CN113945547B

CN113945547B - Multichannel LAMP detector and control method thereof

Info

Publication number: CN113945547B
Application number: CN202111141419.5A
Authority: CN
Inventors: 颜菁; 翟峰; 俞涛
Original assignee: Jiangsu Huixian Pharmaceutical Technology Co ltd
Current assignee: Jiangsu Huixian Pharmaceutical Technology Co ltd
Priority date: 2021-09-28
Filing date: 2021-09-28
Publication date: 2023-07-25
Anticipated expiration: 2041-09-28
Also published as: WO2023050710A1; CN113945547A

Abstract

The invention discloses a multichannel LAMP detector and a control method thereof. The detector comprises a chip bin and an optical detection mechanism, wherein the chip bin is provided with a plurality of chip slots; the optical detection mechanism can be movably arranged in the shell along the left-right direction and the front-back direction, and is positioned below the chip bin; each chip slot is provided with a plurality of detection sites which are arranged along the left-right direction, and each detection site of each chip slot corresponds to one detection position respectively; the multichannel LAMP detector further comprises: the X-direction driving mechanism is used for driving the optical detection mechanism to move along the left-right direction; the Y-direction driving mechanism is used for driving the optical detection mechanism to move along the front-back direction; and the controller is used for receiving detection signals of the X-direction starting point detection switch, the X-direction end point detection switch, the Y-direction starting point detection switch and the Y-direction end point detection switch and controlling the X-direction driving mechanism and the Y-direction driving mechanism. The invention can process samples in a plurality of microfluidic chips at one time and has smaller volume.

Description

Multichannel LAMP detector and control method thereof

Technical Field

The invention relates to a multichannel LAMP detector and a control method thereof.

Background

The LAMP (Loop-mediated isothermal amplification) technology is widely applied to the field of biological diagnosis due to the advantages of mild reaction conditions (lower reaction temperature), short reaction time and the like, such as nucleic acid amplification detection to diagnose whether pathogens exist in a sample. At present, the LAMP technology is a process of obtaining a nucleic acid amplification result by providing in vitro amplification conditions for a nucleic acid fragment, exponentially amplifying the nucleic acid fragment in large quantities, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and analyzing the fluorescent signal. In the nucleic acid amplification reaction, the reaction system needs to be heated. The existing LAMP detector can integrate nucleic acid amplification detection, and after a detection chip (usually a microfluidic chip) is placed in the LAMP detector, a reaction cavity (usually an amplification reaction cavity) of the detection chip can be heated, illuminated, detected and the like. When the sample size to be detected is large, there is a need for an LAMP detector capable of detecting a plurality of samples at once.

Disclosure of Invention

In view of the above-mentioned technical problems, an object of the present invention is to provide a multichannel LAMP detector that can process samples in a plurality of microfluidic chips at a time while being small in volume.

Another object of the present invention is to provide a control method of a multi-channel LAMP detector, which detects samples of a plurality of microfluidic chips at a time without increasing the cost of devices additionally.

According to a first aspect of the present invention, a multi-channel LAMP detector includes a chip bin and an optical detection mechanism, wherein the chip bin has a plurality of chip slots for accommodating microfluidic chips, and the plurality of chip slots are arranged in parallel along a front-rear direction; the optical detection mechanism is movably arranged in the shell along the left-right direction and the front-back direction, and is positioned below the chip bin; each chip slot is provided with a plurality of detection sites which are arranged along the left-right direction, the optical detection mechanism is provided with a plurality of detection positions, and each detection site of each chip slot corresponds to one detection position; the multichannel LAMP detector further comprises:

an X-direction driving mechanism for driving the optical detection mechanism to move in the left-right direction;

a Y-direction driving mechanism for driving the optical detection mechanism to move in the front-rear direction;

an X-direction start point detection switch for emitting a first detection signal when it is detected that the optical detection mechanism reaches its start point in the left-right direction;

An X-direction end point detection switch for emitting a second detection signal when it is detected that the optical detection mechanism reaches its end point in the left-right direction;

a Y-direction start point detection switch for detecting a third detection signal that the optical detection mechanism reaches its start point in the front-rear direction;

a Y-direction end point detection switch for detecting a fourth detection signal of the optical detection mechanism reaching an end point thereof in the front-rear direction;

and the controller is used for receiving detection signals of the X-direction starting point detection switch, the X-direction end point detection switch, the Y-direction starting point detection switch and the Y-direction end point detection switch, controlling the X-direction driving mechanism to operate in the forward direction after receiving the first detection signal, controlling the X-direction driving mechanism to operate in the reverse direction after receiving the second detection signal, controlling the Y-direction driving mechanism to operate in the forward direction after receiving the third detection signal and controlling the Y-direction driving mechanism to operate in the reverse direction after receiving the fourth detection signal.

In a preferred embodiment, the controller is configured to control, when the first detection signal and the third detection signal are received, the X-direction driving mechanism to operate in a forward direction for a first set distance, stop for a set time, repeat the process one or more times, and control the X-direction driving mechanism to operate in a reverse direction until the second detection signal is received; controlling the X-direction driving mechanism to stop running until the first detection signal is received again; and/or the number of the groups of groups,

The controller is further used for controlling the Y-direction driving mechanism to run forward for a second set distance and stopping the set time after receiving the first detection signal and the third detection signal; controlling the X-direction driving mechanism to run forward for a first set distance, stopping for a set time, repeating the process one or more times until the second detection signal is received, and controlling the X-direction driving mechanism to run reversely; controlling the X-direction driving mechanism to stop running until the first detection signal is received again; and/or

The controller is further used for controlling the Y-direction driving mechanism to operate positively when the first detection signal is received until the fourth detection signal is received, and controlling the Y-direction driving mechanism to stop operating; controlling the X-direction driving mechanism to run forward for a first set distance, stopping for a set time, repeating the process one or more times until the second detection signal is received, and controlling the X-direction driving mechanism to run reversely; controlling the X-direction driving mechanism to stop running until the first detection signal is received again; and/or the number of the groups of groups,

and the controller is also used for controlling the Y-direction driving mechanism to reversely operate when the fourth detection signal and the first detection signal are received until the third detection signal is received, and controlling the Y-direction driving mechanism to stop operating.

More preferably, the set times are equal.

In a preferred embodiment, the X-direction driving mechanism comprises an X-direction motor, the Y-direction driving mechanism comprises a Y-direction motor, and the controller is electrically connected with the X-direction motor and the Y-direction motor respectively; and/or the controller is respectively and electrically connected with the X-direction starting point detection switch, the X-direction end point detection switch, the Y-direction starting point detection switch and the Y-direction end point detection switch.

