CN112029655A - Real-time fluorescence nucleic acid detector with rapid detection function - Google Patents
Real-time fluorescence nucleic acid detector with rapid detection function Download PDFInfo
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- CN112029655A CN112029655A CN202010898800.5A CN202010898800A CN112029655A CN 112029655 A CN112029655 A CN 112029655A CN 202010898800 A CN202010898800 A CN 202010898800A CN 112029655 A CN112029655 A CN 112029655A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention relates to a real-time fluorescent nucleic acid detector with a rapid detection function, which comprises: the device comprises a heating body assembly, a hot cover assembly, a light source excitation and fluorescence detection assembly, a motion assembly, a circuit control board, a computer and an external power supply; the heating body assembly, the heat cover assembly, the light source excitation and fluorescence detection assembly, the motion assembly and the computer are respectively connected with the circuit control board; the driver of the motion component and the computer are respectively connected with the external power supply. The real-time fluorescent nucleic acid detector can break through the situation that the existing nucleic acid detector cannot detect the wandering speed, shorten the detection time, detect more samples in one working day, reduce the waiting time of infectious disease infected patients and suspected patients, provide a diagnosis and treatment scheme for confirmed patients as soon as possible, and inhibit the spreading trend of the transmitted fever.
Description
Technical Field
The invention relates to the technical field of nucleic acid amplification detection, in particular to a real-time fluorescent nucleic acid detector with a rapid detection function.
Background
The nucleic acid detecting instrument is an instrument for amplifying and detecting nucleic acid by a PCR technique or a constant temperature amplification method, and the real-time fluorescent nucleic acid detecting instrument is an instrument for reflecting the amount of an amplified product by detecting the accumulated amount of a fluorescent signal generated by a fluorescent dye or a specific probe added to a reaction system. During constant-temperature amplification, the nucleic acid detector and the real-time fluorescent nucleic acid detector need to maintain the reaction tube at a constant temperature; during PCR amplification, the temperature of the reaction tube needs to be continuously cycled on a plurality of temperature points by the nucleic acid detector and the real-time fluorescent nucleic acid detector, the time of the constant temperature section of each temperature point depends on the size of a reagent and an amplification product of the reaction and is generally fixed, so that only the speed of temperature rise and temperature fall can be increased to increase the detection speed. The heating rate is determined by the power of the refrigerating sheet and the mass and the heat capacity of the metal block with the metal hole position groove, the higher the power of the refrigerating sheet is, the lighter the mass and the lower the heat capacity of the metal block are, the faster the heating rate is, but the higher the power of the refrigerating sheet is, the more heat is generated, the heat dissipation can be influenced, and under the condition that the mass of the metal block is light enough and the material is generally made of a low heat capacity material at present, the detection speed can be further improved only by improving the cooling speed. The existing nucleic acid detector and the real-time fluorescent nucleic acid detector mainly radiate heat generated by a refrigerating sheet to the environment as soon as possible by two modes of water cooling, radiating sheet and fan to finish the cooling process. The water-cooling heat dissipation method has the problems of high cost, complex mechanism, possible liquid leakage of cooling liquid after long-term use and the like, and the heat dissipation plate plus the fan has the advantages of simple structure, low cost and stable performance, so the heat dissipation method mainly used at present mainly comprises the heat dissipation plate plus the fan. However, the traditional nucleic acid detector and the refrigerating sheet of the heating body assembly of the real-time fluorescent nucleic acid detector complete the heat transfer process in a mode that the coated heat-conducting silicone grease is in direct contact with the radiating sheet, and because the heat conductivity coefficient of the heat-conducting silicone grease is lower, a large amount of heat generated by the heat-conducting silicone grease refrigerating sheet which is used alone is difficult to be transferred to the radiating sheet quickly, so that the cooling speed is influenced.
Disclosure of Invention
The invention aims to provide a real-time fluorescent nucleic acid detector with a rapid detection function aiming at the defects in the prior art.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a real-time fluorescent nucleic acid detector with a rapid detection function, which comprises: the device comprises a heating body assembly, a hot cover assembly, a light source excitation and fluorescence detection assembly, a motion assembly, a circuit control board, a computer and an external power supply; the heating body assembly, the heat cover assembly, the light source excitation and fluorescence detection assembly, the motion assembly and the computer are respectively connected with the circuit control board; the driver of the motion component and the computer are respectively connected with the external power supply;
the heating body assembly comprises at least one refrigerating sheet for heating and cooling and at least eight metal hole position grooves for accommodating reaction tubes;
the hot cover assembly comprises a hot cover top plate which is used for compressing the upper ends of the reaction tubes and heating the upper ends of the reaction tubes and can compress and extend;
the light source excitation and fluorescence detection assembly comprises a light source excitation box and a fluorescence detection box which are uniformly distributed on the lens cone disc, and incident optical fibers and emergent optical fibers which are uniformly distributed on the optical fiber disc;
the motion assembly comprises a motor and a driver;
the driver controls the motor to drive the lens cone disc to rotate, so that the light source excitation box of each channel is aligned with each incident optical fiber in sequence, and the fluorescence detection box of each channel is aligned with each emergent optical fiber in sequence.
