CN112237284A - Application of wild cherry juice in inhibiting skin aging - Google Patents
Application of wild cherry juice in inhibiting skin aging Download PDFInfo
- Publication number
- CN112237284A CN112237284A CN202010695659.9A CN202010695659A CN112237284A CN 112237284 A CN112237284 A CN 112237284A CN 202010695659 A CN202010695659 A CN 202010695659A CN 112237284 A CN112237284 A CN 112237284A
- Authority
- CN
- China
- Prior art keywords
- skin
- juice
- composition
- wild cherry
- agents
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000011389 fruit/vegetable juice Nutrition 0.000 title claims abstract description 138
- 244000007021 Prunus avium Species 0.000 title claims abstract description 74
- 235000010401 Prunus avium Nutrition 0.000 title claims abstract description 74
- 235000014441 Prunus serotina Nutrition 0.000 title claims abstract description 74
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 14
- 230000009759 skin aging Effects 0.000 title abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 38
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 claims abstract description 31
- 210000001626 skin fibroblast Anatomy 0.000 claims abstract description 10
- 230000035755 proliferation Effects 0.000 claims abstract description 7
- 230000001737 promoting effect Effects 0.000 claims abstract description 6
- 235000000661 Rosa laevigata Nutrition 0.000 claims description 40
- 241001278833 Rosa laevigata Species 0.000 claims description 40
- 239000011148 porous material Substances 0.000 claims description 35
- 230000037303 wrinkles Effects 0.000 claims description 18
- 239000003642 reactive oxygen metabolite Substances 0.000 claims description 12
- 230000037394 skin elasticity Effects 0.000 claims description 10
- 239000008194 pharmaceutical composition Substances 0.000 claims description 9
- 239000002537 cosmetic Substances 0.000 claims description 4
- 239000002417 nutraceutical Substances 0.000 claims description 4
- 235000021436 nutraceutical agent Nutrition 0.000 claims description 4
- 230000000699 topical effect Effects 0.000 claims description 4
- 230000037373 wrinkle formation Effects 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 11
- 239000001301 oxygen Substances 0.000 abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 abstract description 11
- 210000003491 skin Anatomy 0.000 description 132
- 210000004027 cell Anatomy 0.000 description 60
- 239000000243 solution Substances 0.000 description 45
- 239000003795 chemical substances by application Substances 0.000 description 25
- 238000002474 experimental method Methods 0.000 description 23
- 230000001815 facial effect Effects 0.000 description 23
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 22
- 239000002953 phosphate buffered saline Substances 0.000 description 22
- 235000013361 beverage Nutrition 0.000 description 19
- 108010035532 Collagen Proteins 0.000 description 18
- 102000008186 Collagen Human genes 0.000 description 18
- 229920001436 collagen Polymers 0.000 description 18
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 16
- 239000002609 medium Substances 0.000 description 16
- 230000035622 drinking Effects 0.000 description 15
- 239000000047 product Substances 0.000 description 15
- 238000000034 method Methods 0.000 description 13
- 210000002950 fibroblast Anatomy 0.000 description 12
- 239000000523 sample Substances 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000000654 additive Substances 0.000 description 10
- 239000000902 placebo Substances 0.000 description 10
- 229940068196 placebo Drugs 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000000996 additive effect Effects 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000003349 gelling agent Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 150000003254 radicals Chemical class 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 230000003712 anti-aging effect Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 239000002738 chelating agent Substances 0.000 description 5
- 239000003995 emulsifying agent Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000002932 luster Substances 0.000 description 5
- 239000002674 ointment Substances 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 239000001993 wax Substances 0.000 description 5
- 240000005662 Aronia melanocarpa Species 0.000 description 4
- 235000007425 Aronia melanocarpa Nutrition 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 4
- 239000006096 absorbing agent Substances 0.000 description 4
- 239000002671 adjuvant Substances 0.000 description 4
- 230000032683 aging Effects 0.000 description 4
- 239000006071 cream Substances 0.000 description 4
- 239000003937 drug carrier Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000003755 preservative agent Substances 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 241000435509 Prunus wilsonii Species 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 239000000834 fixative Substances 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 230000001678 irradiating effect Effects 0.000 description 3
- 235000001032 juan mao chou li Nutrition 0.000 description 3
- 239000000865 liniment Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 201000001441 melanoma Diseases 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 230000036548 skin texture Effects 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000002562 thickening agent Substances 0.000 description 3
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- VFNKZQNIXUFLBC-UHFFFAOYSA-N 2',7'-dichlorofluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(Cl)=C(O)C=C1OC1=C2C=C(Cl)C(O)=C1 VFNKZQNIXUFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000012099 Alexa Fluor family Substances 0.000 description 2
- 241001444063 Aronia Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000195940 Bryophyta Species 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- 201000004624 Dermatitis Diseases 0.000 description 2
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 108010014258 Elastin Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 2
- 235000015533 Prunus davidiana Nutrition 0.000 description 2
- 240000002381 Prunus davidiana Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000000692 Student's t-test Methods 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 239000003070 absorption delaying agent Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000058 anti acne agent Substances 0.000 description 2
- 229940124340 antiacne agent Drugs 0.000 description 2
- 239000012984 antibiotic solution Substances 0.000 description 2
- 239000003908 antipruritic agent Substances 0.000 description 2
- 235000015173 baked goods and baking mixes Nutrition 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 239000007844 bleaching agent Substances 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 2
- 235000005487 catechin Nutrition 0.000 description 2
- 238000002737 cell proliferation kit Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229950001002 cianidanol Drugs 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 235000015872 dietary supplement Nutrition 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 239000012154 double-distilled water Substances 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 235000021107 fermented food Nutrition 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 2
- 239000002085 irritant Substances 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 239000000314 lubricant Substances 0.000 description 2
- 230000008099 melanin synthesis Effects 0.000 description 2
- 210000002752 melanocyte Anatomy 0.000 description 2
- 229940016286 microcrystalline cellulose Drugs 0.000 description 2
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 2
- 239000008108 microcrystalline cellulose Substances 0.000 description 2
- 239000007758 minimum essential medium Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000011929 mousse Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000003002 pH adjusting agent Substances 0.000 description 2
- -1 patches (patches) Substances 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 235000019271 petrolatum Nutrition 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000003380 propellant Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- XDFNWJDGWJVGGN-UHFFFAOYSA-N 2-(2,7-dichloro-3,6-dihydroxy-9h-xanthen-9-yl)benzoic acid Chemical compound OC(=O)C1=CC=CC=C1C1C2=CC(Cl)=C(O)C=C2OC2=CC(O)=C(Cl)C=C21 XDFNWJDGWJVGGN-UHFFFAOYSA-N 0.000 description 1
- KQNVYRKIBZXKGG-UHFFFAOYSA-N 2-methylpyridine azide Chemical compound CC1=NC=CC=C1.[N-]=[N+]=[N-] KQNVYRKIBZXKGG-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 239000004150 EU approved colour Substances 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 108010050808 Procollagen Proteins 0.000 description 1
- 241001506873 Prunus cerasifera Species 0.000 description 1
- 235000009836 Prunus pissardii Nutrition 0.000 description 1
- 241000392970 Prunus serrulata Species 0.000 description 1
- 235000014001 Prunus serrulata Nutrition 0.000 description 1
- 206010037867 Rash macular Diseases 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 235000013932 Rosa davurica Nutrition 0.000 description 1
- 241000675183 Rosa davurica Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 206010048222 Xerosis Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004964 aerogel Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229940069521 aloe extract Drugs 0.000 description 1
- 235000014104 aloe vera supplement Nutrition 0.000 description 1
- 229940035676 analgesics Drugs 0.000 description 1
- 229940035674 anesthetics Drugs 0.000 description 1
- 239000000730 antalgic agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 229960000271 arbutin Drugs 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000003246 corticosteroid Substances 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000006196 drop Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 206010013990 dysuria Diseases 0.000 description 1
- XACKNLSZYYIACO-DJLDLDEBSA-N edoxudine Chemical compound O=C1NC(=O)C(CC)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 XACKNLSZYYIACO-DJLDLDEBSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000003193 general anesthetic agent Substances 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical compound CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000036564 melanin content Effects 0.000 description 1
- 230000003061 melanogenesis Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 229940006093 opthalmologic coloring agent diagnostic Drugs 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000037075 skin appearance Effects 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000003860 topical agent Substances 0.000 description 1
- 229960001727 tretinoin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000006226 wash reagent Substances 0.000 description 1
- 239000003871 white petrolatum Substances 0.000 description 1
- 230000037331 wrinkle reduction Effects 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides application of a wild cherry juice in a composition for inhibiting skin aging, wherein the composition is used for inhibiting melanin generation, inhibiting active oxygen substance generation and promoting skin fibroblast proliferation.
