CN113350233A - Anti-aging composition and preparation method and application thereof - Google Patents
Anti-aging composition and preparation method and application thereof Download PDFInfo
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- CN113350233A CN113350233A CN202110567788.4A CN202110567788A CN113350233A CN 113350233 A CN113350233 A CN 113350233A CN 202110567788 A CN202110567788 A CN 202110567788A CN 113350233 A CN113350233 A CN 113350233A
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Abstract
The application relates to the field of cosmetics, and particularly discloses an anti-aging composition and a preparation method and application thereof. An antiaging composition comprises Eucommiae cortex, nicotinamide mononucleotide, lecithin, okra polysaccharide, palmitoyl tripeptide-5 and water; the components are prepared by a high-pressure micro-jet method, and the composition is in a nano micro water-in-water emulsion form; the particle size of the nano water-in-micro emulsion is 20-80 nm. The composition can be used for delaying skin aging, and the components of the composition have synergistic effects, so that the composition has the effects of accelerating the elimination of vivotoxin, improving the cell antioxidation, enhancing the cell activity, improving the water content of skin and promoting the synthesis of collagen and elastin in a dermis layer so as to achieve the effects of smoothing wrinkles, firming skin, resisting wrinkles and delaying aging; meanwhile, the nano micro water-in-water emulsion prepared by combining the high-pressure micro jet method has a liposome-like structure, is easier to be absorbed through skin, supplements saccharides and phospholipids on cell membranes, is beneficial to reconstructing barriers of damaged cells and maintains activity.
Description
Technical Field
The application relates to the technical field of cosmetics, in particular to an anti-aging composition and a preparation method and application thereof.
Background
The skin, the largest organ of the human body, is subjected to a complex and cumulative aging process throughout life, similar to other biological components of the human body. The clinical features of skin aging are deep wrinkles, loss of skin elasticity, dryness, laxity, roughness, etc. The aging phenomenon of the skin is mainly caused by endogenous and exogenous factors of the human body, and the external aging is mainly caused by a plurality of factors such as environment, cell oxidation and the like, such as solar radiation, cigarette smoke or other pollution factors, wherein ultraviolet radiation is the main factor of photoaging.
The mechanism of skin aging is very complex. One of them is cell damage caused by various factors including ultraviolet rays and apoptosis increased by the damage, hydrolysis of fibrous components such as collagen caused by increased expression of proteases such as Matrix Metalloproteinases (MMPs), and disruption of fiber bundles caused by increased inflammatory cytokines. Among them, MMPs play a critical role in this process, and MMPs also have various functions such as degradation of extracellular matrix formed of collagen, proteoglycan, elastin, etc., and degradation of proteins expressed on the cell surface, etc. The degradation of MMPs can lead to a reduction and degeneration of extracellular matrix, which is an important factor in the formation of wrinkles, sagging, etc. of the skin. In addition, inflammatory cytokines such as TNF- α, IL-1, and IL-6, which are produced by cells due to inflammation, can cause the formation of skin wrinkles and sagging by inducing the production of MMPs.
Most of anti-aging products in the current market do not consider the influence of transdermal absorption, so that the absorption of effective components is poor, long-acting moisturizing and anti-aging effects are not achieved, and the use experience is poor. There is therefore a strong need for the development of new anti-ageing products.
Disclosure of Invention
In order to improve the transdermal absorption rate of the anti-aging composition and increase the long-acting moisturizing and anti-aging effects, the application provides the anti-aging composition and a preparation method and application thereof.
In a first aspect, the present application provides an anti-aging composition, which adopts the following technical scheme:
an anti-aging composition is prepared from the following raw materials in parts by weight:
the composition is prepared from the following raw materials in parts by weight:
supplementing 100 parts of water, and preparing each component by a high-pressure microjet method, wherein the composition is in the form of a nano water-in-micro emulsion; the particle size of the nano micro water-in-water emulsion is 20-80 nm.
