CN113730306A - Anti-aging composition, anti-aging vesicle and preparation method and application of anti-aging vesicle - Google Patents
Anti-aging composition, anti-aging vesicle and preparation method and application of anti-aging vesicle Download PDFInfo
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Abstract
The invention discloses an anti-aging composition, an anti-aging vesicle and a preparation method and application thereof, wherein the anti-aging composition comprises giant salamander polypeptide, dendrobium officinale polysaccharide, tea polyphenol, hyaluronic acid, ophiopogon root polysaccharide, lycium barbarum polysaccharide and grape seed oil; the anti-aging composition is encapsulated by lipid vesicles; the anti-aging vesicle comprises, by weight, 0.05-25 parts of an anti-aging composition, 20-50 parts of a nano lipid vesicle and 5-66 parts of water; and the application of the anti-aging composition and the anti-aging in the preparation of cosmetics. The anti-aging composition has an obvious effect of delaying skin aging, and is mild in effect and free of irritation to skin. On the basis, the lipid vesicles and the anti-aging composition are used in a synergistic manner by being encapsulated by the lipid vesicles, so that the skin-care and anti-aging effects with better effects are realized.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging composition, an anti-aging vesicle and a preparation method and application thereof.
Background
The popularity of the cosmetic industry continues to increase due to factors such as increased permeability, increased consumption frequency, and increased core cosmetic population. In 2019, the domestic cosmetic society increases the speed by 12.1% in 1-10 months, is higher than the total sum of the social consumer goods in the third year continuously, and is positioned on the second of the zero 14 subsidiary products of society. Along with the rapid expansion of the cosmetic consumer market, the requirements of consumers on the product quality and the product efficacy are higher and higher.
The skin is the most intuitive characterization of human age, and the youthful skin state with good elasticity, luster and enough moisture is one of the main purposes of skin care of consumers, and correspondingly, the skin care product for delaying the fire explosion of the aging class in the market. However, the efficacy of many skin care products for delaying senility is not obvious at present, and some products are only used for concept publicity, which is contradictory to the pursuit of the efficacy by consumers.
Therefore, the improvement of the skin care effect of the anti-aging cosmetics is an urgent problem to be solved in the process of realizing the efficacy of the anti-aging cosmetics.
Disclosure of Invention
Aiming at the problems in the background art, the invention aims to provide an anti-aging composition, an anti-aging vesicle and a preparation method and application thereof, which realize the purpose of delaying skin aging through the matching of all components and are mild and non-irritant.
In order to achieve the purpose, the technical scheme of the invention is as follows:
first, the present invention provides an anti-aging composition, comprising: giant salamander polypeptide, dendrobium officinale polysaccharide, tea polyphenol, hyaluronic acid, ophiopogon root polysaccharide, wolfberry polysaccharide and grape seed oil.
As an optimal scheme, the mass ratio of the giant salamander polypeptide to the dendrobium officinale polysaccharide to the tea polyphenol to the hyaluronic acid to the ophiopogon polysaccharide to the lycium barbarum polysaccharide to the grape seed oil is 0.5-8: 1-5: 0.2-0.8: 0.8-4: 0.8-2: 0.8-2: 1.2 to 2.
Preferably, the mass ratio of the giant salamander polypeptide to the dendrobium officinale polysaccharide to the tea polyphenol to the hyaluronic acid to the ophiopogon japonicus polysaccharide to the lycium barbarum polysaccharide to the grape seed oil is 0.5: 5: 0.8: 0.8: 2: 2: 2 or 4: 3: 0.4: 1: 1.4: 1.4: 1.8 or 6: 1: 0.2: 2: 1.2: 1.2: 1.6 or 8: 1: 0.5: 3: 1: 1: 1.4 or 8: 1: 0.2: 4: 0.8: 0.8: 1.2.
in the invention, the preparation method of the dendrobium officinale polysaccharide, the ophiopogon root polysaccharide and the lycium barbarum polysaccharide comprises the following steps:
s1, adding the medicinal materials into water of which the amount is 12 times that of the medicinal materials, decocting for 1.0-2.0 hours, and filtering to obtain filtrate and filter residues;
s2, concentrating the water extract under reduced pressure at 80 ℃, adding 70% ethanol with 4 times of volume while stirring, and standing for 12 hours;
s3, separating the precipitate, washing with 70% ethanol, and vacuum freeze-drying to obtain Dendrobium officinale polysaccharide, ophiopogon root polysaccharide and lycium barbarum polysaccharide.