More preferably, the multi-channel LAMP detector includes a base having a plurality of X-direction guide rails arranged side by side and extending in a left-right direction; the multichannel LAMP detector further comprises a mounting block which is movably arranged on the X-direction guide rail along the left-right direction, wherein a Y-direction guide rail extending along the front-back direction is arranged on the mounting block; the optical detection mechanism is movably arranged on the Y-direction guide rail along the front-back direction; the X-direction motor is arranged on the base and is connected with the mounting block or the Y-direction guide rail through an X-direction screw rod, and the Y-direction motor is arranged on the mounting block and is connected with the optical detection mechanism through a Y-direction screw rod; the X-direction starting point detection switch and the X-direction end point detection switch are respectively close to two end parts of the X-direction guide rail, and the Y-direction starting point detection switch and the Y-direction end point detection switch are respectively close to two end parts of the Y-direction guide rail.

In a preferred embodiment, the multi-channel LAMP detector further includes a code scanner for reading a barcode on the microfluidic chip, and a display screen serving as a man-machine interaction interface, where the display screen has a first display state, and in the first display state, the display screen has a code scanning input button for a user to input a code scanning input instruction, and the controller is further configured to establish a correspondence between barcode information read by the code scanner and a corresponding detection channel according to a set order after receiving the code scanning input instruction, where each detection channel corresponds to one of the chip slots.

More preferably, the controller is further configured to receive detection data returned by the optical detection mechanism, and the display screen further has a second display state, where in the second display state, the display screen has a result display area corresponding to each detection site of each detection channel.

In a preferred embodiment, the multi-channel LAMP detector further comprises a housing, wherein a chip port for inserting a microfluidic chip is formed in the housing, and each chip slot is provided with a notch opposite to the chip port; the multi-channel LAMP detector further comprises heating components for heating the microfluidic chip in the chip slot at constant temperature, and at least one heating component is arranged on the side wall of each chip slot; the multichannel LAMP detector further comprises a mechanical arm mechanism, wherein the mechanical arm mechanism comprises a plurality of mechanical arms which can be connected with a piston of a microfluidic chip in the chip bin, each chip slot corresponds to at least one mechanical arm, and each mechanical arm can be movably arranged in the shell along the left-right direction and is positioned on the right side of the chip bin.

More preferably, the multi-channel LAMP detector further comprises a door for closing the chip port and a first driving mechanism for driving the door to move, wherein the door is movably arranged on the inner wall of the shell; and/or the multichannel LAMP detector further comprises a chip cover, wherein the chip cover is arranged between the inner wall of the shell surrounding the chip port and the chip bin and between the chip grooves.

More preferably, the mechanical arm mechanism comprises a bracket arranged on the inner wall of the shell, a lower mounting plate capable of being arranged on the bracket along the left-right direction and an upper mounting plate capable of being movably arranged on the bracket along the left-right direction, the upper mounting plate is positioned above the lower mounting plate, a plurality of first mechanical arms are arranged on the upper mounting plate, a plurality of second mechanical arms are arranged on the lower mounting plate, each chip slot corresponds to at least one first mechanical arm and at least one second mechanical arm respectively, and the second mechanical arms are positioned below the left of the first mechanical arms in the initial position.

Further, the first mechanical arm and/or the second mechanical arm are/is provided with a joint groove for the piston of the microfluidic chip to be clamped in, and the joint groove is provided with a notch facing upwards; and/or the mechanical arm mechanism further comprises a second driving mechanism for driving the upper mounting plate to move left and right and a third driving mechanism for driving the lower mounting plate to move left and right, the bracket is provided with a second guide rail and a third guide rail which extend along the left and right direction, the upper mounting plate is movably arranged on the second guide rail along the left and right direction, the lower mounting plate is movably arranged on the third guide rail along the left and right direction, the second driving mechanism comprises a second motor and a second screw rod which is driven to rotate by the second motor, the second screw rod is connected with the upper mounting plate, the third driving mechanism comprises a third motor and a third screw rod which is driven to rotate by the third motor, and the third screw rod is connected with the lower mounting plate; and/or the mechanical arm mechanism further comprises a first detection switch for detecting whether the first mechanical arm reaches the initial position of the mechanical arm and a second detection switch for detecting whether the second mechanical arm reaches the initial position of the mechanical arm; and/or the bracket has a blocking portion for preventing the upper and lower mounting plates from moving rightward beyond their set travel.

In a preferred embodiment, the multi-channel LAMP detector further comprises a main PCB board, a mounting frame located between the display screen and the chip bin is arranged in the housing, a guiding chute is arranged on the mounting frame, and an edge portion of the main PCB board is inserted into the guiding chute.

According to a second aspect of the present invention, a control method of a multi-channel LAMP detector as described above, the control method comprising the steps of:

s101, after receiving a first detection signal and a third detection signal, a controller judges that the optical detection mechanism is at an origin and detects a detection site P11 of a first microfluidic chip;

s102, the controller controls the X-direction driving mechanism to run forward, so that the optical detection mechanism moves rightwards by a set distance d1 and stops to a detection site P12 for detection; and so on until the detection of all detection sites of the first microfluidic chip is completed, at the moment, the X-direction end point detection switch is triggered to send out a second detection signal;

s103, after receiving the second detection signal, the controller controls the X-direction driving mechanism to reversely operate, so that the optical detection mechanism moves leftwards to return to the original point, and at the moment, the X-direction starting point detection switch is triggered to send out a first detection signal;

S104, after receiving the first detection signal and the third detection signal, the controller controls the Y-direction driving mechanism to move backwards by a set distance d2 and then stops to a detection site P21 of the second microfluidic chip for detection;

s105, the controller controls the X-direction driving mechanism to run forward, so that the optical detection mechanism moves rightwards by a set distance d1 and stops to a detection site P22 for detection; and the like until detection of all detection sites of the second microfluidic chip is completed, and at the moment, the X-direction end point detection switch is triggered to send out a second detection signal;

s106, after the controller receives the second detection signal, controlling the X-direction driving mechanism to reversely operate, so that the optical detection mechanism moves leftwards until the X-direction starting point detection switch is triggered to send out a first detection signal;

s107, after receiving the first detection signal, the controller controls the Y-direction driving mechanism to move backwards to a detection site Pn1 of the last microfluidic chip for detection;

s108, the controller controls the X-direction driving mechanism to run forward, so that the optical detection mechanism stops to a detection site Pn2 for detection after moving rightwards by a set distance d 1; and so on until the detection of all detection sites of the last microfluidic chip is completed, at the moment, the X-direction end point detection switch is triggered to send out a second detection signal;

S109, after the controller receives the second detection signal, the X-direction driving mechanism is controlled to reversely operate, so that the optical detection mechanism moves leftwards until the X-direction starting point detection switch is triggered to send out a first detection signal, and at the moment, the Y-direction end point detection switch is triggered to send out a fourth detection signal;

and S110, after the controller receives the first detection signal and the fourth detection signal, controlling the Y-direction driving mechanism to reversely operate, so that the optical detection mechanism moves forwards until the X-direction starting point detection switch and the Y-direction starting point detection switch are triggered to send out the first detection signal and the third detection signal, and stopping operation of the Y-direction driving mechanism.

