CN116183596B - Multichannel LAMP automatic detection system and working method thereof - Google Patents
Multichannel LAMP automatic detection system and working method thereof Download PDFInfo
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- CN116183596B CN116183596B CN202310489283.XA CN202310489283A CN116183596B CN 116183596 B CN116183596 B CN 116183596B CN 202310489283 A CN202310489283 A CN 202310489283A CN 116183596 B CN116183596 B CN 116183596B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N2021/786—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour with auxiliary heating for reaction
Abstract
The invention provides a multichannel LAMP automatic detection system and a working method thereof, wherein the multichannel LAMP automatic detection system comprises: the system comprises a main control module, a plurality of channels and a plurality of LAMP automatic detection devices; each channel is connected with two LAMP automatic detection devices; the main control module is electrically connected with the LAMP automatic detection device through the channel. By adopting a multi-channel connection mode, the LAMP automatic detection device is controlled, the LAMP detection efficiency is improved, meanwhile, each channel can control two LAMP automatic detection devices, and the manufacturing cost of the multi-channel is reduced by adopting a multiplexing technology.
Description
Technical Field
The invention relates to the field of detection, in particular to a multichannel LAMP automatic detection system and a working method thereof.
Background
The LAMP color reaction is used as one of the main methods of biological identification, and at present, the LAMP reaction is followed by visual color identification, so that the result is unreliable, the consistency cannot be ensured, and the subjectivity is high. And automation cannot be realized; after the LAMP reaction, the LAMP reaction is identified by a traditional image sensor, so that automation can be realized.
Because the current automatic LAMP reaction color recognition device is a single channel, batch LAMP color reaction cannot be carried out.
The above problems are currently in need of solution.
Disclosure of Invention
The invention aims to provide a multichannel LAMP automatic detection system and a working method thereof.
In order to solve the above technical problems, the present invention provides a multi-channel LAMP automatic detection system, including:
the system comprises a main control module, a plurality of channels and a plurality of LAMP automatic detection devices;
each channel is connected with two LAMP automatic detection devices;
the main control module is electrically connected with the LAMP automatic detection device through the channel.
Further, the LAMP automation detection device includes:
the system comprises a temperature control module, an LAMP reaction module and a spectrum sampling module;
the temperature control module is suitable for heating the LAMP reaction module;
the spectrum sampling module is suitable for spectrum sampling of the LAMP reaction module;
the temperature control module and the spectrum sampling module are electrically connected with the main control module through corresponding channels.
Further, the main control module sends PWM signals to the corresponding channels to control the temperature control module of the corresponding LAMP automatic detection device to heat, and the temperature control module adopts peak staggering heating.
Further, the off-peak heating, i.e., a group of 2 channels, wherein the PWM signal of one channel is delayed by half a period.
Further, the temperature control module includes:
the device comprises a heating plate, a temperature sensor, a working cavity and a temperature measuring cavity;
the heating plate and the temperature sensor are electrically connected with the main control module;
the working cavity and the temperature measuring cavity are arranged on the heating plate;
the temperature sensor is arranged in the temperature measuring cavity;
the working cavity and the temperature measuring cavity are symmetrically arranged.
Further, the PWM and temperature sensors in the LAMP automatic detection device are respectively and electrically connected with the PWM output interface and the ADC interface corresponding to the main control module;
the temperature control module, the LAMP reaction module and the spectrum sampling module in the LAMP automatic detection device are electrically connected with the corresponding interfaces of the main control module in a multiplexing mode.
Further, the rest control signals are spectrum detection signals, spectrum LED light supplementing signals, flow control photoresistor signals and flow control LED light supplementing signals.
Further, the LAMP reaction module comprises a detection cavity;
the detection cavity is provided with an injection port into which a sample to be detected enters.
Further, the spectrum sampling module includes:
the LED light emitting diode comprises a spectrum sensor, a photoresistor, an LED light emitting tube, a micro-fluidic chip PCB and a micro-fluidic chip;
the spectrum sensor is arranged opposite to the detection cavity and is suitable for sending the collected spectrum signals to the main control module;
the photoresistor is suitable for detecting the light intensity of the detection cavity;
the LED is suitable for supplementing light to the detection cavity;
the micro-fluidic chip PCB and the micro-fluidic chip are suitable for controlling the photoresistor and the LED.
The invention also provides a working method of the multichannel LAMP automatic detection system, which comprises the following steps:
injecting a sample to be detected into a corresponding LAMP automatic detection device;
the main control module receives a detection instruction;
and the main control module controls the corresponding LAMP automatic detection device to carry out LAMP detection through the corresponding channel according to the received detection instruction.
The invention has the beneficial effects that the invention provides a multichannel LAMP automatic detection system and a working method thereof, wherein the multichannel LAMP automatic detection system comprises: the system comprises a main control module, a plurality of channels and a plurality of LAMP automatic detection devices; each channel is connected with two LAMP automatic detection devices; the main control module is electrically connected with the LAMP automatic detection device through the channel. By adopting a multi-channel connection mode, the LAMP automatic detection device is controlled, the LAMP detection efficiency is improved, meanwhile, each channel can control two LAMP automatic detection devices, and the manufacturing cost of the multi-channel is reduced by adopting a multiplexing technology.
Drawings
The invention will be further described with reference to the drawings and examples.
Fig. 1 is a schematic block diagram of a multi-channel LAMP automation detection system provided by an embodiment of the present invention.
Fig. 2 is a schematic structural diagram of an LAMP automatic detection device according to an embodiment of the present invention.
Fig. 3 is a control signal multiplexing circuit diagram according to an embodiment of the present invention.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings. The drawings are simplified schematic representations which merely illustrate the basic structure of the invention and therefore show only the structures which are relevant to the invention.
Example 1
Referring to fig. 1-3, an embodiment of the present invention provides a multi-channel LAMP automation detection system, including: the system comprises a main control module, a plurality of channels and a plurality of LAMP automatic detection devices; each channel is connected with two LAMP automatic detection devices; the main control module is electrically connected with the LAMP automatic detection device through the channel. By adopting a multi-channel connection mode, the LAMP automatic detection device is controlled, the LAMP detection efficiency is improved, meanwhile, each channel can control two LAMP automatic detection devices, and the manufacturing cost of the multi-channel is reduced by adopting a multiplexing technology.
In this embodiment, the LAMP automation detection device includes: the system comprises a temperature control module, an LAMP reaction module and a spectrum sampling module; the temperature control module is suitable for heating the LAMP reaction module; the spectrum sampling module is suitable for spectrum sampling of the LAMP reaction module; the temperature control module and the spectrum sampling module are electrically connected with the main control module through corresponding channels. A schematic structure of the LAMP automatic detection device is shown in FIG. 2.
In this embodiment, the main control module sends a PWM signal to the corresponding channel, and controls the temperature control module of the corresponding LAMP automation detection device to heat, where the temperature control module adopts peak-shifting heating. The off-peak heating, i.e., a group of 2 channels, wherein the PWM signal of one channel is delayed by half a period. Thereby reducing peak current.
In this embodiment, the temperature control module includes: the device comprises a heating plate, a temperature sensor, a working cavity and a temperature measuring cavity; the heating plate and the temperature sensor are electrically connected with the main control module; the working cavity and the temperature measuring cavity are arranged on the heating plate; the temperature sensor is arranged in the temperature measuring cavity; the working cavity and the temperature measuring cavity are symmetrically arranged.
Specifically, the temperature control module adopts a symmetrical structure method, and a temperature measuring cavity is arranged at the parallel position of the working cavity, so that the temperature of the working cavity can be more approximate to the temperature of the working cavity by measuring the temperature of the temperature measuring cavity. And accurate measurement can be achieved through calibration and correction. The main control module controls the heating plate to generate heat by using the PWM through the driving circuit, the temperature sensor is used for feeding back the temperature of the temperature measuring cavity, and the constant temperature control is realized through a PID feedback algorithm. The PID feedback algorithm is prior art and is not explained in relation to this embodiment.
The heating plate may be, but not limited to, a ceramic heating plate. The model of the temperature sensor is PT1000.
In this embodiment, PWM and temperature sensors in the LAMP automatic detection devices are respectively electrically connected with PWM output interfaces and ADC interfaces corresponding to the main control module; the temperature control module, the LAMP reaction module and the spectrum sampling module in the LAMP automatic detection device are electrically connected with the corresponding interfaces of the main control module in a multiplexing mode.
Specifically, in two LAMP automation detection devices of the same channel, part of signals are multiplexed into one interface. The multiplexing circuit diagram is shown in fig. 3.
The rest control signals are spectrum detection signals, spectrum LED light supplementing signals, flow control photoresistor signals and flow control LED light supplementing signals.
In this embodiment, the LAMP reaction module includes a detection chamber; the detection cavity is provided with an injection port into which a sample to be detected enters. Specifically, the sample to be measured is injected into the working cavity through the injection port, then the main control module controls the temperature control module to heat the working cavity to 70 ℃, the temperature is kept for 20 minutes, and the LAMP reaction is completed.
In this embodiment, the spectrum sampling module includes: the LED light emitting diode comprises a spectrum sensor, a photoresistor, an LED light emitting tube, a micro-fluidic chip PCB and a micro-fluidic chip; the spectrum sensor is arranged opposite to the detection cavity and is suitable for sending the collected spectrum signals to the main control module; the photoresistor is suitable for detecting the light intensity of the detection cavity; the LED is suitable for supplementing light to the detection cavity; the micro-fluidic chip PCB and the micro-fluidic chip are suitable for controlling the photoresistor and the LED.
Specifically, after the LAMP reaction is completed, the working cavity presents a specific color; the main control module controls the LED luminous tube to emit a supplementary light source and irradiate the working cavity through the micro-fluidic chip PCB and the micro-fluidic chip, and then obtains a spectrum signal through the spectrum sensor and sends the spectrum signal into the main control module for processing and identification.
Specifically, after the main control module acquires the spectrum signal, the sampling signal is identified and judged through the embedded algorithm model, and a result is given. Meanwhile, a new sample parameter model can be manually added into the algorithm model library to realize model optimization. The specific identification process and model optimization process belong to the prior art, and are not described in the present embodiment.
The master control module adopts the stm32 series singlechip and is matched with the stm32cube xAI software package, so that intelligent identification can be realized under lower hardware environment and software cost, and hardware cost and algorithm complexity are reduced.
The embodiment of the invention also provides a working method of the multichannel LAMP automatic detection system, which comprises the following steps:
injecting a sample to be detected into a corresponding LAMP automatic detection device;
the main control module receives a detection instruction;
and the main control module controls the corresponding LAMP automatic detection device to carry out LAMP detection through the corresponding channel according to the received detection instruction.
The invention provides a multichannel LAMP automatic detection system and a working method thereof, wherein the multichannel LAMP automatic detection system comprises: the system comprises a main control module, a plurality of channels and a plurality of LAMP automatic detection devices; each channel is connected with two LAMP automatic detection devices; the main control module is electrically connected with the LAMP automatic detection device through the channel. By adopting a multi-channel connection mode, the LAMP automatic detection device is controlled, the LAMP detection efficiency is improved, meanwhile, each channel can control two LAMP automatic detection devices, and the manufacturing cost of the multi-channel is reduced by adopting a multiplexing technology.
The components (components not illustrating specific structures) selected in the application are all common standard components or components known to those skilled in the art, and the structures and principles of the components are all known to those skilled in the art through technical manuals or through routine experimental methods. Moreover, the software programs referred to in the present application are all prior art, and the present application does not relate to any improvement of the software programs.
In the description of embodiments of the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the several embodiments provided in this application, it should be understood that the disclosed systems, devices, and methods may be implemented in other manners. The above-described apparatus embodiments are merely illustrative, for example, the division of the units is merely a logical function division, and there may be other manners of division in actual implementation, and for example, multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be through some communication interface, device or unit indirect coupling or communication connection, which may be in electrical, mechanical or other form.
The units described as separate units may or may not be physically separate, and units shown as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional unit in the embodiments of the present invention may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit.
With the above-described preferred embodiments according to the present invention as an illustration, the above-described descriptions can be used by persons skilled in the relevant art to make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but must be determined according to the scope of claims.
Claims (8)
1. A multi-channel LAMP automated detection system, comprising:
the system comprises a main control module, a plurality of channels and a plurality of LAMP automatic detection devices;
each channel is connected with two LAMP automatic detection devices;
the main control module is electrically connected with the LAMP automatic detection device through the channel;
the LAMP automatic detection device comprises:
the system comprises a temperature control module, an LAMP reaction module and a spectrum sampling module;
the temperature control module is suitable for heating the LAMP reaction module;
the spectrum sampling module is suitable for spectrum sampling of the LAMP reaction module;
the temperature control module and the spectrum sampling module are electrically connected with the main control module through corresponding channels;
the main control module sends PWM signals to the corresponding channels to control the temperature control module of the corresponding LAMP automatic detection device to heat, and the temperature control module adopts peak-shifting heating.
2. The multi-channel LAMP automated detection system of claim 1, wherein,
the off-peak heating, i.e., a group of 2 channels, wherein the PWM signal of one channel is delayed by half a period.
3. The multi-channel LAMP automated detection system of claim 1, wherein,
the temperature control module includes:
the device comprises a heating plate, a temperature sensor, a working cavity and a temperature measuring cavity;
the heating plate and the temperature sensor are electrically connected with the main control module;
the working cavity and the temperature measuring cavity are arranged on the heating plate;
the temperature sensor is arranged in the temperature measuring cavity;
the working cavity and the temperature measuring cavity are symmetrically arranged.
4. The multi-channel LAMP automated detection system of claim 1, wherein,
PWM and temperature sensors in the LAMP automatic detection device are respectively and electrically connected with a PWM output interface and an ADC interface corresponding to the main control module;
the temperature control module, the LAMP reaction module and the spectrum sampling module in the LAMP automatic detection device are electrically connected with the corresponding interfaces of the main control module in a multiplexing mode.
5. The multi-channel LAMP automated detection system of claim 4, wherein,
the rest control signals are spectrum detection signals, spectrum LED lamp supplementing signals, flow control photoresistor signals and flow control LED lamp supplementing signals.
6. The multi-channel LAMP automated detection system of claim 1, wherein,
the LAMP reaction module comprises a detection cavity;
the detection cavity is provided with an injection port into which a sample to be detected enters.
7. The multi-channel LAMP automated detection system of claim 6, wherein,
the spectrum sampling module comprises:
the LED light emitting diode comprises a spectrum sensor, a photoresistor, an LED light emitting tube, a micro-fluidic chip PCB and a micro-fluidic chip;
the spectrum sensor is arranged opposite to the detection cavity and is suitable for sending the collected spectrum signals to the main control module;
the photoresistor is suitable for detecting the light intensity of the detection cavity;
the LED is suitable for supplementing light to the detection cavity;
the micro-fluidic chip PCB and the micro-fluidic chip are suitable for controlling the photoresistor and the LED.
8. The method of operating a multi-channel LAMP automation detection system of any one of claims 1-7, comprising:
injecting a sample to be detected into a corresponding LAMP automatic detection device;
the main control module receives a detection instruction;
and the main control module controls the corresponding LAMP automatic detection device to carry out LAMP detection through the corresponding channel according to the received detection instruction.
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