JP5905188B2 - Cosmetic or hair restorer containing sialic acid and EGF and / or FGF and method for producing them - Google Patents

Description

  The present invention relates to a cosmetic and a hair restorer containing sialic acid, EGF and / or FGF, and particularly decomposes sialic acid or the like with a solid acid or a weak acid during the production process.

The troubles about the skin and hair are irrelevant regardless of age, age, or sex, and many improvement measures have been taken so far, such as those that are applied directly to the site and those that are drunk. Some are not seen.
The main reasons are that the human skin condition is not uniform, and hair loss is due to aging, illness, mental causes, and various other reasons. This is because it was difficult to develop a comprehensive response. Moreover, even if it is cosmetics, hair growth agents, hair growth agents, etc., to which a certain degree of action is seen, depending on the ingredients, side effects are seen, and there are many cases where there are defects.
In addition, there is an extremely high interest in beauty regardless of age or gender, and the pursuit of how to enhance the action of cosmetics, especially on the face and skin, and how to improve the safety of the face and skin. The appearance of effective ingredients was awaited.

From this point of view, an attempt was made to develop a revolutionary ingredient that fundamentally reexamines conventional cosmetic compositions, that is, cosmetics, hair growth agents, hair growth agents, and other hair preparations, and the emergence of effective ingredients was awaited. It was.

Needless to say, human skin and scalp are inherently sensitive, and depending on the type of cosmetics, hair restorer, hair growth agent, etc., rashes and itchiness may occur depending on the type of person. It will cause a lot of damage.
Moreover, even if it was an effective component, when the component lacked stabilization, the effect of the component could not be fully utilized.

Patent Document 1 listed below discloses an insulin-like growth factor-1 secretion promoter characterized by containing sialic acid not bound to sugar and / or protein and not containing sericin, The last claim also mentions the hair restorer.

Patent Document 2 discloses a ginsenoside Rb1-like substance screening method for a hair growth composition containing sialic acid.

Patent Document 3 discloses a hair nourishing agent containing sialic acid (including the case of N-acetylneuraminic acid) and a sialic acid derivative.

Patent Document 4 discloses a wound healing agent, a cosmetic and a hair nourishing agent containing lactoferrins, hydrolysates of lactoferrin, or a mixture thereof and an epidermal growth factor.

Patent Documents 5 to 6 disclose cosmetics using sialic acid-containing components, including the case of using an axilla.
However, in the above-mentioned patent documents 1 to 6, there is no disclosure or suggestion about the decomposition of sialic acid by a weak acid of a solid acid as in the present invention, and the features of the present invention, features and effects are not seen This invention is clearly different from the present invention.

JP 2009-073806 A JP 2004-077456 A Japanese Patent No. 2555389 JP-A-5-352423 International Publication No. 99/22709 JP 2003-095961 A

The problem to be solved by the present invention is that sialic acid is contained in axilla, royal jelly, eggs, animal saliva, etc., but is a component such as protein, lipid (fat), sugar chain, etc., forming a polymer doing. In such a state, application to a hair restorer or a cosmetic has a problem of poor absorbability to the scalp and skin. The present invention is a low molecular weight compound that absorbs high-molecular weight conjugated sialic acid, that is, sialic oligosaccharides or sialic acids that are bound to proteins, and polysaccharides that are bound to various monosaccharides, that is, high molecular sialic acid contained in sugar chains. a method of decomposing the sialic acid is a method to leave without breaking together proteinaceous useful substance to coexist (such as epidermal growth factor and line fibroblast growth factor) as much as possible.
Also the conventional example, in order to decompose the sialic acid with an enzyme or an acid, there is a possibility of also cutting useful epidermal growth factor-ray fibroblast growth factor, it has fault respectively. Further, sialic acid is N-acetylneuraminic acid, and there is a high possibility that it will be hydrolyzed and cleaved by strong acid decomposition since an acel group is present in the molecule.
In order to solve these problems, the present inventors have made various studies, and as a result, have found that if the material such as the axilla is treated with a weak acid and / or a solid acid, the object can be achieved, and the present invention has been completed. It was.
Further, the present inventors have by synergy of both joined by epidermal growth factor (EGF) and lines fibroblast growth factor in addition to the hair-growth effect of epidermal growth effect with sialic acid contained in such Tsubame窩(FGF) The present inventors have found that it has an excellent hair growth effect and thus completed the present invention.
Furthermore, an amino acid, a peptide, a polysaccharide or the like known as a natural moisturizing factor is added to provide a cosmetic composition having an excellent moisturizing effect and glossiness.

