CN105685497A - Fermented feed for improving anti-diarrhea ability of long-haired rabbit and preparation method thereof - Google Patents
Fermented feed for improving anti-diarrhea ability of long-haired rabbit and preparation method thereof Download PDFInfo
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- CN105685497A CN105685497A CN201610043180.0A CN201610043180A CN105685497A CN 105685497 A CN105685497 A CN 105685497A CN 201610043180 A CN201610043180 A CN 201610043180A CN 105685497 A CN105685497 A CN 105685497A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention provides fermented feed for improving the anti-diarrhea ability of a long-haired rabbit and a preparation method thereof. The fermented feed is prepared from the following components: 5 to 8 percent of bean pulp, 3 to 5 percent of barley malt, 10 to 15 percent of apple pomace, 15 to 20 percent of corn germ meal, 25 to 40 percent of thick liquid sprayed corn bran, 8 to 12 percent of rice chaff, 0.1 to 0.3 percent of rhizoma polygonati, 0.25 to 0.5 percent of rhizoma coptidis, 0.1 to 0.3 percent of radix astragali, 0.2 to 0.4 percent of radix glycyrrhizae and 0.2 to 0.5 percent of radix aucklandiae. A process comprises the following steps of smashing all the components, enabling the smashed components to pass through a 40-mesh sieve, adding 0.01 to 0.03 percent of dipotassium hydrogen phosphate, 0.05 to 0.08 percent of ammonium sulfate and 5 to 8 percent of glucose into all the components, uniformly mixing an obtained mixture, then adding 4 to 8 percent of complex probiotic liquid into the obtained mixture, regulating the moisture of total components to be between 40 percent and 50 percent, making a pH (potential of hydrogen) natural, first carrying out forced draft ventilation for aerobic fermentation, and after 12 to 14 hours, loading the total components into a fermentation bag with a one-way respiration valve for fermenting for 48 to 52 hours, so as to obtain the fermented feed. The fermented feed provided by the invention is added into the daily ration of the long-haired rabbit according to a proportion of 15 to 30 percent; the anti-diarrhea ability of the long-haired rabbit can be improved; moreover, the problems of drug residue, drug resistance and the like cannot be caused; further, by adding the fermented feed for a long time, the feed intake can be increased; the immunity and the uniformity of a long-haired rabbit group are obviously improved; the death and culling rate of the long-haired rabbit is decreased.
Description
Technical field
The invention belongs to field of fodder, particularly relate to a kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability and preparation method thereof。
Background technology
In the process of Pili in Fetus of Haired Rabbit large-scale cultivation, diarrhoea is a kind of culture diseases that multiple, mortality rate is high throughout the year。The reason causing the diarrhoea of Pili in Fetus of Haired Rabbit is a lot, and as pathogenic microorganism (clostridieum welchii, escherichia coli, parvovirus, parasite) infects, feeding and management is improper, long-distance transport, seasonal variations, Drug intestinal injury, dyspepsia etc.。Sick rabbit there will be that stomachache flatulence, spirit is depressed, the place that sits around, loose and watery stool are soft, atherosclerotic or watery stools, stench etc., feces has indigested feed granules, cause food conversion rate variance, bring out other diseases, serious meeting causes Pili in Fetus of Haired Rabbit dead, brings huge economic loss to raiser。
At present from veterinary's angle, the Therapeutic Method of Pili in Fetus of Haired Rabbit diarrhoea mostly is interpolation antibiotics, although instant effect, but long-term abuse of antibiotics can cause the drug resistance of harmful bacteria and the organ injury of Pili in Fetus of Haired Rabbit, reduce the immunocompetence of Pili in Fetus of Haired Rabbit, affect the normal growth of Pili in Fetus of Haired Rabbit。Bibliographical information adds probiotic bacteria mycopowder, Chinese medicine etc. to Pili in Fetus of Haired Rabbit can the diarrhoea of auxiliary treatment Pili in Fetus of Haired Rabbit。