Preferably, the method further comprises the following steps: the device comprises a power switch, a USB interface, a screen and a power interface; the power switch is connected with the circuit control board, the USB interface is connected with the computer, the screen is integrated with the computer, and the power interface is connected with an adapter of the external power supply.
Preferably, the device further comprises an upper shell plate, a lower shell plate, a rear shell plate and a button which are integrally formed; the upper shell plate is connected with the lower shell plate with two sides extending upwards through the side walls with two sides extending downwards; the hot cover component is arranged behind the upper shell plate and is fixed on the substrate in the lower shell plate through a left support rod and a right support rod; the power switch is arranged on the rear shell plate; the USB interfaces comprise a first USB interface and a second USB interface which are respectively arranged on the upper shell plate and the rear shell plate on one side close to the screen; the screen is arranged in front of the upper shell plate; the power supply interface is arranged on the rear shell plate; the button is located hot lid upper cover plate the place ahead and is fixed in on the epitheca board to buckle mutually through the arch of recess with hot lid base plate front end, the reaction tube of inserting in the metal hole position groove is pressed to the rethread hot lid top plate of straining, and the hot lid subassembly of straining is sealed through the ornamental strip that the lower limb contact heating body subassembly of hot lid silica gel cover then the reaction space of heating body subassembly top.
Preferably, the metal hole position grooves are formed in a long-strip-shaped metal block, the real-time fluorescent nucleic acid detector is provided with at least one and at most six long-strip-shaped metal blocks, and at most sixteen metal hole position grooves are formed in the long-strip-shaped metal blocks.
Further preferably, when the real-time fluorescent nucleic acid detector has at least two metal blocks, the metal blocks have at least 1mm space interval therebetween, the metal blocks can be further isolated by a heat insulating material, so that the metal blocks are mutually independent, an independent strip refrigerating sheet is arranged below each metal block, the installation of the optical fiber can be facilitated by the independent metal blocks and the independent refrigerating sheets, the temperature of each metal block can be independently controlled, and the reaction temperature such as annealing temperature can be optimized.
Preferably, the side wall of the metal hole position groove is provided with two through holes which are respectively connected with the incident optical fiber and the emergent optical fiber.
Preferably, the heating body assembly further comprises an air duct, a fan, a heat dissipation copper sheet, a heat conduction sheet, a heat dissipation fin and a temperature sensor; the heat conducting fins comprise a first heat conducting fin and a second heat conducting fin, and the temperature sensor comprises a first temperature sensor for measuring the temperature of the metal hole position groove and a second temperature sensor for measuring the temperature of the radiating fin; the metal block provided with the metal hole position groove is internally provided with the first temperature sensor, the metal block is provided with the first heat conducting sheet, the refrigerating sheet, the second heat conducting sheet and the heat dissipation copper sheet are sequentially laminated from top to bottom, the lower surface of the heat dissipation copper sheet is uniformly coated with heat conducting silicone grease, and the heat dissipation sheet is tightly laminated on the upper surface of the heat dissipation copper sheet and provided with the second temperature sensor.
Preferably, the heating body assembly further comprises a heating body pressing plate, a silica gel sleeve, a decorative strip and a pressing strip; when the number of the metal blocks is two or more, the heating body pressing plate comprises a front heating body pressing plate, at least one middle heating body pressing plate and a rear heating body pressing plate; when only one metal block is arranged, the heating body pressing plate comprises a front heating body pressing plate and a rear heating body pressing plate; the heating body pressing plate is provided with a plurality of threaded through holes, the radiating copper sheet and the radiating sheet are provided with corresponding threaded through holes or threaded blind holes, and screws sequentially penetrate through the threaded holes so as to tightly press the metal block, so that the metal block, the first heat conducting sheet, the strip refrigerating sheet, the second heat conducting sheet, the radiating copper sheet and the radiating sheet are kept in close contact, and meanwhile, the heat conducting sheet and the refrigerating sheet are prevented from being punched to damage the temperature uniformity and influence on the service life of the refrigerating sheet; the middle of the silica gel sleeve for heat preservation is provided with an opening and is attached to the periphery of the metal block; through holes corresponding to the metal hole position grooves in number and position are formed in the decorative strip for protecting the whole heating body assembly, and the decorative strip is attached to the upper portion of the silica gel sleeve; the pressing strip for fixing the second temperature sensor is fixed on the upper surface of the radiating fin through a through hole.
