridium perfringens bacterial liquid for 3 days, the blank control group M is subjected to intragastric administration of normal saline, the meat chickens are taken from intestinal tract tissues 1d before challenge, 1d after challenge and 5d after challenge to observe the pathological changes of intestinal villi and the structural changes of the intestinal villi, and the expression of inflammation related factors in the intestinal tract tissues is measured.
The staining results of the ileal histopathological sections are shown in fig. 2, wherein a: a positive control group; b: chinese herbal medicine extract group. As can be seen from FIG. 2, after the clostridium perfringens attacks the toxin, the ileum villi in the group A is broken, the structure of the ileum villi becomes loose and expands, and the breakage condition becomes more obvious along with the prolonging of the attacking time, so that the ileum morphological injury and acute inflammation caused by the clostridium infection can be seen. After the Chinese herbal medicine extract is added into the group B, the toxin is attacked by the clostridium perfringens, partial breakage of intestinal villi can be seen in 1d after the toxin attack, compared with the group A, the intestinal villi is slight, and the intestinal villi gradually recovers to a normal state along with the prolonging of the action time, which shows that the Chinese herbal medicine extract has a good protection effect on necrotic enteritis caused by the clostridium perfringens and can quickly repair the abnormal state of the intestinal villi.
Wherein the ileum tissue inflammatory factor content is shown in figure 3 (ileum tissue inflammatory factor content change after clostridium perfringens challenge, wherein M is a blank control group, A is a positive control group, and B is a Chinese herbal medicine extract group). Compared with the blank control group M, the ileum tissue inflammatory factor expression levels of the positive control group A and the Chinese herbal medicine extract group B are increased to different degrees 1d after the clostridium perfringens is attacked, and the fact that the clostridium perfringens can cause different degrees of inflammatory storms in intestinal tracts after being infected is proved. Compared with the group A, the Chinese herbal medicine extract added into the feed in the group B remarkably reduces the content of IL-16, IL-17, IL-22, IFN-alpha and IFN-gamma inflammatory factors (p is less than 0.01), so that the Chinese herbal medicine extract has obvious anti-inflammatory and anti-inflammatory effects and can protect intestinal tissues from inflammatory storm caused by clostridium perfringens.
Experimental example 4
The Chinese herbal medicine extract feed additive provided in the embodiment 2 is added into basic ration of broiler feed, and the Chinese herbal medicine extract feed additive is added into the broiler feed at a ratio of 300 mg/kg.
480 healthy 1-day-old AA broiler chickens with basically consistent weights were selected and randomly divided into 2 treatments, each treatment was 8 replicates, and each replicate was 30 chickens. The 2 treatments are respectively a control group and a Chinese herbal medicine extract group, the control group is fed with corn bean pulp type basic ration, and the Chinese herbal medicine extract group is obtained by adding 300g/t Chinese herbal medicine extract into the basic ration. And (5) performing conventional breeding management. The growth performance results are shown in table 4 below.
As can be seen from Table 4, compared with the control group, the daily food consumption of the group added with the herbal extracts at the early stage, the later stage and the full stage is 5.34%, 4.73% and 4.89%, the daily average weight gain is respectively increased by 6.16%, 7.59% and 7.0%, and the material ratio is respectively reduced by 0.02, 0.04 and 0.03. The death and elutriation rate in the whole period is reduced by 0.53 percent, compared with 63.86 percent, the feed conversion rate is improved by 2 percent.
And (3) economic benefit analysis:
from the perspective of breeding income, 127g of meat is grown from each chicken to the slaughter, the income is about 1.3 yuan, 100g of materials are consumed, 0.35 yuan is added, the cost of Chinese herbal medicine extract added into the feed is 0.05-0.06 yuan, and the profit of one chicken is about 0.9 yuan on average;
from the perspective of feed utilization rate, the conversion rate of the Chinese herbal medicine extract during the whole period is improved by 2 percent, the feed value is improved by 3500 x 2 percent to 70 yuan, the input cost is 20 to 25 yuan, and the creation value is 45 to 50 yuan.
TABLE 4 influence of herbal extracts on broiler growth Performance
Experimental example 5
The Chinese herbal medicine extract feed additive provided in the embodiment 2 is added into basic daily ration of broiler feed, and 250mg/kg, 500mg/kg and 1000mg/kg of the Chinese herbal medicine extract are added into the broiler feed.