Fermented feed pig, cattle, sheep cultivation field research relatively more, Pili in Fetus of Haired Rabbit cultivate area research fewer。This patent from improve Pili in Fetus of Haired Rabbit non-specific immunity, promote intestinal growth, suppress the growth of harmful intestinal tract bacteria, feedstuff the several angle of pretreatment study improve Pili in Fetus of Haired Rabbit diarrhea ability product, improve the diarrhea ability of Pili in Fetus of Haired Rabbit。Inventor herein realizes patent innovation by selecting strain, preferred fermentation substrate composition, three means of optimization for fermentation technology targetedly, such as Clostridium butyricum, bacillus acidophilus, the equal energy metabolism antibacterial substance of bacillus thuringiensis, Pili in Fetus of Haired Rabbit intestinal can be suppressed to common are harmful bacterium；The herb fermentings such as Rhizoma Polygonati, the Radix Astragali, Rhizoma Coptidis, Radix Glycyrrhizae, the Radix Aucklandiae can promote the immunity of Pili in Fetus of Haired Rabbit, stimulates nonspecific immunity, can also promote the discharge of harmful gas in Pili in Fetus of Haired Rabbit intestinal；Fructus Hordei Germinatus can stimulate the growth of Pili in Fetus of Haired Rabbit intestinal rich in beta glucan and soluble fiber。Many technological means of this patent ensure that the practical effect after patent conversion。
The present invention adopts yeast, lactic acid bacteria, Clostridium butyricum, Bacillus licheniformis compounded combination to be fermented bacterium, preferred Chinese medicine and feedstuff compatible combination are as fermentation substrate, utilize the technological process that forced ventilation aerobic fermentation and anaerobic fermentation combine, produce and be specifically designed to the fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability, prove through many group culture experiments, it is possible to significantly improve the diarrhea ability of Pili in Fetus of Haired Rabbit。
Summary of the invention
The technical problem that present invention mainly solves is to provide a kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability and preparation method thereof。Said preparation can improve Pili in Fetus of Haired Rabbit diarrhea ability, promotes the economic benefit of peasant household's cultivation Pili in Fetus of Haired Rabbit。
The present invention realizes above-mentioned technical problem by the following method:
A kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability, described fermentation substrate component includes the raw material of following mass percent:
Bean cake 5%-8%, Fructus Hordei Germinatus 3%-5%, pomace 10-15%, corn germ cake 15-20%, whitewashing skin of Semen Maydis 25%-40%, rice chaff 8%-12% Rhizoma Polygonati 0.1%-0.3%, Rhizoma Coptidis 0.25-0.5%, Radix Astragali 0.1%-0.3%, Radix Glycyrrhizae 0.2%-0.4%, Radix Aucklandiae 0.2%-0.5%, dipotassium hydrogen phosphate 0.01%-0.03%, ammonium sulfate 0.05%-0.08%, glucose 5%-8%。
A kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability, described composite fermentation fluid component includes the bacterium solution of following mass percent:
Concentration is 1*107Saccharomyces cerevisiae (CICC1219) the seed liquor 10%-20% of cfu/ml, concentration is 1*107Clostridium butyricum (CICC10350) the seed liquor 20%-40% of cfu/ml, concentration is 1*107Bacillus acidophilus (CICC6005) the seed liquor 20%-50% of cfu/ml, concentration is 2*107Thuringiensis (CICC21298) the seed liquor 10%-20%. of cfu/ml
A kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability, described preparation method is: first bean cake, Fructus Hordei Germinatus, pomace, corn germ cake, whitewashing skin of Semen Maydis, rice chaff, Rhizoma Polygonati, Rhizoma Coptidis, the Radix Astragali, Radix Glycyrrhizae, the Radix Aucklandiae, dipotassium hydrogen phosphate, ammonium sulfate, glucose etc. are pulverized, cross 40 mesh sieves, fully mix；Secondly: inoculation composite fermentation liquid (saccharomyces cerevisiae (CICC1219) seed liquor, Clostridium butyricum (CICC10350) seed liquor, bacillus acidophilus (CICC6005) seed liquor, thuringiensis (CICC21298) seed liquor), regulating total material moisture between 40%-50%, initial pH is natural；Finally, room temperature controls at 25-30 DEG C, first forced ventilation aerobic fermentation, after 12-14 hour, loads fermentation 48-52 hour in the fermentation bag with one-way breather valve。