Preferably, the filter residue filtered in the S1 is added with 10 times of water and decocted for 0.5-1.0 h, filtered, and the two filtrates are combined and then subjected to S2.
In the invention, the preparation method of the giant salamander polypeptide comprises the following steps:
s1, mincing the clean giant salamander meat by a meat mincer to obtain giant salamander minced meat;
s2, adding deionized water into the giant salamander minced meat, and adding compound protease; the enzymolysis conditions are as follows: the ratio of material to liquid is 1: 25, adding 2000U/g of compound protease, keeping the temperature at 60 ℃, and carrying out enzymolysis for 7 hours;
s3, centrifuging the enzymatic hydrolysate to obtain a supernatant, carrying out reduced pressure concentration on the supernatant at 50-55 ℃, and then carrying out spray drying to obtain the giant salamander polypeptide powder.
Secondly, in order to obtain better skin care effect of the anti-aging composition provided by the invention, the invention provides a using method of the anti-aging composition, wherein the anti-aging composition is used by being encapsulated in lipid vesicles.
Preferably, the lipid vesicle is a nano lipid vesicle.
Thirdly, the invention provides an anti-aging vesicle encapsulating the anti-aging composition, wherein the anti-aging vesicle comprises, by weight, 0.05-25 parts of the anti-aging composition, 20-50 parts of the nano lipid vesicle and 5-66 parts of water.
Preferably, the nano lipid vesicle comprises the following components in parts by weight: 6.5-18.0 parts of nonionic surfactant, 10-25 parts of water-soluble alcohol, 2.0-8.0 parts of composite phospholipid and 1.0-4.5 parts of cholesterol.
Further preferably, the nonionic surfactant is one or more of polysorbate-20, polysorbate-80, polyglycerol-10 oleate, polyglycerol-3 methyl glucose distearate and C12-C14 alkanol polyether-12.
Further preferably, the compound phospholipid is one or a combination of more of soybean lecithin PC-50, egg yolk lecithin and hydrogenated lecithin.
Fourthly, the invention provides a method for preparing the anti-aging vesicle, which is characterized by comprising the following steps:
s1, mixing the composite phospholipid, cholesterol, the water-insoluble component in the anti-aging composition and the surfactant to obtain an oil phase;
s2, mixing the water-soluble component, the water-soluble alcohol and the water in the anti-aging composition to obtain a water phase;
s3, mixing the oil phase in the S1 and the water phase in the S2, and then sequentially carrying out rapid shearing to obtain a micro-emulsion;
and S4, homogenizing the microemulsion in the S3 under high pressure to obtain the anti-aging vesicle.
Preferably, in S3, the rotation speed of the shearing is 6000-13000 rpm, the shearing time is 2-10 min,
preferably, in S4, the homogenizing pressure is 600-900 bar, and the homogenizing times are 2-3 times.
Fifth, the present invention provides the use of the anti-aging composition as described in the first aspect for the preparation of cosmetics.
Sixth, the present invention provides the use of the anti-aging vesicle according to the third aspect in the preparation of cosmetics.
Compared with the prior art, the invention has the beneficial effects that:
the anti-aging composition has an obvious effect of delaying skin aging, and is mild in effect and free of irritation to skin. On the basis, the lipid vesicles and the anti-aging composition are used in a synergistic manner by being encapsulated by the lipid vesicles, so that the skin-care and anti-aging effects with better effects are realized.