1200 healthy 1-day-old AA broiler chickens with substantially consistent body weight were selected and randomly divided into 4 treatments, each treatment was 10 replicates, and each replicate was 30 chickens. The 4 treatments are respectively a control group, a 250g/t Chinese herbal medicine extract group, a 500g/t Chinese herbal medicine extract group and a 1000g/t Chinese herbal medicine extract group, the control group is fed with corn bean pulp type basic ration, and the Chinese herbal medicine extract group is formed by adding Chinese herbal medicine extracts with different dosages in the basic ration. And (5) performing conventional breeding management. The growth performance results are shown in table 5 below.
As can be seen from Table 5, compared with the blank control group, the group added with Chinese herbal medicine extracts with different doses in the daily ration of the broiler can reduce the material weight ratio of the early stage (1-21d), the later stage (22-42d) and the full stage (1-42d), wherein the group added with 250g of organic sugar is obviously different from the blank control group; meanwhile, the Chinese herbal medicine extracts with different doses tend to improve the growth of the broilers, and the weight of the slaughtered Chinese herbal medicine is improved by 62-88 g compared with that of a control group.
TABLE 5 influence of herbal extracts on broiler growth Performance
The immune function of the body is closely related to the development of immune organs, and the thymus, the bursa of fabricius and the spleen are important immune organs in the body of the broiler. The effect of herbal extracts on the development of immune organs of broilers is shown in table 6 below. As can be seen from table 6, at 21 days of age, the Chinese herbal medicine extract groups with different dosages all have a tendency to improve the thymus index, spleen index and bursal index of broilers, but the differences are not significant; at 42 days of age, the Chinese herbal medicine extract groups with different dosages can obviously improve the thymus index and the bursal disease index (p is less than 0.05) of the broiler chicken, and the spleen index is also improved, and the difference is not obvious. The Chinese herbal medicine extracts with different dosages show a dosage effect relationship on the improvement of immune organ indexes, and the higher the addition amount is, the better the effect is.
TABLE 6 influence of herbal extracts on immune organ index of broiler chickens
The Chinese herbal medicine extract feed additive has the effects of promoting the growth of broiler chickens, improving the feed conversion rate, promoting the development of immune organs of the broiler chickens and improving the disease resistance. By integrating the growth performance and the index of immune organs, the optimal addition amount of the Chinese herbal medicine extract feed additive is 250-500 g/t.
Experimental example 6
Under the background of high price of the current raw materials and high cost pressure of the feed, the Chinese herbal medicine extract is anti-inflammatory and anti-inflammatory, saves the energy consumed by animal organisms due to inflammation, reduces the energy of the feed formula, and finally achieves the application scheme of cost reduction and efficiency improvement.
960 healthy 1-day-old AA broilers of substantially uniform weight were selected and randomly divided into 4 treatments of 8 replicates each of 60 chickens. The experiment adopts a 2X 2 factorial design: i.e. feeding (normal energy ration VS energy decrement ration); the type of ration was corn, wheat, soybean meal, and the energy levels of the two rations are shown in table 7 below. On the basis of conventional energy daily ration and decrement daily ration, 0mg/kg or 300mg/kg of Chinese herbal medicine extract is respectively added. And (5) performing conventional breeding management.
Table 7 test ration energy design
Type of ration | Early formula metabolic energy (Mcal/t) | Late stageFormula metabolic energy (Mcal/t) |
Normal energy daily ration | 2950 | 3150 |
Daily ration with reduced energy | 2900 | 3100 |
The production performance of commercial broilers is shown in table 8, except that 300mg/kg of the Chinese herbal medicine extract feed additive is added to the commercial broilers respectively at two daily ration levels. As shown by analysis of variance, compared with normal energy daily ration, the energy-reducing daily ration group has the advantages that the weight ratio of materials in the range of 22-42d and 1-42d is remarkably reduced (P is less than 0.05), and the near-remarkable influence is exerted on the total-period daily gain and the weight average at the end of 42d (P is 0.052); compared with the group added with 0mg/kg of Chinese herbal medicine extract, the group added with 300mg/kg of Chinese herbal medicine extract can obviously reduce the feed weight ratio (p is less than 0.05) of 1-21d, 22-42d and 1-42d of the broiler chickens, and can obviously improve the daily gain (p is less than 0.05) of 1-42d and the weight (p is less than 0.05) of 42d chickens. Therefore, the growth performance is influenced and the feed ratio is increased by reducing the metabolic energy of 50Mcal of the commercial broiler ration; the addition of Chinese herbal medicine extract in daily ration can increase daily gain and reduce material weight ratio.
