CN109718269A - A kind of Chinese medicine microecological preparation and preparation method thereof - Google Patents
A kind of Chinese medicine microecological preparation and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine microecological preparations, including fermenting microbe, Chinese medicine compound prescription, nutriment composition and pure water, the fermenting microbe is lactic acid bacteria, the Chinese medicine compound prescription is as follows according to quality component meter: 5~15 parts of Radix Astragali, 10~30 parts of dandelion, 10~30 parts of folium isatidis, the nutriment composition include glucose, yeast powder, peptone；The present invention also provides a kind of preparation methods of Chinese medicine microecological preparation.A kind of Chinese medicine microecological preparation composition proportion provided by the invention is reasonable, using Chinese medicine as fermentation raw material, bioconversion is carried out to intermediate material using preferred lactic acid bacteria, nontoxic, green, easy absorption is made, the Chinese medicine microecological preparation that antibacterial activity is remarkably reinforced, make drug effect be improved significantly, a kind of preparation method advantages of simple of Chinese medicine microecological preparation provided by the invention, ferments to Chinese medicine, so that the advantages of fermented tcm combines traditional Chinese medicine and probiotics.
Description
Technical field
The present invention relates to livestock culture technical field, specially a kind of Chinese medicine microecological preparation, meanwhile, the invention further relates to
A kind of preparation method of Chinese medicine microecological preparation.
Background technique
Currently, approved nearly tens kinds of antibiotic in China's are as feed addictive.Application of the antibiotic as feed addition
Huge contribution has been made for the fast development of livestock and poultry animal husbandry, using effect and be that other are any to the facilitation of animal husbandry
What a kind of feed addictive can not all be compared with any one technology.But but deepen continuously with scientific and technological and understanding, people
Start to find drawback caused while antibiotic brings great economic benefit as feed addictive.These objects are used for a long time
Matter not only easily causes the problems such as pathogenic microorganism generates drug resistance, animal immunizing power declines and deterioration in quality, and can cause
Hazard residue weight gain in livestock products and environment, seriously threatens human health.Therefore, effective for seeking prevention and treatment Animal diseases
Rate height, noresidue, safe and reliable new medicinal preparation are most important.
The immunity of animal body is that microorganism and harmful substance are resisted and removed to body, reduction stress, to keep and extensive
The ability of multiple normal physiological function.Chinese herbal medicine has multiple nutritional components and bioactivity, has the dual work of nutrition and drug
With, not only disease preventing and treating but also production performance can be improved, it not only can directly antibacterial and sterilization, moreover it is possible to the immune function of body is adjusted,
With nospecific immunity bacteriostasis.
Chinese medicine preparation technique relatively falls behind at present, the basic method for using boiling, since boiling was boiled by time, temperature
The limitation of the factors such as degree, pressure, effective component therein can not be extracted completely, therefore cause wave to a certain extent
Take.
Therefore, it is proposed that a kind of Chinese medicine microecological preparation and preparation method thereof.
Summary of the invention
The purpose of the present invention is to provide a kind of Chinese medicine microecological preparations and preparation method thereof, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the invention provides the following technical scheme:
A kind of Chinese medicine microecological preparation, including fermenting microbe, Chinese medicine compound prescription, nutriment composition and pure water；
The fermenting microbe is lactic acid bacteria, and the inoculum concentration of the lactic acid bacteria is the 3% of inoculum volume；
The Chinese medicine compound prescription is as follows according to quality component meter: 5~15 parts of Radix Astragali, 10~30 parts of dandelion, folium isatidis 10~
30 parts, Radix Astragali has effects that tonifying Qi and lifting yang, invigorating qi for consolidating superficies, and folium isatidis and dandelion have clearing heat and detoxicating, the function of normalizing gallbladder to cure jaundice
Effect；
The nutriment composition includes glucose, yeast powder, peptone.
