CN114164091A - Molecular diagnostic sample processing system and control method - Google Patents
Molecular diagnostic sample processing system and control method Download PDFInfo
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- CN114164091A CN114164091A CN202111482238.9A CN202111482238A CN114164091A CN 114164091 A CN114164091 A CN 114164091A CN 202111482238 A CN202111482238 A CN 202111482238A CN 114164091 A CN114164091 A CN 114164091A
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- 238000012545 processing Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 85
- 238000012546 transfer Methods 0.000 claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 230000007246 mechanism Effects 0.000 claims abstract description 26
- 238000007789 sealing Methods 0.000 claims abstract description 18
- 239000002699 waste material Substances 0.000 claims description 4
- 238000003745 diagnosis Methods 0.000 abstract description 11
- 238000005516 engineering process Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 27
- 239000002994 raw material Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 230000006872 improvement Effects 0.000 description 3
- 238000007781 pre-processing Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000003028 elevating effect Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The present disclosure relates to the field of molecular diagnosis technologies, and in particular, to a molecular diagnosis sample processing system and a control method thereof. The molecular diagnostic sample processing system comprises a cartridge and a first operational module; the first operation module can operate a main valve body of a main liquid transfer valve of the card box, so that the main valve body is respectively communicated with a plurality of second chambers of the card box; the first operation module further comprises a first lifting device and a puncturing mechanism, the puncturing mechanism is installed at the driving end of the first lifting device, so that the first lifting device can drive the puncturing mechanism to move towards the card box, and the puncturing mechanism can puncture sealing films on the second cabins, so that the situation that when reagents in the second cabins are extracted, smooth flowing of the reagents is influenced due to the fact that pressure above the reagents is reduced, and the reagents are extracted smoothly is avoided.
Description
Technical Field
The present disclosure relates to the field of molecular diagnosis technologies, and in particular, to a molecular diagnosis sample processing system and a control method thereof.
Background
In the case of a cartridge-type molecular diagnostic platform, a plurality of chambers are formed in a cartridge for holding all raw materials required for detection purposes, including a sample and various reagents for processing the sample, and then the cartridge is operated such that the raw materials or intermediate products are transferred between the respective chambers of the cartridge in accordance with predetermined steps to perform a series of operations from sample pretreatment, nucleic acid purification, and further, filling of a PCR reaction solution in the cartridge. In the development process of practical application, the applicant found that the raw materials cannot be stored in the cartridge for a long time due to the unstable or volatile characteristics of part of the raw materials, and if the raw materials are directly placed in a sealed reagent tube or a sealed reagent chamber of the cartridge, the reagents in the chamber cannot flow out smoothly.
Disclosure of Invention
The invention aims to provide a molecular diagnosis sample processing system and a control method, so that raw materials can be smoothly transferred in a cartridge.
The invention provides a molecular diagnosis sample processing system, which comprises a card box and a first operation module; the card box is provided with a plurality of second cabins, and a main liquid transfer valve is movably connected on the card box; the plurality of second cabins are used for storing reagents, and sealing films cover the upper parts of the plurality of second cabins for storing the reagents respectively; the first operating module can be connected with a main valve body of the main liquid transfer valve and drives the main valve body to move so as to enable the main valve body to be communicated with one of the second chambers respectively; the first operation module comprises a first lifting device and a puncturing mechanism, the puncturing mechanism is installed at the driving end of the first lifting device, and the first lifting device can drive the puncturing mechanism to move towards the card box, so that the puncturing mechanism punctures the sealing films on the second cabins.
Further, the lancing mechanism includes a plurality of second lancing portions; the plurality of second puncturing parts correspond to the plurality of second cabins one by one, and the plurality of second puncturing parts are used for puncturing sealing films of the corresponding second cabins respectively.
Furthermore, a plurality of first cabins and reagent connecting pipes are arranged on the card box, and a plurality of reagent pipes of the reagent connecting pipes are correspondingly placed in the first cabins one by one; the puncture mechanism also comprises a plurality of first puncture parts, and the first puncture parts correspond to the reagent tubes one by one; the plurality of first puncture parts are used for puncturing the sealing films on the corresponding reagent tubes.