The above object is achieved according to the present invention, single molecules and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or lines fibroblast growth factor (hereinafter, referred to as FGF) both or these Achieved by a cosmetic or hair restorer, characterized in that it contains all mixtures.

  Further, as the material according to the present invention, preferably, the above-mentioned monomolecular and low molecular sialic acid and EGF and / or FGF are a swallow nest extract (hereinafter referred to as an axilla extract) and / or an animal (in the case of cattle). From the natural product of saliva and / or royal jelly.

Further, the present invention is preferably a Material decomposed by weak acids, using the obtained monomolecular and low molecular sialic acid and EGF and / or FGF.

In the present invention, it is preferable that the weak acid is acetic acid, citric acid, malic acid, or lactic acid.

  In the present invention, it is preferable that the acid decomposition is based on a solid acid.

In the present invention, it is preferable that the solid acid is silica alumina, zeolite, or acidic clay.

  Further, the cosmetic according to the present invention comprises behenyl alcohol, squalane, monolauric acid, if necessary, by adding EGF and / or FGF to EGF and / or FGF derived from single molecule and low molecular weight sialic acid and natural product. Polyoxyethylene sorbitan, macadamia nut oil, sorbitan sesquioleate, glyceryl stearate, tocopherol, glycerin, 1,3-butylene glycol, phenoxyethanol, acrylic acid / methacrylic acid copolymer, potassium hydroxide, iwabenkei root extract, placenta extract , Saitai extract, and one or more components of water-soluble collagen.

  In addition, the hair-growth shampoo according to the present invention may be prepared by adding EGF and / or FGF to EGF and / or FGF derived from the single molecule and low-molecular-weight sialic acid and natural products, if necessary, and adding glycyrrhizic acid 2K, tea extract, Clove extract, mallow extract, yellowfin bark extract, perilla extract, assembly extract, lauroyl hydrolyzed silk Na, hydrolyzed keratin, rice fermentation broth, brown algae extract and / or red algae extract and / or green algae extract, polyquaternium-51 It contains more than seeds.

  Further, the hair growth conditioner according to the present invention further comprises EGF and / or FGF as necessary, in addition to EGF and / or FGF derived from the above-mentioned single molecule and low molecular weight sialic acid and natural products, and glycyrrhizic acid 2K, tea extract, It comprises one or more of the following components: clove extract, mallow extract, yellowfin bark extract, perilla extract, assembly extract, panthenol, rice fermentation broth, and polyquaternium-51.

  In addition, the hair-growth tonic according to the present invention comprises EGF and / or FGF further added to the above-described single molecule and low-molecular sialic acid and natural product-derived EGF and / or FGF, if necessary, to obtain glycyrrhizic acid 2K, assembly extract , Panax ginseng extract, cephalanthin, sugar / amino acid ester-1, placenta extract, menthol, salamander peel extract, rice fermentation broth, gentian root extract, chamomile flower extract, cinnamon flower extract, comfrey leaf extract, arnica flower extract One or more components are blended.

  Further, the above object is achieved by a method for producing a cosmetic or hair restorer characterized by comprising a single molecule and a low molecular sialic acid and EGF and / or FGF, or a mixture of all of them.

  In the production method of the present invention, preferably, the above-mentioned monomolecular and low molecular sialic acid and EGF and / or FGF are axillary extracts and / or natural saliva and / or royal jelly of animals (including cattle). It is derived from things.

The manufacturing method of the present invention, preferably, the Material decomposed by weak acids, using a single molecule and the low molecular sialic acids obtained.

In the production method of the present invention, preferably, the weak acid is acetic acid, citric acid, malic acid, or lactic acid.

  In the production method of the present invention, preferably, the acid decomposition is based on a solid acid.

In the production method of the present invention, preferably, the solid acid is silica alumina, zeolite, or acid clay.

  In addition, the method for producing a cosmetic according to the present invention further comprises adding EGF and / or FGF to EGF and / or FGF derived from mono- and low-molecular-weight sialic acid and natural products as necessary, and then adding behenyl alcohol, squalane. , Polyoxyethylene sorbitan monolaurate, macadamia nut oil, sorbitan sesquioleate, glyceryl stearate, tocopherol, glycerin, 1,3-butylene glycol, phenoxyethanol, acrylic acid / methacrylic acid copolymer, potassium hydroxide, iwabenkei root extract , Placenta extract, saitai extract, and water-soluble collagen.