TABLE 8 influence of the addition of herbal extracts on the growth performance of broiler chickens under different daily ration conditions
The influence of the addition of 300mg/kg of the Chinese herbal medicine extract feed additive to commercial broilers at the level of two daily rations on the digestibility of the nutrient substances of the broilers is shown in Table 9. According to analysis of variance, compared with normal energy daily ration, the digestibility of DM and OM in the energy-reducing daily ration is respectively and complementarily improved by 2.08 percent and 2.45 percent, the digestibility of EE and GE is reduced by 0.64 percent and 0.36 percent, and the ADG is measured to be reduced from 3082 to 3071 Kcal/kg; compared with the group added with 0mg/kg of Chinese herbal medicine extract, the group added with 300mg/kg of Chinese herbal medicine extract obviously improves the digestibility of DM and GE (p is less than 0.05), greatly improves the digestibility of OM, CP and TCHO (p is less than 0.01), and has the tendency of improving the digestibility of EE. Therefore, the Chinese herbal medicine extract disclosed by the patent can be used for diminishing inflammation, reducing the consumption of nutrient substances caused by in-vivo inflammatory reaction, and improving the utilization rate of the nutrient substances, so that the feed conversion rate is improved, and the growth of animals is promoted.
TABLE 9 influence of the addition of herbal extracts on the digestibility of broiler nutrients under different daily ration conditions
And (3) economic benefit analysis: a reduction in feed formulation by 50Mcal results in a 0.028 increase in feed weight ratio and a 1.88 reduction in feed conversion, indicating that 50 kcal energy corresponds to an economic benefit of 3450 x 1.88 ═ 64.86 yuan; the feed conversion rate is improved by 3.14% by reducing the group material ratio of Chinese herbal medicine extracts of 300mg/kg by 0.048, and the corresponding feed value is 3500 x 3.14% to 109.9 yuan. From the production performance, compared with the normal energy group, the weight ratio of 1-42d of the group added with 300mg/kg of the Chinese herbal medicine extract in the energy-reduced daily ration is reduced by 0.22, and the weight of 42d of the chicken is improved by 45g, so that the value of adding 300mg/kg of the Chinese herbal medicine extract in the feed is far greater than the economic value brought by 50 kcal of energy. Under the background of large expansion of feed raw materials and large cost pressure of a feed end formula, the feed additive containing the Chinese herbal medicine extract can be added into the feed, and meanwhile, the formula energy is reduced, so that the aims of reducing cost and improving efficiency are fulfilled.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. A Chinese herbal medicine extract feed additive for bacteriostasis and anti-inflammation is characterized by comprising a lonicera confusa extract, a scutellaria baicalensis extract and a composite fermentation traditional Chinese medicine;
wherein the mass ratio of the lonicera confusa extract to the scutellaria baicalensis extract is 1: 1-6.
2. The Chinese herbal medicine extract feed additive as claimed in claim 1, wherein the content of chlorogenic acid in the lonicera confusa extract is 5-10%;
and/or, the content of the effective component baicalin in the scutellaria baicalensis extract is 50-80%;
preferably, the lonicera confusa extract and the scutellaria baicalensis extract are prepared by adopting an ultrasonic water extraction method.
3. The herbal extract feed additive as claimed in claim 1 or 2, wherein the lonicera confusa extract is prepared in the following way:
mixing flos Lonicerae with water according to a material-liquid ratio of 1:10-1: 15; extracting at 50-80 deg.C for 3-4 hr for 2-4 times to obtain flos Lonicerae extract; then collecting filtrate by ultra-high speed centrifugation, and performing low-temperature vacuum concentration and spray drying to obtain flos Lonicerae extract dry powder with effective component content 5-10% calculated by chlorogenic acid.
4. The herbal extract feed additive of claim 1 or 2, wherein the scutellaria baicalensis extract is prepared in the following manner:
cutting Scutellariae radix into 2-4cm, mixing with water at a ratio of 1:10-1:20, extracting at 50-80 deg.C for 2-3 hr for 3-4 times to obtain Scutellariae radix extractive solution, centrifuging at ultra high speed, collecting filtrate, vacuum concentrating at low temperature, and spray drying to obtain Scutellariae radix extract dry powder with effective component content of 50-80% calculated by baicalin.
5. The herbal extract feed additive according to any one of claims 1 to 4, wherein the mass ratio of the lonicera confusa extract to the scutellaria baicalensis extract is 1:1-4, preferably 1: 2-4.