Furthermore, a pressing block is further arranged at the driving end of the first lifting device; the first lifting device can drive the pressing block to press the reagent connecting pipes so as to press the plurality of reagent pipes into the corresponding first cabins; the bottom parts of the first cabins are respectively provided with a third puncturing part, and the third puncturing parts are used for puncturing the bottom parts of the corresponding reagent tubes.
Further, the plurality of first puncture parts are arranged on the lower surface of the pressing block at intervals.
Furthermore, a main rotating assembly and an auxiliary rotating assembly are further mounted at the driving end of the first lifting device; the main valve body is rotationally connected with the card box, and the card box is also rotationally provided with an auxiliary liquid transfer valve; the first lifting device can drive the main rotating assembly and the auxiliary rotating assembly to move so that the main rotating assembly is connected with the main valve body of the main liquid transfer valve, and the auxiliary rotating assembly is connected with the auxiliary valve body of the auxiliary liquid transfer valve; the main rotating assembly is used for driving the main valve body to rotate, the auxiliary rotating assembly is used for driving the auxiliary valve body to rotate, so that the main valve body can be communicated with a sample cabin, a waste liquid cabin, a redissolution cabin, a plurality of first cabins or a plurality of second cabins of the card box, and the auxiliary valve body can be communicated with the redissolution cabin or a plurality of capillaries placed on the card box.
Further, the main rotating assembly and the auxiliary rotating assembly respectively comprise a rotating connecting piece and a first driving device; the rotating connecting piece is arranged in a rotating mode, and the first driving device can drive the rotating connecting piece to rotate; the rotary connecting piece is provided with a positioning pin, and positioning grooves are formed in the main valve body and the auxiliary valve body; the positioning pin can be inserted into the positioning groove of the corresponding main valve body or the auxiliary valve body.
Furthermore, a first gear is coaxially arranged on the rotary connecting piece, and a second gear is arranged at the driving end of the first driving device; the first driving device can drive the second gear to rotate, and the second gear is meshed with the first gear.
Furthermore, an origin positioning hole is formed in the first gear, a photoelectric switch is arranged on one side of the first gear in the radial direction, and the first gear can rotate to the position, opposite to the origin positioning hole, of the photoelectric switch so as to perform origin positioning on the main valve body or the auxiliary valve body.
Further, the molecular diagnostic sample processing system further comprises a second operation module and a third operation module; the second operation module and the third operation module are respectively arranged at two sides of the first operation module; the main liquid transfer valve and the auxiliary liquid transfer valve are piston valves, the second operation module is used for driving a main valve rod of the main liquid transfer valve to do piston motion, and the third operation module is used for driving an auxiliary valve rod of the auxiliary liquid transfer valve to do piston motion.
Further, the second operating module and the third operating module respectively comprise a second lifting device, a telescopic driving device and a valve rod connecting piece; the valve rod connecting piece is arranged at the driving end of the second lifting device through the telescopic driving device; the telescopic driving device is used for driving the valve rod connecting piece to extend towards the corresponding main valve rod or the corresponding auxiliary valve rod so as to enable the valve rod connecting piece to be connected with the corresponding main valve rod and the corresponding auxiliary valve rod; the second lifting device is used for driving the valve rod connecting piece to lift so as to drive the corresponding main valve rod or the corresponding auxiliary valve rod to lift.
Further, the valve rod connecting piece is fork-shaped; the main valve rod and the auxiliary valve rod are provided with slots, and the valve rod connecting piece can be inserted into the slots.
The application also provides a control method of the molecular diagnosis sample processing system, which comprises the following steps:
performing puncture pretreatment on a sealed reagent cabin of the card box;
the main valve body and/or the auxiliary valve body are controlled to rotate by a preset angle, so that the main valve body and/or the auxiliary valve body are communicated with the chamber in the cartridge according to a preset sequence, and the main valve body and the auxiliary valve body are controlled to move through the control valve rod, so that reagent is transferred between the main valve body and the auxiliary valve body and the communicated chamber.