Description
Technical Field
The present application relates to the use of a wild cherry juice. In particular to the application of the wild cherry juice in preparing the composition for inhibiting skin aging.
Background
The skin provides a first stage of protection for human subjects against environmental factors such as Ultraviolet (UV) radiation in the sun, pathogens, friction, and the like. The skin comprises, in order from the outside to the inside, an epidermal layer, a dermal layer mainly composed of connective tissue, and a subcutaneous tissue. Among them, the dermis contains enough molecules such as collagen (collagen), elastin (elastin), and hyaluronic acid (hyaluronic acid), so that the young skin has better support and elasticity.
The skin will age normally with age, resulting in various damaged skin conditions. For example, under the influence of Reactive Oxygen Species (ROS) in the environment, collagen in the skin is cracked, and thus the skin elasticity is reduced, the moisture retention is insufficient (the skin is dry and dull), pores are large, wrinkles and other phenomena are caused; and an aging state such as blotchiness and/or dullness and yellow wax due to melanin precipitation.
However, under certain other environmental factors, the process of skin aging is accelerated. For example, natural factors such as ultraviolet rays in sunlight are generally common. When exposed to ultraviolet rays, the ultraviolet rays can promote skin cells to generate active oxygen substances; in addition, ultraviolet light also induces melanocytes in the skin to produce melanin, which leads to the accelerated production of the aforementioned skin aging states.
In addition, in modern life, the time for people to use electronic products with screens is greatly increased, and visible light (hereinafter referred to as blue light) with shorter wavelength and higher energy emitted by the screens can generate effects similar to ultraviolet rays under the condition of irradiating skin for a long time. Therefore, blue light also becomes a factor that cannot be ignored for skin aging.
Disclosure of Invention
In view of the above, there is a need to develop or research a composition for reducing skin damage caused by uv or blue light to provide skin protection against uv and blue light and further reduce skin aging to maintain or improve skin appearance.
Accordingly, one object of the present invention is to provide an application of a rosa laevigata juice for preparing a composition for resisting skin aging. Specifically, the invention aims to provide the application of the wild cherry juice in preparing the composition for inhibiting free radicals, inhibiting melanin generation and promoting fibroblast growth.
In some embodiments, the composition is used to increase skin elasticity, increase skin moisture, improve skin pore status, reduce skin wrinkles, or inhibit skin wrinkle formation.
In some embodiments, the composition comprises from 2 wt% to 10 wt% of wild cherry juice having a brix of 65-68 ° Bx.
In some embodiments, the composition is a pharmaceutical composition.
In some embodiments, the composition is further prepared as a cosmetic composition, a nutraceutical composition, or a cosmetic composition.
In some embodiments, the composition is an oral composition or a topical composition.
In some embodiments, the content of the Rosa laevigata juice with sugar degree of 65-68 ° Bx in the composition is 0.5 wt%.
As mentioned above, both UV and blue light produce reactive oxygen species in skin cells by similar mechanisms. And because the active oxygen substance has high activity, the active oxygen substance can react with collagen molecules in the skin to further crack the collagen. Since collagen has a "scaffold" in the skin to provide skin with support and elasticity, aging of the skin is accelerated in the absence of collagen, and the skin has decreased elasticity, insufficient water retention (dry and dull skin), enlarged pores, wrinkles, and the like. On the other hand, the ultraviolet rays and the blue light also induce the melanin cells in the skin to generate melanin, so that the skin is speckled and/or dull and yellow, which is one of the common phenomena of skin aging.
The wild cherry juice can effectively prevent various skin aging phenomena caused by ultraviolet rays or blue light and provide protection of the skin against ultraviolet rays and blue light because the wild cherry juice can inhibit the generation of free radicals and melanin.
In addition, the application also proves that the wild cherry juice can promote the growth of the fibroblasts. Since fibroblast can produce collagen, the wild cherry juice can indirectly increase the production of collagen. Therefore, for the collagen, the wild cherry juice not only can passively reduce the loss of the wild cherry juice influenced by free radicals, but also can actively supplement the collagen in the skin, so that the skin state is healthier.
Drawings
FIG. 1 is a graph comparing the results of the cell ratios highly expressed by Reactive Oxygen Species (ROS) for the control blank, the control, and the experimental groups. P value <0.01, compared to control.
FIG. 2 is a graph comparing the results of melanin production in the control group, the control group and the experimental group. P value <0.01, compared to control.
FIG. 3 is a graph comparing the results of cell proliferation rates of the blank control group and the experimental group. P value <0.05, compared to control.
Fig. 4 is a graph comparing changes in skin elasticity of the facial masks of the placebo group and the experimental group after they were applied to the facial skin of the subjects. P value <0.01, compared to baseline. # p value <0.01, compared to placebo.
Fig. 5 is a graph comparing changes in skin moisture retention of the placebo group and the experimental group after the masks were applied to the facial skin of the subjects. P value < 0.01; p value <0.001, compared to baseline. # p value <0.05, compared to placebo.
Fig. 6 is a graph comparing changes in skin pore status of the facial masks of the placebo group and the experimental group after they were applied to the facial skin of the subjects. P value <0.001, compared to baseline.
Fig. 7 and 8 are photographs taken before and after the mask containing the rosa laevigata juice is used by a subject (pores are partially marked by software).
Fig. 9 is a graph comparing changes in skin wrinkles of the facial mask of the placebo group and the experimental group when applied to the facial skin of the subject.
Fig. 10 and 11 are photographs taken before and after the mask containing the rosa laevigata juice is used by the subject (texture part is marked by software).
Fig. 12 is a comparison graph of the skin wrinkle state of the subject before and after the subject drinks the beverage containing the wild cherry juice for 6 weeks continuously.
Fig. 13 and 14 are photographs taken before the subject took the drink (week 0) and after the subject took the drink containing the wild cherry juice (week 6) for 6 consecutive weeks (texture part is marked by software).