6. The Chinese herbal medicine extract feed additive according to any one of claims 1 to 5, wherein the complex fermentation Chinese medicine takes two or more of astragalus, liquorice, bran, corn and bean pulp as substrates;
wherein the probiotics in the compound fermentation traditional Chinese medicine is 10-50 hundred million cfu/g;
preferably, the mass ratio of the lonicera confusa extract to the compound fermented traditional Chinese medicine is 1: 2-6;
more preferably, the compound fermentation traditional Chinese medicine is prepared by adopting the following method: inoculating composite fermentation strain, accumulating and fermenting in a fermentation tank for 3-5 days at 25-40 deg.C with water content of 33-42%, and drying with a drum dryer to obtain the final product with beneficial microorganism content of 10-50 hundred million cfu/g.
7. The Chinese herbal medicine extract feed additive as claimed in claim 1, which comprises lonicera confusa extract, scutellaria baicalensis extract and compound fermented traditional Chinese medicine in a mass ratio of 1:2-4: 4-6;
wherein the content of chlorogenic acid as an effective component in the lonicera confusa extract is 5-10%;
the content of effective component baicalin in the Scutellariae radix extract is 50-80%;
the compound fermentation traditional Chinese medicine is obtained by fermenting two or more of astragalus, liquorice, bran, corn and bean pulp serving as substrates; the probiotics accounts for 20-30 hundred million cfu/g;
preferably, the Chinese herbal medicine extract feed additive is prepared by adopting the following method:
(1) extracting the lonicera confusa extract: mixing flos Lonicerae with water at a ratio of 1:10-1:15 by ultrasonic water extraction method, extracting at 50-80 deg.C for 3-4 hr for 2-4 times to obtain flos Lonicerae extract, centrifuging at ultra high speed, collecting filtrate, vacuum concentrating at low temperature, and spray drying to obtain flos Lonicerae extract dry powder with effective component content of 5-10% calculated as chlorogenic acid;
(2) extracting the scutellaria baicalensis extract: taking radix Scutellariae of genuine producing area as raw material, cutting radix Scutellariae into 2-4cm length by ultrasonic water extraction method, mixing with water at a ratio of material to liquid of 1:10-1:20, extracting at 50-80 deg.C for 3-4 times (2-3 hr each time) to obtain radix Scutellariae extractive solution, centrifuging at ultra high speed to collect filtrate, vacuum concentrating at low temperature, and spray drying to obtain radix Scutellariae extract dry powder with effective component content of 50-80% in terms of baicalin;
(3) fermentation of the compound fermented traditional Chinese medicine: taking two or more of astragalus, liquorice, bran, corn and bean pulp as substrates, inoculating a composite fermentation strain, piling and fermenting for 3-5 days in a fermentation tank at the temperature of 25-40 ℃ and the water content of 33-42%, and drying by a roller dryer to prepare the microbial fertilizer, wherein the content of beneficial microorganisms is 20-30 hundred million cfu/g;
(4) and (3) physically mixing the prepared lonicera confusa extract, the scutellaria baicalensis extract and the compound fermented traditional Chinese medicine according to a ratio to obtain the Chinese herbal medicine extract feed additive.
8. The application of the Chinese herbal medicine extract feed additive in broiler feed of any one of claims 1-7, wherein the broiler feed is white feather broiler feed or yellow feather broiler feed.
9. The use of claim 8, wherein the amount of the herbal extract feed additive added to the basal diet is 200-1000 mg/kg; preferably, the addition amount in the basic ration is 200-500 mg/kg.
10. The use of claim 8, wherein the herbal extract feed additive is added to the basal diet in an amount of 250-500 mg/kg.
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CN103933120A (en) * | 2013-01-23 | 2014-07-23 | 青岛康地恩药业股份有限公司 | Honeysuckle/radix scutellariae granules used for control of exogenous fever and upper respiratory infection of livestock and poultry and preparing method thereof |
CN110973349A (en) * | 2019-11-20 | 2020-04-10 | 中基高科(北京)生物技术有限公司 | Anti-diarrhea fermented Chinese herbal medicine feed additive for livestock and poultry and preparation method thereof |
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CN101439090A (en) * | 2007-11-20 | 2009-05-27 | 北京生泰尔生物科技有限公司 | Formulation containing honeysuckle flower and scutellaria, and uses thereof |
CN103933120A (en) * | 2013-01-23 | 2014-07-23 | 青岛康地恩药业股份有限公司 | Honeysuckle/radix scutellariae granules used for control of exogenous fever and upper respiratory infection of livestock and poultry and preparing method thereof |
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