Drawings
FIG. 1 is a graph showing the survival rate (%)
FIG. 2 is a graph showing the survival rate (%)
FIG. 3 is a graph showing the survival rate (%)
FIG. 4 is a graph showing the survival rate (%)
Detailed Description
The technical solutions of the present invention, including the preferred technical solutions, are described in further detail below by way of some alternative embodiments of the present invention. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.
The invention provides an anti-aging composition, which is characterized by comprising the following components: giant salamander polypeptide, dendrobium officinale polysaccharide, tea polyphenol, hyaluronic acid, ophiopogon root polysaccharide, wolfberry polysaccharide and grape seed oil.
The components of the anti-aging composition can be obtained by extracting according to a conventional method, but the following obtaining methods are provided in the specific implementation process of the invention:
1) the preparation method of the dendrobium officinale polysaccharide, the ophiopogon root polysaccharide and the lycium barbarum polysaccharide comprises the following steps: adding the medicinal materials into 12 times of water, decocting for 1.0-2.0 h, and filtering to obtain filtrate and filter residue; adding 10 times of water into filter residue, decocting for 0.5-1.0 h, filtering, combining the two filtrates, concentrating the water extract under reduced pressure at 80 ℃, adding 4 times of 70% ethanol in volume while stirring, and standing for 12 h; separating the precipitate, washing with 70% ethanol, and vacuum freeze drying to obtain Dendrobium officinale polysaccharide, radix Ophiopogonis polysaccharide, and fructus Lycii polysaccharide.
2) The preparation method of the giant salamander polypeptide comprises the following steps: mincing the clean giant salamander meat by a meat mincer to obtain giant salamander minced meat, adding deionized water into the giant salamander minced meat, wherein the enzymolysis conditions are as follows: the ratio of material to liquid is 1: 25, adding 2000U/g of compound protease, keeping the temperature at 60 ℃, and carrying out enzymolysis for 7 hours. And centrifuging the enzymolysis solution to obtain a supernatant, carrying out reduced pressure concentration on the supernatant at 50-55 ℃, and then carrying out spray drying to obtain the giant salamander polypeptide powder.
3) The sources of the tea polyphenol, the hyaluronic acid and the grape seed oil are not particularly limited, and the conventional commercially available cosmetic-grade products are adopted.
Example 1: the anti-aging composition
0.5 part of giant salamander polypeptide, 5 parts of dendrobium officinale polysaccharide, 0.8 part of tea polyphenol, 0.8 part of hyaluronic acid, 0.8 part of ophiopogon polysaccharide, 2 parts of wolfberry polysaccharide and 2 parts of grape seed oil;
example 2: the anti-aging composition
Giant salamander polypeptide 4, dendrobium officinale polysaccharide 3, tea polyphenol 0.4, hyaluronic acid 1, ophiopogon polysaccharide 1.4, wolfberry polysaccharide 1.4 and grape seed oil 1.8;
example 3: the anti-aging composition
Giant salamander polypeptide 6, dendrobium officinale polysaccharide 1, tea polyphenol 0.2, hyaluronic acid 2, ophiopogon japonicus polysaccharide 1.2, wolfberry polysaccharide 1.2 and grape seed oil 1.6;
example 4: the anti-aging composition
Giant salamander polypeptide 8, dendrobium officinale polysaccharide 1, tea polyphenol 0.5, hyaluronic acid 3, ophiopogon root polysaccharide 1, wolfberry polysaccharide 1 and grape seed oil 1.4;
example 5: the anti-aging composition
Giant salamander polypeptide 8, dendrobium officinale polysaccharide 1, tea polyphenol 0.2, hyaluronic acid 4, ophiopogon japonicus polysaccharide 1.8, wolfberry polysaccharide 0.8 and grape seed oil 1.2;
in the specific implementation process of the invention, according to parts by weight, the anti-aging composition comprises 5-80 parts of giant salamander polypeptide, 10-50 parts of dendrobium officinale polysaccharide, 2-8 parts of tea polyphenol, 8-40 parts of hyaluronic acid, 8-20 parts of ophiopogon polysaccharide, 8-20 parts of lycium barbarum polysaccharide and 12-20 parts of grape seed oil; the parts by weight may each represent any number in any unit of weight, for example a part by weight may represent 1g, 1kg etc.