Further, the control method of the molecular diagnostic sample processing system further comprises the following steps:
when the main valve body or the auxiliary valve body is rotated to be communicated with a preset chamber for the first time, the original point positioning is carried out on the main valve body or the auxiliary valve body.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a molecular diagnosis sample processing system, which comprises a card box and a first operation module; the card box is movably connected with a main liquid transfer valve, a plurality of second cabins used for placing various reagents are formed on the card box, and sealing films cover the second cabins so as to seal the reagents in the second cabins through the sealing films.
The first operation module can drive a main valve body of the main liquid transfer valve to rotate, so that the main valve body can be communicated with the plurality of second cabins respectively, the first operation module further comprises a first lifting device and a puncturing mechanism, the puncturing mechanism is installed at the driving end of the first lifting device, and the first lifting device can drive the puncturing mechanism to move towards the card box and puncture sealing films at the upper ends of the plurality of second cabins. Therefore, when the reagent in the second chamber is extracted, the smooth outflow of the reagent is prevented from being influenced by the fact that the pressure above the reagent is reduced, and the reagent is extracted smoothly.
The application also provides a control method of the molecular diagnosis sample processing system, which comprises the following steps: the rotation sequence and the rotation angle of the main valve body and the auxiliary valve body are controlled to enable the main valve body and the auxiliary valve body to be communicated with the chamber on the card box according to a preset sequence, and the reagent transfer of the main valve body and the auxiliary valve body and the communicated chamber part is realized through the control valve rod, such as the reagent is pushed into the communicated chamber or is extracted from the communicated chamber. When the main valve body and the auxiliary valve body are rotated to be communicated with a preset cabin each time, the main valve body and the auxiliary valve body are firstly positioned at the original points, and the main valve body and the auxiliary valve body are rotated to the original point positions, so that the main valve body and the auxiliary valve body can be accurately rotated by preset angles to be communicated with the required cabin.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a cartridge provided in an embodiment of the present invention;
FIG. 2 is a schematic diagram of a molecular diagnostic sample processing system according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a first operation module according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a lancing mechanism of a first manipulator module according to an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of a main rotating assembly of a first operating module according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a second operation module according to an embodiment of the present invention.
Reference numerals:
1-a first operating module, 11-a first lifting device, 12-a mounting plate, 13-a press block, 14-a first puncturing part, 15-a second puncturing part, 16-a main rotating assembly, 161-a first driving device, 162-a second gear, 163-a first gear, 164-a rotating connecting piece, 165-a positioning pin, 166-an origin positioning hole, 167-an optoelectronic switch, and 17-an auxiliary rotating assembly;
2-a second operating module, 21-a second lifting device, 22-a telescopic driving device, 23-a valve rod connecting piece;
3-a third operational module;
4-cartridge, 41-main pipetting valve, 411-main valve body, 412-main valve stem, 413-positioning slot, 414-slot, 42-auxiliary pipetting valve, 43-reagent union, 44-second chamber.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.
The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Molecular diagnostic sample processing systems and control methods according to some embodiments of the present application are described below with reference to fig. 1-6.
Example one
The present application provides a molecular diagnostic sample processing system, as shown in fig. 1 and 2, comprising a cartridge 4, and a second manipulator module 2, a first manipulator module 1 and a third manipulator module 3, which are arranged side by side.
The cartridge 4 is a prior art cartridge 4, as shown in fig. 1, a plurality of chambers are formed in the cartridge 4 for placing various raw materials, including a sample chamber for placing a sample, a plurality of first chambers for placing various reagents, a plurality of second chambers 44 and a reconstitution chamber, and a waste liquid chamber, and a plurality of capillaries containing specific primers and probes are further disposed on the cartridge 4; the main pipetting valve 41 and the sub pipetting valve 42 on the cartridge 4 are operated to transfer the raw materials in the cartridge 4 between the chambers in a predetermined order to complete the pretreatment of the sample, and the resulting sample treatment liquid is filled into the capillary. The main pipetting valve 41 and the auxiliary pipetting valve 42 are respectively connected to the card box 4 in a rotating way, and the main pipetting valve and the auxiliary pipetting valve can be respectively communicated with different cabins by rotating valve bodies of the main pipetting valve and the auxiliary pipetting valve, specifically, the main pipetting valve 41 can be communicated with a sample cabin, a waste liquid cabin, a re-dissolving cabin, a plurality of first cabins and a plurality of second cabins 44, and the auxiliary pipetting valve 42 can be communicated with the re-dissolving cabin and a plurality of capillaries; the main liquid transfer valve 41 and the auxiliary liquid transfer valve 42 are piston valves, and the materials between the main liquid transfer valve and the auxiliary liquid transfer valve and the communicated cabins can be transferred by pushing the valve rod into the valve body or pulling the valve rod out of the valve body.