Advantages of the present invention and have the benefit effect that quadruple factors such as utilizing specific prebiotic strain, the probiotics of probiotic bacteria metabolism, Chinese medicinal components and feedstuff, from four aspects such as the non-specific immunity of raising Pili in Fetus of Haired Rabbit, the predigestion promoting intestinal growth, the growth of suppression harmful intestinal tract bacteria, help feedstuff, the diarrhea ability of General Promotion Pili in Fetus of Haired Rabbit, reduce Pili in Fetus of Haired Rabbit and the probability of diarrhoea occurs, reduce the use of curative drug, realize green cultivation, increase the culture benefit of Pili in Fetus of Haired Rabbit。
This patent fermentation technology takes the mode that aerobic fermentation and anaerobic fermentation combine, and both ensure that completing of aerobic strain and aerobic biological reaction, has ensured again the abundant growth of anaerobic species, scientific and reasonable。
Accompanying drawing explanation
Fig. 1 is the technological process of a kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability。
Detailed description of the invention
Following example are used for illustrating the present invention, but are not used to limit the scope of the present invention。
Embodiment 1: saccharomyces cerevisiae, bacillus acidophilus, Clostridium butyricum, Su Yun gold bar spore bacillus are inoculated in respective seed liquor culture medium under aseptic condition, 24h is cultivated at 30 DEG C, 37 DEG C, 37 DEG C, 37 DEG C, obtaining the bacterium solution of four kinds of probiotic bacterias, bacterial concentration is followed successively by: 1*107cfu/ml、1*107cfu/ml、1*107cfu/ml、2*107Cfu/ml。According to saccharomyces cerevisiae: Clostridium butyricum: bacillus acidophilus: Su Yun gold bar spore bacillus volume ratio is 10%-20%:20%-40%:20%-50%:10%-20%, makes compound probiotic bacterium solution。
By bean cake, Fructus Hordei Germinatus, pomace, corn germ cake, whitewashing skin of Semen Maydis, rice chaff, Rhizoma Polygonati, Rhizoma Coptidis, the Radix Astragali, Radix Glycyrrhizae, the Radix Aucklandiae, dipotassium hydrogen phosphate, ammonium sulfate, glucose according to mass ratio: 5%-8%:3%-5%:10-15%:15-20%:25%-40%:8%-12%:0.1%-0.3%:0.25-0.5%:0.1%-0.3%:0.2%-0.4%:0.2%-0.5%:0.01%-0.03%:0.05%-0.0 8%:5%-8%, after pulverizing, cross 40 mesh sieves, be sufficiently mixed。
The solid substrate of mixing and compound probiotic liquid being sufficiently mixed, (inoculum concentration of compound probiotic is 8-13%) then regulates material total moisture between 40%-50%, and pH is natural。
Being deposited in fermenting cellar by the fermented product of mix homogeneously, fermenting cellar temperature controls as 25-30 DEG C, at interval of 3 hours, forces to turn ventilation once。After 12-16 hour, charge material in the fermented feed bag with one-way breather valve, then anaerobic fermentation 48-52 hour, sampling observation, saccharomyces cerevisiae viable bacteria concentration >=5*107Cfu/g, clostridium butyricum active bacteria concentration >=2*107Cfu/g, live lactobacillus acidophilus concentration >=3*107Cfu/g, Bacillus thuringiensis viable bacteria concentration >=7*107Cfu/g, is fermentation fully。Escherichia coli, staphylococcus aureus, Salmonella are Gao Min by fermented feed eluent, each strain antibacterial circle diameter >=15mm(Odontothrips loti bacteriostatic test measures antibacterial circle diameter size, antibacterial circle diameter is extremely quick more than 20mm, antibacterial circle diameter 15～20mm is Gao Min, antibacterial circle diameter is 10～15mm be in quick, antibacterial circle diameter is hypoallergenic less than 10mm, and without inhibition zone is insensitive。)。