Fig. 15 is a comparison graph of pore state of skin of the subject before and after the subject drinks the beverage containing the wild cherry juice for 6 weeks continuously. P value <0.05, compared to week 0.
Fig. 16 and 17 are photographs taken before the subject drinks (week 0) and after the subject drinks the beverage containing the wild cherry juice (week 6) for 6 consecutive weeks (pore part is marked by software).
Fig. 18 is a comparison graph of the skin luster state of the subjects before and after the subjects drink the beverage containing the wild cherry juice for 6 weeks continuously.
Fig. 19 and 20 are photographs taken before the subject drinks (week 0) and after the subject drinks the beverage containing the wild cherry juice (week 6) for 6 consecutive weeks.
Detailed Description
Some embodiments of the present disclosure will be described below. The present disclosure may be embodied in many different forms without departing from the spirit thereof, and the scope of protection should not be limited to the details given herein.
Excel software was used for statistical analysis. Data are expressed as mean ± Standard Deviation (SD) and differences between groups are analyzed by student's t-test (student's t-test).
As used herein, the numerical values are approximate and all experimental data are shown to be within a range of plus or minus 10%, and more preferably within a range of plus or minus 5%.
Prunus serrulata (also known as Black Chokeberry) is a fruit of deciduous shrub of the Rosaceae family.
In some embodiments, the juice extracted from the fruit of prunus davidiana (sometimes referred to as "prunus davidiana juice") inhibits free radical production, inhibits melanin production, and promotes skin fibroblast proliferation. Therefore, the wild cherry juice can be used for preparing the composition for inhibiting the generation of free radicals, inhibiting the generation of melanin and promoting the proliferation of skin fibroblasts.
In some embodiments, the Rosa laevigata juice can effectively prevent various skin aging phenomena caused by ultraviolet rays or blue light, and provide protection for the skin against ultraviolet rays and blue light. In addition, the application also proves that the wild cherry juice can promote the growth of fibroblasts, so the wild cherry juice can actively supplement collagen in the skin.
In some embodiments, the Rosa laevigata juice can also have an effect of improving various conditions or appearances of skin affected by collagen. That is, the wild cherry juice can be, for example: (1) reducing fine lines or wrinkles; (2) reducing the size of skin pores; (3) improving skin thickness, plumpness, and/or elasticity; (4) improving skin color or luster; (5) increasing procollagen production; (6) improving the appearance of skin; or (7) increase the moisture content of the skin. Therefore, the Rosa laevigata juice can be used for preparing a composition for inhibiting and improving skin conditions, wherein the skin conditions can be skin elasticity, skin moisture content, skin pore state, skin wrinkle reduction, skin wrinkle formation inhibition or a combination thereof.
In some embodiments, the composition can be a pharmaceutical composition, a nutraceutical composition, a food composition, or a nutraceutical composition.
The pharmaceutical composition may be formulated into a dosage form suitable for enteral (enterrally), parenteral (parenterally) or topical (topically) administration using techniques well known to those skilled in the art. For example, it may be: injections (injection) [ e.g., sterile aqueous solution (sterile aqueous solution) or dispersion (dispersion) ], sterile powders (sterile powder), external preparations (external preparation), or the like.
The pharmaceutical composition may further comprise a pharmaceutically acceptable carrier (pharmaceutically acceptable carrier) which is widely used in pharmaceutical manufacturing technology. For example, a pharmaceutically acceptable carrier can comprise one or more of the following carriers: emulsifying agents (emulsifying agent), suspending agents (suspending agent), disintegrating agents (disintegrant), disintegrating agents (disintegrating agent), dispersing agents (dispersing agent), binding agents (binding agent), excipients (excipient), stabilizing agents (stabilizing agent), chelating agents (chelating agent), diluting agents (diluent), gelling agents (gelling agent), preservatives (preserving), wetting agents (wetting agent), lubricants (lubricating agent), absorption delaying agents (absorption delaying agent), liposomes (liposome), and the like. The choice and amount of such carriers can be selected by one of skill in the art as appropriate.
In some embodiments, the pharmaceutically acceptable carrier may comprise one of the following solvents: water, normal saline (normal saline), Phosphate Buffered Saline (PBS), aqueous alcohol-containing solution (aqueous solution stabilizing alcohol), and any other suitable solvent.
In some embodiments, the pharmaceutical composition may be administered by any of the parenteral routes (parenteral routes) described below: subcutaneous injection (subeutaneous injection), intraepidermal injection (intraepithelial injection), intradermal injection (intraepithelial injection), and intralesional injection (intralesion).
In some embodiments, the pharmaceutical composition can be manufactured as an external preparation (external preparation) suitable for topical application to the skin using techniques well known to those skilled in the art. For example, it may be any of the following, but is not limited thereto: creams (emulsions), gels (gels), ointments (ointments), creams (creams), patches (patches), liniments (liniments), powders (powders), aerosols (aerogels), sprays (sprays), emulsions (positions), serums (serums), pastes (pastes), foams (foams), drops (drops), suspensions (suspensions), ointments (salves) and bandages (bandages).
In some embodiments, the external preparation is prepared by mixing the pharmaceutical composition with a base (base) as is well known to those skilled in the art.
In some embodiments, the substrate may comprise additives (additives) of one or more of the following: water, alcohols, glycols, hydrocarbons such as petroleum jelly and white petrolatum]Wax (wax) [ such as Paraffin and yellow wax (yellow wax)]Preservatives (preserving agents), antioxidants (antioxidants), surfactants (surfactants), absorption enhancers (absorption enhancers), stabilizers (stabilizing agents), gelling agents (gelling agents) (, ("gelling agents"), ")
Microcrystalline cellulose (microcrystalline cellulose) and carboxymethyl cellulose (carboxymethyl cellulose)]Active agents (actives), moisturizers (humectants), odor absorbers (odor absorbers), perfumes (fragrans), pH adjusting agents (pH adjusting agents), chelating agents (chelating agents), emulsifiers (emulsifiers), occlusive agents (occlusive agents), softeners (emulsifiers), thickeners (thickeners), solubilizing agents (solubilizing agents), penetration enhancers (penetration enhancers), anti-irritants (anti-irritants), colorants (colorants), and propellants (propellants). The selection and amounts of such additives are within the skill and routine skill of those skilled in the art.
In some embodiments, the care product may include an adjuvant (acceptable adjuvant) that is widely used in the art of care product manufacture. For example, the acceptable adjuvant may comprise one or more of the following adjuvants: solvents, gelling agents, active agents, preservatives, antioxidants, screening agents, chelating agents, surfactants, colouring agents, thickening agents, fillers, fragrances and odour absorbers. The selection and amount of these agents can be adjusted according to the actual requirements.
In some embodiments, the cosmetic may be manufactured in a form suitable for skin care (skincare) or makeup (makeup) using techniques well known to those skilled in the art, which may be any one of, but is not limited to: aqueous solutions (aqueous solutions), aqueous-alcoholic solutions (aqueous-alcoholic solutions) or oily solutions (oil solutions), emulsions in the form of oil-in-water type, water-in-oil type or compound type, gels, ointments, creams, masks (masks), patches, liniments, powders, aerosols, sprays, emulsions, slurries, pastes, foams, dispersions, drops, mousses (mousses), sunblocks, lotions (toiletries), foundations (foundations), make-up removal products (make-up removal products), soaps (soaps) and other body cleansing products (body cleansing products), and the like.