Preferably, the lactic acid bacteria is Lactobacillus salivarius, the viable count of the Lactobacillus salivarius be greater than or equal to (1.2~
2.5)×109Cfu/mL, the Lactobacillus salivarius are the bacterial strain that deposit number is CGMCC No.14458.
The present invention also provides a kind of preparation methods of Chinese medicine microecological preparation, comprising the following steps:
S1, Radix Astragali, dandelion and folium isatidis are weighed according to suitable quality proportioning；
S2, Radix Astragali weighed in S1 is cut into blocky, dandelion and after folium isatidis is cut into strip, then by block-like Radix Astragali
And strip dandelion and folium isatidis be uniformly mixed, obtain Chinese medicine compound prescription；
S3, Chinese medicine compound prescription obtained in S2 is put into Chinese medicine decocting device, pure water is added, water is decocted 3 times, filtered out respectively
3 filtrates are merged and are concentrated into the medical fluid that raw drug content is 0.5g/mL by filtrate；
S4,5g glucose is added with every hydrargyrum oxydatum crudum liquid, 3g yeast powder and 3g peptone weigh suitable glucose, yeast powder and
Weighed glucose, yeast powder and peptone are obtained nutriment composition by peptone after mixing；
S5, nutriment composition obtained in S4 is added in medical fluid obtained in S3, is uniformly mixed, then benefit
PH value is adjusted to 7.0 with PH regulator, obtains culture solution after sterilized, natural cooling；
S6, lactic acid bacteria is accessed in culture solution with the inoculum concentration of nutrient solution volume 3% obtained in S4, under the conditions of 37 DEG C
Ferment 60~84h after to get arrive Chinese medicine microecological preparation；
S7, preservation will be put in storage after the packing of Chinese medicine microecological preparation obtained in S5.
Preferably, the length of block-like Radix Astragali and wide no more than 1 ㎝, the dandelion and folium isatidis of the strip in the S2
Length no more than 2 ㎝.
Preferably, Chinese medicine decocting device is the double boiler thermally conductive by heat medium in the S3, it is ensured that heat when decoction
The uniformity of conduction avoids hot-spot and influences to decoct effect.
Preferably, water decoction in the S3 method particularly includes:
The pure water that 4 times of volumes are added in Chinese medicine compound prescription carries out first time decoction, and decocting time is 40~60min, mistake
Filter；
The pure water that 3 times of volumes are added in filter residue carries out second and decocts, and decocting time is 40~60min, filtering；
The pure water that 3 times of volumes are added in filter residue carries out third time decoction, and decocting time is 40~60min, filtering.
Preferably, the Chinese medicine compound prescription in the S3 impregnates 20~30min before decoction, and soaking temperature is 25~35 DEG C.
Preferably, the speed of agitator being stirred in the S4 is 200~400r/min, and whipping temp is 25~45 DEG C.
Preferably, the autoclaving that sterilizing methods are 121 DEG C in the S5, sterilization time are 20~30min.
Preferably, the storage temperature of the Chinese medicine microecological preparation after dispensing in the S7 is 4 DEG C.
Compared with prior art, the beneficial effects of the present invention are: a kind of Chinese medicine microecological preparation ingredient provided by the invention
Proportion rationally, under instruction of Chinese Medicine theory, using Chinese medicine as fermentation raw material, gives birth to intermediate material using preferred lactic acid bacteria
Object conversion is made nontoxic, green, easy absorption, the Chinese medicine microecological preparation that antibacterial activity is remarkably reinforced, changes drug effect obviously
Kind, a kind of preparation method advantages of simple of Chinese medicine microecological preparation provided by the invention ferments to Chinese medicine, so that in fermentation
Medicine combines the advantages of traditional Chinese medicine and probiotics, and with short production cycle, and cost is relatively low.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, not
For limiting the present invention.