When the cartridge 4 is placed at the preprocessing station, specifically, a moving mechanism may be provided to move the cartridge 4 to the preprocessing station; the first operating module 1 can drive the main valve body 411 of the main pipetting valve 41 and the auxiliary valve body of the auxiliary pipetting valve 42 to rotate, so that the main pipetting valve 41 and the auxiliary pipetting valve 42 can be communicated with different chambers; the second operation module 2 can drive the valve rod of the main liquid-transferring valve 41, namely the main valve rod 412 to lift so as to enable the main valve rod 412 to do piston motion in the main valve body 411, and the third operation module 3 can drive the valve rod of the auxiliary liquid-transferring valve 42, namely the auxiliary valve rod to lift so as to enable the auxiliary valve rod to do piston motion in the auxiliary valve body, so that material transfer between the main liquid-transferring valve and the auxiliary liquid-transferring valve and communicated cabins is realized; furthermore, the full-automatic operation of the card box 4 can be realized by arranging the first operation module 1, the second operation module 2 and the third operation module 3 so as to complete the pretreatment and capillary filling operation of the sample, so that the operation efficiency and the operation precision are greatly improved compared with manual operation, and the time required by molecular diagnosis is shortened.
Example two
The second embodiment is an improvement on the basis of the first embodiment, technical contents disclosed in the first embodiment are not described repeatedly, and contents disclosed in the first embodiment also belong to contents disclosed in the first embodiment.
The card box 4 is provided with a reagent connecting tube 43, as shown in fig. 1, the reagent connecting tube 43 includes a plurality of reagent tubes, different reagents are respectively packaged in the plurality of reagent tubes, and the plurality of reagent tubes are correspondingly placed in the plurality of first chambers of the card box 4 one by one. The bottom of the first chamber is formed with a third piercing part toward the inside of the chamber, and when the reagent in the reagent tube needs to be used, a downward pressure needs to be applied to the reagent connecting tube 43 so that the third piercing part can pierce the corresponding reagent tube.
In the second embodiment, preferably, as shown in fig. 3 and 4, the first operation module 1 includes a first lifting device 11 and a puncturing mechanism, the driving end of the first lifting device 11 is provided with a mounting plate 12, the first lifting device 11 can drive the mounting plate 12 to lift and lower in the vertical direction, and the preprocessing station of the cartridge 4 is located below the mounting plate 12; the puncturing mechanism comprises a pressing block 13, the pressing block 13 is arranged on the lower plate surface of the mounting plate 12 facing the card box 4, and the pressing block 13 is positioned right above the reagent connecting pipe 43; when the mounting plate 12 is lowered to a predetermined position, the pressing block 13 can be pressed against the reagent connecting tube 43, so that the reagent tube is tightly pressed into the corresponding first chamber, the bottom of the reagent tube is pierced by the third piercing part, and the reagent tube is communicated with the corresponding first chamber, and the main pipette valve 41 can draw the reagent from the reagent tube.
In this embodiment, preferably, as shown in fig. 4, the piercing mechanism further includes a plurality of first piercing portions 14, and the plurality of first piercing portions 14 correspond to the plurality of first compartments one to one; the plurality of first piercing portions 14 are provided on the pressing block 13, and the plurality of first piercing portions 14 are respectively located right above the corresponding first chamber, so that when the mounting plate 12 is lowered to a predetermined position to press the pressing block 13 against the reagent connecting tube 43, not only the bottom of the reagent tube can be pierced by the third piercing portion, but also the plurality of first piercing portions 14 can respectively pierce the sealing film at the upper end of the reagent tube in the corresponding first chamber, thereby preventing the smooth outflow of the reagent from being affected by the reduction in pressure above the reagent in the reagent tube when the main pipetting valve 41 withdraws the reagent in the reagent tube, and further smoothly completing the reagent withdrawal.
