CN113528287A - Sample processing device and PCR instrument - Google Patents
Sample processing device and PCR instrument Download PDFInfo
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- CN113528287A CN113528287A CN202010299980.5A CN202010299980A CN113528287A CN 113528287 A CN113528287 A CN 113528287A CN 202010299980 A CN202010299980 A CN 202010299980A CN 113528287 A CN113528287 A CN 113528287A
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- 238000012545 processing Methods 0.000 title claims abstract description 69
- 230000007246 mechanism Effects 0.000 claims abstract description 146
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 71
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 238000012546 transfer Methods 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims description 25
- 230000006698 induction Effects 0.000 claims description 16
- 238000004891 communication Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 4
- 230000026683 transduction Effects 0.000 claims description 3
- 238000010361 transduction Methods 0.000 claims description 3
- 230000005693 optoelectronics Effects 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 abstract description 15
- 102000039446 nucleic acids Human genes 0.000 abstract description 15
- 108020004707 nucleic acids Proteins 0.000 abstract description 15
- 238000011282 treatment Methods 0.000 abstract description 14
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 239000002699 waste material Substances 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000003752 polymerase chain reaction Methods 0.000 description 14
- 239000011324 bead Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
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- 230000009286 beneficial effect Effects 0.000 description 2
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- 239000000463 material Substances 0.000 description 2
- 125000003003 spiro group Chemical group 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
Abstract
The invention relates to the technical field of molecular detection, in particular to a sample processing device and a PCR instrument. The sample processing device comprises a card box, a first liquid transferring valve and an operating mechanism; the card box forms a plurality of reagent chambers, the first pipetting valve is movably connected with the card box, and when the first pipetting valve moves, the first pipetting valve can be respectively communicated with the plurality of reagent chambers; the operating mechanism can be connected with the first pipetting valve to drive the first pipetting valve to move, so that the first pipetting valve is respectively communicated with the plurality of reagent chambers, and the reagent is transferred between the first pipetting valve and the communicated reagent chambers; thereby make first pipetting valve under operating device's drive, transfer the reagent in the treatment solution cavity to the sample treatment cavity in proper order to transfer the waste liquid that produces after the reaction of every step is accomplished in the sample treatment cavity to the waste liquid cavity, and then conveniently obtain nucleic acid solution fast.
Description
Technical Field
The invention relates to the technical field of molecular detection, in particular to a sample processing device and a Polymerase Chain Reaction (PCR) instrument.
Background
With the transformation of medical treatment modes and the continuous development of individual medicines, molecular detection exerts unique advantages; in the molecular detection of a sample, a series of treatments are required to be performed on the sample to obtain a nucleic acid extract. For example, in the sample processing process, multiple reagent transfers are required, and in the prior art, a manual transfer mode is mostly adopted; obviously, the manual material transferring mode is complex in operation, time-consuming and labor-consuming; meanwhile, the materials are difficult to transfer fully and efficiently, and the sample is easy to be polluted, so that the experimental result is influenced.
Disclosure of Invention
The present invention provides a sample processing apparatus and a PCR instrument, which can rapidly and efficiently complete reagent transfer in each step in a sample processing process of molecular detection.
The invention provides a sample processing device, which comprises a card box, a first liquid transferring valve and an operating mechanism, wherein the first liquid transferring valve is arranged on the card box; the cartridge body forms a plurality of reagent chambers, the first pipetting valve being movably connected to the cartridge; the operating mechanism can be connected with the first pipetting valve, and the operating mechanism can drive the first pipetting valve to move so as to enable the first pipetting valve to be respectively communicated with the plurality of reagent chambers and enable the first pipetting valve to be communicated with the reagent chambers to carry out reagent transfer.
Further, the first pipetting valve comprises a first valve body and a first valve rod arranged in the first valve body and extending along a first direction; the first valve body is rotatably connected with the card box, and the first pipetting valve can rotate by different angles so as to be respectively communicated with the plurality of reagent chambers; pushing and pulling the first valve rod to discharge the liquid in the first liquid transferring valve or extract the liquid into the first liquid transferring valve; the operating mechanism comprises a first rotating mechanism and a first push-pull mechanism; the first rotating mechanism can be connected with the valve body of the first liquid transferring valve so as to drive the valve body of the first liquid transferring valve to rotate; the first push-pull mechanism can be connected with the first valve rod to drive the valve rod of the first liquid transferring valve to reciprocate along the first direction.
Further, the test tube rack also comprises a second pipetting valve and a test tube rack; the card box is provided with a redissolution chamber, the test tube rack is arranged on the card box, and a plurality of test tubes are arranged on the test tube rack; the second pipetting valve comprises a second valve body and a second valve rod which is arranged in the second valve body and extends along the first direction; the second valve body is rotatably connected with the card box and can rotate by different angles so as to enable the second pipetting valve to be respectively communicated with the first pipetting valve, the redissolution chamber and the plurality of test tubes; pushing and pulling the second valve rod to discharge the liquid in the second liquid transferring valve or extract the liquid into the second liquid transferring valve; the operating mechanism comprises a second rotating mechanism and a second push-pull mechanism; the second rotating mechanism can be connected with the second valve body so as to drive the second valve body to rotate; the second push-pull mechanism can be connected with the second valve rod to drive the second valve rod to reciprocate along the first direction.
Further, a frame is included; the card box is placed on an installation bearing platform of the frame; the operating mechanism comprises a first mounting plate, the first mounting plate and the mounting bearing platform are oppositely arranged along the first direction at intervals, and the clamping box is positioned between the mounting bearing platform and the first mounting plate; the first rotating mechanism and the second rotating mechanism are positioned on the first mounting plate, and both the first rotating mechanism and the second rotating mechanism comprise a connecting shaft, a rotation driving device, a main gear and a pinion; the connecting shaft is arranged along the first direction and is rotatably connected with the first mounting plate; the fixed end of the rotary driving device is connected with the first mounting plate, and the movable end of the rotary driving device is connected with the main gear so as to drive the main gear to rotate; the auxiliary gear is sleeved on the connecting shaft and meshed with the main gear; the connecting shaft of the first rotating mechanism can be connected with the first valve body, and the connecting shaft of the second rotating mechanism can be connected with the second valve body.
