CN110859896A - Solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery piglets - Google Patents
Solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery piglets Download PDFInfo
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Abstract
The invention discloses a solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs, which comprises the following components in parts by weight: 35-42 parts of astragalus membranaceus, 30-38 parts of coptis chinensis, 28-35 parts of poplar flower, 22-28 parts of phellodendron, 22-25 parts of sophora flavescens, 22-28 parts of bighead atractylodes rhizome, 18-20 parts of dark plum, 18-25 parts of pomegranate bark, 16-22 parts of dandelion, 12-16 parts of rhizoma alismatis, 10-15 parts of hairyvein agrimony, 10-15 parts of purslane, 10-15 parts of liquorice, and a plurality of bacillus licheniformis and lactobacillus acidophilus for fermentation and fermentation products thereof. The traditional Chinese medicine fermentation preparation provided by the invention has the advantages that the traditional Chinese medicine is used as a fermentation raw material, the optimized bacillus licheniformis and lactobacillus acidophilus are utilized for biotransformation, the traditional Chinese medicine microecological preparation which is non-toxic, free of residue, green, easy to absorb and obviously enhanced in antibacterial activity is prepared, the drug effect is obviously improved, the preparation method of the traditional Chinese medicine fermentation preparation is simple and reasonable, the traditional Chinese medicine and probiotics are combined in the traditional Chinese medicine fermentation preparation, the production period is short, and the cost is low.
Description
Technical Field
The invention relates to the technical field of fermented traditional Chinese medicine additives, in particular to a solid-state fermented traditional Chinese medicine additive for preventing and nursing piglet diarrhea by replacing antibiotics.
Background
In modern pig raising production, the phenomenon of diarrhea of weaned piglets is serious, and the reason is also complicated. However, the diarrhea of the weaned piglets is not easy to be paid attention to, some diseases seriously form the cad pigs and even die, and direct and huge economic influence is brought to the pig raising production. The reason for causing diarrhea in weaned piglets is as follows:
the self factors are as follows: the digestion ability is not good. The gastrointestinal digestive system of the newborn piglet is not completely developed, and the newborn piglet can grow by depending on rich nutrition in breast milk in the lactation period. But in a period of time after weaning, the gastric acid secretion in the bodies of the piglets is insufficient, and the content of digestive enzymes is in a lower level, so that some undigested protein feeds are fermented in small intestines, the normal flora proportion of the small intestines is disturbed, pathogenic escherichia coli rapidly grow, and then small intestinal mucosa is badly stimulated, and diarrhea symptoms appear. The immune system is immature. Most mammals rely on breast milk for active immunity for a period of time after birth. The active immune acquisition pathway is cut off after weaning, and the weaning is also a stress, which has a plurality of adverse effects on the development of the immune system of the piglets. The antibody level in the piglet body is low, the resistance to the change of the external environment is weak, a plurality of hotbed environments in a pigsty are not ideal, and a plurality of pathogenic bacteria can enter in a conquering way to cause diarrhea of the weaned piglet.
Environmental factors: the temperature difference in the house is too large. The fat layer of the piglet is thin and is not enough to resist large external temperature difference change. When the temperature change in the piggery exceeds 10 ℃, the digestive system of the piglets is obstructed. Research shows that in a certain temperature difference range, the possibility of diarrhea of piglets is increased by about 30% when the temperature changes by more than 10 ℃.
The feed proportioning is not scientific: although a certain amount of fiber can be digested by a pig serving as a omnivorous animal, the digestive organs of piglets lack enough enzymes capable of digesting the cellulose, so that the fiber feed cannot be fully digested in intestinal tracts of the piglets, gastrointestinal mucosa of the piglets is damaged, normal flora of the intestinal tracts is damaged, and diarrhea is caused.
Threat of pathogenic bacteria. Digestive tract diseases are mostly caused by contamination with pathogenic microorganisms. In a pig farm, the potential threat of various pathogenic bacteria can be generated once a scientific disinfection process is lacked. The most common of these are E.coli and Salmonella. This is often the case in current pig farms where the diarrhea is common and once the piglets become infected with such pathogenic microorganisms, they are difficult to treat.