In this embodiment, the plurality of second chambers 44 of the cartridge 4 are covered with sealing films to seal the reagents in the second chambers 44 by the sealing films, and in order to enable the reagents in the second chambers 44 to be smoothly extracted by the main liquid transfer valve 41, preferably, as shown in fig. 4, the puncturing mechanism further includes a plurality of second puncturing parts 15, the plurality of second puncturing parts 15 correspond to the plurality of second chambers 44 one by one, the plurality of second puncturing parts 15 are mounted on the mounting plate 12 and respectively located right above the corresponding second chambers 44, and when the mounting plate 12 is lowered to a predetermined position, the plurality of second puncturing parts 15 can puncture the sealing films on the corresponding second chambers 44, so as to enable the reagents in the second chambers 44 to flow out smoothly.
EXAMPLE III
The third embodiment is an improvement on the above embodiment, technical contents disclosed in the above embodiment are not described repeatedly, and the contents disclosed in the above embodiment also belong to the disclosure of the embodiment.
In the third embodiment, preferably, as shown in fig. 3, the first operating module 1 further includes a main rotating assembly 16 and a sub rotating assembly 17, and the main rotating assembly 16 and the sub rotating assembly 17 are respectively mounted on the mounting plate 12 of the first elevating device 11 to be capable of elevating with the mounting plate 12. When the mounting plate 12 is lowered to a predetermined position, the main rotating assembly 16 can be connected to the main valve body 411 of the main pipetting valve 41, the sub rotating assembly 17 can be connected to the sub valve body 411 of the sub pipetting valve 42, the main rotating assembly 16 is used for driving the main valve body 411 to rotate, the sub rotating assembly 17 is used for driving the sub valve body to rotate, and therefore the main pipetting valve 41 and the sub pipetting valve 42 can be respectively communicated with different chambers by rotating the main valve body 411 and the sub valve body by different angles.
The main rotor assembly 16 and the sub-rotor assembly 17 have the same structure, and the structure of the main rotor assembly 16 and the sub-rotor assembly 17 will be described below by taking the main rotor assembly 16 as an example.
In this embodiment, preferably, as shown in fig. 5, the main rotating assembly 16 includes a rotating link 164 and a first driving device 161, wherein the rotating link 164 is rotatably connected to the mounting plate 12 in a vertical direction so that the rotating link 164 can rotate about its axis; the first driving device 161 is fixedly installed on the mounting plate 12, and the driving end of the first driving device 161 is connected with the rotary connector 164, so that the first driving device 161 can drive the rotary connector 164 to rotate.
The rotary connector 164 is disposed coaxially with the main valve body 411, and when the mounting plate 12 is lowered to a predetermined position, the lower end of the rotary connector 164 can be connected to the main valve body 411, so that the rotary connector 164 can drive the main valve body 411 to rotate synchronously. Preferably, the lower end of the rotary connector 164 is provided with a positioning pin 165, the side wall of the main valve body 411 is provided with a positioning groove 413, and the positioning pin 165 can be inserted into the positioning groove 413, so that the rotary connector 164 is connected with the main valve body 411, and the main valve body 411 can synchronously rotate along with the rotary connector 164.
Preferably, as shown in fig. 1 and 5, the number of the positioning grooves 413 is plural, and the plural positioning grooves 413 are distributed at intervals along the circumferential direction of the main valve body 411; the number of the positioning pins 165 is also a plurality of, and a plurality of positioning pins 165 are distributed along the circumference interval of the rotating connector 164, and a plurality of positioning pins 165 and a plurality of positioning grooves 413 are in one-to-one correspondence, and a plurality of positioning pins 165 can be respectively inserted into a plurality of corresponding positioning grooves 413, so that the rotating connector 164 is more stable in connection with the main valve body 411, and the main valve body 411 can be stably rotated under the driving of the rotating connector 164.
In this embodiment, preferably, as shown in fig. 5, a first gear 163 is coaxially disposed on the rotary connector 164, the driving end of the first driving device 161 is provided with a second gear 162, and the second gear 162 is engaged with the first gear 163; the first driving device 161 can drive the second gear 162 to rotate, and further drive the first gear 163 and the rotating link 164 to rotate.