Embodiment 2: saccharomyces cerevisiae, bacillus acidophilus, Clostridium butyricum, Su Yun gold bar spore bacillus are inoculated in respective seed liquor culture medium under aseptic condition, 24h is cultivated at 30 DEG C, 37 DEG C, 37 DEG C, 37 DEG C, obtaining the bacterium solution of four kinds of probiotic bacterias, bacterial concentration is followed successively by: 1*107cfu/ml、1*107cfu/ml、1*107cfu/ml、2*107Cfu/ml。According to saccharomyces cerevisiae: Clostridium butyricum: bacillus acidophilus: Su Yun gold bar spore bacillus volume ratio is 10%:40%:40%:10%, makes compound probiotic bacterium solution。
By bean cake, Fructus Hordei Germinatus, pomace, corn germ cake, whitewashing skin of Semen Maydis, rice chaff, Rhizoma Polygonati, Rhizoma Coptidis, the Radix Astragali, Radix Glycyrrhizae, the Radix Aucklandiae, dipotassium hydrogen phosphate, ammonium sulfate, glucose according to mass ratio: 5%:3%:15%:20%:40%:8%:0.1%:0.25:0.25%:0.2%:0.5%:0.01%:0.0 5%:7.7%, after pulverizing, cross 40 mesh sieves, be sufficiently mixed。
The solid substrate of mixing and compound probiotic liquid are sufficiently mixed (inoculum concentration of compound probiotic is 8%), then regulate material total moisture pH between 45% natural。
Being deposited in fermenting cellar by the fermentation liquid after mixing, it is 25 DEG C that fermenting cellar temperature controls, and at interval of 3 hours, forces to turn ventilation once。After 14 hours, charge material in the fermented feed bag with one-way breather valve, then anaerobic fermentation 48 hours, inspect probiotic bacteria index, saccharomyces cerevisiae viable bacteria concentration >=5*10 by random samples7Cfu/g, clostridium butyricum active bacteria concentration >=2*107Cfu/g, live lactobacillus acidophilus concentration >=3*107Cfu/g, Bacillus thuringiensis viable bacteria concentration >=7*107Cfu/g, is fermentation fully。Escherichia coli, staphylococcus aureus, Salmonella are Gao Min by fermented feed eluent, each strain antibacterial circle diameter >=15mm(Odontothrips loti bacteriostatic test measures antibacterial circle diameter size, antibacterial circle diameter is extremely quick more than 20mm, antibacterial circle diameter 15～20mm is Gao Min, antibacterial circle diameter is 10～15mm be in quick, antibacterial circle diameter is hypoallergenic less than 10mm, and without inhibition zone is insensitive。)。
Embodiment 3: saccharomyces cerevisiae, bacillus acidophilus, Clostridium butyricum, Su Yun gold bar spore bacillus are inoculated in respective seed liquor culture medium under aseptic condition, 24h is cultivated at 30 DEG C, 37 DEG C, 37 DEG C, 37 DEG C, obtaining the bacterium solution of four kinds of probiotic bacterias, bacterial concentration is followed successively by: 1*107cfu/ml、1*107cfu/ml、1*107cfu/ml、2*107Cfu/ml。According to saccharomyces cerevisiae: Clostridium butyricum: bacillus acidophilus: Su Yun gold bar spore bacillus volume ratio is 20%:20%%:50%:10%, makes compound probiotic bacterium solution。
By bean cake, Fructus Hordei Germinatus, pomace, corn germ cake, whitewashing skin of Semen Maydis, rice chaff, Rhizoma Polygonati, Rhizoma Coptidis, the Radix Astragali, Radix Glycyrrhizae, the Radix Aucklandiae, dipotassium hydrogen phosphate, ammonium sulfate, glucose according to mass ratio: 8%:5%:14%:20%:34%:12%:0.3%:0.5%:0.3%:0.4%:0.5%:0.03%:0.0 8%:5.4%, after pulverizing, cross 40 mesh sieves, be sufficiently mixed。
The solid substrate of mixing and compound probiotic liquid being sufficiently mixed, (inoculum concentration of compound probiotic is 10%) then regulates material total moisture between 47.5%, and pH is natural。