In a preferred embodiment, the control method further comprises the steps of:

s201, receiving a first code scanning input instruction of a user through a display screen, reading first bar code information of a first microfluidic chip through a code scanner, and associating the first bar code information with a first detection channel after receiving the first code scanning input instruction and the first bar code information by a controller;

s202, receiving a second code scanning input instruction of a user through the display screen, reading second bar code information of a second microfluidic chip through the code scanner, and associating the second bar code information with a second detection channel after receiving the second code scanning input instruction and the second bar code information by the controller;

And the like, until all detection channels are correspondingly related to bar code information of the microfluidic chip;

in the step S101, the controller establishes a correspondence between the detection information sent by the optical detection mechanism and the detection site P11 of the first detection channel;

in step S102, the controller sequentially establishes a correspondence between the detection information sent by the optical detection mechanism and other detection sites of the first detection channel;

in step S104, the controller establishes a correspondence between the detection information sent by the optical detection mechanism and the detection site P21 of the second detection channel;

in step S105, the controller sequentially establishes a correspondence between the detection information sent by the optical detection mechanism and other detection sites of the second detection channel;

in the step S107, the controller establishes a correspondence between the detection information sent by the optical detection mechanism and the detection site Pn1 of the last detection channel;

in step S108, the controller sequentially establishes a correspondence between the detection information sent by the optical detection mechanism and other detection sites of the last detection channel.

Compared with the prior art, the invention has the following advantages:

According to the multichannel LAMP detector, the chip bin is internally provided with the plurality of chip slots, all detection sites of all detection channels are detected by controlling the optical detection mechanism to move in the front-back direction and the left-right direction, a plurality of microfluidic chips can be processed at one time, the number of samples which can be processed by one time of running a detection program is greatly increased, and higher detection efficiency is achieved; moreover, the optical detection mechanism can be shared by a plurality of detection channels of the LAMP detector, compared with a single-channel LAMP detector, the space is effectively utilized, the whole volume is not obviously increased, and the space is not additionally occupied.

Drawings

In order to more clearly illustrate the technical solutions of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic diagram of the overall structure of a multi-channel LAMP detector according to the present embodiment;

fig. 2 is a schematic diagram of the internal structure of a multi-channel LAMP detector according to the present embodiment;

Fig. 3 is a schematic diagram showing an internal structure of a multi-channel LAMP detector according to another view angle of the present embodiment;

fig. 4 is a bottom view of the inner wall of the housing of the multi-channel LAMP detector according to the present embodiment;

FIG. 5 is a schematic view of the structure of the code scanner frame;

FIG. 6 is a schematic view of another view of the scanner frame;

FIG. 7 is a schematic diagram of the structure of the first heating module and the second heating module;

FIG. 8 is a schematic diagram of a structure of a first heating module and a second heating module from another perspective;

FIG. 9 is a schematic diagram of a robotic arm mechanism;

FIG. 10 is another schematic view of a robotic arm mechanism;

FIG. 11a is an isometric view of an optical detection mechanism;

FIG. 11b is a top view of the optical detection mechanism;

FIG. 12 is a schematic structural view of the mounting bracket;

fig. 13 is a schematic structural diagram of a microfluidic chip;

FIG. 14 is a schematic diagram of a chip cover;

FIGS. 15a and 15b are schematic diagrams illustrating a chip slot, respectively;

FIG. 16 is a schematic diagram of a detection site.

Wherein, the liquid crystal display device comprises a liquid crystal display device,

1. a housing; 2. a chip bin; 3. a door; 4. a chip cover; 6. a heating assembly; 7. a mechanical arm mechanism; 8. an optical detection mechanism; 10. a chip slot; 11. a base; 12. a footing; 13. a connection hole;

100. a microfluidic chip; 100a, a piston; 100a1, neck;

101. A chip port; 102. a notch;

301. a first guide rail; 302. a first motor; 303. a first screw rod;

501. a code scanner; 502. a code scanner bracket; 5021. a base; 5022. a support table; 503. scanning a window; 504. a display screen;

601. a first heating module; 6011. a first heat-insulating cotton pressing block; 6012. a first heating block; 6013. a first heating film; 6014. a first heat insulating sheet; 6015. a first thermal insulation cotton; 602. a second heating module; 6021. a second heat-insulating cotton pressing block; 6022. a second heating block; 6023. a second heating film; 6024. a second heat insulating sheet; 6025. a second heat-insulating cotton; 603. a first temperature sensor; 604. a second temperature sensor;

701. a bracket; 7011. a blocking portion; 702. an upper mounting plate; 703. a lower mounting plate; 704. a first mechanical arm; 7041. the first photoelectric switch induction bracket; 705. a second mechanical arm; 7051. the second photoelectric switch induction bracket; 706. an engagement groove; 7071. a second motor; 7072. a second screw rod; 7081. a third motor; 709. a second guide rail; 711. a first photoelectric switch; 712. a second photoelectric switch;

801. an X-direction starting point detection switch; 802. an X-direction end point detection switch; 803. y-direction starting point detection switch; 804. y-direction end point detection switch; 805. an X-direction motor; 806. a Y-direction motor; 809. an X-direction guide rail; 810. a Y-direction guide rail;

91. A main PCB board; 92. the mechanical arm controls the PCB; 93. a door control PCB;

101. a mounting frame; 101a, guiding chute.

Detailed Description

Preferred embodiments of the present invention will be described in detail below with reference to the attached drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention.

In the description of the present application, it should be understood that the terms "upper," "lower," "left," "right," "front," "rear," "vertical," "horizontal," "inner," and "outer," etc. indicate orientations or positional relationships based on the orientations or positional relationships illustrated in fig. 1 and 2, merely for purposes of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements being referred to must have a particular orientation, be configured and operated in a particular orientation, and are not to be construed as limiting the present application.

As used in this specification and in the claims, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus. The term "and/or" as used herein includes any combination of one or more of the associated listed items.

It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly or indirectly fixed or connected to the other feature. Further, the descriptions of the upper, lower, left, right, etc. used in the present invention are merely with respect to the mutual positional relationship of the constituent elements of the present invention in the drawings.

It is further understood that the terms "first," "second," and the like are used to describe various information, but such information should not be limited to these terms. These terms are only used to distinguish one type of information from another and do not denote a particular order or importance. Indeed, the expressions "first", "second", etc. may be used entirely interchangeably. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present disclosure.

Fig. 1 to 16 show a multichannel LAMP detector according to the present embodiment, which is capable of automatically performing nucleic acid amplification detection. Referring to fig. 1, the LAMP detector includes a housing 1, an inner cavity for mounting an internal member is formed in the housing 1, and a chip port 101 for inserting a microfluidic chip 100 is provided in the housing 1. A chip bin 2 is arranged in the shell 1, and the chip bin 2 is provided with a plurality of chip slots 10 for accommodating the microfluidic chip 100. In this embodiment, three chip slots 10 are provided in the chip bin 2, and each chip slot 10 corresponds to one detection channel. In the chip bin 2, a plurality of chip slots 10 are arranged side by side in the front-rear direction, each chip slot 10 has a notch 102 opposite to the chip port 101, specifically, the chip slot 10 is located directly under the chip port 101, the notch of the chip slot 10 faces upward, and the microfluidic chip 100 is inserted into the chip slot 10 from top to bottom.