Preferably, the hot cover assembly further comprises an upper hot cover plate, a base hot cover plate, a silica gel cover and a lower hot cover plate which are sequentially arranged from top to bottom; the hot cover top plate is fixed between the hot cover base plate and the hot cover lower cover plate; four column grooves formed above the top plate of the heat cover are in contact with springs on four columns arranged below the base plate of the heat cover, and side plates extending downwards on the periphery of the top plate of the heat cover penetrate through an opening in the middle of the lower cover plate of the heat cover; the middle of the thermal cover silica gel sleeve is provided with an opening and is attached to the side plate for heat preservation and insulation.
Preferably, the light source excitation box comprises a light source box upper shell plate, a light source box lower shell plate, an LED light source and a first lens group; the first lens group comprises a first lens, a first optical filter and a second lens; the upper shell plate of the light source box is attached to the outer side of the lower shell plate of the light source box through the side walls extending downwards at the periphery; the LED light source, the first lens, the first optical filter and the second lens are sequentially arranged on the rectangular lower shell plate of the light source box.
Preferably, the fluorescence detection cassette includes a cassette upper housing plate, a cassette lower housing plate, a PD detector (i.e., a photodiode), and a second lens group; the second lens group comprises a third lens, a second optical filter and a fourth lens; the upper shell plate of the detection box is attached to the outer part of the lower shell plate of the detection box through the side walls extending downwards at the periphery; the PD detector, the fourth lens, the second optical filter and the third lens are sequentially arranged on the rectangular lower shell plate of the detection box.
Preferably, lens barrel dish is reserved have a plurality ofly with the light source arouse the box and fluorescence detects the equal slot position that corresponds of box position and base size, the light source arouse the box and fluorescence detects box slot position size unanimity, and cross sectional shape is rectangle or square, and slot position total number can be two, four, six, eight, ten or twelve to can dispose single channel, binary channels, three-channel, four-channel, five-channel or six-channel detector respectively, on the slot the light source arouse the box and fluorescence detects the box junction and can set up to fixed or pluggable structure.
By adopting the technical scheme, compared with the prior art, the invention has the following technical effects:
the real-time fluorescent nucleic acid detector can break through the situation that the existing nucleic acid detector cannot detect the wandering speed, shorten the detection time, detect more samples in one working day, reduce the waiting time of infectious disease infected patients and suspected patients, provide a diagnosis and treatment scheme for confirmed patients as soon as possible, and inhibit the spreading trend of the transmitted fever. The rectangular light source box and the rectangular detection box are adopted, the light source box and the detection box are simple in assembly and universal in function, different optical filters can be replaced to adapt to different channels, and the used scene can be expanded by adding different optical elements.
Drawings
FIG. 1 is a perspective view of a real-time fluorescent nucleic acid detecting apparatus according to the present invention;
FIG. 2 is a view showing the internal structure of the real-time fluorescent nucleic acid detecting apparatus according to the present invention;
FIG. 3 is a view showing the assembly of the housing of the real-time fluorescent nucleic acid detecting apparatus according to the present invention;
FIG. 4 is an exploded view of a thermal cap assembly of the instant fluorescence nucleic acid detector;
FIG. 5 is an exploded view of a heater assembly of the real-time fluorescent nucleic acid detector of the present invention;
FIG. 6 is an exploded view of a heater assembly of the real-time fluorescent nucleic acid detector of the present invention;
FIG. 7 is an exploded view of a heater assembly of the real-time fluorescent nucleic acid detector of the present invention;
FIG. 8 is an exploded view of a prior art heater block assembly;
FIG. 9 is a reverse exploded view and a forward assembled view of the light source excitation and fluorescence detection assembly and motor of the real-time fluorescent nucleic acid detector of the present invention;
FIG. 10 is a cross-sectional view and an assembly view of the light source excitation and fluorescence detection assembly of the real-time fluorescent nucleic acid detector of the present invention;
wherein the reference numerals include: a heating body assembly 1; a refrigerating sheet 11; a metal block 12; a metal hole site groove 121; metal hole trench sidewall vias 122; an air duct 13; a fan 14; a heat dissipating copper sheet 15; a heat conductive sheet 16; a first thermally conductive sheet 161; a second thermally conductive sheet 162; a heat sink 17; a temperature sensor 18; a first temperature sensor 181; a second temperature sensor 182; heat conductive silicone grease 19; a heating body pressing plate 110; a front heating body platen 1101; a medium heating body pressing plate 1102; a rear heating body platen 1103; a silica gel cover 111; a decorative strip 112; a bead 113; a hot lid assembly 2; a hot lid top plate 21; a column groove 211; side panels 212 (extending below the top panel of the hot header); a hot lid upper cover plate 22; a thermal cover substrate 23; a column 231; a spring 232; a bump 233 (at the front of the thermal cover substrate); a hot lid silicone cover 24; a hot lid lower cover plate 25; a light source excitation and fluorescence detection assembly 3; a lens barrel disk 31; a light source excitation box 32; a light source box upper case plate 3201; a light source case lower plate 3202; an LED light source 321; a first lens group 322; a first lens 3221; a first filter 3222; a second lens 3223; a fluorescence detection cassette 33; a cartridge upper housing plate 3301; a cartridge lower housing plate 3302; a detector 331; a second lens group 332; a third lens 3321; a second filter 3322; a fourth lens 3323; a fiber optic plate 34; a photosensor 35; a motor 41; a driver 42; a circuit control board 5; a power switch 51; a first USB interface 61; a screen 62; a second USB interface 63; an external power supply 7; a power supply interface 71; an upper shell plate 81; a sidewall 811 (of the upper housing plate); a lower shell plate 82; left and right support rods 821; a substrate 822; a rear shell plate 83; a button 84; a button recess 841.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