  In addition, the method for producing a hair-growth shampoo according to the present invention comprises adding EGF and / or FGF to EGF and / or FGF as described above in addition to the above-described monomolecular and low-molecular sialic acid and natural product EGF and / or FGF. , Tea extract, clove extract, mallow extract, yellowfin bark extract, perilla extract, assembly extract, lauroyl hydrolyzed silk Na, hydrolyzed keratin, rice fermentation broth, brown algae extract and / or red algae extract and / or green algae extract, polyquaternium-51 1 or more types of ingredients are blended.

  In addition, the method for producing a hair-growth conditioner according to the present invention comprises adding EGF and / or FGF to EGF and / or FGF derived from the single molecule and low-molecular sialic acid and natural products as necessary, and adding glycyrrhizic acid 2K. , Tea extract, clove extract, mallow extract, yellowfin bark extract, perilla extract, assembly extract, panthenol, rice fermentation broth, and polyquaternium-51.

  In addition, the method for producing a hair-growth tonic according to the present invention further comprises adding EGF and / or FGF to EGF and / or FGF derived from the single molecule and low-molecular-weight sialic acid and natural products as described above, and adding glycyrrhizic acid 2K. , Assembly extract, ginseng extract, cephalanthin, sugar / amino acid ester-1, placenta extract, menthol, salamander peel, rice fermented liquid, gentian root extract, chamomile flower extract, cinnamon flower extract, comfrey leaf extract, arnica flower extract 1 or more types of ingredients are blended.

The cosmetic and hair restorer containing monomolecular and low molecular sialic acid according to the present invention decomposes the axilla containing sialic acid in the molecule with a weak acid such as acetic acid, citric acid, malic acid, and lactic acid.
As described above, EGF-related substances, FGF-related substances, etc. are not decomposed but leave useful proteins and low molecular weight sialic acid, so that they have excellent absorbability to the scalp and skin.

Further, in the present invention, as described above, it is decomposed with a solid acid such as silica alumina, zeolite, acidic clay, etc., so that solid-liquid separation can be easily performed during treatment of axilla and the like containing sialic acid in the molecule. In addition, the use of alkali for neutralization is avoided as much as possible.

Further, according to the present invention, cosmetics and hair restorers containing monomolecular and low molecular sialic acid are used as described above, and solid acids such as silica alumina, zeolite, and acid clay are used for axilla containing sialic acid in the molecule. It has the advantage of obtaining sialic acid which is reduced in molecular weight and has good absorbability to the scalp and skin.

According to the present invention, shampoos, conditioners, and tonics that are more excellent in promoting hair growth can be obtained by the above-described effects, and beauty compositions such as beauty creams that are excellent in moisturizing action and glossiness can be obtained.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The embodiments described below are preferable specific examples of the present invention, and thus various technically preferable limitations are given. However, the scope of the present invention particularly limits the present invention in the following description. As long as there is no description of the effect, it is not restricted to these aspects.

As described above, in the present invention, sialic acid used is derived from natural products such as axillary extract, animal saliva and royal jelly.
In the first place, sialic acid is known to exist in the living body, as a component of complex carbohydrates, as a component of complex carbohydrates, and at the ends of oligosaccharides on the surface of cell membranes and to carry important biological functions. . Among the cell membrane sugar chains, the most important ones are glycolipids and glycoproteins.
Today, it is known that this sialic acid is contained in a large amount in the axilla described below. It is also known to be contained in saliva and royal jelly from animals such as cattle. In the present invention, these natural product-derived components are used, but the sialic acid content (10 to 12%) and a large amount of axilla are particularly used.

The above-mentioned axilla is a swallow {Swallowidae
aliini) is a generic name} that solidifies saliva secreted from the salivary glands developed during the egg-laying stage and builds it up for about one month. The edible food has long been prized in Southeast Asia and China, and is known for its nutrition, beauty and tonic effects. It is also known to protect against infection and produce testosterone (male hormone). This axilla is known to contain an extremely large amount of sialic acid of 10% or more. Compared to humans and bees containing 200 to 1
, 500 times as much sialic acid, and is therefore the best source of sialic acid. In addition, eggs contain 0.2% in dry yolk, 0.02% in milk, and 0.15% in colostrum of milk.