As shown in fig. 1 and 4, the multi-channel LAMP detector further includes a door 3 movably provided on the inner wall of the housing 1, the door 3 being for closing the chip port 101. Specifically, the door 3 is movably provided on an inner wall of an upper side portion of the housing 1. Further, a first rail 301 extending in the left-right direction is provided on the inner wall of the upper side of the housing 1, and the door 3 is movably provided on the first rail 301 by a slider. The door 3 is driven to open and close by a first driving mechanism. Specifically, the first driving mechanism includes a first motor 302 and a first screw 303 driven to rotate by the first motor 302, and the first screw 303 and the door 3 are connected by threads (for example, by screw nuts). The first screw 303 can be driven to rotate by the first motor 302, and the rotational motion of the first motor 302 is converted into linear motion by the cooperation of the screw and the screw nut, so that the door 3 can be moved left and right. The first guide rails 301 are provided in parallel to each other and are provided on both front and rear sides of the door 3. Detecting opening and closing of the door 3 by a door sensor, the door sensor being electrically connected to a door controller, the door controller being further electrically connected to the first motor 302; when the door sensor is triggered, a detection signal indicating that the door 3 is opened or closed is sent out, and the door controller sends out a control signal for controlling the first motor 302 to rotate forward or backward after receiving the detection signal, so that the door 3 is driven to be closed or opened. The door controller is arranged on a door control PCB 93, and the PCB 93 is arranged on the inner wall of the shell 1. After the microfluidic chip is installed, the door 3 is automatically closed to seal the chip port 101, so that internal components are protected, and the microfluidic chip is attractive.

Referring to fig. 1, 2, 5 and 6, the multi-channel LAMP detector further includes a code scanner 501 for reading a bar code on the microfluidic chip 100. The code scanner 501 is arranged in the shell 1 through the code scanner bracket 502, a scanning window 503 is arranged on the shell 1, and the scanning part of the code scanner 501 is positioned in the scanning window 503 or is opposite to the scanning window 503. The code scanner bracket 502 comprises a base 5021 and a supporting table 5022 which is arranged on the base 5021 and is relatively parallel to the base 5021, and the supporting table 5022 is wedge-shaped. The base 5021 is further provided with a connecting hole 13, and the code scanner bracket 502 is fixedly arranged on the inner wall of the shell 1 through a connecting piece penetrating through the connecting hole 13. The chip port 101 and the code scanner 501 are both located on the upper side of the housing 1 and both face upward.

The multi-channel LAMP detector further comprises a main controller and a display screen 504 (specifically, a touch display screen), wherein the main controller is electrically connected with the code scanner 501 and the display screen 504 respectively. The main controller is disposed on the main PCB board 91. The display 504 has a first display state in which the display 504 has a scan code entry button. After the code-scanning entry button is pressed for the first time, the information of the microfluidic chip 100 read by the code scanner 501 is associated with a first detection channel (for example, the chip slot 10 at the forefront side); after the code scanning input button is pressed for the second time, the information of the microfluidic chip 100 read by the code scanner 501 is associated with a second detection channel (for example, the middle chip slot 10); after the third pressing of the scan code entry button, the information of the microfluidic chip 100 read by the scan code device 501 is associated with a third detection channel (e.g., the rearmost chip slot 10). The display screen 504 is located on the left side portion of the housing 1.

Referring to fig. 7 to 8, the multi-channel LAMP detector further includes a heating assembly for heating the microfluidic chip 100 in the chip slot 10 at a constant temperature. Specifically, the heating assembly is used to heat the reaction chamber of the microfluidic chip 100. Each chip slot 10 corresponds to at least one heating component, and the heating components are attached to the side walls of the chip slots 10. Each heating assembly includes a first heating module 601 and a second heating module 602, the first heating module 601 includes a first insulation cotton press block 6011, a first heating block 6012, a first heating film 6013, a first insulation cotton 6015, and a first heat insulating sheet 6014, and the second heating module 602 includes a second insulation cotton press block 6021, a second heating block 6022, a second heating film 6023, a second insulation cotton 6025, and a second heat insulating sheet 6024. The structures of the first heating module 601 and the second heating module 602 are similar, except that the shapes of the second insulation cotton press block 6021 and the second heating block 6022 of the second heating module 602 are different from the shapes of the first insulation cotton press block 6011 and the first heating block 6012 of the first heating module 601. Here, taking the first heating module 601 as an example, the first heating block 6012 and the first heat insulating sheet 6014 are covered and arranged on the first heating film 6013, the surface of the first heating film 6013 is provided with a glue layer, the first heating film 6013 is covered and arranged on the front surface of the first heat insulation cotton 6015, the back surface of the first heat insulation cotton 6015 is provided with the first heat insulation cotton pressing block 6011, the first heat insulation cotton pressing block 6011 is provided with a connecting hole 13, and the first heating module 601 is arranged on the side wall of the chip slot 10 through a connecting piece passing through the connecting hole 13. The first heating block 6012 is fixedly provided with a first temperature sensor 603, the second heating block 6022 is fixedly provided with a second temperature sensor 604, and the first temperature sensor 603 and the second temperature sensor 604 are respectively and electrically connected with a controller (which may be the above-mentioned main controller). The controller is used for correspondingly controlling the magnitude of the voltage supplied to the first heating film 6013 according to the temperature information detected by the first temperature sensor 603, so as to maintain the heating temperature of the first heating block 6012 constant; similarly, the controller is configured to control the magnitude of the voltage supplied to the second heating film 6023 in accordance with the temperature information detected by the second temperature sensor 604, thereby maintaining the heating temperature of the second heating block 6022 constant.

Fig. 15a and 15b show a partial structure of a single chip slot 10 in a chip bin, in which a microfluidic chip 100 may be inserted into the chip slot 10. As shown in fig. 15a, a hollow area 10a is provided on the side wall of the chip slot 10 to facilitate the cooperation with the heating component; the bottom wall of the chip slot 10 is provided with a hollowed-out area 10b so as to be matched with the optical detection mechanism 8. The first heating block 6012 is matched with a hollowed-out area 10a at the upper part of the side wall of the chip slot 10 in shape, and is embedded into the hollowed-out area 10a, so that the first heating block 6012 can be attached to a microfluidic chip in the chip slot 10; similarly, the second heating block 6022 is matched with a hollowed-out area 10a at the lower part of the side wall of the chip slot 10 in shape, and is embedded into the hollowed-out area 10a, so that the second heating block 6022 can be attached to a microfluidic chip in the chip slot 10. In addition, the part which is not attached to the microfluidic chip is provided with a heat insulating sheet, namely, the high-temperature part of the first heating module 601 is separated from the side wall of the chip slot 10 by the first heat insulating sheet 6014, and the high-temperature part of the second heating module 602 is separated from the side wall of the chip slot 10 by the second heat insulating sheet 6024, so that the problem of premature aging caused by long-time contact of the chip slot 10 with a high-temperature environment is avoided.

