CN111000905A - Traditional Chinese medicine composition for treating dysentery by using milk, solid fermentation traditional Chinese medicine preparation and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating dysentery by using milk, solid fermentation traditional Chinese medicine preparation and preparation method thereof Download PDFInfo
- Publication number
- CN111000905A CN111000905A CN201911358940.7A CN201911358940A CN111000905A CN 111000905 A CN111000905 A CN 111000905A CN 201911358940 A CN201911358940 A CN 201911358940A CN 111000905 A CN111000905 A CN 111000905A
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese medicine
- traditional chinese
- solid
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/12—Antidiarrhoeals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention provides a traditional Chinese medicine composition for treating dysentery through milk, a solid fermentation traditional Chinese medicine preparation and a preparation method thereof, wherein the composition comprises the following components: 35-40 parts of Chinese pulsatilla root, 30-35 parts of coptis root, 25-30 parts of ash bark, 25-30 parts of codonopsis pilosula, 25-30 parts of bighead atractylodes rhizome, 22-28 parts of poria cocos, 22-28 parts of white hyacinth bean and 20-25 parts of liquorice. The traditional Chinese medicine preparation comprises a traditional Chinese medicine composition and probiotics, wherein the probiotics are composite bacteria of saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum, and the traditional Chinese medicine composition is fermented by the probiotics to prepare the solid-state fermentation traditional Chinese medicine preparation. The solid-state fermented Chinese medicinal preparation can be used for stopping dysentery by milk, and is added with feed for lactating sow to prevent diarrhea of piglets and maintain intestinal health.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a traditional Chinese medicine composition for treating dysentery through lactation, a solid fermentation traditional Chinese medicine preparation and a preparation method thereof.
Background
Most pig farms have diarrhea diseases with high mortality rate after birth (within 3-5 days of age) of suckling piglets, the piglets can be 1 day of age at the earliest, are generally 2-3 days of age, the course of disease is 3-5 days, and the mortality rate is 90 percent and can reach 100 percent at the highest. Some piglets may have vomiting before they start diarrhea, and the vomit is yellow-white milk curd. The diarrhea feces are yellow white loose stools, the diarrhea is severe with water sample, no specific foul smell exists, most sick pigs have normal body temperature, piglets are in heavy mental depression during the diarrhea process, milk is refused to eat, the diarrhea is quickly dehydrated after occurring, eyeball depression is obvious, and the sick pigs mostly die due to dehydration failure.
A large number of clinical practice traces show that the causes of diarrhea of suckling piglets are as follows:
⑴ diarrhea of piglet caused by mycotoxin, wherein when the sow is pregnant, a large amount of feed containing mycotoxin is eaten, toxin is accumulated in the sow to cause the liver of the pregnant sow to be damaged, the detoxifying function of the liver is almost lost, then the toxin is accumulated and precipitated in the liver of a newborn piglet to cause the hepatobiliary function of the piglet to be damaged, the liver lesion of the piglet during the autopsy indicates the damage of the liver, the damaged and denatured liver, the bile secretion is reduced or lost after the liver is hardened, the fat in milk can not be fully digested by the intestinal tract, 40% of the fat can not be emulsified and absorbed, and the fatty acid and monoglyceride generated by the decomposition of the milk fat by pancreatic lipase can not be dissolved and absorbed, the undigested milk fat is retained in the gastrointestinal tract, and the parietal cells can secrete excessive water in the intestinal tract to maintain the osmotic pressure inside and outside the intestinal tract and intestinal cells to cause the quick dehydration of the organism, and the diarrhea is defined as the hepatic diarrhea.
⑵ sow constipation is caused by toxin discharge obstruction in vivo, which causes breast edema of sow, various inflammations (such as metritis and mastitis) and toxin production, and no drinking after delivery, no milk or little milk, which causes diarrhea of piglets.
⑶ the feed is incomplete and insufficient in the late stage of pregnancy, the mammary gland is not fully developed, the nutrition is unbalanced, the energy is too high, the obesity and endocrine dyscrasia are caused, the piglet is not full of food, the resistance is reduced, the disease and diarrhea are easily infected, the piglet can suffer from diarrhea caused by no milk, too strong milk and too thin milk after delivery, the sow is not milk, the piglet can not eat the colostrum in time to obtain maternal antibodies, the milk is too strong, the fat and protein are too high, the piglet is easy to digest badly, the milk is too thin, the nutrition is not full, and some mineral substances, vitamins and other nutritional factors are lacked.