In this embodiment, as shown in fig. 5, preferably, an origin positioning hole 166 is opened at the peripheral edge of the first gear 163, a photoelectric switch 167 is disposed at a position on the mounting plate 12 close to the first gear 163, and the peripheral edge of the first gear 163 extends into the sensing area of the photoelectric switch 167; the main valve body 411 has an initial origin position, and when the main valve body 411 is located at the origin position, the origin positioning hole 166 on the first gear 163 is opposite to the photoelectric switch 167, so as to perform origin positioning on the main valve body 411 through the origin positioning hole 166; the main valve body 411 can then be rotated to different angles to communicate with different chambers based on the home position.
In actual operation, the main valve body is initially positioned at an original position by the original position positioning hole 166, and then the main valve body 411 is driven by the first driving device 161 to rotate by a predetermined angle to be sequentially communicated with a plurality of chambers for material transfer.
The structure of the auxiliary rotating assembly 17 and the rotation driving of the auxiliary valve body by the auxiliary rotating assembly 17 are the same, and are not described in detail herein.
Example four
The fourth embodiment is an improvement on the basis of the first embodiment, technical contents disclosed in the first embodiment are not described repeatedly, and contents disclosed in the first embodiment also belong to the first embodiment.
In the fourth embodiment, as shown in fig. 2, the second operation module 2 and the third operation module 3 are respectively disposed on two sides of the first operation module 1, and the second operation module 2 is used to drive the main valve stem 412 to move up and down, so that the main valve stem 412 performs a piston motion in the main valve body 411, so that the main pipetting valve 41 can draw reagent from the chamber communicated with it or push the reagent in the main pipetting valve 41 into the chamber communicated with it, thereby realizing material transfer between the main pipetting valve 41 and the chamber communicated with it. The third operating module 3 is used for driving the auxiliary valve rod to lift and lower, so that the auxiliary valve rod does piston motion in the auxiliary valve body, and reagent transfer between the auxiliary liquid-transfering valve 42 and the communicated cabin is realized.
The second manipulator module 2 and the third manipulator module 3 have the same structure, and the structure of the second manipulator module 2 and the third manipulator module 3 will be described below by taking the second manipulator module 2 as an example.
Preferably, as shown in fig. 6, the second operating module 2 includes a valve rod connector 23, a second lifting device 21 and a telescopic driving device 22, the telescopic driving assembly is mounted at a driving end of the second lifting device 21, the valve rod connector 23 is mounted at a driving end of the telescopic driving device 22, so that the second lifting device 21 can drive the valve rod connector 23 to lift in a vertical direction, and the telescopic driving device 22 can drive the valve rod connector 23 to move in a horizontal direction.
Preferably, the stem connecting member 23 is fork-shaped, a slot 414 is formed in a side wall of the main valve stem 412, when the second lifting device 21 drives the stem to move to a predetermined height along a vertical direction, the stem connecting member 23 and the slot 414 are located at the same height, and then the stem connecting member 23 is driven by the telescopic driving device 22 to move towards the main valve stem 412 along a horizontal direction, so that the main valve stem 412 extends between two prongs of the stem connecting member 23, and the two prongs of the stem connecting member 23 are inserted into the slot 414 of the main valve stem 412, thereby connecting the stem connecting member 23 and the main valve stem 412.
After the valve rod connector 23 is inserted into the main valve rod 412, the valve rod connector 23 is driven by the second lifting device 21 to lift along the vertical direction, so that the main valve rod 412 can lift along the vertical direction, and the main valve rod 412 performs piston motion in the main valve body 411, thereby realizing reagent transfer between the main pipetting valve 41 and a communicated chamber.
The third operation module 3 has the same structure as the second operation module 2, and is not described herein again.
EXAMPLE five
In a fifth embodiment, a control method of the molecular diagnostic sample processing system according to the above-described embodiment is provided, in which the main pipetting valve and the sub-pipetting valve are configured to transfer the reagents in the cartridge in a predetermined order, the sample and the reagents are reacted in a predetermined order, and the sample processing liquid after the reaction is filled in the capillary.