Further, the first rotating mechanism and the second rotating mechanism both comprise a first photoelectric switch and a first induction sheet; the first photoelectric switch is arranged on the first mounting plate and is in communication connection with the rotary driving device so as to control the start and stop of the rotary driving device; the first sensing piece is connected with the main gear and can rotate along with the main gear; a plurality of openings are formed in the edge part of the first induction sheet and are distributed at intervals in the circumference; the plurality of openings on the first sensing piece of the first rotating mechanism correspond to the plurality of reagent chambers and the second pipetting valves one by one; the plurality of gaps on the first induction sheet of the second rotating mechanism correspond to the plurality of test tubes, the reconstitution chamber and the first liquid-transfer valve one by one.
Further, the operating mechanism comprises a second mounting plate which is arranged opposite to the first mounting plate at a spacing; the first push-pull mechanism and the second push-pull mechanism comprise push-pull driving devices and electric fingers; the fixed end of the push-pull driving device is connected with the second mounting plate, and the movable end of the push-pull driving device is connected with the electric finger so as to drive the electric finger to reciprocate along the first direction; the electric finger of the first push-pull mechanism can clamp the first valve rod; the electric finger of the second push-pull mechanism can clamp the second valve rod.
Furthermore, the first push-pull mechanism and the second push-pull mechanism both comprise a second photoelectric switch and a second induction sheet; the second photoelectric switch is connected with the second mounting plate and is in communication connection with the push-pull driving device so as to control the start and stop of the push-pull driving device; the second sensing piece is connected with the electric finger, and the second sensing piece can move along with the electric finger so as to move to or be far away from the sensing area of the second photoelectric switch.
Further, the operating mechanism includes a first driving device; the first driving device is connected with the rack, and the movable end of the first driving device is connected with the first mounting plate so as to drive the first mounting plate to reciprocate along the first direction.
Furthermore, a guide rail and a card box driving device are arranged on the installation bearing platform; the card box is connected with the guide rail in a sliding way through a sliding plate, and the movable end of the card box driving device is connected with the sliding plate so as to enable the card box to reciprocate along a second direction; the second direction is perpendicular to the first direction.
Furthermore, the device also comprises a third photoelectric switch and a third induction sheet; the number of the third photoelectric switches is multiple, and the third photoelectric switches are distributed on the mounting bearing platform at intervals along the second direction; the third photoelectric switch is in communication connection with the card box driving device so as to control the start and stop of the card box driving device; the third induction sheet is connected with the sliding plate so as to enable the third induction sheet to reciprocate along the second direction with the sliding plate.
Further, the device also comprises an auxiliary mechanism; the auxiliary mechanism comprises a turntable, a second driving device, a sound wave vibration device and a magnetic attraction device, wherein the turntable is rotatably connected with the rack; the sound wave vibration device and the magnetic suction device are oppositely arranged on the turntable at intervals; the movable end of the second driving device is connected with the rotary disc, and the second driving device can drive the rotary disc to rotate so as to respectively drive the sound wave vibration device and the magnetic suction device to rotate to the card box.
Further, the auxiliary mechanism further comprises a third driving device and a lifting cam; the movable end of the third lifting device is connected with the lifting cam so as to drive the lifting cam to rotate; the lifting cam abuts against the rotary disc; the lifting cam rotates to drive the turntable to lift, so that the magnetic attraction device or the transduction piece of the sound wave vibration device is attached to the bottom wall of the card box.
Further, a heating element is arranged on the sound wave vibration device; the heating member can be attached to the bottom wall of the cartridge to heat the cartridge.
The invention also provides a PCR instrument comprising the sample processing device.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a sample processing device, which comprises a card box, a first liquid transferring valve and an operating mechanism; the card box forms a plurality of reagent chambers, wherein one reagent chamber is used as a sample processing chamber, one reagent chamber is used as a waste liquid chamber, and the other reagent chambers are used as processing liquid chambers which are used for storing various reagents for sample processing; the first pipetting valve is movably connected with the card box, and the operating mechanism can be connected with the first pipetting valve so as to drive the first pipetting valve to move, so that the first pipetting valve is respectively communicated with the plurality of reagent chambers, and the first pipetting valve and the communicated reagent chambers are subjected to reagent transfer; thereby make first pipetting valve under operating device's drive, transfer the reagent in the treatment solution cavity to the sample treatment cavity in proper order to transfer the waste liquid that produces after the reaction of every step is accomplished in the sample treatment cavity to the waste liquid cavity, and then conveniently obtain nucleic acid solution fast.
The invention also provides a PCR instrument, which comprises the sample processing device, so the PCR instrument also has the beneficial effects of the sample processing device.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of a sample processing device according to an embodiment of the present invention;
FIG. 2 is a schematic view of a first viewing angle of a cartridge of a sample processing device according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of an operating mechanism of a sample processing device according to an embodiment of the present invention;
FIG. 4 is a schematic view of a portion of the operating mechanism of the present invention from a first perspective;
FIG. 5 is a schematic view of a portion of the operating mechanism of the present invention from a second perspective;
FIG. 6 is a schematic structural diagram of an auxiliary mechanism of a sample processing device according to an embodiment of the present invention;
fig. 7 is a schematic structural diagram of a cartridge of a sample processing device according to an embodiment of the present invention from a second viewing angle.
