CN107505266B - Semi-automatic optical detection analysis system - Google Patents
Semi-automatic optical detection analysis system Download PDFInfo
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- CN107505266B CN107505266B CN201710690337.3A CN201710690337A CN107505266B CN 107505266 B CN107505266 B CN 107505266B CN 201710690337 A CN201710690337 A CN 201710690337A CN 107505266 B CN107505266 B CN 107505266B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The invention introduces a semi-automatic optical detection analysis system, which comprises an optical detection system and a control system, wherein the optical detection system is composed of an LED driving circuit module, an LED light source, a coupling optical fiber module, a sample detection module and a signal regulation circuit module, and the control system is composed of a data acquisition circuit module, a main control circuit module and an LCD display screen; the input end of the LED driving circuit module is connected with the output interface of the main control circuit module, the output end of the LED driving circuit module is connected with the power input end of the LED light source, a coupling optical fiber module is arranged on one side close to the LED light source, the output end of the coupling optical fiber module is connected with the input end of the sample detection module, the output end of the sample detection module is connected with the input end of the signal regulation circuit module through optical communication, and the I/O interface on the signal regulation circuit module is in bidirectional communication connection with one I/O interface of the data acquisition circuit module. The system of the invention has compact and simple structure and convenient assembly, and is very suitable for miniaturization.
Description
Technical Field
The invention relates to a biochemical analysis and detection system, in particular to a semiautomatic optical detection and analysis system, and belongs to the technical field of optical detection equipment.
Background
The measurement principle of the semi-automatic multi-parameter biochemical analyzer is based on a spectrophotometry, after a color reaction is carried out in a detection chamber, the characteristic spectrum light intensity after the absorption of a substance is measured, and the absorbance is obtained by utilizing the lambert beer law, so that the concentration of the detected substance is obtained. The multi-parameter semi-automatic biochemical analyzer based on spectrophotometry has the characteristics of portability, rapidness, low cost and intellectualization, can perform biochemical multi-parameter concentration measurement, and has become the most widely applied instrument in the biochemical parameter measurement industry at present.
At present, a domestic semiautomatic biochemical analyzer mainly adopts a halogen lamp as a light source, the light source adopts a rotary optical filter mode to obtain monochromatic light with multiple wavelengths, and a silicon photodiode is adopted to receive signals, so that the detection of the wavelength range of 340nm-850nm is realized. The U.S. RT-9000 type semiautomatic biochemical analyzer also uses a rotating filter disk for the halogen lamp light source to obtain multiple wavelength beams. The ECOM-F6124 type semiautomatic biochemical analyzer in Germany is internally provided with 7 standard color filters to carry out light splitting on the spectrum absorbed by the sample chamber, and can realize detection of seven wavelengths within the range of 340nm-690 nm. Recently, a 2-generation miniature rapid biochemical analyzer is developed by a microsystem center of Chongqing university, a first generation adopts monochromatic LEDs with different wavelengths as light sources, a plurality of sample chambers are provided, a miniature optical fiber spectrometer is adopted to receive signals, and the signal acquisition work is completed by moving a probe of the optical fiber spectrometer to a detection port of each sample chamber; in the second generation, a halogen lamp is used as a light source, a micro optical fiber spectrometer is used as a receiver, and a plurality of sample chambers are still arranged so as to move the sample chambers into a light path to complete the acquisition of photoelectric signals. At present, a great deal of foreign literature is studied to realize biochemical multi-parameter multi-index detection based on a micro spectrometer and a sequential injection method.
The analysis of the current situation of domestic and foreign researches shows that the semi-automatic multi-parameter biochemical analyzer generally adopts the following scheme for realizing multi-parameter detection: firstly, light sources such as optical filters and monochromatic LED light sources are split; and secondly, the photoelectric receiver is subjected to light splitting, such as a micro spectrometer. The analyzer can detect biochemical multiple parameters and is accurate in measurement. However, the existing optical detection and analysis system is not compact, has large miniaturization difficulty and has high cost; the most critical is that the optical systems of the semi-automatic multi-parameter biochemical analyzers all have movable mechanical parts, which inevitably brings certain system errors; the movable part is easy to have mechanical failure or initial position error and the like after long-time use, and is not easy to control; the introduction of moving parts necessarily reduces the accuracy and repeatability of the instrument measurements.