In some embodiments, the treatment may also be used in combination with one or more of the following known active topical agents (external use agents): whitening agents (whitening agents) [ such as vitamin a acid (tretinoin), catechin (catechin), kojic acid, arbutin and vitamin C ], moisturizing agents, bactericides (bacteriodes), ultraviolet absorbers (ultravirosomes), plant extracts (plant extracts) [ such as aloe vera extract (aloe extract) ], skin nutrients (skin nutrients), anesthetics (anesthesics), antiacne agents (anti-acne agents), antipruritic agents (antipruritics), analgesics (analgesis), anti-dermatitis agents (anti-dermatitis agents), anti-hypercoagulative agents (anti-xerosis agents), anti-perspiration agents (anti-dysuria agents), anti-aging agents (anti-aging agents), anti-aging agents (anti-seborrheic agents), anti-perspiration agents (anti-aging agents), anti-aging agents (anti-seborrheic agents), and anti-corticosteroid (anti-seborrheic agents). The selection and amounts of such agents for external use are within the skill and routine skill of those skilled in the art.
In some embodiments, the pharmaceutical composition may be used as a food additive (food additive) to be added during the preparation of raw materials by prior art methods or during the manufacture of food products formulated with any edible material for ingestion by humans and non-human animals.
In some embodiments, the type of food product may be, but is not limited to: beverages (leafages), fermented foods (fermented foods), bakery products (bakery products), health foods (health foods) and dietary supplements (dietary supplements).
In some embodiments, the "wild cherry juice" can be juice obtained by squeezing the fruit of wild cherry. For example, in some embodiments, the fruits of prunus wilsonii are ground, squeezed and concentrated to a sugar degree after removing pomace and finer suspended matter. In other embodiments, the aronia melanocarpa juice can be subjected to other processes during the preparation process that do not substantially affect the efficacy of the aronia melanocarpa juice, such as fermentation, to add flavor or other uses.
In some embodiments, the "aronia juice" can also be an extract obtained by extracting the fruit of aronia using a suitable extraction solvent. For example, the fruit extract of the prunus wilsonii can be obtained by soaking prunus wilsonii in water at normal temperature for extraction at a proper time, and then filtering to remove solid impurities.
The experimental procedures in the following examples were carried out at room temperature (25. + -. 5 ℃ C.) and normal pressure (1atm), unless otherwise specified.
[ example 1] cell assay-Rosa laevigata juice inhibits ROS production
In the method, the change of the content of active oxygen substances of human skin fibroblasts CCD-966sk treated by the Rosa laevigata juice is measured by using a fluorescent probe DCFH-DA in combination with a flow cytometer.
Materials and instruments
1. Cell lines: human dermal fibroblast (Human skin fibroblast) CCD-966sk (purchased from biological resource preservation and research center (BCRC), No. 60153).
2. Culture medium: eagle's minimal essential medium (MEM, available from Gibco, 15188-.
3. Phosphate buffered saline (PBS solution): purchased from Gibco, product No. 10437-.
DCFH-DA solution: dichlorodihydrofluorescein diacetate (2, 7-dichoro-dihydro-fluorescein diacetate, DCFH-DA; product number SI-D6883, from Sigma) was dissolved in dimethyl sulfoxide (DMSO, from Sigma, product number SI-D6883-50 MG) to prepare a 5MG/ml solution of DCFH-DA.
5. Flow cytometer (Flow cytometry), Beckman, Catalog No.660519
6. Blue light box (wavelength 400nm-500nm)
7. Wild cherry juice: the fructus Rosae Davuricae juice used in this experiment is juice of fructus Rosae Davuricae, and is available from Lvyuan Gmbycis of Taiwan, with sugar degree of 65-68 ° Bx.
Experimental procedure
The experiment can be divided into three groups, namely an experiment group, a blank control group (a group without adding the wild cherry juice and without blue light irradiation) and a control group (a group without adding the wild cherry juice and with blue light irradiation), wherein three repeated experiments are respectively carried out on each group:
1. CCD-966sk cells were seeded at 1X 105 cells per well in 6-well plates containing 2ml of medium per well.
2. Place the culture dish in 5% CO2And cultured at 37 ℃ for 24 hours.
3. The medium was removed.
4. Experimental groups: adding fructus Rosae Davuricae juice sample 0.625 μ L into each well, and reacting at 37 deg.C for 1 hr. Wherein, the wild cherry juice sample is prepared by diluting the wild cherry juice with water until the sugar degree is 20 ° Bx, and preparing into a solution containing 0.0625 mg of diluted wild cherry juice per ml with culture medium.
Blank control group: 0.625. mu.L of the medium was added to each well and allowed to react at 37 ℃ for 1 hour.
Control group: 0.625. mu.L of the medium was added to each well and allowed to react at 37 ℃ for 1 hour.
5. Add 5 u g/ml DCFH-DA solution 2 u L in each hole, allow it to treat cells for 15 minutes.
6. Experimental groups: the 6-well plate from the DCFH-DA solution treatment step was transferred to a blue light box and allowed to receive blue light exposure for 15 minutes at room temperature (25. + -. 5 ℃).
Blank control group: the 6-well plate in the DCFH-DA solution treatment step was placed in the dark and allowed to stand at room temperature for 15 minutes.
Control group: the 6-well plate from the DCFH-DA solution treatment step was transferred to a blue light box and allowed to receive blue light for 15 minutes at room temperature.
7. After the reaction, the cells were washed 2 times with 1ml of PBS solution per well.
8. Mu.l of Trypsin (Trypsin-EDTA (10X), from Gibco; product No. 15400-. After the reaction, 6mL of the medium solution was added to terminate the reaction.
9. The cells and medium in each well were individually collected into a corresponding 15ml centrifuge tube, and the centrifuge tube containing the cells and medium was centrifuged at 400Xg for 10 minutes.
10. After centrifugation, the supernatant was removed and 1ml of PBS solution was added to mix with the pellet.
11. Centrifuge at 400Xg for 10 min.
12. After centrifugation, the supernatant was removed and the cells were resuspended in 1ml of PBS solution in the dark.
13. The fluorescence signal of DCFH-DA was detected using a flow cytometer. The excitation wavelength for fluorescence detection is 450-490nm, and the emission wavelength is 510-550 nm. Because DCFH-DA enters cells and is hydrolyzed into DCFH (dichlorodihydrofluorescein) and then oxidized into DCF (dichlorofluorescein) capable of emitting green fluorescence by active oxygen substances, the fluorescence intensity of the cells treated by DCFH-DA can reflect the content of the active oxygen substances in the cells, and the proportion of the number of the cells highly expressed by the active oxygen substances in the cells to the number of the original cells is obtained. Since the experiment was performed in duplicate, the results of the duplicate experiments were averaged and are shown in FIG. 1.
Results of the experiment
As shown in FIG. 1, it is clear from the results of the blank control group and the control group that the proportion of cells with high ROS expression (high fluorescence expression) is greatly increased after the blue light irradiation, which indicates that the blue light irradiation actually causes the generation of reactive oxygen species in the cells, and further causes the subsequent damage to the fibroblasts. On the other hand, according to the results of the control group and the experimental group, after the cells are treated by the wild cherry juice, the proportion of the cells with high ROS expression is obviously reduced by about 93 percent compared with the control group, even lower than that of the blank control group. Shows that the wild cherry juice can effectively reduce the generation or accumulation of active oxygen substances in cells, and can be used as an active oxygen substance scavenger. That is, the Rosa laevigata juice can reduce the content of active oxygen substances in cells and reduce the oxidative damage of the cells caused by blue light, ultraviolet rays and the like.
