CN108226550A - A kind of chemical luminescence reagent kit and its detection method of miniature more bin controls - Google Patents
A kind of chemical luminescence reagent kit and its detection method of miniature more bin controls Download PDFInfo
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- CN108226550A CN108226550A CN201810061122.XA CN201810061122A CN108226550A CN 108226550 A CN108226550 A CN 108226550A CN 201810061122 A CN201810061122 A CN 201810061122A CN 108226550 A CN108226550 A CN 108226550A
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- Prior art keywords
- bin
- miniature
- chemical luminescence
- reagent kit
- luminescence reagent
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Chemical luminescence reagent kit and its detection method, the kit the invention discloses a kind of miniature more bin controls include：Reagent box body, it is made of box main body, soft rubber cushion and welding plate, it is set on box main body there are six the shaft that liquid cabin, four reaction warehouses, a gas storehouse, a shaft storehouse and a dryer bin are rotatably disposed in syringe-like structure in shaft storehouse, is made of cavity, push rod and rubber plug and forms bin；Renovating at the top of reagent box body and side cover are set to, at the top of sealant box body；And the bottom cover of reagent box body bottom is set to, for sealant box body bottom.Chemical luminescence reagent kit provided by the invention, reaction process is allocated mixing and reaction by project regulated procedure to each component, entire test process is completed inside kit, pollution-free, can realize quick, the highly sensitive and quantitative detection to multiple projects simultaneously.
Description
Technical field
The present invention relates to technical field of medical detection more particularly to a kind of chemical luminescence reagent kits of miniature more bin controls
And its application method.
Background technology
In recent years, China's external diagnosis reagent (IVD) is grown rapidly.Immune class reagent almost occupies half of the country.Wherein,
Chemiluminescence and immunochromatography are in occupation of the absolute market share.
At present, class external diagnosis reagent is immunized mainly to both direction to develop：
1) high-throughput, automation and the chemiluminescence route of high-quality：Its main representative for Roche, Abbott Laboratories, Siemens and
The imports such as Beckman manufacturer.Domestic has Shenzhen NPD projects, mikey, micro- high, Antu, long brilliance doctor and China of section etc..This kind equipment
For high-throughput, dynamicization and quantify.
2) miniature and portability route：This POCT namely usually said (fluorescence immune chromatography) market, inspires confidence in and base with ten thousand
Egg biology is representative, and this kind of test strips, advantage is exactly portable, quick；The equally apparent accuracy of shortcoming is limited.
With the development of technology, the demand in market slowly develops towards accurate, quantitative, miniature and portability direction.Thus
Very small chemical luminescence reagent and instrument are expedited the emergence of.This also complies with the needs of China's IVD market developments.Wherein than more typical producer
There are the micro- POCT chemiluminescence detection kits of Shenzhen Hua Maixing.But its shortcoming, can't be complete it is also obvious that single but item detection
The full immunochromatography strip substituted in the market.
Invention content
The present invention proposes anti-inside a kind of automation of miniature more bin controls to solve the above problem of the prior art
Answer chemical luminescence reagent kit and its detection method.
Large-sized luminous instrument is detected the step needed for a project by the microchamber control chemiluminescence of the present invention, design concept
It is miniaturized, by needed for accurate control of the liquid between multiple bins and the detection of transfer replication large size automatic luminous instrument
Step.1~4 project can be detected simultaneously, and application prospect is very extensive.
To achieve the above object, the present invention uses following technical scheme：
The present invention provides a kind of chemical luminescence reagent kit of miniature more bin controls, including：
Reagent box body is made of box main body, soft rubber cushion and welding plate, is set on the box main body there are six liquid cabin/tetra-
Reaction warehouse, a gas storehouse, a shaft storehouse and a dryer bin；Each liquid cabin and the reaction warehouse bottom are all provided with
There is the first aperture；Four the first apertures of the reaction warehouse bottom are connected to described turn by groove of the bottom four with boss
Around axis storehouse, and annular spread is formed with six the first apertures of six liquid cabins, bottom；Four reaction warehouse upper ends
Groove is further respectively had, venthole is offered between the dryer bin and gas storehouse, the reaction warehouse is made to be communicated with gas storehouse；
The shaft of syringe-like structure in the shaft storehouse is rotatably disposed in, by cavity, push rod and rubber plug-form
And bin is formed, the cavity is additionally provided with trepanning, and the trepanning can be connected with the bin；The push rod upper end is additionally provided with second
Hook, the rubber plug are fixed to the push rod bottom；
Renovating at the top of the reagent box body and side cover are set to, for sealing at the top of described dose of box body；And
The bottom cover of the reagent box body bottom is set to, for sealing described dose of box body bottom.
