CN116327674A

CN116327674A - Anti-wrinkle compact micro-nano essence emulsion and preparation method thereof

Info

Publication number: CN116327674A
Application number: CN202310410170.6A
Authority: CN
Inventors: 刘金坤; 房凡仁; 李晓; 闫越; 高帅
Original assignee: Shandong Anran Nanometre Ind Development Co ltd
Current assignee: Shandong Anran Nanometre Ind Development Co ltd
Priority date: 2023-04-12
Filing date: 2023-04-12
Publication date: 2023-06-27

Abstract

The invention discloses anti-wrinkle compact micro-nano essence emulsion and a preparation method thereof. Comprises the following components in parts by weight: 0.005-0.1 part of ginseng tissue culture, 0.1-5 parts of PEG/PPG-70/30 tocopheryl ether, 0.1-1 part of hydrogenated lecithin, 3-12 parts of skin-moistening grease, 3-10 parts of humectant, 0.0001-0.01 part of red scorpion toxin peptide, 0.1-3 parts of ectoine, 0.05-2 parts of extract of dying of the grass, 0.01-2 parts of ergothioneine, 0.001-0.1 part of acetyl hexapeptide, 0.001-0.1 part of dipeptide diamino Ding Xianbian-base amide diacetate, 0.1-2 parts of hydrolyzed collagen and 50-85 parts of deionized water. According to the invention, through the synergistic effect of multiple anti-wrinkle tightening efficacy components, instant anti-wrinkle and long-acting anti-wrinkle are combined, multiple effective moisturizing components are used for deeply moisturizing and preserving water on the surface layer for a long time, so that the effects of improving tension and skin texture, brightening skin and moisturizing are achieved.

Description

Anti-wrinkle compact micro-nano essence emulsion and preparation method thereof

Technical Field

The invention belongs to the field of skin care products, and particularly relates to anti-wrinkle tightening micro-nano essence emulsion and a preparation method thereof, and more particularly relates to anti-wrinkle tightening micro-nano essence emulsion containing ginseng tissue culture and a preparation method thereof.

Background

The physiological age gradually increases, so that the cell metabolism of the skin is gradually slowed down and the cell aging speed is gradually increased due to the influence of external pressure, environmental factors and the like. Further, the deep wrinkles, expression lines and skin sagging phenomenon on the skin surface become more serious year by year with the loss of collagen, elastin and hyaluronic acid in cells. Endogenous aging of skin is one of physiological characteristics of metabolism of human body, and is an irreversible natural process. Exogenous aging refers to aging process of human body under the influence of external environmental stimulus and long-term accumulation, such as ultraviolet irradiation, pollution, smoking, drinking, long-term nutritional deficiency, pressure, etc.

With the increasing demands for quality of life, people are increasingly conscious of skin protection. For example, by enhancing skin care, cleansing, moisturization and sunscreening of the skin are made; and then, a plurality of skin care products which are capable of tightening, resisting wrinkle and oxidation and brightening skin are matched, so that the purposes of tightening skin, relieving skin wrinkles and brightening skin are expected to be achieved, and the skin aging speed is further slowed down.

Elastase has the ability to degrade various proteins such as collagen and elastin. Changes in elastin structure during skin aging are one of the important causes of wrinkles and skin laxity, and inhibition of elastase to break down elastin can play an anti-aging and anti-wrinkle role. Normal physiological metabolism and environmental influence of human body, such as ultraviolet irradiation, environmental pollution, etc., can also cause generation of Reactive Oxygen Species (ROS), and excessive free radicals can cause skin aging. Reducing free radical production and scavenging aging metabolites is also one of the methods of slowing down skin aging and wrinkle production.

Most of the anti-wrinkle components added in the existing anti-wrinkle compact skin care products are simply compounded, the action mechanism is single, the effects cannot be systematically exerted, and the synergistic effect cannot be achieved, so that the anti-wrinkle effect is general, the time period required for generating the effect is long, the effect is slow, or part of the products have short-term anti-wrinkle effects, but the duration is short, and the long-term anti-wrinkle effect is not achieved; namely, the effects of instant wrinkle resistance and long-acting wrinkle resistance cannot be simultaneously realized.

The present invention has been made in view of this.

Disclosure of Invention

The technical problem to be solved by the invention is to overcome the defects of the prior art, and provide the micro-nano essence emulsion and the preparation method thereof, wherein the micro-nano essence emulsion contains the ginseng tissue culture, and various active ingredients in the micro-nano essence emulsion act synergistically, so that the skin wrinkles can be quickly improved in a short time, and the effects of improving the skin wrinkles, brightening the skin and moisturizing the skin can be achieved by combining the effects of tightening and immediately and long-acting wrinkle resistance.

In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that:

the invention provides micro-nano essence emulsion containing ginseng tissue culture, which comprises the following components in parts by weight:

0.005-0.1 part of ginseng tissue culture, 0.1-5 parts of PEG/PPG-70/30 tocopheryl ether, 0.1-1 part of hydrogenated lecithin, 3-12 parts of skin-moistening grease, 3-15 parts of humectant, 0.0001-0.01 part of red scorpion toxin peptide, 0.1-3 parts of ectoine, 0.05-2 parts of extract of dying of the grass, 0.01-2 parts of ergothioneine, 0.001-0.1 part of acetyl hexapeptide, 0.001-0.1 part of dipeptide diamino Ding Xianbian-base amide diacetate, 0.1-2 parts of hydrolyzed collagen and 50-85 parts of deionized water.