[ example 2] cell experiment-reduction of melanogenesis by Rosa laevigata juice
Herein, the change of melanin content of melanoma cell line B16F10 after treatment with Rosa laevigata juice was determined by ELISA (enzyme-linked immunosorbent assay) reader.
Materials and instruments
1. Cell lines: mouse melanoma cell line B16F10(ATCC CRL-6475) was purchased from American Type Culture Collection (ATCC).
2. Culture medium: dulbecco's modified minor Antibiotic media (DMEM, available from Gibco) was supplemented with additional components to contain 1 vol% Antibiotic solution (Antibiotic solution, available from Gibco, 15240-.
3. Phosphate buffered saline (PBS solution): purchased from Gibco, product No. 10437-.
4. A solution of 1N NaOH (from Sigma, product number 221465) was prepared in double distilled water.
5.ELISA reader(BioTek，FLx 800)
6. Blue light box (wavelength 400nm-500nm)
7. Wild cherry juice: the fructus Rosae Davuricae juice used in this experiment is juice of fructus Rosae Davuricae, and is available from Lvyuan Gmbycis of Taiwan, with sugar degree of 65-68 ° Bx.
Experimental procedure
The experiment can be divided into three groups, namely an experiment group, a blank control group (a group without adding the wild cherry juice and without blue light irradiation) and a control group (a group without adding the wild cherry juice and with blue light irradiation), wherein three repeated experiments are respectively carried out on each group:
1. B16F10 cells were seeded at 1.5 × 105 cells per well in 6-well plates containing 3ml of medium per well.
2. Place the culture dish in 5% CO2And cultured at 37 ℃ for 24 hours.
3. The media was then removed from each well without disturbing the attached cells.
4. Experimental groups: adding 2ml of fructus Rosae Davuricae juice into each well, and culturing at 37 deg.C for 24 hr. Wherein, the wild cherry juice sample is prepared by diluting the purchased wild cherry juice with water to a diluted wild cherry juice with the sugar degree of 20 Bx, and then preparing a solution containing 0.0625 mg of the diluted wild cherry juice per ml by using the culture medium and the diluted wild cherry juice.
Blank control group: 2ml of the medium was added to each well at 37 ℃ and allowed to incubate at 37 ℃ for 24 hours.
Control group: 2ml of the medium was added to each well at 37 ℃ and allowed to incubate at 37 ℃ for 24 hours.
5. Experimental groups: the 6-well plates treated with the aronia melanocarpa medium solution were transferred to a blue light box and exposed to blue light for 3 hours at room temperature (25. + -. 5 ℃).
Blank control group: the 6-well plate was transferred to a dark room and allowed to stand at room temperature for 3 hours.
Control group: the 6 well plates were transferred to a blue light box and allowed to receive blue light irradiation for 3 hours at room temperature.
6. After the reaction, the cells were cultured at 37 ℃ for 48 hours.
7. Then, the medium was removed and the cells were washed 2 times with PBS solution.
8. Mu.l of Trypsin (Trypsin-EDTA (10X), from Gibco; product No. 15400-. After the reaction, the reaction was terminated by adding 6mL of a medium solution. The suspension cells and media in each well were then collected into corresponding 15ml centrifuge tubes, and each centrifuge tube was centrifuged at 400Xg for 5 minutes to pellet the cells.
9. After washing the precipitated cells twice with PBS solution, the cells were resuspended in 200. mu.L of PBS solution.
10. The cell suspension was frozen in liquid nitrogen for 10 minutes and then left at room temperature for about 30 minutes to completely thaw.
11. After complete thawing, the tubes were centrifuged at 12,000g for 3 minutes.
12. The supernatant was removed, and after resuspending the cell pellet with 120. mu.L of 1N NaOH solution, the tube was dried at 60 ℃ for 1 hour to obtain a sample to be assayed.
13. mu.L of the sample to be assayed was transferred to a 96-well plate, and the optical density (OD 450) of the cell solution at 450nm was measured as absorbance using an ELISA plate reader.
Results of the experiment
The relative expression of melanin in the experimental group, the blank control group, and the control group was calculated according to the following formula: relative melanin expression (%) × 100% (OD 450 value of each group/OD 450 value of blank control group). Since the experiment was performed in triplicate, the results of the triplicate experiments were averaged and are shown in FIG. 2.
As shown in fig. 2, it is clear from the results of the blank control group and the control group that the expression amount of melanin in the cells is significantly increased after the blue light irradiation, which indicates that the blue light irradiation indeed induces the melanoma cells to produce melanin, which causes the skin to be blotchy or the skin to be dull. On the other hand, according to the results of the control group and the experimental group, after the cells are treated by the wild cherry juice, the expression amount of melanin under blue light is lower than that of the control group which is not treated by the wild cherry juice (the reduction is about 16%), and the wild cherry juice can effectively reduce the melanin generated by the melanocyte due to the blue light.
[ example 3] cell experiment-Rosa laevigata juice for promoting skin fibroblast proliferation
By means of Click-iTTMThe EdU method (detecting by EdU processing cells, fixing and permeabilizing cells, using a fluorescent dye combined with Edu) detects the cell number, thereby checking whether the wild cherry berry juice has the function of promoting proliferation on fibroblasts.
Materials and instruments
1. Cell lines: human fibroblasts (Human skin fibroblast) CCD-966SK, purchased from American Type Culture Collection (ATCC).
2. Culture medium: eagle's minimal essential medium (MEM, available from Gibco, 15188-.
3. Cell proliferation assay kit (Cell proliferation assay kits, Click-iT)TMPlus EdU Alexa FluorTM488Flow Cytometry Assay Kit), available from Invitrogen, model C10632, 50tests)
The kit comprises the following medicines:
(a) 5-acetylene-2 '-deoxyuridine (5-ethyl-2' -deoxyuridine, EdU)10mg (ingredient A)
(b)Alexa FluorTM488 Picoloazide in DMSO 130. mu.L (component B)
(c) Dimethylsulfoxide (DMSO)4.5mL (component C)
(d)Click-iTTMFixative (Fixative) (4% parafumaldehyde in PBS) 5mL (component D)
(e)Click-iTTMPermeation and washing reagent (fibrous saponin-based perfusion and wash reagent), 10X50 mL (component E)
(f) 0.5mL of an aqueous solution of a Copper protecting agent (Copper protection) at a concentration of 100mM (component F)
(g)Click-iTTMEdU buffer additive (EdU buffer additive)400mg (component G)
4. PBS solution containing 1 vol% BSA (Bovine serum albumin, from Sigma; trade designation A9647), which will be referred to shortly thereafter as 1% BSA/PBS solution.
5.10 mM EdU solution: component C in the kit was taken to be 4mL, added to component A, and stored at-20 ℃.
6. 1X Click-iTTMPermeation and cleaning reagent: component E of the kit was diluted 10-fold with 1% BSA/PBS solution.
7. 10X Click-iTTMEdU buffer additive solution: 2mL of double distilled water was added to component G in the kit and stored at-20 ℃.