Specifically, the control method of the molecular diagnosis sample processing system comprises the following steps:
the method comprises the steps of firstly performing puncture pretreatment on a sealed reagent cavity on a card box through a puncture mechanism, specifically, pressing down a reagent connecting pipe into a first cabin through a pressing block to puncture the lower ends of a plurality of reagent pipes of the reagent connecting pipe through a third puncture part on the bottom wall of the first cabin, simultaneously puncturing sealing films at the upper ends of the plurality of reagent pipes through the first puncture part, and puncturing sealing films on a plurality of second cabins through a second puncture part.
And then controlling the main valve body and/or the auxiliary valve body to rotate by a preset angle, so that the main valve body and the auxiliary valve body are communicated with the chamber on the cartridge according to a preset sequence, and realizing reagent transfer between the main valve body and the auxiliary valve body and the communicated chamber part through a control valve rod, such as pushing the reagent into the communicated chamber or extracting the reagent from the communicated chamber.
When the main valve body or the auxiliary valve body is rotated to be communicated with a preset cabin for the first time, the main valve body or the auxiliary valve body is firstly positioned at the original point, and the main valve body and the auxiliary valve body are rotated to the original point position, so that the main valve body and the auxiliary valve body can be accurately rotated by a preset angle to be communicated with the required cabin.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (14)
1. A molecular diagnostic sample processing system, comprising a cartridge and a first manipulator module;
the card box is provided with a plurality of second cabins, and a main liquid transfer valve is movably connected on the card box;
the plurality of second cabins are used for storing reagents, and sealing films cover the upper parts of the plurality of second cabins for storing the reagents respectively;
the first operating module can be connected with a main valve body of the main liquid transfer valve and drives the main valve body to move so as to enable the main valve body to be communicated with one of the second chambers respectively;
the first operation module comprises a first lifting device and a puncturing mechanism, the puncturing mechanism is installed at the driving end of the first lifting device, and the first lifting device can drive the puncturing mechanism to move towards the card box, so that the puncturing mechanism punctures the sealing films on the second cabins.
2. The molecular diagnostic sample processing system of claim 1, wherein the puncturing mechanism comprises a plurality of second punctures;
the plurality of second puncturing parts correspond to the plurality of second cabins one by one, and the plurality of second puncturing parts are used for puncturing sealing films of the corresponding second cabins respectively.
3. The molecular diagnostic sample processing system according to claim 1, wherein a plurality of first chambers and reagent headers are provided on the cartridge, and a plurality of reagent tubes of the reagent headers are placed in the plurality of first chambers in a one-to-one correspondence;
the puncture mechanism also comprises a plurality of first puncture parts, and the first puncture parts correspond to the reagent tubes one by one;
the plurality of first puncture parts are used for puncturing the sealing films on the corresponding reagent tubes.
4. The molecular diagnostic sample processing system of claim 3, wherein the drive end of the first lifting device is further provided with a press block;
the first lifting device can drive the pressing block to press the reagent connecting pipes so as to press the plurality of reagent pipes into the corresponding first cabins;
the bottom parts of the first cabins are respectively provided with a third puncturing part, and the third puncturing parts are used for puncturing the bottom parts of the corresponding reagent tubes.
5. The molecular diagnostic sample processing system of claim 4, wherein the plurality of first punctures are disposed at intervals on a lower surface of the compact.
6. The molecular diagnostic sample processing system of claim 3, wherein the drive end of the first lifting device further mounts a primary rotation assembly and a secondary rotation assembly;
the main valve body is rotationally connected with the card box, and the card box is also rotationally provided with an auxiliary liquid transfer valve;
the first lifting device can drive the main rotating assembly and the auxiliary rotating assembly to move so that the main rotating assembly is connected with the main valve body of the main liquid transfer valve, and the auxiliary rotating assembly is connected with the auxiliary valve body of the auxiliary liquid transfer valve;
the main rotating assembly is used for driving the main valve body to rotate so that the main valve body can be communicated with a sample cabin, a waste liquid cabin, a redissolution cabin, a plurality of first cabins or a plurality of second cabins of the cartridge; the auxiliary rotating assembly is used for driving the auxiliary valve body to rotate, so that the auxiliary valve body can be communicated with the redissolution cabin or a plurality of capillaries arranged on the card box.