Example 1
The present embodiment provides a real-time fluorescent nucleic acid detector with rapid detection function, as shown in fig. 1 to 3, including: the device comprises a heating body assembly 1, a hot cover assembly 2, a light source excitation and fluorescence detection assembly 3, a motion assembly, a circuit control board 5, a computer and an external power supply 7; the heating body assembly 1, the hot cover assembly 2, the light source excitation and fluorescence detection assembly 3, the motion assembly and the computer are respectively connected with the circuit control board 5; the driver 42 of the motion component and the computer are respectively connected with the external power supply 7;
the heating body assembly 1 comprises at least one refrigerating sheet 11 for heating and cooling and at least eight metal hole position grooves 121 for accommodating reaction tubes;
the hot cap assembly 2 includes a hot cap plate 21 for compressing and heating the upper ends of the reaction tubes and performing compression and expansion movements;
the light source excitation and fluorescence detection assembly 3 comprises a light source excitation box 32 and a fluorescence detection box 33 which are uniformly distributed on a lens barrel disc 31, and an incident optical fiber and an emergent optical fiber which are uniformly distributed on an optical fiber disc 34;
the moving assembly comprises a motor 41 and a driver 42,
the driver 42 controls the motor 41 to drive the lens barrel disc 31 to rotate, so that the light source excitation box 32 of each channel is aligned with each incident optical fiber in sequence, and the fluorescence detection box 33 of each channel is aligned with each emergent optical fiber in sequence.
Preferably, the method further comprises the following steps: a power switch 51, a USB interface, a screen 62, and a power interface 71; the power switch 51 is connected with the circuit control board 5, the USB interface is connected with the computer, the screen 62 is integrated with the computer, and the power interface 71 is connected with the adapter of the external power supply 7.
As shown in fig. 3, it preferably further includes an upper shell plate 81, a lower shell plate 82, a rear shell plate 83 and a button 84 which are integrally formed; the upper shell plate 81 is connected to the lower shell plate 82 with two sides extending upwards through two side walls 811 extending downwards; the heat cover assembly 2 is disposed behind the upper shell plate 81 and fixed to a base plate 822 in the lower shell plate 82 through left and right support rods 821; the power switch 51 is arranged on the rear shell plate 83; the USB interfaces include a first USB interface 61 and a second USB interface 63, which are respectively disposed on the upper shell plate 81 and the rear shell plate 83 near one side of the screen 62; the screen 62 is arranged in front of the upper shell plate 81; the power interface 71 is arranged on the rear shell plate 83; the button 84 is located in front of the upper cover plate 22 and fixed on the upper shell plate 81, and is engaged with the protrusion 233 at the front end of the heat cover base plate 23 through the groove 841.
Therefore, the whole instrument has a firm structure and is convenient to assemble; the two USB interfaces are adopted, so that the USB interface arranged in front of the instrument can be conveniently used for plugging a USB flash disk, the content displayed on a screen can be checked, data and pictures can be copied, a mouse or a keyboard can be conveniently connected when the front USB flash disk is occupied, and screen content can be conveniently selected or operation parameters can be conveniently input; when carrying out the amplification reaction, the hot lid can be closed to the one hand lower pressure hot lid upper cover plate 22, can shelter from ambient light through hot lid upper cover plate 22, can play the effect that prevents heating back reaction tube upper portion expansion or reaction liquid evaporation through hot lid roof 21, can further play the effect that prevents evaporation and condensation through the heating of hot lid roof 21, after the reaction, through pressing button 84, can be convenient open the hot lid, take out the reaction tube.
The working principle is as follows: the sample to be measured in the reaction tube reacts at a constant temperature or a plurality of temperatures controlled by the heating body component 1, when light emitted by the light source irradiates the sample to be measured through the incident optical fiber, if an amplification product is generated, the accumulated fluorescence enters the detector through the emergent optical fiber to form a fluorescence signal, and the fluorescence signal is analyzed by the computer and then displayed through the screen 62.
Preferably, the metal hole position groove 121 is opened on the strip-shaped metal block 12, the real-time fluorescent nucleic acid detector is provided with at least one and at most six metal holes 121 on the strip-shaped metal block 12, and the strip-shaped metal block 12 is opened with at most sixteen metal hole position grooves 121.