⑷ the sow has low feed intake, insufficient energy supply or incomplete nutrition intake during lactation period, which causes the lactation sow to lose weight, the sow uses fat in the body to induce milk, the milk has too much long chain fatty acid, the suckling piglet sucks the milk which has insufficient nutrition, excessive fat and can not digest and absorb the milk, thus causing nutritive diarrhea.
⑸ when sow is infected with blue ear disease, swine fever, pseudorabies, eperythrozoon, etc., there are many inflammatory products in sow, so there are many inflammatory factors in milk, and piglet is poisoned after sucking milk, resulting in nonpathogenic diarrhea of suckling piglet.
According to the cause of diarrhea of the suckling piglets, the following measures are generally taken for prevention and treatment:
firstly, strict feed quality is guaranteed, the feed does not need to be mildewed, and particularly, the mildewed corns cannot be used; secondly, immunization of viral diseases such as sow swine fever, pseudorabies, porcine reproductive and respiratory syndrome, parvovirus and the like is well performed; epidemic diarrhea and transmissible gastroenteritis of swine bivalent inactivated vaccine (nest-alternating injection) are immunized with fetus, and are immunized 1 time and 4 mL/head respectively at 60d and 90d after pregnancy. The level of maternal antibodies is improved before the delivery of the sow; thirdly, increasing the daily ration crude fiber of the pregnant sow, limiting the feeding by 6 to 8 percent and freely eating by 12 to 15 percent; fourthly, the nutrition of digestible amino acid, vitamin, trace element, mineral substance and the like in the feed for the lactating sows is strengthened; adding microecological preparation (Bacillus, lactobacillus, active yeast, Bacillus bifidus, etc.) and complex enzyme to control mycotoxin; the piglets do not need to be urgently subjected to painful and infectious operations such as tooth cutting, tail breaking, castration and the like during birth, and the piglets need to suck colostrum and eat sufficient colostrum as soon as possible within 30min after delivery.
The prevention and treatment measures reduce the occurrence of diarrhea of the suckling piglets to a certain extent. However, the effect is increasingly poor due to deterioration of the breeding environment and variation of virus diseases of the breeding sows.
Disclosure of Invention
In order to solve the problem of poor prevention and treatment measures for diarrhea of suckling piglets, the invention provides a traditional Chinese medicine composition for stopping diarrhea after milk is passed, and provides a solid-state fermentation traditional Chinese medicine preparation for stopping diarrhea after milk is passed on the basis of the traditional Chinese medicine composition.
The technical scheme of the invention is realized as follows:
a traditional Chinese medicine composition for treating dysentery through milk is prepared from the following raw materials in parts by weight:
35-40 parts of Chinese pulsatilla root, 30-35 parts of coptis root, 25-30 parts of ash bark, 25-30 parts of codonopsis pilosula, 25-30 parts of bighead atractylodes rhizome, 22-28 parts of poria cocos, 22-28 parts of white hyacinth bean and 20-25 parts of liquorice.
Preferably, the feed additive is prepared from the following raw materials in parts by weight:
35 parts of Chinese pulsatilla root, 30 parts of coptis root, 25 parts of ash bark, 25 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 22 parts of poria cocos, 22 parts of white hyacinth bean and 20 parts of liquorice.
A solid-state fermentation traditional Chinese medicine preparation for treating dysentery by passing milk comprises the following raw materials:
the traditional Chinese medicine composition for treating dysentery through milk and the probiotics are disclosed, wherein the probiotics are composite bacteria of saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum.
Preferably, the species of the probiotic is a secondary seed liquid.
The invention also aims to provide a preparation method of the solid fermentation traditional Chinese medicine preparation for treating dysentery by passing milk, which comprises the following steps:
1) weighing the Chinese pulsatilla root, the coptis root, the ash bark, the codonopsis pilosula, the bighead atractylodes rhizome, the poria cocos, the white hyacinth bean and the liquorice according to the weight parts, mixing and crushing to obtain mixed powder;
2) adding the mixed powder obtained in the step 1) into a solid fermentation culture medium, uniformly mixing to obtain a traditional Chinese medicine solid fermentation culture medium, inoculating probiotics, fermenting at 36-38 ℃ for 24-48 h, stopping fermentation, and spray drying the fermented product to obtain the solid fermentation traditional Chinese medicine preparation.
