CN113875707B - Method for purifying local variety Yao silkworm resources of domestic silkworms with special purposes - Google Patents

Abstract

The invention discloses a method for selecting pure Yao silkworm resources of local varieties of silkworms with special purposes, which comprises the steps of collecting, grouping, detoxifying, purifying and subculturing. The method for selecting the local variety of the silkworm with special purposes, provided by the invention, comprises the steps of firstly, directly carrying out micro-particle disease quarantine on a female parent in the first generation of breeding, only keeping the silkworm eggs without micro-particle disease of the female moth for subculture breeding, screening out offspring produced by the female moth with virus preliminarily, and reducing the workload of selecting pure silkworm resources; then carrying out microparticle disease quarantine on the egg shell of the first generation of silkworm eggs to thoroughly screen out the silkworm eggs with virus, and further reducing the range of screening pure Yao silkworm; then continuously breeding and feeding, and according to the character characteristics of the Yao silkworm, eliminating the silkworm, cocoon and the like which do not accord with the character of the Yao silkworm while feeding, thereby quickly screening pure Yao silkworm resources.

Description

Purification method of Yao silkworm resource, a special-purpose landrace silkworm

technical field

The invention belongs to the technical field of breeding of special-purpose local silkworm varieties, and in particular relates to a method for purifying silkworm resources of special-purpose local silkworm varieties.

Background technique

Yao silkworm is an endemic silkworm species that is self-propagating, self-bred, and self-used by the Baiku Yao people. It is mainly distributed in Lihu Autonomous Township, Baxu Yao Autonomous Township, Hechi County, and Bagong Township, Hechi County, Guangxi Nandan County, Guangxi. Township. The Yao people in Baiku have been raising silkworms, and let the silkworms spun flat silk, which is used to make clothes. The clothes made of silk spun by Yao silkworms are very durable and not easy to break. The remaining silkworm chrysalis After turning into moths, they will mate and lay eggs naturally, and the silkworm eggs will be kept for future breeding and use. Secondly, "Nandan Yao silkworm flat silk" was rated as a geographical landmark product by the country in 2020, so Yao silkworm has precious scientific and commercial value.

However, the genotype of Yao silkworms tends to be homozygous after successive generations of self-breeding, the silkworms have poor resistance, and the survival rate of feeding is not high; in addition, the local people have poor conditions for raising silkworms, extensive management, and no quarantine for microsporidiosis. The resources of silkworm eggs of Yao silkworm are gradually decreasing, even on the verge of extinction. In addition, in order to take advantage of the commercial value of Yao silkworms, many businessmen sell silkworms of the yellow cocoon silkworm variety produced by outsiders to white trousers Yao cells for breeding, resulting in the mixing of local Yao silkworm varieties, and the fineness of the flat silk produced by Yao silkworms. and other good properties decline and gradually disappear.

Therefore, there is an urgent need for a method for selecting and purifying silkworm resources of special-purpose silkworm landraces that can obtain pure blood in hybrid silkworm varieties.

Contents of the invention

The object of the present invention is to solve at least the above-mentioned disadvantages and to provide advantages which will be explained later.

In order to achieve these objects and other advantages of the present invention, a method for purifying silkworm resources of a landrace silkworm for special purposes is now provided, including: collection and grouping, detoxification breeding, purification breeding and subculture.

Specifically include the following steps:

Collecting and grouping: In the area where Yao silkworm grows, the living silkworm resources are collected and numbered in groups on a household basis;

Virus-free rearing: Obtain silkworm eggs from the living silkworm resources and raise them in groups until they lay eggs. After the laying of eggs, the female moths are quarantined for microparticle disease, and only silkworm eggs produced by female moths without the pathogen of microparticle disease are kept; On the basis of the retained silkworm eggs, they are further accelerated until they are hatched to obtain ant silkworms, and the hatched egg shells are quarantined for microparticulate disease, and only the ant silkworms that do not carry the microparticulate disease pathogen in the egg shells are kept;

Purified rearing: Purify the obtained ant silkworms to obtain three-sleeping silkworms, continue to raise until cocoons form, eliminate white silk cocoons, keep yellow silk cocoons and continue to raise until laying eggs to obtain silkworm eggs, and keep the silkworm eggs for subsequent generations;

Subculture rearing: Households are used as a unit to carry out mixed breeding of silkworm eggs that have been reserved for subsequent generations. During the breeding process, the silkworms belonging to the households that have the four-sleep trait, single silkworm body color, or white cocoons are eliminated, and the remaining silkworms are kept. The silkworm chrysalis is produced to obtain pure silkworms.