By adopting the technical scheme, because the nicotinamide mononucleotide and the lecithin are used as main anti-aging raw materials, the nicotinamide mononucleotide and the lecithin have certain anti-aging effect, and simultaneously can remove toxins deposited on the skin, stimulate the potential activity of cells, and repair aged and damaged cells of a human body. The eucommia ulmoides is added to enhance the metabolism of skin cell substances and enhance the protective effect of the anti-aging composition on dermal cells; the addition of the okra polysaccharide is beneficial to improving the antioxidation effect of the anti-aging composition on skin, and can accelerate the discharge of toxin in skin, enhance the cell activity and delay the aging of oxidized skin by matching with lecithin; the synthesis of collagen and elastin in the dermis layer can be promoted by adding the palmitoyl tripeptide-5, and the elasticity recovery of the anti-aging composition on the skin is promoted. Therefore, the components have synergistic effect, so that the anti-aging composition has the effects of accelerating the elimination of vivotoxin, improving the antioxidation of cells, enhancing the activity of cells, improving the water content of skin and promoting the synthesis of collagen and elastin in the dermis layer so as to achieve the effects of smoothing wrinkles, tightening the skin, resisting wrinkles and delaying aging. Meanwhile, the nano micro water-in-water emulsion (with the particle size of 20-80 nm) is prepared by combining a high-pressure micro jet technology, so that the transdermal absorption capacity of the product is improved, the product can enter the deep part of the skin, the water content of the skin is improved, the damaged cell barrier can be reconstructed, and the activity of skin cells can be maintained.
Preferably, the composition is prepared from the following raw materials in parts by weight:
water to make up 100 parts.
By adopting the technical scheme, the proportion and the dosage of the eucommia ulmoides, the nicotinamide mononucleotide, the lecithin, the okra polysaccharide and the palmitoyl tripeptide-5 are optimized and adjusted, so that the long-acting moisturizing and anti-aging effects of the anti-aging composition on the skin can be further enhanced.
Preferably, the okra polysaccharide is okra polysaccharide.
Preferably, the nicotinamide mononucleotide is selected from one or a combination of alpha-nicotinamide mononucleotide and beta-nicotinamide mononucleotide.
By adopting the technical scheme, the nicotinamide mononucleotide can help to maintain the interaction effect of cell nucleus and mitochondria so as to help to reduce the degeneration of mitochondria, so that the anti-aging composition compounded by adding the nicotinamide mononucleotide and other raw materials can effectively improve the cell aging condition, and can help to rebuild barriers of damaged cells and improve the activity of the cells.
In a second aspect, the present application provides a method for preparing an anti-aging composition, which adopts the following technical scheme:
a method for preparing an anti-aging composition, comprising the following steps:
1) mixing okra polysaccharide and water according to the formula amount, adding lecithin after dissolving, heating and dispersing at 50-80 ℃, and homogenizing for 5-10 minutes;
2) after the temperature is reduced to below 40 ℃, adding nicotinamide mononucleotide, palmitoyl tripeptide-5 and eucommia bark according to the formula amount, and uniformly stirring to obtain a water-in-water emulsion;
3) adding the water-in-water emulsion obtained in the step 2) into a micro-jet high-pressure homogenizer, pressurizing for many times at the pressure of 900-1500bar, and filtering by using an ultramicro filter membrane with the aperture of 80-100 nm to obtain the anti-aging composition.
By adopting the technical scheme, the nano micro water-in-water emulsion prepared by combining the composition with a high-pressure micro jet method has a liposome-like structure, is easier to be absorbed transdermally, supplements saccharides and phospholipids on cell membranes, is beneficial to the reconstruction of damaged cells and the maintenance of cell activity, has a very stable structure, and is beneficial to the storage stability of the anti-aging composition.
Preferably, in 3), the water-in-water emulsion is treated three times in a micro-jet high-pressure homogenizer in an increasing pressure mode, and the pressure is 900-1000 bar, 1100-1200 bar and 1300-1400 bar in sequence.
Through adopting above-mentioned technical scheme, through the pressurization operation of cubic, pressure increases gradually step by step, and pressure at every turn progressively increases slowly, so help the broken homogeneity of high pressure of each raw materials, reduce the broken inhomogeneous condition appearance between each raw materials of high-pressure microjet in-process.
In a third aspect, the present application provides a cosmetic, which adopts the following technical scheme:
the cosmetic comprises cosmetically acceptable auxiliary materials and the anti-aging composition, wherein the weight ratio of the anti-aging composition to the cosmetically acceptable auxiliary materials is 1 (9-99).
By adopting the technical scheme, the anti-aging composition is added into cosmetics (such as skin care products like cream water-in-water emulsion and color cosmetics like lipstick, eye shadow and foundation make-up liquid) for matching use, so that the overall anti-aging and long-acting moisturizing effects of the cosmetics can be effectively enhanced, and particularly, the problems of various skin water shortage, floating powder blocking powder and heavy metal deposition on the skin surface of the color cosmetics can be effectively solved.