7. The molecular diagnostic sample processing system of claim 6, wherein the primary rotation assembly and the secondary rotation assembly each comprise a rotational connection and a first drive device;
the rotating connecting piece is arranged in a rotating mode, and the first driving device can drive the rotating connecting piece to rotate;
the rotary connecting piece is provided with a positioning pin, and positioning grooves are formed in the main valve body and the auxiliary valve body;
the positioning pin can be inserted into the positioning groove of the corresponding main valve body or the auxiliary valve body.
8. The molecular diagnostic sample processing system of claim 7, wherein the rotational connector has a first gear coaxially disposed thereon, and the first drive device has a second gear disposed at a drive end thereof;
the first driving device can drive the second gear to rotate, and the second gear is meshed with the first gear.
9. The system of claim 8, wherein the first gear has an origin positioning hole, and a photoelectric switch is disposed on one side of the first gear in the radial direction, and the first gear can rotate until the origin positioning hole is opposite to the photoelectric switch, so as to perform origin positioning on the main valve body or the auxiliary valve body.
10. The molecular diagnostic sample processing system of claim 6, further comprising a second operational module and a third operational module;
the second operation module and the third operation module are respectively arranged at two sides of the first operation module;
the main liquid transfer valve and the auxiliary liquid transfer valve are piston valves, the second operation module is used for driving a main valve rod of the main liquid transfer valve to do piston motion, and the third operation module is used for driving an auxiliary valve rod of the auxiliary liquid transfer valve to do piston motion.
11. The molecular diagnostic sample processing system of claim 10, wherein the second operational module and the third operational module each comprise a second lifting device, a telescopic drive, and a valve stem connector;
the valve rod connecting piece is arranged at the driving end of the second lifting device through the telescopic driving device;
the telescopic driving device is used for driving the valve rod connecting piece to extend towards the corresponding main valve rod or the corresponding auxiliary valve rod so as to enable the valve rod connecting piece to be connected with the corresponding main valve rod and the corresponding auxiliary valve rod;
the second lifting device is used for driving the valve rod connecting piece to lift so as to drive the corresponding main valve rod or the corresponding auxiliary valve rod to lift.
12. The molecular diagnostic sample processing system of claim 11, wherein the valve stem connector is forked;
the main valve rod and the auxiliary valve rod are provided with slots, and the valve rod connecting piece can be inserted into the slots.
13. A method of controlling a molecular diagnostic sample processing system, comprising the steps of:
performing puncture pretreatment on a sealed reagent cabin of the card box;
the main valve body and/or the auxiliary valve body are controlled to rotate by a preset angle, so that the main valve body and/or the auxiliary valve body are communicated with the chamber in the cartridge according to a preset sequence, and the main valve body and the auxiliary valve body are controlled to move through the control valve rod, so that reagent is transferred between the main valve body and the auxiliary valve body and the communicated chamber.
14. The method of controlling a molecular diagnostic sample processing system of claim 13, further comprising the steps of:
when the main valve body or the auxiliary valve body is rotated to be communicated with a preset chamber for the first time, the original point positioning is carried out on the main valve body or the auxiliary valve body.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111482238.9A CN114164091A (en) | 2021-12-07 | 2021-12-07 | Molecular diagnostic sample processing system and control method |
| PCT/CN2022/132639 WO2023103738A1 (en) | 2021-12-07 | 2022-11-17 | Molecular diagnostic sample processing system and control method |
| CN202223214610.0U CN218951399U (en) | 2021-12-07 | 2022-12-01 | Molecular diagnostic sample processing system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111482238.9A CN114164091A (en) | 2021-12-07 | 2021-12-07 | Molecular diagnostic sample processing system and control method |
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| CN114164091A true CN114164091A (en) | 2022-03-11 |
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| CN202223214610.0U Active CN218951399U (en) | 2021-12-07 | 2022-12-01 | Molecular diagnostic sample processing system |
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| WO (1) | WO2023103738A1 (en) |
Cited By (1)
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| WO2023103738A1 (en) * | 2021-12-07 | 2023-06-15 | 广东润鹏生物技术有限公司 | Molecular diagnostic sample processing system and control method |
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| CN218951399U (en) | 2023-05-02 |
| WO2023103738A1 (en) | 2023-06-15 |
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