Further, on the chemical luminescence reagent kit of miniature more bin controls, the dryer bin both sides are also
Equipped with fixture block, positioning use when the fixture block is as welding light-transmitting plate.
Further, on the chemical luminescence reagent kit of miniature more bin controls, the box main body bottom is also set
It is useful for fixing the card slot of the bottom cover.
Further, on the chemical luminescence reagent kit of described miniature more bins controls, set in the welding plate there are four
Second orifice corresponding with the groove, the upper side of the welding plate are equipped with the soft rubber cushion, when the welding plate by welding or
After other modes are fixed to the box main body bottom, the soft rubber cushion engages to form four with the groove clamping with boss
Fluid passage connects the aperture with four through-holes respectively with the reaction warehouse bottom.
It is four described second small it is further preferred that on the chemical luminescence reagent kit of described miniature more bins controls
The Kong Yuliu liquid cabins, the first through hole of bottom form one group in conplane annular spread hole.
Further, on the chemical luminescence reagent kit of miniature more bin controls, the cavity bottom is additionally provided with
Second card slot, for the chemical luminescence reagent kit to be fixed to the drive shaft of detection device by second card slot.
Further, on the chemical luminescence reagent kit of described miniature more bins controls, it is described renovate by fixed cover and
Removable cover forms, and the fixed cover is equipped with the first circular hole, adding mouth and six with leachy stomata boss；The removable cover
The second circular hole corresponding with six stomata boss and face of weld are equipped with, sealed membrane is laid on the face of weld.
It is further preferred that on the chemical luminescence reagent kit of miniature more bin controls, the bottom cover is equipped with
Round boss is arranged in the first hook and the wedge shape positioned at its bottom that the round boss surrounding is agreed with the card slot
Slot.
It is further preferred that on the chemical luminescence reagent kit of miniature more bin controls, it is described including being set to
It states the light-transmitting plate at the top of dryer bin and four reaction warehouses, sets that there are four correspond to the reaction warehouse on the light-transmitting plate
Round light transmission piece, both sides, which are equipped with, to be buckled in the bayonet, the bayonet on the fixture block.
The second aspect of the invention is to provide a kind of detection of the chemical luminescence reagent kit of miniature more bin controls
Method specifically comprises the following steps：
(1) side cover E is removed；
(2) removable cover renovated is opened, sealed membrane is made to be detached with leachy stomata boss 23；
(3) at sample application mouth 22,50~250 microlitres of serum or blood plasma are added in into liquid cabin 3, and cover removable cover
C2；
(4) rotating shaft B draws 50~200 microlitres of serum or blood plasma in liquid cabin 3；
(5) continue rotating shaft B to mix the serum of absorption and blood plasma with the magnetic bead in liquid cabin 6；
(6) continue rotating shaft B the magnetic bead serum in liquid cabin 6 or plasma pool are injected into the reaction warehouse 7
Dissolving ligand particle is reacted；
(7) after reaction, the reaction liquid in reaction warehouse 7 is transferred to waste liquid storehouse；
(8) cleaning solution cleans 1~3 time；
(9) filling luminescent solution detection.
The present invention compared with prior art, is had the following technical effect that using above-mentioned technical proposal：
The chemical luminescence reagent kit of miniature more bin controls provided by the invention, using Beads enrichment technology or oligonucleotides
Isolation technics, antibody particle freeze drying technology and miniature instrument are marked, the required component of detection process is placed in specific storehouse；
Reaction process is allocated each component mixing and reaction by project regulated procedure, and entire test process is complete inside kit
Into, it is pollution-free, quick, the highly sensitive and quantitative detection to multiple projects can be realized simultaneously.