Reference numerals:
1-card box, 11-reagent chamber, 12-first liquid transferring valve, 13-second liquid transferring valve, 14-test tube, 2-operating mechanism, 21-first rotating mechanism, 22-second rotating mechanism, 23-first push-pull mechanism, 24-second push-pull mechanism, 25-rotary driving device, 26-main gear, 27-auxiliary gear, 28-connecting shaft, 29-first induction sheet, 210-electric finger, 211-first mounting plate, 212-second mounting plate, 213-first driving device, 214-first photoelectric switch, 3-auxiliary mechanism, 31-rotary disk, 32-second driving device, 33-sound wave vibration device, 34-magnetic attraction device, 35-third driving device, 36-lifting cam, 37-heating element, 4-frame, 41-mounting table, 42-slide, 43-guide, 44-cartridge drive, a-first direction, b-second direction.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.
The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
A sample processing device and PCR instrument according to some embodiments of the present application are described below with reference to fig. 1-7.
The application provides a sample processing device, as shown in fig. 1 and fig. 2, comprising a card box 1, wherein the card box 1 forms a plurality of reagent chambers 11, one reagent chamber 11 is used as a sample processing chamber, one reagent chamber 11 is used as a waste liquid chamber, and the other reagent chambers 11 are used as processing liquid chambers, and the processing liquid chambers are used for storing various reagents for sample processing, such as lysis solution, cleaning solution, eluent and the like; the sample is placed in the sample treatment chamber, various reagents in the treatment chamber are sequentially transferred into the sample treatment chamber according to specified steps to react with the sample, and the generated waste liquid is transferred into the waste liquid chamber after the reaction in each step is finished, so that the nucleic acid solution is finally obtained. The sample processing device comprises a first liquid transferring valve 12 and an operating mechanism 2 which are movably connected with the card box 1; the operating mechanism 2 can be connected with the first pipetting valve 12 to drive the first pipetting valve 12 to move, so that the first pipetting valve 12 is respectively communicated with the plurality of reagent chambers 11, and the first pipetting valve 12 is communicated with the communicated reagent chambers 11 for reagent transfer; therefore, the first pipetting valve 12 is driven by the operating mechanism 2 to transfer the reagents in the treatment liquid chamber into the sample treatment chamber in sequence, and transfer the waste liquid generated after the reaction in each step in the sample treatment chamber into the waste liquid chamber, so as to conveniently and rapidly obtain the nucleic acid solution.
Preferably, as shown in fig. 3 to 5, the first liquid-moving valve 12 includes a first valve body and a first valve stem; the first valve body is rotationally connected with the card box 1 and can be respectively communicated with the plurality of reagent chambers 11 when rotating to different angles; the operating mechanism 2 includes a first rotating mechanism 21, and the first rotating mechanism 21 can be connected to the first valve body to drive the first valve body to rotate, so that the first pipetting valve 12 is respectively communicated with the plurality of reagent chambers 11. The first valve rod is positioned in the first valve body, the first valve rod can do piston motion in the first valve body, along with the reciprocating motion of the first valve rod in the first valve body, for example, the first valve rod moves upwards in the first valve body, a reagent in the reagent chamber 11 communicated with the first liquid transferring valve 12 can be extracted into the first liquid transferring valve 12, and then the first valve rod moves downwards in the first valve body, and the reagent in the first liquid transferring valve 12 can be pushed into the reagent chamber 11 communicated with the first liquid transferring valve; the operating mechanism 2 comprises a first push-pull mechanism 23, and the first push-pull mechanism 23 can be connected with the first valve rod to drive the first valve rod to perform piston motion (i.e. reciprocating motion) in the first valve body, so as to complete reagent transfer between the first liquid removing valve 12 and the reagent chamber 11 communicated with the first liquid removing valve.
After the nucleic acid extraction is completed, the nucleic acid solution in the sample processing chamber is reacted with the PCR reaction system in the form of freeze-dried beads, and the resulting reagent is filled into the test tube 14 (see, for example, fig. 2) for subsequent detection. In this embodiment, preferably, as shown in fig. 2, the cartridge 1 is formed with a reconstitution chamber for storing a PCR reaction system in the form of lyophilized beads, including buffer, polymerase or reverse transcriptase, etc.; the card box 1 is provided with a test tube rack, and a plurality of test tubes 14 are arranged on the test tube rack. The sample processing device comprises a second liquid transferring valve 13, and the second liquid transferring valve 13 has the same structure as the first liquid transferring valve 12 and comprises a second valve body rotatably connected with the card box 1 and a second valve rod positioned in the second valve body; when the second valve body rotates by different angles, the second pipetting valve 13 can be respectively communicated with the redissolution chamber and the plurality of test tubes 14; when the second valve body and the first valve body are shifted to predetermined angles, respectively, the first liquid-removing valve 12 and the second liquid-removing valve 13 can communicate with each other. As shown in fig. 1 to 4, the operating mechanism 2 includes a second rotating mechanism 22 and a second push-pull mechanism 24, the second rotating mechanism 22 can be connected with the second valve body to drive the second pipetting valve 13 to rotate, and the second push-pull mechanism 24 can be connected with the second valve stem to drive the second valve stem to perform piston movement in the second valve body, so as to complete reagent transfer between the second pipetting valve 13 and the communicated chambers. The second pipetting valve 13 acts similarly to the first pipetting valve 12.