Preferably, the cosmetically acceptable auxiliary materials include a humectant, glycerin, a thickener, ethylhexylglycerin, 1, 2-hexanediol, rose hydrosol, triethanolamine, and water.
Preferably, the humectant is one or a combination of hydroxyethyl urea, ceramide, sodium hyaluronate, beta-glucan, luba oil, sorbitol, betaine or amino acid humectant; the thickening agent is one or more of carbomer, xanthan gum, seaweed gel, Arabic gum and Caesalpinia spinosa gum.
By adopting the technical scheme, the humectant, the glycerin, the thickener, the ethylhexyl glycerin, the 1, 2-hexanediol, the rose hydrosol, the triethanolamine and the water are common auxiliary materials, and the anti-aging composition can promote the skin care and make-up fitting degree of the cosmetics while matching the anti-aging composition to meet the use specification of the cosmetics.
In a fourth aspect, the application provides an anti-aging composition for preparing cosmetics, daily chemicals and anti-aging medicines, wherein the anti-aging composition is added in an amount of 1-10% by weight.
In summary, the present application has the following beneficial effects:
1. the skin care product can be used for delaying skin aging, and the components of the skin care product have synergistic effects, so that the effects of accelerating the elimination of vivotoxin, improving the cell antioxidation, enhancing the cell activity, improving the water content of skin and promoting the synthesis of collagen and elastin in a dermis layer are achieved, and the effects of smoothing wrinkles, tightening the skin, resisting wrinkles and delaying aging are achieved. Meanwhile, the nano micro water-in-water emulsion prepared by combining the high-pressure micro jet method has a liposome-like structure, is easier to be absorbed through skin, supplements saccharides and phospholipids on cell membranes, is beneficial to reconstructing barriers of damaged cells and maintains activity.
2. The anti-aging composition is preferably added into cosmetics or daily chemicals and is matched with other auxiliary materials for use, so that the overall anti-aging and long-acting moisturizing effects of the cosmetics or the daily chemicals can be effectively enhanced.
Detailed Description
The present application will be described in further detail with reference to examples.
The raw materials used in the examples of the present application are all commercially available products, except for the following specific descriptions.
The Eucommiae cortex is selected from Eucommiae cortex extract (brown yellow powder, specification 10:1) with product number of 0252 produced by Xian Baiqing Biotechnology Co., Ltd
The nicotinamide mononucleotide is selected from nicotinamide mononucleotide (98% of effective substance, white powder, 1 kg/bag) produced by Suzhou wheat-wheel biotechnology limited.
The lecithin is soybean lecithin (PC is more than or equal to 10% and yellow solid) with the product number of S30869-25g, which is obtained from Shanghai source leaf biotechnology limited company.
The okra polysaccharide is selected from okra polysaccharide (brown yellow powder, specification of 10:1) produced by Shaanxi Sinot biotechnology limited.
The palmitoyl tripeptide-5 is selected from palmitoyl tripeptide-5 (CAS number: 623172-56-5, purity: 1000ppm, clear to slightly opaque liquid) produced by Siemens Biotech, Inc.
Examples
Example 1: the dosage ratio of the raw materials of the anti-aging composition is shown in table 1, and the preparation method of the anti-aging composition comprises the following steps:
1) mixing okra polysaccharide with deionized water, heating to 50 deg.C for dissolving, adding lecithin, heating to 60 deg.C for dispersing, and homogenizing for 10 hr.
2) After the temperature is reduced to 40 ℃, nicotinamide mononucleotide, palmitoyl tripeptide-5 and eucommia bark are added and stirred uniformly to obtain the water-in-water type emulsion.
3) Adding the water-in-water type emulsion into a micro-jet high-pressure homogenizer, treating for three times by increasing pressure (the pressure is 1000bar, 1200bar and 1400bar in sequence), and filtering with an ultramicro filter membrane with pore diameter of 80nm to obtain the composition with anti-aging effect, wherein the composition is in the form of nano water-in-water type emulsion, and the particle diameter of the nano water-in-water type emulsion is 50 nm.
Example 2: an anti-aging composition, which is different from that of example 1 in that: the dosage and the proportion of the raw materials of the anti-aging composition are different, and the specific reference is made in table 1.