The conventional method for preventing and nursing piglets comprises the following steps: florfenicol, neomycin, quinolone and other medicines are generally selected, and the florfenicol, neomycin, quinolone and other medicines are matched to obtain better effects such as Shuanghuanglian, atropine, glucose, camphor, vitamin and the like. Secondly, in the treatment process, comprehensive measures such as laxation, antibiosis and antiphlogosis, fluid infusion, cardiotonic, nutrition, heat preservation, pressure prevention and the like must be adhered to.
The method reduces the diarrhea of the weaned piglets to a certain extent, but the drug resistance of the piglets is increased by using the antibiotics for a long time, and the effect is worse and worse.
Therefore, the application provides a solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs and a preparation method thereof.
Disclosure of Invention
The invention aims to provide a solid-state fermentation traditional Chinese medicine additive for preventing diarrhea of nursery pigs by replacing antibiotics and a preparation method thereof, and aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent nursery pig diarrhea comprises the following components in parts by weight: 35-42 parts of astragalus membranaceus, 30-38 parts of coptis chinensis, 28-35 parts of poplar flower, 22-28 parts of phellodendron, 22-25 parts of sophora flavescens, 22-28 parts of bighead atractylodes rhizome, 18-20 parts of dark plum, 18-25 parts of pomegranate bark, 16-22 parts of dandelion, 12-16 parts of rhizoma alismatis, 10-15 parts of hairyvein agrimony, 10-15 parts of purslane, 10-15 parts of liquorice, and a plurality of bacillus licheniformis and lactobacillus acidophilus for fermentation and fermentation products thereof.
As a further scheme of the invention: the raw materials comprise the following components in parts by weight: 40 parts of astragalus membranaceus, 30 parts of coptis chinensis, 28 parts of poplar flower, 22 parts of phellodendron, 22 parts of sophora flavescens, 22 parts of bighead atractylodes rhizome, 18 parts of dark plum, 18 parts of pomegranate rind, 16 parts of dandelion, 12 parts of rhizoma alismatis, 10 parts of hairyvein agrimony, 10 parts of purslane and 10 parts of liquorice.
A preparation method of a solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs comprises the following specific steps:
s1: selecting traditional Chinese medicine extracts, weighing corresponding parts of astragalus membranaceus, coptis chinensis, poplar flowers, phellodendron, radix sophorae flavescentis, bighead atractylodes rhizome, dark plum fruits, pomegranate rind, dandelion, rhizoma alismatis, hairyvein agrimony, purslane and liquorice, and crushing to form mixed powder;
s2, uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, and fermenting at 36-38 ℃ for 24-48 h to obtain fermented powder;
and S3, drying and subpackaging the solid fermentation mixture and warehousing.
As a further scheme of the invention: the formula of the fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
As a further scheme of the invention: the probiotics are bacillus licheniformis and lactobacillus acidophilus.
As a further scheme of the invention: the fermentation is carried out in a fermentation tank, and the conditions of the fermentation are as follows: the pressure is 0.02 kPa-0.1 kPa, the stirring speed is 10 r/min-30 r/min, and the pH value is 6.0-7.0.
As a further scheme of the invention: the adding ratio of the mixed powder to the fermentation medium is (8-20) g: 1L.
As a further scheme of the invention: the inoculated probiotics are second-stage seed liquid of probiotic strains, and the preparation steps of the second-stage seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12-24 h at 36-38 ℃ to obtain a primary seed solution;
(2) inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and performing static culture at 36-38 ℃ for 12-24 h to obtain a secondary seed solution;
(3) and inoculating the secondary seed liquid according to the volume ratio of the secondary seed liquid to the fermentation culture medium of 3-8 vol%.
As a further scheme of the invention: the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5 g of yeast extract, 20 g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
The mechanism for preventing and controlling the piglet diarrhea is that the traditional Chinese medicine extract enables glycosides, saponins, aglycones, flavones, terpenes, sterols and the like in the effective components of the traditional Chinese medicine to have biological activity, and promotes and amplifies the antibacterial and immune functions of the traditional Chinese medicine extract by a fermentation technology. The using method comprises the following steps: nursing piglets and piglets, mixing 1000g/T of feed, and feeding for 5-7 days.