In a specific operation process, after the sample finishes extracting nucleic acid in the sample processing chamber, the operating mechanism 2 firstly drives the first pipetting valve 12 to communicate with the sample processing chamber, and pumps the nucleic acid solution into the first pipetting valve 12 (specifically, the first push-pull mechanism 23 as described above is used to drive the first valve rod to move upwards in the first valve body, and pump the nucleic acid solution into the first pipetting valve 12); then the operating mechanism 2 drives the first pipetting valve 12 and the second pipetting valve 13 to rotate to the positions where the two are communicated, and transfers the nucleic acid solution in the first pipetting valve 12 into the second pipetting valve 13 (by using the first push-pull mechanism 23 as described above, the first valve rod is driven to move downwards in the first valve body, and the nucleic acid solution in the first pipetting valve 12 is transferred into the second pipetting valve 13); then the operating mechanism 2 drives the second pipetting valve 13 to rotate to a position communicated with the re-dissolving chamber, so as to push the nucleic acid solution into the re-dissolving chamber (the second valve rod is driven to move downwards in the second valve body by the second push-pull mechanism 24, so as to push the nucleic acid solution into the re-dissolving chamber); after the redissolution reaction is completed, the operating mechanism 2 drives the second pipetting valve 13 to draw the reagent in the redissolution chamber into the second pipetting valve 13 (the second push-pull mechanism 24 drives the second valve rod to move upwards in the second valve body to draw the reagent in the redissolution chamber into the second pipetting valve 13), and then the second pipetting valve 13 is sequentially communicated with the plurality of test tubes 14, and the reagent in the second pipetting valve 13 is respectively conveyed into the plurality of test tubes 14 (the second push-pull mechanism 24 drives the second valve rod to move downwards in the second valve body to respectively convey the reagent in the second pipetting valve 13 into the plurality of test tubes 14), so that the filling of the test tubes 14 is completed, and the subsequent detection is performed.
In this embodiment, as shown in fig. 1 to 5, the sample processing apparatus includes a frame 4, a cartridge 1 is horizontally placed on a mounting platform 41 of the frame 4, an axial direction of a rotational connection of a first liquid-moving valve 12 and a second liquid-moving valve 13 with the cartridge 1 extends in a first direction a, i.e., a vertical direction, and a first valve stem and a second valve stem are capable of reciprocating in the vertical direction.
As shown in fig. 3 to 5, the operating mechanism 2 includes a first mounting plate 211, the first mounting plate 211 is disposed above the cartridge 1 in the horizontal direction, and the first mounting plate 211 is connected to the mounting platform 41; the first rotating mechanism 21 and the second rotating mechanism 22 are both mounted on the first mounting plate 211, and the first rotating mechanism 21 is the same as the second rotating mechanism 22, so for clarity, the corresponding structures of the first rotating mechanism 21 and the second rotating mechanism 22 are described here by taking the first rotating mechanism 21 as an example.
In this embodiment, preferably, as shown in fig. 4 and 5, the first rotation mechanism 21 includes a connecting shaft 28, a rotation driving device 25, a main gear 26, and a sub-gear 27; the fixed end of the rotation driving device 25 is mounted on the first mounting plate 211, and the movable end of the rotation driving device 25 is connected to the main gear 26 to drive the main gear 26 to rotate. The connecting shaft 28 is arranged on the first mounting plate 211 along the vertical direction, and the connecting shaft 28 is rotatably connected with the first mounting plate 211; the pinion 27 is sleeved on the connecting shaft 28, and the pinion 27 is engaged with the main gear 26, so that when the rotation driving device 25 drives the main gear 26 to rotate, the pinion 27 and the connecting shaft 28 can rotate along with the main gear 26. The connecting shaft 28 can be connected with the first valve body, so as to drive the first valve body to rotate along with the first valve body; the connecting shaft 28, the first valve body, and the pinion 27 are coaxially disposed. Therefore, the main gear 26 is driven by the rotation driving device 25 to rotate by different angles, so that the first valve body can rotate by different angles, and the first pipetting valve 12 is communicated with the plurality of reagent chambers 11 and the second pipetting valve 13 respectively; similarly, the second pipetting valve 13 can be rotated by different angles by the second rotation mechanism 22, and the second pipetting valve 13 is made to communicate with the first pipetting valve 12, the reconstitution chamber, and the plurality of cuvettes 14, respectively.
In this embodiment, preferably, the connection between the connecting shaft 28 of the first rotation mechanism 21 and the first valve body is a detachable connection, and likewise, the connection between the connecting shaft 28 of the second rotation mechanism 22 and the second valve body is a detachable connection; taking the connection between the connection shaft 28 of the first rotation mechanism 21 and the first valve body as an example, preferably, a connection part is formed on the outer wall of the first valve body, and the connection part is hexagonal; a connecting hole is formed in one end, facing the first valve body, of the connecting shaft 28, and the connecting hole is hexagonal and is matched with the connecting part on the first valve body, so that the connecting hole can be sleeved on the connecting part; so that when the connecting shaft 28 is rotated, the first valve body can be rotated therewith. Preferably, the connecting shaft 28 is hollow, and when the end of the connecting shaft 28 facing the first valve body is sleeved on the first valve body, the first valve rod can extend out from the end of the connecting shaft 28 far away from the first valve body.
In this embodiment, preferably, as shown in fig. 4 and 5, each of the first and second rotating mechanisms 21 and 22 includes a first photoelectric switch 214 and a first sensing piece 29 to control the rotation angles of the first and second pipetting valves 12 and 13; the first and second rotating mechanisms 21 have the same structure, and the description will be given by taking the first rotating mechanism as an example. Preferably, the first sensing piece 29 is circular, the first sensing piece 29 is connected to the main gear 26 and is coaxially disposed, and an edge portion of the first sensing piece 29 extends out of the main gear 26. The first photoelectric switch 214 is fixedly arranged on the first mounting plate 211, and the edge part of the first sensing sheet 29 can extend into the sensing area of the first photoelectric switch 214; a plurality of gaps are formed on the edge part of the first sensing piece 29, wherein one gap corresponds to the second pipetting valve 13, and the other gaps correspond to the plurality of reagent chambers 11 one by one; namely, when the notch corresponding to the second pipetting valve 13 rotates to the position of the first photoelectric switch 214, the first pipetting valve 12 can be communicated with the second pipetting valve 13; when the rest of the gaps rotate to the position of the first photoelectric switch 214, the first pipetting valve 12 can be communicated with the reagent chamber 11 corresponding to the gap; the first photoelectric switch 214 is in communication connection with the rotation driving device 25, when the main gear 26 rotates a predetermined angle to connect the first pipetting valve 12 with one reagent chamber 11, the corresponding notch of the reagent chamber 11 rotates to the first photoelectric switch 214, and the first photoelectric switch 214 controls the rotation driving device 25 to stop working, so that the first pipetting valve 12 stays at the position where the first pipetting valve 12 is connected with the reagent chamber 11; therefore, the rotation angle of the first pipetting valve 12 is precisely controlled by the first photoelectric switch 214 and the first sensing piece 29, so that the first pipetting valve 12 can be precisely rotated to a position where the corresponding reagent chamber 11 and the second pipetting valve 13 are communicated. The second rotating mechanism 22 has the same structure as the first rotating mechanism 21, and therefore, the detailed description thereof is omitted, and the first photoelectric switch 214 and the first sensing piece 29 of the second rotating mechanism 22 can accurately control the rotation angle of the second pipetting valve 13, so that the second pipetting valve 13 can be respectively rotated to the positions communicated with the first pipetting valve 12, the reconstitution chamber and the plurality of test tubes 14.