The preparation method of the anti-aging composition comprises the following steps:
1) mixing okra polysaccharide with deionized water, heating to 50 deg.C for dissolving, adding lecithin, heating to 80 deg.C for dispersing, and homogenizing for 5 hr.
2) After the temperature is reduced to 10 ℃, nicotinamide mononucleotide, palmitoyl tripeptide-5 and eucommia bark are added and stirred uniformly to obtain the water-in-water type emulsion.
3) Adding the water-in-water type emulsion into a micro-jet high-pressure homogenizer, treating for three times by increasing pressure (the pressure is 900bar, 1200bar and 1400bar in sequence), and filtering with an ultramicro filter membrane with pore diameter of 80nm to obtain the composition with anti-aging effect, wherein the composition is in the form of nano water-in-water type emulsion, and the particle diameter of the nano water-in-water type emulsion is 20 nm.
Example 3: an anti-aging composition, which is different from that of example 1 in that: the dosage and the proportion of the raw materials of the anti-aging composition are different, and the specific reference is made in table 1.
The preparation method of the anti-aging composition comprises the following steps:
1) mixing okra polysaccharide with deionized water, heating to 40 deg.C for dissolving, adding lecithin, heating to 50 deg.C for dispersing, and homogenizing for 10 hr.
2) After the temperature is reduced to 25 ℃, nicotinamide mononucleotide, palmitoyl tripeptide-5 and eucommia bark are added and stirred uniformly to obtain the water-in-water type emulsion.
3) Adding the water-in-water type emulsion into a micro-jet high-pressure homogenizer, treating for three times by increasing pressure (the pressure is 900bar, 1100bar and 1300bar in sequence), and filtering with an ultramicro filter membrane with aperture of 100nm to obtain the composition with anti-aging effect, wherein the composition is in the form of nano water-in-water type emulsion, and the particle size of the nano water-in-water type emulsion is 80 nm.
Examples 4 to 6: an anti-aging composition, which is different from that of example 1 in that: the dosage and the proportion of the raw materials of the anti-aging composition are different, and the specific reference is made in table 1.
TABLE 1 raw material components and corresponding amounts (g) of antiaging compositions in examples 1-6
Examples 7 to 14: an anti-aging composition, which is different from that of example 1 in that: the dosage and the proportion of the raw materials of the anti-aging composition are different, and the specific reference is made in table 2.
TABLE 2 raw material components and corresponding amounts (g) of antiaging compositions in examples 7-14
Example 15: an antiaging composition which is different from example 14 in that: the nicotinamide mononucleotide is alpha-nicotinamide mononucleotide.
Application example
Application example 1: a cosmetic product comprising the anti-aging composition of example 14 and a cosmetically acceptable adjuvant, the weight ratio of the anti-aging composition to the cosmetically acceptable adjuvant being 1: 9. the cosmetic specifically comprises the following components:
the preparation method comprises the step of uniformly mixing the anti-aging composition of example 14, sodium hyaluronate, glycerin, a thickening agent, ethylhexyl glycerin, 1, 2-hexanediol, rose hydrosol and pure water according to a ratio.
Application example 2: a cosmetic composition which is different from application example 2 in that: the anti-aging composition comprises the anti-aging composition of example 14 and cosmetically acceptable auxiliary materials, wherein the weight ratio of the anti-aging composition to the cosmetically acceptable auxiliary materials is 1: 99.
the cosmetic specifically comprises the following components:
comparative example
Comparative example 1: an antiaging composition which is different from example 14 in that: eucommia ulmoides is not added in the raw materials of the formula.
Comparative example 2: an antiaging composition which is different from example 14 in that: nicotinamide mononucleotide is not added in the raw materials of the formula.
Comparative example 3: an antiaging composition which is different from example 14 in that: lecithin is not added in the raw materials of the formula.
Comparative example 4: an antiaging composition which is different from example 14 in that: the formulation contains only 8 grams of beta-nicotinamide mononucleotide, 4 grams of lecithin and 88 grams of deionized water.
Performance detection analysis
Test one: MTT assay effect of anti-aging compositions on fibroblast viability subjects: examples 1-15 were used as test samples 1-15, and comparative examples 1-4 were used as control samples 1-4.
The test principle is as follows: the MTT method is also called MTT colorimetric method, and is a method for detecting cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT into water-insoluble blue-violet crystalline Formazan (Formazan) and deposit the Formazan in the cells, and dead cells do not have the function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and an enzyme linked immunosorbent assay detector is used for measuring the light absorption value of the formazan at the wavelength of 570nm, so that the quantity of living cells can be indirectly reflected. Within a certain range of cell number, MTT crystals are formed in an amount proportional to the cell number.
Skin cells generate a large amount of Reactive Oxygen Species (ROS) after being stimulated by UVB, and the particles have strong chemical reaction activity due to the existence of unpaired free electrons and comprise superoxide anion and hydrogen peroxide (H)2O2) Hydroxyl radicals, and the like.
The test steps are as follows: cells were as per 4X103The density of each well is inoculated in a 96-well plate, cells are not added in the edge wells, and only sterile PBS is added, so that 6 repeated wells are formed in each experimental group; culturing in an incubator, grouping according to experimental design after cell state is stable after 24h, adding culture medium with corresponding volume to control group and UVB irradiation group, and adding 1g of anti-aging composition of one of examples 1-15 or comparative examples 1-4 to medicine groups 1-5 (i.e. anti-aging compositions 1-5) 2h before UVB irradiation for pretreatment; after 25 minutes of UVB irradiation, the plate was removed from the incubator by replacing fresh medium for 24 hours, and 20. mu.L MTT solution (5mg/ml, i.e., 0.5% MTT) was added to each well, and the incubation was continued for 4 hours. If the drug reacts with MTT, the culture medium can be discarded after centrifugation, and the MTT-containing culture medium can be added after 2-3 times of washing with PBS carefully. The culture was terminated and the culture medium in the wells was carefully aspirated. Add 150. mu.L of dimethyl sulfoxide into each well, and shake for 10min at low speed on a shaking bed to dissolve the crystals sufficiently. The absorbance (OD) of each well was measured at an enzyme linked immunosorbent assay OD490 nm.
TABLE 3 protective Effect of anti-aging compositions on UVB-damaged skin fibroblasts
TABLE 4 protective Effect of the anti-aging compositions of examples 1-15 on UVB-damaged skin fibroblasts
In combination with example 14, the control group and the UVB group, and in combination with tables 1 to 3, it can be seen that the cell death rate of the UVB-irradiated group is approximately 50% compared to the control group, the number of cells increases after the treatment with the anti-aging composition, and the number of cells is proportional to the concentration of the anti-aging composition in the range of 0.1-3ug/ml of the final concentration of the anti-aging composition.
Combining examples 1-15, comparative examples 1-4, control group and UVB group, and combining tables 1-4, it can be seen that the ratio of the OD value of examples 1-15 to the OD value of the control group is above 61.4%, and it can be seen that the cell death rate of examples 1-15 is lower than that of UVB irradiated group, and thus, examples 1-15 all have certain effect of protecting skin fibroblasts from UVB damage. The ratios of comparative examples 1 to 4 are close to the values of the UVB group, and thus it can be seen that comparative examples 1 to 4 have almost no protective effect on the skin.
Combining examples 7-10, the control group and the UVB group, and combining tables 1-4, it can be seen that the protective effect of example 7 on skin fibroblasts is obviously better than that of examples 1-6, and the ratio of the OD value of example 7 to that of the control group reaches 67.9%. Meanwhile, the ratio of example 8 is smaller than that of example 7, and thus it can be seen that the protective effect of example 8 on UVB-damaged skin fibroblasts is reduced after the dosage of each raw material component is halved. Compared with example 7, the ratio of the OD value of example 10 to the OD value of the control group reached 68.4% (higher than that of example 7), while the ratio of example 9 was lower than that of example 7 but higher than that of example 8, so that it was found that the cell death rate was decreased (i.e., the protection of UVB-damaged skin fibroblasts was increased) after increasing the amount of nicotinamide mononucleotide and soybean lecithin; meanwhile, on the basis of the dosage of the embodiment 9, the protective effect of the eucommia ulmoides on UVB-damaged skin fibroblasts is further enhanced in the embodiment 10 after the dosage of the eucommia ulmoides is further increased, so that the eucommia ulmoides can enhance the metabolism of human cell substances, and the effects of resisting oxidation, diminishing inflammation and delaying senescence of the anti-aging composition on human cells are enhanced.
Combining examples 10-15, control and UVB, and tables 1-4, it can be seen that the ratio of OD values of examples 11-12 to the OD value of the control is less than that of example 10 after increasing the formulation dosage of β -nicotinamide mononucleotide or soybean lecithin alone, and thus it can be seen that the more nicotinamide mononucleotide or soybean lecithin is not added, the better. Next, in example 15, α -nicotinamide mononucleotide was selected instead of β -nicotinamide mononucleotide, and it was found that α -nicotinamide mononucleotide also has a certain skin-protecting effect, but the effect of example 15 is inferior to that of example 14. It is known that α -nicotinamide mononucleotide is inferior to β -nicotinamide mononucleotide in effect, and α -nicotinamide mononucleotide has a low content of active ingredients acting on skin, and directly affects the synergistic effect between nicotinamide mononucleotide and eucommia ulmoides, lecithin, okra polysaccharide and palmitoyl tripeptide-5.
And (2) test II: anti-aging composition for H2O2Influence test principle of damaged skin fibroblast MDA: MDA (malondialdehyde) is one of the important products of cell lipid peroxidation, and the degree of cell lipid peroxidation can be reflected by testing MDA, and more MDA indicates more serious cell damage.
The test steps are as follows: well-growing cells were digested at 2X105Density of/well was seeded in 6 well plates; grouping according to experimental design after the cells grow to 70-80%; digesting cells, centrifuging to remove supernatant, and repeatedly freezing and thawing the cells by 400 mu L double distilled water to lyse the cells; the supernatant was centrifuged and assayed according to the MDA kit instructions.
TABLE 5
| Group of | Average value of MDA content (nmol/g) |
| Control group | 7.8 |
| H2O2Group of | 10.2 |
| Example 14 ofAnti-aging composition 1 | 8.0 |
| Anti-aging composition 2 of example 14 | 8.2 |
| Anti-aging composition 3 of example 14 | 8.5 |
| Anti-aging composition 4 of example 14 | 8.7 |
| Anti-aging composition 5 of example 14 | 9.5 |
And (3) test results: the test results are shown in Table 5, H2O2The group had a 28% higher MDA content than the control group, the anti-ageing compositions 1-5 of example 14 all had a higher MDA content than the control group, and anti-ageing composition 5 of example 14 had a 12% higher MDA content than the control group, so H2O2Under the condition of damage, cells are damaged by lipid peroxidation, and the anti-aging composition can improve the antioxidant capacity of the cells and relieve the damage of the cells by lipid peroxidation.
And (3) test III: the principle of the test of the influence of the anti-aging composition liquid on the secretion and degradation functions of the extracellular matrix is as follows: MMPs are collagenases that break down type I, type II, and type III collagens and the extracellular matrix. The expression of MMPs of the fibroblasts is increased under UVB irradiation, and the proteins transcribed into the MMPs degrade collagen, so that the decomposition of extracellular matrix is accelerated to cause skin aging.
The test steps are as follows: fibroblast cells with good cell activity and rapid proliferation are mixed at 2x105The density of each well was inoculated into 6-well plates, and after the cell growth density reached 70% -80%, the cells were divided into control group, UVB group and drug group (i.e., anti-aging composition of example 14) according to the experimental design5). UVB and drug groups were treated with UVB irradiation by discarding the old medium from the dishes and overlaying the irradiation with a small amount of PBS to reduce errors. After UVB irradiation, the control group and the UVB group are incubated with fresh culture medium completely, and the medicine group is incubated with fresh culture medium containing the anti-aging composition;
gene level: total cellular RNA was extracted, total RNA was extracted using a total cellular RNA extraction kit (Amidida), cDNA synthesis was performed using a reverse transcription kit, and the changes in expression levels of mRNAs such as MMP-1, MMP-2, and MMP-9, which were detected by the fluorescent quantitative PCR technique, were shown in Table 6 for the reverse transcribed cDNA.
Protein level: and (3) extracting total cell protein, namely firstly collecting cells, cracking the cells by using RIPA cell lysate, centrifuging to obtain the total cell protein, secondly carrying out SDS-PAGE electrophoresis on the total cell protein, and detecting the expression of the type I collagen and the elastin by Western Blot.
TABLE 6
And (3) test results: the results of the experiments are shown in Table 6, the expression of MMP-1, MMP-2 and MMP-9 of the cells composing the fiber cells under UVB irradiation at the gene level is obviously higher than that of the normal control group, while the expression of MMP-1, MMP-2 and MMP-9 of the cells of the anti-aging composition 5 group in example 14 is lower than that of the UVB irradiation group, but still higher than that of the control group. At the protein level, Western Blot shows that the anti-aging composition can increase the expression of type I collagen and elastin.
To test the efficacy of the anti-aging compositions of the present invention, a clinical trial study was conducted, with the following demonstration of the clinical trial data of trial example 14:
clinical trial 1: skin moisturization test
(1) The test method comprises the following steps: clinical trials respectively solicited 30 volunteers to test the products containing the anti-ageing composition; 30 women (age 25-65 years) of volunteers were divided into control and test groups; the subject uses the sample at the arm mark point as required, and the frequency of product use is 2 times/day; the influence of the anti-aging effect composition on the skin moisturizing effect is evaluated by measuring the moisture content change of the mark points of the test subject by using a multifunctional skin tester MPA10 probe after the product is used for 0 day, 14 days and 30 days respectively.
(2) And (3) test results: as can be seen from table 7, compared with before using the test product, the moisturizing effect of the volunteers is improved after the anti-aging composition 5 of example 14 is used for 14 days, and a test result after the anti-aging composition 5 of example 14 is used for 30 days shows that the moisturizing capability of the volunteers is obviously improved, which indicates that the anti-aging composition of the present application can achieve the moisturizing effect by locking moisture and absorbing external moisture, and can increase the moisture content of the skin to play a good moisturizing role.
TABLE 7
Clinical trial 2: skin anti-wrinkle Effect test
(1) The test method comprises the following steps: 30 healthy female volunteers aged 25 to 50 were recruited, and the facial condition of the testers was detected and recorded. The test requires the experimenter to stop using other skin care products, and the using frequency of the product is 2 times/day; facial wrinkles were measured on volunteers using a VISIA skin tester on days 0, 14, and 28 of product use, respectively.
(2) And (3) test results: from table 8, compared with the test product before use, after 14 days of use, the facial wrinkle area ratio of the volunteers is reduced compared with that before use, and in the test result of 30 days, the facial wrinkle area ratio of the volunteers can be obviously reduced, and is averagely reduced by 30.5% compared with the initial value, which indicates that the anti-aging composition can release collagen and elastin in skin tissues by inhibiting the degradation of skin MMPs, improve skin firmness and has the effect of reducing wrinkles.
TABLE 8
The specific embodiments are merely illustrative of the present application and are not restrictive of the present application, and those skilled in the art can make modifications of the embodiments as required without any inventive contribution thereto after reading the present specification, but only protected by the patent laws within the scope of the claims of the present application.
Claims (10)
1. The anti-aging composition is characterized by being prepared from the following raw materials in parts by weight:
0.01-10 parts of eucommia ulmoides;
0.01-10 parts of nicotinamide mononucleotide;
0.01-20 parts of lecithin;
0.01-10 parts of okra polysaccharide;
50.01-10 parts of palmitoyl tripeptide;
supplementing 100 parts of water, and preparing each component by a high-pressure microjet method, wherein the composition is in the form of a nano water-in-micro emulsion; the particle size of the nano micro water-in-water emulsion is 20-80 nm.
2. The anti-aging composition as claimed in claim 1, wherein the composition is prepared from the following raw materials in parts by weight:
0.8-5 parts of eucommia ulmoides;
0.8-5 parts of nicotinamide mononucleotide;
0.8-10 parts of lecithin;
0.8-5 parts of okra polysaccharide;
50.1-4 parts of palmitoyl tripeptide;
water to make up 100 parts.
3. The anti-aging composition as claimed in claim 1, wherein the okra polysaccharide is okra polysaccharide.
4. The anti-aging composition of claim 1, wherein the nicotinamide mononucleotide is selected from the group consisting of α -nicotinamide mononucleotide and β -nicotinamide mononucleotide.
5. A process for the preparation of an anti-ageing composition according to any one of claims 1 to 4, comprising the steps of:
1) mixing okra polysaccharide and water according to the formula amount, adding lecithin after dissolving, heating and dispersing at 50-80 ℃, and homogenizing for 5-10 minutes;
2) after the temperature is reduced to below 40 ℃, adding nicotinamide mononucleotide, palmitoyl tripeptide-5 and eucommia bark according to the formula amount, and uniformly stirring to obtain a water-in-water emulsion;
3) adding the water-in-water emulsion obtained in the step 2) into a micro-jet high-pressure homogenizer, pressurizing for many times at the pressure of 900-1500bar, and filtering by using an ultramicro filter membrane with the aperture of 80-100 nm to obtain the anti-aging composition.
6. The method for preparing an antiaging composition as claimed in claim 5, wherein in 3), the water-in-water emulsion is treated three times in a microfluidizer at an increasing pressure, and the pressure is 900-1000 bar, 1100-1200 bar and 1300-1400 bar in this order.
7. The cosmetic is characterized by comprising cosmetically acceptable auxiliary materials and the anti-aging composition as claimed in any one of claims 1 to 4, wherein the weight ratio of the anti-aging composition to the cosmetically acceptable auxiliary materials is 1 (9-99).
8. The cosmetic of claim 7, wherein the cosmetically acceptable vehicle comprises moisturizers, glycerin, thickeners, ethylhexyl glycerin, 1, 2-hexanediol, rose hydrosol, triethanolamine, and water.
9. The cosmetic according to claim 8, wherein the humectant is one or more of hydroxyethyl urea, ceramide, sodium hyaluronate, beta-glucan, luba oil, sorbitol, betaine or amino acid humectant; the thickening agent is one or more of carbomer, xanthan gum, seaweed gel, Arabic gum and Caesalpinia spinosa gum.
10. Use of an anti-ageing composition according to any one of claims 1 to 4, in the preparation of cosmetic, household chemical and anti-ageing pharmaceutical products, wherein the anti-ageing composition is added in an amount of 1 to 10% by weight.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115192490A (en) * | 2022-07-20 | 2022-10-18 | 广州睿森生物科技有限公司 | Nano microemulsion NMN composition and preparation method and application thereof |
| CN117598923A (en) * | 2023-11-30 | 2024-02-27 | 名宇(广东)化妆品科技有限公司 | Anti-wrinkle tightening composition and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010032891A1 (en) * | 2008-09-16 | 2010-03-25 | Maim Co., Ltd. | Novel palmitoyltripeptide and anti-wrinkle cosmetic composition containing it |
| US20170151171A1 (en) * | 2015-11-26 | 2017-06-01 | Cosmax USA, Corp. | Cosmetic composition for anti-aging |
| CN110237118A (en) * | 2019-06-06 | 2019-09-17 | 泓博元生命科技（深圳）有限公司 | The composition of niacinamide-containing mononucleotide is in the application of anti-aging drug/health care product |
| CN110251443A (en) * | 2019-07-26 | 2019-09-20 | 广州澳希亚实业有限公司 | A kind of skin care item anti-aging Essence and its anti-aging skin care product of application |
| CN110638722A (en) * | 2019-10-31 | 2020-01-03 | 名宇生命科技研发(广州)有限公司 | Anti-aging cosmetic and preparation method thereof |
| CN111374906A (en) * | 2020-03-16 | 2020-07-07 | 嘉文丽（福建）化妆品有限公司 | Composition with anti-aging effect and preparation method thereof |
-
2021
- 2021-05-24 CN CN202110567788.4A patent/CN113350233A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010032891A1 (en) * | 2008-09-16 | 2010-03-25 | Maim Co., Ltd. | Novel palmitoyltripeptide and anti-wrinkle cosmetic composition containing it |
| US20170151171A1 (en) * | 2015-11-26 | 2017-06-01 | Cosmax USA, Corp. | Cosmetic composition for anti-aging |
| CN110237118A (en) * | 2019-06-06 | 2019-09-17 | 泓博元生命科技（深圳）有限公司 | The composition of niacinamide-containing mononucleotide is in the application of anti-aging drug/health care product |
| CN110251443A (en) * | 2019-07-26 | 2019-09-20 | 广州澳希亚实业有限公司 | A kind of skin care item anti-aging Essence and its anti-aging skin care product of application |
| CN110638722A (en) * | 2019-10-31 | 2020-01-03 | 名宇生命科技研发(广州)有限公司 | Anti-aging cosmetic and preparation method thereof |
| CN111374906A (en) * | 2020-03-16 | 2020-07-07 | 嘉文丽（福建）化妆品有限公司 | Composition with anti-aging effect and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115192490A (en) * | 2022-07-20 | 2022-10-18 | 广州睿森生物科技有限公司 | Nano microemulsion NMN composition and preparation method and application thereof |
| CN117598923A (en) * | 2023-11-30 | 2024-02-27 | 名宇(广东)化妆品科技有限公司 | Anti-wrinkle tightening composition and preparation method thereof |
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