Example 6: the invention provides anti-aging vesicles and a preparation method thereof
The anti-ageing vesicles in this example comprise, by weight: 14 parts of the anti-ageing composition of example 2, 32 parts of the nano-lipid vesicles, 54 parts of water;
the preparation method comprises
Mixing 5% of 1, 3-butanediol, 7% of propylene glycol, 4.3% of giant salamander polypeptide, 3.2% of dendrobium officinale polysaccharide, 0.4% of tea polyphenol, 1.1% of hyaluronic acid, 1.5% of ophiopogon polysaccharide and 1.5% of lycium barbarum polysaccharide with the balance of water according to mass percentage, and stirring at 55 ℃ in a water bath to obtain a water phase for later use;
melting 5% of soybean lecithin PC-50, 3% of hydrogenated lecithin, 2% of cholesterol, 10% of polyglycerol-3 methyl glucose distearate and 2% of grape seed oil in a water bath at 55 ℃ to obtain an oil phase for later use;
after the water phase and the oil phase are mixed, quickly shearing, wherein the conditions are as follows: shearing at 10000rpm for 2min to obtain microemulsion for use;
carrying out high-pressure homogenization on the microemulsion under the conditions: homogenizing at 800bar for 2 times to obtain anti-aging vesicle.
The particle size of the anti-aging vesicle is detected, and the particle size of the anti-aging vesicle is 90.2nm, and the PDI (polydispersity index) is 0.236.
Example 7: the invention provides anti-aging vesicles and a preparation method thereof
The anti-ageing vesicles in this example comprise, by weight: 10 parts of the anti-ageing composition of example 2, 36 parts of the nano-lipid vesicles, 54 parts of water;
the preparation method comprises
Mixing 7% of 1, 3-butanediol, 6% of propylene glycol, 1% of polyethylene glycol, 3.1% of giant salamander polypeptide, 2.3% of dendrobium officinale polysaccharide, 0.3% of tea polyphenol, 0.8% of hyaluronic acid, 1.1% of ophiopogon root polysaccharide and 1.1% of lycium barbarum polysaccharide with the balance of water in percentage by mass, and stirring in a water bath at 55 ℃ to obtain a water phase for later use;
melting 2% soybean lecithin PC-50, 6% hydrogenated lecithin, 2% cholesterol, 12% C12-C14 alkanol polyether-12 and 1.4% grape seed oil in water bath at 55 deg.C to obtain oil phase;
after the water phase and the oil phase are mixed, quickly shearing, wherein the conditions are as follows: shearing at 9000rpm for 2min to obtain microemulsion for use;
carrying out high-pressure homogenization on the microemulsion under the conditions: homogenizing under 900bar for 2 times to obtain anti-aging vesicle.
And detecting the particle size of the anti-aging vesicle to obtain the anti-aging vesicle with the particle size of 82.6nm and the PDI of 0.283.
Comparative example 1:
the giant salamander polypeptide is removed in composition in example 2, and specifically comprises: 0.4 parts of tea polyphenol, 1 part of hyaluronic acid, 1.4 parts of ophiopogonpolysaccharide, 1.4 parts of lycium barbarum polysaccharide and 1.8 parts of grape seed oil;
comparative example 2:
the method for replacing the giant salamander polypeptide with VC in the composition of the example 2 specifically comprises the following steps: VC4, dendrobium officinale polysaccharide 3, tea polyphenol 0.4, hyaluronic acid 1, ophiopogon polysaccharide 1.4, wolfberry polysaccharide 1.4 and grape seed oil 1.8;
comparative example 3:
this comparative example is the same as example 6, except that the composition of the antiaging composition used in comparative example 1 was used.
Comparative example 4:
this comparative example is the same as example 6 except that the composition of the antiaging composition used in comparative example 2 was used.
The following evaluations were made with respect to the above examples and comparative examples.