An EGF-related substance is a factor that regenerates cells inherently possessed by humans, and acts on cells on the surface of the skin to promote the formation of new cells. As humans age, the amount of secretion of epidermal growth factor decreases (Life Sciences vol. 31, 679-683, 1982). This causes the skin aging phenomenon.
By supplying this EGF-related substance to the skin, it is possible to promote the formation of fresh skin cells. In other words, it can be said that the aging phenomenon can be reversed. This seems to be the same in the scalp.
Furthermore, FGF-related substance is a protein with a molecular weight of from 16,000 to 20,000 to promote the growth of the line fibroblasts and endothelial cells present in the skin dermis.
The FGF-related substances, so to promote the growth of the line fibroblast cells present in various tissues, its action differentiation of neural cells, ranging variety such as angiogenesis. In addition, the FGF-related substance decreases with age as in the case of the EGF-related substance. Therefore, elasticity and moisturizing skin no longer made of collagen synthesis by line fibroblasts in the dermis is from being diminished.

The action and biological reaction of the axilla used in the present invention are shown below.
In the axilla, fucose, N-acetylneuraminic acid, galactose, mannose, N-
It is known that sugar chains of acetylgalactosamine and N-acetylglucosamine are included.
Among them, N-acetylneuraminic acid related to the present invention is known as sialic acid as described above, and the composition of axillary mucoid is characterized by containing a considerable amount of sialic acid and ash. The
A sialic acid monomolecule prepared by transferase from N-acetylglucosamine and sodium pyruvate stimulates sensory nerves, and it has been confirmed by experiments with mice that this promotes hair growth. (Fragrance Journal, October 2009, page 43) EGF is necessary for the start of the hair growth phase and the transition from the growth phase to the regression phase, and is essential for smooth rotation of the hair cycle, and EGF reception It has also been reported that the body is essential for hair growth. (The Journal of Biological Chemistry, 278, 26120 (2003)) However, the hair growth effect in the situation where both sialic acid and EGF useful for hair growth are mixed is not known. In view of such a situation, the inventors hydrolyzed natural materials such as axilla by the above-mentioned method, found that both of them exist conveniently, and obtained a composition with an excellent hair-growth effect. I found it.

In the present invention, as described above, degradation with weak acids such as acetic acid, citric acid, malic acid, and lactic acid is performed, so that the sialic oligosaccharide becomes sialic acid having a small molecular weight. Moreover, since an EGF related substance and an FGF related substance are not decomposed | disassembled by this, it functions effectively as cosmetics or a hair restorer in combination with sialic acid.
A weak acid refers to an acid having a small acid dissociation constant relative to a strong acid having a large acid dissociation constant.

In the present invention, as described above, since decomposition is performed with a solid acid such as silica alumina, zeolite, and acid clay, solid-liquid separation can be easily performed, and it is not necessary to use an alkali for neutralization. In addition, sialic acid having a small molecular weight is obtained, and functions effectively as a cosmetic or hair restorer.
The solid acid refers to a solid that acts as a positive child donor or an electron acceptor, and has the property of discoloring a basic indicator or adsorbing a base like a general acid.
Silica alumina is a material in which Si and Al oxides are appropriately mixed. Most of them are amorphous, but some are crystalline. Silica alumina constitutes a multimolecular layer in a normal state.
Zeolite is a general term for aluminosilicates having fine pores in the crystal, and the Japanese name is zeolite. Widely used as molecular sieves, ion exchangers, catalysts, adsorbents, etc., zeolites having various properties are now artificially synthesized and are industrially important materials.
Acid clay is an industrial mineral resource having montmorillonite as a component together with bentonite and having strong adsorption ability and catalytic ability.

Below, the characteristic of the other component used in the cosmetics (beauty cream) of this invention is described.
-Behenyl alcohol is a reduced alcohol obtained by high-pressure hydrogen reduction of rapeseed oil and is used to increase the viscosity of the product.
・ Squalane is a saturated hydrocarbon obtained by hydrogenating squalene collected from the liver oil of sharks and sharks that live in the deep sea. It is an oily base material derived from natural products that is used in various cosmetics because it has little irritation to the skin and has a very good touch.
-Polyoxyethylene sorbitan monolaurate is a relatively safe nonionic surfactant and is used not only for emulsification purposes but also for solubilization of perfumes and the like. Macadamia nut oil is an oil obtained from the seeds of macadamia. Since it contains a large amount of oleic acid and palmitooleic acid and has a high affinity to the skin and does not give an oily feel, it feels better on the skin than other oils and fats.
-Sorbitan sesquioleate is a nonionic surfactant and is used as a lipophilic emulsifier and a dissolution aid.
-Glyceryl stearate is a kind of nonionic surfactant and is used for emulsification of creams and emulsions.
-Tocopherol is contained in vegetable oils and fats and is used as an antioxidant. -Glycerin has long been used as a moisturizer. It is used for the purpose of maintaining the consistency of the product by blending it into a cream or milky lotion, and for the purpose of adjusting the moisturizing property and feeling of use on the skin surface.
-1,3-butylene glycol is a moisturizer having little irritation and toxicity to the skin and having antibacterial properties. Widely used in creams and emulsions.
-Phenoxyethanol is a kind of antiseptic.
Acrylic acid / methacrylic acid copolymer is a base material used for adjusting the consistency of creams and gels.
Potassium hydroxide is a pH adjuster used for alkalinizing lotion.
Saitai extract is a viscous liquid containing an acidic mucopolysaccharide extracted from a mammalian umbilical cord as a raw material, and has an excellent water retention ability.
Water-soluble collagen is a kind of polypeptide obtained by extracting from mammalian connective tissue. Excellent affinity with the skin, contributing to the skin's protective effect and improved moisturizing function.

Generally, there is a cycle in hair growth, and growth and hair loss are repeated. This is called a hair cycle, and is divided into a growth period, a regression period, and a rest period. In hair growth, nourishing the hair root and moisturizing it will keep the scalp healthy, lead to a normal hair cycle, and prepare the hair growth environment.
Below, the characteristic of the other component used in the hair-growth shampoo, hair-growth conditioner, and hair-growth tonic of this invention is described.
・ Glycyrrhizic acid 2K Anti-inflammatory action ・ Chea extract Antibacterial action, active oxygen scavenging action ・ Clove extract Active oxygen scavenging action ・ Zennia oyster extract Moisturizing action, active oxygen scavenging action ・ Kihada bark extract Active oxygen scavenging action ・ Perilla extract Active oxygen scavenging action, anti-inflammation Action / Assembly extract Active oxygen scavenging action, blood flow promoting action / Lauroyl hydrolyzed silk Na hair conditioning action, moisturizing action / hydrolyzed keratin hair conditioning action, rough skin preventing action / rice fermented liquid moisturizing action / brown algae extract, red algae extract, Green algae extract Astringent action, fat decomposition action, polyquaternium-51, rough skin control, hair protection, moisturizing action, panthenol hair conditioning action, ginseng root extract cell activation action, weak acid degradation axilla extract hair growth, hair growth promoting action, sugar / amino acid Tell-1 Hair growth raw material mix, placenta extract Moisturizing, whitening, menthol refreshing, microvascular stimulating, salmon peel extract, anti-inflammatory, antioxidant, gentian root extract, antioxidant, cell activation, chamomile flower extract Anti-aging action / Tokinsenka flower extract Anti-inflammatory action, blood circulation promoting action / Comfrey leaf extract Anti-inflammatory action, conditioning action / Arnica flower extract Anti-inflammatory action, blood circulation promoting action The above sugar / amino acid ester-1 is originally The fact that amino acids are known to be important energy sources for various cells suggests that they are also important for hair matrix energy supply in hair growth, and that amino acids work on hair from the viewpoint of hair growth. Is what is expected.

Example 1 A beauty cream with excellent moisture retention and skin elasticity was obtained as follows.
Ingredient name Compounding amount (%) 1 Behenyl alcohol 3.00
2 Squalane 5.00
3 Polyoxyethylene sorbitan monolaurate 1.50
4 Macadamia nut oil 2.00
5 Sorbitan sesquioleate 0.70
6 Glyceryl stearate 0.30
7 Tocopherol 0.10
8 Purified water appropriate amount 9 Glycerin 7.00
10 1,3-butylene glycol 5.00
11 Acrylic acid / methacrylic acid copolymer 0.20
12 Phenoxyethanol appropriate amount 13 Purified water appropriate amount 14 Weak acid degradation axilla extract 1.00
15 Iwabenkei Root Extract 0.50
16 EGF (50 ppm solution) 0.40
17 Placenta extract 1.00
18 Saitai extract 0.50
19 Water-soluble collagen 0.50
20 Purified water Appropriate amount 21 Potassium hydroxide Appropriate meter 100.00
A manufacturing method and a process are as showing below.
1) Mix 1-7 and warm to 80 ° C to dissolve.
2) Mix 8-11 and warm to 80 ° C to dissolve.
3) Add 1) to 2) and uniformly emulsify.
4) Stir and cool to 60 ° C and add 12 to make uniform.
5) Stir and cool to 40 ° C and add 13-19 to make uniform.
6) Stop cooling at 35 ° C and add 20-21 to make it uniform.
7) Let stand and complete.

(Example 2) A hair-growth shampoo having an excellent hair-growth effect was obtained by blending the following main components.
Ingredient name Compounding amount (%)
1 Weak acid degradation axilla extract 5.0
2 EGF and / or FGF solution appropriate amount 3 Glycyrrhizic acid 2K 2.2
4 Cha extract 0.4
5 Clove extract 0.1
6 Mallow Extract 1.4
7 Yellowfin bark extract 0.5
8 Perilla extract 1.8
9 Assembly extract 0.5
10 Lauroyl hydrolyzed silk Na 2.5
11 Hydrolyzed keratin 0.3
12 Rice fermentation broth 0.5
13 Brown algae extract and / or red algae extract and / or green algae extract 1.6
14 Polyquaternium-51 1.8
15 Phenoxyethanol 0.8
Other shampoo components were added to make 100%.
A manufacturing method and a process are as showing below.
Suspend 10 g of axilla in 100 ml of water, add 1 ml of acetic acid, heat at 80 ° C. for 60 minutes, filter, neutralize the filtrate with sodium bicarbonate, and concentrate to dryness under reduced pressure to obtain the desired syrup. In the case of a solid acid, the target syrup can be obtained by filtering, concentrating and drying.
For weak acid: Add 5-20 to axilla 100.
For solid acid: Add 1-5 to axilla 100.
Temperature 50-100 ° C
Use water 5-10 times

(Example 3) A hair growth conditioner having an excellent hair growth effect was obtained as follows.
Ingredient name Compounding amount (%)
1 Weak acid degradation axillary extract 7.0
2 EGF and / or FGF solution appropriate amount 2 Glycyrrhizic acid 2K 2.5
3 Cha extract 1.6
4 Clove extract 0.5
5 Mallow extract 0.2
6 Yellowfin bark extract 1.9
7 Perilla extract 0.4
8 Assembly extract 6.25
9 Panthenol 0.3
10 Rice fermentation liquid 1.5
11 Polyquaternium-51 2.5
12 Phenoxyethanol 0.6
Other conditioner components were added to make 100%.
The production method and steps are the same as those in Example 2.

(Example 4) A hair-growth tonic with excellent hair-growth effect was obtained as follows.
Ingredient name Compounding amount (%)
1 Weak acid degradation axillary extract 8.5
2 EGF and / or FGF solution appropriate amount 3 Glycyrrhizic acid 2K 8.2
4 Assembly extract 5.5
5 Panax ginseng root extract 6.0
6 Sugar / amino acid ester-1 2.5
7 Menthol 0.5
8 Salamander extract 2.13
9 Rice fermentation liquid 3.4
10 Gentian root extract 0.3
11 Chamomile flower extract 2.25
12 Tokashinka Flower Extract 0.8
13 Comfrey leaf extract 2.25
14 Arnica Flower Extract 1
15 Phenoxyethanol 0.3
Other tonic components were added to make 100%.
The production method and steps are the same as those in Example 2.

The sialic acid content in the present invention is preferably 0.01 to 5 ml, more preferably 0.5 to 3 ml, particularly preferably 1 ml (the above is for 100 ml of the preparation, the same applies hereinafter).
Moreover, the EGF content in the present invention is preferably 0.00001 to 0.001 pp.
m, more preferably 0.00005 to 0.0008 ppm, particularly preferably 0
. 00008 to 0.0005 ppm (ppm represents concentration).

Respective numerical values relating to sialic acid and EGF in the present invention are shown below.
Sialic acid EGF
・ Axilla of raw materials +++ +++
・ Strong acid decomposition ++++
・ Weak acid decomposition ++ ++
・ Solid acid decomposition ++++ ++
Analysis method: A measurement kit manufactured by R & D Systems was used. Sialic acid is HPLC method, E
For GF, an ELISA kit was used and an EGF receptor antibody was used to catch the EGF receptor in the sample.
For strong acid decomposition, the mixture was heated with 10% hydrochloric acid at 100 ° C. for 60 minutes, neutralized and subjected to analysis. Weak acid decomposition uses 5% acetic acid and heats at 80 ° C. for 30 minutes.
5% was added, heated at 80 ° C. for 30 minutes, neutralized with sodium hydrogen carbonate as necessary, and used for the experiment.
In the table, ++ represents 100%, ++ represents 75% remaining, and-represents no presence.