Referring to fig. 9 and 10, the multi-channel LAMP detector further includes a mechanical arm mechanism 7, where the mechanical arm mechanism 7 includes a plurality of mechanical arms capable of being engaged with the piston 100a of the microfluidic chip 100 in the chip bin 2, and the mechanical arms are used to drive the piston 100a to move. Each chip slot 10 corresponds to at least one mechanical arm, and each mechanical arm is movably arranged in the shell 1 along the left-right direction and is positioned on the right side of the chip bin 2. The mechanical arm mechanism 7 includes a bracket 701 provided on an inner wall of the housing 1, an upper mounting plate 702 provided on the bracket 701 so as to be movable in a front-rear direction, and a lower mounting plate 703 provided on the bracket 701 so as to be movable in a left-right direction, the upper mounting plate 702 being located above the lower mounting plate 703, a plurality of first mechanical arms 704 being provided on the upper mounting plate 702, a plurality of second mechanical arms 705 being provided on the lower mounting plate 703, each chip slot 10 corresponding to at least one first mechanical arm 704 and at least one second mechanical arm 705, respectively, and the second mechanical arm 705 being located below left of the first mechanical arm 704 when in an initial position.

Referring to fig. 9, 10 and 13, the first and second robot arms 704 and 705 each have an engagement groove 706 into which an end portion of the piston 100a of the microfluidic chip 100 (specifically, the neck portion 100a1 of the piston 100 a) is engaged, and the engagement groove 706 has an upward facing notch 102. The first arm 704 and the second arm 705 are provided at a distance from each other in the front-rear direction and the left-right direction in a plan view, and can be coupled to the two pistons 100a at the same time. Specifically, the second mechanical arm 705 is located at the lower rear of the first mechanical arm 704, i.e., the second mechanical arm 705 is located at one end distance of the rear side of the first mechanical arm 704 to match the positions of the two pistons 100 a.

Referring to fig. 9 and 10, the mechanical arm mechanism 7 further includes a second driving mechanism for driving the upper mounting plate 702 to move left and right, and a third driving mechanism for driving the lower mounting plate 703 to move left and right, the bracket 701 is provided with a second guide 709 and a third guide (not shown) extending in the left and right direction, the upper mounting plate 702 is movably provided on the second guide 709 in the left and right direction, the lower mounting plate 703 is movably provided on the third guide in the left and right direction, the second driving mechanism includes a second motor 7071 and a second screw 7072 driven to rotate by the second motor 7071, the second screw 7072 is connected to the upper mounting plate 702, the third driving mechanism includes a third motor 7081 and a third screw (not shown) driven to rotate by the third motor 7081, and the third screw is connected to the lower mounting plate 703. The robot arm mechanism 7 further includes a first photoelectric switch 711 for detecting whether the first robot arm 704 reaches its initial position, and a second photoelectric switch 712 for detecting whether the second robot arm 705 reaches its initial position. The bracket 701 has a blocking portion 7011 for preventing the upper and lower mounting plates 702 and 703 from moving rightward beyond their set travel. The second motor 7071 and the third motor 7081 are further electrically connected to a mechanical arm controller, and the mechanical arm controller is configured to send control signals to control the operation of the second motor 7071 and the third motor 7081, respectively, and is further electrically connected to the first photoelectric switch 711 and the second photoelectric switch 712, respectively, so as to receive signals sent by the first photoelectric switch 711 and the second photoelectric switch 712. The robotic arm controller is disposed on a robotic arm control PCB board 92. According to the embodiment, the first mechanical arm 704 and the second mechanical arm 705 can be accurately controlled, the three first mechanical arms 704 are controlled by the same driving system, the three second mechanical arms 705 are controlled by the same driving system, pistons of the three micro-fluidic chips 100 can be synchronously moved, the control precision is high, and excessive increase of cost is avoided.

The mechanical arm controller sends a control signal to the second motor 7071, so that the second motor 7071 starts to operate, and the second motor 7071 drives the second screw rod 7072 to rotate, so as to drive the upper mounting plate 702 to perform linear motion. The upper mounting plate 702 is provided with a first mechanical arm 704, and the first mechanical arm 704 moves linearly along with the upper mounting plate 702. The first mechanical arm 704 is provided with a first photoelectric switch sensing bracket 7041, the first photoelectric switch sensing bracket 7041 moves along the second guide rail 709 along with the first mechanical arm 704 until the first photoelectric switch sensing bracket 7041 moves to the first photoelectric switch 711, the first photoelectric switch 711 is triggered to send out a detection signal and transmits the detection signal to the mechanical arm controller, and the mechanical arm controller receives the detection signal and then controls the second motor 7071 to reversely run or stop. When the second motor 7071 is operated, the second screw rod 7072 moves forward or backward along the front-back direction, and the first mechanical arm 704 moves forward or backward along the second guide rail 709, so as to drive the piston 100a at the upper part of the microfluidic chip 100 to move left and right, and further provide positive pressure or negative pressure for the liquid in the microfluidic chip 100, thereby providing power for liquid circulation.

Similarly, the mechanical arm controller sends a control signal to the third motor 7081, so that the third motor 7081 starts to operate, and the third motor 7081 drives the third screw rod to rotate, so as to drive the lower mounting plate 703 to perform linear motion. The lower mounting plate 703 is provided with a second mechanical arm 705, and the second mechanical arm 705 moves linearly along with the lower mounting plate 703. The second mechanical arm 705 is provided with a second photoelectric switch sensing bracket 7051. The second photoelectric switch sensing bracket 7051 moves along the third guide rail along with the second mechanical arm 705 until the second photoelectric switch sensing bracket 7051 moves to the second photoelectric switch 712, the second photoelectric switch 712 is triggered to send out a detection signal, and the mechanical arm controller receives the detection signal and then controls the third motor 7081 to reversely run or stop running. When the third motor 7081 is operated, the third screw rod moves leftwards or rightwards along the left-right direction, and the second mechanical arm 705 moves leftwards or rightwards along the third guide rail, so that the piston 100a at the lower part of the microfluidic chip 100 is driven to move leftwards or rightwards, and positive pressure or negative pressure is further provided for liquid in the microfluidic chip 100, so that power for liquid circulation is provided, or on-off between chambers in the microfluidic chip 100 is switched.