In the above-mentioned scheme, the silkworm eggs of different farmers are collected firstly in the growing area of Yao silkworms, and the silkworm eggs of different farmers are collected; they are reared separately according to the household numbers, so as to lay the foundation for the subsequent screening of self-bred offspring without particulate pathogens.

Secondly, after one generation of breeding, the female parent is directly quarantined for microparticle disease, and only the silkworm eggs free from microparticle disease of the female moth are kept for subsequent reproduction, so as to preliminarily screen out the offspring produced by the infected female moth and reduce the workload of pure selection.

In addition, the ovum shells of the first generation of silkworm eggs are quarantined for microparticles, so as to thoroughly screen out the infected silkworm eggs and further narrow the scope of screening pure silkworms.

Finally, in the process of purified breeding and subculture, silkworms and cocoons that do not conform to the characteristics of Yao silkworms are eliminated while being raised according to the characteristics of Yao silkworms, so as to improve the efficiency of screening pure species of Yao silkworms.

Preferably, the live silkworm resources include: silkworms, silkworm cocoons, silkworm chrysalis and silkworm eggs.

In the above scheme, by collecting different silkworm egg materials to collect more silkworm egg resources owned by farmers, it lays the foundation for subsequent screening of self-bred offspring without particulate pathogens.

Preferably, the detoxification breeding specifically includes: S1, obtaining silkworm eggs from the living silkworm resources and dividing them into large groups according to the numbers and raising them until moths are grouped; S2, separating the female moths of each large group according to one female moth The groups are grouped and laid eggs, and silkworm eggs of multiple groups are obtained; S3, quarantine the female moths after the egg laying is over, and only keep the silkworm eggs of the group where the female moths without the pathogen of particulate disease belong; The silkworm eggs of the group are further accelerated until they hatch to obtain the ant silkworm, and the hatched egg shells are quarantined for microparticle disease; when an egg shell contains the pathogen of microparticle disease, all the ants of the group where the ant silkworm corresponding to the egg shell is located are eliminated. For silkworms, only the ant silkworms in the eggshells of the same group that did not carry the pathogen of microparticle disease were kept.

In the above scheme, the silkworm moths of the same household are divided into multiple groups for laying eggs by moths as a unit, so that when the eggshells of each egg are quarantined for microparticulate disease, the workload of microparticulate disease quarantine can be reduced and the screening can be done more quickly. Eliminate the ant silkworms carrying the microparticle disease pathogen, thereby improving the efficiency of screening pure silkworms.

Preferably, the purified rearing specifically includes: separately purifying and rearing the obtained ant silkworms on a household basis, and directly eliminating the silkworms belonging to the household where the silkworms belong to if any silkworms with four sleep characteristics are observed during the breeding process; the remaining silkworms continue to Breed until cocoons form, and if white cocoons appear, the silkworms belonging to the household corresponding to the white cocoons will be eliminated, and the remaining silkworms will continue to be raised until they lay eggs to obtain silkworm eggs. The silkworm eggs produced by the female moth without the pathogenic factor of microparticles disease are reserved for subsequent generation.

In the above scheme, the efficiency of screening pure silkworms is improved by purifying and breeding households and eliminating unqualified silkworms on a household basis.

The feeding conditions are as follows: the greening period: the temperature is 25°C, and the relative humidity is greater than 80%; the silkworm stage: the temperature is 25°C, and the relative humidity is 50-70%.

Preferably, the feeding includes: first-instar silkworms are fed with mulberry leaves cut into strips of 1 cm; second-instar silkworms are fed with mulberry leaves cut into triangles with a side length of 3-4 cm; third-instar silkworms and The fourth-instar silkworms were fed with mulberry leaves cut in half; among them, they were fed three times a day during the rearing process.

In the above scheme, feeding management is carried out according to the growth cycle of silkworms, so as to improve the survival rate of silkworm eggs.