Being deposited in fermenting cellar by the fermentation liquid after mixing, it is 28 DEG C that fermenting cellar temperature controls, and at interval of 3 hours, forces to turn ventilation once。After 16 hours, charge material in the fermented feed bag with one-way breather valve, then anaerobic fermentation 52 hours, sampling observation, saccharomyces cerevisiae viable bacteria concentration >=5*107Cfu/g, clostridium butyricum active bacteria concentration >=2*107Cfu/g, live lactobacillus acidophilus concentration >=3*107Cfu/g, Bacillus thuringiensis viable bacteria concentration >=7*107Cfu/g, it is fermentation fully, escherichia coli, staphylococcus aureus, Salmonella are Gao Min by fermented feed eluent, each strain antibacterial circle diameter >=15mm(Odontothrips loti bacteriostatic test measures antibacterial circle diameter size, antibacterial circle diameter is extremely quick more than 20mm, and antibacterial circle diameter 15～20mm is Gao Min, antibacterial circle diameter is 10～15mm be in quick, antibacterial circle diameter is hypoallergenic less than 10mm, and without inhibition zone is insensitive)。
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the technology of the present invention principle; can also making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention。
Claims (4)
1. the fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability is characterised by, solid constituent is: bean cake 5%-8%, Fructus Hordei Germinatus 3%-5%, pomace 10-15%, corn germ cake 15-20%, whitewashing skin of Semen Maydis 25%-40%, rice chaff 8%-12% Rhizoma Polygonati 0.1%-0.3%, Rhizoma Coptidis 0.25-0.5%, Radix Astragali 0.1%-0.3%, Radix Glycyrrhizae 0.2%-0.4%, Radix Aucklandiae 0.2%-0.5%, dipotassium hydrogen phosphate 0.01%-0.03%, ammonium sulfate 0.05%-0.08%, glucose 5%-8%。
2. the preparation method of a kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability as claimed in claim 1, it is characterized in that probiotic bacteria composite bacteria liquid component is saccharomyces cerevisiae (CICC1219), Clostridium butyricum (CICC10350), bacillus acidophilus (CICC6005), bacillus thuringiensis (CICC21298), content is 10%-20%, 20%-40% respectively, 20%-50%, 10%-20%。
3. the preparation method of a kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability as claimed in claim 1, it is characterised in that regulate total material moisture between 40%-50%, initial pH is natural。
4. the preparation method of a kind of fermented feed improving Pili in Fetus of Haired Rabbit diarrhea ability as claimed in claim 1, fermented product after it is characterized in that mix homogeneously is deposited in fermenting cellar, fermenting cellar temperature controls as 25-30 DEG C, at interval of 3 hours, pressure turns ventilation once, after 12-16 hour, charge material in the fermented feed bag with one-way breather valve, anaerobic fermentation 48-52 hour。
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| CN105994959A (en) * | 2016-06-30 | 2016-10-12 | 河南省岳氏精忠科技有限公司 | Preparation method of lactobacillus acidophilus feed additive |
| CN106138110A (en) * | 2016-01-29 | 2016-11-23 | 江南大学 | A kind of microbial bacterial agent improving obesity and preparation method thereof |
| CN106551134A (en) * | 2016-10-12 | 2017-04-05 | 浙江海洋大学 | A kind of pigletss diarrhea feed additive and preparation method thereof, application |
| CN108576378A (en) * | 2018-05-28 | 2018-09-28 | 天津大成前瞻生物科技研发有限公司 | A kind of sow in lactation fermented feed |
| CN109182177A (en) * | 2018-09-11 | 2019-01-11 | 福建傲农生物科技集团股份有限公司 | A kind of compound probiotic deodorant and the preparation method and application thereof |
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| CN108576378A (en) * | 2018-05-28 | 2018-09-28 | 天津大成前瞻生物科技研发有限公司 | A kind of sow in lactation fermented feed |
| CN109182177A (en) * | 2018-09-11 | 2019-01-11 | 福建傲农生物科技集团股份有限公司 | A kind of compound probiotic deodorant and the preparation method and application thereof |
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Inventor after: Meng Qinglong Inventor after: Zhang Lin Inventor after: Lv Changfeng Inventor after: Xu Yanqun Inventor after: Xie Jikui Inventor after: Xu Bangwei Inventor after: Yu Zheng Inventor after: Li Sai Inventor before: Meng Qinglong Inventor before: Zhang Lin Inventor before: Lv Changfeng |
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Application publication date: 20160622 |
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| WD01 | Invention patent application deemed withdrawn after publication |