In one embodiment of the present application, preferably, as shown in fig. 1 and 3, the operating mechanism 2 further includes a second mounting plate 212, the second mounting plate 212 is located above the first mounting plate 211 and is disposed opposite to the first mounting plate 211, and the first push-pull mechanism 23 and the second push-pull mechanism 24 are mounted on the second mounting plate 212. The first push-pull mechanism 23 and the second push-pull mechanism 24 each include a push-pull driving device and a power finger 210, which are identical in structure, and for the sake of clarity, the first push-pull mechanism 23 will be described as an example. Preferably, the fixed end of the push-pull driving device of the first push-pull mechanism 23 is mounted on the second mounting plate 212, and the movable end of the push-pull driving device is connected with the power finger to drive the power finger 210 to reciprocate along the vertical direction. The first valve rod of the first pipetting valve 12 extends into the space between the first mounting plate 211 and the second mounting plate 212 through the connecting shaft 28; the push-pull driving device drives the electric finger 210 to descend to the position of the first valve rod, the electric finger 210 can clamp the first valve rod, then the push-pull driving device drives the electric finger 210 to reciprocate along the first direction a, the first valve rod is driven to do piston motion in the first valve body, and therefore reagent transfer between the first liquid transferring valve 12 and the communicated reagent chamber 11 or the second liquid transferring valve 13 is completed. Preferably, the push-pull driving device comprises a motor and a screw rod, the motor is fixedly mounted on the second mounting plate 212, the screw rod extends along the vertical direction, one end of the screw rod is connected with the output end of the motor through a coupler, and the other end of the screw rod is rotatably connected with the first mounting plate 211; the electric finger 210 top is provided with the deflector, offers the screw hole with screw rod looks adaptation on the deflector to make the deflector can with screw rod looks spiro union, thereby when motor drive screw rod around clockwise or anticlockwise rotation, the deflector can drive electric finger 210 along vertical direction reciprocating motion. Preferably, still be provided with the guide bar that extends along vertical direction between first mounting panel 211 and the second mounting panel 212, seted up the guiding hole on the deflector, the guide bar passes through the guiding hole cover and locates on the guide bar to guarantee that deflector and electronic finger 210 can follow vertical direction reciprocating motion all the time.
In this embodiment, the first push-pull mechanism 23 and the second push-pull mechanism 24 preferably each further include a second photoelectric switch and a second sensing piece, which will be described below as the first push-pull mechanism 23; the second photoelectric switch is arranged on the second mounting plate 212, the second sensing piece is arranged on the guide plate, and the second sensing piece can reciprocate along with the electric finger 210 and the guide plate in the vertical direction; the second photoelectric switch is in communication connection with the push-pull driving device so as to control the start and stop of the push-pull driving device. In the initial position, the first valve rod is located at the lowest end in the first valve body, and the first valve rod needs to be pulled upwards to draw the reagent into the first liquid transfer valve 12; the push-pull driving device drives the electric finger 210 to move downwards to the first valve rod, the second sensing piece moves downwards along with the electric finger 210 and moves to a sensing area of a second photoelectric switch, the second photoelectric switch controls the push-pull driving device to stop moving, the electric finger 210 stays at the position of the first valve rod, and then the electric finger 210 clamps the first valve rod; the push-pull actuator then continues to operate to move the power finger 210 upward to pull the first valve stem upward.
In one embodiment of the present application, preferably, as shown in fig. 3, the sample processing device further includes a first driving device 213, the first driving device 213 is fixedly mounted on the frame 4, and a movable end of the first driving device 213 is connected to the first mounting plate 211 to drive the first mounting plate 211 and the operating mechanism 2 on the first mounting plate 211 to reciprocate along the first direction a, i.e., the vertical direction. In a specific operation, the first driving device 213 first drives the first mounting plate 211 to move up to the standby position, and places the cartridge 1 at the sample processing position below the first mounting plate 211, and then the first driving device 213 drives the first mounting plate 211 to move down to the operating position, so that the lower end of the connecting shaft 28 of the first rotating mechanism 21 is connected to the first valve body, the lower end of the connecting shaft 28 of the second rotating mechanism 22 is connected to the second valve body, and the first valve rod and the second valve rod respectively penetrate through the corresponding connecting shafts 28 and extend into the space between the first mounting plate 211 and the second mounting plate 212. Preferably, the first driving device 213 is mounted on the frame 4 through a connecting plate, the connecting plate is disposed opposite to the mounting platform 41 of the frame 4, and a connecting rod extending along the vertical direction is disposed between the connecting plate and the mounting platform 41; connecting through holes are formed in the first mounting plate 211, so that the mounting plate is sleeved on the connecting rod, and the effect of guiding the lifting of the first mounting plate 211 can be achieved through the connecting rod. First drive arrangement 213 includes motor and connecting screw, motor fixed mounting is on the connecting plate, connecting screw extends along vertical direction, and connecting screw's one end is connected with the output of motor through the shaft coupling, connecting screw's the other end rotates with installation cushion cap 41 to be connected, set up the threaded connection hole with connecting screw looks adaptation on the first mounting panel 211, so that first mounting panel 211 and connecting screw looks spiro union, when motor drive connecting screw clockwise or anticlockwise rotates, vertical direction reciprocating motion can be followed to first mounting panel 211, thereby drive first rotary mechanism 21 that is located first mounting panel 211 top, second rotary mechanism 22, first push-and-pull mechanism 23 and second push-and-pull mechanism 24 are along vertical square reciprocating motion.