Disclosure of Invention
Aiming at the defects in the prior art, the invention mainly aims to solve the problems that the existing optical detection and analysis system is complex in structure, difficult to miniaturize and high in cost, and provides a semi-automatic optical detection and analysis system which can perform light-splitting treatment and has no movable mechanical component and has the advantages of simple and compact structure, small volume, rapid detection, simple operation, low cost and the like.
The technical scheme of the invention is as follows: the semi-automatic optical detection analysis system is characterized by comprising an optical detection system and a control system, wherein the optical detection system is composed of an LED driving circuit module, an LED light source, a coupling optical fiber module, a sample detection module and a signal regulation circuit module, and the control system is composed of a data acquisition circuit module, a main control circuit module and an LCD display screen; the LED display device comprises a main control circuit module, an LED driving circuit module, a signal adjusting circuit module, a data acquisition circuit module, a coupling optical fiber module, a sample detection module and an LCD display screen, wherein the input end of the LED driving circuit module is connected with the output interface of the main control circuit module; the sample detection module comprises an aluminum alloy shell, a transparent sample solution box is arranged in the aluminum alloy shell, the lower part of the sample solution box is arranged in the aluminum alloy shell, the upper part of the sample solution box extends out of the aluminum alloy shell, and distilled water is filled between the aluminum alloy shell and the sample solution box; a left sleeve is arranged on the left side of the aluminum alloy shell, a right sleeve is arranged on the right side of the aluminum alloy shell, the left sleeve and the right sleeve are transversely embedded in the aluminum alloy shell, an outer opening of the left sleeve and an outer opening of the right sleeve are respectively and hermetically connected with the aluminum alloy shell, and the inner side of the left sleeve and the inner side of the right sleeve are tightly adhered to and hermetically connected with the sample solution box; a left lens is arranged at the outer opening of the left sleeve, a right lens is arranged at the outer opening of the right sleeve, and the left lens and the right lens are positioned on the same horizontal plane and are opposite to each other; a heating pipe is arranged in an aluminum alloy shell at the bottom of the sample solution box, the input end of the heating pipe is connected with the output end of a temperature controller arranged at the outer side of the aluminum alloy shell through a wire, the input end of the temperature controller is electrically connected with a thermocouple temperature sensor arranged on the aluminum alloy shell, and a probe of the thermocouple temperature sensor is arranged in the aluminum alloy shell; the stirring device is characterized in that a stirring motor is further arranged below the aluminum alloy shell, an output shaft of the stirring motor extends upwards and is fixedly connected with a magnet tray, an S-pole magnet and an N-pole magnet are arranged on the magnet tray, a rotatable stirring magnet is arranged at the bottom of the inner side of the sample solution box, and the stirring magnet is matched with the S-pole magnet and the N-pole magnet.
Optimally, the LED light source consists of at least three monochromatic LED lamps, and the monochromatic LED lamps are respectively provided with independent power supply control switches; the coupling optical fiber module consists of at least three optical fiber input joints and one optical fiber output joint, each optical fiber input joint corresponds to a single-color LED lamp respectively, and the optical fiber output joint is connected with the input end of the sample detection module. The invention adopts a plurality of groups of monochromatic LED lamps to be used simultaneously, when in use, the corresponding power control switch is used for controlling a group of monochromatic LED lamps to be lightened, and then the light source is transmitted to the sample detection module through the corresponding optical fiber input connector.
Optimally, a cuvette is also arranged at the top of the sample solution box. The invention can improve the detection precision by installing the cuvette, and is convenient to use and maintain.