8. Phosphate buffered saline (PBS solution): purchased from Gibco, product No. 10437-.
9. Flow cytometer (Flow cytometry), Beckman, Catalog No.660519
10. Wild cherry juice: the fructus Rosae Davuricae juice used in this experiment is juice of fructus Rosae Davuricae, and is available from Lvyuan Gmbycis of Taiwan, with sugar degree of 65-68 ° Bx.
Experimental procedure
The experiment will be divided into two groups of an experiment group and a blank control group (without adding the wild cherry juice):
1. CCD-966sk was seeded at 1X 105 per well in 6-well plates containing 2ml of medium per well in 5% CO2And cultured at 37 ℃ for 24 hours.
2. Experimental groups: adding 2mL of Rosa laevigata juice sample into each well at room temperature, and culturing for 24 hr. Wherein, the wild cherry juice sample is prepared by diluting the purchased wild cherry juice with water to a diluted wild cherry juice with the sugar degree of 20 Bx, and then preparing a solution containing 0.0625 mg of the diluted wild cherry juice per ml by using the culture medium and the diluted wild cherry juice.
Blank control group: 2mL of the medium was added to each well at room temperature and incubated for 24 hours.
3. After the aforementioned 10mM EdU solution was diluted to 10. mu.M with the medium, 2mL of 10. mu.M EdU solution was added to each well and incubated for 2 hours.
4. The cells were collected.
5. Cells were washed once with 1% BSA/PBS solution and the supernatant removed.
6. Adding Click-iT in kitTM100 μ L of fixative (component D) was mixed with the cells in each well and incubated in the dark at room temperature for 15 minutes.
7. Cells were washed once with 1% BSA/PBS solution and the supernatant removed.
8. Addition of 1X Click-iTTM100 μ L of permeation and washing reagent was added to each well and incubated in the dark at room temperature for 15 minutes.
9. Cells were washed once with 1% BSA/PBS solution and the supernatant removed.
10. At 100. mu.L of 1 XClick-iTTMThe reagents were permeabilized and washed, and the cells were resuspended.
11. Carrying out Click-iTTMAnd (4) reacting.
i. Preparation of 1X Click-iTTMEdU buffer additive solution: mixing 10X Click-iTTMThe EdU buffer additive solution is diluted to 1X Click-iT by secondary distilled waterTMEdU buffer additive solution.
Preparation of Click-iTTMPlus reaction mix reagent (required to be used within 15 minutes after preparation)
(Each well of the multi-well plate uses 500. mu.L of Click-iTTMPlus reaction mix reagent of 500. mu.L, 10. mu.L of copper protectant (component F), 2.5. mu.L of Picolinazide DMSO solution (component B), and 50. mu.L of 1 XClick-iTTMEdU buffer additive solution and 438 mu L PBS solution. )
Adding 500 μ L of Click-iT to each compartmentTMPlus reaction mix reagents.
incubate for 30 minutes at room temperature in the dark.
v. as 1X Click-iTTMThe permeabilization and washing reagents wash the cells once and remove the supernatant.
At 500. mu.L of 1X Click-iTTMThe reagents were permeabilized and washed, and the cells were resuspended.
12. Analysis of cells by flow cytometry
Since the dye (component B) will bind specifically to nucleic acids in the cells and produce green fluorescence, and the intensity of the fluorescence will be proportional to the number of cells, the number of cells can be quantified by measuring the fluorescence (excitation wavelength: 488 nm; emission wavelength: 530/30(515-545 nm)).
The cell proliferation rate of the blank control group was used as a reference value (set to 1.0), and the relative cell proliferation rate of the experimental group is shown in fig. 3. As can be seen from FIG. 3, the fibroblasts treated with the Rosa laevigata juice had higher proliferation amount. And because the fibroblasts can produce collagen, the wild cherry juice can indirectly improve the generation amount of the collagen in the skin, so that the skin is firmer and more elastic.
Cell experiments show that the Rosa laevigata juice can actually reduce the generation of free Radicals (ROS) of skin fibroblasts, prevent the skin from being damaged by blue light and ultraviolet rays, inhibit the loss of collagen, and slow down the aging and wrinkle generation caused by the blue light and the ultraviolet rays. And the wild cherry juice can inhibit melanin generation caused by blue light, reduce skin black spot, and maintain white and clean skin. The wild cherry juice can promote the generation of skin fibroblasts, so that the skin is healthier, and finally the effect of resisting skin aging is achieved.
In order to confirm the efficacy of the Rosa laevigata juice for human body, the related human body experiment is also carried out as follows.
Example 4 human experiment-use of wild cherry juice in an external application
The samples used were: comprises a Rosa laevigata juice liquid surface film (prepared by soaking a mask cloth in the essence containing Rosa laevigata juice to be fully absorbed) and a placebo mask (prepared by soaking the same type of mask cloth in the essence, wherein the Rosa laevigata juice is replaced by equal weight of water), and fully absorbing. In this example, each mask contained 20mL of the corresponding essence.
The essence containing the wild cherry juice comprises the following components: 0.2 wt% of xanthan gum, 0.6 wt% of jasminon, 5 wt% of 1, 3-butanediol, 0.1 wt% of triethanolamine, 0.6 wt% of hexanediol, 0.5 wt% of rosa laevigata juice and the balance of water. Wherein the fructus Rosae Davuricae juice is juice of fructus Rosae Davuricae, and is available from Lvyuan Gmbycis of Taiwan, with sugar degree of 65-68 ° Bx.
The number of subjects: 7 subjects between 30-55 years of age.
Experimental mode: after the examinee cleans the whole face, the left and right half faces are recorded before using the facial mask or a photograph is taken before using the facial mask by using corresponding instruments and measuring modes according to different detection items. Then, applying the rose hip juice liquid surface film and the placebo facial film on the right half face and the left half face of the subject respectively, taking down after 15 minutes, and slightly massaging the left half face and the right half face by finger abdomen. After the mask is removed for about 5 minutes, measuring or taking a picture by using a corresponding instrument and a corresponding measuring mode, and comparing the picture with the numerical value of the original left half face or the original right half face (when the mask is to be detected before and after use, the temperature and the humidity of a test area where a subject is located are consistent so as to reduce the influence of factors such as external temperature and humidity on the skin).
Detecting items: the skin will be tested for 1 skin elasticity, 2 skin moisture content, 3 skin pore status, and 4 skin wrinkle status, respectively.
1. Skin elasticity test
A multifunctional skin tester (C + K Multi Probe Adapter System) available from Courage + Khazaka electronics, Germany was used and equipped with
MPA 580 probe. In the test, a probe connected to a skin tester is brought into contact with a region to be tested of the skin of a subject, the region to be tested is sucked by forming a negative pressure on the skin surface, and the change of the sucked depth of the region to be tested with time and the ability of the region to rebound to the original position are measured. The skin elasticity index of the region to be tested can be obtained by analyzing the test result through software attached to the skin elasticity measuring instrument.
As can be seen from fig. 4, compared to the group using placebo facial mask, the skin elasticity can be significantly improved by about 20.8% after applying the facial mask containing rosa laevigata juice. Therefore, the rosa laevigata juice can be applied to the skin in an external mode to improve the elasticity of the skin.
