CN212560271U - Amplification device applied to nucleic acid detection - Google Patents

Abstract

The utility model provides a be applied to nucleic acid testing's amplification device, this be applied to nucleic acid testing's amplification device includes: the device comprises a nucleic acid detection sheet, a temperature changing module, an optical detection module, a motor driving module and a shell; the temperature-changing module is arranged at the bottom of the shell, the nucleic acid detection sheet is arranged above the temperature-changing module, the optical detection module is arranged above the nucleic acid detection sheet, and the motor driving module is arranged above the optical detection module; the nucleic acid detection sheet is used for placing a reagent and a sample to be detected; the temperature changing module is used for providing a temperature environment required by the reaction according to the reaction condition; the optical detection module is used for providing a detection light source for the sample to be detected and detecting fluorescence emitted by the sample to be detected; and the motor driving module is used for driving the optical detection module to move so as to detect the samples to be detected at different positions in the nucleic acid detection sheet. The utility model discloses can reduce the volume of being applied to nucleic acid detection's amplification device.

Description

Amplification device applied to nucleic acid detection

Technical Field

The utility model relates to a nucleic acid testing technical field, in particular to be applied to nucleic acid testing's amplification device.

Background

Under the frequent circumstances of epidemic diseases, human genetic diseases, animal epidemic diseases and the like, an amplification device applied to nucleic acid detection becomes an indispensable detection and diagnosis instrument.

In practical application, a large amount of nucleic acid detection processes need to be completed on site, most of the currently mainstream amplification devices amplify samples in a reaction tube by taking the reaction tube as a carrier, the liquid reagent needs to be transported in a cold chain manner in the amplification mode, and a reaction system is temporarily configured on site, so that inconvenience is brought to detection, and the risk of pollution exists. In addition, the amplification method has low heat conduction efficiency between the reaction tube and the heat source, so that the whole amplification time is long. More importantly, the large-flux tubular amplification device is large in size and not easy to carry.

SUMMERY OF THE UTILITY MODEL

The utility model provides an amplification device for nucleic acid detection can reduce amplification device's volume.

The embodiment of the utility model provides an amplification device for nucleic acid detection, which comprises a shell, a temperature changing module, an optical detection module, a motor driving module and a nucleic acid detection sheet;

the temperature changing module, the optical detection module and the motor driving module are accommodated in the shell, the shell is provided with a plug-in sheet opening, and the nucleic acid detection sheet can be inserted into the plug-in sheet opening;

the temperature-changing module is arranged at the bottom of the shell, the nucleic acid detection sheet is arranged above the temperature-changing module, the optical detection module is arranged above the nucleic acid detection sheet, and the motor driving module is arranged above the optical detection module;

the nucleic acid detection sheet is used for placing a nucleic acid reagent and a sample to be detected;

the temperature changing module is used for providing a temperature environment required by the reaction according to the condition of the reaction between the nucleic acid reagent and the sample to be detected;

the optical detection module is used for providing a detection light source for the nucleic acid detection process and detecting fluorescence emitted by the nucleic acid detection sheet at different positions;

the motor driving module is used for driving the optical detection module to move so as to detect samples to be detected at different positions in the nucleic acid detection sheet.

In one possible design, the optical detection module includes: the device comprises a light source, a first lens, a first optical filter, a dichroic color separation sheet, a second lens, a second optical filter, a third lens and a photoelectric detector;

the first lens is used for converting the light emitted by the light source into parallel light;

the first optical filter is used for filtering light which cannot enable the sample to be detected to generate fluorescence in the parallel light after passing through the first lens;

the dichroic color separation sheet is used for changing the light path of the parallel light after passing through the first optical filter so as to reflect the light path to the nucleic acid detection sheet, and is also used for transmitting the fluorescence passing through the second lens to the photoelectric detector;

the second lens is used for focusing the light reflected by the dichroic sheet on the nucleic acid detection sheet and converting the fluorescence emitted by the nucleic acid detection sheet into parallel light;

the second optical filter is used for removing interference light except the fluorescence characteristic wavelength;

the third lens is used for focusing the light passing through the second optical filter onto the photoelectric detector;

the light source, the first lens, the first optical filter, the dichroic filter and the second lens are sequentially arranged along the light path of the light source so as to irradiate the light emitted by the light source onto the nucleic acid detection sheet;

the second lens, the dichroic filter, the second optical filter, the third lens and the photoelectric detector are sequentially arranged along the light path of the fluorescence, so that the fluorescence is detected by the photoelectric detector.

In one possible design, the temperature change module includes: a heat source, a heat dissipation fin, a radiator and heat conducting metal;

the heat source is used for providing a temperature environment required by the reaction for the nucleic acid detection process;

the heat radiating fins are arranged outside the heat source, the radiator is arranged outside the heat source, and the heat conducting metal is arranged between the heat source and the nucleic acid detecting piece;

and/or the presence of a gas in the gas,

the first lens, the second lens and the third lens are all aspheric short-focus lenses.

In one possible design, the nucleic acid detection strip includes: the detection piece comprises a detection piece body, a first closing body and a cover plate;

the detection sheet body and the first closing body are both of sheet structures;

the first side of the detection sheet body is provided with at least one sample injection hole and at least one exhaust hole, the second side of the detection sheet body is provided with at least one first flow channel groove, at least one second flow channel groove and at least one groove, and the first closing body is arranged on the second side of the detection sheet body so as to surround the detection sheet body to form at least one first flow channel, at least one second flow channel and at least one cavity;

the at least one sample injection hole, the at least one first flow channel, the at least one chamber, the at least one second flow channel and the at least one vent hole are communicated in sequence;

the at least one chamber is used for placing nucleic acid detection reagents;

a sample to be detected can enter the at least one chamber through the at least one injection hole and the at least one first flow channel to react with the nucleic acid detection reagent;

the cover plate is fixed with the first side of the detection sheet body to cover all the sample injection holes and all the exhaust holes.

In one possible design, the nucleic acid detecting sheet further includes: a second enclosure;

the second sealing body is arranged on the first side of the detection sheet body to cover all the exhaust holes;

the second closure includes a waterproof, breathable membrane.

And/or the presence of a gas in the gas,

the nucleic acid detecting sheet further comprises: a soft rubber cushion;

the apron with the first side of soft cushion is fixed, the second side of soft cushion with the first side of detecting the piece body is fixed to cover in whole notes appearance hole and whole exhaust hole.

In one possible design, the detection sheet body has sixteen injection holes, sixteen first flow channel grooves, sixteen second flow channel grooves and sixteen exhaust holes, and each injection hole is communicated with one of the first flow channel grooves, one of the second flow channel grooves and one of the exhaust holes;

or the like, or, alternatively,

the detection sheet body is provided with one sample injection hole, twenty first flow channel grooves, twenty second flow channel grooves and twenty exhaust holes, and the sample injection hole is respectively communicated with the twenty first flow channel grooves, the twenty second flow channel grooves (14) and the twenty exhaust holes;

or the like, or, alternatively,

the detection piece body is provided with four sample injection holes, sixteen first flow channel grooves, sixteen second flow channel grooves and sixteen exhaust holes, wherein each sample injection hole is communicated with four of the first flow channel grooves, four of the second flow channel grooves and four of the exhaust holes.

In one possible design, a first side of the detection sheet body is provided with an installation groove, and the cover plate is fixed in the installation groove;

the apron with one in the mounting groove is provided with the buckle, and another is provided with joint portion, the buckle with joint portion joint.

In a possible design, the first side of the detection piece body is further provided with a groove, and the groove is arranged on the inner side of the mounting groove and on the peripheries of all the sample injection holes and all the exhaust holes.

In one possible design, the test strip body, the first closure body and the cover plate are all made of plastic material;

and/or the presence of a gas in the gas,

the thickness of the first closing body along the direction of the first side of the detection sheet body pointing to the second side of the detection sheet body is 0.01mm-0.2 mm;

and/or the presence of a gas in the gas,

the groove is a structure with two ends gradually expanding towards the middle.

According to the technical scheme, the amplification device applied to nucleic acid detection mainly comprises a shell, a temperature changing module, an optical detection module, a motor driving module, a nucleic acid detection sheet and the like. The nucleic acid detection piece is arranged above the temperature change module, so that heat can be transferred to the nucleic acid detection piece from the temperature change module, the heat conduction efficiency between the temperature change module and a sample to be detected is improved, the amplification time of the sample to be detected is shortened, the volume of the temperature change module can be developed according to the area of the nucleic acid detection piece, and the temperature change module can be guaranteed to have the minimum volume on the premise of meeting the temperature change of the sample to be detected, so that the nucleic acid detection piece is convenient to carry. In addition, the motor driving module is arranged on the optical detection module through the two supports, so that the purpose of detecting different positions of the nucleic acid detection sheet by driving the optical detection module through the motor driving module can be achieved, and the space can be fully utilized, so that the amplification device is more compact and smaller in size. Therefore, the amplification device applied to nucleic acid detection has the advantages of compact structure, small volume and convenient carrying, and can realize the purpose of nucleic acid detection on site.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of an amplification apparatus for nucleic acid detection according to an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of a housing of an amplification apparatus for nucleic acid detection according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of an optical detection module according to an embodiment of the present invention;

fig. 4 is a schematic diagram of an optical path inside an optical detection module according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of a temperature change module according to an embodiment of the present invention;

FIG. 6 is an exploded view of a nucleic acid detecting piece according to an embodiment of the present invention;

FIG. 7 is a schematic structural view of a nucleic acid detecting piece body provided in an embodiment of the present invention at a viewing angle;

FIG. 8 is a schematic structural view of a nucleic acid detecting piece body provided in an embodiment of the present invention at another viewing angle;

FIG. 9 is a schematic view of a nucleic acid detecting piece body provided in another embodiment of the present invention, viewed from a single angle;

FIG. 10 is a schematic view of a nucleic acid detecting piece body provided in another embodiment of the present invention, viewed from another angle;

FIG. 11 is a schematic view of a nucleic acid detecting piece body according to still another embodiment of the present invention, viewed from a single angle;

FIG. 12 is a schematic view of a nucleic acid detecting piece body according to another embodiment of the present invention, viewed from another angle.

Detailed Description

In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the following will combine the drawings in the embodiments of the present invention to clearly and completely describe the technical solution in the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, rather than all embodiments, based on the embodiments in the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the scope of the present invention.

As shown in fig. 1, one embodiment of the present invention provides an amplification device for nucleic acid detection, which includes a temperature changing module 20, an optical detection module 30, a motor driving module 40, and a nucleic acid detection chip 10; the temperature-changing module 20 is arranged at the bottom of the shell 50, the nucleic acid detection sheet 10 is arranged above the temperature-changing module 20, the optical detection module 30 is arranged above the nucleic acid detection sheet 10, and the motor driving module 40 is arranged above the optical detection module 30;

as shown in fig. 2, the temperature changing module 20, the optical detection module 30, and the motor drive module 40 are accommodated in the housing 50, the housing 50 is provided with a card insertion opening 51, and the nucleic acid detecting card 10 can be inserted into the card insertion opening 51.

The embodiment of the utility model provides an in, set up nucleic acid detection piece 10 in the top of alternating temperature module 20, not only be favorable to transmitting the heat for nucleic acid detection piece 10 from alternating temperature module 20, promote alternating temperature module 20 and wait to detect the heat conduction efficiency between the sample, thereby reduce the amplification time of waiting to detect the sample, and alternating temperature module 20's volume can be according to the area development of nucleic acid detection piece 10, thereby can guarantee alternating temperature module 20 and wait to detect under the prerequisite of sample temperature change, have minimum volume, so that carry. In addition, the motor driving module 40 is arranged on the optical detection module 30 through two brackets, so that the purpose of detecting different positions of the nucleic acid detection sheet by driving the optical detection module 30 through the motor driving module 40 can be achieved, and the space can be fully utilized, so that the amplification device is more compact and smaller in size. Therefore, the amplification device applied to nucleic acid detection has the advantages of compact structure, small volume and convenient carrying, and can realize the purpose of nucleic acid detection on site.

As shown in fig. 3 and 4, in an embodiment of the present invention, the optical detection module 30 includes an emitting end module (fig. 4 solid line portion) and a reflecting end module (fig. 4 dotted line portion), the emitting end module is used for providing the light source 31 for the nucleic acid detection process, so as to make the designated position of the nucleic acid detection piece emit fluorescence, and the reflecting end module is used for transmitting the fluorescence to the photodetector 38, so as to complete the detection of the total amount of nucleic acid in the nucleic acid detection process. The transmitting end module comprises a light source 31, a first lens 32, a first optical filter 33, a dichroic splitter 34 and a second lens 35 which are sequentially arranged, and the reflecting end module comprises a second lens 35, a dichroic splitter 34, a second optical filter 36, a third lens 37 and a photoelectric detector 38 which are sequentially arranged.

As shown in the schematic light path diagram of the optical detection module 30 shown in fig. 4, for the emission end module, when the specified position of the nucleic acid detection sheet needs to be detected, firstly, the light emitted by the light source 31 is collimated by the first lens 32 to form parallel light, and since the amplification system in the specified position of the nucleic acid detection sheet is excited to emit light with a specific wavelength, the collimated light after being collimated is filtered by the first optical filter 33 to remove light with a wavelength band that cannot make the amplification system emit fluorescence; then, the filtered light is reflected to the direction of the nucleic acid detecting chip by a dichroic filter 34, and finally, the light is focused on the nucleic acid detecting chip by a second lens 35, so that the amplification system in the designated position is excited and radiated to emit fluorescence.

For the reflection end module, after the amplification system is excited to generate fluorescence, the second lens 35 shapes the generated divergent fluorescence into parallel light, and then the parallel light passes through the dichroic splitter 34, at this time, the light in the wavelength band can pass through the dichroic splitter 34, and because some stray light may exist due to environmental factors, a second optical filter 36 is added behind the dichroic splitter 34 to remove the interference light with the wavelength different from the fluorescence generated by the amplification system, and finally the fluorescence passing through the second optical filter 36 is focused to the photodetector 38 through the third lens 37 to complete the detection of the fluorescence signal, so that the amplification condition of the amplification system at the specified position of the nucleic acid detection plate can be obtained.

In an embodiment of the present invention, the light source 31 is preferably an LED, because the LED light source has a small volume and low energy consumption, it is beneficial to integrate the amplification device in the present solution, so that the amplification device is more compact and portable;

since the dichroic sheet 34 can allow light in a certain wavelength band to pass therethrough and reflect light in another wavelength band. For example, a blue dichroic filter 34 may allow light above 520nm to pass through, while light below 480nm is reflected. Therefore, in an embodiment of the present invention, the emission end module optical path and the reflection end module optical path share one dichroic splitter 34, which, on one hand, can play a role of reflecting the light emitted from the light source 31 to the designated position of the nucleic acid detecting piece during emission, and transmitting the fluorescence emitted from the nucleic acid amplification system to the photodetector 38; on the other hand, the dichroic color separation sheet 34 realizes a catadioptric optical path, so that the space occupied by the transmitting end module optical path and the reflecting end module optical path can be smaller, and the development of the small volume of the amplification device is facilitated.

In an embodiment of the present invention, the first lens 32, the second lens 35, and the third lens 37 included in the optical detection module 30 are aspheric short-focus lenses. The shorter the focal length of the lens is, the larger the visual angle is, and the aspheric short-focus lens can have a larger visual angle, so that more light can be shaped or converged by the lens. More importantly, because the background of the design of the amplification instrument is directed to field use, the device is required to have the characteristics of small volume, good compactness and the like so as to be convenient to carry to the detection field. In addition, in this design, the aspheric short-focus lens is adopted, so that the light can be shaped and focused in a short focal length range, which means that the distance between the optical detection module 30 and the nucleic acid detection chip 10 is very short, so that the structure of the amplification instrument is more compact, the volume is smaller, and the sample detection can be carried to the field more conveniently.

In an embodiment of the present invention, the optical detection module 30 shown in fig. 4 can be expanded to use a plurality of optical detection modules 30 in parallel, that is, the motor driving module 40 drives a plurality of optical detection modules 30 simultaneously to perform amplification detection on the nucleic acid detecting piece 10. For example, when the fluorescence generated by the excitation of the amplification system a is red, the fluorescence generated by the excitation of the amplification system B is blue, and the amplification systems a and B need to be detected simultaneously, the optical detection modules 30 can be expanded, so that the two optical detection modules 30 configured with different color light sources are driven by the driving module 40 simultaneously to complete the detection. The expansion of the optical detection module 30 is not limited to the expansion into two optical detection modules 30, and a plurality of optical detection modules 30 may detect in parallel.

As shown in fig. 5, in an embodiment of the present invention, the temperature change module 20 may include: a heat source 21, a heat radiation fin 22, a heat radiator 23 and a heat conductive metal 24, wherein the heat radiation fin 22 is arranged outside the heat source 21, the heat radiator 23 is arranged outside the heat source 21, and the heat conductive metal 24 is arranged between the heat source 21 and the nucleic acid detecting piece 10. Therefore, the heat source 21 and the nucleic acid detecting piece 10 are in close contact, and the heat conducting medium between the heat source 21 and the nucleic acid detecting piece 10 is the heat conducting metal 24 with very small heat resistance, so that the heat conduction efficiency between the heat source 21 and the nucleic acid detecting piece 10 can be ensured, and the temperature change can be rapidly realized to meet the temperature condition of the amplification in the nucleic acid detecting process.

In one embodiment of the present invention, the heat source 21 may include a semiconductor cooling fin. Because the semiconductor refrigeration piece is a sheet-shaped and small-volume electronic element and the nucleic acid detection piece 10 is also of a sheet-shaped structure, the size of the semiconductor refrigeration piece can be selected according to the size of the nucleic acid detection piece 10, so that the heat of the heat source 21 can be more effectively transferred to the nucleic acid detection piece 10, and the problem that the volume of the whole amplification device is large due to the fact that the selected semiconductor refrigeration piece is too large can be solved. In addition, the semiconductor refrigeration piece is an electronic element which can heat and refrigerate, the heating and refrigerating efficiency is very high, most of the semiconductor refrigeration pieces can reach the maximum temperature difference within less than one minute, and therefore the semiconductor refrigeration piece is adopted as the heat source 21, and the temperature change environment required by the nucleic acid detection process can be better met.

As shown in fig. 6 to 12, a nucleic acid detecting sheet 10 according to an embodiment of the present invention includes: test strip body 1, first closure 2 and apron 4, wherein:

the detection piece body 1 and the first closing body 2 are both of a sheet structure, the thermal resistance can be effectively reduced through the type selection of the closing body 2, and the heat exchange efficiency is improved, namely the thickness of the first closing body 2 is smaller, so that the heat exchange efficiency of the detection piece in the temperature changing process can be improved, the heating or cooling speed of the nucleic acid detection piece 10 is higher, the temperature is more balanced, and the time of the nucleic acid detection process is favorably shortened.

The first side of the detection piece body 1 is provided with at least one injection hole 11 and at least one vent hole 12, the second side of the detection piece body 1 is provided with at least one first flow channel groove 13, at least one second flow channel groove 14 and at least one groove 15, and the first closing body 2 is arranged on the second side of the detection piece body 1 so as to form at least one first flow channel, at least one second flow channel and at least one chamber (not shown in the figure) by surrounding with the detection piece body 1, wherein the at least one chamber is used for placing a nucleic acid detection reagent. That is, the second side of the test strip body 1 is a semi-open structure, and at least one first channel groove 13, at least one second channel groove 14 and at least one groove 15 are partially exposed on the outer surface, so as to facilitate injecting different reagents into different grooves 15. Moreover, the nucleic acid detection reagent is placed in the detection sheet in advance, so that the possibility of reagent pollution can be greatly reduced, the detection accuracy is improved, and the preparation operation of the detection reagent is simplified.