Referring to fig. 11a and 11b, the multi-channel LAMP detector includes an optical detection mechanism 8, where the optical detection mechanism 8 is movably disposed in the housing 1 along a left-right direction and a front-back direction, the optical detection mechanism 8 is located below the chip bin 2 to detect each amplification detection cavity of the microfluidic chip 100 in each chip slot 10 one by one in sequence, where the plurality of microfluidic chips 100 are arranged along the front-back direction, each microfluidic chip 100 has a plurality of amplification detection cavities disposed at intervals along the left-right direction, and each amplification detection cavity is a detection site, as shown in fig. 16. For example, the optical detection mechanism 8 may be moved in the left-right direction, so as to scan a plurality of detection sites of the microfluidic chip 100 in one of the chip slots 10 one by one; the optical detection mechanism 8 moves a distance in the front-rear direction, can move to a position aligned with another microfluidic chip 100, and then moves in the left-right direction to scan a plurality of detection sites of the microfluidic chip 100 one by one.

Further, the multi-channel LAMP detector further includes a base 11 provided on the inner wall of the housing 1, the base 11 having an X-guide rail 809 extending in the left-right direction; the multi-channel LAMP detector further includes a mounting block provided movably in the left-right direction on the X-direction guide 809, the mounting block having a Y-direction guide 810 extending in the front-rear direction; the optical detection mechanism 8 is provided on the Y-direction guide 810 so as to be movable in the front-rear direction. The multi-channel LAMP detector further includes an X-direction driving mechanism for driving the mounting block to move along the X-direction guide 809, a Y-direction driving mechanism for driving the optical detection mechanism 8 to move along the Y-direction guide 810, an X-direction start point detection switch 801 for detecting whether the optical detection mechanism 8 reaches its start point in the left-right direction, an X-direction end point detection switch 802 for detecting whether the optical detection mechanism 8 reaches its end point in the left-right direction, a Y-direction start point detection switch 803 for detecting whether the optical detection mechanism 8 reaches its start point in the front-rear direction, and a Y-direction end point detection switch 804 for detecting whether the optical detection mechanism 8 reaches its end point in the front-rear direction, the X-direction start point detection switch 801 and the X-direction end point detection switch 802 being near both ends of the X-direction guide 810, respectively, the Y-direction start point detection switch 803 and the Y-direction end point detection switch 804 being near both ends 809 of the Y-direction guide 810, respectively. Specifically, in this embodiment, the X-direction driving mechanism includes an X-direction motor 805, where the X-direction motor 805 is disposed on the base 11 and drives the Y-direction guide rail 810 to move left and right through cooperation of a screw and a nut; the Y-direction driving mechanism comprises a Y-direction motor 806, and the Y-direction motor 806 is arranged on the mounting block and drives the optical detection mechanism 8 to move forwards and backwards along the Y-direction guide rail through the cooperation of a screw rod and a nut. The X-direction start point detection switch 801, the X-direction end point detection switch 802, the Y-direction start point detection switch 803, and the Y-direction end point detection switch 804 are photoelectric detection switches, respectively. Referring to fig. 16, an X-direction start point detection switch 801 emits a first detection signal when it is detected that the optical detection mechanism 8 reaches its start point in the left-right direction, an X-direction end point detection switch 802 emits a second detection signal when it is detected that the optical detection mechanism 8 reaches its end point in the left-right direction, a Y-direction start point detection switch 803 emits a third detection signal when it is detected that the optical detection mechanism 8 reaches its start point in the front-rear direction, and a Y-direction end point detection switch 804 emits a fourth detection signal when it is detected that the optical detection mechanism 8 reaches its end point in the front-rear direction.

The optical inspection mechanism 8 has a plurality of inspection positions, and each chip slot 10 has a plurality of inspection sites arranged in the left-right direction, one for each inspection site of each chip slot 10. In this embodiment, as shown in fig. 16, the microfluidic chip in each chip slot 10 corresponds to one detection channel, and has three detection channels C1, C2 and C3, and each chip slot 10 has four detection sites, and has twelve detection sites, respectively; specifically, the detection channel C1 has four detection sites P11, P12, P13, and P14, and the detection channel C2 has four detection sites P21, P22, P23, and P24; the detection channel C3 has four detection sites P31, P32, P33 and P34. Taking the chip slot 10 located at the forefront as an example, the optical detection mechanism 8 is made to run from the origin, and moves according to the coordinates formed by the detection sites, after the optical detection mechanism 8 passes through the four detection sites, the optical detection mechanism 8 is made to move to the chip slot 10 at the rear in the Y direction, and the second chip slot 10 is detected, and so on.

The main controller is respectively and electrically connected with an X-direction starting point detection switch 801, an X-direction end point detection switch 802, a Y-direction starting point detection switch 803 and a Y-direction end point detection switch 804, and controls the X-direction driving mechanism to operate in the forward direction after receiving a first detection signal, controls the X-direction driving mechanism to operate in the reverse direction after receiving a second detection signal, controls the Y-direction driving mechanism to operate in the forward direction after receiving a third detection signal and controls the Y-direction driving mechanism to operate in the reverse direction after receiving a fourth detection signal.

Referring to fig. 3 and 12, a mounting frame 101 is provided in the housing 1, and the mounting frame 101 is located between the display screen 504 and the chip bin 2. The mounting frame 101 is provided with a guide chute 101a, and the edge portion of the main PCB 91 is inserted into the guide chute 101 a. The PCB can be installed in a simple structure and low cost mode, the size is small, the installation is convenient, and especially under the condition that the installation space is relatively narrow, the assembly and the maintenance are relatively convenient.

As shown in fig. 14, the multi-channel LAMP detector further includes a chip cover 4, wherein the chip cover 4 is disposed between the inner wall of the housing 1 surrounding the chip port 101 and the chip bin 2 and between the chip slots 10, and the hollowed-out portions thereof correspond to the notches of the chip slots 10 to allow the microfluidic chip 100 to be inserted into the chip slot 10. The chip cover 4 is used for sealing the gap between the chip bin 2 and the inner wall of the shell 1 and the gap between the chip slots 10, preventing foreign matters from falling in, preventing dust and protecting internal components. The chip cover 4 can also play a role of shielding parts in the detector, so that the detector is attractive. The chip cover 4 adopts a 3D printing technology, has a simple structure and can solve the problem of high manufacturing cost.

The working process of the multi-channel LAMP detector of this embodiment is as follows:

After the micro-fluidic chip 100 is aligned to the code scanner 501 and scanned, the micro-fluidic chip 100 is inserted into the chip slot 10, the door 3 is automatically closed, the first mechanical arm 704 and the second mechanical arm 705 of the mechanical arm mechanism 7 move according to a set time sequence, the piston 100a is driven to move, the cavity is communicated, and positive pressure or negative pressure of fluid flow is provided, so that samples and reagents of the micro-fluidic chip 100 are mixed or the reagents are circulated into a target cavity until entering the reaction cavity to perform amplification reaction; powering up the heating assembly to heat the corresponding chamber of the microfluidic chip 100; after the reaction is completed, the optical detection mechanism 8 is powered on and sequentially moves to each detection site according to a set time sequence, and optical detection is performed on each amplification detection cavity.