Preferably, two through holes 122 are formed in the side wall of the metal hole position slot 121, and are respectively connected to the incident optical fiber and the emergent optical fiber.
Preferably, the heating body assembly 1 further comprises an air duct 13, a fan 14, a heat dissipation copper sheet 15, a heat conduction sheet 16, a heat dissipation sheet 17 and a temperature sensor 18; the heat-conducting fins 16 include a first heat-conducting fin 161 and a second heat-conducting fin 162, and the temperature sensor 18 includes a first temperature sensor 181 for measuring the temperature of the metal hole site groove 121 and a second temperature sensor 182 for measuring the temperature of the heat sink 17; the metal block 12 provided with the metal hole position groove 121 is internally provided with the first temperature sensor 181, the metal block 12 is sequentially attached to the first heat conducting sheet 161, the refrigerating sheet 11, the second heat conducting sheet 162 and the heat dissipation copper sheet 15 from top to bottom, the lower surface of the heat dissipation copper sheet 15 is uniformly coated with heat conduction silicone grease 19, and the heat dissipation sheet 17 tightly attached to the upper surface of the heat dissipation copper sheet 182 is provided with the second temperature sensor 182.
Preferably, the heating body assembly 1 further comprises a heating body pressing plate 110, a silica gel sleeve 111, a decorative strip 112 and a pressing strip 113; when there are two or more metal blocks 12, the heating body pressing plate 110 includes a front heating body pressing plate 1101, at least one middle heating body pressing plate 1102, and a rear heating body pressing plate 1103; when there is only one metal block 12, the heating body pressing plate 110 includes a front heating body pressing plate 1101 and a rear heating body pressing plate 1103; a plurality of threaded through holes are formed in the heating body pressing plate 110, and corresponding threaded through holes or threaded blind holes are formed in the heat dissipation copper sheet 15 and the heat dissipation fins 17; the middle of the silica gel sleeve 111 for heat preservation is opened and is attached to the periphery of the metal block 12; through holes corresponding to the number and positions of the metal hole position grooves 121 are formed in the decorative strip 112 for protecting the whole heating body assembly 1, and the decorative strip is attached to the upper part of the silica gel sleeve 111; the compression bar 113 for fixing the second temperature sensor 182 is fixed to the upper surface of the heat sink 17 through a through hole.
As shown in fig. 5 to 7, during the amplification reaction, after a sample to be measured is added into the reaction tube, the reaction tube is placed in the metal hole position groove 121, the upper surface of the cooling fin 11 starts to be heated, the heat is rapidly and uniformly transferred to the metal block 12 through the first heat-conducting fin 161, the sample solution in the reaction tube is heated, the first temperature sensor 181 detects the temperature of the metal hole position groove 121, the power of the cooling fin 11 is controlled by the circuit control board 5 after being fed back to the computer, and the stability of the sample temperature during the reaction is further maintained. When will reduce the sample liquid temperature, refrigeration piece 11 upper surface cooling, the instantaneous large amount of heats of production of lower surface simultaneously, second piece conducting strip 162 with the help of refrigeration piece 11 below can be rapidly with the heat transfer for heat dissipation copper sheet 15 of semiconductor piece 11 accumulation, because the area of the copper sheet that uses is greater than the area of second piece conducting strip 162, the copper sheet can play and hold and cushion thermal effect, the heat of copper sheet has sufficient time to transmit for fin 17, the system relies on fan 14 again to take the heat out of the instrument through wind channel 13. It can be seen that the occupied volume of the heating body assembly is not obviously changed compared with the prior art (fig. 8), but the heat transfer efficiency is higher and the heat dissipation performance is better.
As shown in fig. 4, the hot lid assembly 2 preferably further includes an upper hot lid cover plate 22, a base hot lid plate 23, a silicone hot lid cover 24, and a lower hot lid cover plate 25, which are arranged in sequence from top to bottom; the hot cover top plate 21 is fixed between the hot cover base plate 23 and the hot cover lower cover plate 25; four column grooves 211 formed in the upper portion of the hot cover top plate 21 are in contact with springs 232 on four columns 231 arranged below the hot cover base plate 23, and side plates 212 extending downward around the hot cover top plate 21 penetrate through an opening in the middle of the hot cover lower cover plate 25; the middle of the thermal cover silica gel sleeve 24 for heat preservation and insulation is opened and is attached to the side plate 212.
As shown in fig. 10, preferably, the light source excitation case 32 includes a light source case upper casing plate 3201, a light source case lower casing plate 3202, an LED light source 321, and a first lens group 322; the first lens group 322 includes a first lens 3221, a first optical filter 3222, and a second lens 3223 sequentially arranged along an incident light path of the optical fiber; the light source box upper casing plate 3201 is attached to the outside of the light source box lower casing plate 3202 through side walls extending downwards at the periphery; the LED light source 321, the first lens 3221, the first filter 3222, and the second lens 3223 are sequentially mounted on the rectangular light source box lower casing 3202.
The LED light sources can all adopt monochromatic LEDs, can be purple light LEDs, blue light LEDs, green light LEDs, yellow light LEDs and red light LEDs, and can also all adopt white light LEDs; the first lens 3221 has a collimating effect, the second lens 3223 has a light-condensing effect, the lenses may be PMMA lenses, PC lenses, resin lenses, silica gel lenses or optical glass lenses, and the optical filter is a narrow-band optical filter.
Preferably, the fluorescence detection cassette 33 includes a detection cassette upper case plate 3301, a detection cassette lower case plate 3302, a PD detector 331, and a second lens group 332; the second lens group 332 comprises a third lens 3321, a second optical filter 3322 and a fourth lens 3323 which are sequentially arranged along an optical fiber emergent light path; the upper detection box shell plate 3301 is attached to the outer side of the lower detection box shell plate 3302 through the side walls extending downwards; the PD detector 331, the fourth lens 3323, the second filter 3322, and the third lens 3321 are sequentially mounted on the rectangular lower case plate 3302 of the measuring cassette.
The PD detector can also be replaced by an avalanche photo diode APD (avalanche photo diode), and can be a silicon avalanche photo diode SiAPD or a vacuum avalanche photo diode VAPD, a photo IC (integrated circuit), a multi-pixel photon counter MPPC (maximum power point tracking), a photomultiplier tube PMT (photomultiplier tube), a photoelectric tube, a linear array image sensor, an area array image sensor and the like; the third lens 3321 and the fourth lens 3323 have a light-gathering effect, the lenses can be PMMA lenses, PC lenses, resin lenses, silica gel lenses or optical glass lenses according to the material, the optical filter is a narrow band filter, and the optical filter of the last channel can be a narrow band filter or a long-wave pass filter.
The present embodiment takes the fluorescence detection box 33 as an example, and the structures of the light source excitation box 32 and the fluorescence detection box 33 will be further described. Fluorescence detection box 33 is the rectangle, closes as an organic wholely through tenon fourth of the twelve earthly branches structure, screw fixation or veneer mode by last coverboard 3301 and lower coverboard 3302, and the structure that rectangle casing and last coverboard and lower coverboard part are divided compares traditional cylinder and integration has the advantage of being convenient for process, be convenient for place each optical element, be convenient for assemble, occupation space is littleer. For convenience of installation and fixation, the lens and the optical filter in the rectangular fluorescence detection box 33 are processed into a square shape or a rectangular shape, two opposite sides or four sides of the lens are provided with protruding edges, the edges are long-strip square shapes and are used for being inserted into the groove of the fluorescence detection box 33 for fixation, the edges can also be wedge-shaped and are used for being embedded into the conical channel of the fluorescence detection box 33 for fixation, and a plurality of groups of cylinders, springs, movable plates, gaskets or gaskets can be arranged in the fluorescence detection box 33 for further fixation of the lens and the optical filter. An optical fiber clamping groove or a thread groove can be reserved at the entrance of the fluorescence detection box 33 and used for directly connecting the emergent optical fiber without the help of the optical fiber disc 34 and the lens cone disc 31. A plurality of grooves or tapered channels can be reserved in the fluorescence detection box 33, so that optical elements such as a polarizing film or an attenuation film can be replaced or added, and the requirements of fluorescence detection and the requirements of expansion of the use scene of the instrument can be met more flexibly.
As shown in fig. 9, when light source excitation and fluorescence detection are performed, the light source excitation box 32 and the fluorescence detection box 33 of the present invention are installed on the lens barrel tray 31, the incident optical fiber and the emergent optical fiber are installed on the optical fiber tray 34, the lens barrel tray 31 is driven by the motor 41 to rotate relative to the optical fiber tray, so that the light source excitation box 32 of each channel is aligned with each incident optical fiber in sequence, the fluorescence detection box 33 of each channel is aligned with each emergent optical fiber in sequence, the detection of multiple channels can be completed when the lens barrel tray 31 runs for one circle, and the position of the lens barrel tray 31 can be accurately positioned by the photoelectric sensor 35. Therefore, only one motor 41 is needed by the motion assembly, the cost is saved, and the motion parts are reduced, so that the stability of the system is improved.
As shown in fig. 9, preferably, a plurality of slot positions corresponding to the positions of the light source excitation box and the fluorescence detection box and the size of the bottom edge are reserved on the lens barrel disc, the slot positions of the light source excitation box and the fluorescence detection box are consistent in size, the cross section of each slot position is rectangular or square, the total number of the slot positions can be two, four, six, eight, ten or twelve, so that single-channel, double-channel, three-channel, four-channel, five-channel or six-channel detectors can be configured respectively, and the joints of the light source excitation box and the fluorescence detection box on the slots can be set to be fixed or pluggable structures. So, when needs maintenance, only need to change corresponding light source box or detect the box can. By adding the light source box and the detection box in pairs, the instrument can be conveniently upgraded into a detector with more channels.
The novel heating body component structure is introduced into the real-time fluorescent nucleic acid detector, so that the situation that the existing nucleic acid detector cannot detect the speed of wandering can be broken through, the detection time is shortened, more samples can be detected within one working day, the waiting time of infectious disease infected patients and suspected patients is reduced, a diagnosis and treatment scheme is provided for confirmed patients as soon as possible, and the spreading trend of the transmitted heat disease is restrained. The rectangular light source box and the rectangular detection box are adopted, the light shading performance and the structural stability of the optical component are improved, the light source box and the detection box are simple in assembly, universal in function and easy to process and assemble, different optical filters can be replaced to adapt to different channels, and the used scenes can be expanded by adding different optical elements.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.
Claims (10)
1. A real-time fluorescent nucleic acid detector with rapid detection function, comprising: the device comprises a heating body assembly (1), a hot cover assembly (2), a light source excitation and fluorescence detection assembly (3), a movement assembly, a circuit control board (5), a computer and an external power supply (7); the heating body assembly (1), the hot cover assembly (2), the light source excitation and fluorescence detection assembly (3), the motion assembly and the computer are respectively connected with the circuit control board (5); the driver (42) of the motion component and the computer are respectively connected with the external power supply (7);
the heating body assembly (1) comprises at least one refrigerating sheet (11) for heating and cooling and at least eight metal hole position grooves (121) for accommodating reaction tubes;
the hot cover assembly (2) comprises a hot cover top plate (21) which is used for compressing the upper ends of the reaction tubes and heating the reaction tubes and can compress and stretch;
the light source excitation and fluorescence detection assembly (3) comprises a light source excitation box (32) and a fluorescence detection box (33) which are uniformly distributed on a lens barrel disc (31), and incident optical fibers and emergent optical fibers which are uniformly distributed on an optical fiber disc (34);
the motion assembly comprises a motor (41) and a driver (42).
2. The real-time fluorescent nucleic acid detector of claim 1, further comprising: a power switch (51), a USB interface, a screen (62), and a power interface (71); the power switch (51) is connected with the circuit control board (5), the USB interface is connected with the computer, the screen (62) is integrated with the computer, and the power interface (71) is connected with an adapter of the external power supply (7).
3. The real-time fluorescent nucleic acid detecting instrument according to claim 1 or 2, further comprising an upper shell plate (81), a lower shell plate (82), a rear shell plate (83), and a button (84) which are integrally formed; the upper shell plate (81) is connected with the lower shell plate (82) with two sides extending upwards through the side walls (811) with two sides extending downwards; the hot cover assembly (2) is arranged behind the upper shell plate (81) and is fixed on a substrate (822) in the lower shell plate (82) through a left support rod and a right support rod (821); the power switch (51) is arranged on the rear shell plate (83); the USB interfaces comprise a first USB interface (61) and a second USB interface (63) which are respectively arranged on the upper shell plate (81) and the rear shell plate (83) which are close to one side of the screen (62); the screen (62) is arranged in front of the upper shell plate (81); the power supply interface (71) is arranged on the rear shell plate (83); the button (84) is positioned in front of the upper cover plate (22) of the heat cover, fixed on the upper shell plate (81), and buckled with a bulge (233) at the front end of the heat cover substrate (23) through a groove (841).
4. The real-time fluorescent nucleic acid detector according to claim 1, wherein the metal hole position groove (121) is opened on a strip-shaped metal block (12), the real-time fluorescent nucleic acid detector is provided with at least one and at most six of the strip-shaped metal blocks (12), and at most sixteen metal hole position grooves (121) are opened on the strip-shaped metal block (12).
5. The real-time fluorescent nucleic acid detector of claim 1, wherein two through holes (122) are opened on the side wall of the metal hole slot (121) and respectively connected to the incident optical fiber and the emergent optical fiber.
6. The real-time fluorescent nucleic acid detector according to claim 1, wherein the heating body assembly (1) further comprises an air duct (13), a fan (14), a heat-dissipating copper sheet (15), a heat-conducting sheet (16), a heat-dissipating fin (17), and a temperature sensor (18); the heat conducting sheet (16) comprises a first heat conducting sheet (161) and a second heat conducting sheet (162), and the temperature sensor (18) comprises a first temperature sensor (181) for measuring the temperature of the metal hole position groove (121) and a second temperature sensor (182) for measuring the temperature of the heat radiating fin (17); seted up the metal block (12) inside of metal hole position groove (121) is equipped with first temperature sensor (181), metal block (12) first piece conducting strip (161) refrigeration piece (11) second piece conducting strip (162) and heat dissipation copper sheet (15) from last to laminating in proper order down, heat dissipation copper sheet (15) lower surface evenly coated has heat conduction silicone grease (19), and closely laminate and be equipped with in the upper surface second temperature sensor (182) fin (17).
