CN213924853U - Multichannel PCR amplification analyzer - Google Patents
Multichannel PCR amplification analyzer Download PDFInfo
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- CN213924853U CN213924853U CN202022585797.XU CN202022585797U CN213924853U CN 213924853 U CN213924853 U CN 213924853U CN 202022585797 U CN202022585797 U CN 202022585797U CN 213924853 U CN213924853 U CN 213924853U
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Abstract
The utility model discloses a multi-channel PCR amplification analyzer, which comprises a casing, an excitation device, a receiving device, a reaction device, a main control circuit board and a touch display screen, wherein the excitation device, the receiving device, the reaction device, the main control circuit board and the touch display screen are arranged on the casing; the excitation device and the receiving device are respectively connected with the reaction device through the excitation optical fiber and the receiving optical fiber, and the main control circuit board is electrically connected with the excitation device, the receiving device, the reaction device and the touch display screen. The utility model has the advantages of compact structure and reliable use.
Description
Technical Field
The utility model relates to the field of biomedical equipment, in particular to a multichannel PCR amplification analyzer.
Background
With the development of molecular biology research and application, the PCR (Polymerase Chain Reaction) technology has gained wide acceptance due to its accuracy and reliability, and the DNA and RNA detection technology developed on this basis is a fundamental stone of molecular biology experiments, and also a tool for human to know the microscopic world and understand itself.
The PCR amplification analyzer in the market at present mainly comprises two functional modules, one is an incubator and the other is a real-time fluorescence reader; the former realizes the heating and heat preservation of a detected sample, and ensures the continuous amplification of nucleic acid at a certain temperature; the latter realizes real-time monitoring of the amount of amplified nucleic acid, and judges the result of the experiment through the change of fluorescence intensity in the sample to be detected.
The existing multi-flux PCR amplification analyzer is characterized in that a plurality of sample test tube positions are arranged in an incubator, a detected sample is placed in a test tube, the test tube is placed in the sample test tube position to be heated, a real-time fluorescence reader is installed at the lower end of the incubator, and the detected sample is monitored in real time through an open hole at the lower end of the sample test tube position. In order to realize real-time monitoring of a plurality of samples, the conventional multi-flux PCR amplification analyzer needs to be provided with a complex electric transmission device, and a real-time fluorescence reader is arranged on the electric transmission device to move so as to detect each detection sample, so that the whole equipment has the defects of large volume, heavy weight, high mechanical failure rate and poor reliability.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an use reliable multichannel PCR amplification analysis appearance.
In order to achieve the above purpose, the solution of the present invention is:
a multi-channel PCR amplification analyzer comprises a casing, an excitation device, a receiving device, a reaction device, a main control circuit board and a touch display screen; the excitation device, the receiving device, the reaction device and the main control circuit board are arranged in the shell, wherein the excitation device and the receiving device are respectively arranged on the left side and the right side of the main control circuit board, and the reaction device is arranged on the rear side of the main control circuit board; the touch display screen is arranged on the top of the shell and is electrically connected with the main control circuit board; the excitation device is provided with at least one excitation module, each excitation module comprises two excitation modules for emitting light rays with different wavelengths, and the excitation modules are electrically connected with the main control circuit board; the reaction device is electrically connected with the main control circuit board, the reaction device is provided with at least one reaction module, each reaction module comprises two reaction modules for heating the reaction cup, and each reaction module is provided with a reaction hole for inserting the reaction cup and a first incident hole, a second incident hole, a first exit hole and a second exit hole which are intersected and communicated with the reaction hole; the reaction modules are matched with the excitation modules in a one-to-one correspondence manner, and a first incident hole and a second incident hole of one of the two reaction modules of each reaction module are respectively matched with the emergent ends of the two excitation modules of the excitation module correspondingly matched with the reaction module through an excitation optical fiber; the first incident hole and the second incident hole of the other reaction module in the two reaction modules of each reaction module are respectively matched with the emergent ends of the two excitation modules of the excitation module correspondingly matched with the reaction module through one excitation optical fiber; the receiving device is provided with at least two receiving modules, each receiving module comprises two receiving modules for receiving light rays with different wavelengths, and the receiving modules are electrically connected with the main control circuit board; each receiving module is correspondingly matched with at least one reaction module, and each reaction module is correspondingly matched with one receiving module; the first emergent hole and the second emergent hole of each reaction module are respectively connected with the incident ends of the two receiving modules of the receiving module correspondingly matched with the reaction module through a receiving optical fiber.
The excitation device is provided with a device mounting seat and a module mounting seat; the device mounting seat is connected with the shell and positioned on the left side of the main control circuit board, and a mounting cavity is formed in the device mounting seat; the module mounting seat is arranged in the mounting cavity and provided with mounting hole groups with the same number as that of the excitation modules, and each mounting hole group comprises two mounting holes which are arranged in a left-right penetrating manner; the excitation modules are correspondingly matched with the mounting hole groups one by one, and the two excitation modules of each excitation module are respectively arranged in two mounting holes of the mounting hole group correspondingly matched with the excitation module; the first end of each excitation optical fiber is inserted into the right opening of the installation pore channel provided with the excitation module matched with the excitation optical fiber, the second end of each excitation optical fiber matched with each first incidence hole of the reaction module is respectively inserted into each first incidence hole, and the second end of each excitation optical fiber matched with each second incidence hole of the reaction module is respectively inserted into each second incidence hole.
The excitation module comprises an LED lamp, an excitation lens and an excitation optical filter which are sequentially arranged in the mounting hole from left to right, and the LED lamp is electrically connected with the main control circuit board.
The receiving device is provided with a device placing seat and a module placing seat; the device placing seat is connected with the shell and positioned on the right side of the main control circuit board, and is provided with a placing cavity; the module placing seat is arranged in the placing cavity and provided with placing hole groups with the same number as that of the receiving modules, and each placing hole group comprises two placing hole channels which are arranged in a left-right penetrating manner; the receiving modules are correspondingly matched with the arrangement hole groups one by one, and two receiving modules of each receiving module are respectively arranged in two arrangement holes of the arrangement hole group correspondingly matched with the receiving module; the first end of each receiving optical fiber is inserted into the left opening of the mounting hole channel provided with the receiving module matched with the receiving optical fiber, the second end of each receiving optical fiber matched with each first emergent hole of the reaction module is respectively inserted into each first emergent hole, and the second end of each receiving optical fiber matched with each second emergent hole of the reaction module is respectively inserted into each second emergent hole.
The receiving module comprises a receiving lens, a receiving optical filter and a photoelectric sensor which are sequentially arranged in the arranging pore channel from left to right, and the photoelectric sensor is electrically connected with the main control circuit board.
The reaction module comprises an upper heating block, a heat insulation sheet and a lower heating block which are sequentially arranged from top to bottom; the upper heating block, the heat insulation sheet and the lower heating block of the reaction module are respectively provided with an upper hole, a middle hole and a lower hole, and the upper hole of the upper heating block, the middle hole of the heat insulation sheet and the lower hole of the lower heating block of the reaction module are correspondingly connected to form the reaction hole; the side wall of the upper heating block is provided with a first incident hole, a second incident hole, a first exit hole and a second exit hole which are communicated with the upper hole; the reaction device is also provided with a reaction mounting seat, a low-temperature heater and a high-temperature heater; the reaction mounting seat is connected with the shell and is positioned behind the main control circuit board; the low-temperature heater, the high-temperature heater and the reaction modules of the reaction modules are all arranged on the reaction mounting seat; the low-temperature heater is contacted with the upper heating block of each reaction module, the high-temperature heater is contacted with the lower heating block of each reaction module, and the main control circuit board is electrically connected with the low-temperature heater and the high-temperature heater.
The upper heating block of each reaction module is integrally formed on an upper heating plate, the lower heating block of each reaction module is integrally formed on a lower heating plate, the heat insulation sheet of each reaction module is integrally formed on a heat insulation plate, and the upper heating plate, the lower heating plate and the heat insulation plate are fixedly connected with the reaction mounting seat.
The reaction device is also provided with a heat dissipation plate, and the heat dissipation plate is arranged on the rear side surface of the reaction mounting seat; the heat dissipation plate is provided with a heat dissipation plate fan electrically connected with the main control circuit board, the heat dissipation plate fan is arranged on the rear side face of the heat dissipation plate, and fan holes are formed in the rear portion of the machine shell corresponding to the heat dissipation plate fan.
The multichannel PCR amplification analyzer further comprises a light shielding device, wherein the light shielding device comprises light shielding seats and light shielding covers, and the number of the light shielding seats is the same as that of the reaction modules; the shading seat is fixed on the top of the shell and provided with grooves which are the same as the reaction modules in number and have upward openings, and the grooves are arranged in one-to-one correspondence with the reaction modules; two through holes communicated with the inner cavity of the shell are formed in each groove, and the two through holes in each groove are respectively arranged opposite to two reaction module reaction holes of the reaction module correspondingly arranged in the groove; each shading cover is hinged with the shading seat and covers the opening of each groove.
The multichannel PCR amplification analyzer also comprises a cooling fan; the heat dissipation fan is arranged in the machine shell, and heat dissipation holes corresponding to the heat dissipation fan are formed in the bottom of the machine shell; the main control circuit board is electrically connected with the heat dissipation fan, a through hole is formed in the middle of the main control circuit board, and the heat dissipation fan is sleeved in the through hole.
After the scheme is adopted, the utility model has the characteristics of it is following:
1. compared with the prior art, the utility model, need not set up complicated electronic transmission device for the good reliability of whole equipment, light in weight.
2. The utility model discloses the multiplexing of receiving module is realized to the control mode of accessible timesharing multiplexing to reduce the quantity of receiving module, realize that the structure is simplified.
3. The utility model discloses an excitation device and receiving arrangement are located the left and right sides of main control circuit board respectively, and reaction unit is located the rear side of main control circuit board, and main control circuit board top can provide sufficient space like this and make things convenient for people to lay and arouse optic fibre and receive optic fibre for arouse the top of laying in main control circuit board that optic fibre and receiving optic fibre can be convenient.
Drawings
Fig. 1 is a schematic structural diagram 1 of the present invention;
fig. 2 is a schematic structural diagram 2 of the present invention;
fig. 3 is a schematic view of a partial structure of the present invention 1;
fig. 4 is a schematic view of a partial structure of the present invention 2;
fig. 5 is a schematic view of a partial structure of the present invention 3;
fig. 6 is an exploded view of the excitation device of the present invention;
fig. 7 is a cross-sectional view of the excitation device of the present invention 1;
fig. 8 is a cross-sectional view 2 of the excitation device of the present invention;
fig. 9 is an exploded view of a receiver of the present invention;
fig. 10 is a cross-sectional view of a receiving device of the present invention 1;
fig. 11 is a cross-sectional view 2 of a receiver of the present invention;
fig. 12 is an exploded view of a reaction apparatus of the present invention;
fig. 13 is an exploded view of the shade device of the present invention;
fig. 14 is a partial cross-sectional view of the present invention 1;
FIG. 15 is an enlarged view at A of FIG. 14;
fig. 16 is a partial cross-sectional view of the present invention, fig. 2;
FIG. 17 is an enlarged view at B of FIG. 16;
description of reference numerals:
a case 1, an upper case 101, a lower case 102, heat radiating holes 11, fan holes 12,
the excitation device (2) is arranged in the shell,
an excitation module 21, an excitation module 211, an LED lamp 2111, an excitation lens 2112, an excitation filter 2113,
the assembly mounting base 22, the mounting cavity 221,
a module mounting seat 23, a mounting hole group 231, a mounting hole 2311,
the circuit board 24 is activated and,
the reception means 3 are arranged to receive the data,
a receiving module 31, a receiving module 311, a receiving filter 3111, a receiving lens 3112, a photoelectric sensor 3113,
the device placement seat 32, the placement cavity 321,
a module seat 33, a set of mounting holes 331, a set of mounting holes 3311,
the circuit board 34 is received and the circuit board is,
a reaction device 4, a reaction module 41, a reaction module 411, an upper heating block 4101, a heat shield 4102, a lower heating block 4103, an upper hole 4104, a middle hole 4105, a lower hole 4106, a reaction hole 4111, a first incident hole 4112, a second incident hole 4113, a first exit hole 4114, a second exit hole 4115,
the reaction-mounting base 42 is mounted on the reaction-mounting base,
the low-temperature heating means (43) is provided,
the high-temperature heater 44 is provided with a high-temperature heater,
the heat radiating plate 45, the heat radiating plate fan 451,
the main control circuit board 5, the through hole 51,
the touch-sensitive display screen 6 is touched,
the light shielding device 7, the light shielding seat 71, the groove 711, the through hole 712, the light shielding cover 72,
the heat-radiating fan (8) is provided with a fan,
the power supply board (9) is provided with a power supply board,
an excitation fiber S and a receiving fiber R.
Detailed Description
In order to further explain the technical solution of the present invention, the present invention is explained in detail by the following embodiments.
As shown in fig. 1 to 17, the utility model discloses a multi-channel PCR amplification analyzer, which comprises a casing 1, an excitation device 2, a receiving device 3, a reaction device 4, a main control circuit board 5 and a touch display screen 6; the excitation device 2, the receiving device 3, the reaction device 4 and the main control circuit board 5 are arranged in the machine shell 1, and the machine shell 1 can be formed by splicing an upper shell 101 and a lower shell 102; the excitation device 2 and the receiving device 3 are respectively positioned at the left side and the right side of the main control circuit board 5, and the reaction device 4 is positioned at the rear side of the main control circuit board 5; the touch display screen 6 is installed on the top of the casing 1, the touch display screen 6 is electrically connected with the main control circuit board 5, and the touch display screen 6 is used for controlling the main control circuit board 5 and displaying a detection result. The excitation device 2 is provided with at least one excitation module 21, each excitation module 21 comprises two excitation modules 211 used for emitting light rays with different wavelengths, the excitation modules 211 are electrically connected with the main control circuit board 5, and the main control circuit board 5 controls whether the excitation modules 211 emit the light rays. The receiving device 3 is provided with at least two receiving modules 31, each receiving module 31 includes two receiving modules 311 for receiving light with different wavelengths, and the receiving modules 311 are electrically connected with the main control circuit board 5. The reaction device 4 is electrically connected with the main control circuit board 5, the reaction device 4 is provided with at least one reaction module 41, each reaction module 41 comprises two reaction modules 411 for heating a reaction cup, the main control circuit board 5 controls the heating temperature of the reaction modules 411, and each reaction module 411 is provided with a reaction hole 4111 for inserting the reaction cup, and a first incident hole 4112, a second incident hole 4113, a first emergent hole 4114 and a second emergent hole 4115 which are communicated with the reaction hole 4111 in an intersecting manner. The reaction modules 41 are correspondingly matched with the excitation modules 21 one by one, a first incident hole 4112 and a second incident hole 4113 of one reaction module 411 of two reaction modules 411 of each reaction module 41 are respectively matched with the emergent ends of two excitation modules 211 of the excitation module 21 correspondingly matched with the reaction module 41 through one excitation optical fiber S, and a first incident hole 4112 and a second incident hole 4113 of the other reaction module 411 of two reaction modules 41 are also respectively matched with the emergent ends of two excitation modules 211 of the excitation module 21 correspondingly matched with the reaction module 41 through one excitation optical fiber S, so that light rays with different wavelengths emitted by the two excitation modules 211 can enter the reaction holes 4111 of the two reaction modules 411, and further the sample inserted in the reaction hole 4111 is irradiated with light rays with two wavelengths, causing the sample in the reaction cup inserted in each reaction well 4111 to excite fluorescence of two different wavelengths; each reaction module 411 is correspondingly matched with one receiving module 31, the first exit hole 4114 and the second exit hole 4115 of each reaction module 411 are respectively connected with the incident ends of the two receiving modules 311 of the receiving module 31 correspondingly matched with the reaction module 411 through one receiving optical fiber R, so that the two types of fluorescence with different wavelengths excited by the sample in the reaction cup inserted in each reaction hole 411 are respectively received by the two receiving modules 311 of one receiving module 31 and converted into corresponding electric signals to be output, the output electric signals are received and analyzed by the main control circuit board 5, and the analysis result is displayed through the touch display screen 6.
In the present invention, each receiving module 31 is correspondingly matched with at least one reaction module 411, so that the present invention realizes the multiplexing of the receiving module 31 by time-sharing multiplexing; specifically, under the condition that is provided with two and more than two excitation module 21 and reaction module 41, every receiving module 31 corresponds the cooperation with two reaction module 411, like this the utility model discloses a control different excitation module 21 and carry out work at the different times to make every receiving module 31 receive the fluorescence that the sample in the different reaction cups arouses in the different times, realize receiving module 31's multiplexing.
As shown in fig. 6 to 8, the excitation device 2 is provided with a device mounting seat 22 and a module mounting seat 23; the device mounting seat 22 is connected with the machine shell 1 and is positioned at the left of the main control circuit board 5, the device mounting seat 22 is provided with a mounting cavity 221, and the device mounting seat 22 can be formed by splicing a plurality of device mounting seat components; the module mounting seat 23 is arranged in the mounting cavity 221, the module mounting seat 23 is provided with mounting hole groups 231 with the same number as that of the excitation modules 21, and each mounting hole group 231 comprises two mounting holes 2311 which penetrate through the left and right; the module mount 23 may be formed by splicing a plurality of module mount members so as to mount the excitation module 21; the excitation modules 21 are correspondingly matched with the installation hole groups 231 one by one, two excitation modules 211 of each excitation module 21 are respectively installed in two installation holes 2311 of the installation hole group 231 correspondingly matched with the excitation module 21, each excitation module 211 comprises an LED lamp 2111, an excitation lens 2112 and an excitation filter 2113 which are sequentially installed in the installation holes 231 from left to right, and the LED lamp 2111 is electrically connected with the main control circuit board 5 so that the main control circuit board 5 controls whether the LED lamp 2111 emits light or not. The first end of each excitation fiber S is inserted into the right opening of the installation hole 2311 of the excitation module 211 matched with the excitation fiber S, the second end of each excitation fiber S matched with each first incident hole 4112 of the reaction module 41 is inserted into each first incident hole 4112, and the second end of each excitation fiber S matched with each second incident hole 4113 of the reaction module 41 is inserted into each second incident hole 4113 of the second incident hole.
As shown in fig. 6 to 8, the excitation device 2 is further provided with an excitation circuit board 24 electrically connected to the main control circuit board 5, the excitation circuit board 24 is installed in the installation cavity 221 and located on the left side of the module installation seat 23, the excitation circuit board 24 covers the left opening of each installation hole 2311 of the module installation seat 23 to prevent stray light from entering the installation hole 2311, the LED lamps 2111 of each excitation module 211 are electrically connected to the excitation circuit board 24, and the LED lamps of each excitation module 211 are connected to the excitation circuit board 24 in a welding manner.
As shown in fig. 9 to 11, the receiving device 3 is provided with a device seat 32 and a module seat 33; the device placing seat 32 is connected with the machine shell 1 and is positioned at the right side of the main control circuit board 5, the device placing seat 32 is provided with a placing cavity 321, and the device placing seat 32 can be formed by splicing a plurality of device placing seat components; the module placing seat 33 is arranged in the placing cavity 321, the module placing seat 33 is provided with a placing hole group 331 with the same number as that of the receiving modules 31, and the placing hole group 331 comprises two placing hole channels 3311 which are arranged in a left-right penetrating manner; the module seat 33 may be formed by splicing a plurality of module seat members so as to mount the receiving module 31; the receiving modules 31 are correspondingly matched with the arrangement hole groups 331 one by one, two receiving modules 311 of each receiving module 31 are respectively installed in two arrangement hole channels 3111 of the arrangement hole group 311 correspondingly matched with the receiving module 31, each receiving module 311 comprises a receiving lens 3112, a receiving optical filter 3111 and a photoelectric sensor 3113 which are sequentially installed in the arrangement hole channels 3111 from left to right, the photoelectric sensor 3113 is electrically connected with the main control circuit board 5, the photoelectric sensor 3113 is used for converting fluorescence into an electric signal, and the photoelectric sensor 3113 can adopt a photodiode; a first end of each receiving optical fiber R is inserted into the left opening of the mounting hole 3311 on which the receiving module 311 is mounted in cooperation with the receiving optical fiber R, a second end of each receiving optical fiber R in cooperation with each first exit hole 4114 of the reaction module 41 is inserted into each first exit hole 4114, and a second end of each receiving optical fiber R in cooperation with each second exit hole 4115 of the reaction module 41 is inserted into each second exit hole 4115.
As shown in fig. 9 to 11, the receiving apparatus 3 further includes a receiving circuit board 34 electrically connected to the main control circuit board 5, the receiving circuit board 34 is installed in the installation cavity 321 and located at the right side of the module installation base 33, the receiving circuit board 34 covers the right openings of the installation holes 3311 of the module installation base 33 to prevent stray light from entering the installation holes 3311, the photoelectric sensor 3113 of each receiving module 311 is electrically connected to the receiving circuit board 34, and the photoelectric sensor 3113 of each receiving module 311 can be connected to the receiving circuit board 34 by soldering.
As shown in fig. 12 and 14 to 17, the reaction apparatus 4 is provided with a reaction mounting seat 42, a low temperature heater 43, and a high temperature heater 44; the reaction mounting seat 42 is connected with the machine shell 1 and is positioned behind the main control circuit board 5; the low-temperature heater 43, the high-temperature heater 44 and the reaction modules 411 of the reaction modules 41 are all arranged on the reaction mounting seat 42; the low temperature heater 43 and the high temperature heater 44 are electrically connected to the main control circuit board 5, the low temperature heater 43 and the high temperature heater 44 heat the reaction modules 411 of the reaction modules 41, and the main control circuit board 5 controls the heating temperatures of the low temperature heater 43 and the high temperature heater 44. The reaction module 411 comprises an upper heating block 4101, a heat insulation sheet 4102 and a lower heating block 4103 which are sequentially arranged from top to bottom, wherein the heat insulation sheet 4102 is used for isolating heat conduction between the upper heating block 4101 and the lower heating block 4103; the upper heating block 4101, the heat shield 4102 and the lower heating block 4103 of the reaction module 411 are respectively provided with an upper hole 4104, a middle hole 4105 and a lower hole 4106, and the upper hole 4104 of the upper heating block 4101, the middle hole 4105 of the heat shield 4102 and the lower hole 4106 of the lower heating block 4103 of the reaction module are correspondingly connected to form the reaction hole 4111; the side wall of the upper heating block 4101 is provided with a first incident hole 4112, a second incident hole 4113, a first exit hole 4114 and a second exit hole 4115 which are communicated with the upper hole 4104, the low temperature heater 43 contacts with the upper heating block 4101 of each reaction module 411 to heat the upper heating block 4101, the high temperature heater 44 contacts with the lower heating block 4103 of each reaction module 411 to heat the lower heating block 4103, the high temperature heater 44 heats the lower heating block 4103 at a temperature higher than that of the low temperature heater 43 for the upper heating block 4101, and further the temperature of the upper heating block 4101 is lower than that of the lower heating block 4103, so that the reaction cup inserted into the reaction hole 4111 is heated in a segmented manner, and further the sample of the reaction cup realizes the convective polymerase chain reaction.
As shown in fig. 12, the upper heating block 4101 of each reaction module 411 is integrally formed on an upper heating plate, the lower heating block 4103 of each reaction module 411 is integrally formed on a lower heating plate, the heat insulating sheet 4102 of each reaction module 411 is integrally formed on a heat insulating plate, and the upper heating plate, the lower heating plate and the heat insulating plate are fixedly connected with the reaction mounting seat 42 by screw fastening, so that each reaction module 411 can be quickly mounted on the reaction mounting seat 42, and the low temperature heater 43 and the high temperature heater 44 can also be fixedly connected with the reaction mounting seat 42 by screw fastening.
As shown in fig. 2 and 5, the reaction apparatus 4 is further provided with a heat dissipation plate 45, and the heat dissipation plate 45 is mounted on the rear side surface of the reaction mounting base 42 through a bolt; the heat radiation plate 45 is provided with a heat radiation plate fan 451 electrically connected with the main control circuit board 5, the heat radiation plate fan 451 is arranged on the rear side surface of the heat radiation plate 45, and the rear part of the machine shell 1 is provided with a fan hole 12 corresponding to the heat radiation plate fan 451; wherein the heat dissipation plate 45 can conduct heat of the reaction mounting seat 25; the main control circuit board 5 controls whether the heat dissipation plate fan 451 operates, and when the heat dissipation plate fan 451 operates, the air current can flow through the heat dissipation plate 45 to reduce the temperature of the heat dissipation plate 45, thereby reducing the temperature of the reaction mounting base 42 and preventing the reaction mounting base 42 from overheating.
In the present invention, the excitation fiber S and the receiving fiber R can both adopt fibers with FC interfaces at both ends, and the FC interfaces at both ends of the excitation fiber S are screwed with the device mounting seat 22 and the reaction mounting seat 42 respectively to fix the excitation fiber S firmly; similarly, the FC ports at the two ends of the receiving fiber R are screwed with the device seat 32 and the reaction seat 42, respectively, so that the receiving fiber R is fixed stably. In addition, the excitation device 2 and the receiving device 3 are respectively arranged at the left side and the right side of the main control circuit board 5, and the reaction device 4 is arranged at the rear side of the main control circuit board 5, so that enough space can be provided above the main control circuit board 5 to facilitate people to arrange the excitation optical fibers S and the receiving optical fibers R, and the excitation optical fibers S and the receiving optical fibers R can be conveniently arranged above the main control circuit board 5.
As shown in fig. 1 and fig. 13 to 17, the present invention further includes a light shielding device 7, wherein the light shielding device 7 includes light shielding bases 71 and light shielding covers 72, the number of which is the same as that of the reaction modules 41; the shading seat 71 is fixed on the top of the machine shell 1, the shading seat 71 is provided with grooves 711 with the same number as the reaction modules 41 and with upward openings, and the grooves 711 and the reaction modules 41 are arranged in a one-to-one correspondence manner; two through holes 712 communicated with the inner cavity of the machine shell 1 are arranged in each groove 711, and the two through holes 712 in each groove 711 are respectively arranged opposite to the two reaction module reaction holes 4111 of the reaction module 71 correspondingly arranged with the groove 711; each light shielding cover 72 is hinged with the light shielding seat 71, and each light shielding cover 72 covers the opening of each groove 711 respectively; when the cuvette is inserted into the through hole 712 and the reaction hole 4111, the light shielding cover 72 covers the opening of the groove 711 to prevent external stray light from entering the cuvette and interfering with the detection.
As shown in fig. 2 and 4, the present invention further includes a heat dissipation fan 8; the heat dissipation fan 8 is arranged in the machine shell 1, and heat dissipation holes 11 are formed in the bottom of the machine shell 1 corresponding to the heat dissipation fan 8; the main control circuit board 5 is electrically connected with the heat dissipation fan 8 to control whether the heat dissipation fan 8 works or not, a through hole 51 is formed in the middle of the main control circuit board 5, the heat dissipation fan 8 is sleeved in the through hole 51, and the heat dissipation fan 8 is used for discharging heat inside the casing 1 out of the casing 1.
As shown in the accompanying drawing 5, the utility model discloses still including the power strip 9 that is used for the power supply, power strip 9 is installed in reaction unit 4's rear side, and power strip 9 is connected and is supplied power for main control circuit board 5 with main control circuit board 5 electricity.
The above embodiments and drawings are not intended to limit the form and style of the present invention, and any suitable changes or modifications made by those skilled in the art should not be construed as departing from the scope of the present invention.
Claims (10)
1. A multichannel PCR amplification analyzer is characterized in that: the device comprises a shell, an excitation device, a receiving device, a reaction device, a main control circuit board and a touch display screen;
the excitation device, the receiving device, the reaction device and the main control circuit board are arranged in the shell, wherein the excitation device and the receiving device are respectively arranged on the left side and the right side of the main control circuit board, and the reaction device is arranged on the rear side of the main control circuit board; the touch display screen is arranged on the top of the shell and is electrically connected with the main control circuit board;
the excitation device is provided with at least one excitation module, each excitation module comprises two excitation modules for emitting light rays with different wavelengths, and the excitation modules are electrically connected with the main control circuit board;
the reaction device is electrically connected with the main control circuit board, the reaction device is provided with at least one reaction module, each reaction module comprises two reaction modules for heating the reaction cup, and each reaction module is provided with a reaction hole for inserting the reaction cup and a first incident hole, a second incident hole, a first exit hole and a second exit hole which are intersected and communicated with the reaction hole; the reaction modules are matched with the excitation modules in a one-to-one correspondence manner, and a first incident hole and a second incident hole of one of the two reaction modules of each reaction module are respectively matched with the emergent ends of the two excitation modules of the excitation module correspondingly matched with the reaction module through an excitation optical fiber; the first incident hole and the second incident hole of the other reaction module in the two reaction modules of each reaction module are respectively matched with the emergent ends of the two excitation modules of the excitation module correspondingly matched with the reaction module through one excitation optical fiber;
the receiving device is provided with at least two receiving modules, each receiving module comprises two receiving modules for receiving light rays with different wavelengths, and the receiving modules are electrically connected with the main control circuit board; each receiving module is correspondingly matched with at least one reaction module, and each reaction module is correspondingly matched with one receiving module; the first emergent hole and the second emergent hole of each reaction module are respectively connected with the incident ends of the two receiving modules of the receiving module correspondingly matched with the reaction module through a receiving optical fiber.
2. The multi-channel PCR amplification analyzer of claim 1, wherein: the excitation device is provided with a device mounting seat and a module mounting seat; the device mounting seat is connected with the shell and positioned on the left side of the main control circuit board, and a mounting cavity is formed in the device mounting seat; the module mounting seat is arranged in the mounting cavity and provided with mounting hole groups with the same number as that of the excitation modules, and each mounting hole group comprises two mounting holes which are arranged in a left-right penetrating manner;
the excitation modules are correspondingly matched with the mounting hole groups one by one, and the two excitation modules of each excitation module are respectively arranged in two mounting holes of the mounting hole group correspondingly matched with the excitation module;
the first end of each excitation optical fiber is inserted into the right opening of the installation pore channel provided with the excitation module matched with the excitation optical fiber, the second end of each excitation optical fiber matched with each first incidence hole of the reaction module is respectively inserted into each first incidence hole, and the second end of each excitation optical fiber matched with each second incidence hole of the reaction module is respectively inserted into each second incidence hole.
3. The multi-channel PCR amplification analyzer of claim 2, wherein: the excitation module comprises an LED lamp, an excitation lens and an excitation optical filter which are sequentially arranged in the mounting hole from left to right, and the LED lamp is electrically connected with the main control circuit board.
4. The multi-channel PCR amplification analyzer of claim 1, wherein: the receiving device is provided with a device placing seat and a module placing seat; the device placing seat is connected with the shell and positioned on the right side of the main control circuit board, and is provided with a placing cavity; the module placing seat is arranged in the placing cavity and provided with placing hole groups with the same number as that of the receiving modules, and each placing hole group comprises two placing hole channels which are arranged in a left-right penetrating manner; the receiving modules are correspondingly matched with the arrangement hole groups one by one, and two receiving modules of each receiving module are respectively arranged in two arrangement holes of the arrangement hole group correspondingly matched with the receiving module;
the first end of each receiving optical fiber is inserted into the left opening of the mounting hole channel provided with the receiving module matched with the receiving optical fiber, the second end of each receiving optical fiber matched with each first emergent hole of the reaction module is respectively inserted into each first emergent hole, and the second end of each receiving optical fiber matched with each second emergent hole of the reaction module is respectively inserted into each second emergent hole.
5. The multi-channel PCR amplification analyzer of claim 4, wherein: the receiving module comprises a receiving lens, a receiving optical filter and a photoelectric sensor which are sequentially arranged in the arranging pore channel from left to right, and the photoelectric sensor is electrically connected with the main control circuit board.
6. The multi-channel PCR amplification analyzer of claim 1, wherein: the reaction module comprises an upper heating block, a heat insulation sheet and a lower heating block which are sequentially arranged from top to bottom; the upper heating block, the heat insulation sheet and the lower heating block of the reaction module are respectively provided with an upper hole, a middle hole and a lower hole, and the upper hole of the upper heating block, the middle hole of the heat insulation sheet and the lower hole of the lower heating block of the reaction module are correspondingly connected to form the reaction hole; the side wall of the upper heating block is provided with a first incident hole, a second incident hole, a first exit hole and a second exit hole which are communicated with the upper hole;
the reaction device is also provided with a reaction mounting seat, a low-temperature heater and a high-temperature heater; the reaction mounting seat is connected with the shell and is positioned behind the main control circuit board; the low-temperature heater, the high-temperature heater and the reaction modules of the reaction modules are all arranged on the reaction mounting seat; the low-temperature heater is contacted with the upper heating block of each reaction module, the high-temperature heater is contacted with the lower heating block of each reaction module, and the main control circuit board is electrically connected with the low-temperature heater and the high-temperature heater.
7. The multi-channel PCR amplification analyzer of claim 6, wherein: the upper heating block of each reaction module is integrally formed on an upper heating plate, the lower heating block of each reaction module is integrally formed on a lower heating plate, the heat insulation sheet of each reaction module is integrally formed on a heat insulation plate, and the upper heating plate, the lower heating plate and the heat insulation plate are fixedly connected with the reaction mounting seat.
8. The multi-channel PCR amplification analyzer of claim 6, wherein: the reaction device is also provided with a heat dissipation plate, and the heat dissipation plate is arranged on the rear side surface of the reaction mounting seat; the heat dissipation plate is provided with a heat dissipation plate fan electrically connected with the main control circuit board, the heat dissipation plate fan is arranged on the rear side face of the heat dissipation plate, and fan holes are formed in the rear portion of the machine shell corresponding to the heat dissipation plate fan.
9. The multi-channel PCR amplification analyzer of claim 1, wherein: the device also comprises a shading device, wherein the shading device comprises shading seats and shading covers the number of which is the same as that of the reaction modules;
the shading seat is fixed on the top of the shell and provided with grooves which are the same as the reaction modules in number and have upward openings, and the grooves are arranged in one-to-one correspondence with the reaction modules; two through holes communicated with the inner cavity of the shell are formed in each groove, and the two through holes in each groove are respectively arranged opposite to two reaction module reaction holes of the reaction module correspondingly arranged in the groove; each shading cover is hinged with the shading seat and covers the opening of each groove.
10. The multi-channel PCR amplification analyzer of claim 1, wherein: the heat dissipation fan is also included; the heat dissipation fan is arranged in the machine shell, and heat dissipation holes corresponding to the heat dissipation fan are formed in the bottom of the machine shell; the main control circuit board is electrically connected with the heat dissipation fan, a through hole is formed in the middle of the main control circuit board, and the heat dissipation fan is sleeved in the through hole.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022585797.XU CN213924853U (en) | 2020-11-10 | 2020-11-10 | Multichannel PCR amplification analyzer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022585797.XU CN213924853U (en) | 2020-11-10 | 2020-11-10 | Multichannel PCR amplification analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115216401A (en) * | 2022-09-20 | 2022-10-21 | 山东博旭基因科技有限公司 | Real-time fluorescence quantitative PCR instrument |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115216401A (en) * | 2022-09-20 | 2022-10-21 | 山东博旭基因科技有限公司 | Real-time fluorescence quantitative PCR instrument |
| CN115216401B (en) * | 2022-09-20 | 2022-12-09 | 山东博旭基因科技有限公司 | Real-time fluorescence quantitative PCR instrument |
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