Preferably, the rearing in the detoxification feeding and purification feeding also includes: when the silkworms are raised until they are mature, putting the cooked silkworms into a cocoon in a folding pile, picking the cocoons formed after six days in the upper bundle and putting them into a seed production grid Wait for the moths to come out; among them, only the same group of cooked silkworms is placed in one folding pile; only the same group of silkworm cocoons is placed in one breeding grid; the female moths and male moths in the same breeding grid are mated for more than 4 hours and then dismantled. Throw in moth circles placed on silkworm egg paper to lay eggs.

In the above-mentioned scheme, the survival rate of silkworm chrysalis is improved by putting cooked silkworms into folding piles to cocoon, so as to prevent broken species.

Preferably, the rearing in the subculture further includes: when the silkworms are raised until they are mature, putting the mature silkworms on a circular wooden board and weaving flat silk until they pupate naturally to obtain silkworm chrysalis; putting the obtained silkworm chrysalis into the seed production grid Wait for the moths to come out; among them, only the same group of silkworm cocoons are placed in one breeding grid; the female moths and male moths in the same breeding grid have been mated for more than 4 hours and then disassembled, and the female moths are thrown into the moth rings placed on the silkworm egg paper spawn in.

In the above scheme, in the process of subculture, the cooked silkworms are placed on a circular wooden board to spit out flat silk, so as to maintain the original characteristics of the Yao silkworm species to the greatest extent.

Preferably, the detoxification breeding is carried out in silkworm nests, wherein the specifications of the silkworm nests are: the length, width and height are 80 cm, 60 cm and 3 cm respectively, and one said silkworm nest has 20 grids in total, and the size of each grid is The specifications are length, width and height are 15 cm, 15 cm and 3 cm respectively.

Preferably, the feeding also includes: cutting the mulberry leaves into 1 cm strips, laying a film on the bottom of the silkworm nest, putting the cut mulberry leaves and first-instar silkworms into the silkworm nest and covering them with the film, wherein Put 20 first-year-old silkworms in one silkworm nest grid and feed them three times a day; sprinkle 10-20 grams of lime in one silkworm nest grid when the silkworms are sleeping; do not remove sand during the breeding process;

Cut the mulberry leaves into triangles with a side length of 3-4 cm, lay a film on the bottom of the silkworm nest, put the cut mulberry leaves and the second instar silkworms into the silkworm nest and cover them with the film, and put one of the silkworm nest 5 first-instar silkworms; feed three times a day, and transfer the second-instar silkworms and mulberry leaves with second-instar silkworms to a new clean grid after feeding twice a day; 10-20 grams of lime;

Cut the mulberry leaves in half, put the cut mulberry leaves and the third-instar silkworms into the silkworm nest, and put 2 third-instar silkworms in one of the silkworm nest grids, and feed them three times a day;

Cut the mulberry leaves in half, put the cut mulberry leaves or the whole mulberry leaves and the fourth-instar silkworms into the silkworm nest, and put a fourth-instar silkworm and adjacent fourth-instar silkworms in one of the silkworm nest grids. There is at least one grid at intervals; feed three times a day, and transfer the fourth-instar silkworm and the mulberry leaves with the fourth-instar silkworm to a new clean grid before each feeding to continue feeding.

In the above scheme, the silkworms are put into the silkworm nests for breeding, the small silkworms are mixed in a silkworm nest grid, and the large silkworms are raised in a single silkworm nest grid, which can effectively prevent cross-infection within a certain working range .

Advantages of the present invention:

First of all, the method for purifying the resources of the special-purpose silkworm local variety Yao silkworm of the present invention is to collect silkworm eggs in the growth area of Yao silkworm to collect as many silkworm eggs from different farmers as possible; Selfed offspring without microparticle pathogens lay the groundwork.

Secondly, the method for purifying silkworm resources of the special-purpose silkworm landrace of the present invention directly carries out microparticle disease quarantine on the female parents after one generation, and only retains the silkworm eggs free from microparticle disease of the female moth for subsequent reproduction, so as to preliminarily eliminate the microparticle disease. The offspring produced by female moths are screened out, reducing the workload of pure selection.