Optimally, the cross section of the left sleeve and the cross section of the right sleeve are elliptical, the left sleeve is matched with the left lens, and the right sleeve is matched with the right lens; the stirring magnet is opposite to the output shaft of the stirring motor, and the S-pole magnet and the N-pole magnet are symmetrically arranged relative to the output shaft of the stirring motor. The light source signals are accurately transmitted through the mutually symmetrical lenses, so that the purpose of reducing errors is achieved. Meanwhile, the stirring of the sample solution is realized through the stirring magnet, the stirring magnet is driven to rotate through the rotation of the magnet, and the motor does not need to be penetrated into the sample solution box, so that the tightness and the detection precision of the equipment are ensured, and the constant temperature is kept.
Optimally, the LED driving circuit module comprises a power input end V1, wherein the power input end V1 is respectively connected with one end of a resistor R1, one end of a resistor R2 and one end of a resistor R3, and the other end of the resistor R1 is connected with a diode D2 in the forward direction and then connected to the cathode of a light-emitting diode D4; the other end of the resistor R2 is connected with the cathode of the light emitting diode D3, the other end of the resistor R3 is connected with the diode D1 in the forward direction and then is respectively connected with one end of the capacitor C1, the power output end V2, the anode of the light emitting diode D3, the anode of the light emitting diode D4 and the anode of the light emitting diode D5, the other end of the capacitor C1 is grounded, and the cathode of the light emitting diode D5 is connected with the resistor R4 and then is grounded.
Optimally, the signal conditioning circuit module comprises a power input end V3, wherein the power input end V3 is respectively connected with one end of a capacitor C2, a source electrode of a field effect tube D8, a source electrode of a field effect tube D7, a cathode of a photodiode D6 and one end of a resistor R5, the other end of the resistor R5 is respectively connected with an anode of the photodiode D6, a grid electrode of the field effect tube D7 and one end of an inductor L, the other end of the inductor L is respectively connected with one end of the inductor L2 and one end of the resistor R6, the other end of the inductor L2 is respectively connected with a drain electrode of the field effect tube D7 and a grid electrode of the field effect tube D8, and the other end of the resistor R6 is respectively connected with a drain electrode of the field effect tube D8, the other end of the capacitor C2 and a bidirectional communication interface U1.
Optimally, the data acquisition circuit module comprises a chip IC with the model of ADC0809CCN, a first pin of the chip IC is respectively connected with a fifth pin of the chip IC, one end of a resistor R12, one end of a resistor R10 and a negative electrode of a diode D9, an anode of the diode D9 is respectively connected with one end of a bidirectional communication interface U2 and one end of a resistor R8 after being connected with a resistor R7, the other end of the resistor R8 is respectively connected with one end of a capacitor C3 and one end of the resistor R9, the other end of the capacitor C3 is respectively connected with the other end of the resistor R9, one end of a capacitor C4, one end of a resistor R11 and one end of a capacitor C5 and is grounded, the other end of the capacitor C4 is respectively connected with the other end of the resistor R11 and the other end of the resistor R10, a third pin of the chip IC is grounded, and a seventh pin of the chip IC is respectively connected with the other end of the resistor R12 and one end of the resistor R13, and the other end of the resistor R13 is connected with the bidirectional communication interface U3.
In the invention, the specific LED driving circuit module, the signal adjusting circuit module and the data acquisition circuit module are adopted, so that the technical scheme of the invention is complete and can be realized, and meanwhile, the adopted circuit module structure with specific design has better reliability compared with the prior art, is beneficial to the design of small models, has lower energy consumption, is convenient for improving the detection precision, and obviously enhances the stability during working.
Compared with the prior art, the invention has the following beneficial effects:
1. the system has compact and simple structure and convenient assembly, and is very suitable for the design of miniaturized, portable and even hand-held semi-automatic biochemical analyzers.
2. Due to the structural variability, the method is very suitable for developing single-parameter to any multi-parameter serial products, and greatly shortens the development time and saves the development cost of the die.
3. The optical system adopts all fixed parts, has no movable part, greatly reduces the system error brought by the optical system, and ensures the measurement accuracy and repeatability of the whole instrument.
4. The novel multi-parameter biochemical analyzer optical system structure has the core idea that the light source is split by utilizing the multi-input and one-output coupling optical fiber, the optical system has certain universality, and the system can be used for constructing a semi-automatic biochemical analyzer from single parameters to any multi-parameters only by changing the number of front-end light sources.