A kind of Chinese medicine microecological preparation provided by the invention, the base-material composition and specific embodiment data of Chinese medicine compound prescription are such as
Following table:
| Base-material composition | Embodiment 1 (part) | Embodiment 2 (part) | Embodiment 3 (part) |
| Radix Astragali | 5 | 10 | 15 |
| Dandelion | 10 | 20 | 30 |
| Folium isatidis | 10 | 20 | 30 |
Embodiment 1
A kind of Chinese medicine microecological preparation, including fermenting microbe, Chinese medicine compound prescription, nutriment composition and pure water, it is described
Fermenting microbe is lactic acid bacteria, and the inoculum concentration of the lactic acid bacteria is the 3% of inoculum volume, and the Chinese medicine compound prescription is according to quality component
Count as follows: 5 parts of Radix Astragali, 10 parts of dandelion, 10 parts of folium isatidis, the nutriment composition include glucose, yeast powder, albumen
Peptone.
The lactic acid bacteria is Lactobacillus salivarius, and the viable count of the Lactobacillus salivarius is greater than or equal to 1.2 × 109cfu/
ML, the Lactobacillus salivarius are the bacterial strain that deposit number is CGMCC No.14458.
The present invention also provides a kind of preparation methods of Chinese medicine microecological preparation, comprising the following steps:
S1, Radix Astragali, dandelion and folium isatidis are weighed according to suitable quality proportioning；
S2, Radix Astragali weighed in S1 is cut into blocky, dandelion and after folium isatidis is cut into strip, then by block-like Radix Astragali
And strip dandelion and folium isatidis be uniformly mixed, obtain Chinese medicine compound prescription；
S3, Chinese medicine compound prescription obtained in S2 is put into Chinese medicine decocting device, pure water is added, water is decocted 3 times, filtered out respectively
3 filtrates are merged and are concentrated into the medical fluid that raw drug content is 0.5g/mL by filtrate；
S4,5g glucose is added with every hydrargyrum oxydatum crudum liquid, 3g yeast powder and 3g peptone weigh suitable glucose, yeast powder and
Weighed glucose, yeast powder and peptone are obtained nutriment composition by peptone after mixing；
S5, nutriment composition obtained in S4 is added in medical fluid obtained in S3, is uniformly mixed, then benefit
PH value is adjusted to 7.0 with PH regulator, obtains culture solution after sterilized, natural cooling；
S6, lactic acid bacteria is accessed in culture solution with the inoculum concentration of nutrient solution volume 3% obtained in S4, under the conditions of 37 DEG C
Ferment 60h after to get arrive Chinese medicine microecological preparation；
S7, preservation will be put in storage after the packing of Chinese medicine microecological preparation obtained in S5.
The length of block-like Radix Astragali and wide no more than 1 ㎝, the dandelion of the strip and the length of folium isatidis in the S2
No more than 2 ㎝, Chinese medicine decocting device is the double boiler thermally conductive by heat medium in the S3, and water decocts specific in the S3
Method are as follows: the pure water that 4 times of volumes are added in Chinese medicine compound prescription carries out first time decoction, and decocting time 40min is filtered, filter
The pure water that 3 times of volumes are added in slag carries out second and decocts, decocting time 40min, filtering, adds 3 times of bodies in filter residue
Long-pending pure water carries out third time decoction, and decocting time 40min is filtered, and the Chinese medicine compound prescription in the S3 is impregnated before decoction
20min, soaking temperature are 25 DEG C, and the speed of agitator being stirred in the S4 is 200r/min, and whipping temp is 25 DEG C, described
Sterilizing methods are 121 DEG C of autoclaving in S5, the micro- life of Chinese medicine after dispensing in sterilization time 20min, the S7
The storage temperature of state preparation is 4 DEG C.
Embodiment 2
A kind of Chinese medicine microecological preparation, including fermenting microbe, Chinese medicine compound prescription, nutriment composition and pure water, it is described
Fermenting microbe is lactic acid bacteria, and the inoculum concentration of the lactic acid bacteria is the 3% of inoculum volume, and the Chinese medicine compound prescription is according to quality component
Count as follows: 10 parts of Radix Astragali, 20 parts of dandelion, 20 parts of folium isatidis, the nutriment composition include glucose, yeast powder, egg
White peptone.
The lactic acid bacteria is Lactobacillus salivarius, and the viable count of the Lactobacillus salivarius is greater than or equal to 2 × 109Cfu/mL,
The Lactobacillus salivarius is the bacterial strain that deposit number is CGMCC No.14458.
The present invention also provides a kind of preparation methods of Chinese medicine microecological preparation, comprising the following steps:
S1, Radix Astragali, dandelion and folium isatidis are weighed according to suitable quality proportioning；
S2, Radix Astragali weighed in S1 is cut into blocky, dandelion and after folium isatidis is cut into strip, then by block-like Radix Astragali
And strip dandelion and folium isatidis be uniformly mixed, obtain Chinese medicine compound prescription；
S3, Chinese medicine compound prescription obtained in S2 is put into Chinese medicine decocting device, pure water is added, water is decocted 3 times, filtered out respectively
3 filtrates are merged and are concentrated into the medical fluid that raw drug content is 0.5g/mL by filtrate；
S4,5g glucose is added with every hydrargyrum oxydatum crudum liquid, 3g yeast powder and 3g peptone weigh suitable glucose, yeast powder and
Weighed glucose, yeast powder and peptone are obtained nutriment composition by peptone after mixing；
S5, nutriment composition obtained in S4 is added in medical fluid obtained in S3, is uniformly mixed, then benefit
PH value is adjusted to 7.0 with PH regulator, obtains culture solution after sterilized, natural cooling；
S6, lactic acid bacteria is accessed in culture solution with the inoculum concentration of nutrient solution volume 3% obtained in S4, under the conditions of 37 DEG C
Ferment 72h after to get arrive Chinese medicine microecological preparation；
S7, preservation will be put in storage after the packing of Chinese medicine microecological preparation obtained in S5.
The length of block-like Radix Astragali and wide no more than 1 ㎝, the dandelion of the strip and the length of folium isatidis in the S2
No more than 2 ㎝, Chinese medicine decocting device is the double boiler thermally conductive by heat medium in the S3, and water decocts specific in the S3
Method are as follows: the pure water that 4 times of volumes are added in Chinese medicine compound prescription carries out first time decoction, and decocting time 50min is filtered, filter
The pure water that 3 times of volumes are added in slag carries out second and decocts, decocting time 50min, filtering, adds 3 times of bodies in filter residue
Long-pending pure water carries out third time decoction, and decocting time 50min is filtered, the Chinese medicine compound prescription in the S3 is impregnated before decoction
25min, soaking temperature are 30 DEG C, and the speed of agitator being stirred in the S4 is 300r/min, and whipping temp is 35 DEG C, described
Sterilizing methods are 121 DEG C of autoclaving in S5, the micro- life of Chinese medicine after dispensing in sterilization time 25min, the S7
The storage temperature of state preparation is 4 DEG C.
Embodiment 3
A kind of Chinese medicine microecological preparation, including fermenting microbe, Chinese medicine compound prescription, nutriment composition and pure water, it is described
Fermenting microbe is lactic acid bacteria, and the inoculum concentration of the lactic acid bacteria is the 3% of inoculum volume, and the Chinese medicine compound prescription is according to quality component
Count as follows: 15 parts of Radix Astragali, 30 parts of dandelion, 30 parts of folium isatidis, the nutriment composition include glucose, yeast powder, egg
White peptone.
The lactic acid bacteria is Lactobacillus salivarius, and the viable count of the Lactobacillus salivarius is greater than or equal to 2.5 × 109cfu/
ML, the Lactobacillus salivarius are the bacterial strain that deposit number is CGMCC No.14458.
The present invention also provides a kind of preparation methods of Chinese medicine microecological preparation, comprising the following steps:
S1, Radix Astragali, dandelion and folium isatidis are weighed according to suitable quality proportioning；
S2, Radix Astragali weighed in S1 is cut into blocky, dandelion and after folium isatidis is cut into strip, then by block-like Radix Astragali
And strip dandelion and folium isatidis be uniformly mixed, obtain Chinese medicine compound prescription；
S3, Chinese medicine compound prescription obtained in S2 is put into Chinese medicine decocting device, pure water is added, water is decocted 3 times, filtered out respectively
3 filtrates are merged and are concentrated into the medical fluid that raw drug content is 0.5g/mL by filtrate；
S4,5g glucose is added with every hydrargyrum oxydatum crudum liquid, 3g yeast powder and 3g peptone weigh suitable glucose, yeast powder and
Weighed glucose, yeast powder and peptone are obtained nutriment composition by peptone after mixing；
S5, nutriment composition obtained in S4 is added in medical fluid obtained in S3, is uniformly mixed, then benefit
PH value is adjusted to 7.0 with PH regulator, obtains culture solution after sterilized, natural cooling；
S6, lactic acid bacteria is accessed in culture solution with the inoculum concentration of nutrient solution volume 3% obtained in S4, under the conditions of 37 DEG C
Ferment 84h after to get arrive Chinese medicine microecological preparation；
S7, preservation will be put in storage after the packing of Chinese medicine microecological preparation obtained in S5.
The length of block-like Radix Astragali and wide no more than 1 ㎝, the dandelion of the strip and the length of folium isatidis in the S2
No more than 2 ㎝, Chinese medicine decocting device is the double boiler thermally conductive by heat medium in the S3, and water decocts specific in the S3
Method are as follows: the pure water that 4 times of volumes are added in Chinese medicine compound prescription carries out first time decoction, and decocting time 60min is filtered, filter
The pure water that 3 times of volumes are added in slag carries out second and decocts, decocting time 60min, filtering, adds 3 times of bodies in filter residue
Long-pending pure water carries out third time decoction, and decocting time 60min is filtered, the Chinese medicine compound prescription in the S3 is impregnated before decoction
30min, soaking temperature are 35 DEG C, and the speed of agitator being stirred in the S4 is 400r/min, and whipping temp is 45 DEG C, described
Sterilizing methods are 121 DEG C of autoclaving in S5, the micro- life of Chinese medicine after dispensing in sterilization time 30min, the S7
The storage temperature of state preparation is 4 DEG C.
In summary: a kind of Chinese medicine microecological preparation composition proportion provided by the invention is reasonable, under instruction of Chinese Medicine theory,
Using Chinese medicine as fermentation raw material, bioconversion is carried out to intermediate material using preferred lactic acid bacteria, nontoxic, green, easy suction is made
Receive, the Chinese medicine microecological preparation that is remarkably reinforced of antibacterial activity, make drug effect be improved significantly, a kind of Chinese medicine provided by the invention is micro-
The preparation method advantages of simple of ecological agent, ferments to Chinese medicine, so that fermented tcm combines traditional Chinese medicine and probiotics
The advantages of, and it is with short production cycle, cost is relatively low.
Sow to be produced similar in 50 figures is chosen by the Chinese medicine microecological preparation through being used as examination in external injection birth canal
Group is tested, in addition chooses 50 with the similar sow to be produced of test group figure for the physiology with the Chinese medicine microecological preparation isodose
Salt water through as a control group, finding that sow lochia discharge duration test group is more flat than control group in external injection birth canal by contrast
Every shortening 1.27d (P < 0.05), 3 more than control group, weaned piglet retries postpartum insemination of sows success head number test group
Group is tested to be averaged every increase 0.32kg than control group.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of Chinese medicine microecological preparation, which is characterized in that including fermenting microbe, Chinese medicine compound prescription, nutriment composition and pure
Water purification；
The fermenting microbe is lactic acid bacteria, and the inoculum concentration of the lactic acid bacteria is the 3% of inoculum volume；
The Chinese medicine compound prescription is as follows according to quality component meter: 5~15 parts of Radix Astragali, 10~30 parts of dandelion, 10~30 parts of folium isatidis；
The nutriment composition includes glucose, yeast powder, peptone.
2. a kind of Chinese medicine microecological preparation according to claim 1, it is characterised in that: the lactic acid bacteria is saliva cream bar
The viable count of bacterium, the Lactobacillus salivarius is greater than or equal to (1.2~2.5) × 109Cfu/mL, the Lactobacillus salivarius are preservation
Number is the bacterial strain of CGMCC No.14458.
3. a kind of preparation method of Chinese medicine microecological preparation as claimed in claim 1 or 2, which is characterized in that including following step
It is rapid:
S1, Radix Astragali, dandelion and folium isatidis are weighed according to suitable quality proportioning；
S2, Radix Astragali weighed in S1 is cut into blocky, dandelion and after folium isatidis is cut into strip, then by block-like Radix Astragali and
The dandelion and folium isatidis of strip are uniformly mixed, and obtain Chinese medicine compound prescription；
S3, Chinese medicine compound prescription obtained in S2 is put into Chinese medicine decocting device, pure water is added, water decocts 3 times, filters out filter respectively
3 filtrates are merged and are concentrated into the medical fluid that raw drug content is 0.5g/mL by liquid；
S4, suitable glucose, yeast powder and albumen are weighed with every hydrargyrum oxydatum crudum liquid addition 5g glucose, 3g yeast powder and 3g peptone
Weighed glucose, yeast powder and peptone are obtained nutriment composition by peptone after mixing；
S5, nutriment composition obtained in S4 is added in medical fluid obtained in S3, is uniformly mixed, recycle PH
Regulator adjusts pH value to 7.0, obtains culture solution after sterilized, natural cooling；
S6, lactic acid bacteria is accessed in culture solution with the inoculum concentration of nutrient solution volume 3% obtained in S4, ferments under the conditions of 37 DEG C
After 60~84h to get arrive Chinese medicine microecological preparation；
S7, preservation will be put in storage after the packing of Chinese medicine microecological preparation obtained in S5.
4. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: blocky in the S2
The length of Radix Astragali and wide no more than 1 ㎝, the dandelion of the strip and the length of folium isatidis are no more than 2 ㎝.
5. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: Chinese medicine in the S3
Decocting device is the double boiler thermally conductive by heat medium.
6. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: decocting in the S3
It boils method particularly includes:
The pure water that 4 times of volumes are added in Chinese medicine compound prescription carries out first time decoction, and decocting time is 40~60min, filtering；
The pure water that 3 times of volumes are added in filter residue carries out second and decocts, and decocting time is 40~60min, filtering；
The pure water that 3 times of volumes are added in filter residue carries out third time decoction, and decocting time is 40~60min, filtering.
7. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: in the S3
Medicine compound impregnates 20~30min before decoction, and soaking temperature is 25~35 DEG C.
8. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: stirred in the S4
Mixed speed of agitator is 200~400r/min, and whipping temp is 25~45 DEG C.
9. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: sterilize in the S5
The autoclaving that method is 121 DEG C, sterilization time are 20~30min.
10. a kind of preparation method of Chinese medicine microecological preparation according to claim 3, it is characterised in that: divide in the S7
The storage temperature of Chinese medicine microecological preparation after dress is 4 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110090284A (en) * | 2019-05-13 | 2019-08-06 | 北京南亚医学研究院 | A kind of Chinese medicine microecological preparation and its preparation method and application |
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| CN115006474B (en) * | 2021-03-03 | 2024-02-23 | 洛阳东医堂药业有限公司 | Traditional Chinese medicine compatibility antibacterial drug |
| CN116898921A (en) * | 2023-08-10 | 2023-10-20 | 江西新世纪民星动物保健品有限公司 | Preparation method of cattail and dyer woad compound traditional Chinese medicine preparation |
| CN116898921B (en) * | 2023-08-10 | 2024-05-31 | 江西新世纪民星动物保健品有限公司 | Preparation method of cattail and dyer woad compound traditional Chinese medicine preparation |
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