CN109044921A

CN109044921A - One kind is anti-ageing to convey nano-lipid vesica and its preparation method and application altogether

Info

Publication number: CN109044921A
Application number: CN201811277092.2A
Authority: CN
Inventors: 刘卫; 洪延涵; 罗丹; 闻庆; 吴航航
Original assignee: Wuhan Bai Si Kay Biotechnology Co Ltd
Current assignee: Wuhan Bai Si Kay Biotechnology Co Ltd
Priority date: 2018-10-30
Filing date: 2018-10-30
Publication date: 2018-12-21

Abstract

The present invention provides a kind of anti-ageing nano-lipid vesicas of conveying altogether and its preparation method and application, belong to effect cosmetic technical field.By weight, the anti-ageing nano-lipid vesica of conveying altogether includes 0.02~32 part of anti-ageing active constituent, 11.1~65 parts of nano-lipid vesica and 3~88 parts of water；The nano-lipid vesica includes the raw material of following parts by weight: 5.0~20.0 parts of nonionic surfactant, 5.0~30.0 parts of water-soluble alcohol, 1.0~10.0 parts and 0.1~5.0 part of cholesterine of composite phospholipid；The anti-ageing active constituent includes water-insoluble aging ingredient and/or water-soluble aging ingredient.The present invention can be realized the transdermal total conveying of the anti-ageing active constituent of a variety of anti-ageing mechanism multiple target points, synergy realized, to improve anti-aging effects by the way that anti-ageing active constituent to be carried in same nano-lipid vesica.

Description

One kind is anti-ageing to convey nano-lipid vesica and its preparation method and application altogether

Technical field

The invention belongs to effect cosmetic fields, and in particular to a kind of anti-ageing conveying nano-lipid vesica and its preparation side altogether Method and application.

Background technique

Skin is most intuitively to characterize at the human body age, and the aging of delaying skin makes skin seem that youth is modern female One of most important skin care needs.The reason of skin aging can be divided into endogenic physiological reason and exogenous environment because Element.The internal cause of physiological dies down with inherent cause, cell ageing, microcirculation, fat is stored up, the variation of hormone in vivo etc. is related, Exogenous environmental factor has ultraviolet light irradiation, environmental pollution etc., in addition, diet and living habit and pressure etc. are also to declining It has a major impact always.

Currently there are many anti-aging cosmetics, but these cosmetics are very different, and that records at present is anti-ageing The generally existing following problems of class cosmetics patented technology:

1. anti-ageing mechanism is single: most anti-ageing class patented invention is only for Antioxidation Mechanism, such as patent CN106265124A In only include two kinds of active ingredients of Cer NS and carnosine, cannot achieve imitate entirely it is anti-ageing；

2. being unable to improve active ingredient stability: most of to have effects that anti-ageing peptide, vitamin and phenols active matter pair The sensitivities such as enzyme, light, pH value are directly appended in formula if handling without nano-package (see patent CN108498445A), Easily lead to unstable active ingredient inactivation；

3. being difficult to ensure anti-ageing active constituent Transdermal absorption: since skin has barrier, anti-ageing active constituent such as active peptide by oneself Etc. being difficult to act on skin care target area through keratoderma, anti-aging function produces little effect.

Therefore, promote active ingredient infiltration, improve stability be anti-ageing class cosmetics realize it is both effectiveness during urgently The problem of solution.

Summary of the invention

In view of this, the present invention provides a kind of anti-ageing nano-lipid vesica of conveying altogether and its preparation method and application, energy The anti-ageing ingredient for enough loading high concentration effectively controls anti-ageing active constituent sustained release, promotes skin permeation amount and improve anti-ageing effect, Realize the synergy of anti-ageing active constituent.

To solve the above-mentioned problems, the present invention provides following technical schemes:

It is a kind of it is anti-ageing convey nano-lipid vesica altogether, by weight, including 0.02~32 part of anti-ageing active constituent, 11.1~65 parts of nano-lipid vesica and 3~88 parts of water；

The nano-lipid vesica includes the raw material of following parts by weight: 5.0~20.0 parts of nonionic surfactant, water-soluble Property 5.0~30.0 parts of alcohol, 1.0~10.0 parts of composite phospholipid and 0.1~5.0 part of cholesterine；

The anti-ageing active constituent includes water-insoluble aging ingredient and/or water-soluble aging ingredient；When anti-ageing work Property ingredient when being water-soluble aging ingredient, the water solubility aging ingredient is encapsulated in the hydrophilic radical folder of nano-lipid vesica In layer；When anti-ageing active constituent is water-insoluble aging ingredient, the water-insoluble aging ingredient is scattered in a nanometer rouge In the interlayer of the hydrophobic grouping of matter vesica.

Preferably, the nonionic surfactant includes: Natrulon H-10 myristinate, -3 methyl of polyglycereol Distearate, Natrulon H-10 oleate, Polyoxyethylene Sorbitan Monooleate, polysorbate -20, -6 laurate of sorbeth, Natrulon H-10 diisopstearate, PEG-15 stearine, Cremophor RH40, laureth -9, C12-14 One of Pareth -12 and C12-14 Pareth -9 are a variety of；

Preferably, the water-soluble alcohol includes ethyl alcohol, hexylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, glycerol, 1,3- Butanediol and 1, one of 2- pentanediol or a variety of；

The composite phospholipid include hydrolecithin, soybean lecithin PC-50, egg yolk lecithin, hydroxylated lecithin and One of hydrogenated lyso lecithin is a variety of.

Preferably, the mass percent of phosphatidyl choline is 25%~30%, 55%~65% in the hydrolecithin Or 75%~85%.

Preferably, the anti-ageing active constituent includes anti-oxidant class active matter, anti-saccharification class active matter, anti-inflammatory agent, matrix At least two in metal protease inhibitors, signal peptide and neurotransmitter peptide for inhibiting.

Preferably, in terms of the parts by weight of anti-ageing active constituent, when active including anti-oxidant class in the anti-ageing active constituent When object, the anti-oxidant class active material is 0.1~10 part；

When in the anti-ageing active constituent including anti-saccharification class active matter, the anti-saccharification class active matter is 0.1~10 Part；

When in the anti-ageing active constituent including anti-inflammatory agent, the anti-inflammatory agent is 0.1~5 part；

When in the anti-ageing active constituent including Matrix Metalloproteinase Inhibitors, the matrix metalloproteinase inhibits Agent is 0.1~5 part；

When in the anti-ageing active constituent including signal peptide, the signal peptide is 0.01~1.0 part；

When in the anti-ageing active constituent including neurotransmitter peptide for inhibiting, the neurotransmitter peptide for inhibiting is 0.01~ 1.0 part.

Preferably, the anti-oxidant class active matter includes vitamin C, vitamin E, astaxanthin, curcumin, carnosine, decarboxylation One of -1 copper of carnosine, dipeptides -4 and tripeptides is a variety of；

The anti-saccharification class active matter include silymarin, pycnogenol, resveratrol, tetrapeptide -30, acetyl group tetrapeptide -5, One of dipeptides -2 and tripeptides -1 are a variety of；

The anti-inflammatory agent includes astaxanthin, Paeoniflorin, celastrin, glycyrrhetinic acid, dipotassium glycyrrhizinate, antibacterial peptide, palm One or both of acyl tetrapeptide -7 and alexin；

The Matrix Metalloproteinase Inhibitors include epigallocatechin gallate, caffeic acid, raspberry, One or both of the water extract honokiol of circle bryony；

The signal peptide includes Matrixyl -3, palmityl tripeptides -1, palmityl hexapeptide, palmityl tripeptides -5, hexapeptide - 9, one of Matrixyl -4, palmityl tetrapeptide -7 and myristoyl pentapeptide -11 or a variety of；

The neurotransmitter peptide for inhibiting include acetyl group hexapeptide -3, acetyl group octapeptide -1, pentapeptide -3, dipeptide ophiotoxin, One of Beta- alanyl hydroxyprolyl- amine and Argireline are a variety of.

The present invention provides the anti-ageing preparation methods for conveying nano-lipid vesica altogether described in a kind of above scheme, including such as Lower step:

1) composite phospholipid, cholesterine, water-insoluble anti-ageing active constituent and nonionic surfactant are mixed, obtains oil Phase；

2) water-soluble anti-ageing active constituent, water-soluble alcohol and water are mixed, obtains water phase；

3) oil of the step 1) mutually and after the mixing of the water phase of the step 2), is successively subjected to emulsification and micronized place Reason, obtains micrometre level dispersoid；

4) micrometre level dispersoid of the step 3) is subjected to nanosizing processing, obtains anti-ageing conveying nano-lipid capsule altogether Bubble；

The step 1) and 2) the not restriction of time sequencing.

Preferably, the mode of micronized processing is shearing in the step 3).

Preferably, in the step 4) nanosizing handle mode include high speed shear, high-pressure homogeneous or high pressure microjet Processing.

The present invention provides anti-ageing conveying nano-lipid vesicas altogether described in a kind of above scheme in preparation anti-aging makeup Application in product.

The present invention provides a kind of anti-ageing nano-lipid vesicas of conveying altogether and its preparation method and application, by weight, Including 0.02~32 part of anti-ageing active constituent, 11.1~65 parts of nano-lipid vesica and 3~88 parts of water；The nano-lipid Vesica includes the raw material of following parts by weight: 5.0~20.0 parts of nonionic surfactant, 5.0~30.0 parts of water-soluble alcohol, compound Phosphatidase 1 .0~10.0 part and 0.1~5.0 part of cholesterine；The anti-ageing active constituent include water-insoluble aging ingredient and/or Water-soluble aging ingredient；When anti-ageing active constituent is water-soluble aging ingredient, the water solubility aging ingredient encapsulating In the hydrophilic radical interlayer of nano-lipid vesica；It is described non-aqueous when anti-ageing active constituent is water-insoluble aging ingredient Dissolubility aging ingredient is scattered in the interlayer of the hydrophobic grouping of nano-lipid vesica.The present invention is by bearing anti-ageing active constituent It is loaded in same nano-lipid vesica, can be realized the transdermal total conveying of the anti-ageing active constituent of a variety of anti-ageing mechanism multiple target points, Synergy is realized, to improve anti-aging effects.Meanwhile solving that water soluble ingredient Transdermal absorption is poor, skin hold-up is low, The disadvantages such as physiological lipid solubility is poor, skin care item additive amount is low, bioavilability is low.Anti-ageing active constituent can effectively penetrate skin Skin, and be detained for a long time in skin, reach instant and long-acting anti-ageing effect.

The anti-ageing nano-lipid vesica of conveying altogether provided by the invention uses nonionic surfactant, water-soluble alcohol, cholesteric Alcohol and composite phospholipid are raw material, are built into nano-lipid vesica in specific proportions, can significantly improve nano-lipid vesica Load capacity forms stable imitated vesicle structure.Embodiment the result shows that: it is provided by the invention it is anti-ageing altogether conveying nano-lipid vesica exist Revolving speed be 15000r/min centrifuge in be centrifuged one hour it is not stratified, without precipitation；Saved at 45 DEG C 3 months it is not stratified, no Change colour, without precipitation；Stored under room temperature 3 years it is not stratified, non-discolouring, stability is good.

After anti-ageing conveying nano-lipid vesica altogether provided by the invention and Skin Cell fusion, phospholipid composition therein can be sent out The effect of waving moisturizing tender skin, makes skin become more soft and glossy.

The anti-ageing nano-lipid vesica of conveying altogether provided by the invention can be used in preparing cosmetics, have cosmetics efficient Advantage anti-ageing, skin sense is excellent, stability is good.Meanwhile the structure of the anti-ageing nano-lipid vesica of conveying altogether provided by the invention is also The natural regeneration function of skin can be excited, accelerate skin metabolism, acted synergistically with anti-ageing active constituent, realized effective Anti-ageing effect.

Further, the present invention is by selecting anti-oxidant class active matter, anti-saccharification class active matter, anti-inflammatory agent, matrix metal At least two in protease inhibitors, signal peptide and neurotransmitter peptide for inhibiting, it is fundamentally removed by more targetings The free radical and inflammatory factor for causing aging prevent aging from cell level, slow down cutis laxa, increase skin elasticity and compact Degree reduces wrinkle and senile plaque, realizes that multiple target point imitates anti-ageing effect entirely.

Detailed description of the invention

Fig. 1 is that the anti-ageing of embodiment 3 conveys nano-lipid vesica transmission electron microscope picture altogether；

Fig. 2 is embodiment 15 to the anti-ageing transdermal performance measurement result figure for conveying nano-lipid vesica altogether；

Fig. 3 is embodiment 15 to the anti-ageing skin cumulative hold-up measurement result figure for conveying nano-lipid vesica altogether；

Fig. 4 is the effect of anti-ageing nano-lipid vesica of conveying altogether of embodiment 18 is to cytoprotection measurement result figure；

Fig. 5 is the anti-ageing nano-lipid vesica of conveying altogether of embodiment 19 to HSF cell SOD effect of vigor result figure；

Fig. 6 is the anti-ageing nano-lipid vesica of conveying altogether of embodiment 20 to HSF cell CAT effect of vigor result figure；

Fig. 7 is anti-ageing shadow of the conveying nano-lipid vesica to reductive glutathione content in HSF cell altogether of embodiment 21 Ring result figure；

Fig. 8 is the result figure that the anti-ageing nano-lipid vesica of conveying altogether of embodiment 22 promotes HSF cell to secrete hyaluronic acid.

Specific embodiment

The present invention provides a kind of anti-ageing conveying nano-lipid vesicas altogether, by weight, including 0.02~32 part resist Decline active constituent, 11.1~65 parts of nano-lipid vesica and 3~88 parts of water；The nano-lipid vesica includes following parts by weight Raw material: 5.0~20.0 parts of nonionic surfactant, 5.0~30.0 parts of water-soluble alcohol, 1.0~10.0 parts of composite phospholipid and 0.1~5.0 part of cholesterine；The anti-ageing active constituent includes water-insoluble aging ingredient and/or water-soluble aging ingredient； When anti-ageing active constituent is water-soluble aging ingredient, the water solubility aging ingredient is encapsulated in the parent of nano-lipid vesica In water base interlayer；When anti-ageing active constituent is water-insoluble aging ingredient, the water-insoluble aging ingredient dispersion In the interlayer of the hydrophobic grouping of nano-lipid vesica.

Anti-ageing convey altogether provided by the invention includes nano-lipid vesica in nano-lipid vesica, by weight, including 11.1~65 parts, preferably 20.5~52 parts, more preferably 28.8~43 parts.

In the present invention, the nano-lipid vesica includes nonionic surfactant, including 5~20 parts, preferably 8~ 17 parts, more preferably 10~15 parts.

In the present invention, the nonionic surfactant preferably includes Natrulon H-10 myristinate, -3 first of polyglycereol Base glucose distearate, Natrulon H-10 oleate, Polyoxyethylene Sorbitan Monooleate, polysorbate -20, -6 laurel of sorbeth Acid esters, Natrulon H-10 diisopstearate, PEG-15 stearine, Cremophor RH40, laureth -9, One of C12-14 Pareth -12 and C12-14 Pareth -9 are a variety of；More preferably Natrulon H-10 nutmeg Acid esters, polyglycereol-3-methyl distearate, Natrulon H-10 oleate, Polyoxyethylene Sorbitan Monooleate, polysorbate -20, - 6 laurate of sorbeth, Natrulon H-10 diisopstearate, PEG-15 stearine, PEG-40 hydrogenated castor Any 2~5 kinds in oil, laureth -9, C12-14 Pareth -12 and C12-14 Pareth -9；Most preferably For Natrulon H-10 myristinate, Natrulon H-10 oleate, -6 laurate of sorbeth and PEG-15 glycerol stearate Ester.The present invention is not particularly limited each component ratio in the nonionic surfactant.The present invention is to the nonionic table The source of face activating agent is not particularly limited, using conventional commercial cosmetics-stage product.

In the present invention, modifying function can be played using nonionic surfactant, lipid vesicle is made to have surface easy The speciality of deformation is more easier to accelerate skin to absorb by skin pore.

In the present invention, the nano-lipid vesica includes water-soluble alcohol, including 5~30 parts, and preferably 10~25 parts, more Preferably 15~20 parts.

In the present invention, the water-soluble alcohol preferably include ethyl alcohol, hexylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, Glycerol, 1,3-BDO and 1, one of 2- pentanediol or a variety of, more preferably ethyl alcohol, hexylene glycol, polyethylene glycol, the third two Alcohol, dipropylene glycol, glycerol, 1,3-BDO and 1,2~3 kinds in 2- pentanediol, most preferably hexylene glycol, propylene glycol, glycerol And 1,3-BDO.The present invention is not particularly limited each component ratio in the water-soluble alcohol.The present invention is to described water-soluble The source of property alcohol is not particularly limited, using conventional commercial cosmetics-stage product.

In the present invention, the water-soluble alcohol can adjust the HLB value of nonionic surfactant boundary layer, to make shape At bilayer structure it is more flexible, imitated vesicle structure is more stable.

In the present invention, the nano-lipid vesica includes composite phospholipid, including 1~10 part, preferably 2~8 parts, more excellent It is selected as 3~7 parts.

In the present invention, the composite phospholipid preferably include hydrolecithin, soybean lecithin PC-50, egg yolk lecithin, One of hydroxylated lecithin and hydrogenated lyso lecithin are a variety of, more preferably hydrolecithin, soybean lecithin PC- 50,1~3 kind in egg yolk lecithin, hydroxylated lecithin and hydrogenated lyso lecithin, most preferably hydrolecithin, soybean Lecithin PC-50 and hydroxylated lecithin.In the present invention, the mass percent of phosphatidyl choline is excellent in the hydrolecithin It is selected as 25%~30%, 55%~65% or 75%~85%, more preferably 55%~65%.The present invention is to the composite phospholipid Mixture in each component ratio be not particularly limited.The present invention is not particularly limited the source of the composite phospholipid, using normal Advise commercially available cosmetics-stage product.

In the present invention, the nano-lipid vesica includes cholesterine, including 0.1~5 part, and preferably 0.5~2 part, more Preferably 0.8~1 part.The present invention is not particularly limited the source of the composite phospholipid, is produced using conventional commercial cosmetics-stage Product.

In the present invention, the cholesterine is adjustable the mobility of lipid vesicle bi-layer membrane, can promote anti-ageing activity Composition is encapsulated and improves its stability.

The anti-ageing nano-lipid vesica of conveying altogether provided by the invention includes anti-ageing active constituent, by weight, including 0.02~32 part, preferably 4.2~17 parts.

In the present invention, the anti-ageing active constituent preferably includes anti-oxidant class active matter, anti-saccharification class active matter, anti-inflammatory At least two in agent, Matrix Metalloproteinase Inhibitors, signal peptide and neurotransmitter peptide for inhibiting.

In the present invention, in terms of the parts by weight of anti-ageing active constituent, when in the anti-ageing active constituent include anti-oxidant class When active matter, the anti-oxidant class active material is preferably 0.1~10 part；More preferably 1~5 part.

In the present invention, the anti-oxidant class active material preferably include vitamin C, vitamin E, astaxanthin, curcumin, One of carnosine, -1 copper of decarboxylation carnosine, dipeptides -4 and tripeptides are a variety of, more preferably include vitamin C, astaxanthin, carnosine and - 1 copper of tripeptides.The present invention is not particularly limited the source of the anti-oxidant class active material, using this field conventional commercial Cosmetic grade product.

In the present invention, the anti-oxidation active substance is by inhibiting free radical to generate and restoring radical chain reaction, clearly Free radical is removed and traps, to reach anti-aging purpose.

In the present invention, in terms of the parts by weight of anti-ageing active constituent, when in the anti-ageing active constituent include anti-saccharification class When active matter, the anti-saccharification class active matter is preferably 0.1~10 part；More preferably 1~5 part.

In the present invention, the anti-saccharification class active material preferably includes silymarin, pycnogenol, resveratrol, tetrapeptide- 30, one of acetyl group tetrapeptide -5, dipeptides -2 and tripeptides -1 or a variety of more preferably include silymarin, resveratrol, four Peptide -30 and dipeptides -2.The present invention is not particularly limited the source of the anti-saccharification class active material, using this field routine city Sell cosmetics-stage product.

In the present invention, the anti-saccharogenic activity ingredient can prevent collagen and the glycan molecule crosslinking of skin corium, from And improves collagen quality, repair impaired skin cell, eliminate blackening, restore elasticity of skin and gloss.

In the present invention, in terms of the parts by weight of anti-ageing active constituent, when in the anti-ageing active constituent including anti-inflammatory agent, The anti-inflammatory agent is preferably 0.1~5 part；More preferably 1~3 part.

In the present invention, the anti-inflammatory agent preferably includes astaxanthin, Paeoniflorin, celastrin, glycyrrhetinic acid, glycyrrhizic acid two One or both of potassium, antibacterial peptide, palmityl tetrapeptide -7 and alexin, more preferably dipotassium glycyrrhizinate, antibacterial peptide and defence Element.The present invention is not particularly limited the source of the anti-inflammatory agent, using this field conventional commercial cosmetics-stage product.

In the present invention, the anti-inflammatory agent can inhibit inflammatory factor IL-1, and the generation of IL-6, IL-8 promote cell again It is raw, it prevents stem cell and stromal fibroblast cells to be divided into the cell for being overexpressed pro-inflammatory cytokine, slows down inflammatory aging Occur.Nano-lipid vesica in cosmetics can promote cell to absorb anti-inflammatory agent, it is made really to be able to contact skin bottom Inflammatory factor through being formed, to play more excellent anti-ageing effect.

In the present invention, in terms of the parts by weight of anti-ageing active constituent, when in the anti-ageing active constituent include matrix metal When protease inhibitors, the Matrix Metalloproteinase Inhibitors is preferably 0.1~5 part；More preferably 1~3 part.

In the present invention, the Matrix Metalloproteinase Inhibitors preferably includes epi-nutgall acid catechin gallic acid One or both of ester, caffeic acid, raspberry, water extract honokiol of circle bryony；More preferably caffeic acid, cover basin Sub- honokiol.The present invention is not particularly limited the source of the Matrix Metalloproteinase Inhibitors, using this field routine Commercially available cosmetics-stage product.

In the present invention, the Matrix Metalloproteinase Inhibitors can be such that the content of MMPs reduces, and induced skin itself produces Damaged tissues are repaired in the degradation of raw inhibitor confrontation collagen, stimulation collagen synthesis, can prevent/slow down aging Occur.

In the present invention, when in the anti-ageing active constituent including signal peptide, the signal peptide is preferably 0.01~1.0 Part；More preferably 0.1~0.5 part.

In the present invention, the signal peptide preferably includes Matrixyl -3, palmityl tripeptides -1, palmityl hexapeptide, palm fibre One of palmitic acid acyl tripeptides -5, hexapeptide -9, Matrixyl -4, palmityl tetrapeptide -7 and myristoyl pentapeptide -11 are a variety of；More Preferably Matrixyl -3, palmityl tripeptides -1 and palmityl tetrapeptide -7.The present invention is to the source of the signal peptide without spy It is different to limit, using this field conventional commercial cosmetics-stage product.

In the present invention, the signal peptide can imitate the repair signal of polypeptide, amplify signal, act on more born of the same parents Extracellular matrix protein (ECM) component generates bigger and more evenly repair, to promote collagen, elastic fibers and transparent Matter acid hyperplasia improves the water content of skin, and reduces microgroove.Hydrophilic peptide in signal peptide generally requires to penetrate into skin bottom Its barrier effect of layer competence exertion, conventional cosmetic directly add hydrophilic peptide and are difficult to permeate it effectively, can not effectively play Effect.The nano-lipid imitated vesicle structure and skin texture that the present invention uses are very much like, can be adsorbed on cuticula, promote parent Effective infiltration of aqueous peptide, to effectively play the Antiageing effect of signal peptide.

In the present invention, when in the anti-ageing active constituent including neurotransmitter peptide for inhibiting, the neurotransmitter inhibits Peptide is preferably 0.01~1.0 part；More preferably 0.1~0.5 part.

In the present invention, the neurotransmitter peptide for inhibiting preferably includes acetyl group hexapeptide -3, acetyl group octapeptide -1, pentapeptide - 3, one of dipeptide ophiotoxin, Beta- alanyl hydroxyprolyl- amine and Argireline or a variety of, more preferably acetyl Base hexapeptide -3, pentapeptide -3 and dipeptide ophiotoxin.The present invention is not particularly limited the source of the neurotransmitter peptide for inhibiting, adopts With this field conventional commercial cosmetics-stage product.

In the present invention, the neurotransmitter peptide for inhibiting can inhibit the synthesis and catecholamine and second of SNARE acceptor The excessive release of acyl gallbladder, local block nerves transmit contraction of muscle signal, make loosening all muscles, reach flat and comfort dynamic line, static line And the effect of microgroove.The nano-lipid vesica that the present invention uses has flexibility, and deformation occurs for energy, hence into cells of superficial layer, directly To muscle layer is entered, the neurotransmitter peptide for inhibiting in nano-lipid vesica is released and is played a role.

In the present invention, the anti-ageing cosmetics include water.By weight, including 3~88 parts, more preferably 40~67 Part.The present invention is not particularly limited the source of the water, using conventional deionized water.In the present invention, the water hair Wave the effect of solvent.

The present invention loads anti-ageing efficacy active component using nano-lipid vesica, is encapsulated in nano-lipid vesica Portion can effectively extend the holding time of anti-oxidation active substance, improve overall stability.Meanwhile, it is capable to realize a variety of anti- The transdermal total conveying of the anti-ageing active constituent of the mechanism that declines multiple target point realizes synergy, to improve anti-aging effects.

The step 1) and 2) the not restriction of time sequencing.

The present invention mixes composite phospholipid, cholesterine, water-insoluble anti-ageing active constituent and nonionic surfactant, obtains To oily phase.

The present invention mixes water-soluble anti-ageing active constituent, water-soluble alcohol and water, obtains water phase.

After obtaining oily phase and water phase, after the present invention mixes the water phase and oil, emulsification and micronized place are successively carried out Reason, obtains micrometre level dispersoid.In the present invention, the mode of the micronized processing is preferably sheared.The revolving speed of the shearing It is preferred that 5000~15000rpm, more preferably 6000~13000rpm；The time of the shearing preferably 1~10min, more preferably 2~5min.

After obtaining micrometre level dispersoid, the micrometre level dispersoid is carried out nanosizing processing by the present invention, is obtained anti-ageing total Convey nano-lipid vesica.In the present invention, the mode of nanosizing processing be preferably high speed shear, high-pressure homogeneous or high pressure Microjet processing.

In the present invention, when using high speed shear processing, the revolving speed of the shearing is preferably 5000~15000rpm, more Preferably 6000~10000rpm；The time of the shearing is preferably 1~10min, more preferably 2~8min.

When using high-pressure homogeneous processing to nanoscale, heating temperature when described high-pressure homogeneous is preferably 25~50 DEG C, More preferably 30~40 DEG C；Pressure when high-pressure homogeneous is preferably 500~1000bar, more preferably 600~800bar；High pressure The number of homogeneous is preferably 1~3 time, and more preferably 2 times.

When being handled using high pressure microjet to nanoscale, feeding temperature is preferably 25~50 DEG C, more preferably 30~40 ℃；Pressure is preferably 60~160MPa, more preferably 100~120MPa；Homogenization cycles are preferably 1~3 time, and more preferably 2 times.

In the present invention, the anti-ageing partial size for conveying nano-lipid vesica altogether being prepared is preferably 10~100nm, More preferably 30~70nm.

In order to further illustrate the present invention, technical solution provided by the invention is retouched in detail below with reference to embodiment It states, but they cannot be interpreted as limiting the scope of the present invention.

Embodiment 1

By mass percentage, by 1% hydrolecithin (phosphatidyl choline 25%~30%), 3.0% soybean lecithin PC-50, cholesterine 0.1%, 2.0% vitamin E, 0.5% decarboxylation carnosine, 2.0% silymarin, 1.0% epi-nutgall acid Catechin gallate and 5% Natrulon H-10 myristinate melt under 50 DEG C of water bath conditions, obtain oily phase, spare；

By 0.5% carnosine, 0.1% astaxanthin, 1.0% Fructus Rubi extract, 0.1%VC, 0.01% Matrixyl -3, 0.01% Argireline, 5%1,3- butanediol, 20% propylene glycol are mixed with the water of surplus, are stirred under 50 DEG C of water bath conditions It mixes, obtains water phase, it is spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 5min under conditions of revolving speed is 5500rpm, Obtain micrometre level dispersoid；

By micrometre level dispersoid revolving speed be 10000rpm under conditions of high speed shearing emulsification 1min, obtain anti-ageing total conveying Nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 98.2nm, PDI 0.213.

Embodiment 2

By mass percentage, by 1.0% hydrolecithin (phosphatidyl choline 55%~65%), 3.0% hydroxylating ovum Phosphatide, 1.0% cholesterine, 2.0% curcumin, -3 methyl of 1.5% resveratrol, 3.0% magnolol and 10.0% polyglycereol Glucose distearate melts under 50 DEG C of water bath conditions, obtains oily phase, spare；

By 2.0% tetrapeptide -30,0.1% palmityl tripeptides -1,1.0% Fructus Rubi extract, 0.1%VC, 0.01% palm Acyl pentapeptide -3,0.05% acetyl group hexapeptide -3,8.0%1,2- pentanediol, 8.0% dipropylene glycol are mixed with the water of surplus, 50 It is stirred under DEG C water bath condition, obtains water phase, it is spare；

By micrometre level dispersoid homogeneous 3 times at 30 DEG C of temperature, homogenization pressure 500bar, anti-ageing total conveying nanometer rouge is obtained Matter vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 89.1nm, PDI 0.205.

Embodiment 3

By mass percentage, by 8% hydrolecithin (phosphatidyl choline 75%~85%), 2% egg yolk lecithin, 5% cholesterine, 1% acetyl group tetrapeptide -5,1% Paeoniflorin, 5% magnolol, 3% Polyoxyethylene Sorbitan Monooleate and 5% polyglycereol - 10 oleates melt under 45 DEG C of water bath conditions, obtain oily phase, spare；

By 1% carnosine, 1% glycyrrhetinic acid, 6% caffeic acid, 1% palmityl hexapeptide, 5% circle bryony water extract, 1% pentapeptide -3,10% ethyl alcohol, 5% hexylene glycol are mixed with the water of surplus, are stirred under 45 DEG C of water bath conditions, and water phase is obtained, standby With；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 7min under conditions of revolving speed is 8000rpm, Obtain micrometre level dispersoid；

By micrometre level dispersoid homogeneous 1 time at temperature 45 C, homogenization pressure 1000bar, anti-ageing total conveying nanometer is obtained Lipid vesicle.The anti-ageing microscopic appearance for conveying nano-lipid vesica altogether is obtained using transmission electron microscope observation, it is specific such as Fig. 1 It is shown.As seen from Figure 1, the anti-ageing nano-lipid vesica of conveying altogether is substantially rounded under transmission electron microscope, and average grain diameter exists 70nm or so, partial size is relatively uniform, while PDI is 0.217.

Embodiment 4

By mass percentage, by 5% hydrogenated lyso lecithin, 5% soybean lecithin PC-50,3% cholesterine, 2% green The trailing plants root of Dahurian angelica, 3% celastrin, 2% polysorbate -20, -6 laurate of 5% sorbeth and 5.0% polyethylene glycol are hard Glycerol melts under 80 DEG C of water bath conditions, obtains oily phase, spare；

By 0.05% Matrixyl -3,0.01% palmityl tripeptides -1,0.1% acetyl group hexapeptide -3,0.02% acetyl Base octapeptide -1,0.5% pentapeptide -3,15%1,3- butanediol, 2%1,2- pentanediol are mixed with the water of surplus, in 40 DEG C of water-bath items It is stirred under part, obtains water phase, it is spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 3min under conditions of revolving speed is 10000rpm, Obtain micrometre level dispersoid；

Using high-speed micro-jet technology, homogeneous 3 times at 50 DEG C, 160Mpa by micrometre level dispersoid are obtained anti-ageing defeated altogether Send nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 79.5nm, PDI 0.313.

Embodiment 5

By mass percentage, by 8% soybean lecithin PC-50,2% hydroxylated lecithin, 4% cholesterine, 10% table Gallocatechin gallate, 3% resveratrol, 5% Natrulon H-10 diisopstearate, 5%PEG-15 glycerol are hard Resin acid ester, 4%PEG-40 hydrogenated castor grease and 6%C12-14 Pareth -9 melt under 50 DEG C of water bath conditions, obtain It is spare to oily phase；

By 1% carnosine, 0.05% palmityl tripeptides -5,0.05% hexapeptide -9,0.1% dipeptide ophiotoxin, 10% poly- second Glycol, 15% propylene glycol, 5% dipropylene glycol are mixed with the water of surplus, are stirred under 35 DEG C of water bath conditions, and water phase is obtained, spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 4min under conditions of revolving speed is 13000rpm, Obtain micrometre level dispersoid；

By micrometre level dispersoid revolving speed be 11000rpm under conditions of high speed shearing emulsification 3min, obtain anti-ageing total conveying Nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 52.4nm, PDI 0.352.

Embodiment 6

By mass percentage, 4% soybean lecithin PC-50,4% egg yolk lecithin, 0.2% cholesterine, 3% dimension are given birth to Plain E, 1% silymarin, 1% carnosine, 5% laureth -9 and 4%C12-14 Pareth -12 are in 45 DEG C of water-bath items It is melted under part, obtains oily phase, it is spare；

By 1% glycyrrhetinic acid, water extract, the 0.5% myristoyl pentapeptide -11,0.1% acetyl group of 2% circle bryony Hexapeptide -8,0.1% Matrixyl -3,10% glycerol and 20% propylene glycol are into 70.8% water, under 85 DEG C of water bath conditions Stirring, obtains water phase, spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 5min under conditions of revolving speed is 8000rpm, Obtain micrometre level dispersoid；

Using high-speed micro-jet technology, it is 50 DEG C, homogeneous 3 times under pressure 60Mpa in temperature setting, obtains anti-ageing total conveying Nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 89.1nm, PDI 0.361.

Embodiment 7

By mass percentage, by 2% hydrolecithin (phosphatidyl choline 55%~65%), 3% soybean lecithin PC- 50,0.4% cholesterine, 1% pycnogenol, 1% celastrin, 1% Paeoniflorin, 3% Natrulon H-10 myristinate and 5% Cremophor RH40 melts under 45 DEG C of water bath conditions, obtains oily phase, spare；

By 1% carnosine, 0.05% hexapeptide -9,0.05% myristoyl pentapeptide -11,5% raspberry water extract, 0.8% Acetyl group hexapeptide -3,0.1% dipeptide ophiotoxin, 10% ethyl alcohol, 15% hexylene glycol are mixed with the water of surplus, in 45 DEG C of water-bath items It is stirred under part, obtains water phase, it is spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 10min under conditions of revolving speed is 8000rpm, Obtain micrometre level dispersoid；

By micrometre level dispersoid homogeneous 3 times at temperature 45 C, homogenization pressure 8000bar, anti-ageing total conveying nanometer is obtained Lipid vesicle.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 91.2nm, PDI 0.274.

Embodiment 8

By mass percentage, by 8% hydroxylated lecithin, 2% hydrogenated lyso lecithin, the dimension life of cholesterine 4%, 2% Plain E, 0.5% decarboxylation carnosine, 2% epigallocatechin gallate and 8%C12-14 Pareth -9 are 50 It is melted under DEG C water bath condition, obtains oily phase, it is spare；

By 4% carnosine, 0.5% astaxanthin, 1.0% tetrapeptide -30,0.1% hexapeptide -9,0.01% Asiaticoside, 2% 1,3-BDO, 3% propylene glycol are mixed with the water of surplus, are stirred under 50 DEG C of water bath conditions, and water phase is obtained, spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 5min under conditions of revolving speed is 10000rpm, Obtain micrometre level dispersoid；

Micrometre level dispersoid is 2 times high-pressure homogeneous under conditions of revolving speed is 600bar, obtain anti-ageing total conveying nanometer rouge Matter vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 63.5nm, PDI 0.302.

Embodiment 9

By mass percentage, by 3% hydroxylated lecithin, 2% hydrolecithin (phosphatidyl choline 75%~85%), Cholesterine 1%, 0.5% decarboxylation carnosine and 4% Natrulon H-10 myristinate melt under 50 DEG C of water bath conditions, obtain oil Phase, it is spare；

By 1% dipotassium glycyrrhizinate, 0.5% Matrixyl -4,1.0%Beta- alanyl hydroxyprolyl- amine, 2%1,3- Butanediol, 3% propylene glycol are mixed with the water of surplus, are stirred under 50 DEG C of water bath conditions, and water phase is obtained, spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification under conditions of revolving speed is 60000rpm 10min obtains micrometre level dispersoid；

By micrometre level dispersoid revolving speed be 6000rpm under conditions of high speed shearing emulsification 10min, obtain anti-ageing total conveying Nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 56.5nm, PDI 0.331.

Embodiment 10

By mass percentage, by 2% hydrogenated lyso lecithin, 2% soybean lecithin PC-50, cholesterine 0.5%, 1% Epigallocatechin gallate and 5%C12-14 Pareth -9 melt under 50 DEG C of water bath conditions, obtain Oily phase, it is spare；

By 0.5% dipeptides -4,0.5% dipeptides -2,1.0% antibacterial peptide, 0.1% Matrixyl -4,0.01% acetyl group Hexapeptide 3,10% propylene glycol, 3% 2 hexylene glycol are mixed with the water of surplus, are stirred under 50 DEG C of water bath conditions, and water phase is obtained, standby With；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 8min under conditions of revolving speed is 70000rpm, Obtain micrometre level dispersoid；

Micrometre level dispersoid is 1 time high-pressure homogeneous under conditions of revolving speed is 1000bar, obtain anti-ageing total conveying nanometer rouge Matter vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 53.17nm, PDI 0.352.

Embodiment 11

By mass percentage, by 2.5% hydrolecithin (phosphatidyl choline 25%~30%), 2% soybean lecithin PC-50, cholesterine 2%, 2% vitamin E and 8% polyglycereol-3-methyl distearate melt under 50 DEG C of water bath conditions Melt, obtains oily phase, it is spare；

By 1% tetrapeptide -30,0.1% hexapeptide -9,0.01% Asiaticoside, 2%1,2- pentanediol, 20% glycerol with it is remaining The water of amount mixes, and stirs under 50 DEG C of water bath conditions, obtains water phase, spare；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 8min under conditions of revolving speed is 80000rpm, Obtain micrometre level dispersoid；

Using high-speed micro-jet technology, homogeneous 3 times at 25 DEG C, 60Mpa by micrometre level dispersoid obtain anti-ageing total conveying Nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 79.20nm, PDI 0.319.

Embodiment 12

By mass percentage, by 8% hydrolecithin (phosphatidyl choline 55%~65%), 2% hydroxylated lecithin, 4% cholesterine and 8% polyglycereol-3-methyl distearate melt under 50 DEG C of water bath conditions, obtain oily phase, spare；

By 1% tripeptides -1,0.5% tetrapeptide -30,1% antibacterial peptide, 0.1% Matrixyl -4,0.01%Beta- propylamine Acyl hydroxyprolyl- amine, 2% ethyl alcohol, 3% hexylene glycol are mixed with the water of surplus, are stirred under 50 DEG C of water bath conditions, and water phase is obtained, standby With；

Water phase and oil are mixed, after the completion of mixing, high speed shearing emulsification 4min under conditions of revolving speed is 90000rpm, Obtain micrometre level dispersoid；

Micrometre level dispersoid is 3 times high-pressure homogeneous under conditions of revolving speed is 800bar, obtain anti-ageing total conveying nanometer rouge Matter vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 61.7nm, PDI 0.323.

Embodiment 13

By mass percentage, by 5% egg yolk lecithin, 5% hydroxylated lecithin, cholesterine 0.5% and 15% month Laureth -9, C12-14 Pareth -12 melt under 50 DEG C of water bath conditions, obtain oily phase, spare；

By -1 copper of 1% tripeptides, 0.5% tripeptides -1,1% alexin, 0.1% Matrixyl -4,5% ethyl alcohol, 23% the third Glycol is mixed with the water of surplus, is stirred under 50 DEG C of water bath conditions, and water phase is obtained, spare；

Using high-speed micro-jet technology, homogeneous 2 times at 25 DEG C, 60Mpa by micrometre level dispersoid obtain anti-ageing total conveying Nano-lipid vesica.

The anti-ageing partial size for conveying nano-lipid vesica altogether is detected, the anti-ageing conveying nano-lipid vesica partial size altogether is obtained For 52.75nm, PDI 0.253.

Embodiment 14

The anti-ageing conveying nano-lipid vesica altogether that embodiment 1~13 is prepared is transferred in closed container, room temperature condition After setting 30 days, 60 days, 90 days, character, partial size and the PDI (polydispersity coefficient) of sample are checked, carrying out detection method (please supplement tool The detection method of body writes a Chinese character in simplified form title if any standard method or with reference to method), specific testing result is as shown in table 1.

Table 1 is anti-ageing to convey nano-lipid Vesicle stability test result altogether

As can be seen from Table 1: anti-ageing conveying nano-lipid vesica soilless sticking, discoloration, lamination altogether provided by the invention, Partial size is between 10~100nm, and the equal < 0.4 of PDI, meets application request.Sample is not also rolled into a ball after placing 90 days Conspicuousness variation does not occur for poly-, discoloration, lamination, sample particle diameter and PDI, still meets practical application request, especially anti- It is still more stable in the highly concentrated situation of the active ingredient that declines, do not find that functional component crystallizes precipitation phenomenon, without anti-ageing active ingredient Leakage, therefore, the anti-ageing nano-lipid vesica of conveying altogether provided by the invention are with good stability.

Embodiment 15

This test is absorbed to the skin of whitening liquid-crystal composition and through performance is verified.

Percutaneous penetration selects improved Franz diffusion cell, with weight for 160~220g male SD rat skin of abdomen For model.Diffusion cell parameter are as follows: effective diffusion area 3.14cm², pool volume 7.0mL is received, receiving liquid is physiological saline, magnetic force Mixing speed is 200rpm.

The rat abdomen skin of intact unbroken is fixed between reception tank and supply pool to (skin inner layer is towards reception Pond)；Acceptance pool is kept for 37.0 ± 0.5 DEG C of constant temperature, draws receiving liquid 0.5mL respectively at 1h, 2h, 4h, 6h, 8h, 10h, 12h, and Supplement release test substance 0.5mL, receiving liquid use 0.22 μm of organic membrane filtration.

Anti-ageing by the preparation of embodiment 3,5,7 conveys nano-lipid vesica and using free carnosine as the compound of control group altogether Anti-ageing composition carries out penetrating absorption as test substance according to the method described above, and concrete outcome is as shown in Figures 2 and 3.

Because in embodiment 3,5,7 preparation it is anti-ageing altogether conveying nano-lipid vesicas containing 1% carnosine, use efficient liquid The concentration of carnosine in phase chromatographic determination receiving liquid, calculates the accumulation transdermal penetration amount at each time point, draw transdermal absorpting medicine-when it is bent Line understands the transdermal test in vitro osmotic effect of carnosine.

Carnosine unit area is calculated as follows accumulates transdermal amount:

Wherein: Q_sTo accumulate transdermal amount；S is effective diffusion area；V is physiological saline volume in reception tank；C_iIt is the 1st time Anti-ageing active ingredient concentration in receiving liquid when to previous sample；N is n-th sample volume；When Cn is the sub-sampling in receiving liquid Anti-ageing active ingredient concentration.

As seen from Figure 2, the 12h Percutaneous permeability of embodiment 3,5,7 and free carnosine is respectively 202.58 μ g/cm²、 187.57μg/cm²、160.53μg/cm²With 350.54 μ g/cm².The skin cumulative transit dose of free carnosine is all apparently higher than implementation The skin cumulative transit dose of example 3,5 and 7 samples.Infiltration capacity is excessively high to be easy to cause active principle to be lost fastly, provided by the invention anti- The total conveying nano-lipid vesica transdermal amount that declines is small, and the anti-ageing active constituent slow release of high concentration can be administered continuously, To improve anti-ageing effect.

As seen from Figure 3,12 hours hold-ups that the embodiment of the present invention 3,5,7 and free carnosine obtain are respectively 23.4 μg/cm²、25.2μg/cm²、21.0μg/cm²With 5.8 μ g/cm².Show anti-ageing conveying nano-lipid vesica altogether provided by the invention It can be improved the skin hold-up of active principle, to provide significantly more anti-ageing effect.

Embodiment 16

Safety evaluatio --- keratinocyte

Keratinocyte (HaCat cell) is a kind of epithelial cell that can synthesize keratoprotein, is the main body of epidermis, can For evaluating the safety of nano-lipid vesica.After Human keratinocytes are digested with trypsin solution, it is inoculated in 96 holes After cell is adherent, anti-ageing conveying nano-lipid vesica altogether prepared by embodiment 3,5,7 is added in plate, anti-ageing to convey nano-lipid altogether Vesica extension rate is 320,160,80,40,20 times, and OD value is surveyed under 450nm wavelength, calculates the survival rate of HaCat cell, tool Body the results are shown in Table 2.

2 HaCat cell survival rate (%) of table

As can be seen from Table 2: when acting on HaCat cell for 24 hours, the anti-ageing total conveying nanometer rouge of the preparation of embodiment 3,5,7 For matter vesica in the range of diluting 20~320 times, the survival rate of HaCat cell is greater than 100%, and no cytotoxicity also has one Fixed rush proliferative capacity.Illustrate the nano-lipid vesica that the present invention is prepared, irritation is not generated to epidermis.

Embodiment 17

Safety evaluatio --- human skin fibroblasts

Human skin fibroblasts (HSF cell) be in dermal layer of the skin main cell and skin aging and cell by Main repair cell after damage.After HSF cell is digested with trypsin solution, DMEM culture medium is added and terminates digestion, after centrifugation Supernatant is abandoned, DMEM complete medium is added, cell is resuspended, be inoculated in 96 orifice plates, rear addition embodiment 3,5,7 prepares anti-ageing total Nano-lipid vesica is conveyed, the anti-ageing nano-lipid vesica extension rate of conveying altogether is 320,160,80,40,20 times, is cultivated respectively Add CCK-8 reagent afterwards for 24 hours, OD value is surveyed under 450nm wavelength, calculate the survival rate of HSF cell, concrete outcome is shown in Table 3.

3 HSF cell survival rate (%) of table

As can be seen from Table 3: anti-ageing conveying nano-lipid vesica altogether prepared by embodiment 3,5,7 is in 20~320 times of dilution In the range of, the survival rate of HSF cell is all larger than 100%, and no cytotoxicity also has certain rush proliferative capacity.Show this It is substantially non-toxic to invent the nano-lipid vesica being prepared, irritation is not generated to epidermis, is that skin cutaneous penetration is more satisfactory It is anti-ageing altogether convey nano-lipid vesica.

Embodiment 18

The anti-ageing nano-lipid vesica of conveying altogether verifies the effect of cytoprotection.

After HSF cell is digested with trypsin solution, DMEM complete medium is added, cell is resuspended, HSF cell is connect For kind after 96 orifice plates, culture for 24 hours, control group adds culture medium, and H is added in model group₂O₂, administration group addition H₂O₂It is added simultaneously free Carnosine, carnosine list contain the anti-ageing nano-lipid vesica of conveying altogether prepared with embodiment 3 and co-culture, wherein free carnosine group, flesh It is identical with the content of carnosine in the anti-ageing nano-lipid vesica of conveying altogether prepared by embodiment 3 that peptide list contains group.Reaction adds afterwards for 24 hours CCK-8 reagent cultivates 2h under the conditions of 37 DEG C, and OD value is surveyed under 450nm wavelength, and concrete outcome is shown in Fig. 4.

As seen from Figure 4, model group HSF cell is through H₂O₂After induction, cell survival rate decline has compared with the control group There is significant difference (P < 0.05).Free carnosine only has protective effect to oxidative damage cell in high concentration (100mg/L).Implement The cytoprotective effect of example 3 is substantially better than free carnosine and carnosine list contains nano-lipid vesica, illustrates multiple efficacies ingredient pair Oxidative damage cell plays synergistic function.

Embodiment 19

Verify influence of the anti-ageing nano-lipid vesica of conveying altogether to HSF cell SOD vigor.

Anti-ageing the effect of conveying nano-lipid vesica scavenging activated oxygen altogether, can be anti-by superoxide dismutase (SOD) It reflects, SOD can remove ultra-oxygen anion free radical, protect cells from damage.After HSF cell is digested with trypsin solution, DMEM culture medium is added and terminates digestion, and is inoculated in 6 orifice plates, after culture for 24 hours, control group adds culture medium, and H is added in model group₂O₂, H is added in administration group₂O₂Free carnosine is added simultaneously, carnosine list contains the anti-ageing conveying nano-lipid vesica altogether prepared with embodiment 3 (wherein dissociate carnosine group, carnosine list contain carnosine in the anti-ageing nano-lipid vesica of conveying altogether prepared by group and embodiment 3 for co-cultivation Content it is identical).After culture for 24 hours, the supernatant of HSF cell is sucked, a certain amount of lysate is directly added in six orifice plates and (covers Cover full cell), SOD vigor is surveyed after cracking, measurement result is shown in Fig. 5.

As seen from Figure 5, be added prolong decline total conveying nano-lipid vesica after (embodiment 3) HSF cell in SOD it is living Power is apparently higher than free carnosine and carnosine list contains nano-lipid vesica；Illustrate that conveying nano-lipid vesica (embodiment 3) exists altogether Antioxidation is stronger under the collective effect of multiple efficacies ingredient.

Embodiment 20

Verify influence of the anti-ageing nano-lipid vesica of conveying altogether to HSF cell CAT vigor.

Catalase (CAT) decomposing H₂O₂Reaction can by be added ammonium molybdate terminate rapidly, remaining H₂O₂With molybdenum Sour ammonium effect generates a kind of flaxen complex compound, its variation is measured at 405nm, can calculate the vigor of CAT.Sample preparation is same Embodiment 19, concrete operation step are illustrated to carry out by CAT kit, and measurement result is shown in Fig. 6.

As seen from Figure 6, be added prolong decline total conveying nano-lipid vesica after (embodiment 5) HSF cell in CAT it is living Power is apparently higher than free carnosine and carnosine list contains nano-lipid vesica；Illustrate altogether conveying nano-lipid vesica multiple efficacies at It is stronger that hydrogen peroxide ability, antioxidation are removed under the collective effect divided.

Embodiment 21

Verify influence of the anti-ageing nano-lipid vesica of conveying altogether to reductive glutathione content in HSF cell.

The main Physiological Function of glutathione GSH is to remove free radical, anti-oxidant, anti-aging.Metabolism produces in body Raw many free radicals meeting damaging cells film, invades life macromolecule, promotes body aging, and induced tumor or Atherosclerosis The generation of change, GSH can eliminate free radical, can play strong protective effect.Sample preparation is the same as embodiment 19, concrete operations step Rapid to carry out by by GSH kit specification, measurement result is shown in Fig. 7.

As seen from Figure 7, be added prolong decline it is total conveying nano-lipid vesica after (embodiment 7) HSF cell in GSH contain Amount is apparently higher than free carnosine and carnosine list contains nano-lipid vesica, and has dose dependent；Illustrate to convey nanometer rouge altogether The various active composition of matter vesica is to anti-oxidant generation synergistic function.

Embodiment 22

The effect of this test promotes HSF cell secretion hyaluronic acid to the anti-ageing nano-lipid vesica of conveying altogether is tested Card.

After HSF cell is digested with trypsin solution, DMEM culture medium is added and terminates digestion, and is inoculated in 24 orifice plates, After culture for 24 hours, control group adds culture medium, and the free carnosine of administration group addition, carnosine list contain anti-ageing defeated altogether with the preparation of embodiment 3 Sending nano-lipid vesica to co-culture, (wherein dissociate carnosine group, carnosine list contain anti-ageing total conveying nanometer prepared by group and embodiment 3 The content of carnosine is identical in lipid vesicle), after culture for 24 hours, the supernatant of HSF cell is collected, supernatant is taken to survey hyalomitome after centrifugation Acid content, concrete outcome are shown in Fig. 8.

As seen from Figure 8: the content for prolonging the secretion hyaluronic acid of the HSF cell after declining total conveying nano-lipid vesica is added Free carnosine is significantly more than added or carnosine list contains the HSF cell of nano-lipid vesica, and there is dose dependent.Illustrate altogether Conveying nano-lipid vesica (embodiment 3) has stronger anti-ageing effect under the collective effect of a variety of anti-ageing functional components.

Embodiment 23

To further illustrate the anti-ageing anti-ageing effect for conveying nano-lipid vesica altogether of the present invention, skin analysis is selected to survey It tries instrument VISIA and skin elasticity tester MPA580 and efficacy test is carried out to sample.

The anti-ageing nano-lipid vesica of conveying altogether that embodiment 1 to the embodiment 13 of each addition 5% is prepared is in blank face In frost, 30 volunteers to the age in 40~one's mid-40s carry out dermatoglyph and flexibility test respectively.Position on probation is face Two cheekbone of portion before test and after continuous use product 4 weeks and 8 weeks, is smeared by the same person with VISIA measurement experiment part Before sample and the dermatoglyph figure of blank group.Concrete outcome see the table below 4:

4 dermatoglyph situation of change of table

Skin elasticity test is carried out to Test sites using skin elasticity tester MPA580, concrete outcome is shown in Table 5:

5 skin elasticity situation of change of table

The anti-aging effects of embodiment 1 to embodiment 13 are significant it can be seen from table 4~5, and skin average surface wrinkle is bright Aobvious to reduce, skin elasticity obviously increases, it is seen that the anti-ageing nano-lipid vesica of conveying altogether of the invention has significant skin anti-ageing Old ability.

To sum up, the anti-ageing nano-lipid vesica of conveying altogether of the invention has low transdermal amount and high hold-up, has Long-acting anti-ageing advantage.

By the above test result it is found that the anti-ageing nano-lipid vesica of conveying altogether provided by the present invention can be good at applying In cosmetic field, and due to its unique lipid vesicle structure, i.e., plays the role of stablizing to active ingredient, be sustained, Product is improved to the Antiageing effect of human skin.

The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims

1. a kind of anti-ageing conveying nano-lipid vesica altogether, which is characterized in that by weight, including 0.02~32 part of anti-ageing work Property ingredient, 11.1~65 parts of nano-lipid vesica and 3~88 parts of water；

The nano-lipid vesica includes the raw material of following parts by weight: 5.0~20.0 parts of nonionic surfactant, water-soluble alcohol 5.0~30.0 parts, 1.0~10.0 parts and 0.1~5.0 part of cholesterine of composite phospholipid；

The anti-ageing active constituent includes water-insoluble aging ingredient and/or water-soluble aging ingredient；When it is anti-ageing activity at When being divided into water-soluble aging ingredient, the water solubility aging ingredient is encapsulated in the hydrophilic radical interlayer of nano-lipid vesica In；When anti-ageing active constituent is water-insoluble aging ingredient, the water-insoluble aging ingredient is scattered in nano-lipid In the interlayer of the hydrophobic grouping of vesica.

2. anti-ageing conveying nano-lipid vesica altogether according to claim 1, which is characterized in that the non-ionic surface active Agent includes: Natrulon H-10 myristinate, polyglycereol-3-methyl distearate, Natrulon H-10 oleate, poly- sorb Alcohol ester -80, polysorbate -20, -6 laurate of sorbeth, Natrulon H-10 diisopstearate, PEG-15 glycerol are hard Resin acid ester, Cremophor RH40, laureth -9, C12-14 Pareth -12 and C12-14 Pareth -9 One of or it is a variety of.

3. anti-ageing conveying nano-lipid vesica altogether according to claim 1, which is characterized in that the water-soluble alcohol includes second Alcohol, hexylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, glycerol, 1,3-BDO and 1, one of 2- pentanediol or a variety of；

The composite phospholipid includes hydrolecithin, soybean lecithin PC-50, egg yolk lecithin, hydroxylated lecithin and hydrogenation One of lysolecithin is a variety of.

4. anti-ageing conveying nano-lipid vesica altogether according to claim 3, which is characterized in that phosphorus in the hydrolecithin The mass percent of phosphatidylcholine is 25%~30%, 55%~65% or 75%~85%.

5. anti-ageing conveying nano-lipid vesica altogether according to claim 1, which is characterized in that the anti-ageing active constituent packet Include anti-oxidant class active matter, anti-saccharification class active matter, anti-inflammatory agent, Matrix Metalloproteinase Inhibitors, signal peptide and neurotransmitter At least two in peptide for inhibiting.

6. anti-ageing conveying nano-lipid vesica altogether according to claim 5, which is characterized in that with the weight of anti-ageing active constituent Part meter is measured, when in the anti-ageing active constituent including anti-oxidant class active matter, the anti-oxidant class active material is 0.1~10 Part；

When in the anti-ageing active constituent including Matrix Metalloproteinase Inhibitors, the Matrix Metalloproteinase Inhibitors is 0.1~5 part；

When in the anti-ageing active constituent including neurotransmitter peptide for inhibiting, the neurotransmitter peptide for inhibiting is 0.01~1.0 Part.

7. anti-ageing conveying nano-lipid vesica altogether according to claim 5 or 6, which is characterized in that the anti-oxidant class is living Property object includes one of vitamin C, vitamin E, astaxanthin, curcumin, carnosine, -1 copper of decarboxylation carnosine, dipeptides -4 and tripeptides Or it is a variety of；

The anti-saccharification class active matter includes silymarin, pycnogenol, resveratrol, tetrapeptide -30, acetyl group tetrapeptide -5, dipeptides - One of 2 and tripeptides -1 are a variety of；

The anti-inflammatory agent includes astaxanthin, Paeoniflorin, celastrin, glycyrrhetinic acid, dipotassium glycyrrhizinate, antibacterial peptide, palmityl four One or both of peptide -7 and alexin；

The Matrix Metalloproteinase Inhibitors includes epigallocatechin gallate, caffeic acid, raspberry, circle open country One or both of water extract honokiol of grape；

The signal peptide includes Matrixyl -3, palmityl tripeptides -1, palmityl hexapeptide, palmityl tripeptides -5, hexapeptide -9, palm fibre One of palmitic acid acyl pentapeptide -4, palmityl tetrapeptide -7 and myristoyl pentapeptide -11 are a variety of；

The neurotransmitter peptide for inhibiting includes acetyl group hexapeptide -3, acetyl group octapeptide -1, pentapeptide -3, dipeptide ophiotoxin, Beta- One of alanyl hydroxyprolyl- amine and Argireline are a variety of.

8. the anti-ageing preparation method for conveying nano-lipid vesica altogether described in claim 1~7 any one, including walk as follows It is rapid:

1) composite phospholipid, cholesterine, water-insoluble anti-ageing active constituent and nonionic surfactant are mixed, obtains oily phase；

3) oil of the step 1) mutually and after the mixing of the water phase of the step 2), is successively carried out emulsification and micronized processing, obtained To micrometre level dispersoid；

4) micrometre level dispersoid of the step 3) is subjected to nanosizing processing, obtains anti-ageing conveying nano-lipid vesica altogether；

The step 1) and 2) the not restriction of time sequencing.

9. preparation method according to claim 8, which is characterized in that the mode of micronized processing is to cut in the step 3) It cuts；

The mode that nanosizing is handled in the step 4) includes high speed shear, high-pressure homogeneous or high pressure microjet is handled.

10. the anti-ageing nano-lipid vesica of conveying altogether is in preparing anti-aging cosmetics described in claim 1~7 any one Using.