2. Skin Water content test
Skin moisture content was also measured using a multifunctional skin tester, available from Courage + Khazakaelectronic, Germany, in combination with
CM 825 probe. During the test, the probe of the measuring instrument is contacted with the skin area to be measured of the subject, and the moisture content of the epidermal layer of the area to be measured is measured. The method adopts a capacitance method to test the moisture content of human skin, the principle is that the difference of dielectric constants of water and other substances is obvious, the capacitance value of the skin is different according to the difference of the moisture content of the skin, and the observation value can represent the moisture value of the skin.
As can be seen from fig. 5, compared to the group using the placebo facial mask, the moisture content of the skin can be significantly increased by about 25.7% after the facial mask containing the rosa laevigata juice is used. Therefore, when the wild cherry juice is applied to the skin in an external mode, the moisture retention of the skin can be improved.
3. Pore state of skin
The facial skin was examined using a VISIA high-order digital skin texture detector sold by Canfield, usa, and the facial skin was photographed before and after the facial mask was used through a high-resolution camera lens. The method is characterized in that the shadow is generated at the pore dent position by the irradiation of standard white light, the pore is darker than the surrounding skin color, so the pore can be detected, and then a numerical value is obtained by utilizing software according to the analysis of the number and the area of the pore, and the higher the numerical value is, the more the number and the area of the pore are displayed. And the pore part in the print picture of the right half face before and after the liquid surface film containing the wild cherry juice is marked by software.
As can be seen from fig. 6, compared with the use of the mask containing the rosa laevigata juice, the situation of pores in the skin is remarkably improved by about 14.9% after the mask containing the rosa laevigata juice is used, and the number of people who improve the situation of the pores is 100%. It is clear from fig. 7 and 8 that the number of detectable pores is actually decreased after using the mask containing the rosa laevigata juice compared to before using the mask containing the rosa laevigata juice. Therefore, the prunus cerasifera juice is applied to the skin in an external mode, so that the pore condition of the skin can be improved, and the pores are reduced or reduced.
4. Skin wrinkle State test
The VISIA high-order digital skin quality detector is also used for detection, the facial skin before and after the facial mask is used is shot through a high-resolution camera lens, and the texture position can be detected and a numerical value can be obtained by means of standard white light irradiation and detection of the change of the skin shadow, wherein the numerical value can represent the smoothness degree of the skin. And the right half face photos before and after the liquid surface film containing the wild cherry juice are used are marked with the wrinkles by software.
As can be seen from fig. 9, compared with the use of the mask containing the rosa laevigata juice, the wrinkle condition of the skin is remarkably improved by about 9.1% after the mask containing the rosa laevigata juice is used, and the number of people for improving the wrinkle condition of the skin is 100%. As is clear from fig. 10 and 11, the number of skin textures can be reduced compared to the use of the pack containing the rosa laevigata juice before the pack containing the rosa laevigata juice. Therefore, the wild cherry juice is coated on the skin in an external mode, so that the texture of the skin can be reduced, and the smoothness of the skin can be improved.
[ example 5] human body experiment-use of Rosa laevigata juice in an oral administration mode
The samples used were: 50 g/bottle of beverage containing fructus Rosae Davuricae juice (50 g beverage prepared from water and fructus Rosae Davuricae juice contains 1g fructus Rosae Davuricae juice, 2 wt%). In yet other embodiments, up to 5g of wild cherry juice can be ingested per person per day. Wherein the fructus Rosae Davuricae juice is obtained from Rosa Davurica fruit, and has sugar degree of 65-68 ° Bx.
The number of subjects: 8 subjects between 20-60 years of age.
Experimental mode: the subject drunk one bottle of beverage containing wild cherry juice (each bottle contains 1g of wild cherry juice) daily for 42 days (i.e. 6 weeks). Before drinking (face is clean, week 0) and after drinking for 42 days, the corresponding instruments and measuring methods are used to record the numerical value of the facial skin and take pictures before and after drinking according to different detection items. (before and after drinking, the temperature and humidity of the test area where the subject is located are consistent, so as to reduce the influence of factors such as external temperature and humidity on the skin).
Detecting items: skin will be subjected to 1. skin wrinkle state, 2. skin pore state, and 3. skin luster, respectively.
1. Wrinkle state of skin
The detection is carried out by using a VISIA high-order digital skin quality detector sold by Canfield in USA, the facial skin before and after drinking is shot by a high-resolution camera lens, and the texture position can be detected and a numerical value can be obtained by irradiating standard white light and detecting the change of skin shadow, so that the texture position can replace the smoothness degree of the skin. The detection results are shown in FIG. 12; photographs of the face were taken before (week 0) and after (week 6) drinking the beverage containing wild cherry juice and shown in fig. 13 and 14 (wrinkles were marked by software).
As can be seen from fig. 12, after the beverage containing the rosa laevigata juice is continuously drunk for 6 weeks, the wrinkle value of the face skin is obviously reduced by about 8.2% compared with that before the beverage is drunk, and the number of people is improved by 75%. Fig. 13 and 14 clearly show that after drinking the beverage containing the wild cherry juice, the texture quantity of the skin can be reduced and the generation of wrinkles can be inhibited. Shows that the wild cherry juice can reduce the texture of the skin and improve the smoothness of the skin by taking the wild cherry juice orally.
2. Pore state of skin
The skin was also examined using a VISIA high-order digital skin texture detector sold by Canfield, usa, and the facial skin before and after drinking was photographed through a high-resolution camera lens. The method comprises the steps of irradiating standard white light to cause the pits of pores to generate shadows, enabling the pores to be darker than surrounding skin colors, detecting the pores, analyzing by using software according to the number and the area of the pores to obtain a numerical value, and displaying the number and the area of the pores when the numerical value is higher. And the detection results are shown in FIG. 15; photographs of the face before drinking (week 0) and after drinking (week 6) are taken and shown in fig. 16 and 17 (the burr hole portions are marked by software).
As can be seen from fig. 15, after drinking the beverage containing the rosa laevigata juice for 6 weeks, compared with before drinking, the pore condition of the skin is significantly improved by about 12.9%, and the number of people who improve the pore condition reaches 75%. Fig. 16 and 17 clearly show that the number of pores that can be detected is reduced after drinking the beverage containing the wild cherry juice. Shows that the wild cherry juice can also improve the pore condition of skin and reduce or reduce pores in an oral administration way.
3. Skin luster
The facial skin before and after drinking was photographed by a high-resolution camera lens by performing detection using a skin gloss instrument GL 200 purchased from Courage + Khazaka electronic, germany. The reflected light and the scattered light of the skin are measured by the standard white light irradiation, so that the skin glossiness is accurately tested. The detection results are shown in FIG. 18; photographs of the face before and after using the beverage containing the wild cherry juice are shown in fig. 19 and fig. 20.
As can be seen from fig. 18, after the beverage containing the rosa laevigata juice is continuously drunk for 6 weeks, compared with the beverage before drinking, the skin gloss is improved by about 6%, and the improvement rate reaches 75%. Fig. 19 and 20 show that the skin luster can be increased after the beverage containing the wild cherry juice is drunk. Shows that the wild cherry juice can really improve the darkness of the skin and improve the glossiness of the skin in an internal administration mode.
In addition to the above, the present invention also proves that the rosa laevigata juice can promote the growth of fibroblasts, and the fibroblasts can produce collagen, so that the rosa laevigata juice can indirectly increase the amount of collagen produced. Accordingly, the Rosa laevigata juice can be taken orally or externally, so that the aging characteristic of the skin is reduced, the skin is prevented from being damaged by blue light or ultraviolet rays, and the skin aging is inhibited.