The coptis root is also named as Chuan Lian, Chuan Lian Huang Lian, ginger processed coptis root, ginger Lian, Yu Lian, etc. Is the dried rhizome of goldthread root, or Yunnan coptis root and trigonella belonging to Ranunculaceae, belongs to heat-clearing drugs, and is listed as the first-class product in Shen nong Ben Cao Jing. The rhizoma coptidis mainly comprises berberine and coptisine with a molecular formula of C19H15NO5, the relative molecular mass is 337.32, palmatine (named as Bamadin) and a molecular formula of C21H22NO4, and the relative molecular mass is 352.40, magnoline, 2,3, 4-trihydroxy phenylpropyl and 3-carboxyl-4-hydroxyphenoxy glucose. The main functions of coptis are: the berberine hydrochloride has the functions of resisting inflammation and relieving fever, and has the function of inhibiting acute and chronic inflammations. The anti-inflammatory action of berberine is related to some links of the inflammatory process, such as coptis root and berberine which can enhance the phagocytic function of white blood cells in vivo and in vitro. Coptis chinensis Franch can produce antipyretic effect by inhibiting the generation or release of central fever medium, such as antipyretic Coptis chinensis Franch injection has obvious antipyretic effect on experimental fever, and can reduce cAMP content in cerebrospinal fluid; secondly, the coptis chinensis and the berberine hydrochloride can resist the toxins of various pathogenic microorganisms, improve the tolerance of organisms to bacterial endotoxin and resist the diarrhea caused by escherichia coli. In addition, berberine can also resist diarrhea and noninfectious diarrhea caused by cholera toxin, relieve inflammatory reactions such as edema and hypersecretion of intestinal villi, and reduce death rate; thirdly, the Chinese goldthread and berberine have broad-spectrum antibacterial action and can inhibit or kill various bacteria, tubercle bacillus, fungi and the like. The literature reports that the coptis and the berberine hydrochloride have the inhibiting effect on 7 16 pathogenic bacteria including hemolytic streptococcus, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, dysentery bacillus, cloaca bacillus and typhoid bacillus. In addition, Coptidis rhizoma and SANHUANG injection (composed of equal amount of Coptidis rhizoma, Scutellariae radix, and cortex Phellodendri) also have antifungal effect, Coptidis rhizoma decoction and berberine hydrochloride have in vitro and in vivo anti-Ammi effect, and berberine hydrochloride has inhibitory effect on various influenza viruses. It is worth noting that the coptis root is bacteriostatic at low concentration and bactericidal at high concentration, and the antibacterial action links are generally as follows: the method is characterized by comprising the steps of destroying the structure of bacteria, inhibiting bacterial sugar metabolism (coptis can inhibit the oxidative decarboxylation process of yeast and intermediate link pyruvic acid of bacterial sugar metabolism), and inhibiting nucleic acid and protein synthesis.
Cortex Phellodendri is dried bark of phellodendron amurense or phellodendron amurense belonging to Rutaceae. The former is commonly called Chuan Huang Bai, the latter is commonly called Guan Huang Bai. The phellodendron amurense is a plant or arbor of the genus phellodendron of the family Rutaceae and is mainly distributed in Shaanxi and Sichuan provinces of China.
The main chemical components of the golden cypress are flavonoids and alkaloids, wherein the alkaloids are the main effective components of the golden cypress and have the highest content. In addition, the alkaloids include berberine, jateorhizine, magnoflorine, phellodendrine, tetrandrine, lactone, sterol, mucoid, etc. The cortex Phellodendri contains alkaloid and flavone, obacunone, lactone, lactide, obacunone acid, fluocinolone, 7-dehydrostigmasterol, p-sitosterol, campesterol, etc. At present, the HPLC method is the main method for measuring the content of the active ingredients of the phellodendron amurense and the phellodendron amurense.
MoirH et al report that phellodendron bark has the effect of suppressing cellular immune response, phellodendrine and magnoflorine are main active substances thereof, and they can suppress host reaction (GvH) of local transplanted tissue of mice, and also can suppress induction phase of picryl chloride induced Delayed Type Hypersensitivity (DTH) of mice, but do not suppress reaction phase. Lvyanning et al observed the effect of phellodendron on several important cytokines and their DTH in mice with Dinitrofluorobenzene (DNFB) mouse model, found that phellodendron can inhibit DNFB-induced DTH in mice to inhibit immune response and reduce inflammatory injury.