In addition, the method for purifying silkworm resources of the special-purpose silkworm local breed Yao silkworm of the present invention is to carry out microparticle disease quarantine on the egg shells of the first generation silkworm eggs, so as to thoroughly screen out the poisonous silkworm eggs and further reduce the size of the pure silkworm sieve. range of species.

In addition, the method for purifying silkworm resources of the special-purpose silkworm local variety Yao silkworm of the present invention, in the process of purifying feeding and subculture feeding, while feeding according to the characteristics of Yao silkworm, weed out silkworms and cocoons that do not meet the traits, so as to improve the screening process. The efficiency of purebred Yao silkworm.

In addition, the method for purifying silkworm resources of the special-purpose silkworm landrace of the present invention divides the silkworm moths of the same household into a plurality of groups to lay eggs by taking the moths as a unit, so as to carry out the microparticle disease quarantine on each eggshell that lays eggs At the same time, it can reduce the workload of microparticulate disease quarantine, and more quickly screen out the ant silkworms carrying the microparticulate disease pathogen, thereby improving the efficiency of screening pure silkworms.

In addition, the method for purifying silkworm resources of special-purpose silkworm landraces of the present invention improves the efficiency of screening pure Yao silkworms by purifying and raising them on a household basis and eliminating unqualified silkworms by households.

In addition, the method for purifying silkworm resources of the special-purpose silkworm landrace of the present invention, in the process of detoxification breeding, puts cooked silkworms into cocooning piles to improve the survival rate of silkworm chrysalis and prevent broken species.

In addition, the method for purifying the resources of the special-purpose silkworm local variety Yao silkworm of the present invention, in the process of subculture raising, puts the cooked silkworms on a circular wooden board and spins flat silk, so as to keep the quality of the Yao silkworm species to the greatest extent. original features.

Finally, the method for purifying silkworm resources of the special-purpose silkworm landrace of the present invention, puts silkworms into silkworm nests during the process of detoxification and purification breeding, and raises small silkworms in a silkworm nest grid. The single head of silkworms is reared in a silkworm nest grid, which can effectively prevent cross-infection within a certain working range.

Description of drawings

Fig. 1 is the structural representation of the silkworm nest in the method for purifying silkworm resources of special-purpose silkworm local variety Yao silkworm resources of the present invention;

Fig. 2 is the situation figure that utilizes the silkworm nest in the purifying method of silkworm local variety Yao silkworm resource of special purpose of the present invention to raise;

Fig. 3 is the situation diagram of the Yao silkworm that is obtained by the purification method of the special-purpose silkworm local variety Yao silkworm resources of the present invention on a log flat board.

Detailed ways

The present invention will be described in further detail below, so that those skilled in the art can implement it with reference to the description.

Example 1

A method for purifying silkworm resources of a landrace silkworm for special purposes, comprising: collection and grouping, detoxification breeding, purification breeding and subculture.

Step 1. Collection and grouping: In the Yao silkworm growth area, households are used as the unit to collect live silkworm resources and group and number them.

Step 2. Virus-free rearing: Obtain silkworm eggs from the living silkworm resources and raise them in groups until oviposition, perform quarantine on the female moths after oviposition, and only keep silkworms produced by female moths without the pathogen of microparticulate disease Eggs: On the basis of the retained silkworm eggs, they are further accelerated until they hatch to obtain the ant silkworms, and the hatched egg shells are quarantined for microparticulate disease, and only the ant silkworms that do not carry the microparticulate disease pathogen in the egg shells are kept.

Step 3. Purified breeding: Purify and raise the obtained ant silkworms to obtain three sleeper silkworms, continue to raise them until cocoons form, eliminate white silkworm cocoons, and keep yellow silkworm cocoons and continue to raise them until they lay eggs to obtain silkworm eggs, which are retained for subsequent generations .

Step 4, subculture breeding: take the household as the unit, carry out mixed breeding with the silkworm eggs that have been reserved for subsequent generations, and eliminate the silkworms owned by the households that have the four-sleep trait, single silkworm body color, or white silkworm cocoons in the breeding process. The preserved silkworm chrysalis is used for seed production to obtain the purebred silkworm.

Example 2

A method for purifying silkworm resources of a landrace silkworm for special purposes, comprising: collection and grouping, detoxification breeding, purification breeding and subculture.

Step 1. Collection and grouping: In the Yao silkworm growth area, households are used as the unit to collect live silkworm resources and group and number them.

Step 2. Virus-free rearing: S1. Obtain silkworm eggs from the living silkworm resources, divide them into large groups according to the numbers, and rear them until moths become moths.

S2. Group the female moths of each large group into groups according to one female moth and lay eggs, so as to obtain silkworm eggs of multiple groups.

S3. Quarantine the female moths after laying eggs for microparticulate disease, and only keep the silkworm eggs of the group where the female moths without the pathogen of microparticulate disease belong.

S4. Further catalyze the silkworm eggs in the reserved group until they hatch to obtain ants silkworms, and carry out quarantine on the hatched egg shells for microparticle disease; when an egg shell contains the pathogen of microparticles disease, eliminate the corresponding ants silkworm of the egg shell. All the silkworms of the same group were kept, and only the silkworms in the eggshells of the same group did not carry the pathogen of microparticle disease.

Step 3. Purification breeding: Purify and raise the obtained ant silkworms separately on a household basis. During the breeding process, if silkworms with four sleep characteristics are observed, all silkworms belonging to the household where the silkworms are located are directly eliminated; the remaining silkworms continue to be raised until they are finished. If white cocoons appear, the silkworms belonging to the household corresponding to the white cocoons will be eliminated. The remaining silkworms will continue to be raised until they lay eggs to obtain silkworm eggs. The silkworm eggs produced by the pathogenic female moth are reserved for subsequent generations.

Step 4, subculture breeding: take the household as the unit, carry out mixed breeding with the silkworm eggs that have been reserved for subsequent generations, and eliminate the silkworms owned by the households that have the four-sleep trait, single silkworm body color, or white silkworm cocoons in the breeding process. The preserved silkworm chrysalis is used for seed production to obtain the purebred silkworm.

Among them, in the process of detoxification breeding and purification breeding: when the silkworms are bred until they are mature, they are put into the folding cocoons to make cocoons, and after six days in the upper cocoons, the cocoons are picked and put into the seed production grid to wait for the moths to emerge. Among them, only the same group of cooked silkworms is placed in a folding pile; only the same group of silkworm cocoons is placed in a seed production grid; the female moths and male moths in the same breeding grid are mated for more than 4 hours and then disassembled, and the female moths are thrown into the set. Lay eggs in moth circles on silkworm seed paper.

In the process of subculture breeding: when the silkworms are bred to maturity, put the cooked silkworms on a circular wooden board and spit flat silk until they pupate naturally to obtain silkworm chrysalis; put the obtained silkworm chrysalis into the seed production grid to wait for moths to emerge; , only place the same group of silk cocoons in a breeding grid; the female moths and male moths in the same breeding grid have been mated for more than 4 hours and then disassembled, and the female moths are thrown into the moth circles placed on the silkworm egg paper to lay eggs.

In the feeding process of detoxification feeding, purification feeding and subculture feeding, the temperature and humidity conditions are greening stage: temperature is 25°C, relative humidity is greater than 80%; silkworm stage: temperature is 25°C, relative humidity is 50-70% .

Example 3

As shown in Figures 1 and 2, in a method for purifying silkworm resources of a special-purpose silkworm landrace, the silkworms reared for detoxification are raised in silkworm nests. Specifically, the specifications of the silkworm nests are:

The length, width and height of the silkworm nest are 80 centimeters, 60 centimeters and 3 centimeters respectively, and there are 20 silkworm nest grids 1 in one said silkworm nest, and the specifications of each silkworm nest grid 1 are 15 centimeters in length, 15 centimeters in width and 15 centimeters in height respectively. cm and 3 cm.

Example 4

A method for purifying silkworm resources of a landrace silkworm for special purposes, comprising: collection and grouping, detoxification breeding, purification breeding and subculture.

Step 1. Collection and grouping: In the Yao silkworm growth area, households are used as the unit to collect live silkworm resources and group and number them.

Step 2. Virus-free rearing: S1. Obtain silkworm eggs from the living silkworm resources, divide them into large groups according to numbers, and rear them until moths become moths.

The process of detoxification breeding includes: cutting mulberry leaves into 1 cm strips, laying a film on the bottom of the silkworm nest, putting the cut mulberry leaves and first-instar silkworms into the silkworm nest and covering them with the film. One of the silkworm nests Put 20 first-year-old silkworms in the grid and feed them three times a day; sprinkle 10-20 grams of lime in one silkworm nest grid when the silkworms are sleeping; do not remove sand during the breeding process.

Cut the mulberry leaves into triangles with a side length of 3-4 cm, lay a film on the bottom of the silkworm nest, put the cut mulberry leaves and the second instar silkworms into the silkworm nest and cover them with the film, and put one of the silkworm nest 5 first-instar silkworms; feed three times a day, and transfer the second-instar silkworms and mulberry leaves with second-instar silkworms to a new clean grid after feeding twice a day; 10-20 grams of lime.

Cut the mulberry leaves in half, put the cut mulberry leaves and third-instar silkworms into the silkworm nests, put 2 third-instar silkworms in one of the silkworm nest grids, and feed them three times a day.

Cut the mulberry leaves in half, put the cut mulberry leaves or the whole mulberry leaves and the fourth instar silkworms into the silkworm nest, and put a fourth instar silkworm in one of the silkworm nest grids, and the adjacent fourth instar silkworms There is at least one grid between them; feed three times a day, and before each feeding, transfer the fourth-instar silkworm and the mulberry leaves with the fourth-instar silkworm to a new clean grid to continue feeding.

When the silkworms are raised until they are mature, put the cooked silkworms into the cocooning piles, and put the cocoons into the seed-making grid after six days in the upper piles to wait for the moths to come out; wherein, only the same group of cooked silkworms is placed in one folding pile; Only place the same group of silkworm cocoons in a breeding grid; the female moths and male moths in the same breeding grid are mated for more than 4 hours before dismantling.

S2. Group the female moths of each large group into groups according to one female moth and put them in moth circles on the silkworm egg paper to lay eggs, and obtain silkworm eggs of multiple groups.

S3. Quarantine the female moths after laying eggs for microparticulate disease, and only keep the silkworm eggs of the group where the female moths without the pathogen of microparticulate disease belong.

S4. Further catalyze the silkworm eggs in the reserved group until they hatch to obtain ants silkworms, and carry out quarantine on the hatched egg shells for microparticle disease; when an egg shell contains the pathogen of microparticles disease, eliminate the corresponding ants silkworm of the egg shell. All the silkworms of the same group were kept, and only the silkworms in the eggshells of the same group did not carry the pathogen of microparticle disease.

Step 3. Purification breeding: Purify and raise the obtained ant silkworms separately on a household basis. During the breeding process, if silkworms with four sleep characteristics are observed, all silkworms belonging to the household where the silkworms are located are directly eliminated; the remaining silkworms continue to be raised until they are finished. If white cocoons appear, the silkworms belonging to the household corresponding to the white cocoons will be eliminated. The remaining silkworms will continue to be raised until they lay eggs to obtain silkworm eggs. The silkworm eggs produced by the pathogenic female moth are reserved for subsequent generations.

In the process of purifying breeding, when the silkworms are bred to maturity, they are put into the folding sacks to make cocoons, and six days after the upper sacks, the cocoons are collected and put into the seed-making lattice to wait for the moths to emerge; among them, one folding sack Only place the same group of mature silkworms in the same breeding cell; only place the same group of silkworm cocoons in the same breeding cell; the female moths and male moths in the same breeding cell have been mated for more than 4 hours and then disassembled, and the female moths are thrown into the cocoons placed on the silkworm egg paper. Lay eggs in moth rings.

Step 4, subculture breeding: take the household as the unit, carry out mixed breeding with the silkworm eggs that have been reserved for subsequent generations, and eliminate the silkworms owned by the households that have the four-sleep trait, single silkworm body color, or white silkworm cocoons in the breeding process. The preserved silkworm chrysalis is used for seed production to obtain the purebred silkworm.

In the process of subculture breeding: when the silkworms are bred to maturity, put the cooked silkworms on a circular wooden board and spit flat silk until they pupate naturally to obtain silkworm chrysalis; put the obtained silkworm chrysalis into the seed production grid to wait for moths to emerge; , only place the same group of silk cocoons in a breeding grid; the female moths and male moths in the same breeding grid have been mated for more than 4 hours and then disassembled, and the female moths are thrown into the moth circles placed on the silkworm egg paper to lay eggs.

In the feeding process of detoxification feeding, purification feeding and subculture feeding, the temperature and humidity conditions are greening stage: temperature is 25°C, relative humidity is greater than 80%; silkworm stage: temperature is 25°C, relative humidity is 50-70% .

test results

In Nandan County, Guangxi, 25 households of Yao silkworm resources were collected from farmers in the Baiku Yao settlement, and the method of Embodiment 4 was used to select and purify Yao silkworms. The situation of selecting pure silkworms is shown in Table 1:

Table 1:

According to the pure selection in Table 1, it can be seen that the silkworm eggs of groups 8, 10 and 17 were pure-bred silkworm eggs, and No. 17 was retained for subculture and laboratory preservation.

Although embodiments of the present invention have been disclosed above, it is not limited to the applications set forth in the specification and examples. It can be fully applied to various fields suitable for the present invention. Additional modifications can readily be made by those skilled in the art. Therefore, the invention is not to be limited to the specific details, without departing from the general concept defined by the claims and their equivalents.

Claims (7)

1. A method for purifying local variety Yao silkworm resources of domestic silkworms with special purposes is characterized by comprising the following steps:

collecting and grouping: collecting silkworm living body resources and grouping and numbering the silkworm living body resources by taking a family as a unit in a Yao silkworm growth area;

detoxification feeding: obtaining silkworm eggs from living body resources of the silkworms, feeding the silkworm eggs in groups until the silkworm eggs lay eggs, and carrying out micro-particle disease quarantine on female moths after the egg laying is finished, wherein only the silkworm eggs laid by the female moths without micro-particle disease pathogens are reserved; further carrying out incubation on the retained silkworm eggs until the hatched silkworm is hatched to obtain a silkworm, carrying out microparticle disease quarantine on the hatched egg shells, and only retaining the silkworm without the microparticle disease pathogen in the egg shells;

purifying and feeding: purifying and feeding the obtained ant silkworms to obtain trimolters, continuously feeding until cocoons are formed, eliminating white silkworm cocoons, keeping yellow silkworm cocoons, continuously feeding until the yellow silkworm cocoons lay eggs to obtain silkworm eggs, and reserving the silkworm eggs for subculture;

subculture breeding: taking households as a unit, carrying out mixed breeding on silkworm eggs reserved for subculture, eliminating all households with the four-sleep character and single silkworm body color or corresponding to white silkworm cocoons in the breeding process, and preparing seeds from the reserved silkworm pupas to obtain pure Yao silkworms;

the detoxification breeding specifically comprises the following steps:

s1, obtaining silkworm eggs from living silkworm resources, dividing the silkworm eggs into large groups according to numbers, and feeding the silkworm eggs to moth-killing groups in groups;

s2, grouping the female moths of each large group according to one female moth and one small group, and laying eggs to obtain multiple small groups of silkworm eggs;

s3, carrying out micro-particle disease quarantine on the female moths after the oviposition is finished, and only keeping the silkworm eggs of the group where the female moths without micro-particle disease pathogens are located;

s4, further carrying out incubation on the kept small groups of silkworm eggs until the kept small groups of silkworm eggs are incubated to obtain newly-hatched silkworms, and carrying out microparticle disease quarantine on the incubated egg shells; when one egg shell has the micro-particle disease pathogen, eliminating all the newly-hatched silkworms of the group where the newly-hatched silkworms corresponding to the egg shell are located, and only keeping the newly-hatched silkworms which do not have the micro-particle disease pathogen in the egg shell of the same group;

the detoxification breeding is carried out in silkworm nest, wherein the specifications of the silkworm nest are as follows:

the length, width and height are respectively 80 cm, 60 cm and 3 cm, 20 lattices are shared by 1 silkworm nest, and the specification of each lattice is that the length, width and height are respectively 15 cm, 15 cm and 3 cm;

raising further comprises:

cutting mulberry leaves into strips with the length of 1 cm, laying a film at the bottom of a silkworm nest, putting the cut mulberry leaves and one-year-old silkworms into the silkworm nest and covering the silkworm nest with the film, putting 20 first-year-old silkworms into one silkworm nest grid, and feeding the silkworms three times a day; spreading lime in 10-20 g in 1 nest grid when the silkworm is dormant; sand is not removed in the feeding process;

cutting mulberry leaves into a triangular shape with the side length of 3-4 cm, paving a film at the bottom of a silkworm nest, putting the cut mulberry leaves and two-instar silkworms into the silkworm nest and covering the silkworm nest with the film, wherein 5-first-instar silkworms are put into one silkworm nest grid; feeding for three times a day, and after feeding for two times, transferring the second-instar silkworms and mulberry leaves with the second-instar silkworms to a new clean grid for continuous feeding; spreading lime in 10-20 g in 1 nest grid when the silkworm is dormant;

cutting mulberry leaves into two halves, placing the cut mulberry leaves and three-instar silkworms into silkworm nests, placing 2-head three-instar silkworms into one silkworm nest grid, and feeding for three times a day;

putting two halves of mulberry leaves or a whole mulberry leaf and four-instar silkworms into silkworm nests, wherein 1 head of the four-instar silkworms are put into one silkworm nest grid, and at least one grid is arranged between every two adjacent four-instar silkworms; feeding for three times a day, and transferring four-instar silkworms and mulberry leaves with the four-instar silkworms to a new clean grid for continuous feeding before feeding each time.

2. The method for purifying the Yao silkworm resource of the local variety of the silkworm with the special application as claimed in claim 1, wherein the living silkworm resource comprises: silkworm, silkworm cocoon, silkworm pupa and silkworm seed.

3. The method for purifying local variety Yao silkworm resources of silkworms with special purposes of claim 1, wherein the purification breeding specifically comprises:

separately purifying and breeding the obtained newly-hatched silkworms by taking households as units, and directly eliminating all the silkworms of the households where the silkworms are positioned if silkworms with four-sleep characters appear in the breeding process; and (3) continuously feeding the rest silkworms to cocoons, eliminating all the silkworms of the house corresponding to the white silkworm cocoons if the white silkworm cocoons appear, continuously feeding the rest silkworms to lay eggs to obtain silkworm eggs, carrying out microparticle disease quarantine on the female moths after the egg laying is finished, and only reserving the silkworm eggs produced by the female moths without the microparticle disease pathogens for subculture.

4. The method for purifying local variety Yao silkworm resources of the domestic silkworm for special use as claimed in claim 1, wherein the raising conditions are as follows:

and (3) a hastening period: the temperature is 25 ℃ and the relative humidity is more than 80 percent;

the silkworm rearing period: the temperature is 25 ℃ and the relative humidity is 50 to 70 percent.

5. The method for purifying the local variety Yao silkworm resource of the special-purpose silkworm as claimed in claim 1, wherein the raising comprises:

feeding the first-instar silkworms with mulberry leaves cut into strips of 1 cm;

feeding two-year-old silkworms with triangular mulberry leaves with side lengths of 3-4 cm;

feeding three-instar silkworms and four-instar silkworms with mulberry leaves cut into two halves;

wherein, the feeding process is carried out three times a day.

6. The method for purifying the Yao silkworm resource of the local variety of the silkworm as claimed in claim 1, wherein the breeding for virus-free breeding and purification breeding further comprises:

when the mature silkworms are raised, placing the mature silkworms into a cocoon folding frame for cocooning, picking the cocoons after six days of cocooning and placing the cocoons into a seed production grid for moth emergence; wherein, only the same group of boiled silkworms are placed in one cocoon folding frame; only the same group of silkworm cocoons are placed in one seed making grid;

mating female moth and male moth in the same seed making lattice for more than 4 hr, separating, and laying eggs in moth ring on silkworm seed paper.

7. The method for purifying local variety Yao silkworm resources of a special purpose silkworm as claimed in claim 1, wherein the raising in the subculture further comprises:

when the silkworm is bred to be mature, the mature silkworm is placed on the round wood board, and flat silk is spitted until the silkworm pupae naturally melt, so that the silkworm pupae are obtained;

putting the silkworm chrysalis into a seed production grid to obtain moths; wherein, only the same group of silkworm cocoons are placed in one seed making grid;

mating female moth and male moth in the same seed making lattice for more than 4 hr, separating, and laying eggs in moth ring on silkworm seed paper.

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