In addition, the quantification method of sialic acid and EGF in the present invention was as follows.
Sialic acid determination method:
After acid hydrolysis, quantitative analysis of sialic acid was performed by high performance liquid chromatography (HPLC).
EGF quantification method:
Quantitative analysis of epidermal growth factor (EGF) was performed using R & D Systems' EGF ELISA kit (catalog number DEG00).

[Use test 1] (hair growth tonic)
Twenty male examinees in their 40s to 60s were asked to apply about 3 ml of the sialic acid-containing hair-restoring agent of Example 4 twice daily in the morning and evening directly to the thinned hair site, and before the application and 3 months after application, The state was compared. As a result, 17 out of 20 people improved the hair loss state, the state of the thin hair portion, and the hair thickening of the thin hair portion, and 12 of them showed a marked improvement.
As a result, it was confirmed that the sialic acid-containing hair restorer obtained in Example 4 had a remarkable or some effect on hair growth.

[Use test 2] (Beauty cream)
In the use test 2, 21 subjects composed of women in their 30s to 50s who exhibited symptoms such as dry skin, wrinkles and sagging were used as a group. In each group, the cosmetic cream obtained in Example 1 or the comparative example (sialic acid and / or EGF and / or FGF non-formulated product) was used twice a day in the morning and before going to bed for 3 months. . Furthermore, before the start of use test and 3 months after use, the pre-use value of transepidermal transpiration moisture (TEWL) on the inner side of the forearm is measured by observation of the skin condition and TEWAMETER, and the moisture of the skin is evaluated by the TEWL value. did.
From these results, the effect of the improvement of skin wrinkles, sagging, moisturizing power, and skin firmness was evaluated as a three-step evaluation, with 12 people “improving”, 6 people “slightly improving”, and 3 people “neither”. The presence or absence appeared.

The present invention can be effectively used as a cosmetic or hair restorer as described above.
It can be widely used as beauty health foods (mainly beauty health drinks), health foods for immune enhancement, and health foods for disease prevention.

Claims (10)

Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or royal jelly-derived materials from weak materials such as acetic acid, citric acid, malic acid, or lactic acid acid or silica-alumina, zeolite, decompose Ri by the solid acids such as acid clay, resulting monomolecular and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or fibroblast growth, A cosmetic or hair restorer comprising both of factors (hereinafter referred to as FGF) or a mixture of all of them. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or royal jelly-derived materials from weak materials such as acetic acid, citric acid, malic acid, or lactic acid acid or silica-alumina, zeolite, decompose Ri by the solid acids such as acid clay, resulting monomolecular and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or fibroblast growth, A mixture of both or all of these factors (hereinafter referred to as FGF), and the mixture includes behenyl alcohol, squalane, polyoxyethylene sorbitan monolaurate, macadamia nut oil, sorbitan sesquioleate, glyceryl stearate, tocopherol, glycerin, 1, 3-butylene glycol, phenoxyethanol, Acrylic acid-methacrylic acid copolymer, potassium hydroxide, Rhodiola rosea root extract, placenta extract, umbilical cord extract, cosmetic, characterized in that by blending one or more components of the water-soluble collagen. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or royal jelly-derived materials from weak materials such as acetic acid, citric acid, malic acid, or lactic acid acid or silica-alumina, zeolite, decompose Ri by the solid acids such as acid clay, resulting monomolecular and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or fibroblast growth, A mixture of all of these factors (hereinafter referred to as FGF) or a mixture of all of them, and glycyrrhizic acid 2K, tea extract, clove extract, mallow extract, yellowfin bark extract, perilla extract, assembly extract, lauroyl hydrolyzed silk Na, hydrolysis Keratin, rice fermentation broth, brown algae extract and / or red algae extract and / or Green algae extract, hair growth shampoo, characterized in that by blending one or more components of polyquaternium -51. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or royal jelly-derived materials from weak materials such as acetic acid, citric acid, malic acid, or lactic acid acid or silica-alumina, zeolite, decompose Ri by the solid acids such as acid clay, resulting monomolecular and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or fibroblast growth, A mixture of all of these factors (hereinafter referred to as FGF) or a mixture of all of them, and glycyrrhizic acid 2K, tea extract, clove extract, mallow extract, yellowfin bark extract, perilla extract, assembly extract, panthenol, rice fermentation broth, polyquaternium Hair growth condition characterized by blending one or more of -51 components Over. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or royal jelly-derived materials from weak materials such as acetic acid, citric acid, malic acid, or lactic acid acid or silica-alumina, zeolite, decompose Ri by the solid acids such as acid clay, resulting monomolecular and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or fibroblast growth, A mixture of all of these factors (hereinafter referred to as FGF) or a mixture of all of them, and glycyrrhizic acid 2K, assembly extract, ginseng extract, cephalanthin, sugar / amino acid ester-1, placenta extract, menthol, salmon peel extract, Rice fermented liquor, gentian root extract, chamomile flower extract, eucalyptus flower extract Comfrey leaf extract, hair growth tonic, characterized in that by blending one or more components of arnica flower extract. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or natural jelly-derived materials from weak acids such as acetic acid, citric acid, malic acid, or lactic acid Or by decomposition with a solid acid such as silica alumina, zeolite, acidic clay, etc., and unimolecular and low molecular sialic acid and epidermal growth factor (hereinafter referred to as EGF) and / or fibroblast growth factor (hereinafter referred to as FGF). both or wherein Rukoto give all these mixtures, the production method of the cosmetic or hair-growing agent referred). Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or natural jelly-derived materials from weak acids such as acetic acid, citric acid, malic acid, or lactic acid Or mono- and low-molecular-weight sialic acid and epithelial cell growth factor (hereinafter referred to as EGF) and / or fibroblast growth factor (hereinafter referred to as the following) obtained by decomposition with a solid acid such as silica alumina, zeolite or acidic clay. , FGF), or a mixture of all of these , behenyl alcohol, squalane, polyoxyethylene sorbitan monolaurate, macadamia nut oil, sorbitan sesquioleate, glyceryl stearate, tocopherol, glycerin, 1,3-butylene glycol, phenoxyethanol , Acrylic acid, meta Acrylic acid copolymer, potassium hydroxide, Rhodiola rosea root extract, placenta extract, umbilical cord extract, cosmetic method of manufacturing which is characterized in that by blending one or more components of the water-soluble collagen. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or natural jelly-derived materials from weak acids such as acetic acid, citric acid, malic acid, or lactic acid Or mono- and low-molecular-weight sialic acid and epithelial cell growth factor (hereinafter referred to as EGF) and / or fibroblast growth factor (hereinafter referred to as the following) obtained by decomposition with a solid acid such as silica alumina, zeolite or acidic clay. , to both or all mixtures of these referred to as FGF), glycyrrhizic acid 2K, green tea extract, clove extract, Zegna Oy extract, Phellodendron bark extract, mint extract, assembly extract, lauroyl hydrolyzed silk Na, hydrolyzed keratin, rice fermentation liquid, Brown algae extract and / or red algae extract and / or green algae extract Method for producing a hair growth shampoo, characterized in that by blending one or more components of the Quaternium -51. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or natural jelly-derived materials from weak acids such as acetic acid, citric acid, malic acid, or lactic acid Or mono- and low-molecular-weight sialic acid and epithelial cell growth factor (hereinafter referred to as EGF) and / or fibroblast growth factor (hereinafter referred to as the following) obtained by decomposition with a solid acid such as silica alumina, zeolite or acidic clay. , to both or all mixtures of these referred to as FGF), glycyrrhizic acid 2K, green tea extract, clove extract, Zegna Oy extract, Phellodendron bark extract, mint extract, assembly extract, panthenol, rice fermented liquor, one of the polyquaternium -51 A method for producing a hair-growth conditioner comprising the above ingredients. Swallow's nest extract (hereinafter referred to as axillary extract) and / or animal (including cattle) saliva and / or natural jelly-derived materials from weak acids such as acetic acid, citric acid, malic acid, or lactic acid Or mono- and low-molecular-weight sialic acid and epithelial cell growth factor (hereinafter referred to as EGF) and / or fibroblast growth factor (hereinafter referred to as the following) obtained by decomposition with a solid acid such as silica alumina, zeolite or acidic clay. , to both or all mixtures of these referred to as FGF), glycyrrhizic acid 2K, assembly extract, ginseng extract, cepharanthine, sugar / amino acid ester over 1, placenta extract, menthol, pepper peel extract, fermented rice liquor, gentian root extract , Chamomile flower extract, flowering flower extract, comfrey leaves Scan method of hair restoration tonic, characterized in that by blending one or more components of arnica flower extract.