The embodiment also provides a control method of the multichannel LAMP detector, which comprises the following steps:

A. scanning codes of the microfluidic chip 100, inputting sample information and the like, associating the sample information with a corresponding detection channel C1, C2 or C3, and installing the microfluidic chip 100;

B. the microfluidic chip 100 is subjected to constant temperature heating:

C. the first mechanical arm 704 and the second mechanical arm 705 are controlled to move, so that reaction systems such as samples, primers and the like of the microfluidic chip 100 are mixed and distributed into each amplification cavity;

D. The optical detection mechanism 8 is controlled to move to detect detection sites one by one.

Referring to fig. 16, the process D specifically includes the steps of:

s101, the optical detection mechanism 8 is at the origin position or is moved to the origin position (i.e. the detection site P11), the X-direction origin detection switch 801 and the Y-direction origin detection switch 803 are triggered to emit a first detection signal and a third detection signal, and after receiving the first detection signal and the third detection signal, the main controller determines that the optical detection mechanism 8 is at the origin, and detects the detection site P11 of the first microfluidic chip 100. The main controller establishes a correspondence between the detection information sent by the optical detection mechanism 8 at this time and the detection site P11 of the first detection channel C1.

S102, the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves rightwards by a set distance d1 and stops to a detection site P12 for detection; specifically, the rotation angle of the X-direction motor 805 is calculated by setting the distance d1 (i.e., the distance between two adjacent detection sites), and the X-direction motor 805 stops after rotating by the set angle. The main controller establishes a correspondence between the detection information sent by the optical detection mechanism 8 at this time and the detection site P12 of the first detection channel C1.

And the optical detection mechanism 8 is controlled to sequentially move to the detection sites P13 and P14 for detection, and the main controller sequentially establishes a corresponding relation between detection information sent by the optical detection mechanism 8 and the detection sites P13 and P14 of the first detection channel C1.

Upon detection of the detection site P14 of the first microfluidic chip 100, the X-direction end point detection switch 802 is triggered to emit a second detection signal.

S103, after receiving the second detection signal and waiting for the set time, the main controller controls the X-direction motor 805 to reversely run so that the optical detection mechanism 8 moves leftwards to return to the original point, and at the moment, the X-direction starting point detection switch 801 is triggered to send out a first detection signal; the set time is at least longer than the time required for the optical detection mechanism 8 to complete the optical detection of one detection site.

S104, after the optical detection mechanism 8 returns to the original point, the main controller receives the first detection signal and the third detection signal, controls the Y-direction motor 806 to move backwards by a set distance d2 and then stops to the detection site P21 of the second microfluidic chip 100 for detection; the main controller establishes a correspondence between the detection information sent by the optical detection mechanism 8 at this time and the detection site P21 of the second detection channel C2. Specifically, the rotation angle of the Y-direction motor 806 is calculated by setting the distance d2 (i.e. the distance between two adjacent microfluidic chips 100), and the Y-direction motor 806 is stopped after rotating by the set angle

S105, the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves rightwards by a set distance d1 and stops to a detection site P22 for detection; the main controller establishes a correspondence between the detection information sent by the optical detection mechanism 8 at this time and the detection site P22 of the second detection channel C2.

And the optical detection mechanism 8 is controlled to sequentially move to the detection sites P23 and P24 for detection, and the main controller sequentially establishes a corresponding relation between detection information sent by the optical detection mechanism 8 and the detection sites P23 and P24 of the second detection channel C2.

Upon detection of the detection site P24 of the second microfluidic chip 100, the X-direction end point detection switch 802 is triggered to emit a second detection signal.

And S106, after receiving the second detection signal and waiting for the set time, the main controller controls the X-direction motor 805 to reversely run so as to enable the optical detection mechanism 8 to move leftwards until the X-direction starting point detection switch 801 is triggered to send out a first detection signal.

S107, after the optical detection mechanism 8 returns to the original point, the main controller receives a first detection signal, and after receiving the first detection signal, the main controller controls the Y-direction motor 806 to move d2 backwards to the detection site P31 of the last microfluidic chip 100 for detection; the main controller establishes a correspondence between the detection information sent by the optical detection mechanism 8 at this time and the detection site P31 of the third detection channel C3.

S108, the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves rightwards by a set distance d1 and stops to the detection site P32 for detection; the main controller establishes a correspondence between the detection information sent by the optical detection mechanism 8 at this time and the detection site P32 of the third detection channel C3.

And the optical detection mechanism 8 is controlled to sequentially move to the detection sites P33 and P34 for detection, and the main controller sequentially establishes a corresponding relation between detection information sent by the optical detection mechanism 8 and the detection sites P33 and P34 of the third detection channel C3.

Upon detection of the detection site P44 of the third microfluidic chip 100, the X-direction end point detection switch 802 is triggered to emit a second detection signal.

S109, after receiving the second detection signal and waiting for the set time, the main controller controls the X-direction motor 805 to reversely run so as to enable the optical detection mechanism 8 to move leftwards until the X-direction starting point detection switch 801 is triggered to send out a first detection signal; meanwhile, in the process of detecting the third detection channel, the Y-direction endpoint detection switch 804 is triggered to send out a fourth detection signal;

s110, after receiving the first detection signal and the fourth detection signal, the main controller controls the Y-direction motor 806 to reversely run, so that the optical detection mechanism 8 moves forwards until the X-direction starting point detection switch 801 and the Y-direction starting point detection switch 803 are triggered to send out the first detection signal and the third detection signal, and the Y-direction motor 806 stops running, so that the optical detection mechanism 8 returns to the original point and waits for the next detection.

The process A specifically comprises the following steps:

s201, switching a display screen 504 to a first display state, receiving a first code scanning input instruction of a user through the display screen 504, reading first bar code information of a first microfluidic chip 100 through a code scanner 501, and associating the first bar code information with a first detection channel C1 after receiving the first code scanning input instruction and the first bar code information by a main controller;

s202, receiving a second code scanning input instruction of a user through a display screen 504, reading second bar code information of a second microfluidic chip 100 through a code scanner 501, and associating the second bar code information with a second detection channel C2 after receiving the second code scanning input instruction and the second bar code information by a main controller;

s203, receiving a third code scanning input instruction of a user through a display screen 504, reading third bar code information of a third microfluidic chip 100 through a code scanner 501, and associating the third bar code information with a third detection channel C3 after receiving the third code scanning input instruction and the second bar code information by a main controller.

The bar code information includes the information of the sample to be tested, such as the number, the name of the corresponding subject, the source, etc.

After the detection is completed, the display screen 504 is switched to the second display state, and the detection results (yin/yang) of the detection sites, the associated numbers, the names of the subjects, and the like are displayed.