Description of the drawings
Fig. 1 is a kind of overall structure diagram of the chemical luminescence reagent kit of miniature more bin controls of the present invention；
Fig. 2 is a kind of integrally-built assembling schematic diagram of the chemical luminescence reagent kit of miniature more bin controls of the present invention；
Fig. 3,4,5 show for the structure of reagent box main body in a kind of chemical luminescence reagent kit of miniature more bin controls of the present invention
It is intended to；
Fig. 6,7 structure diagram for shaft in a kind of chemical luminescence reagent kit of miniature more bin controls of the present invention；
Fig. 8 is the structure diagram of top flip in a kind of chemical luminescence reagent kit of miniature more bin controls of the present invention；
Fig. 9 is the structure diagram of bottom cover in a kind of chemical luminescence reagent kit of miniature more bin controls of the present invention；
Figure 10 is the structure diagram of light-transmitting plate in a kind of chemical luminescence reagent kit of miniature more bin controls of the present invention；
Figure 11 is the section view of kit internal structure in a kind of chemical luminescence reagent kit of miniature more bin controls of the present invention
Figure；
Wherein, each reference numeral is：
A- reagent box bodys, A1- box main bodies, A2- soft rubber cushions, A3- welding plates；B- shafts, B1- cavitys, B2- push rods, B3- glue
Plug；C- is renovated, C1- fixed covers, C2- removable covers, D- bottom covers, E- side covers, F- light-transmitting plates, G- sealed membranes, 1-6- liquid cabins, and 7- is anti-
Ying Cang, 8- gas storehouse, 9- shafts storehouse, 10- dryer bins, 11- ventholes, 12- fixture blocks, 13- grooves, the first card slots of 14-, 15- ditches
Slot, 16- second orifices, 17- bins, the first hooks of 18-, 19- trepannings, the second card slots of 20-, the first circular holes of 21-, 22- adding mouths,
23- stomata boss, the second circular holes of 24-, 25- face of weld, 26- round boss, the first hooks of 27-, 28- wedge grooves, 29- bayonets,
30- circle light transmission pieces, the first apertures of 31-.
Specific embodiment
The present invention is described in more detail below by specific embodiment, for a better understanding of the present invention,
But following embodiments are not intended to limit the scope of the invention.
Embodiment 1
As shown in figs. 1-7, the present embodiment provides a kind of chemical luminescence reagent kit of miniature more bins control, including：
Reagent box body A is made of, as shown in Figure 5 box main body A1, soft rubber cushion A2 and welding plate A3；On the box main body A1
If there are six liquid cabin 1,2,3,4,5,6, four shaft storehouses 9 of gas storehouse 8, one of reaction warehouse 7, one and a dryer bins
10, as shown in Figure 3-4；Each liquid cabin and 7 bottom of the reaction warehouse are equipped with the first aperture 31；7 bottom of reaction warehouse
Four first apertures 31 in portion are connected to by the groove 15 of bottom four with boss around the shaft storehouse 9, and with six
Six the first apertures 31 composition annular spread of 1,2,3,4,5,6 bottom of liquid cabin；Four 7 upper ends of reaction warehouse are also divided
Not She You groove 13, offer venthole 11 between the dryer bin 10 and gas storehouse 8, make the reaction warehouse 7 and 8 phase of gas storehouse
It is logical；
The shaft B of syringe-like structure in the shaft storehouse 9 is rotatably disposed in, as shown in Fig. 1, Fig. 6, by cavity
B1, push rod B2 and rubber plug B-3 are formed and are formed bin 17, and the cavity B1 is additionally provided with trepanning 19, the trepanning 19 can with it is described
Bin 17 connects；The push rod B2 upper ends are additionally provided with the second hook 18, and the rubber plug B3 is fixed to the push rod B2 bottoms；
It is set at the top of the reagent box body A and renovates C and side cover E, for sealing at the top of described dose of box body A；And
The bottom cover D of the reagent box body A bottoms is set to, for sealing described dose of box body A bottom.