In the micro-nano essence emulsion, PEG/PPG-70/30 tocopheryl ether is adopted to match hydrogenated lecithin, and a conventional emulsification method is adopted to obtain the structure of the micro-nano emulsion. Compared with the conventional common emulsion, the micro-nano emulsion has smaller liquid drop structure and better stability, and is more beneficial to long-term stable storage of skin care products; meanwhile, the smaller the particle size of the liquid drops, the easier the nutrient substances are absorbed by the skin, and the absorption speed is higher. The preparation of the micro-nano emulsion generally requires high equipment and complex processes, and a common normal-pressure emulsification method, such as a homogenizer, can only prepare the conventional common emulsion. According to the invention, PEG/PPG-70/30 tocopheryl ether is matched with hydrogenated lecithin, and a normal pressure emulsification method is adopted to obtain the micro-nano emulsion structure.

Wherein, the PEG/PPG-70/30 tocopherol ether has an aromatic ring (VE) structure, has a heterocycle and a branched chain, can be better dissolved with cosmetic grease, and has smaller and more stable emulsified particles than the conventional emulsifier. Hydrogenated lecithin is a stable emulsifier formed by hydrogenation of lecithin under the action of a catalyst, is white free-flowing powder, has no peculiar smell, is a novel emulsifier, and well retains the active ingredients of the lecithin. The cosmetic has strong hydrophilicity and moisture retention, has strong affinity to skin and mucous membrane, and can play roles of moisture retention, emulsification, dispersion, antioxidation and the like when used in the formulation of cosmetics; the two components complement each other, and the skin-moistening grease is emulsified together to form a micro-nano emulsion structure through the synergistic effect.

Further versions, PEG/PPG-70/30 tocopheryl ether: the mass ratio of the hydrogenated lecithin is 1 (0.1-2),

preferably, PEG/PPG-70/30 tocopheryl ether: the mass ratio of the hydrogenated lecithin is 1:0.5.

When PEG/PPG-70/30 tocopheryl ether: when the mass ratio of the hydrogenated lecithin is 1 (0.1-2), the synergistic effect of the hydrogenated lecithin and the nano-microemulsion is good, the particle size distribution interval of the prepared nano-microemulsion is proper, and when the mass ratio of the hydrogenated lecithin to the nano-microemulsion is 1:0.5, the effect is optimal. If the auxiliary emulsifier is too high when the hydrogenated lecithin is too much, the HLB value is too high, the emulsification effect is poor, and the particle size of the liquid drops is too large; if the amount of hydrogenated lecithin is too small, the co-emulsifier is small, and the effect of synergistically increasing and reducing the particle size of the liquid droplets cannot be achieved.

Further, the particle size distribution interval of the obtained nano-microemulsion is as follows: d50:100-300nm and D90:350-500nm. Thus, the prepared nano microemulsion has small particle size, good stability, easier absorption of nutrient substances by skin, faster absorption speed of emulsion and more fresh skin feel

Further scheme, the mass ratio of the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian-base amide diacetate is 1: (5-10): (2-5);

preferably, the mass ratio of the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian-base amide diacetate is 1:8:3.

the micro-nano essence emulsion adopts three types of instant anti-wrinkle polypeptides with different action mechanisms, namely the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian-based amide diacetate, and the three types of instant anti-wrinkle polypeptides cooperate to act cooperatively, wherein the red scorpion toxin is mainly used for effectively relaxing facial muscles, and the facial expression is more natural while resisting wrinkles in real time; acetyl hexapeptide-8 acts on muscle contraction signaling to relieve muscle contraction force; dipeptide diamino Ding Xianbian-based amide diacetate mainly acts to tighten the superficial skin immediately; the facial muscles are relaxed, the muscle contraction force is reduced, the surface skin state is simultaneously tightened, and the facial muscles and the surface skin state are simultaneously performed, so that a better instant anti-wrinkle effect can be achieved. The erythrosin peptide is a biological simulation conotoxin peptide, and provides a very strong muscle relaxation effect through sequencing and artificial synthesis, and provides a rapid and instant anti-wrinkle effect. The red scorpion toxin provides effective muscle relaxation but not complete stiffness paralysis, and keeps 5% of neuromuscular current conduction of the muscle, so that a natural rejuvenating effect is provided. The red scorpion toxin is a highly folded peptide, is strong in penetration and high in stability, and aims at achieving the effect of relaxing muscles. Acetyl hexapeptide-8 is a neurotransmitter-inhibiting peptide suitable for reducing wrinkles caused by the contraction of expressive muscles, reducing the force of the contraction of facial muscles, and relaxing facial muscles, especially the expressive muscles close to eyes, forehead and the like, similar to the action of botulinum. Dipeptide diamino Ding Xianbian-yl amid diacetate is a synthetic peptide simulating snake venom serum, and can prevent nerve conduction of acetylcholine, and make loose skin compact and elastic, thereby improving dynamic wrinkles.

The invention discovers that when the mass ratio of the red scorpion toxin peptide to the acetyl hexapeptide-8 to the dipeptide diamino Ding Xianbian base amide diacetate is 1: (5-10): in the step (2-5), the synergistic effect of three instant anti-wrinkle peptides is better, the instant anti-wrinkle effect is better, and the mass ratio is 1:8:3, the effect is optimal.

In a further scheme, the mass ratio of the ginseng tissue culture to the hydrolyzed collagen to the ergothioneine to the ectoin to the extract of the dead grass is 1 (5-20): (5-20): (10-30): (10-30),

preferably, the mass ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoin, and the extract of dying grass is 1:15:15:20:25.

In the anti-wrinkle compact micro-nano essence emulsion, the ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and the extract of the dead grass are synergistic, multiple anti-wrinkle compact functional components are synergistic, the ginseng tissue culture can deeply supplement and promote the synthesis of collagen and elastin, the ergothioneine has very excellent effects of resisting oxidation and scavenging free radicals, and the ectoine and the extract of the dead grass have the effects of protecting the activity of epidermal cells, increasing the activity of skin cells and promoting the metabolism of skin cells; further, 5 anti-wrinkle tightening raw materials simultaneously act from multiple aspects so as to achieve better long-acting anti-wrinkle tightening effect. The ginseng is used for beautifying and protecting skin, and can not only improve the antioxidant enzyme activity of skin, increase the content of collagen, inhibit the activity of metalloproteinase MMP-1, slow down the loss of collagen and reduce skin wrinkles, but also protect keratinocytes and dermal fibroblasts from damage caused by ultraviolet rays. Ergothioneine is a natural amino acid which naturally exists in animals and plants and has rich content, can not be synthesized by animal organisms and can only be taken from food, and belongs to rare amino acid; ergothioneine plays an important role in the synthesis process of organism cells and has strong oxidation resistance; meanwhile, the cell physiological protective agent has various physiological functions of scavenging free radicals, resisting aging and radiation, maintaining normal growth of cells and the like. The ectoin is derived from highly halophilous bacteria, and thus is referred to as "salt tolerant bacteria extract". Under extreme conditions of high salt, high temperature, and high ultraviolet radiation, escitalopram protects halophilic bacteria from injury. Research results show that the ectoin also has good repairing protection, anti-aging, moisturizing and sun-screening effects on skin. Hydrolyzed collagen is also known as collagen peptide or collagen powder, not just protein powder: it is rich in amino acids required by human body, and can construct and repair skin cells, supplement all amino acids required by skin collagen synthesis, and achieve the purpose of increasing skin moisture and skin elasticity. The extract of the dying grass has the main component of glyceroglycosides through foreign scientific researches, can protect cell membranes and cell structure stability, has excellent water locking capability similar to that of hyaluronic acid, and excites cell activity from multiple aspects of metabolism, antioxidation, collagen synthesis and the like.

Further, the anti-wrinkle compact micro-nano essence emulsion also comprises the following components in parts by weight:

0.001-0.1 part of palmitoyl tetrapeptide-7, 0.001-0.1 part of palmitoyl pentapeptide-4, 0.001-0.1 part of palmitoyl tripeptide-5, and 0.001-0.1 part of hexapeptide-9.

The anti-wrinkle compact micro-nano essence emulsion also comprises a plurality of peptides, so that the protein synthesis is further promoted or the collagen degradation is inhibited, the anti-wrinkle compact effect is further improved, the long-term anti-wrinkle effect is ensured, and the skin is improved.

In a further scheme, the anti-wrinkle tightening micro-nano essence emulsion comprises the following components in parts by weight: 0.5-5 parts of squalane, 1-5 parts of isononyl isononanoate, 1-5 parts of polydimethylsiloxane and 0.01-1 part of ubiquinone;

the humectant comprises the following components in parts by weight:

0.5-3 parts of betaine, 0.1-2 parts of panthenol, 0.1-3 parts of chitosan amine, 0.01-1 part of sodium hyaluronate, 0.01-1 part of hydrolyzed sodium hyaluronate, 1-5 parts of 1, 2-pentanediol, 2-6 parts of butanediol and 0.1-3 parts of trehalose.

The micro-nano essence emulsion also comprises various lubricating, moisturizing and antioxidant components, promotes the expression of aquaporin, deeply supplements water, and surface layer moisturizing, and enhances the moisturizing capability of skin cells.

That is, the anti-wrinkle compact micro-nano essence emulsion containing the ginseng tissue culture of the invention comprises the following components in parts by weight:

0.005-0.1 part of ginseng tissue culture, 0.1-5 parts of PEG/PPG-70/30 tocopheryl ether, 0.0001-0.01 part of red scorpion toxin peptide, 0.5-5 parts of squalane, 0.5-3 parts of betaine, 0.1-3 parts of ectoin, 0.1-2 parts of panthenol, 0.1-3 parts of acetylglucosamine, 0.05-2 parts of a dieback herb extract, 0.1-1 part of hydrogenated lecithin, 0.01-1 part of sodium hyaluronate, 0.01-2 parts of ergothionein, 0.01-1 part of hydrolyzed sodium hyaluronate, 1, 2-pentanediol, 2-6 parts of butanediol, 1-5 parts of isononyl isononanoate, 0.1-3 parts of trehalose, 1-5 parts of polydimethylsiloxane, 0.001-0.1 part of palmitoyl tetrapeptide, 0.001-0.1 part of palmitoyl pentapeptide, 0.001-0.1 part of palmitoyl tripeptide-5.001-1 part of palmitoyl tripeptide-9.001-1 part of hexaquinone, 0.001-1 part of diacetyl hexa-9.001-1, 0.1 part of diacetyl hexa-0.1-0.001-0.8 part of diacetyl amide, and 1-0.85 part of deionized water.

As a preferred embodiment, the anti-wrinkle compact micro-nano essence emulsion containing ginseng tissue culture comprises the following components in parts by weight:

0.05 part of ginseng tissue culture, 0.8 part of PEG/PPG-70/30 tocopheryl ether, 0.01 part of red scorpion toxin peptide, 3 parts of squalane, 3 parts of betaine, 1 part of ectoin, 1 part of panthenol, 2 parts of acetylglucosamine, 1.25 parts of a common sage herb extract, 0.4 part of hydrogenated lecithin, 0.5 part of sodium hyaluronate, 0.75 part of ergothionein, 0.5 part of hydrolyzed sodium hyaluronate, 4 parts of 1, 2-pentanediol, 4 parts of butanediol, 3 parts of isononyl isononanoate, 1 part of trehalose, 3 parts of polydimethylsiloxane, 0.1 part of palmitoyl tetrapeptide-7, 0.1 part of palmitoyl pentapeptide-4, 0.1 part of palmitoyl tripeptide-5, 0.1 part of hexapeptide-9, 0.08 part of acetyl hexapeptide-8, 0.03 part of dipeptide diamino Ding Xianbian-diamide diacetate, 0.75 part of hydrolyzed collagen, 0.05 part of ubiquinone, and 100 parts of deionized water.

When the weight parts of the components are in the proportion, the skin texture and wrinkle improvement effect is better, and the tightening effect is better.

In addition, in the invention, the ginseng tissue culture is tissue cultured ginseng adventitious roots, and can be obtained by culturing by adopting a method in the prior art. As a preferred mode, the ginseng tissue culture in the invention can adopt the method for culturing adventitious roots of ginseng in the patent of CN 112931216, CN112806267 and CN 113186150 which are issued by the applicant, and the invention is not repeated. Then placing the adventitious roots of the ginseng obtained by tissue culture into a blast drier at 40 ℃ for drying for 4-8 hours, heating to 45-50 ℃ and continuously drying for 5-10 hours; then cooling to 35-40 ℃, drying for 10-15h by low-temperature air blast until the moisture content is less than 10%, stopping drying, and cooling to room temperature. The medium-low temperature interval mode is adopted to carry out air blast or air circulation mode to carry out drying, so that the color change of the adventitious roots of the ginseng and the loss of nutritional ingredients can be prevented to the greatest extent. Pulverizing dried adventitious root of Ginseng radix with superfine pulverizer, sieving to obtain 200-400 mesh tissue culture of Ginseng radix, wherein the content of ginsenoside is high, and the content of rare ginsenoside is high.

The micro-nano essence emulsion containing the ginseng tissue culture provided by the invention is added with multiple effective moisturizing components, so that the skin moisturizing capability can be enhanced, and meanwhile, the micro-nano essence emulsion has a certain effect of brightening the skin, such as a dying grass extract, acetylglucosamine, hyaluronic acid, panthenol and the like, and can promote the expression of aquaporin, deeply moisturize, and moisturize the surface layer, and enhance the skin cell moisturizing capability.

In the invention, the elastase inhibition rate of the micro-nano essence emulsion diluted to 50% is 10-30%, and the clearance rate of DPPH free radical is 20-40%; further proves that the product has better tightening and anti-wrinkle effects.

The invention also provides a preparation method of the micro-nano essence emulsion containing the ginseng tissue culture, which comprises the following steps:

1 aqueous phase: adding humectant and extract of herba Cynanchi Stauntonii into 90% part of deionized water, heating to 80-85deg.C, and stirring to disperse completely;

s2 oil phase: stirring PEG/PPG-70/30 tocopheryl ether, hydrogenated lecithin and skin-moistening oil, heating to melt, dispersing uniformly, heating the oil phase to 80-85deg.C, and maintaining;

s3: mixing the oil phase and the water phase, starting homogenizing and stirring for 10-15 minutes, and maintaining the temperature at 80-85 ℃;

s4: after homogenization, cooling to 45-40 ℃, adding ginseng tissue culture, red scorpion toxin peptide, ectoin, ergothioneine, acetyl hexapeptide-8, dipeptide diamino Ding Xianbian-yl amide diacetate, uniformly stirring hydrolyzed collagen, and supplementing the rest 10% deionized water to 100 parts.

Further, the preparation method comprises the following steps:

s1, water phase: adding butanediol, pentanediol, trehalose, betaine, panthenol, chitosan, extract of Saurus chinensis, sodium hyaluronate and hydrolyzed sodium hyaluronate into 90% deionized water, heating to 80-85deg.C, and stirring to completely disperse;

s2 oil phase: stirring, heating, melting and dispersing squalane, PEG/PPG-70/30 tocopherol ether, polydimethylsiloxane, isononyl isononanoate, hydrogenated lecithin and ubiquinone uniformly, and heating the oil phase to 80-85 ℃ for maintaining;

s4: after homogenization, cooling to 45-40 ℃, adding the ginseng tissue culture, the red scorpion toxin peptide, the ectoin, the ergothioneine, the acetyl hexapeptide-8, the dipeptide diamino Ding Xianbian-base amide diacetate, hydrolyzing collagen, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, palmitoyl tripeptide-5 and hexapeptide-9, and uniformly stirring to supplement the rest 10 percent of deionized water to 100 parts.

After the technical scheme is adopted, compared with the prior art, the invention has the following beneficial effects:

1. the preparation formulation adopts the texture of the micro-nano emulsion, PEG/PPG-70/30 tocopheryl ether is added to match hydrogenated lecithin, and the structure of the micro-nano emulsion can be obtained by adopting an atmospheric emulsification method. The PEG/PPG-70/30 tocopherol ether has an aromatic ring (VE) structure, has a heterocycle and a branched chain, can be better dissolved with cosmetic grease, and has smaller and more stable emulsified particles than the conventional emulsifier. The two components complement each other, and the skin-moistening grease is emulsified together to form a micro-nano emulsion structure through a synergistic effect, and the particle size distribution interval of the obtained nano-micro emulsion is as follows: d50:100-300nm and D90:350-500nm. Therefore, the adopted equipment is simple, the operation is simple, the cost is low, the stability of the micro-nano emulsion is high, the functional components are easier to absorb, and the good tightening and wrinkle removing effects are maintained.

2. In the anti-wrinkle compact micro-nano essence emulsion, three types of instant anti-wrinkle polypeptides with different action mechanisms, namely, the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian-based amide diacetate, can be synergistically enhanced under a certain mass ratio, achieve the effect of 1+1+1 & gt3, and can achieve a better instant anti-wrinkle effect.

3. In the anti-wrinkle compact micro-nano essence emulsion, the added ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoin and the extract of the dead grass generate a synergistic effect under a certain mass ratio, and multiple anti-wrinkle compact functional components deeply supplement and promote the synthesis of collagen and elastin from different layers, resist oxidation, protect the activity of epidermal cells, promote the activity of cells and promote the metabolism of skin cells so as to achieve better long-acting anti-wrinkle and compact effects.

4. The anti-wrinkle compact micro-nano essence emulsion further comprises various peptides, so that the protein synthesis is further promoted or the collagen degradation is inhibited, the anti-wrinkle compact effect is further improved, the long-term anti-wrinkle effect is ensured, and the skin is improved; and various lubricating, moisturizing and antioxidant components, can promote the expression of aquaporin, deeply moisturize, and surface layer moisturize, and enhance the moisturizing capability of skin cells.

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a schematic diagram of particle size distribution of the micro-nano emulsion of the present invention.

It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

The tissue culture of ginseng in the examples or test examples of the present invention was prepared using adventitious roots of ginseng prepared by the method of example 24 in the applicant's patent publication No. CN 112806267. Placing the adventitious roots of the ginseng into a blast drier at 40 ℃, drying for 5 hours, heating to 45 ℃, and continuously drying for 6 hours; then cooling to 35 ℃, drying by low-temperature air blast until the moisture content is less than 10%, stopping drying, and cooling to room temperature. Then pulverizing by a superfine pulverizer, sieving to obtain 200-400 mesh ginseng tissue culture.

The use of the ginseng adventitious roots described above is only one specific embodiment, and is illustrative and not intended to limit the scope of the present application.

Example 1

The micro-nano essence emulsion containing the ginseng tissue culture comprises the following components in parts by weight: 0.02 part of ginseng tissue culture, 0.8 part of PEG/PPG-70/30 tocopheryl ether, 0.005 part of red scorpion toxin peptide, 2 parts of squalane, 2 parts of betaine, 0.3 part of ectoin, 0.5 part of panthenol, 1 part of acetylglucosamine, 0.4 part of a dying grass extract, 0.4 part of hydrogenated lecithin, 0.15 part of sodium hyaluronate, 0.2 part of ergothionein, 0.05 part of hydrolyzed sodium hyaluronate, 2 parts of 1, 2-pentanediol, 2 parts of butanediol, 2 parts of isononyl isononanoate, 0.5 part of trehalose, 3 parts of polydimethylsiloxane, 0.02 part of palmitoyl tetrapeptide-7, 0.02 part of palmitoyl pentapeptide-4, 0.02 part of palmitoyl tripeptide-5, 0.02 part of hexapeptide-9.02 part of acetyl hexapeptide-8.03 part of dipeptide diamino Ding Xianbian-diamide diacetate, 0.2 part of hydrolyzed collagen, and 0.01 part of ubiquinone, wherein the total amount is 100 parts of deionized water.

The preparation method comprises the following steps:

s1, water phase: adding butanediol, pentanediol, trehalose, betaine, panthenol, chitosan, extract of Saurus chinensis, sodium hyaluronate and hydrolyzed sodium hyaluronate into 90% deionized water, heating to 85deg.C, and stirring to completely disperse.

S2 oil phase: the squalane, PEG/PPG-70/30 tocopheryl ether, polydimethylsiloxane, isononyl isononanoate, hydrogenated lecithin and ubiquinone are stirred, heated, melted, dispersed uniformly, and the oil phase is heated to 85 ℃ for maintenance.

S3: mixing the oil phase and the water phase, stirring homogeneously for 10-15min, and maintaining the temperature at 80-85 ℃.

Example 2

The micro-nano essence emulsion containing the ginseng tissue culture comprises the following components in parts by weight: 0.05 part of ginseng tissue culture, 0.8 part of PEG/PPG-70/30 tocopheryl ether, 0.01 part of red scorpion toxin peptide, 3 parts of squalane, 3 parts of betaine, 1 part of ectoin, 1 part of panthenol, 2 parts of acetylglucosamine, 1.25 parts of a common sage herb extract, 0.4 part of hydrogenated lecithin, 0.5 part of sodium hyaluronate, 0.75 part of ergothionein, 0.5 part of hydrolyzed sodium hyaluronate, 4 parts of 1, 2-pentanediol, 4 parts of butanediol, 3 parts of isononyl isononanoate, 1 part of trehalose, 3 parts of polydimethylsiloxane, 0.1 part of palmitoyl tetrapeptide-7, 40.1 parts of palmitoyl pentapeptide-5.1 parts of palmitoyl tripeptide-9, 0.1 part of hexapeptide-8.08 parts of acetyl hexapeptide-0.03 part of dipeptide diamino Ding Xianbian base amide diacetate, 0.75 part of hydrolyzed collagen, 0.05 part of quinone and 100 parts of deionized water.

The preparation method comprises the following steps: the preparation method is the same as that of example 1, and will not be described again.

Example 3

The micro-nano essence emulsion containing the ginseng tissue culture comprises the following components in parts by weight: 0.05 part of ginseng tissue culture, 0.8 part of PEG/PPG-70/30 tocopheryl ether, 0.01 part of red scorpion toxin peptide, 3 parts of squalane, 2.5 parts of betaine, 1.5 parts of ectoin, 2 parts of panthenol, 3 parts of acetylglucosamine, 1.5 parts of a dying grass extract, 0.4 part of hydrogenated lecithin, 1 part of sodium hyaluronate, 1 part of ergothionein, 1 part of hydrolyzed sodium hyaluronate, 3 parts of 1, 2-pentanediol, 5 parts of butanediol, 3 parts of isononyl isononanoate, 3 parts of trehalose, 3 parts of polydimethylsiloxane, 0.06 part of palmitoyl tetrapeptide-7, 0.06 part of palmitoyl pentapeptide-4, 0.06 part of palmitoyl tripeptide-5, 0.06 part of hexapeptide-9, 0.1 part of acetyl hexapeptide-8, 0.05 part of dipeptidyl diamide diacetate, 1 part of hydrolyzed collagen, 0.06 part of ubiquinone, and 100 parts of deionized water.

The micro-nano essence emulsion effect of the tissue culture containing ginseng of the present invention was analyzed by the following test.

Experimental effect example 1: the effect of the micro-nano essence emulsion for improving skin texture is detected

30 healthy experimenters 25-55 years old are selected, the average number is divided into 3 groups, experiments are carried out after training by professionals, the experimenters have no skin medical history, products provided by professionals are needed during the test, and no medicines or cosmetics irrelevant to the experiments are smeared. Before the product is used, the face is cleaned by the same facial cleanser, then the face is wiped dry, and the initial value of the skin texture before the use is photographed and analyzed by a VISIA detector. Then the facial mask is used once in the morning and evening every day, and the face is cleaned before the essence cream is used, and the same type of moisturizing water is used; the same type of moisturizing cream is used after the essence cream is used. According to the prescribed use method, after one month of trial, the face is cleaned by using the facial cleanser, and after the face is wiped dry, the skin texture value after use is photographed and analyzed by using the VISIA detector. The change condition of the skin texture before and after the use of the mask is obtained through the comparison analysis.

Group 1 was tested using the cream from example 1 to obtain the data of table 1; group 2 the extract milk obtained in example 2 was selected for testing to obtain the data of table 2; the 3 groups were tested using the cream from example 3, and the data in table 3 were obtained.

Under the same condition, all experimental data of the experiment are the quantity of the skin texture features measured by the VISIA tester after photographing and analyzing, and the smaller the numerical value is, the better the numerical value is, and the smaller the numerical value is, the smoother and finer the skin surface is.

Table 1: front and rear skin texture characteristic quantity values using the essence milk prepared in example 1

Experiential player	Initial value	Test value	Change value
				No. 1	2420	1928	-492
No. 2	2162	1725	-437
				No. 3	1028	824	-204
No. 4	3012	2769	-243
				No. 5	1967	1427	-540
No. 6	2264	1978	-286
				No. 7	1845	1726	-119
No. 8	2009	1746	-263
				No. 9	3054	2679	-375
No. 10	1649	1426	-223

Table 2: front and rear skin texture characteristic quantity values using the essence milk prepared in example 2

Table 3: front and rear skin texture characteristic quantity values using the essence milk prepared in example 3

Experiential player	Initial value	Test value	Change value
				No. 21	2185	1796	-389
No. 22	1876	1354	-522
				No. 23	2598	2177	-421
No. 24	1888	1499	-389
				25 #	2471	2011	-460
No. 26	3276	2596	-680
				No. 27	1968	1642	-326
No. 28	1856	1411	-445
				29 # 29	2019	1644	-375
No. 30	1866	1531	-335

As can be seen from the results of table 1, table 2 and table 3, the test values of the skin texture feature number are lower than the initial values after the experimenter uses the essence milk of the invention; namely, the skin smoothness is improved to different degrees; the effect of the essence emulsion of the invention on smoothing skin is illustrated.

Experimental Effect example 2 the micro-nano essence emulsion of the invention is detected to have the effects of anti-wrinkle and tightening

Due to the lack of collagen and elastin, skin structure collapse can lead to skin sagging and gradual sagging, and true wrinkles can occur. The VISIA detector can reflect the skin wrinkle condition through photographing analysis. The experiment tests the skin wrinkle change condition before and after the experimenter uses the essence milk. Skin wrinkle test data for this experiment were obtained with a VISIA detector in the united states.

The skin wrinkles before and after the use of the essence emulsion are obtained by comparison analysis according to the test method and the test process of the experimental effect example 1.

Group 1 was tested using the cream from example 1 to obtain the data of table 4; group 2 was tested using the cream from example 2 to obtain the data of table 5; the 3 groups were tested using the cream from example 3, and the data in table 6 were obtained.

Under the same condition, all experimental data of the experiment are the characteristic quantity of the skin wrinkles measured by the VISIA tester after photographing and analyzing, and the smaller the numerical value is, the better the numerical value is, and the smaller the numerical value is, the less the skin wrinkles are.

Table 4: skin wrinkle changes before and after use of the essence emulsion prepared in example 1

Experiential player	Initial value	Test value	Change value
				No. 1	106	98	-8
No. 2	127	104	-23
				No. 3	87	79	-8
No. 4	64	52	-12
				No. 5	113	96	-17
No. 6	110	99	-11
				No. 7	82	75	-7
No. 8	94	86	-8
				No. 9	91	78	-13
No. 10	72	58	-14

Table 5: skin wrinkle changes before and after use of the essence milk prepared in example 2

Experiential player	Initial value	Test value	Change value
				11 # 11	89	44	-45
No. 12	120	88	-32
				No. 13	58	27	-31
No. 14	105	84	-21
				No. 15	118	81	-37
Number 16	149	102	-47
				No. 17	86	52	-34
No. 18	92	62	-30
				19 # 19	129	91	-38
No. 20	67	38	-29

Table 6: skin wrinkle changes before and after use of the essence milk prepared in example 3

As can be seen from the results in table 4, table 5 and table 6, the test values of the characteristic quantity of skin wrinkles are lower than the initial values after the experimenter uses the essence milk of the invention; namely, the skin wrinkles are improved to different degrees; the essence emulsion disclosed by the invention has the effects of improving tension and reducing wrinkles.

According to analysis of experience data in tables 1-6, the effect of improving texture and wrinkles of an experimenter is obviously better than that of the embodiment 3 and the embodiment 1 by using the essence emulsion in the embodiment 2, the use proportion of each component in the embodiment 1 is smaller than that in the embodiment 2, and the use proportion of each component in the embodiment 3 is higher, so that under the combination component proportion of the embodiment 2, the synergistic effect of each component can play a better role in tightening and resisting wrinkles and improving skin textures.

Experimental Effect example 3 detection of the particle size of the essence emulsion of the present invention

The particle size distribution of the essence sample of example 2 was measured as follows:

TABLE 7 micro-nano essence emulsion particle size distribution

As can be seen from Table 7, the particle size D50 of the micro-nano emulsion of the invention is 134nm, D90:277nm; the emulsifier PEG/PPG-70/30 tocopheryl ether is matched with hydrogenated lecithin in a ratio of 1:0.5, and the mixture is homogenized for 10-15min at 80-85 ℃ by a normal pressure emulsification method, so that the micro-nano emulsion with the particle size of about 200nm can be achieved.

Thus, the smaller the particle size of the prepared nano-microemulsion is, the better the stability is, the more easily the nutrient substances are absorbed by the skin, the faster the absorption speed of the emulsion is and the more fresh and cool the skin feel is used.

In addition, referring to the formulation and preparation method of example 2, the mass ratio of PEG/PPG-70/30 tocopheryl ether to hydrogenated lecithin was adjusted, wherein the amount of PEG/PPG-70/30 tocopheryl ether added was 0.8 part, and the amounts of hydrogenated lecithin added were 0.08 part, 0.4 part, 0.8 part, and 1.6 part, respectively, to examine the effect of the mass ratio of PEG/PPG-70/30 tocopheryl ether to hydrogenated lecithin on the particle size of the emulsion. The results are shown in Table 8.

TABLE 8

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Analysis of results: according to the data of the particle size distribution test of the essence milk prepared by emulsifying agents with different mass ratios, the mass ratio of PEG/PPG-70/30 tocopheryl ether to hydrogenated lecithin is 1:0.5, the particle size of the essence milk is minimum, and the D50 can reach about 200 nm. Further, when the hydrogenated lecithin is too much, the emulsification effect is poor, and the particle size of the liquid drops is too large; if the dosage of hydrogenated lecithin is too small, the auxiliary emulsifier is less, and the synergistic effect cannot be achieved, so that the effect of reducing the particle size of liquid drops is not achieved; thus, the PEG/PPG-70/30 tocopheryl ether has a synergistic effect with hydrogenated lecithin.

Experimental effect example 4 the efficacy effect of the essence milk of the present invention was examined by in vitro experiments

The essence emulsion of the invention of example 2 was subjected to an in vitro test for elastase inhibition and DPPH free radical scavenging.

Elastase inhibition assay: diluting the essence emulsion into 50% emulsion by using a buffer solution, uniformly mixing the emulsion with elastase, inoculating for 10min at 25 ℃, and analyzing by measuring a light absorption value by an ultraviolet spectrophotometer to obtain the inhibition rate of the essence emulsion elastase and comparing the inhibition rate with a blank control, wherein the result is as follows:

TABLE 9

DPPH radical scavenging experiments: diluting the essence milk into 50% emulsion by using 95% ethanol, uniformly mixing the emulsion with a DPPH free radical sample, standing for 5min at room temperature, and analyzing by measuring a light absorption value by an ultraviolet spectrophotometer to obtain DPPH free radical clearance data of the essence milk and comparing the data with a blank control, wherein the result is as follows:

table 10

	Blank control	Essence milk sample	P value	Difference significance
					Radical scavenging rate%	1.84±0.16	39.5±0.15	＜0.05	Is remarkable in

And (3) comparing and testing the influence of the component proportion of the essence milk on the efficacy of the product:

comparative example 1:

the skin care composition of this comparative example differs from example 2 in that:

the addition ratio of the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian base amide in the skin care composition is 1:3:2, and the total weight component of the 3 ingredients remains unchanged.

Comparative example 2:

the addition ratio of the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian base amide in the skin care composition is 1:7:6; and the total weight components of the 3 components are kept unchanged.

Comparative example 3:

the addition ratio of the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian base amide in the skin care composition is 1:10:6, the addition amount of the red scorpion toxin peptide is unchanged, and the addition amount of the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian base amide is correspondingly increased, namely the total addition amount of the three raw materials is increased.

Comparative example 4:

the skin care composition comprises ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and a dead grass extract in a ratio of 1:5:5:10:10; and the total weight component of 5 components is kept unchanged.

Comparative example 5:

the skin care composition comprises ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and a dead grass extract in a ratio of 1:8:5:12:12; and the total weight component of 5 components is kept unchanged.

Comparative example 6:

the skin care composition comprises ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and a dead grass extract in a ratio of 1:10:10:15:20, a step of; and the total weight component of 5 components is kept unchanged.

Comparative example 7:

the skin care composition of this comparative example differs from example 3 in that:

the skin care composition comprises ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and a dead grass extract in a ratio of 1:20:15:15:10; and the total weight component of 5 components is kept unchanged.

Comparative example 8:

the skin care composition comprises ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and a dead grass extract in a ratio of 1:20:20:25:30; wherein the dosage of the ginseng tissue culture is unchanged, and the other 4 types of components are correspondingly increased in proportion, namely the total anti-wrinkle raw material is increased.

Comparison test result 1:

example 2 and comparative examples 1-3 each collected 10 persons for product experience testing, and after face cleaning with the same type of facial cleanser before use of the product, the face was rubbed dry, and the initial values of skin wrinkles before use were photographed and analyzed using a VISIA detector. Then the skin wrinkle after use is tested again after the same moisturizing water with only basic moisturizing effect, an essence milk sample and the same moisturizing cream with only basic moisturizing effect are smeared for 1 hour according to the following using method, and the moisturizing cream is used once in the morning and evening; instant anti-wrinkle effect of the essence emulsions of comparative example 2 and comparative examples 1 to 3. Each group of values takes the average value of 10 experimenter characteristic values (the smaller the value is, the better); and further obtaining the comparison result of the improvement effect of the product on the skin wrinkles.

Table 11 example 2 and comparative examples 1-3 experience test results

Average value of wrinkles	Initial value	Test value	Change value
				Example 2	99.1	69.8	-29.3
Comparative example 1	92.2	81.8	-10.4
				Comparative example 2	88.9	71.5	-17.4
Comparative example 3	99.8	79	-20.8

As can be seen from the results in Table 8, the experimenters were lower than the initial values in the skin wrinkle characteristic quantity test values after using the samples of example 2 and comparative examples 1 to 3; and the improvement effect of the example 2 is higher than that of the comparative examples 1-3; the three instant anti-wrinkle components have obviously different anti-wrinkle effects in different proportions, and the instant anti-wrinkle effect is optimal under the condition that the proportion of the red scorpion toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian-based amide diacetate is 1:8:3 in the embodiment 2 of the invention. Comparative analysis of comparative examples 1-2 and example 2 shows that the best results were obtained with the optimum ratio, with the total amount of anti-wrinkle peptide used kept the same. Even though the overall use of instant anti-wrinkle peptide of comparative example 3 was increased, the use effect was still not as good as example 2 in the case of non-optimal addition ratio; therefore, the erythropolis toxin peptide, the acetyl hexapeptide-8 and the dipeptide diamino Ding Xianbian-base amide diacetate have obvious synergistic effect.

Comparison test result 2:

10 persons are respectively recruited for product experience test in the example 2 and the comparative examples 4-8, and the test is carried out according to the method of the test example 2; the improvement effect on skin wrinkles is compared with the following result: (Each group of values is an average of 10 experienter characteristic values, the smaller the value, the better the value)

Table 12 example 2 and comparative examples 4-8 experience test results

As can be seen from the results in Table 9, the experimenters were lower than the initial values in the skin wrinkle characteristic quantity test values after using the samples of example 2 and comparative examples 4 to 8; and the improvement effect of the example 2 is higher than that of the comparative examples 4-8; the 5 long-acting anti-wrinkle components have obviously different anti-wrinkle effects at different ratios, and the instant anti-wrinkle effect is optimal under the conditions that the ratio of the ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoin and the dying grass extract is 1:15:15:20:25 in the embodiment 2 of the invention. Comparative analysis of comparative examples 4 to 7 and example 2 revealed that the best effect was obtained under the optimum ratio condition when the total amount of the anti-wrinkle tightening ingredient was the same. Even if the total amount of the anti-wrinkle tightening ingredient of comparative example 8 was increased, the effect of use was still not as good as that of example 2 in the case of non-optimal addition ratio; therefore, the combination of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine and the extract of the dying weed in the essence milk has obvious synergic effect.

The foregoing description is only illustrative of the preferred embodiment of the present invention, and is not to be construed as limiting the invention, but is to be construed as limiting the invention to any simple modification, equivalent variation and variation of the above embodiments according to the technical matter of the present invention without departing from the scope of the invention.

Claims

1. An anti-wrinkle compact micro-nano essence emulsion is characterized in that: comprises the following components in parts by weight:

2. The anti-wrinkle tightening micro-nano-emulsion according to claim 1, wherein PEG/PPG-70/30 tocopheryl ether: the mass ratio of the hydrogenated lecithin is 1 (0.1-2);

3. The anti-wrinkle compact micro-nano essence emulsion according to claim 1 or 2, wherein the particle size distribution interval of the nano-micro emulsion is: d50:100-300nm and D90:350-500nm.

4. The anti-wrinkle tightening micro-nano essence emulsion according to any one of claims 1-3, wherein: the mass ratio of the red scorpion toxin peptide to the acetyl hexapeptide-8 to the dipeptide diamino Ding Xianbian base amide diacetate is 1: (5-10): (2-5);

5. the anti-wrinkle compact micro-nano essence emulsion according to any one of claims 1-4, wherein the mass ratio of ginseng tissue culture, hydrolyzed collagen, ergothioneine, ectoine, and dying weed extract is 1 (5-20): (5-20): (10-30): (10-30),

6. The anti-wrinkle compact micro-nano essence emulsion according to any one of claims 1-5, further comprising the following components in parts by weight:

7. The anti-wrinkle tightening micro-nano essence emulsion according to any one of claims 1-6, wherein the skin-moistening grease comprises the following components in parts by weight:

0.5-5 parts of squalane, 1-5 parts of isononyl isononanoate, 1-5 parts of polydimethylsiloxane and 0.01-1 part of ubiquinone.

The humectant comprises the following components in parts by weight: 0.5-3 parts of betaine, 0.1-2 parts of panthenol, 0.1-3 parts of chitosan amine, 0.01-1 part of sodium hyaluronate, 0.01-1 part of hydrolyzed sodium hyaluronate, 1-5 parts of 1, 2-pentanediol, 2-6 parts of butanediol and 0.1-3 parts of trehalose.

8. The wrinkle resistant compact micro-nano essence emulsion according to any one of claims 1-7, comprising the following components in parts by weight:

9. A method for preparing the anti-wrinkle compact micro-nano essence emulsion according to any one of claims 1-8, comprising the following steps:

s1, water phase: adding humectant and extract of herba Cynanchi Stauntonii into 90% part of deionized water, heating to 80-85deg.C, and stirring to disperse completely;

10. The method of manufacturing according to claim 9, comprising the steps of:

s2 oil phase: stirring, heating, melting and dispersing PEG/PPG-70/30 tocopheryl ether, hydrogenated lecithin, isononyl isononanoate, squalane, polydimethylsiloxane and ubiquinone uniformly, and heating the oil phase to 80-85 ℃ for maintaining;

s4: after homogenization, cooling to 45-40 ℃, adding ginseng tissue culture, red scorpion toxin peptide, ectoin, ergothioneine, acetyl hexapeptide-8, dipeptide diamino Ding Xianbian-yl amide diacetate, hydrolyzing collagen, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, palmitoyl tripeptide-5 and hexapeptide-9, and uniformly stirring to supplement the rest 10% deionized water to 100 parts.