Preferably, an induction sheet is arranged on the first mounting plate 211, two photoelectric induction switches are arranged between the connecting plate and the mounting platform 41 at intervals along the vertical direction, and both the two photoelectric switches can control the start and stop of the first driving device 213. When the first mounting plate 211 and the sensing piece are moved to the position of the photoelectric switch located above, the first mounting plate 211 is located at the standby station, and the cartridge 1 may be placed at the sample processing station below the first mounting plate 211; when the first mounting plate 211 and the sensing piece move to the position of the photoelectric switch located below, the first mounting plate 211 is located at the operation station, and the operation mechanism 2 can be connected with the first pipetting valve 12 and the second pipetting valve 13 to drive the first pipetting valve 12 and the second pipetting valve 13 to rotate and perform reagent transferring operation.
In one embodiment of the present application, the cartridge 1 is preferably slidably connected to a mounting platform 41, as shown in fig. 2 and 7; the mounting bearing platform 41 is provided with a guide rail 43 and a card box driving device 44, the guide rail 43 extends along a second direction b which is perpendicular to the first direction a, the middle part of the guide rail 43 is positioned right below the first mounting plate 211, and two ends of the guide rail 43 extend out of the first mounting plate 211; the cartridge 1 is slidably coupled to the guide rail 43 by the slide plate 42, and the movable end of the cartridge driving device 44 is coupled to the slide plate 42 to reciprocate the cartridge 1 in the second direction b. The card box 1 has three stations on the guide rail 43, firstly, an initial station is arranged at one end of the guide rail 43, the card box 1 is placed on the guide rail 43 by an operator, secondly, a second station is arranged below the first mounting plate 211, namely, a sample processing station, the card box 1 is driven by the card box driving device 44 to move to the sample processing station, and the first liquid transferring valve 12 and the second liquid transferring valve 13 are driven by the operating mechanism 2 to move to complete the extraction of nucleic acid and the filling of the test tube 14; and finally, after the filling of the test tube 14 is completed at the third station positioned at the other end of the guide rail 43, the card box driving device 44 drives the card box 1 to move to the third station so as to cooperate with subsequent equipment of the PCR instrument to complete subsequent detection.
In this embodiment, preferably, in order to match the movement of the cartridge 1 between the three stations on the guide rail 43, three third photoelectric switches are disposed on the mounting platform 41, and a third sensing piece is disposed on the sliding plate 42; when the card box 1 and the third induction sheet move to three stations in sequence, the third induction sheet also moves to the positions of three third photoelectric switches respectively; the three third photoelectric switches are in communication connection with the card box driving device 44 to control the start and stop of the card box driving device; thereby precisely controlling the movement of the slide plate 42 and the cartridge 1 to precisely move the cartridge 1 at the first station to the second station and the third station.
In one embodiment of the present application, preferably, as shown in fig. 1 and 6, the sample processing device includes an auxiliary mechanism 3; the auxiliary mechanism 3 comprises an acoustic wave vibration device 33, a magnetic suction device 34, a second driving device 32 and a turntable 31, the turntable 31 is positioned below the mounting bearing platform 41 and is rotatably connected with the rack 4, and the movable end of the second driving device 32 is connected with the turntable 31 so as to drive the turntable 31 to rotate; the acoustic wave vibration device 33 and the magnetic attraction device 34 are fixedly installed on the turntable 31, and the acoustic wave vibration device 33 and the magnetic attraction device 34 can be sequentially transferred to the position right below the sample processing chamber of the cartridge 1 through the turntable 31. The auxiliary mechanism 3 further includes a third driving device 35 and a lifting cam 36, a movable end of the third driving device 35 is connected to the lifting cam 36 to drive the lifting cam 36 to rotate, the lifting cam 36 abuts against a lower end of the rotary plate 31, and the rotary plate 31 can be lifted and lowered along a vertical direction along with the rotation of the cam. In the sample processing process, when a sample reacts with various reagents in the sample processing chamber, firstly, the acoustic wave vibration device 33 rotates to the position below the sample processing chamber, and then the third driving device 35 drives the acoustic wave vibration device 33 to ascend, so that the transduction piece of the acoustic wave vibration device 33 abuts against the bottom wall of the card box 1 below the sample processing chamber; therefore, the vibration generated by the acoustic vibration device 33 is directly transmitted into the sample processing chamber, which can effectively help the pathogen cells in the sample processing chamber to be broken and uniformly mixed with various different reaction reagents, especially with magnetic beads. When reagent to in the sample processing chamber shifts, will make the magnetic bead not transferred away, consequently when the process of shifting reagent, need inhale device 34 with magnetism and shift to the department of sample processing chamber to make magnetism inhale device 34 and laminate mutually with the lateral wall of sample processing chamber through third drive arrangement 35, attract the magnetic bead to the one side of keeping away from the liquid outlet of sample processing chamber, thereby prevent that the magnetic bead from flowing out along with the reagent. Preferably, the mounting platform 41 is provided with a through hole, and the magnetic attraction device 34 and the sound wave vibration device 33 can project through the through hole and extend into the cartridge 1 above the mounting platform 41.