Drawings
FIG. 1 is a schematic diagram of a semi-automatic optical detection and analysis system according to the present invention.
Fig. 2 is a schematic circuit diagram of an LED driving circuit module according to the present invention.
Fig. 3 is a schematic circuit diagram of a signal conditioning circuit module according to the present invention.
Fig. 4 is a schematic circuit diagram of a data acquisition circuit module according to the present invention.
FIG. 5 is a schematic diagram of a sample detection module according to the present invention.
In the figure, a 1-LED driving circuit module, a 2-LED light source, a 21-monochromatic LED lamp, a 3-coupling optical fiber module, a 31-optical fiber input connector, a 32-optical fiber output connector, a 4-sample detection module, a 5-signal adjusting circuit module, a 6-data acquisition circuit module, a 7-main control circuit module, an 8-LCD display screen, a 41-aluminum alloy shell, a 42-sample solution box, a 43-left sleeve, a 44-right sleeve, a 45-left lens, a 46-right lens, a 47-heating pipe, a 48-temperature controller, a 49-thermocouple temperature sensor, a 410-stirring motor, a 411-magnet tray, a 412-S pole magnet, a 413-N pole magnet, a 414-stirring magnet and a 415-cuvette.
Detailed Description
The invention is further described below with reference to the drawings and the detailed description.
As shown in fig. 1, 2, 3, 4 and 5, the semiautomatic optical detection and analysis system of the invention comprises an optical detection system and a control system, wherein the optical detection system is composed of an LED driving circuit module 1, an LED light source 2, a coupling optical fiber module 3, a sample detection module 4 and a signal adjustment circuit module 5, and the control system is composed of a data acquisition circuit module 6, a main control circuit module 7 and an LCD display screen 8; the input end of the LED driving circuit module 1 is connected with the output interface of the main control circuit module 7, the output end of the LED driving circuit module 1 is connected with the power input end of the LED light source 2, one side close to the LED light source 2 is provided with a coupling optical fiber module 3, the input end of the coupling optical fiber module 3 is opposite to the LED light source 2, the output end of the coupling optical fiber module 3 is connected with the input end of the sample detection module 4 for detecting light source signals, the output end of the sample detection module 4 is connected with the input end of the signal regulation circuit module 5 through optical communication, the I/O interface on the signal regulation circuit module 5 is in bidirectional communication connection with one I/O interface of the data acquisition circuit module 6, the other I/O interface of the data acquisition circuit module 6 is in bidirectional communication connection with one I/O interface of the main control circuit module 7, and the other I/O interface of the main control circuit module 7 is in bidirectional communication connection with the LCD display screen 8; the sample detection module 4 comprises an aluminum alloy shell 41, a transparent sample solution box 42 is arranged in the aluminum alloy shell 41, the lower part of the sample solution box 42 is arranged in the aluminum alloy shell 41, the upper part of the sample solution box 42 extends out of the aluminum alloy shell 41, and distilled water is filled between the aluminum alloy shell 41 and the sample solution box 42; a left sleeve 43 is arranged on the left side of the aluminum alloy shell 41, a right sleeve 43 is arranged on the right side of the aluminum alloy shell 41, the left sleeve 43 and the right sleeve 44 are transversely embedded in the aluminum alloy shell 41, the outer opening of the left sleeve 43 and the outer opening of the right sleeve 44 are respectively in sealing connection with the aluminum alloy shell 41, and the inner side of the left sleeve 43 and the inner side of the right sleeve 44 are tightly adhered to and in sealing connection with the sample solution box 42; a left lens 45 is arranged at the outer opening of the left sleeve 43, a right lens 46 is arranged at the outer opening of the right sleeve 44, and the left lens 45 and the right lens 46 are positioned on the same horizontal plane and are opposite to each other; a heating pipe 47 is arranged in the aluminum alloy shell 41 at the bottom of the sample solution box 42, the input end of the heating pipe 47 is connected with the output end of a temperature controller 48 arranged at the outer side of the aluminum alloy shell 41 through a wire, the input end of the temperature controller 48 is electrically connected with a thermocouple temperature sensor 49 arranged on the aluminum alloy shell 41, and the probe of the thermocouple temperature sensor 49 is arranged in the aluminum alloy shell 41; the lower part of the aluminum alloy shell 41 is also provided with a stirring motor 410, an output shaft of the stirring motor 410 extends upwards and is fixedly connected with a magnet tray 411, an S-pole magnet 412 and an N-pole magnet 413 are arranged on the magnet tray 411, a rotatable stirring magnet 414 is arranged at the bottom of the inner side of the sample solution box 42, and the stirring magnet 414 is matched with the S-pole magnet 412 and the N-pole magnet 413.
In the invention, the main control circuit module 7 and the LCD display screen 8 both adopt the existing modules and devices, the main control circuit module 7 is used for controlling the work of each module, and the LCD display screen 8 can display information and send operation instructions to the main control circuit module 7.
In the invention, the temperature controller 8 and the thermocouple temperature sensor 9 are all existing electronic equipment, and the temperature controller 8 is used for receiving temperature data acquired by the thermocouple temperature sensor 9 and then controlling the heating pipe 7 to be opened or closed after comparison, so that the constant temperature control of the sample solution is realized. In the present invention, the stirring motor 10 may be manually controlled or automatically controlled, and the stirring magnet 14 may be controlled to rotate by the stirring motor 10, thereby stirring the sample solution.
Referring to fig. 1, the LED light source 2 is composed of at least three single-color LED lamps 21, and the single-color LED lamps 21 are respectively provided with independent power control switches; the coupling fiber module 3 is composed of at least three fiber input connectors 31 and one fiber output connector 32, each fiber input connector 31 corresponds to a single-color LED lamp 21, and the fiber output connector 32 is connected with the input end of the sample detection module 4.
Referring to fig. 5, a cuvette 415 is also mounted at the top of the sample solution tank 42. The cross section of the left sleeve 43 and the cross section of the right sleeve 44 are elliptical, the left sleeve 43 is matched with the left lens 45, and the right sleeve 44 is matched with the right lens 46; the stirring magnet 414 is opposite to the output shaft of the stirring motor 410, and the S-pole magnet 412 and the N-pole magnet 413 are symmetrically arranged with respect to the output shaft of the stirring motor 410.
Referring to fig. 2, 3 and 4, the LED driving circuit module 1 includes a power input terminal V1, where the power input terminal V1 is connected to one end of a resistor R1, one end of a resistor R2 and one end of a resistor R3, and the other end of the resistor R1 is connected to a diode D2 in a forward direction and then connected to a cathode of a light emitting diode D4; the other end of the resistor R2 is connected with the cathode of the light emitting diode D3, the other end of the resistor R3 is connected with the diode D1 in the forward direction and then is respectively connected with one end of the capacitor C1, the power output end V2, the anode of the light emitting diode D3, the anode of the light emitting diode D4 and the anode of the light emitting diode D5, the other end of the capacitor C1 is grounded, and the cathode of the light emitting diode D5 is connected with the resistor R4 and then is grounded. The signal conditioning circuit module 5 comprises a power input end V3, wherein the power input end V3 is respectively connected with one end of a capacitor C2, a source electrode of a field effect tube D8, a source electrode of a field effect tube D7, a cathode of a photodiode D6 and one end of a resistor R5, the other end of the resistor R5 is respectively connected with an anode of the photodiode D6, a grid electrode of the field effect tube D7 and one end of an inductor L, the other end of the inductor L is respectively connected with one end of the inductor L2 and one end of the resistor R6, the other end of the inductor L2 is respectively connected with a drain electrode of the field effect tube D7 and a grid electrode of the field effect tube D8, and the other end of the resistor R6 is respectively connected with the drain electrode of the field effect tube D8, the other end of the capacitor C2 and a bidirectional communication interface U1. The data acquisition circuit module 6 comprises a chip IC (integrated circuit) of which the model is ADC0809CCN, a first pin of the chip IC is respectively connected with a fifth pin of the chip IC, one end of a resistor R12, one end of a resistor R10 and a negative electrode of a diode D9, an anode of the diode D9 is respectively connected with one end of a bidirectional communication interface U2 and one end of a resistor R8 after being connected with a resistor R7, the other end of the resistor R8 is respectively connected with one end of a capacitor C3 and one end of the resistor R9, the other end of the capacitor C3 is respectively connected with one end of a capacitor C4, one end of a resistor R11 and one end of a capacitor C5 in a grounding manner, the other end of the capacitor C4 is respectively connected with the other end of the resistor R11 and the other end of the resistor R10, the other end of the capacitor C5 is connected to a fourth pin of the chip IC, the seventh pin of the chip IC is respectively connected with the other end of the resistor R12 and one end of the resistor R13, and the other end of the resistor R13 is connected with the bidirectional communication interface U3.