The present invention is capable of other embodiments, and various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. Use of Rosa laevigata juice in preparing composition for inhibiting melanin generation is provided.
2. Use of Rosa laevigata juice in preparing composition for inhibiting generation of Reactive Oxygen Species (ROS) is provided.
3. Use of fructus Rosae Davuricae juice in preparing composition for promoting skin fibroblast proliferation is provided.
4. The use according to claim 2 or claim 3, wherein the composition is for increasing skin elasticity, increasing skin moisture content, improving skin pore status, reducing skin wrinkles, inhibiting skin wrinkle formation, or a combination thereof.
5. The use as claimed in any one of claims 1 to 3 wherein the composition comprises from 2 to 10% wt of wild cherry juice having a brix of 65-68 ° Bx.
6. The use of any one of claims 1 to 3, wherein the composition is a pharmaceutical composition.
7. Use according to any one of claims 1 to 3, wherein the composition is further prepared as a maintenance composition, a nutraceutical composition, or a cosmetic composition.
8. The use of any one of claims 1 to 3, wherein the composition is an oral composition.
9. The use as claimed in any one of claims 1 to 3, wherein the composition is a topical composition.
10. The use as claimed in claim 9, wherein the content of the Rosa laevigata juice with sugar degree of 65-68 ° Bx in the composition is 0.5 wt%.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962876014P | 2019-07-19 | 2019-07-19 | |
| US62/876,014 | 2019-07-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112237284A true CN112237284A (en) | 2021-01-19 |
Family
ID=74170547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010695659.9A Pending CN112237284A (en) | 2019-07-19 | 2020-07-17 | Application of wild cherry juice in inhibiting skin aging |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN112237284A (en) |
| TW (1) | TW202103682A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113521136A (en) * | 2021-08-04 | 2021-10-22 | 上海楷达生物科技有限公司 | Composition for skin whitening and wrinkle removal |
| CN114699462A (en) * | 2022-02-24 | 2022-07-05 | 无限极（中国）有限公司 | Composition and application thereof in preparation of product with blue light resisting effect |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011041544A (en) * | 2009-08-24 | 2011-03-03 | Kinshihai Shuzo Kk | Method for producing aronia herb juice |
| JP2013139399A (en) * | 2011-12-28 | 2013-07-18 | Pola Chemical Industries Inc | Melanin production inhibitor |
| US20140227363A1 (en) * | 2011-08-05 | 2014-08-14 | Stemtech International, Inc. | Skin care compositions containing combinations of natural ingredients |
| KR20160088623A (en) * | 2015-01-16 | 2016-07-26 | 현 용 이 | Composition comprising extract of aronia as an active ingredient for preventing and treating aging related disease |
| CN108025195A (en) * | 2015-04-08 | 2018-05-11 | 凯敏工业公司 | Protective action of the lutein to the anti-blue light of human skin cell system |
-
2020
- 2020-07-17 CN CN202010695659.9A patent/CN112237284A/en active Pending
- 2020-07-17 TW TW109124352A patent/TW202103682A/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011041544A (en) * | 2009-08-24 | 2011-03-03 | Kinshihai Shuzo Kk | Method for producing aronia herb juice |
| US20140227363A1 (en) * | 2011-08-05 | 2014-08-14 | Stemtech International, Inc. | Skin care compositions containing combinations of natural ingredients |
| JP2013139399A (en) * | 2011-12-28 | 2013-07-18 | Pola Chemical Industries Inc | Melanin production inhibitor |
| KR20160088623A (en) * | 2015-01-16 | 2016-07-26 | 현 용 이 | Composition comprising extract of aronia as an active ingredient for preventing and treating aging related disease |
| CN108025195A (en) * | 2015-04-08 | 2018-05-11 | 凯敏工业公司 | Protective action of the lutein to the anti-blue light of human skin cell system |
Non-Patent Citations (4)
| Title |
|---|
| 丛峰松: "《神奇的不老莓》", vol. 1, 30 April 2019, 上海交通大学出版社, pages: 33 * |
| 国石磊: "黑果腺肋花楸花色苷分离纯化、结构鉴定及其抗氧化活性研究", pages 024 - 311 * |
| 张永祥等: "黑果腺肋花楸花色苷对人皮肤成纤维细胞紫外辐射损伤的保护作用", 《天然产物研究与开发》, no. 07, 15 July 2016 (2016-07-15), pages 1088 - 1092 * |
| 陈騊声，等: "葡萄酒、果酒与配制酒生产技术", vol. 1, 上海科学技术出版社, pages: 108 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113521136A (en) * | 2021-08-04 | 2021-10-22 | 上海楷达生物科技有限公司 | Composition for skin whitening and wrinkle removal |
| CN114699462A (en) * | 2022-02-24 | 2022-07-05 | 无限极（中国）有限公司 | Composition and application thereof in preparation of product with blue light resisting effect |
Also Published As
| Publication number | Publication date |
|---|---|
| TW202103682A (en) | 2021-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2020519642A (en) | Topical muscadine formulation for beauty use | |
| CN113384507B (en) | Anti-aging composition and preparation method and application thereof | |
| CN108472322A (en) | Composition containing natural extract and their purposes in skin and hair | |
| CN112237284A (en) | Application of wild cherry juice in inhibiting skin aging | |
| TWI809299B (en) | Preparation method of viola mandshurica extract | |
| TW201420107A (en) | Extract of Phalaenopsis amabilis petals, and the preparation process and uses thereof | |
| US20170196797A1 (en) | Composition containing glycine gracilis oil | |
| US7875302B2 (en) | Methods of using grape seed extract to stimulate tyrosinase gene expression | |
| Kim et al. | Anti-oxidation and anti-wrinkling effects of Jeju horse leg bone hydrolysates | |
| TW201420128A (en) | Extract of Phalaenopsis amabilis embryo, and the preparation process and uses thereof | |
| US20220062160A1 (en) | Method for inhibiting skin acne formation, reducing cutibacterium acnes secretions, and/or reducing advanced glycation end-products using wasabia japonica leaf extract | |
| CN112386547B (en) | Skin health care application of pineapple extract | |
| CN114073654A (en) | Use of dewberry juice for preparing skin-conditioning composition | |
| TWI809300B (en) | Use of ilex latifolia thunb extract | |
| TW202106325A (en) | Use ofrosa rugosaextract for manufacturing composition inhibiting skin aging | |
| JP2000319122A (en) | Cell proliferation promoting cosmetic | |
| TWI804771B (en) | Uses of coffee cherry extract for inhibiting and/or reducing the production of collagen glycation end-products | |
| TWI820448B (en) | Use of rubus fruticosus extract for manufacturing a skincare composition | |
| CN114191340A (en) | Use of horseradish leaf juice for improving skin | |
| TWI834368B (en) | Uses of white bayberry extract for resisting aging or reducing fat | |
| WO2024008187A1 (en) | Use of prunus lannesiana extract in preparation of drug for improving skin condition or basal metabolic rate | |
| TWI830382B (en) | Prunus salicina ferment, manufacturing method thereof, and uses thereof | |
| KR102204696B1 (en) | Composition for improving wrinkle comprising 1-monoeicosapentaenoin as effective component | |
| KR20110013238A (en) | Melanogenesis inhibitor and skin-whitening agent | |
| CN113876833A (en) | Mulberry fermented product and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |