CN113768862B

CN113768862B - Anti-wrinkle composition, preparation method and application thereof

Info

Publication number: CN113768862B
Application number: CN202111194487.8A
Authority: CN
Inventors: 崔英云; 李传茂; 张伟杰; 曾伟丹; 张楚标
Original assignee: Guangdong Danz Group Co Ltd; Guangzhou Keneng Cosmetic Research Co Ltd
Current assignee: Guangdong Danz Group Co Ltd; Guangzhou Keneng Cosmetic Research Co Ltd
Priority date: 2021-10-13
Filing date: 2021-10-13
Publication date: 2023-10-20
Anticipated expiration: 2041-10-13
Also published as: CN113768862A

Abstract

The invention provides an anti-wrinkle composition, and a preparation method and application thereof. The anti-wrinkle composition of the present invention comprises the following composition: an extract of spirulina platensis; palmitoyl tripeptide-5; dipeptide diamino Ding Xianbian-yl amide diacetate; the content of the Bonateglinide spirulina extract is 0.001% -5%, the content of the palmitoyl tripeptide-5 is 0.001% -0.05% and the content of the dipeptide diamino Ding Xianbian-based amide diacetate is 0.001% -0.05% based on 100% of the total mass of the anti-wrinkle composition. The anti-wrinkle composition of the invention can improve the wrinkle problem from multiple angles in all directions. The invention achieves the effect which is obviously superior to that of single or simple combination by combining and compounding the Bonatte spirulina extract, palmitoyl tripeptide-5 and dipeptide diamino Ding Xianbian-base amide diacetate, thereby realizing the effects of enhancing skin elasticity and compactness and reducing fine wrinkles.

Description

Anti-wrinkle composition, preparation method and application thereof

Technical Field

The invention belongs to the field of cosmetics, and in particular relates to an anti-wrinkle composition, a preparation method and application thereof.

Background

Skin, like other organs, gradually tends to age with age. Aging of skin is a normal phenomenon, which is always present from our birth, and as a result of aging, it is most important that the appearance of the human body is changed in addition to the physiological function of the skin is reduced. People often judge the age, identity and status of a person through some external features of skin and hair. Aging causes the human body to beautify and reduce the color, so that the appearance of aging can have negative effects on the mind of the human body, and the human body hopes to keep the appearance young and youthful. Preventing and delaying skin aging is an important topic of cosmetic skin science. More affected by exogenous stimuli than other organs, and thus aging of the skin is a result of the combined effects of endogenous and exogenous stimuli.

The skin is a largest organ which covers the whole body surface, is a protector of internal organs and tissues of a human body, is also an effect organ of the internal organs, the spirit and the surrounding environment, and plays an important role in the health and the beauty of the human body. Anti-aging skin care products are one of the main functions of skin care products, and the gene cosmetology of modern biotechnology achievements is utilized, so that the effects of improving the skin quality, delaying aging and reducing wrinkles from the aspect of skin cell structures have become a worldwide trend.

How to care the skin, restore the moist and elastic properties of the skin, and resist or delay aging as much as possible is a hotspot in the art. On one hand, people can improve the skin state by reasonably adjusting the diet structure, insisting on drinking water and the like, eating fruits and not overstrain and the like; on the other hand, it is also important to enhance the daily care of the skin.

Therefore, how to develop skin care compositions effective in improving wrinkles is a technical problem to be solved.

Disclosure of Invention

Problems to be solved by the invention

In view of the deficiencies of the prior art, for example: the invention aims to provide an anti-wrinkle composition which can effectively improve the problem of wrinkles.

Furthermore, the invention also provides a preparation method of the anti-wrinkle composition, which is simple and feasible, and raw materials are easy to obtain.

Solution for solving the problem

The invention provides an anti-wrinkle composition comprising the following composition:

an extract of spirulina platensis;

palmitoyl tripeptide-5;

dipeptide diamino Ding Xianbian-yl amide diacetate;

the content of the Bonateglinide spirulina extract is 0.001% -5%, the content of the palmitoyl tripeptide-5 is 0.001% -0.05% and the content of the dipeptide diamino Ding Xianbian-based amide diacetate is 0.001% -0.05% based on 100% of the total mass of the anti-wrinkle composition.

The anti-wrinkle composition according to claim 1, wherein the content of the bolsteride spirulina extract is 0.001% -3%, the content of the palmitoyl tripeptide-5 is 0.001% -0.04%, and the content of the dipeptide diamino Ding Xianbian-ylamide diacetate is 0.001% -0.04% based on 100% of the total mass of the anti-wrinkle composition.

The anti-wrinkle composition according to the present invention, wherein the content of the Bonatate spirulina extract is 0.001% -2%, the content of the palmitoyl tripeptide-5 is 0.001% -0.03% and the content of the dipeptide diamino Ding Xianbian-ylamide diacetate is 0.001% -0.03% based on 100% of the total mass of the anti-wrinkle composition.

The anti-wrinkle composition according to the present invention, wherein the content of the Bonateglinide spirulina extract is 0.001% -1.5%, the content of the palmitoyl tripeptide-5 is 0.001% -0.02%, and the content of the dipeptide diamino Ding Xianbian-ylamide diacetate is 0.001% -0.02% based on 100% of the total mass of the anti-wrinkle composition.

The anti-wrinkle composition according to the present invention, wherein the anti-wrinkle composition further comprises one or a combination of two or more of a humectant, a chelating agent, a preservative, and a solvent; the humectant is added in an amount of 1% -50% based on 100% of the total mass of the anti-wrinkle composition; the addition amount of the chelating agent is 0.01-1%; the addition amount of the preservative is 0.1-1%, and the addition amount of the solvent is 22-95%.

The anti-wrinkle composition according to the present invention, wherein the humectant comprises one or a combination of two or more of 1, 3-propanediol, propylene glycol, pentanediol, hexanediol, sorbitol, glycerin, dipropylene glycol, 1, 3-butanediol, trehalose, xylitol, erythritol, glycereth-26, β -glucan, methylgluceth-20, methylgluceth-10, erythrulose, bioglycol-1, betaine, rhamnose, oat β -glucan, acetylglucosamine, polyethylene glycols, water-soluble oil, water-soluble silicone oil, and sodium hyaluronate.

The anti-wrinkle composition according to the present invention, wherein the chelating agent comprises EDTA-2Na and/or EDTA-4Na; and/or the preservative is one or the combination of more than two of hydroxyacetophenone, phenoxyethanol, sorbitan octoate, benzyl alcohol, benzoic acid, gluconolactone, sodium benzoate and ethylhexyl glycerol.

The present invention also provides a method for preparing the anti-wrinkle composition according to the present invention, which comprises the step of mixing the components of the anti-wrinkle composition.

The invention also provides the use of an anti-wrinkle composition according to the invention for the preparation of a skin care product; preferably, the skin care product is one of eye cream, eye gel, eye mask, eye essence, eye patch, face cream, face cleansing, skin lotion, essence, skin care cream, facial mask, neck cream, lyophilized powder and sun protection.

The use according to the invention, wherein the anti-wrinkle composition is added in an amount of 1% to 90%.

ADVANTAGEOUS EFFECTS OF INVENTION

The anti-wrinkle composition of the invention can improve the wrinkle problem from multiple angles in all directions. The invention achieves the effect which is obviously superior to that of single or simple combination by combining and compounding the Bonatte spirulina extract, palmitoyl tripeptide-5 and dipeptide diamino Ding Xianbian-base amide diacetate, thereby realizing the effects of enhancing skin elasticity and compactness and reducing fine wrinkles.

In addition, the preparation method of the anti-wrinkle composition is simple and feasible, raw materials are easy to obtain, and the anti-wrinkle composition is suitable for mass production.

The eye care product containing the anti-wrinkle composition, which is prepared by the invention, is safe and non-irritating, can effectively improve the eye wrinkles, and is suitable for people of all ages.

Detailed Description

The following describes the present invention in detail. The following description of the technical features is based on the representative embodiments and specific examples of the present invention, but the present invention is not limited to these embodiments and specific examples.

Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better illustration of the invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In other instances, well known methods, procedures, means, equipment and steps have not been described in detail so as not to obscure the present invention.

It should be noted that:

in the present specification, the use of the meaning of "can" or "can" includes both the meaning of performing a certain process and the meaning of not performing a certain process.

In the present specification, the numerical range indicated by the use of "numerical value a to numerical value B" means a range including the end point numerical value A, B.

Unless otherwise indicated, all units used in the present invention are international standard units, and numerical values, ranges of values, appearing in the present invention should be understood to include errors permitted in industrial production.

Reference throughout this specification to "some specific/preferred embodiments," "other specific/preferred embodiments," "an embodiment," and so forth, means that a particular element (e.g., feature, property, and/or characteristic) described in connection with the embodiment is included in at least one embodiment described herein, and may or may not be present in other embodiments. In addition, it is to be understood that the elements may be combined in any suitable manner in the various embodiments.

In the present specification, if "room temperature", "normal temperature" or the like occurs, the meaning may be "10 to 40 ℃.

In a first aspect the present invention provides an anti-wrinkle composition comprising the following composition:

an extract of spirulina platensis;

palmitoyl tripeptide-5;

dipeptide diamino Ding Xianbian-yl amide diacetate;

the content of the Bonatte spirulina extract is 0.001% -5%, the content of the palmitoyl tripeptide-5 is 0.001% -0.05%, and the content of the dipeptide diamino Ding Xianbian-based amide diacetate is 0.001% -0.05%.

In the invention, the raw materials of each component are mild to human body, safe and non-irritating. The invention uses the compatibility of the Bonatte spirulina extract, palmitoyl tripeptide-5 and dipeptide diamino Ding Xianbian-base amide diacetate, and the three exert the synergistic effect.

Further, in view of enabling the synergistic effect to be effectively exerted, the mass ratio of the bolsterine spirulina extract, palmitoyl tripeptide-5, and dipeptide diamino Ding Xianbian-based amide diacetate of the present invention may be 0.1 to 200:0.5 to 50:1, for example: 1-180:0.6-45:1, 1.5-160:0.7-40:1, 2-140:0.8-35:1, 2.5-120:0.8-30:1, 3-100:0.9-25:1, 3.5-90:1-20:1, and the like.

Bonatate spirulina extract

In the present invention, the Bonatate spirulina extract belongs to the genus of cyanobacteria, phalaales, oscillatoriaceae, spirulina. Since the 60 s of the last century, spirulina has been progressively discovered for its abundant and unique bioactive components and functions. Such as protein, gamma-linolenic acid, phycocyanin, beta-carotene, inositol, spirulina polysaccharide, essential amino acids, vitamin B12 and the like, has various biological activities in improving malnutrition, supplementing protein, resisting oxidation, enhancing immunity, improving gastrointestinal function, reducing blood fat and the like, and has become internationally accepted super nutrition and health care food. The Bonatal spirulina extract contains Bonatal spirulina polysaccharide which is an acidic heteropolysaccharide and is used as an important physiologically active substance of spirulina, and has good effects in the aspects of antioxidation, DNA damage repair and the like.

In the present invention, the bolsteri spirulina extract may be extracted by an acid agent. Specifically, the acid agent may be obtained by dissolving an acidic substance in a solvent. The acidic substance and the solvent are not particularly limited, and may be ones commonly used in the art. For example: the acidic substance can be one or more than two of sulfuric acid, hydrochloric acid, acetic acid, phosphoric acid, nitric acid and the like; the solvent may be water or the like. The concentration of the acidic substance in the acid agent is not particularly limited, and may be set as required, for example, 0.1 to 5mol/L or the like.

In some embodiments, the powder of Bonateglinide is placed in an acid, heated in a water bath at a temperature above 50 ℃, preferably 50-100 ℃, and after a certain period of time, the supernatant is collected to obtain a total sugar extract of Bonateglinide.

The method of collecting the clear liquid is not particularly limited, and the method of centrifugal separation may be adopted, and the remaining lower layer solid matter may be further extracted by the method of centrifugal separation. Specifically, the rotational speed of the centrifugal separation can be 6000-10000r/min, and the time of the centrifugal separation can be 5-20min.

After centrifugation, the above method can be repeated, and the spirulina powder is repeatedly extracted for 2-5 times to fully obtain the total sugar extract of Bonatate spirulina. And then drying the total sugar extract of the Bonatal spirulina to obtain the Bonatal spirulina extract.

In the invention, the Bonatte spirulina extract prepared by the preparation method has strong antioxidation, can restore the elasticity and compaction of skin, and has various effects of brightening skin color, refining wrinkles and the like.

In the present invention, the content of the spirulina platensis extract is 0.001% to 5%, preferably 0.001% to 3%, more preferably 0.001% to 2%, still more preferably 0.001% to 1.5%, based on 100% of the total mass of the anti-wrinkle composition, for example: 0.005%, 0.01%, 0.05%, 0.1%, 0.5%, 1.5%, 2.5%, 3.5%, 4.5%, etc. When the content of the Bonatate spirulina extract is less than 0.001%, the corresponding effect cannot be achieved, and when the content of the Bonatate spirulina extract is more than 5%, the cost is too high.

Palmitoyl tripeptide-5

Palmitoyl tripeptide-5, a signaling peptide that promotes collagen (type I, type II, and type IV) synthesis and extracellular matrix protein production by increasing stromal cell activity, making the skin appear younger and more elastic. Has the effects of promoting collagen and elastin synthesis in dermis, accelerating collagen generation and repairing wrinkles; therefore, the palmitoyl tripeptide-5 can help skin to supplement collagen, promote facial elasticity and remarkably reduce facial wrinkles. Meanwhile, the palmitoyl tripeptide-5 also has the characteristics of enhancing cell activity, improving skin moisture content, enhancing skin luster and improving skin color.

In the present invention, the palmitoyl tripeptide-5 is contained in an amount of 0.001% to 0.05%, preferably 0.001% to 0.04%, more preferably 0.001% to 0.03%, still more preferably 0.001% to 0.02%, based on 100% by weight of the total anti-wrinkle composition, for example: 0.002%, 0.004%, 0.006%, 0.008%, 0.01%, 0.015%, 0.02%, 0.025%, 0.03%, 0.035%, 0.04%, 0.045% and the like. When the content of palmitoyl tripeptide-5 is less than 0.001%, the effect of reducing fine wrinkles cannot be achieved correspondingly, and when the content of palmitoyl tripeptide-5 is more than 0.05%, the cost is too high.

Dipeptide diamino Ding Xianbian-yl amide diacetate

Dipeptide diamino Ding Xianbian-based amide diacetate simulates the function of toxoid, and can realize local blocking of nerve transmission muscle contraction information by combining with a muscle nicotinic acetylcholine receptor (mnAChR) so as to relax facial muscles and achieve the aim of smoothing fine wrinkles. The peptides are widely applied as signal peptides, and are particularly suitable for being applied to the concentrated positions (canthus, neck lines and facial lines) of expression muscles.

In the present invention, the content of the dipeptide diamino Ding Xianbian-based amide diacetate is 0.001% to 0.05%, preferably 0.001% to 0.04%, more preferably 0.001% to 0.03%, still more preferably 0.001% to 0.02%, based on 100% of the total mass of the anti-wrinkle composition, for example: 0.002%, 0.004%, 0.006%, 0.008%, 0.01%, 0.015%, 0.02%, 0.025%, 0.03%, 0.035%, 0.04%, 0.045% and the like. When the content of the dipeptide diamino Ding Xianbian-based amide diacetate is less than 0.01%, the corresponding anti-wrinkle effect cannot be achieved, and when the content of the dipeptide diamino Ding Xianbian-based amide diacetate is more than 0.05%, the cost is too high.

In the invention, three components of the Bonatte spirulina extract, the palmitoyl tripeptide-5 and the dipeptide diamino Ding Xianbian-based amide diacetate are used, which can play a role in synergy, thereby achieving the effects of reducing fine wrinkles, promoting the synthesis of collagen and elastin and resisting oxidation.

Other components

In the present invention, the anti-wrinkle composition further comprises one or a combination of two or more of a humectant, a chelating agent, a preservative, a solvent, and the like; the humectant is added in an amount of 1% -50% based on 100% of the total mass of the anti-wrinkle composition; the addition amount of the chelating agent is 0.01-1%; the addition amount of the preservative is 0.1-1%, and the addition amount of the solvent is 22-95%.

The humectant is added, so that the anti-wrinkle composition has excellent moisturizing performance on the basis of improving fine wrinkles. In the present invention, the humectant is added in an amount of 1% to 50%, preferably 1% to 25%, more preferably 1% to 20%, based on 100% of the total mass of the anti-wrinkle composition; in order to further exert the efficacy of the humectant, the humectant of the present invention may be added in an amount of 1 to 18%, 3 to 16%, 5 to 14% or 6 to 12%. For example: may be 2%, 3%, 6%, 8%, 10%, 12%, 15%, 17%, 19%, etc.

Specifically, the humectant comprises one or more than two of 1, 3-propylene glycol, pentanediol, hexanediol, sorbitol, glycerol, dipropylene glycol, 1, 3-butanediol, trehalose, xylitol, erythritol, glycereth-26, beta-glucan, methylgluceth-20, methylgluceth-10, erythrulose, biogel-1, betaine, rhamnose, oat beta-glucan, acetylchitosan, polyethylene glycols, water-soluble oil, water-soluble silicone oil and sodium hyaluronate.

Further, in the present invention, the chelating agent and the preservative are not particularly limited, and may be those commonly used in the art. The chelating agent is added in an amount of 0.01 to 1%, preferably 0.01 to 0.1%, based on 100% of the total mass of the anti-wrinkle composition; the addition amount of the preservative is 0.1-1%. For example, the chelating agent includes EDTA-2Na and/or EDTA-4Na. The preservative is one or the combination of more than two of hydroxyacetophenone, phenoxyethanol, sorbitan octoate, benzyl alcohol, benzoic acid, gluconolactone, sodium benzoate and ethylhexyl glycerol.

In addition, the solvent to be used in the present invention is not particularly limited, and may be any solvent available in the art, for example: water, and the like. As for water, there may be some water commonly used in the art, such as: purified water, deionized water, distilled water, double distilled water, ion-exchanged water, sterile water, ultrapure water, and the like. Specifically, the content of the solvent may be 22 to 95%, for example: the solvent may be added in an amount of 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 75%, 80%, 85%, 90%, etc.

The anti-wrinkle composition provided by the invention has various functional components, and besides the complex combination of the Bonatte spirulina extract, the palmitoyl tripeptide-5 and the dipeptide diamino Ding Xianbian-based amide diacetate serving as main functional components, one or more of a humectant, a chelating agent, a preservative and a solvent are also related components, and can be taken as a whole, so that the anti-wrinkle composition capable of effectively improving fine wrinkles is prepared.

The anti-wrinkle composition has the effects of moisturizing, reducing fine wrinkles and tightening skin, and the active ingredients can penetrate into the muscle bottoms of eyes to care the skin of the eyes.

< second aspect >

In a second aspect, the present invention provides a method of preparing an anti-wrinkle composition, comprising the step of mixing the components of the anti-wrinkle composition. The preparation method of the anti-wrinkle composition is simple and feasible, raw materials are easy to obtain, and the anti-wrinkle composition is suitable for mass production.

In some specific embodiments, the method of preparing the anti-wrinkle composition comprises the steps of:

1) Adding the optional humectant, the optional chelating agent, part of the optional preservative and the optional water into an emulsifying pot, heating to 80-85 ℃, and stirring for dissolution;

2) Cooling to 42-48deg.C, adding Bonatte spirulina extract, palmitoyl tripeptide-5, dipeptide diamino Ding Xianbian base amide diacetate and the rest optional antiseptic, and stirring to obtain anti-wrinkle composition.

Further, the invention also provides an application of the anti-wrinkle composition in preparing skin care products such as anti-wrinkle products; preferably, the skin care product can be one of eye cream, eye gel, eye mask, eye essence, eye patch, face cream, face cleaning, skin care water, essence, skin care cream, facial mask, neck cream, freeze-dried powder, sun protection and the like.

Specifically, the anti-wrinkle composition is added in an amount of 1% to 99% by weight of the total mass of the skin care product, for example: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, etc.

Further, when the anti-wrinkle product is used as an eye care product, such as eye cream, eye essence, eye gel, etc., the anti-wrinkle composition is added in an amount of 1% to 50% based on the total mass of the eye care product; when the eye care product is an eye mask, eye patch, or the like, the anti-wrinkle composition is added in an amount of 1% -20%.

Examples

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

In the examples, the extraction process of the Bonatate spirulina extract is as follows:

weighing 10mg of algae powder, adding 5mL of 0.5mol/L H ₂ SO ₄ In the above, the mixture was stirred in a water bath at 80℃for 0.5h, and then centrifuged at 8000r/min for 10min, and the supernatant was collected. Repeatedly extracting for 3 times, mixing the supernatants, fixing volume to 50mL to obtain total sugar extract, and air drying and drying to obtain Bonatate spirulina extract.

Examples 1 to 10

Anti-wrinkle compositions of examples 1-10 were prepared according to the mass percentages of the components in tables 1-2 below.

Wherein, the preparation method of the anti-wrinkle composition comprises the following steps:

1) Adding sterilized deionized water into main pot, adding hyaluronic acid, glycerol, 1, 3-propylene glycol, betaine, EDTA-2NA, and methylparaben under stirring, heating to 82 deg.C, homogenizing at 3000rpm for 3min, stirring until no block is formed, and maintaining the temperature for 20min.

2) Cooling to 45deg.C, adding Bonatte spirulina extract, palmitoyl tripeptide-5, and dipeptide diamino Ding Xianbian base amide diacetate, phenoxyethanol, stirring, cooling to 35deg.C, inspecting, and discharging.

Comparative examples 1 to 7

According to the following preparation methods of examples 1 to 10, anti-wrinkle compositions of comparative examples 1 to 7 were prepared according to the mass percentages of the respective components in table 3.

TABLE 1 anti-wrinkle compositions of examples 1-5

TABLE 2 anti-wrinkle compositions of examples 6-10

TABLE 3 anti-wrinkle compositions of comparative examples 1-7

Anti-wrinkle Performance test

The detection method for the crease resistance test is as follows:

1.1 subject screening criteria

Healthy female subjects between 20 and 50 years of age; and the subjects are all nonsensitive vaccinia muscles; the number of people is 85.

1.2 application sites: periocular region

1.3 frequency of use: the medicine is used for 1 time in the morning and at night, and is used for 28 days continuously, and 0.8g of medicine is used each time.

1.4 test environmental conditions

The temperature is 21+/-1 ℃ and the relative humidity is 50+/-5%;

1.5 test design

Single blind, random, self front-back contrast, face (left/right measuring area is selected according to random table)

1.6 evaluation method details

The under-eye lines were analyzed using Primos CR image acquisition and analysis, as set forth in table 4 below.

TABLE 4 Table 4

From the results of Table 4, it is seen that there is a tendency for the number of fine lines to be improved as the mass percentage of Bonatte spirulina extract, palmitoyl tripeptide-5, and dipeptidyl diamino Ding Xianbian-ylamide diacetate in the formulation is gradually increased in examples 1 to 10. From example 1 and comparative examples 1-7, it is seen that the combination and compounding of Bonatte spirulina extract, palmitoyl tripeptide-5, and dipeptide diamino Ding Xianbian-based amide diacetate can achieve an effect significantly better than that achieved by single or simple combination, and achieve the effect of reducing fine wrinkles.

Therefore, the composition of the present invention can be used to improve wrinkles.

The above examples of the present invention are only examples for clearly illustrating the present invention, and are not limiting of the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.

Claims

1. An anti-wrinkle composition, comprising the following composition:

an extract of spirulina platensis;

palmitoyl tripeptide-5;

dipeptide diamino Ding Xianbian-yl amide diacetate;

the content of the Bonateglinide spirulina extract is 0.001% -5%, the content of the palmitoyl tripeptide-5 is 0.001% -0.05% and the content of the dipeptide diamino Ding Xianbian-based amide diacetate is 0.001% -0.05% based on 100% of the total mass of the anti-wrinkle composition;

the mass ratio of the Bonatte spirulina extract to the palmitoyl tripeptide-5 to the dipeptide diamino Ding Xianbian-based amide diacetate is 3.5-90:1-20:1;

the Bonatate spirulina extract is obtained by extracting with an acid agent.

2. The anti-wrinkle composition according to claim 1, wherein the content of the bolsteride spirulina extract is 0.001% -3%, the content of the palmitoyl tripeptide-5 is 0.001% -0.04%, and the content of the dipeptide diamino Ding Xianbian-ylamide diacetate is 0.001% -0.04% based on 100% of the total mass of the anti-wrinkle composition.

3. The anti-wrinkle composition according to claim 2, wherein the content of the bolsteride spirulina extract is 0.001% -2%, the content of the palmitoyl tripeptide-5 is 0.001% -0.03%, and the content of the dipeptide diamino Ding Xianbian-ylamide diacetate is 0.001% -0.03% based on 100% of the total mass of the anti-wrinkle composition.

4. The anti-wrinkle composition according to claim 3, wherein the content of the Bonateglinide spirulina extract is 0.001% -1.5%, the content of the palmitoyl tripeptide-5 is 0.001% -0.02%, and the content of the dipeptide diamino Ding Xianbian-based amid diacetate is 0.001% -0.02% based on 100% of the total mass of the anti-wrinkle composition.

5. The anti-wrinkle composition according to any one of claims 1-4, further comprising one or a combination of two or more of a humectant, a chelating agent, a preservative, and a solvent; the humectant is added in an amount of 1% -50% based on 100% of the total mass of the anti-wrinkle composition; the addition amount of the chelating agent is 0.01-1%; the addition amount of the preservative is 0.1-1%, and the addition amount of the solvent is 22-95%.

6. The anti-wrinkle composition according to claim 5, wherein the humectant comprises one or more of 1, 3-propanediol, propylene glycol, pentanediol, hexanediol, sorbitol, glycerin, dipropylene glycol, 1, 3-butanediol, trehalose, xylitol, erythritol, glycereth-26, β -glucan, methylgluceth-20, methylgluceth-10, erythrulose, bioglycol-1, betaine, rhamnose, oat β -glucan, chitosan, polyethylene glycols, water-soluble oils, water-soluble silicone oils, and sodium hyaluronate.

7. The anti-wrinkle composition according to claim 5, wherein the chelating agent comprises EDTA-2Na and/or EDTA-4Na; and/or the preservative is one or the combination of more than two of hydroxyacetophenone, phenoxyethanol, sorbitan octoate, benzyl alcohol, gluconolactone and ethylhexyl glycerol.

8. A method of preparing an anti-wrinkle composition according to any one of claims 1-7, comprising the step of mixing the components of the anti-wrinkle composition.

9. Use of an anti-wrinkle composition according to any one of claims 1-7 for the preparation of a skin care product.

10. The use according to claim 9, wherein the skin care product is one of an eye cream, an eye gel, an eye mask, an eye patch, a face cream, a face cleansing, a lotion, an essence, a skin cream, a mask, a neck cream, a freeze-dried powder, and a sun protection.

11. The use according to claim 9, wherein the skin care product is an eye essence.

12. Use according to any one of claims 9 to 11, wherein the anti-wrinkle composition is added in an amount of 1% to 90%.