Working principle: when the automatic optical detection analysis system works, the main control circuit module 7 starts the LED driving circuit module 1, the LED driving circuit module 1 only controls a single-color LED lamp to be lightened at the same time, a single-wavelength light source emitted by the single-color LED lamp 21 passes through the multi-in-one-out coupling optical fiber module 3 and the sample detection module 4, is coupled to a photodiode in the signal regulating circuit module 5 at the output end of the sample detection module 4, performs photoelectric signal conversion, and then obtains a detected signal by the data acquisition circuit module 6. Embedded system application software is preset in the main control circuit module 7, the main control circuit module 7 communicates with the LCD display screen 8 through an I/O interface, so that control of all parts of the instrument is realized, acquisition signals of the acquisition circuit module 6 are obtained, and the concentration of the detected parameter is calculated according to analysis and processing of the lambert beer law. Here, the embedded system application software preset in the main control circuit module 7 is an existing software system, and automatically calculates the detected concentration data.
The automatic optical detection analysis system of the present invention is shown in fig. 1, and the system adopts a single sample detection module 4, and when the detection of one parameter index is completed, the detection of the next parameter index is performed. The system light source adopts a monochromatic LED light source, the spectrum range is +/-10 nm, and the system light source is controlled by adopting a 20mA constant current source, and each light source can be independently controlled to be switched on and off. A certain point light source (a characteristic spectrum light source corresponding to the detected substance) is changed into a linear light beam through a lens after passing through the multi-in-one-out coupling optical fiber module 3, and then the linear light beam is injected into the light path channel inlet of the sample detection module 4. The width of the sample detection module 4 is the detection light path, and the light path in the system is 20mm. The photodiode is used as a photoelectric signal acquisition device, the intensity of an optical signal is converted into the corresponding current signal, a corresponding voltage signal is obtained through a small current-voltage conversion amplifying circuit, and the spectral sensitivity range of the photodiode adopted by the system is 400nm-1100nm.
The sample detection module of the invention works in a water bath constant temperature system, the water bath constant temperature system is a closed loop temperature control system, and when the temperature detection module works, the temperature controller 48 compares the real-time temperature acquired by the thermocouple temperature sensor 49 and then drives the heating pipe 47 to heat distilled water in the aluminum alloy shell, and the sample solution in the sample solution box 42 reaches a constant temperature under the action of heat transfer. Meanwhile, the stirring motor 410 rotates, and as the two magnets with opposite surface magnetic poles are arranged on the magnet tray 411, the magnetic field above the magnet tray 411 can be alternately changed, so that the stirring magnet 414 in the sample solution box 42 is driven to rotate, and the effect of stirring the sample solution is achieved.