The multi-channel LAMP detector provided by the embodiment is characterized in that the chip bin 2 is internally provided with the plurality of chip slots 10, so that a plurality of chips can be detected at the same time, a plurality of microfluidic chips 100 can be processed at one time, the number of samples which can be processed once by running a detection program is greatly increased, and the detection efficiency is higher; the arranged door 3 and the chip cover 4 can prevent dust and foreign matters from entering into the gap between the chip bin 2 and the inner wall, wherein the chip cover 4 can also play a role of shielding elements in the detector, so that the appearance is more attractive; a plurality of detection channels of the LAMP detector can share some components, such as a mechanical arm mechanism, an optical detection mechanism 8 and the like, and compared with a single-channel LAMP detector, the LAMP detector effectively utilizes space, does not obviously increase the whole volume and does not occupy extra space. Under the premise of adding a plurality of detection channels, the volume of the detector is still similar to that of a single-channel detector, the structure is more compact, the occupied space is optimized, the volume is small, the operation is simple, the use is convenient, and the cost is saved.

The above-described embodiments are provided for illustrating the technical concept and features of the present invention, and are intended to be preferred embodiments for those skilled in the art to understand the present invention and implement the same according to the present invention, not to limit the scope of the present invention. All equivalent changes or modifications made according to the principles of the present invention should be construed to be included within the scope of the present invention.

Claims

1. The multichannel LAMP detector comprises a shell, a chip bin and an optical detection mechanism, and is characterized in that the chip bin is provided with a plurality of chip slots for accommodating microfluidic chips, and the chip slots are arranged in parallel along the front-back direction; the optical detection mechanism is movably arranged in the shell along the left-right direction and the front-back direction, and is positioned below the chip bin; each chip slot is provided with a plurality of detection sites which are arranged along the left-right direction, the optical detection mechanism is provided with a plurality of detection positions, and each detection site of each chip slot corresponds to one detection position; the multichannel LAMP detector further comprises:

the controller is used for receiving detection signals of the X-direction starting point detection switch, the X-direction end point detection switch, the Y-direction starting point detection switch and the Y-direction end point detection switch, and can control the X-direction driving mechanism to operate in the forward direction after receiving the first detection signal, can control the X-direction driving mechanism to operate in the reverse direction after receiving the second detection signal, can control the Y-direction driving mechanism to operate in the forward direction after receiving the third detection signal, and can control the Y-direction driving mechanism to operate in the reverse direction after receiving the fourth detection signal;

The multi-channel LAMP detector further comprises a code scanner for reading a bar code on the microfluidic chip and a display screen used as a human-computer interaction interface, wherein the display screen is provided with a first display state, when the display screen is in the first display state, the display screen is provided with a code scanning input button for a user to input a code scanning input instruction, and the controller is further used for establishing a corresponding relation between bar code information read by the code scanner and a corresponding detection channel according to a set sequence after the code scanning input instruction is received, and each detection channel corresponds to one chip slot;

the shell is provided with a chip port for inserting the microfluidic chip, and each chip slot is provided with a notch opposite to the chip port; the multi-channel LAMP detector further comprises heating components for heating the microfluidic chip in the chip slot at constant temperature, and at least one heating component is arranged on the side wall of each chip slot; the multichannel LAMP detector further comprises a mechanical arm mechanism, wherein the mechanical arm mechanism comprises a plurality of mechanical arms which can be connected with a piston of a microfluidic chip in the chip bin, each chip slot corresponds to at least one mechanical arm, and each mechanical arm can be movably arranged in the shell along the left-right direction and is positioned on the right side of the chip bin.

2. The multi-channel LAMP detector of claim 1, wherein,

the controller is used for controlling the X-direction driving mechanism to run forward for a first set distance and then stop for a set time when the first detection signal and the third detection signal are received, and repeating the process for one or more times until the second detection signal is received, and controlling the X-direction driving mechanism to run reversely; controlling the X-direction driving mechanism to stop running until the first detection signal is received again; and/or the number of the groups of groups,

3. The multi-channel LAMP detector of claim 1 or 2, wherein the X-direction drive mechanism comprises an X-direction motor, the Y-direction drive mechanism comprises a Y-direction motor, and the controller is electrically connected with the X-direction motor and the Y-direction motor, respectively; and/or the controller is respectively and electrically connected with the X-direction starting point detection switch, the X-direction end point detection switch, the Y-direction starting point detection switch and the Y-direction end point detection switch.

4. The multi-channel LAMP detector as claimed in claim 3, wherein the multi-channel LAMP detector comprises a base having a plurality of X-direction rails arranged side by side and extending in a left-right direction; the multichannel LAMP detector further comprises a mounting block which is movably arranged on the X-direction guide rail along the left-right direction, wherein a Y-direction guide rail extending along the front-back direction is arranged on the mounting block; the optical detection mechanism is movably arranged on the Y-direction guide rail along the front-back direction; the X-direction motor is arranged on the base and is connected with the mounting block or the Y-direction guide rail through an X-direction screw rod, and the Y-direction motor is arranged on the mounting block and is connected with the optical detection mechanism through a Y-direction screw rod; the X-direction starting point detection switch and the X-direction end point detection switch are respectively close to two end parts of the X-direction guide rail, and the Y-direction starting point detection switch and the Y-direction end point detection switch are respectively close to two end parts of the Y-direction guide rail.

5. The multi-channel LAMP detector of claim 1, wherein the controller is further configured to receive the detection data returned by the optical detection mechanism, and wherein the display screen further has a second display state in which the display screen has a result display area corresponding to each detection site of each detection channel.

6. The multi-channel LAMP detector of claim 1, wherein the mechanical arm mechanism comprises a bracket arranged on the inner wall of the shell, a lower mounting plate capable of being arranged on the bracket along the left-right direction and an upper mounting plate capable of being movably arranged on the bracket along the left-right direction, the upper mounting plate is positioned above the lower mounting plate, a plurality of first mechanical arms are arranged on the upper mounting plate, a plurality of second mechanical arms are arranged on the lower mounting plate, each chip slot corresponds to at least one first mechanical arm and at least one second mechanical arm respectively, and the second mechanical arms are positioned below the left of the first mechanical arms in an initial position.

7. The control method of the multi-channel LAMP detector as claimed in any one of claims 1 to 6, characterized in that the control method comprises the steps of:

s107, after the controller receives the first detection signal, controlling the Y-direction driving mechanism to move backwards to the detection site Pn1 of the last microfluidic chip for detection, wherein n is the number of detection channels;

8. The control method according to claim 7, characterized in that the control method further comprises the following step, before step S101:

in step S101, the controller establishes a correspondence between the detection information sent by the optical detection mechanism and the detection site P11 of the first detection channel;

in step S102, the controller sequentially establishes a corresponding relationship between the detection information sent by the optical detection mechanism and other detection sites of the first detection channel;

in step S105, the controller sequentially establishes a corresponding relationship between the detection information sent by the optical detection mechanism and other detection sites of the second detection channel;

in step S107, the controller establishes a correspondence between the detection information sent by the optical detection mechanism and the detection site Pn1 of the last detection channel;

in step S108, the controller sequentially establishes a corresponding relationship between the detection information sent by the optical detection mechanism and other detection sites of the last detection channel.