In this embodiment, preferably, as shown in FIG. 6, since some process characteristics require a certain temperature during the sample processing, a heating element 37 is provided on the sonic vibrator 33, and when the transducer sheet of the sonic vibrator 33 is engaged with the cartridge 1, the heating element 37 is also engaged with the cartridge 1 to provide heat for the reaction in the sample processing chamber.
In this embodiment, preferably, the turntable 31 is provided with a sensing piece, and the frame 4 is provided with a photoelectric switch communicatively connected to the second driving device 32, so as to control the rotation angle of the turntable 31, so that the magnetic attraction device 34 and the sound wave vibration device 33 can rotate to the correct position. The lifting cam 36 is also provided with a reaction piece, and correspondingly the frame 4 is provided with a photoelectric switch, and the photoelectric switch can sense the position of the lifting cam 36 so as to control the lifting of the turntable 31.
The application also provides a PCR instrument comprising the sample processing device of any of the above embodiments.
In this embodiment, the PCR instrument includes the sample processing device, so the PCR instrument has all the advantages of the sample processing device, and is not described in detail herein.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (14)
1. A sample processing device comprising a cartridge, a first pipetting valve and an operating mechanism;
the cartridge body forms a plurality of reagent chambers, the first pipetting valve being movably connected to the cartridge;
the operating mechanism can be connected with the first pipetting valve, and the operating mechanism can drive the first pipetting valve to move so as to enable the first pipetting valve to be respectively communicated with the plurality of reagent chambers and enable the first pipetting valve to be communicated with the reagent chambers to carry out reagent transfer.
2. The sample processing device of claim 1, wherein the first pipetting valve comprises a first valve body and a first valve stem disposed within the first valve body that extends in a first direction;
the first valve body is rotatably connected with the card box;
the operating mechanism comprises a first rotating mechanism and a first push-pull mechanism;
the first rotating mechanism can be connected with the first valve body so as to drive the first valve body to rotate; the first push-pull mechanism can be connected with the first valve rod to drive the first valve rod to reciprocate along the first direction, so that liquid in the first liquid removing valve can be discharged to a corresponding reagent chamber or liquid in the corresponding reagent chamber can be extracted into the first liquid removing valve.
3. The sample processing device of claim 2, further comprising a second pipetting valve and a tube rack;
the card box is provided with a redissolution chamber, the test tube rack is arranged on the card box, and a plurality of test tubes are arranged on the test tube rack;
the second pipetting valve comprises a second valve body and a second valve rod which is arranged in the second valve body and extends along the first direction;
the second valve body is rotatably connected with the card box and can rotate by different angles so as to enable the second pipetting valve to be respectively communicated with the first pipetting valve, the redissolution chamber and the plurality of test tubes;
the operating mechanism comprises a second rotating mechanism and a second push-pull mechanism;
the second rotating mechanism can be connected with the second valve body so as to drive the second valve body to rotate; the second push-pull mechanism can be connected with the second valve rod to drive the second valve rod to reciprocate along the first direction, so that liquid in the second liquid transferring valve can be discharged or extracted.
4. The sample processing device of claim 3, comprising a rack;
the card box is placed on an installation bearing platform of the frame; the operating mechanism comprises a first mounting plate, the first mounting plate and the mounting bearing platform are oppositely arranged along the first direction at intervals, and the clamping box is positioned between the mounting bearing platform and the first mounting plate;
the first rotating mechanism and the second rotating mechanism are positioned on the first mounting plate, and both the first rotating mechanism and the second rotating mechanism comprise a connecting shaft, a rotation driving device, a main gear and a pinion;
the connecting shaft is arranged along the first direction and is rotatably connected with the first mounting plate; the fixed end of the rotary driving device is connected with the first mounting plate, and the movable end of the rotary driving device is connected with the main gear so as to drive the main gear to rotate; the auxiliary gear is sleeved on the connecting shaft and meshed with the main gear;
the connecting shaft of the first rotating mechanism can be connected with the first valve body, and the connecting shaft of the second rotating mechanism can be connected with the second valve body.
5. The sample processing device of claim 4, wherein the first and second rotation mechanisms each comprise a first photoelectric switch and a first sensing tab;
the first photoelectric switch is arranged on the first mounting plate and is in communication connection with the rotary driving device so as to control the start and stop of the rotary driving device; the first sensing piece is connected with the main gear and can rotate along with the main gear;
a plurality of openings are formed in the edge part of the first induction sheet and are distributed at intervals in the circumference;
the plurality of openings on the first sensing sheet of the first rotating mechanism correspond to the plurality of reagent chambers and the second pipetting valves one by one; the plurality of gaps on the first induction sheet of the second rotating mechanism correspond to the plurality of test tubes, the reconstitution chamber and the first liquid-transfer valve one by one.
6. The sample processing device of claim 4, wherein the manipulation mechanism comprises a second mounting plate spaced opposite the first mounting plate;
the first push-pull mechanism and the second push-pull mechanism comprise push-pull driving devices and electric fingers;
the fixed end of the push-pull driving device is connected with the second mounting plate, and the movable end of the push-pull driving device is connected with the electric finger so as to drive the electric finger to reciprocate along the first direction;
the electric finger of the first push-pull mechanism can clamp the first valve rod; and an electric finger of the second push-pull mechanism can clamp the second valve rod.
7. The sample processing device of claim 6, wherein the first push-pull mechanism and the second push-pull mechanism each comprise a second opto-electronic switch and a second sensing strip;
the second photoelectric switch is connected with the second mounting plate and is in communication connection with the push-pull driving device so as to control the start and stop of the push-pull driving device;
the second sensing piece is connected with the electric finger, and the second sensing piece can move along with the electric finger so as to move to or far away from a sensing area of the second photoelectric switch.
8. The sample processing device of claim 4, further comprising a first drive device;
the first driving device is connected with the rack, and the movable end of the first driving device is connected with the first mounting plate so as to drive the first mounting plate to reciprocate along the first direction.