Example 8 evaluation of stability of anti-aging vesicles
The anti-aging vesicles prepared in examples 6 and 7 were placed in a closed container at room temperature for 30 days, 60 days, and 90 days, 200ul of each sample was diluted with ultrapure water 100 times, and then the anti-aging vesicles were dispersed uniformly by ultrasound, and the particle size, PDI, and Zeta potential of the anti-aging vesicles were measured by a nano-particle sizer, and the specific measurement results are shown in table 1.
TABLE 1 anti-ageing vesicle stability test results
As can be seen from table 1, the anti-aging vesicle samples prepared in examples 6 and 7 do not have aggregation, delamination and discoloration after being placed for 90 days, and the particle size, PDI and Zeta potential data show that the particle size of the samples is small (between 50 nm and 100 nm), the PDI shows that the anti-aging vesicle has good dispersibility, the Zeta potential shows that the anti-aging vesicle samples still maintain high potential after being placed for 90 days, and the anti-aging vesicle samples have good stability.
Example 9 evaluation of safety-human fibroblasts and human keratinocytes
Cell plating: human normal skin fibroblasts (HSF) and human immortalized keratinocytes (Hacat) were plated in 96-well plates at 6000 and 12000 cells per well, respectively, and cultured for 24 hours for use.
Sample preparation: in a clean bench, samples of example 2, example 6 and example 7 are dissolved in a serum-containing culture medium, and the concentration of each sample in examples 6 and 7 is 10mg/mL, 5mg/mL, 3mg/mL, 1mg/mL, 0.5mg/mL, 0.3mg/mL and 0.1mg/mL in turn, calculated by the content mass concentration of the anti-aging composition.
Sample adding: extracting culture solution from a 96-well plate of HSF and Hacat cells cultured for 24 hours, and sequentially adding culture media (sample groups) with different concentrations; meanwhile, setting a control group, namely a cell and normal serum-containing culture medium group; background subtraction group, i.e. the group of medium without laying cells only, was used to subtract the interference of the culture plate. Each set was provided with 5 parallel wells, to each of which 100. mu.L of sample was added.
Detecting the cell proliferation rate: after the sample was added, the culture was continued in the incubator for 24 hours, and the sample was taken out and the percentage of cell proliferation was measured by the SRB method.
The detection results are shown in fig. 1 and fig. 2, which show that the anti-aging composition solutions of examples 2, 6 and 7 with different concentrations do not affect the survival of HSF and Hacat cells, have no cytotoxicity and certain proliferation promoting capacity, and indicate that the anti-aging composition and the anti-aging vesicles thereof do not generate irritation on epidermis.
Example 10 Oxidation resistance test
Cell plating: human normal skin fibroblasts (HSF) and human immortalized keratinocytes (Hacat) were plated in 96-well plates at 6000 and 12000 cells per well, respectively, and cultured for 24 hours for use.
Hydrogen peroxide solution and sample configuration: in a clean bench, hydrogen peroxide was dissolved in the culture solution to prepare a solution having a concentration of 600. mu. mol/L, and the solution was sterilized by filtration using a 0.22 μm filter head. The samples of examples 1-7 were dissolved in the culture solution containing H2O2, respectively, to prepare 1mg/mL (in terms of anti-aging composition) of sample for use.
Sample adding: the culture medium in a 96-well plate of HSF and Hacat cells cultured for 24 hours was aspirated, and the culture medium containing 600. mu. mol/L H2O2 (group H2O 2) and the culture medium containing 600. mu. mol/L H2O2 and 1mg/mL of the anti-aging composition (examples 1-7) were added in this order; setting a control group at the same time, namely a cell and normal culture solution group; and background subtraction group, namely a group which is only added with culture solution without laying cells and is used for subtracting the interference of the culture plate. Each set was provided with 5 parallel wells, to each of which 100. mu.L of sample was added.
Detecting the cell proliferation rate: after the sample was added, the culture was continued in the incubator for 24 hours, and the sample was taken out and the percentage of cell proliferation was measured by the SRB method.
The results are shown in fig. 3 and 4, the survival rates of HSF cells and Hacat cells added into the culture solution of human normal skin fibroblasts and human immortalized keratinocytes are remarkably reduced, and the modeling result of a hydrogen peroxide injury model is proved. The experimental results show that the hydrogen peroxide and one of the examples 1-7 and the comparative examples 1-2 can repair the oxidative damage of the hydrogen peroxide to the cell irradiation, particularly HSF cells, but the effect of the comparative examples 1-2 is obviously lower than that of the examples 1-7, particularly the examples 6-7, the same trend is also shown in the survival rate of Hacat cells in figure 4, and the anti-aging composition provided by the invention is sequentially proved to have obvious anti-oxidative damage efficacy, and the anti-aging vesicle encapsulation can play a synergistic role, has anti-aging skin care activity and can be used as an active raw material for developing anti-aging cosmetics.
Example 11 human efficacy
Blank sample: and (3) filling the cut mask non-woven fabrics and 30ml of the blank mask essence formula (without adding functional substances) in the table 2 into an aluminum foil bag, and sealing to obtain a blank sample to be tested.
Sample group: 1% of the anti-aging compositions of examples 1 to 5, 6 to 7 of anti-aging vesicles (1% of the anti-aging compositions) and 1% to 2% of comparative examples 1 to 2 (1% of the anti-aging compositions) are respectively added into the blank facial mask essence formula in the table 2 to obtain facial mask essence formulas of examples 1 to 7 and comparative examples 1 to 2 for later use; filling the cut mask non-woven fabrics and 30ml of mask essence into an aluminum foil bag, and sealing the aluminum foil bag to obtain mask samples to be tested in examples 1-7 and comparative examples 1-2;
TABLE 2 blank facial mask essence formula
Components | Mass fraction (parts) | Components | Mass fraction (parts) |
Glycerol | 10.0 | Butanediol | 5.0 |
EDTA-2Na | 0.05 | Tween-80 | 0.5 |
Functional substance | 5.00 | Phenoxyethanol | 0.5 |
Essence | 0.05 | Water (W) | Balance of |
50 volunteers were randomly recruited as subjects, and 10 groups were assigned to one of the subjects (blank group, examples 1 to 7, comparative examples 1 to 2). Cheeks were used as test sites on the face of the subject. After cleansing, skin texture and elasticity test was performed on the skin test area using the skin analysis tester VISIA and the skin elasticity tester MPA 580, and the experimental data was recorded, and then the mask was applied to the face, and the experimental data of the test area of the face of the subject was tested again at the corresponding time.
The skin texture and skin elasticity of the test area of the face of the subject were measured by the same person using VISIA before, after 4 weeks and 8 weeks of continuous use of the product (continuous use of the product means use every 4 days). The results are shown in tables 3 and 4.
TABLE 3 skin texture Change
TABLE 4 changes in skin elasticity
As can be seen from tables 3 and 4: compared with comparative examples 1-2, the anti-aging effects of examples 1-7 are remarkable, the average skin surface wrinkles are remarkably reduced, and the skin elasticity is remarkably increased, so that the anti-aging composition and the lipid vesicles thereof have remarkable anti-aging capability for the skin, and particularly the anti-aging composition encapsulated by the lipid vesicles has better effects.
In conclusion, the anti-aging composition and the lipid vesicles thereof have a remarkable anti-aging effect, and the effect is better after the continuous use for the eighth week, so that the anti-aging composition and the lipid vesicles thereof have a long-acting anti-aging effect.
It should be emphasized that the above detailed description is specific to possible embodiments of the invention, which are not intended to limit the scope of the invention, but rather are intended to include equivalents and modifications, all without departing from the technical spirit of the invention.
It will be understood by those skilled in the art that the foregoing is only a preferred embodiment of the present invention, and is not intended to limit the invention, and any modification, combination, replacement, or improvement made within the spirit and principle of the present invention is included in the scope of the present invention.
Claims (10)
1. An anti-aging composition, comprising: giant salamander polypeptide, dendrobium officinale polysaccharide, tea polyphenol, hyaluronic acid, ophiopogon root polysaccharide, wolfberry polysaccharide and grape seed oil, wherein the mass ratio of the giant salamander polypeptide to the dendrobium officinale polysaccharide to the grape seed oil is 0.5-8: 1-5: 0.2-0.8: 0.8-4: 0.8-2: 0.8-2: 1.2 to 2.
2. The anti-aging composition according to claim 1, characterized in that: the preparation method of the dendrobium officinale polysaccharide, the ophiopogon root polysaccharide and the lycium barbarum polysaccharide comprises the following steps:
s1, adding the medicinal materials into water of which the amount is 12 times that of the medicinal materials, decocting for 1.0-2.0 hours, and filtering to obtain filtrate and filter residues; adding 10 times of water into the filter residue, decocting for 0.5-1.0 h, filtering, and combining the two filtrates;
s2, concentrating the water extract under reduced pressure at 80 ℃, adding 70% ethanol with 4 times of volume while stirring, and standing for 12 hours;
s3, separating the precipitate, washing with 70% ethanol, and vacuum freeze-drying to obtain Dendrobium officinale polysaccharide, ophiopogon root polysaccharide and lycium barbarum polysaccharide.
3. The anti-aging composition according to claim 1, characterized in that: the preparation method of the giant salamander polypeptide comprises the following steps:
s1, mincing the clean giant salamander meat by a meat mincer to obtain giant salamander minced meat;
s2, adding deionized water into the giant salamander minced meat, and adding compound protease; the enzymolysis conditions are as follows: the ratio of material to liquid is 1: 25, adding 2000U/g of compound protease, keeping the temperature at 60 ℃, and carrying out enzymolysis for 7 hours;
s3, centrifuging the enzymatic hydrolysate to obtain a supernatant, carrying out reduced pressure concentration on the supernatant at 50-55 ℃, and then carrying out spray drying to obtain the giant salamander polypeptide powder.
4. A method for using an anti-aging composition, wherein the anti-aging composition is used by being encapsulated in lipid vesicles according to any one of claims 1 to 4; the lipid vesicle is a nano lipid vesicle.
5. An anti-ageing vesicle encapsulating an anti-ageing composition as claimed in claims 1 to 3, comprising: 0.05-25 parts of an anti-aging composition, 20-50 parts of nano lipid vesicles and 5-66 parts of water.
6. The anti-senescence vesicle according to claim 5, wherein: the nano lipid vesicle comprises the following components in parts by weight: 6.5-18.0 parts of nonionic surfactant, 10-25 parts of water-soluble alcohol, 2.0-8.0 parts of composite phospholipid and 1.0-4.5 parts of cholesterol.
7. The anti-senescence vesicle according to claim 6, wherein: the nonionic surfactant is one or more of polysorbate-20, polysorbate-80, polyglycerol-10 oleate, polyglycerol-3 methyl glucose distearate, and C12-C14 alkanol polyether-12.
8. The anti-senescence vesicle according to claim 6, wherein: the compound phospholipid is one or more of soybean lecithin PC-50, yolk lecithin and hydrogenated lecithin.
9. A process for the preparation of anti-ageing vesicles according to claims 5 to 8, comprising the following steps:
s1, mixing the composite phospholipid, cholesterol, the water-insoluble component in the anti-aging composition and the surfactant to obtain an oil phase;
s2, mixing the water-soluble component, the water-soluble alcohol and the water in the anti-aging composition to obtain a water phase;
s3, mixing the oil phase in the S1 and the water phase in the S2, and then sequentially carrying out rapid shearing to obtain a micro-emulsion; the shearing rotating speed is 6000-13000 rpm, and the shearing time is 2-10 min;
and S4, homogenizing the microemulsion in the S3 at high pressure, wherein the homogenizing pressure is 600-900 bar, and the homogenizing times are 2-3 times, so that the anti-aging vesicle is obtained.
10. Use of an anti-ageing composition according to any one of claims 1 to 3 or an anti-ageing vesicle according to any one of claims 5 to 8 in the preparation of a cosmetic product.
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