As shown in figure 4, on the chemical luminescence reagent kit of miniature more bin controls of this implementation, 10 liang of the dryer bin
Side is additionally provided with fixture block 12, positioning use when the fixture block 12 is as welding light-transmitting plate F.And it is additionally provided in the box main body A1 bottoms
For fixing the card slot 14 of the bottom cover D.
As shown in figure 5, on the chemical luminescence reagent kit of miniature more bin controls of this implementation, the welding plate A3 is equipped with
Four with the 15 corresponding second orifice 16 of groove, the upper side of the welding plate A3 is equipped with the soft rubber cushion A2, when the weldering
After plate A3 is fixed to 1 bottom of main body A described in box by welding or other modes, the soft rubber cushion A2 and the ditch with boss
15 clamping of slot, which is engaged, to be formed four fluid passages and connects the aperture 16 with four through-holes 31 respectively with 7 bottom of reaction warehouse.
And the first through hole 31 of four second orifices 16 and six 1,2,3,4,5,6 bottoms of liquid cabin forms one group same
The annular spread hole of plane.
As shown in fig. 7, on the chemical luminescence reagent kit of miniature more bin controls of this implementation, the cavity B1 bottoms are also
Equipped with the second card slot 20, for the chemical luminescence reagent kit to be fixed to the driving of detection device by second card slot 20
On axis.
As shown in figure 8, on the chemical luminescence reagent kit of miniature more bin controls of this implementation, the C that renovates is by fixing
C1 and removable cover C2 compositions are covered, the fixed cover C1 is equipped with the first circular hole 21, adding mouth 22 and six with leachy stomata
Boss 23；The removable cover C2 be equipped with six 23 corresponding second circular holes 24 of stomata boss and face of weld 25, it is described
Sealed membrane G is laid on face of weld 25.
As shown in figure 9, on the chemical luminescence reagent kit of miniature more bin controls of this implementation, the bottom cover D is equipped with
Round boss 26 is arranged in the first hook 27 that 26 surrounding of round boss and the card slot 14 agree with and positioned at its bottom
Wedge groove 28.
As shown in Fig. 2,10, on the chemical luminescence reagent kit of miniature more bin controls of this implementation, including being set to
It states described in corresponding to there are four being set on the light-transmitting plate F, the light-transmitting plate F at dryer bin 10 and four 7 tops of reaction warehouse
The round light transmission piece 30 of reaction warehouse 7, both sides, which are equipped with, to be buckled in the bayonet 29, the bayonet 29 on the fixture block 12.When saturating
Tabula rasa F and box main body A1 welding is completed to form 4 stomatas between rearward recess 13 and light-transmitting plate, makes four reaction warehouses 7 and dryer bin
10 connections, dryer bin 10 are connected by venthole 11 with gas storehouse 8.
Embodiment 2
This implementation provides a kind of detection method of the chemical luminescence reagent kit of miniature more bin controls, has including walking as follows
Suddenly：
(1) side cover E is removed；
(2) as shown in figure 3,6 liquid cabins 1,2,3,4,5,6 being equipped in box main body A1, are welded in box main body A1 and head cover C
After connecing, reuse 6 that sealed membrane G is sealed on head cover C and carry stomata boss 23, make 6 liquid cabins and gas storehouse 8 closed, open
The removable cover C2 on C is renovated, sealed membrane G is made to be detached with leachy stomata boss 23；So that closed liquid cabin 1,2,3,
4th, 5,6 outdoor air is connected with gas storehouse 8；
(3) at sample application mouth 22,50~250 microlitres of serum or blood plasma are added in into liquid cabin 3, and cover removable cover
C2；
(4) kit is put into detection device along bottom wedge groove 28, the second card slot of shaft bottom 20 is made to be fixed to equipment
In drive shaft, while the second hook 18 that push rod B2 upper ends are equipped with enters the crawl position that upper side is arranged with, device drives
Shaft B is rotated, and trepanning 19 is made to be connected to box body A bin corresponding apertures position, and corresponding bin is made to connect to drive upside down again with shaft storehouse 9 and push away
Bar B2 and rubber plug B3 draws 50~200 microlitres of serum or blood plasma in liquid cabin 3, completes to extract liquid in bin 3；
(5) continue 6 corresponding hole of the liquid cabin position 31 that trepanning 19 is connected on box body A by rotating shaft B, by the blood of absorption
Cleer and peaceful blood plasma is mixed with the magnetic bead in liquid cabin 6；
(6) continue rotating shaft B, the magnetic bead serum or plasma pool that will be mixed in liquid cabin 6 in the same way
It is injected into dissolving ligand particle in the reaction warehouse 7 to be reacted, completes to react needed for test, as shown in figure 11；
(7) after reaction, the reaction liquid in reaction warehouse 7 is transferred to waste liquid storehouse-liquid cabin 1；It drives upside down and pushes away again
Bar B2 and rubber plug B3 completes the transfer or mixing of different bin liquid；
(8) cleaning solution cleans 1~3 time；
(9) finally, filling luminescent solution detection passes through the test in light-transmitting plate reading reaction warehouse by the scanning means of equipment
As a result.