9. The sample processing device of claim 4, wherein the mounting platform is provided with a guide rail and a cartridge drive device;
the card box is connected with the guide rail in a sliding way through a sliding plate, and the movable end of the card box driving device is connected with the sliding plate so as to enable the card box to reciprocate along a second direction;
the second direction is perpendicular to the first direction.
10. The sample processing device of claim 9, further comprising a third photoelectric switch and a third sensor strip;
the number of the third photoelectric switches is multiple, and the third photoelectric switches are distributed on the mounting bearing platform at intervals along the second direction; the third photoelectric switch is in communication connection with the card box driving device so as to control the start and stop of the card box driving device;
the third induction sheet is connected with the sliding plate so as to enable the third induction sheet to reciprocate along the second direction with the sliding plate.
11. The sample processing device of claim 4, further comprising an assist mechanism;
the auxiliary mechanism comprises a turntable, a second driving device, a sound wave vibration device and a magnetic attraction device, wherein the turntable is rotatably connected with the rack;
the sound wave vibration device and the magnetic suction device are oppositely arranged on the turntable at intervals;
the movable end of the second driving device is connected with the rotary disc, and the second driving device can drive the rotary disc to rotate so as to respectively drive the sound wave vibration device and the magnetic suction device to rotate to the card box.
12. The sample processing device of claim 11, wherein the assist mechanism further comprises a third drive device and a lift cam;
the movable end of the third driving device is connected with the lifting cam so as to drive the lifting cam to rotate;
the lifting cam abuts against the rotary disc; the lifting cam rotates to drive the rotary disc to lift along the first direction, so that the magnetic suction device or the transduction piece of the sound wave vibration device is attached to the bottom wall of the clamping box.
13. The sample processing device of claim 12, wherein the sonic vibration device is provided with a heating element;
the heating member can be attached to the bottom wall of the cartridge to heat the cartridge.
14. A PCR instrument comprising the sample processing device of any one of claims 1 to 13.
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| CN202010299980.5A CN113528287B (en) | 2020-04-16 | 2020-04-16 | Sample processing device and PCR instrument |
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| CN202010299980.5A CN113528287B (en) | 2020-04-16 | 2020-04-16 | Sample processing device and PCR instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980776A (en) * | 2021-12-06 | 2022-01-28 | 广东润鹏生物技术有限公司 | Cartridge, sample processing device, and molecular diagnostic system |
| CN114164091A (en) * | 2021-12-07 | 2022-03-11 | 广东润鹏生物技术有限公司 | Molecular diagnostic sample processing system and control method |
| WO2023206094A1 (en) * | 2022-04-26 | 2023-11-02 | 广州国家实验室 | Device for adapting to use of cartridge |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2740589Y (en) * | 2004-06-11 | 2005-11-16 | 杭州华大基因研发中心 | Liquid transferring device and component of pressure variable cavity therewith |
| AU2013205268A1 (en) * | 2007-07-13 | 2013-05-02 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN103540517A (en) * | 2010-07-23 | 2014-01-29 | 贝克曼考尔特公司 | System and method including analytical units |
| CN105974145A (en) * | 2016-05-17 | 2016-09-28 | 山东栋梁科技设备有限公司 | Full-automatic Newcastle disease detector |
| CN206814737U (en) * | 2017-05-24 | 2017-12-29 | 苏州天隆生物科技有限公司 | A kind of nucleic acid extraction purification devices |
| CN108226550A (en) * | 2018-01-22 | 2018-06-29 | 上海默礼生物医药科技有限公司 | A kind of chemical luminescence reagent kit and its detection method of miniature more bin controls |
| CN108490205A (en) * | 2018-04-10 | 2018-09-04 | 深圳市博辰智控有限公司 | A kind of microwell plate sample adding system and method |
| CN111004797A (en) * | 2019-12-06 | 2020-04-14 | 深圳市宝安区沙井人民医院 | Nucleic acid extraction device and nucleic acid extraction method |
-
2020
- 2020-04-16 CN CN202010299980.5A patent/CN113528287B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2740589Y (en) * | 2004-06-11 | 2005-11-16 | 杭州华大基因研发中心 | Liquid transferring device and component of pressure variable cavity therewith |
| AU2013205268A1 (en) * | 2007-07-13 | 2013-05-02 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN103540517A (en) * | 2010-07-23 | 2014-01-29 | 贝克曼考尔特公司 | System and method including analytical units |
| CN105974145A (en) * | 2016-05-17 | 2016-09-28 | 山东栋梁科技设备有限公司 | Full-automatic Newcastle disease detector |
| CN206814737U (en) * | 2017-05-24 | 2017-12-29 | 苏州天隆生物科技有限公司 | A kind of nucleic acid extraction purification devices |
| CN108226550A (en) * | 2018-01-22 | 2018-06-29 | 上海默礼生物医药科技有限公司 | A kind of chemical luminescence reagent kit and its detection method of miniature more bin controls |
| CN108490205A (en) * | 2018-04-10 | 2018-09-04 | 深圳市博辰智控有限公司 | A kind of microwell plate sample adding system and method |
| CN111004797A (en) * | 2019-12-06 | 2020-04-14 | 深圳市宝安区沙井人民医院 | Nucleic acid extraction device and nucleic acid extraction method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113980776A (en) * | 2021-12-06 | 2022-01-28 | 广东润鹏生物技术有限公司 | Cartridge, sample processing device, and molecular diagnostic system |
| CN114164091A (en) * | 2021-12-07 | 2022-03-11 | 广东润鹏生物技术有限公司 | Molecular diagnostic sample processing system and control method |
| WO2023103738A1 (en) * | 2021-12-07 | 2023-06-15 | 广东润鹏生物技术有限公司 | Molecular diagnostic sample processing system and control method |
| WO2023206094A1 (en) * | 2022-04-26 | 2023-11-02 | 广州国家实验室 | Device for adapting to use of cartridge |
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| CN113528287B (en) | 2022-12-23 |
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