In the prior art, the reagent is high in requirement on a preservation mode under low-temperature and dry conditions, the operation is inconvenient, the reagent is exposed in the air for a long time and is easy to volatilize, oxidize, change in pH value and the like, and thus the accuracy of nucleic acid detection is reduced. Therefore, the embodiment of the utility model provides a can pour into different reagent into different recess 15 back in advance, carry out dry low temperature to these reagents and handle, make it form powdered and can adhere to on recess 15's inner wall, nucleic acid detection piece 10 after drying process so can be at room temperature, sealed, long-term storage under the dry condition, also can avoid the volatile oxidation etc. of reagent simultaneously, guaranteed nucleic acid detection's accuracy.

The at least one sample injection hole 11, the at least one first flow channel, the at least one chamber, the at least one second flow channel and the at least one vent hole 12 are sequentially communicated, and a sample to be detected can enter the at least one chamber through the at least one sample injection hole 11 and the at least one first flow channel to react with a nucleic acid detection reagent. After the reagent is dried and processed at low temperature, the first closing body 2 is disposed at the second side of the detection sheet body 1 to provide a place for the reaction between the sample to be detected and the reagent, that is, at least one first flow channel, at least one second flow channel and at least one chamber are formed by surrounding the detection sheet body 1. Moreover, the reagent kit in the prior art usually needs at least 20-50ul of reaction system, and the nucleic acid detecting piece 10 provided by the embodiment of the utility model can complete the reaction only needing about 5-10ul of reaction system, thereby being beneficial to saving the reagent dosage.

The second side of the cover plate 4 is fixed with the first side of the detection piece body 1 to cover all the sample injection holes 11 and all the exhaust holes 12, so that the sample to be detected can be prevented from overflowing from the sample injection holes 11 or the exhaust holes 12, and the volatilization of the reagent and the sample to be detected in the temperature rise and fall reaction process can be prevented.

In some embodiments, the first closing body 2 can be disposed on the second side of the detection sheet body 1 by heat sealing, so that the first closing body 2 can be attached to the detection sheet body 1 without air bubbles, thereby ensuring independent closing of the first flow channel groove 13, the second flow channel groove 14 and the groove 15.

In some embodiments, the first closing body 2 is made of a plastic material, so that the first closing body 2 can have certain heat resistance. For example, the plastic material may be PP, PC, PS, COC, PMMA, etc., and is not particularly limited herein.

In some embodiments, the thickness of the first sealing member 2 is 0.01mm to 0.2mm in a direction from the first side of the test strip body 1 to the second side of the test strip body 1, since the nucleic acid test strip 10 needs to be heated by the test device after the nucleic acid test strip 10 is inserted into the test device, in this embodiment, the first sealing member 2 is heated at a side away from the test strip body 1. Through the material of selecting first obturator 2 and the thickness of optimizing first obturator 2, can be favorable to improving the heat transfer efficiency of nucleic acid detecting piece 10 heat transfer process, improve the speed of rising and falling the temperature of reaction to shorten the check-out time.

The detection piece body 1 in the embodiment shown in fig. 7 and 8 has sixteen injection holes 11, sixteen first channel grooves 13, sixteen grooves 15, sixteen second channel grooves 14 and sixteen vent holes 12, each injection hole 11 is communicated with one of the first channel grooves 13, one of the grooves 15, one of the second channel grooves 14 and one of the vent holes 12, that is, the nucleic acid detection piece 10 can pre-seal sixteen different reagents and detect sixteen samples to be detected; the detection chip body 1 in the embodiment shown in fig. 9 and 10 has one injection hole 11, twenty first channel grooves 13, twenty grooves 15, twenty second channel grooves 14 and twenty vent holes 12, and the one injection hole 11 is respectively communicated with the twenty first channel grooves 13, the twenty grooves 15, the twenty second channel grooves 14 and the twenty vent holes 12, that is, the nucleic acid detection chip 10 can pre-encapsulate twenty reagents to realize twenty-target detection on a sample; the detection piece body 1 in the embodiment shown in fig. 11 and 12 has four injection holes 11, sixteen first channel grooves 13, sixteen grooves 15, sixteen second channel grooves 14, and sixteen vent holes 12, each injection hole 11 is communicated with four of the first channel grooves 13, four of the grooves 15, four of the second channel grooves 14, and four of the vent holes 12, that is, the nucleic acid detection piece 10 can pre-encapsulate four different reagents to realize nucleic acid detection on 4 samples to be detected, and each sample to be detected can simultaneously detect at least four targets.

The nucleic acid detection sheet 10 provided by the above three embodiments can respectively complete the detection of 16 samples with a maximum of 4 targets in a single chamber, a maximum of 80 targets in a single sample 20 chamber, and a maximum of 16 targets in a 4 sample 4 chamber. When the multi-target detection task is carried out, an operator only needs to inject the sample to be detected into the sample injection hole 11 for one time, and the multi-target detection task can be completed, so that the pipetting operation of the sample to be detected can be greatly reduced, the possibility of reagent pollution in the operation process can be reduced, and the workload of the operator is reduced. Moreover, the nucleic acid detecting sheet 10 can be prepared in advance according to actual detection requirements, thereby realizing a customized, quantitative, more flexible and convenient nucleic acid detection mode.

It can be known that the detecting piece body 1 provided by the above three embodiments is only three examples performed by the embodiments of the present invention, and of course, other detecting piece bodies 1 belonging to the concept of the present invention are also within the protection scope of the present invention.

In some embodiments, the detection sheet body 1 may be made of a transparent plastic material, and is manufactured by an injection molding process, which is beneficial to improving the processing efficiency of the detection sheet body 1. For example, the plastic material may be PP, PC, PS, COC, PMMA, etc., and is not particularly limited herein. The selection of the material can ensure that the detection piece body 1 has certain heat resistance and good light transmittance, and can detect the fluorescent signal in the reaction process. The part of the detection piece body 1 far away from the sample injection hole 11 and the exhaust hole 12 can be provided with a fishtail-shaped handle to facilitate the taking and placing of an operator, and meanwhile, the part can be provided with a plurality of bulges to further facilitate the taking and placing of the operator. Of course, a two-dimensional code can be pasted on a proper position of the detection sheet body 1, and the two-dimensional code can simply and quickly identify the relevant information of the corresponding nucleic acid detection sheet 10, such as information of chip specification, reagent type, production batch number, effective date, detection flow, calibration data, and the like.

In some embodiments, at least one of the grooves 15 may be arranged in an array structure according to a predetermined rule for optical detection, for example, the predetermined rule may be parallel to each other, and the length direction of each groove 15 may be perpendicular to or have an angle with the arrangement direction of the exhaust holes 12, and the arrangement direction of the grooves 15 is not particularly limited.

In one embodiment of the present invention, the nucleic acid detecting sheet 10 further includes: a second enclosure 3;

the second sealing member 3 is disposed on the first side of the test strip body 1 to cover all the exhaust holes 12.

In the embodiment of the present invention, the second closing body 3 can prevent the sample to be detected from possibly flowing out from the air vent 12.

In some embodiments, the second enclosure 3 comprises a waterproof, gas-permeable membrane, i.e. the second enclosure 3 is not only able to prevent the sample to be detected from possibly flowing out through the vent 12, but also to facilitate venting of the gas in the chamber.

It should be noted that the gas is present in the first flow channel, the chamber and the second flow channel, when the sample to be detected is injected into the chamber, the gas, the sample to be detected and the reagent are present in the chamber at the same time, and if the gas is not discharged from the chamber or the detection chip body 1, the nucleic acid detection chip 10 may generate a gas-liquid two-phase flow interaction during the temperature changing process, which may cause the liquid in the chamber to move to other positions, thereby causing the detection device to fail to detect the signal.

In an embodiment of the present invention, the cover plate 4 can also be made of plastic material, so that the cover plate 4 has a certain heat resistance. For example, the plastic material may be PP, PC, PS, COC, PMMA, etc., and is not particularly limited herein.

In one embodiment of the present invention, the nucleic acid detecting sheet 10 further includes: a soft rubber cushion 5;

the cover plate 4 is fixed to the first side of the soft rubber pad 5, and the second side of the soft rubber pad 5 is fixed to the first side of the test piece body 1 so as to cover all the sample injection holes 11 and all the exhaust holes 12.

The embodiment of the utility model provides an in, soft cushion 5 is fixed and is being lapped 4 and detect between piece body 1, is favorable to increasing apron 4 to annotating the leakproofness of appearance hole 11 or exhaust hole 12, improves ambient pressure under the high temperature condition, is favorable to preventing more to detect that the sample injection process is probably overflowed from annotating appearance hole 11 or exhaust hole 12 to and can prevent reagent and wait to detect the sample and rise volatilizing of temperature rise reaction process. For example, the soft rubber pad 5 may include a double-sided tape, a silicon gel, and the like, and when the soft rubber pad 5 adopts a double-sided tape, it can be ensured that the soft rubber pad 5 is more firmly fixed with the cover plate 4 and the detection sheet body 1, respectively.

In an embodiment of the present invention, the first side of the detecting piece body 1 is provided with an installation groove 16, and the cover plate 4 is fixed in the installation groove 16;

one of the cover plate 4 and the mounting groove 16 is provided with a buckle, and the other is provided with a clamping part, and the buckle and the clamping part are clamped.

The embodiment of the utility model provides an in, be favorable to guaranteeing the effect of acceping to apron 4 through setting up mounting groove 16, secondly utilize the joint cooperation of buckle and joint portion (not shown in the figure), also be favorable to the assembly of apron 4 and mounting groove 16. In addition, all the injection holes 11 and all the exhaust holes 12 are located within the range enclosed by the mounting groove 16, so that the sealing of the two through the cover plate 4 is facilitated.

In an embodiment of the present invention, the first side of the test piece body 1 is further provided with a groove 17, and the groove 17 is disposed inside the mounting groove 16 and disposed at the periphery of all the sample injection holes 11 and all the exhaust holes 12.

The embodiment of the utility model provides an in, through setting up slot 17, even if remain to detect the sample and spill over from annotating appearance hole 11 or exhaust hole 12, also can flow the this slot 17 of passageway earlier, after covering apron 4 or covering apron 4 and soft cushion 5, alright detect the sample and can not spill over again in order to guarantee to detect at the in-process of Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) to can play more reliable sealed effect.

The utility model discloses an embodiment, recess 15 is the structure that both ends expand gradually to the centre, and recess 15 is being advanced a kind and is being provided with the cambered surface with a appearance department promptly, so be favorable to reducing to detect the shape resistance pressure drop that the sample flows into recess 15 and flows into second runner 14 flow process from first runner 13 from recess 15, guarantee flow stability, the steady flow wait to detect the sample can effectively be with the gas outgoing in the recess 15 under capillary force and surface tension's interact.

In an embodiment of the present invention, when one injection hole 11 of the at least one injection hole 11 is respectively communicated with a plurality of exhaust holes 12 of the at least one exhaust hole 12, the at least one first channel groove 13 includes: a main runner groove 131 and a plurality of branch runner grooves 132;

a first end of the main runner channel 131 is communicated with the sample injection hole 11, a second end of the main runner channel 131 is communicated with first ends of the plurality of branch runner channels 132, and second ends of the plurality of branch runner channels 132 are communicated with at least one groove 15;

along the flowing direction of the sample to be detected, the main channel groove 131 and each of the plurality of branch channel grooves 132 are arranged at an obtuse angle.

In the embodiment of the present invention, along the flowing direction of the sample to be detected, the main channel groove 131 and each branch channel groove 132 of the plurality of branch channel grooves 132 are arranged at an obtuse angle, and the obtuse angle can drive the sample to be detected to flow in a fixed direction, which is favorable for the sample to be detected to sequentially enter the nearby chamber or the groove 15; meanwhile, in the injection process or the temperature rise and drop process of the sample to be detected, the sample to be detected in the upstream cavity can be prevented from streaming to the adjacent downstream cavity through the design mode, and therefore the accuracy of the detection process can be further guaranteed. Of course, the main channel groove 131 and each of the plurality of branch channel grooves 132 may be disposed at an acute angle along the flowing direction of the sample to be detected.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising a" does not exclude the presence of other similar elements in a process, method, article, or apparatus that comprises the element.

Finally, it is to be noted that: the above description is only the preferred embodiment of the present invention, which is only used to illustrate the technical solution of the present invention, and is not used to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present invention are all included in the protection scope of the present invention.

Claims (9)

1. An amplification device for use in nucleic acid detection, comprising: a nucleic acid detection sheet (10), a temperature changing module (20), an optical detection module (30), a motor driving module (40) and a shell (50);

the temperature changing module (20), the optical detection module (30) and the motor driving module (40) are accommodated in the housing (50), the housing (50) is provided with a card slot (51), and the nucleic acid detection card (10) can be inserted into the card slot (51);

the temperature-changing module (20) is arranged at the bottom of the shell (50), the nucleic acid detection sheet (10) is arranged above the temperature-changing module (20), the optical detection module (30) is arranged above the nucleic acid detection sheet (10), and the motor driving module (40) is arranged above the optical detection module (30);

the nucleic acid detection sheet (10) is used for placing a nucleic acid reagent and a sample to be detected;

the temperature changing module (20) is used for providing a temperature environment required by the reaction according to the condition of the reaction between the nucleic acid reagent and the sample to be detected;

the optical detection module (30) is used for providing a detection light source (31) for the nucleic acid detection process and detecting fluorescence emitted by different positions of the nucleic acid detection piece (10);

the motor driving module (40) is used for driving the optical detection module (30) to move so as to detect different positions in the nucleic acid detection piece (10).

2. The amplification apparatus for nucleic acid detection according to claim 1, wherein the optical detection module (30) comprises: a light source (31), a first lens (32), a first filter (33), a dichroic filter (34), a second lens (35), a second filter (36), a third lens (37), and a photodetector (38);

the first lens (32) is used for converting the light emitted by the light source (31) into parallel light;

the first optical filter (33) is used for filtering the light which cannot enable the sample to be detected to generate fluorescence in the parallel light after passing through the first lens (32);

the dichroic sheet (34) for changing an optical path of the parallel light after passing through the first filter (33) to reflect the optical path to the nucleic acid detecting sheet (10), and for transmitting the fluorescence passing through the second lens (35) to the photodetector (38);

the second lens (35) is used for focusing the light reflected by the dichroic chip (34) on the nucleic acid detection chip (10) and converting the fluorescence emitted by the nucleic acid detection chip (10) into parallel light;

the second optical filter (36) is used for removing interference light except the fluorescence;

the third lens (37) is used for focusing the light passing through the second optical filter (36) onto the photoelectric detector (38);

the light source (31), the first lens (32), the first filter (33), the dichroic filter (34), and the second lens (35) are sequentially disposed along an optical path of the light source (31) to irradiate light emitted from the light source (31) onto the nucleic acid detecting sheet (10);

the second lens (35), the dichroic filter (34), the second filter (36), the third lens (37), and the photodetector (38) are sequentially disposed along an optical path of the fluorescence, so that the fluorescence is detected by the photodetector (38).

3. The amplification apparatus for nucleic acid detection according to claim 2, wherein the temperature-varying module (20) comprises: a heat source (21), heat-radiating fins (22), a heat sink (23), and a heat-conductive metal (24);

the heat source (21) is used for providing a temperature environment required by the reaction for the nucleic acid detection process;

the heat radiating fins (22) are arranged outside the heat source (21), the heat radiator (23) is arranged outside the heat source (21), and the heat conducting metal (24) is arranged between the heat source (21) and the nucleic acid detecting piece (10);

and/or the presence of a gas in the gas,

the first lens (32), the second lens (35), and the third lens (37) are all aspheric short-focus lenses.

4. The amplification apparatus for nucleic acid detection according to any one of claims 1 to 3, wherein the nucleic acid detection sheet (10) comprises: the detection piece comprises a detection piece body (1), a first closing body (2) and a cover plate (4);

the detection sheet body (1) and the first closing body (2) are both sheet-shaped structures;

the first side of the detection sheet body (1) is provided with at least one sample injection hole (11) and at least one exhaust hole (12), the second side of the detection sheet body (1) is provided with at least one first flow channel groove (13), at least one second flow channel groove (14) and at least one groove (15), and the first closing body (2) is arranged on the second side of the detection sheet body (1) so as to surround the detection sheet body (1) to form at least one first flow channel, at least one second flow channel and at least one cavity;

the at least one sample injection hole (11), the at least one first flow channel, the at least one chamber, the at least one second flow channel and the at least one vent hole (12) are communicated in sequence;

the at least one chamber is used for placing nucleic acid detection reagents;

the sample to be detected can enter the at least one chamber through the at least one injection hole (11) and the at least one first flow channel to react with the nucleic acid detection reagent;

the cover plate (4) is fixed with the first side of the detection sheet body (1) to cover all the sample injection holes (11) and all the exhaust holes (12).

5. The amplification apparatus for nucleic acid detection according to claim 4, wherein the nucleic acid detection sheet (10) further comprises: a second enclosure (3);

the second closing body (3) is arranged on the first side of the detection sheet body (1) to cover all the exhaust holes (12);

the second closure (3) comprises a waterproof breathable membrane;

and/or the presence of a gas in the gas,

the nucleic acid detecting sheet (10) further comprises: a soft rubber cushion (5);

the cover plate (4) is fixed with the first side of the soft rubber cushion (5), the second side of the soft rubber cushion (5) is fixed with the first side of the detection sheet body (1) to cover all the sample injection holes (11) and all the exhaust holes (12).

6. The amplification apparatus for nucleic acid detection according to claim 4, wherein the detection piece body (1) has sixteen of the injection holes (11), sixteen of the first channel grooves (13), sixteen of the grooves (15), sixteen of the second channel grooves (14), and sixteen of the exhaust holes (12), each of the injection holes (11) communicating with one of the first channel grooves (13), one of the grooves (15), one of the second channel grooves (14), and one of the exhaust holes (12);

or the like, or, alternatively,

the detection sheet body (1) is provided with one sample injection hole (11), twenty first flow channel grooves (13), twenty grooves (15), twenty second flow channel grooves (14) and twenty exhaust holes (12), and the sample injection hole (11) is respectively communicated with the twenty first flow channel grooves (13), the twenty grooves (15), the twenty second flow channel grooves (14) and the twenty exhaust holes (12);

or the like, or, alternatively,

the detection piece body (1) is provided with four sample injection holes (11), sixteen first flow channel grooves (13), sixteen grooves (15), sixteen second flow channel grooves (14) and sixteen exhaust holes (12), and each sample injection hole (11) is communicated with four of the first flow channel grooves (13), four of the grooves (15), four of the second flow channel grooves (14) and four of the exhaust holes (12).

7. The amplification apparatus for nucleic acid detection according to claim 4, wherein the first side of the detection piece body (1) is provided with a mounting groove (16), and the cover plate (4) is fixed in the mounting groove (16);

apron (4) with one in mounting groove (16) is provided with the buckle, and another is provided with joint portion, the buckle with joint portion joint.

8. The amplification apparatus for nucleic acid detection according to claim 7, wherein the first side of the detection piece body (1) is further provided with a groove (17), and the groove (17) is provided inside the mounting groove (16) and on the outer periphery of all the injection holes (11) and all the exhaust holes (12).

9. The amplification apparatus for nucleic acid detection according to claim 4, wherein the detection piece body (1), the first closing body (2) and the cover plate (4) are made of plastic material;

and/or the presence of a gas in the gas,

the thickness of the first closing body (2) along the direction from the first side of the detection sheet body (1) to the second side of the detection sheet body (1) is 0.01mm-0.2 mm;

and/or the presence of a gas in the gas,

the groove (15) is a structure with two ends gradually expanding towards the middle.

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