The ligand particle, comprising：Antibody, antigen, haptens, polyclonal antibody, monoclonal antibody and hormone receptor etc..
Sample volume is 50~250 microlitres, preferably 200 microlitres in the present embodiment.Current embodiment require that a mating small chemical shines
Detector is used to implement the accurate transfer and control and photon detection of sample and liquid between multiple bins.The present embodiment can make
For the chemiluminescence based on magnetic particle, the chemiluminescence based on oligonucleotide marker can be used for.
The present embodiment chemical luminescence reagent kit be by the required component of detection process (magnetic bead liquid, cleaning solution, luminescent solution,
Antigen or antibody microballoon etc.) it is placed in specific storehouse.Reaction process is allocated each component mixing by project regulated procedure
And reaction.Comprising：Antigen-antibody combines, and forms sandwich structure, enrichment with magnetic bead and elution, the detection that shines, automation
It completes.
Bis- joint inspection project of embodiment 3CRP and PCT, the freeze-drying of antibody particle：
(1) buffer solution is lyophilized：20mM Tris, 5% trehalose, 20ng/ml caseins, 5% glucose, 2%PVP K30,
0.4%Tween 2.0,0.01%Prolin 300,0.1%AESF, pH value=7.5~8.0；
(2) anti-CRP the and anti-PCT antibody of biotin and azete ester will be marked respectively, and special ratios are dissolved in respectively
It is lyophilized in buffer solution.；
(3) it by the antibody of above-mentioned preparation, is placed in and is granulated on instrument (or manual), dripped drop with 30 or 50 microlitres of volumes per second
Enter in the incompatible liquid of ultralow temperature；Frozen particles are made；
(4) frozen particles shifting is fitted into bottle, is transferred quickly to be cooled in advance on -70 degree freeze dryer partition boards；
(5) it is dried in vacuo：- 70 degree, pre-freeze 2 hours；- 70 degree, 1~2 hour, vacuum degree was 0~500mTorr；- 50 degree,
24~36 hours, vacuum degree was 0~500mTorr；- 25 degree, 2~4 hours, vacuum degree was 0~500mTorr；0 degree, 1~2 is small
When, vacuum degree is 0~500mTorr；25 degree, 2~4 hours, vacuum degree was 0~500mTorr；After EP (end of program), manufacture；
(6) antibody particle should be the glossiness particle of white.
Application examples
1st, kit assembles：
(1) box body：
C bottoms fixed cover C1 will be renovated and be welded to kit main body A upper end, seal six 1,2,4,5,6,8 tops of bin
It closes, six bins are in communication with the outside respectively by six stomatas that fixed cover C1 is equipped with, and bin 3 is corresponding with adding mouth 22 to be connected
Logical, shaft storehouse 9 is corresponding with the first circular hole 21 with position；Then shaft B is inserted into from box body A bottoms in shaft storehouse 9, push rod B2
It reaches the second hook 18 across the first circular hole 21 and renovates C parallel positions, then bottom cover D is inserted in from box main body A lower ends, make bottom
14 clamping of the first card slot that four the first hooks 27 that lid D is equipped with are equipped with main body A 1, while round boss 26 withstands shaft B
Lower end will carry the soft rubber cushion A2 of the tight box main body A bottoms of face pressure of trepanning 19, form box body A bottoms closed on shaft B, when
When the trepanning 19 of shaft B turns to box body bottom and corresponds to hole site, box body A is that corresponding bin connects with the bin 17 on shaft B
It is logical, it can realize to extract by moving up and down push rod B2 again at this time and liquid in bin is corresponded in box body A or into bin
Injecting fluid；
The bayonet 29 that light-transmitting plate F is equipped with by both sides is positioned and is welded on box body A, make four round light transmission pieces 30 with
Reaction warehouse 7 corresponds to, and each bin forms air-tight state, is connected by venthole 11 with gas storehouse 8；
Then face of weld 25 and six are renovated at the top of C with leachy stomata boss by what sealed membrane G was fused to that lid gets togather
On 23, the stomata of stomata boss 23 is closed, completely encloses each liquid cabin 1,2,4,5,6,8 and reaction warehouse 7 in box body A,
Facilitate the storage and transport of liquid/solid reagent in liquid cabin and reaction warehouse；(2) reagent：Two bins are selected in reaction warehouse 7,
Each one of the antibody particle of biotin labeling and azete ester label anti-CRP are separately added into one reaction warehouse；Another reaction
Each one of the antibody particle of biotin labeling and azete ester label anti-CRP are added in storehouse；Liquid cabin 2 filling 700~1000 is micro-
The luminescent solution risen；The cleaning solution that 5 filling 2000~3000 microlitres of liquid cabin；The cleaning solution that 6 filling 100~500 microlitres of liquid cabin.
2nd, upper machine testing：
(1) side cover E is removed, opens upper cover C2, adds in 200 microlitres of serum；
(2) upper cover C2 is covered, puts kit into instrument；
(3) it detects.
3rd, interpretation of result：
(1) CRP and PCT lowest detections line is 1pg/ml；
(2) linearly dependent coefficient is more than 99%；
(3) stability that batch is shown in is fine, and CVs% is less than 1%.
Specific embodiments of the present invention are described in detail above, but it is intended only as example, it is of the invention and unlimited
It is formed on particular embodiments described above.To those skilled in the art, it is any to the equivalent modifications that carry out of the present invention and
It substitutes also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of chemical luminescence reagent kit of miniature more bin controls, which is characterized in that including：
Reagent box body (A) is made of box main body (A1), soft rubber cushion (A2) and welding plate (A3), and the box main body (A1) is equipped with
Six liquid cabins (1,2,3,4,5,6), four reaction warehouses (7), a gas storehouse (8), a shaft storehouse (9) and a drier
Storehouse (10)；Each liquid cabin and the reaction warehouse (7) bottom are equipped with the first aperture (31)；Reaction warehouse (7) bottom
Four the first apertures (31) be connected to around the shaft storehouse (9) by the groove (15) of bottom four with boss, and with
Six the first apertures (31) composition annular spread of six liquid cabin (1,2,3,4,5,6) bottoms；Four reaction warehouses
(7) upper end further respectively has groove (13), offers venthole (11) between the dryer bin (10) and gas storehouse (8), makes institute
Reaction warehouse (7) is stated to communicate with gas storehouse (8)；
The shaft (B) of the interior syringe-like structure of shaft storehouse (9) is rotatably disposed in, by cavity (B1), push rod (B2)
Formed with rubber plug (B3) and form bin (17), the cavity (B1) is additionally provided with trepanning (19), the trepanning (19) can with it is described
Bin (17) connects；Push rod (B2) upper end is additionally provided with the second hook (18), and the rubber plug (B3) is fixed to the push rod
(B2) bottom；
It is set at the top of the reagent box body (A) and renovates (C) and side cover (E), for sealing at the top of described dose of box body (A)；With
And
The bottom cover (D) of reagent box body (A) bottom is set to, for sealing described dose of box body (A) bottom.
2. the chemical luminescence reagent kit of miniature more bin controls according to claim 1, which is characterized in that the drier
Storehouse (10) both sides are additionally provided with fixture block (12), positioning use when the fixture block (12) is as welding light-transmitting plate (F).
3. the chemical luminescence reagent kit of miniature more bin controls according to claim 1, which is characterized in that the box main body
(A1) bottom is additionally provided with the card slot (14) for fixing the bottom cover (D).