The working flow of the optical system of the invention is as follows: if a three-part detection sample is provided, three monochromatic LED light sources are correspondingly used, namely an A monochromatic light source, a B monochromatic light source and a C monochromatic light source, when the concentration of a first sample parameter is detected, the A monochromatic light source is turned on, the B and C monochromatic light sources are turned off, only a parameter A characteristic spectrum light source exists in the light source entering the sample detection module 4 through the coupling optical fiber module 3, and at the moment, the multi-parameter optical system is changed into a single-parameter biochemical analysis optical system structure to finish the detection of the parameter A; and similarly, the second detection sample and the third detection sample are subjected to the same procedure to obtain the detection results of the parameters B and C. The core idea of the optical system structure of the multi-parameter biochemical analyzer is to utilize the multi-input and one-output coupling optical fiber to split light sources, the optical system has certain universality, and the system can be used for constructing a semi-automatic biochemical analyzer from single parameters to any multi-parameters only by changing the number of front-end light sources. The optical system adopts all fixed parts, has no movable part, greatly reduces the system error brought by the optical system, and ensures the measurement accuracy and repeatability of the whole instrument. The system has compact and simple structure and convenient assembly, and is very suitable for the design of miniaturized, portable and even hand-held semi-automatic biochemical analyzers; due to the structural variability, the method is very suitable for developing single-parameter to any multi-parameter serial products, and greatly shortens the development time and saves the development cost of the die.
It should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the technical solution, and although the applicant has described the present invention in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents of the technical solution of the present invention can be made without departing from the spirit and scope of the technical solution, and all such modifications and equivalents are intended to be encompassed in the scope of the claims of the present invention.
Claims (4)
1. The semi-automatic optical detection analysis system is characterized by comprising an optical detection system and a control system, wherein the optical detection system is composed of an LED driving circuit module (1), an LED light source (2), a coupling optical fiber module (3), a sample detection module (4) and a signal regulation circuit module (5), and the control system is composed of a data acquisition circuit module (6), a main control circuit module (7) and an LCD display screen (8); the LED driving circuit comprises a main control circuit module (7), an LED driving circuit module (1), a data acquisition circuit module (6), a sample detection module (4) and a display screen (8), wherein the input end of the LED driving circuit module (1) is connected with the output interface of the main control circuit module (7), the output end of the LED driving circuit module (1) is connected with the power input end of the LED light source (2), one side close to the LED light source (2) is provided with a coupling optical fiber module (3), the input end of the coupling optical fiber module (3) is opposite to the LED light source (2), the output end of the coupling optical fiber module (3) is connected with the input end of the sample detection module (4) for detecting light source signals, the output end of the sample detection module (4) is connected with the input end of the signal adjustment circuit module (5) through optical communication, the I/O interface on the signal adjustment circuit module (5) is in bidirectional communication with one I/O interface of the data acquisition circuit module (6), the other I/O interface of the data acquisition circuit module (6) is in bidirectional communication with one I/O interface of the main control circuit module (7), and the other I/O interface of the main control circuit module (7) is in bidirectional communication with the display screen (8); the sample detection module (4) comprises an aluminum alloy shell (41), a transparent sample solution box (42) is arranged in the aluminum alloy shell (41), the lower part of the sample solution box (42) is arranged in the aluminum alloy shell (41), the upper part of the sample solution box (42) extends out of the aluminum alloy shell (41), and distilled water is filled between the aluminum alloy shell (41) and the sample solution box (42); a left sleeve (43) is arranged on the left side of the aluminum alloy shell (41), a right sleeve (44) is arranged on the right side of the aluminum alloy shell (41), the left sleeve (43) and the right sleeve (44) are transversely embedded into the aluminum alloy shell (41), an outer opening of the left sleeve (43) and an outer opening of the right sleeve (44) are respectively in sealing connection with the aluminum alloy shell (41), and the inner sides of the left sleeve (43) and the right sleeve (44) are respectively tightly adhered to and in sealing connection with the sample solution tank (42); a left lens (45) is arranged at the outer opening of the left sleeve (43), a right lens (46) is arranged at the outer opening