Radix Sophorae Flavescentis is dried root of radix Sophorae Flavescentis of Leguminosae. In northern Hebei, West and Henan, southeast and southwest of Shandong, Anhui, Hubei, Guizhou, etc. areas of China. It is listed as a middle-grade product in Shen nong Ben Cao Jing (Shen nong's herbal Jing), extremely bitter in taste and cold in nature, and has the functions of clearing heat, drying dampness, killing parasites, inducing diuresis and the like. Kuh-seng belongs to heat-clearing and dampness-drying herbs in heat-clearing herbs and is used for heat dysentery, hematochezia, eczema and skin pruritus. The main pharmacological actions of the sophora flavescens include anti-inflammation, anti-pathogenic microorganism, anti-liver injury, and regulation of immune system and nervous system.
The chemical components of the sophora flavescens are as follows: the matrine is alkaloid, the matrine is mostly quinolizidine, and the matrine is rarely bispidine; secondly, flavonoids are obtained, so far, 108 flavonoids are obtained by co-separating flavonoids in sophora flavescens, wherein isopentenyl side chains exist on the A ring of most compounds; and the third is fatty acids and volatile oils, and the fatty acids comprise methyl acetate, methyl dodecanoate, cetyl erucate and the like. The volatile oil contains 47 components such as hexanal, n-nonanoic acid, 1-octen-5-ol, etc., and provides a substance reference for the special fragrance of radix Sophorae Flavescentis; fourthly, the lightyellow sophora root also contains chemical components such as amino acids, saccharides, triterpenes, triterpenoid saponins, lignans, phenolic acids and the like.
For review of the action of kuh-seng against pathogenic microorganisms: yi Changjiang river and the like use 10 kinds of common Chinese herbal medicines such as honeysuckle, coptis chinensis, scutellaria baicalensis, sophora flavescens and the like to extract sterile liquid medicine, the size of an inhibition zone of the Chinese herbal medicine extracting solution to the separation of methicillin-resistant staphylococcus aureus (MRSA) from clinical specimens is determined by adopting a perforation method, and the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the Chinese herbal medicine extracting solution to the MRSA are determined by a 2-fold dilution method. The result shows that the sophora flavescens has a certain antibacterial effect on MRSA clinical strains. Qinqinging and the like adopt a standard agar plate double dilution method to measure the Minimum Inhibitory Concentration (MIC) of emodin, berberine, matrine, baicalin and the like to sensitive and drug-resistant escherichia coli, and observe the inhibition effect of the traditional Chinese medicine extract to the sensitive and drug-resistant escherichia coli. The results showed that the MIC of matrine to sensitive E.coli was 64. mu.g/mL and the MIC to drug resistant E.coli was 128. mu.g/mL. The matrine has certain inhibition effect on sensitive and drug-resistant escherichia coli. The Huang-Yan Qiang adopts a standard agar plate double dilution method to measure the Minimum Inhibitory Concentration (MIC) of emodin, berberine, matrine, baicalin and the like to the drug-resistant helicobacter pylori, detects the absorbance (A) value of a reaction hole after the strain acts on the MIC drug, observes the formation condition of the drug-resistant helicobacter pylori biofilm through a confocal microscope, and observes the influence of the traditional Chinese medicine extract on the formation of the drug-resistant helicobacter pylori biofilm. The results show that the MIC of matrine to drug-resistant helicobacter pylori is 128 mug/mL, and the biofilm formation under a confocal microscope is obviously less when the matrine is 64 mug/mL. The matrine is suggested to have an inhibiting effect on drug-resistant helicobacter pylori, and the concentration of MIC is less than 50 percent, so that the matrine can obviously inhibit the formation of bacterial strain biofilms. The mansion tourmaline and the like show that the kusheng water decoction has obvious inhibition effect on escherichia coli, staphylococcus aureus, streptococcus A, streptococcus B, dysentery bacillus, salmonella weizili and proteus.
Stimulation of intestinal mucosal immunity by probiotics: the microecological preparation is taken up by the specific M cells in the Peyer's disease knot on the mucosa after entering the digestive tract, is transferred to the antigen presenting cells in PP, induces T cells to generate IL-4, IL-10 and transfer factor by low-dose antigen, inhibits the differentiation of Th1 cells, enables the organism to generate oral tolerance, enables Th0 cells to differentiate to Th1 cells, and stimulates B cells to secrete IgE. High doses of antigen can lead to clonal anergy, T cells are in a cell unresponsive state, T cells are unable to secrete IL-2 and proliferate. The most suitable dosage of antigen can promote the differentiation of Th0 cells to Th1 cells, Th1 cells can promote B cells to secrete SIgA and inhibit IgE secretion, and some cytokines produced by the antigen can inhibit the growth of virus.
The bacillus licheniformis and lactobacillus acidophilus have excellent production performance, have the characteristics of heat resistance, easy storage and adaptation to the micro-ecological environment (anaerobism, acid and bile salt) of animal intestinal tracts, have important significance for reducing the abuse of feed antibiotics in the current animal production, reducing the residue of medicaments in livestock and poultry products such as meat, eggs, milk and the like, reducing the drug resistance of animal bacteria and ensuring the health of animals, are feed probiotics with great potential, and have wide market prospect.
After the compound formula traditional Chinese medicine extract is fermented by a compound microecological preparation (bacillus licheniformis and lactobacillus acidophilus), the macromolecular effective components are changed into micromolecular effective components which are easier to digest and absorb, and the medicine property is improved. Meanwhile, the compound microecological preparation is the most loyal and sanitary for maintaining intestinal health, and the problem of diarrhea of piglets which is complicated can be quickly solved.
Compared with the prior art, the invention has the beneficial effects that:
the traditional Chinese medicine fermentation preparation provided by the invention has the advantages that the traditional Chinese medicine is used as a fermentation raw material, the intermediate substance is subjected to biotransformation by utilizing the optimized bacillus licheniformis and lactobacillus acidophilus to prepare the traditional Chinese medicine microecological preparation which is non-toxic, green, easy to absorb and obviously enhanced in antibacterial activity, so that the drug effect is obviously improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the invention, the solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent the diarrhea of the nursery pigs has the following basic composition data of a traditional Chinese medicine compound formula as shown in a table 1:
table 1, solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery piglets
The Chinese medicinal extracts are weighed according to the requirements of table 1, and the fermented Chinese medicinal additive is prepared according to the following steps.
S2, uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, and fermenting at 36-38 ℃ for 24-48 h to obtain a solid traditional Chinese medicine fermentation preparation;
s3, respectively subpackaging the solid Chinese medicine fermentation preparations and marking for later use.
Examples
Shandong Shen county Dong Tian village Wang (Shandong Shen county). 1390635
300 sows are stored in 18 columns in 5 months in 2019, and 240 weaned piglets of 25 days old are transferred to a nursery house.
240 weaned piglets are divided into 3 groups, a solid-state fermentation traditional Chinese medicine additive (which is doubled for the first time according to 1000 g/ton of feed for continuous use for 5 days) for replacing antibiotics to prevent piglet diarrhea is added into drinking water of the test 1 group, a solid-state fermentation traditional Chinese medicine additive (which is doubled for the first time according to 1000 g/ton of feed for continuous use for 5 days) for replacing antibiotics to prevent piglet diarrhea is added into drinking water of the test 2 group, and the 3 rd group is a control group (which is added with antibiotics regularly according to conventional health care) shown in table 2.
TABLE 2 solid fermentation Chinese medicine additive application test starting basic data for replacing antibiotics to prevent nursery pig diarrhea
At the beginning of the test, the number of each group was consistent, and the body weight was consistent (the difference was not significant).
After the 60-day-old test, the production data were obtained as follows:
at the end of the test, the body weight of the test group exceeded that of the control group (the difference was significant); the activity rate of the test composition (97.5-98.8%) is higher than that of the control group (the difference is obvious).
At the end of the test, the incidence rate of piglet diarrhea in the test group is 0, which is lower than that in the control group, and the difference is obvious.