4. the chemical luminescence reagent kit of miniature more bin controls according to claim 1, which is characterized in that the welding plate
(A3) second orifice (16) corresponding with the groove (15) there are four being set on, the upper side of the welding plate (A3) is equipped with described soft
Rubber cushion (A2), after the welding plate (A3) is fixed to the box main body (A1) bottom, the soft rubber cushion (A2) is with carrying boss
Groove (15) clamping engages to form four fluid passages by the aperture (16) and four with the reaction warehouse (7) bottom respectively
Through-hole (31) connects.
5. the chemical luminescence reagent kit of miniature more bins control according to claim 4, which is characterized in that four described the
The first through hole (31) of two apertures (16) and six liquid cabin (1,2,3,4,5,6) bottoms forms one group conplane
Annular spread hole.
6. the chemical luminescence reagent kit of miniature more bin controls according to claim 1, which is characterized in that the cavity
(B1) bottom is additionally provided with the second card slot (20), is fixed to the chemical luminescence reagent kit for passing through second card slot (20)
In the drive shaft of detection device.
7. the chemical luminescence reagent kit of miniature more bin controls according to claim 1, which is characterized in that described to renovate
(C) be made of fixed cover (C1) and removable cover (C2), the fixed cover (C1) be equipped with the first circular hole (21), adding mouth (22) and
Six with leachy stomata boss (23)；The removable cover (C2) is equipped with corresponding with six stomata boss (23) the
Two circular holes (24) and face of weld (25) are laid with sealed membrane (G) on the face of weld (25).
8. the chemical luminescence reagent kit of miniature more bin controls according to claim 2, which is characterized in that the bottom cover
(D) it is equipped with round boss (26), is arranged in the first hook that the round boss (26) surrounding is agreed with the card slot (14)
(27) and the wedge groove (28) positioned at its bottom.
9. the chemical luminescence reagent kit of miniature more bin controls according to claim 2, which is characterized in that including being set to
The light-transmitting plate (F) stated at the top of dryer bin (10) and four reaction warehouses (7), set on the light-transmitting plate (F) there are four
Round light transmission piece (30), both sides corresponding to the reaction warehouse (7) are equipped with to be buckled in the bayonet (29), the bayonet (29)
On the fixture block (12).
10. a kind of detection method of chemical luminescence reagent kit controlled such as the miniature more bins of claim 1-9 any one of them,
It is characterised in that it includes following steps：
(1) side cover (E) is removed；
(2) removable cover (C2) renovated on (C) is opened, sealed membrane (G) is made to be detached with leachy stomata boss (23)；
(3) at sample application mouth (22), 50~250 microlitres of serum or blood plasma are added in, and cover removable cover to liquid cabin (3) is interior
(C2)；
(4) rotating shaft (B) draws 50~200 microlitres of serum or blood plasma in liquid cabin (3)；
(5) continue rotating shaft (B) to mix the serum of absorption and blood plasma with the magnetic bead in liquid cabin (6)；
(6) continue rotating shaft (B) and the magnetic bead serum in liquid cabin (6) or plasma pool are injected into the reaction warehouse (7)
Middle dissolving ligand particle is reacted；
(7) after reaction, the reaction liquid in reaction warehouse (7) is transferred to waste liquid storehouse；
(8) cleaning solution cleans 1~3 time；
(9) filling luminescent solution detection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810061122.XA CN108226550B (en) | 2018-01-22 | 2018-01-22 | Miniature multi-bin controlled chemiluminescent kit and detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810061122.XA CN108226550B (en) | 2018-01-22 | 2018-01-22 | Miniature multi-bin controlled chemiluminescent kit and detection method thereof |
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| CN108226550B CN108226550B (en) | 2023-12-01 |
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| WO2020088476A1 (en) * | 2018-10-31 | 2020-05-07 | 江苏卓微生物科技有限公司 | Multi-orifice sample introduction device and multi-orifice sample introduction method |
| CN113528287A (en) * | 2020-04-16 | 2021-10-22 | 广东润鹏生物技术有限公司 | Sample processing device and PCR instrument |
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| Publication number | Publication date |
|---|---|
| CN108226550B (en) | 2023-12-01 |
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