of the right sleeve (44), and the left lens (45) and the right lens (46) are positioned on the same horizontal plane and are opposite to each other; a heating pipe (47) is arranged in an aluminum alloy shell (41) at the bottom of a sample solution box (42), the input end of the heating pipe (47) is connected with the output end of a temperature controller (48) arranged at the outer side of the aluminum alloy shell (41) through a wire, the input end of the temperature controller (48) is electrically connected with a thermocouple temperature sensor (49) arranged on the aluminum alloy shell (41), and the probe of the thermocouple temperature sensor (49) is arranged in the aluminum alloy shell (41); the stirring motor (410) is further arranged below the aluminum alloy shell (41), an output shaft of the stirring motor (410) extends upwards and is fixedly connected with the magnet tray (411), an S-pole magnet (412) and an N-pole magnet (413) are arranged on the magnet tray (411), a rotatable stirring magnet (414) is arranged at the bottom of the inner side of the sample solution box (42), and the stirring magnet (414) is matched with the S-pole magnet (412) and the N-pole magnet (413); the LED light source (2) consists of at least three monochromatic LED lamps (21), and the monochromatic LED lamps (21) are respectively provided with an independent power supply control switch; the coupling optical fiber module (3) consists of at least three optical fiber input joints (31) and one optical fiber output joint (32), each optical fiber input joint (31) corresponds to a single-color LED lamp (21) respectively, and the optical fiber output joint (32) is connected with the input end of the sample detection module (4); a cuvette (415) is further arranged at the top of the sample solution box (42), the cross section of the left sleeve (43) and the cross section of the right sleeve (44) are elliptical, the left sleeve (43) is matched with the left lens (45), and the right sleeve (44) is matched with the right lens (46); the stirring magnet (414) is opposite to the output shaft of the stirring motor (410), and the S-pole magnet (412) and the N-pole magnet (413) are symmetrically arranged relative to the output shaft of the stirring motor (410).
2. The semiautomatic optical detection and analysis system according to claim 1, wherein the LED driving circuit module (1) comprises a power input terminal V1, the power input terminal V1 is respectively connected to one end of the resistor R1, one end of the resistor R2, and one end of the resistor R3, and the other end of the resistor R1 is connected to the diode D2 in forward direction and then connected to the cathode of the light emitting diode D4; the other end of the resistor R2 is connected with the cathode of the light emitting diode D3, the other end of the resistor R3 is connected with the diode D1 in the forward direction and then is respectively connected with one end of the capacitor C1, the power output end V2, the anode of the light emitting diode D3, the anode of the light emitting diode D4 and the anode of the light emitting diode D5, the other end of the capacitor C1 is grounded, and the cathode of the light emitting diode D5 is connected with the resistor R4 and then is grounded.
3. The semiautomatic optical detection and analysis system according to claim 2, wherein the signal conditioning circuit module (5) comprises a power input terminal V3, the power input terminal V3 is respectively connected to one end of the capacitor C2, the source of the fet D8, the source of the fet D7, the cathode of the photodiode D6, and one end of the resistor R5, the other end of the resistor R5 is respectively connected to the anode of the photodiode D6, the gate of the fet D7, and one end of the inductor L, the other end of the inductor L is respectively connected to one end of the inductor L2 and one end of the resistor R6, the other end of the inductor L2 is respectively connected to the drain of the fet D7 and the gate of the fet D8, and the other end of the resistor R6 is respectively connected to the drain of the fet D8, the other end of the capacitor C2, and the bidirectional communication interface U1.
4. The semiautomatic optical detection and analysis system according to claim 3, wherein the data acquisition circuit module (6) comprises a chip IC with a model of ADC0809CCN, a first pin of the chip IC is respectively connected to a fifth pin of the chip IC, one end of a resistor R12, one end of a resistor R10, and a negative electrode of a diode D9, an anode of the diode D9 is respectively connected to one end of a bidirectional communication interface U2 and one end of a resistor R8 after being connected to a resistor R7, the other end of the resistor R8 is respectively connected to one end of a capacitor C3 and one end of the resistor R9, the other end of the capacitor C3 is respectively connected to the other end of the resistor R9, one end of a capacitor C4, one end of a resistor R11, and one end of a capacitor C5 are respectively connected to one end of the other end of the resistor R11 and the other end of the resistor C5, the other end of the capacitor C5 is respectively connected to a fourth pin of the chip IC, a third pin of the chip IC is grounded, and a seventh pin of the chip IC is respectively connected to the other end of the resistor R12 and one end of the resistor R13, and the other end of the resistor R13 is connected to the bidirectional communication interface U3.
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