In summary, the following steps: the traditional Chinese medicine microecological preparation provided by the invention is reasonable in component proportion, takes traditional Chinese medicines as fermentation raw materials, utilizes the optimized clostridium butyricum and lactic acid bacteria to carry out biotransformation on intermediate substances, and is prepared into a non-toxic, residue-free, green, easily-absorbed and obviously-enhanced traditional Chinese medicine microecological preparation, so that the drug effect is obviously improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (9)
1. A solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent nursery pig diarrhea is characterized in that the raw materials comprise the following components in parts by weight: 35-42 parts of astragalus membranaceus, 30-38 parts of coptis chinensis, 28-35 parts of poplar flower, 22-28 parts of phellodendron, 22-25 parts of sophora flavescens, 22-28 parts of bighead atractylodes rhizome, 18-20 parts of dark plum, 18-25 parts of pomegranate bark, 16-22 parts of dandelion, 12-16 parts of rhizoma alismatis, 10-15 parts of hairyvein agrimony, 10-15 parts of purslane, 10-15 parts of liquorice, and a plurality of bacillus licheniformis and lactobacillus acidophilus for fermentation and fermentation products thereof.
2. The solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs according to claim 1, is characterized in that raw materials comprise the following components in parts by weight: 40 parts of astragalus membranaceus, 30 parts of coptis chinensis, 28 parts of poplar flower, 22 parts of phellodendron, 22 parts of sophora flavescens, 22 parts of bighead atractylodes rhizome, 18 parts of dark plum, 18 parts of pomegranate rind, 16 parts of dandelion, 12 parts of rhizoma alismatis, 10 parts of hairyvein agrimony, 10 parts of purslane and 10 parts of liquorice.
3. The preparation method of the solid-state fermentation traditional Chinese medicine additive for preventing the diarrhea of the nursery pig instead of the antibiotic according to any one of claims 1 to 2 is characterized by comprising the following specific steps:
s1: selecting traditional Chinese medicine extracts, weighing corresponding parts of astragalus membranaceus, coptis chinensis, poplar flowers, phellodendron, radix sophorae flavescentis, bighead atractylodes rhizome, dark plum fruits, pomegranate rind, dandelion, rhizoma alismatis, hairyvein agrimony, purslane and liquorice, and crushing to form mixed powder;
s2, uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, and fermenting at 36-38 ℃ for 24-48 h to obtain a liquid traditional Chinese medicine fermentation mixture;
s3, drying the liquid traditional Chinese medicine fermentation mixture at 30-50 ℃, and subpackaging and warehousing.
4. The preparation method of the solid-state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs according to claim 3, wherein the formula of the fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
5. The method for preparing the solid state fermentation traditional Chinese medicine additive for preventing diarrhea of nursery pigs according to claim 3, wherein the probiotics are Bacillus licheniformis and Lactobacillus acidophilus.
6. The preparation method of the solid-state fermentation traditional Chinese medicine additive for preventing the diarrhea of the nursing piglets, which is used for replacing antibiotics, is characterized in that the fermentation is carried out in a fermentation tank, and the fermentation conditions are as follows: the pressure is 0.02 kPa-0.1 kPa, the stirring speed is 10 r/min-30 r/min, and the pH value is 6.0-7.0.
7. The preparation method of the solid state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs according to claim 3, wherein the adding ratio of the mixed powder to the fermentation medium is (8-20) g: 1L.
8. The method for preparing the solid state fermentation traditional Chinese medicine additive for preventing the diarrhea of the nursery pig instead of the antibiotic according to the claim 3, characterized in that the inoculated probiotics is a secondary seed liquid of probiotic strains, and the preparation steps of the secondary seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12-24 h at 36-38 ℃ to obtain a primary seed solution;
(2) inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and performing static culture at 36-38 ℃ for 12-24 h to obtain a secondary seed solution;
(3) and inoculating the secondary seed liquid according to the volume ratio of the secondary seed liquid to the fermentation culture medium of 3-8 vol%.
9. The preparation method of the solid state fermentation traditional Chinese medicine additive for replacing antibiotics to prevent diarrhea of nursery pigs according to claim 8, wherein the formula of the seed culture medium for preparing the primary seed liquid and the secondary seed liquid is as follows: 10 g of refined peptone, 5 g of yeast extract, 20 g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
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CN115812866A (en) * | 2021-11-10 | 2023-03-21 | 四川恒博生物科技有限公司 | A Chinese medicinal composition feed additive and its preparation method |
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