Preferably, the adding ratio of the mixed powder to the solid fermentation medium is (8-20) g: 1L.
Preferably, the formula of the solid state fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
Preferably, the species of the probiotic is a secondary seed liquid.
Preferably, the secondary seed liquid is prepared by the following method:
activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12-24 h at 36-38 ℃ to obtain a primary seed solution; inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and performing static culture at 36-38 ℃ for 12-24 h to obtain a secondary seed solution.
Preferably, the seed culture medium of the primary seed liquid is the same as that of the secondary seed liquid, and the formula is as follows: 10 g of refined peptone, 5 g of yeast extract, 20g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 800.1 g of tween, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
The invention has the beneficial effects that:
1. the traditional Chinese medicine composition provided by the invention has the advantages that the glucoside, saponin, aglycone, flavone, terpenoid, sterol and the like in the effective components of the traditional Chinese medicine have biological activity and can promote and amplify the antibacterial and immune effects of the traditional Chinese medicine through the fermentation of the composite bacteria of saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum. The macromolecular effective components become micromolecular effective components which are easier to digest and absorb, and the drug property is improved. Meanwhile, the compound microecological preparation is the most loyal and sanitary for maintaining intestinal health, and the problem of diarrhea of piglets which is complicated can be quickly solved.
2. In the formula, the Chinese pulsatilla root, which is bitter and cold, mainly enters large intestine channels, clears heat and detoxifies, cools blood and stops dysentery, is the essential drug for treating heat-toxicity and bloody dysentery, so the Chinese pulsatilla root, which is a monarch drug. Coptis root, rhizoma Coptidis, being bitter and cold in property, has the effects of clearing heat and toxic materials, eliminating dampness and stopping dysentery, and is used as a ministerial drug to strengthen the action of the monarch drug. The ash bark, bitter and astringent, is used as an adjuvant drug for entering the large intestine channel to clear heat and dry dampness, astringe and stop dysentery. Licorice root, radix Glycyrrhizae strengthens the stomach and regulates the middle warmer, and is used as adjuvant to eliminate pathogens and strengthen healthy qi. Dang Shen is sweet and neutral. It enters spleen and lung meridians. Has the main effects of tonifying spleen and lung, nourishing blood and promoting the production of body fluid. It is combined with Bai Zhu and Fu Ling to tonify qi, invigorate spleen and remove dampness. The medicines are combined to play the roles of clearing heat and removing toxicity, cooling blood and treating dysentery.
3. Bai Dou is sweet and warm. It enters spleen and stomach meridians. Invigorating spleen and regulating stomach, eliminating dampness and relieving summer heat; can be used for treating weakness of spleen and stomach, anorexia, vomiting and diarrhea due to summer-heat and dampness, chest distress, and abdominal distention. Lentils contain viral inhibitory components, both in the water-soluble high molecular and low molecular weight fractions, which are effective in inhibiting viral growth.
4. The solid state fermentation traditional Chinese medicine preparation for treating diarrhea by passing milk of the invention prevents and controls diarrhea mechanism of suckling piglets: various toxins (mycotoxin, medicinal toxin, internal heat toxin and raw material toxin) exist in the body of the nursing sow, and the immune organ is damaged, so that five-organ disharmony, imbalance of yin and yang and rapid decline of immunity are caused. In sub-healthy lactating sows, the diarrhea of the lactating piglets is induced. After the solid-state fermentation traditional Chinese medicine preparation is added into the feed for the lactating sows, the toxin in the lactating sows can be effectively removed, the lactating sows are promoted to be recovered, high-quality milk is supplied to the lactating piglets, and the piglets eat the traditional Chinese medicine preparation through the lactation, so that the problem of diarrhea of the piglets is solved.
Detailed Description
The technical solution of the present invention is further described in detail below:
the invention relates to a traditional Chinese medicine composition for young poultry, which is prepared from the following raw materials in parts by weight:
35-40 parts of Chinese pulsatilla root, 30-35 parts of coptis root, 25-30 parts of ash bark, 25-30 parts of codonopsis pilosula, 25-30 parts of bighead atractylodes rhizome, 22-28 parts of poria cocos, 22-28 parts of white hyacinth bean and 20-25 parts of liquorice.
The coptis root is also named as Chuan Lian, Chuan Lian Huang Lian, ginger processed coptis root, ginger Lian, Yu Lian, etc. It is recorded in Shen nong Ben Cao Jing and listed as the top grade. The main components of coptis chinensis are Berberine (Berberine), Coptisine (Coptisine), Palmatine (Palmatine) with different names of Palmatine, Magnoline (Magnoline), 2,3, 4-trihydroxy phenylpropyl, 3-carboxyl-4-hydroxyphenoxy glucose, 3, 4-dihydroxyphenylethanol glucoside and the like. The coptis has a plurality of functions, and has the functions of resisting fungi, expelling toxin and removing toxin.
Pulsatilla, a perennial herb of the genus anemone of the family Ranunculaceae, grows with rhizomes, leaves are ovoid, and calyx is bluish purple. Other names are Neem grass, common Mesona herb, white cephalanoplos herb, Nibeau sagina herb and the like. Trichomonas vaginalis resistance, oxidation resistance and inflammation resistance: yao D et al found that Pulsatilla chinensis has effects of preventing peroxide release in liver tissue, increasing MnSOD activity, and preventing hepatitis B virus infection. Killing insects and inhibiting bacteria: the research of Lanyu and the like finds that the Chinese pulsatilla root decoction has better effect of killing the entamoeba histolytica. The Chinese pulsatilla root water extract has obvious inhibiting effect on staphylococcus aureus, pseudomonas aeruginosa, bacillus anthracis, typhoid bacillus, streptococcus A and streptococcus B through K-B paper diffusion method.
Cortex Fraxini, the name of Chinese medicine. Is dry branch bark or dry bark of Carpinus laxiflora, Fraxinus rhynchophylla, Fraxinus pinnata or Fraxinus pinnitafida of Oleaceae. Compendium of materia Medica: "Qin Pi" is used for eye disease, Jing Epilepsy, it is used for treating dysentery with metrorrhagia and leukorrhagia, and it is also used for astringing. It is also indicated for male oligospermia with astringent and tonifying effects. It is considered that the herbs are good at treating eye diseases such as fright, Epilepsy, metrorrhagia and dysentery, but some herbs are discarded and good at the head.
Yeast cell wall uses the chemical structure of glucose mannan in yeast wall, and has affinity with mycotoxin belonging to organic class to adsorb mycotoxin, and the yeast polysaccharide is an immunopotentiator capable of exciting nonspecific and specific immune reaction, activating T cell, B cell, macrophage and Natural Killer (NK) cell, and raising nonspecific disease-resisting capability of animal, so that it can raise animal production property.
Example 1
A traditional Chinese medicine composition for treating dysentery through milk is prepared from the following raw materials in parts by weight:
35 parts of Chinese pulsatilla root, 30 parts of coptis root, 25 parts of ash bark, 25 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 22 parts of poria cocos, 22 parts of white hyacinth bean and 20 parts of liquorice.
Example 2
A traditional Chinese medicine composition for treating dysentery through milk is prepared from the following raw materials in parts by weight:
40 parts of Chinese pulsatilla root, 32 parts of coptis root, 28 parts of ash bark, 30 parts of codonopsis pilosula, 30 parts of bighead atractylodes rhizome, 25 parts of poria cocos, 26 parts of white hyacinth bean and 25 parts of liquorice.
Example 3
A traditional Chinese medicine composition for treating dysentery through milk is prepared from the following raw materials in parts by weight:
36 parts of Chinese pulsatilla root, 35 parts of coptis root, 30 parts of ash bark, 27 parts of codonopsis pilosula, 28 parts of bighead atractylodes rhizome, 28 parts of poria cocos, 22 parts of white hyacinth bean and 23 parts of liquorice.
Example 4
A traditional Chinese medicine composition for treating dysentery through milk is prepared from the following raw materials in parts by weight:
38 parts of Chinese pulsatilla root, 30 parts of coptis root, 27 parts of ash bark, 30 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 24 parts of poria cocos, 28 parts of white hyacinth bean and 25 parts of liquorice.
Example 5
A solid-state fermentation traditional Chinese medicine preparation for treating dysentery by passing milk comprises the following raw materials:
the traditional Chinese medicine composition for treating dysentery through milk and the probiotics in the embodiment 1 are the composite bacteria of saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum. The strain of the probiotics is a secondary seed liquid.
The preparation method comprises the following steps:
1) weighing the Chinese pulsatilla root, the coptis root, the ash bark, the codonopsis pilosula, the bighead atractylodes rhizome, the poria cocos, the white hyacinth bean and the liquorice according to the weight parts, mixing and crushing to obtain mixed powder;
2) adding the mixed powder obtained in the step 1) into a solid fermentation culture medium, uniformly mixing to obtain a traditional Chinese medicine solid fermentation culture medium, inoculating probiotics, fermenting at 36 ℃ for 48h, stopping fermentation, and spray drying the fermented product to obtain the solid fermentation traditional Chinese medicine preparation. The adding ratio of the mixed powder to the solid fermentation medium is 20g to 1L.
The formula of the solid fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
The secondary seed liquid is prepared by the following method:
activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing at 38 ℃ for 12 hours to obtain a primary seed solution; inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2 vol%, and performing static culture at 36 ℃ for 24h to obtain a secondary seed solution.
The seed culture medium of the first-stage seed liquid is the same as that of the second-stage seed liquid, and the formula is as follows: 10 g of refined peptone, 5 g of yeast extract, 20g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 800.1 g of tween, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Example 6
A solid-state fermentation traditional Chinese medicine preparation for treating dysentery by passing milk comprises the following raw materials:
the traditional Chinese medicine composition for treating dysentery through milk and the probiotics in the embodiment 1 are the composite bacteria of saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum. The strain of the probiotics is a secondary seed liquid.
The preparation method comprises the following steps:
1) weighing the Chinese pulsatilla root, the coptis root, the ash bark, the codonopsis pilosula, the bighead atractylodes rhizome, the poria cocos, the white hyacinth bean and the liquorice according to the weight parts, mixing and crushing to obtain mixed powder;
2) adding the mixed powder obtained in the step 1) into a solid fermentation culture medium, uniformly mixing to obtain a traditional Chinese medicine solid fermentation culture medium, inoculating probiotics, fermenting at 36 ℃ for 48h, stopping fermentation, and spray drying the fermented product to obtain the solid fermentation traditional Chinese medicine preparation. The adding ratio of the mixed powder to the solid fermentation medium is 8g to 1L.
The formula of the solid fermentation medium is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
The secondary seed liquid is prepared by the following method:
activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 24 hours at 36 ℃ to obtain a primary seed solution; inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 10 vol%, and performing static culture at 38 ℃ for 12h to obtain a secondary seed solution.
The seed culture medium of the first-stage seed liquid is the same as that of the second-stage seed liquid, and the formula is as follows: refined peptone 10 g, yeast extract 5 g, glucose 20g, sodium acetate 5 g, ammonium citrate 2 g, Tween 800.1 g, magnesium sulfate 0.58 g, manganese sulfate 0.28 g and distilled water 1000ml
Test examples
In 2019, a certain pig farm in Sulan city, Jilin province, 8 months, was tested, and 45 sows were housed, among which 30 pregnant sows were present. 30 pregnant sows are divided into 3 groups, 1 group of drinking water is added with the solid state fermentation traditional Chinese medicine preparation for stopping diarrhea after passing milk of the invention example 5 (the whole material of the pregnant sows is added with 1000g/T of the product, the first dosage is doubled after 10 days), 2 groups of drinking water is added with the solid state fermentation traditional Chinese medicine preparation for stopping diarrhea after passing milk of the invention example 6 (the whole material of the pregnant sows is added with 1000g/T of the product, the first dosage is doubled after 10 days), and the 3 rd group is a control group (the drinking water is added with antibiotics regularly according to the conventional health care).
By the end of 9 months in 2019, each group of pregnant sows was tested for successive farrowing. Some diarrhea and death of the suckling piglets appear in each group. The number of piglets with diarrhea and dead piglets in each group is shown in table 1.
TABLE 1 piglet diarrhea test
In the test process, the diarrhea incidence rates of the suckling piglets of the test 1 group and the test 2 group are 4.5 percent and 4.8 percent respectively, while the diarrhea incidence rate of the suckling piglets of the control group is 12 percent, the diarrhea incidence rate of the suckling piglets of each test group is lower than that of the control group, and the difference is obvious; at the end of the test (statistical data of piglets at 30-day age), the death and elimination rate of each test group is 1.8% and 1.9%, the death and elimination rate of the control group is 10.4%, and the death and elimination rate of each test group is lower than that of the control group (the difference is significant).
The solid-state fermentation traditional Chinese medicine preparation for treating the dysentery through the milk provided by the invention has the advantages that the traditional Chinese medicine is used as a fermentation raw material, the optimized saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum are used for carrying out biotransformation on intermediate substances to prepare the traditional Chinese medicine microecological preparation which is non-toxic, green, easy to absorb and obviously enhanced in antibacterial activity, so that the medicine effect is obviously improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The traditional Chinese medicine composition for treating dysentery through milk is characterized by being prepared from the following raw materials in parts by weight:
35-40 parts of Chinese pulsatilla root, 30-35 parts of coptis root, 25-30 parts of ash bark, 25-30 parts of codonopsis pilosula, 25-30 parts of bighead atractylodes rhizome, 22-28 parts of poria cocos, 22-28 parts of white hyacinth bean and 20-25 parts of liquorice.
2. The traditional Chinese medicine composition for treating dysentery through milk according to claim 1, which is prepared from the following raw materials in parts by weight:
35 parts of Chinese pulsatilla root, 30 parts of coptis root, 25 parts of ash bark, 25 parts of codonopsis pilosula, 25 parts of bighead atractylodes rhizome, 22 parts of poria cocos, 22 parts of white hyacinth bean and 20 parts of liquorice.
3. The solid-state fermentation traditional Chinese medicine preparation for treating dysentery according to claim 1, which comprises the following raw materials:
the composition of claim 1, wherein the probiotic is a complex of Saccharomyces cerevisiae, Bacillus subtilis, and Clostridium butyricum.
4. The solid-state fermented traditional Chinese medicine preparation for treating dysentery according to claim 3, wherein the strain of probiotic bacteria is secondary seed liquid.
5. The preparation method of the solid fermented traditional Chinese medicine preparation for treating dysentery according to claim 3, which comprises the following steps:
1) weighing the Chinese pulsatilla root, the coptis root, the ash bark, the codonopsis pilosula, the bighead atractylodes rhizome, the poria cocos, the white hyacinth bean and the liquorice according to the weight parts, mixing and crushing to obtain mixed powder;
2) adding the mixed powder obtained in the step 1) into a solid fermentation culture medium, uniformly mixing to obtain a traditional Chinese medicine solid fermentation culture medium, inoculating probiotics, fermenting at 36-38 ℃ for 24-48 h, stopping fermentation, and spray drying the fermented product to obtain the solid fermentation traditional Chinese medicine preparation.
6. The preparation method of the solid-state fermentation traditional Chinese medicine preparation for treating dysentery according to claim 5, wherein the adding ratio of the mixed powder to the solid-state fermentation culture medium is (8-20) g: 1L.
7. The method for preparing a solid-state fermentation Chinese medicinal preparation for treating dysentery according to claim 5, wherein the solid-state fermentation culture medium comprises: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5 g of glucose, 1 g of soluble starch, 5 g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water.
8. The method for preparing a solid state fermentation Chinese medicinal preparation for treating dysentery according to claim 5, wherein the strain of probiotic bacteria is secondary seed liquid.
9. The method for preparing the solid-state fermentation traditional Chinese medicine preparation for treating dysentery according to claim 5, wherein the secondary seed liquid is prepared by the following method:
activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12-24 h at 36-38 ℃ to obtain a primary seed solution; inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and performing static culture at 36-38 ℃ for 12-24 h to obtain a secondary seed solution.
10. The method for preparing a solid-state fermented Chinese medicinal preparation for treating dysentery according to claim 9, wherein the seed culture medium of the primary seed solution is the same as the seed culture medium of the secondary seed solution, and the formula of the solid-state fermented Chinese medicinal preparation is as follows: 10 g of refined peptone, 5 g of yeast extract, 20g of glucose, 5 g of sodium acetate, 2 g of ammonium citrate, 800.1 g of tween, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911358940.7A CN111000905A (en) | 2019-12-25 | 2019-12-25 | Traditional Chinese medicine composition for treating dysentery by using milk, solid fermentation traditional Chinese medicine preparation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911358940.7A CN111000905A (en) | 2019-12-25 | 2019-12-25 | Traditional Chinese medicine composition for treating dysentery by using milk, solid fermentation traditional Chinese medicine preparation and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111000905A true CN111000905A (en) | 2020-04-14 |
Family
ID=70118463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911358940.7A Withdrawn CN111000905A (en) | 2019-12-25 | 2019-12-25 | Traditional Chinese medicine composition for treating dysentery by using milk, solid fermentation traditional Chinese medicine preparation and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111000905A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114099590A (en) * | 2021-11-02 | 2022-03-01 | 江西新天地药业有限公司 | Fermented Chinese herbal medicine preparation for preventing and treating livestock and poultry diarrhea and preparation method thereof |
| CN115845004A (en) * | 2023-01-10 | 2023-03-28 | 河南大华生物技术有限公司 | A Chinese medicinal microcapsule for treating diarrhea in pig, and its preparation method |
-
2019
- 2019-12-25 CN CN201911358940.7A patent/CN111000905A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114099590A (en) * | 2021-11-02 | 2022-03-01 | 江西新天地药业有限公司 | Fermented Chinese herbal medicine preparation for preventing and treating livestock and poultry diarrhea and preparation method thereof |
| CN114099590B (en) * | 2021-11-02 | 2023-07-04 | 江西新天地药业有限公司 | Fermented Chinese herbal medicine preparation for preventing and treating diarrhea of livestock and poultry and preparation method thereof |
| CN115845004A (en) * | 2023-01-10 | 2023-03-28 | 河南大华生物技术有限公司 | A Chinese medicinal microcapsule for treating diarrhea in pig, and its preparation method |
| CN115845004B (en) * | 2023-01-10 | 2024-04-19 | 河南大华生物技术有限公司 | Traditional Chinese medicine microcapsule for treating pig diarrhea and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102835609B (en) | Pig feed capable of preventing piglets from diarrhea and traditional Chinese medicine composition | |
| CN101664421B (en) | Traditional Chinese biological preparation for preventing and treating livestock diarrhea and preparation method and feedstuff thereof | |
| CN102232487B (en) | Physiological regulator for maternal animals, and preparation method thereof | |
| CN102823779B (en) | Dairy cow feed, preparation method and traditional Chinese medicine for improving dairy cow production performance | |
| CN102940150B (en) | Chinese medicament for preventing and treating calf diarrhea and feed | |
| JP7201837B2 (en) | Composition for prevention, improvement or treatment of obesity or fatty liver disease containing Leuconostoc citreum WIKIM0104 | |
| CN106822890B (en) | Pharmaceutical composition for preventing and treating diarrhea of weaned piglets and preparation method and application thereof | |
| CN104920787B (en) | A kind of probiotics compound Chinese medicinal preparation for promoting sow lactation and improving milk water quality | |
| CN102805267B (en) | Feed for feeding broiler | |
| CN110506834A (en) | A method of cattle and sheep feed is produced using microbial fermentation | |
| CN105395676A (en) | Compound medicine composition for treating animal mycotoxicosis | |
| CN106581360A (en) | Probiotic fermented Chinese herbal medicine compound preparation for improving milk quality of sows and preparation method thereof | |
| CN101869224A (en) | Feed additive for improving lactation performance of lactating sows and preparation method thereof | |
| CN105614094A (en) | Fermented-feed raw material preparation special for pregnant sows and preparing method and application thereof | |
| CN104208240A (en) | Traditional Chinese herbal microecological preparation for preventing and treating piglet bacterial dysentery and preparation method thereof | |
| CN102526302A (en) | Chinese medicinal micro-ecological preparation for inhibiting piglet bacterial dysentery and preparation method thereof | |
| CN106798123A (en) | A kind of feed addictive for shortening sow stages of labor and improving Recovery | |
| CN111000905A (en) | Traditional Chinese medicine composition for treating dysentery by using milk, solid fermentation traditional Chinese medicine preparation and preparation method thereof | |
| Bąkowski et al. | Probiotic microorganisms and herbs in ruminant nutrition as natural modulators of health and production efficiency–A review | |
| CN114568602A (en) | Traditional Chinese medicine compound for improving growth performance and organism immunity of broiler chickens and preparation method and application thereof | |
| KR102012751B1 (en) | Platycodon grandiflorum extract animal feed additive and animal feed | |
| CN107348136A (en) | A kind of vegetalitas promotees feed addictive and the application of weanling pig gastro-intestinal digestion function | |
| CN102872321B (en) | Chinese medicinal preparation for preventing and controlling porcine reproductive and respiratory syndrome and preparation method thereof | |
| CN112426501A (en) | Traditional Chinese medicine microecological preparation for promoting growth and preventing diarrhea of calves and preparation method thereof | |
| CN104524453A (en) | Fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens and application of fermented Chinese herbal preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20200414 |