7. The real-time fluorescent nucleic acid detector of claim 6, wherein the heating body assembly (1) further comprises a heating body pressing plate (110), a silicone sleeve (111), a decorative strip (112) and a pressing strip (113); when the number of the metal blocks (12) is two or more, the heating body pressing plate (110) comprises a front heating body pressing plate (1101), at least one middle heating body pressing plate (1102) and a rear heating body pressing plate (1103); when there is only one metal block (12), the heating body pressing plate (110) comprises a front heating body pressing plate (1101) and a rear heating body pressing plate (1103); a plurality of threaded through holes are formed in the heating body pressing plate (110), and corresponding threaded through holes or threaded blind holes are formed in the heat dissipation copper sheet (15) and the heat dissipation fin (17); the middle of the silica gel sleeve (111) for heat preservation is opened and is attached to the periphery of the metal block (12); through holes corresponding to the metal hole position grooves (121) in number and positions are formed in the decorative strip (112) for protecting the whole heating body assembly (1), and the decorative strip is attached to the upper portion of the silica gel sleeve (111); the pressing strip (113) for fixing the second temperature sensor (182) is fixed on the upper surface of the heat radiating fin (17) through a through hole.
8. The real-time fluorescent nucleic acid detecting instrument according to claim 1, wherein the hot lid assembly (2) further comprises a hot lid upper cover plate (22), a hot lid base plate (23), a hot lid silicone sleeve (24) and a hot lid lower cover plate (25) arranged in this order from top to bottom; the hot cover top plate (21) is fixed between the hot cover base plate (23) and the hot cover lower cover plate (25); four column grooves (211) formed in the upper portion of the hot cover top plate (21) are in contact with springs (232) on four upright columns (231) arranged below the hot cover base plate (23), and side plates (212) extending downwards on the periphery of the hot cover top plate (21) penetrate through an opening in the middle of the hot cover lower cover plate (25); the middle of the heat cover silica gel sleeve (24) for heat preservation and heat insulation is opened and is attached to the side plate (212).
9. The real-time fluorescent nucleic acid detecting instrument according to claim 1, wherein the light source excitation cartridge (32) includes a light source cartridge upper case plate (3201), a light source cartridge lower case plate (3202), an LED light source (321), and a first lens group (322); the first lens group (322) comprises a first lens (3221), a first optical filter (3222) and a second lens (3223); the upper shell plate (3201) of the light source box is attached to the outer side of the lower shell plate (3202) of the light source box through side walls extending downwards at the periphery; the LED light source (321), the first lens (3221), the first optical filter (3222) and the second lens (3223) are sequentially mounted on the rectangular light source box lower shell plate (3202).
10. The real-time fluorescent nucleic acid detecting instrument according to claim 1, wherein the fluorescence detecting cassette (33) includes a cassette upper case plate (3301), a cassette lower case plate (3302), a PD detector (331), and a second lens group (332); the second lens group (332) includes a third lens (3321), a second filter (3322), and a fourth lens (3323); the upper shell plate (3301) of the detection box is attached to the outer side of the lower shell plate (3302) of the detection box through the side walls extending downwards at the periphery; the PD detector (331), the fourth lens (3323), the second filter (3322) and the third lens (3321) are sequentially mounted on the rectangular detection box lower shell plate (3302).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010898800.5A CN112029655A (en) | 2020-08-31 | 2020-08-31 | Real-time fluorescence nucleic acid detector with rapid detection function |
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| CN202010898800.5A CN112029655A (en) | 2020-08-31 | 2020-08-31 | Real-time fluorescence nucleic acid detector with rapid detection function |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110656046A (en) * | 2019-11-01 | 2020-01-07 | 中国人民解放军军事科学院军事医学研究院 | Detection device and PCR instrument |
| CN112779151A (en) * | 2021-03-02 | 2021-05-11 | 济南国益生物科技有限公司 | Portable fluorescent quantitative nucleic acid amplification instrument |
| CN112779150A (en) * | 2021-03-02 | 2021-05-11 | 济南国益生物科技有限公司 | Constant temperature amplification nucleic acid detector |
| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
-
2020
- 2020-08-31 CN CN202010898800.5A patent/CN112029655A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110656046A (en) * | 2019-11-01 | 2020-01-07 | 中国人民解放军军事科学院军事医学研究院 | Detection device and PCR instrument |
| CN112779151A (en) * | 2021-03-02 | 2021-05-11 | 济南国益生物科技有限公司 | Portable fluorescent quantitative nucleic acid amplification instrument |
| CN112779150A (en) * | 2021-03-02 | 2021-05-11 | 济南国益生物科技有限公司 | Constant temperature amplification nucleic acid detector |
| CN113049564A (en) * | 2021-03-26 | 2021-06-29 | 江苏宏微特斯医药科技有限公司 | Fluorescence detection device |
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