WO2023103738A1

WO2023103738A1 - Molecular diagnostic sample processing system and control method

Info

Publication number: WO2023103738A1
Application number: PCT/CN2022/132639
Authority: WO
Inventors: 刘建知; 黄宏坤; 李晓峰; 张涛
Original assignee: 广东润鹏生物技术有限公司
Priority date: 2021-12-07
Filing date: 2022-11-17
Publication date: 2023-06-15
Also published as: CN114164091A; CN218951399U

Abstract

A molecular diagnostic sample processing system, comprising a cartridge and a first operation module, wherein the first operation module can operate a main valve body of a main liquid transfer valve of the cartridge to allow the main valve body to be respectively in communication with multiple second compartments of the cartridge; and the first operation module further comprises a first lifting device and a piercing mechanism which is mounted on a drive end of the first lifting device. The first lifting device can move the piercing mechanism towards the cartridge, and the piercing mechanism can pierce sealing films on the multiple second compartments, which avoids affecting the smooth outflow of a reagent due to the decrease of the pressure above the reagent when the reagent in the second compartments is extracted, so as to smoothly extract the reagent.

Description

Molecular diagnostic sample processing system and control method

Cross References to Related Applications

This disclosure claims priority to a Chinese patent application with application number 202111482238.9 titled "Molecular Diagnostic Sample Processing System and Control Method" filed with the China Patent Office on December 7, 2021, the entire contents of which are incorporated by reference in this disclosure .

technical field

The present disclosure relates to the technical field of molecular diagnosis, in particular to a molecular diagnosis sample processing system and control method.

Background technique

This section provides background information related to the present disclosure which may not necessarily constitute prior art.

For the cartridge-type molecular diagnostic platform, multiple compartments are formed in the cartridge to place all the raw materials required for detection purposes, including samples and various reagents for sample processing, and then operate the cartridge to make the raw materials or intermediate products According to the predetermined steps, it is transferred between the compartments of the cartridge, so as to complete a series of operations in the cartridge from sample pretreatment to nucleic acid purification, and then to filling of PCR reaction solution. During the actual application research and development process, the applicant found that due to the unstable or volatile characteristics of some raw materials, they cannot be stored in the cartridge for a long time. If the raw materials are directly placed in a sealed reagent tube, or placed in a cartridge In the sealed reagent compartment of the cartridge, the reagents in the compartment cannot flow out smoothly.

Contents of the invention

The purpose of the present disclosure is to provide a molecular diagnosis sample processing system and control method, so that the raw materials can be smoothly transferred in the cartridge.

An embodiment of the present disclosure provides a molecular diagnostic sample processing system, which may include a cartridge and a first operating module; the cartridge may be formed with a plurality of second chambers, and the cartridge may be movably connected to a main pipette valve; the plurality of second compartments can be configured to store reagents, and the tops of the plurality of second compartments storing reagents can be respectively covered with sealing films; the first operation module can be connected with the main pipetting valve The main valve body is connected and drives the main valve body to move, so that the main valve body communicates with one of the plurality of second compartments respectively; the first operating module may include a first lifting device and A piercing mechanism, the piercing mechanism can be installed on the driving end of the first lifting device, and the first lifting device can drive the piercing mechanism to move toward the cartridge, so that the piercing mechanism pierces Break the seal on a plurality of the second compartments.

Optionally, the piercing mechanism may include a plurality of second piercing parts; a plurality of second piercing parts may correspond to a plurality of second compartments one-to-one, and a plurality of second piercing parts may be configured to puncture a respective respective sealing membrane of said second compartment.

Optionally, the cartridge may be provided with multiple first compartments and reagent manifolds, and the reagent tubes of the reagent manifolds may be placed in multiple first compartments in a one-to-one correspondence; The piercing mechanism may also include a plurality of first piercing parts, and a plurality of the first piercing parts may correspond to a plurality of the reagent tubes one by one; a plurality of the first piercing parts may be configured as Pierce the seal on the corresponding reagent tube.

Optionally, the driving end of the first lifting device can also be provided with a pressing block; the first lifting device can drive the pressing block to press against the reagent tubes, so as to direct a plurality of the reagent tubes toward the corresponding The bottoms of a plurality of the first chambers may be respectively provided with third piercing parts, and the third piercing parts may be configured to pierce the bottoms of the corresponding reagent tubes .

Optionally, a plurality of the first piercing parts may be disposed on the pressing block, and the multiple first piercing parts are respectively located directly above the corresponding first compartments.

Optionally, a plurality of the first piercing portions may be arranged at intervals on the lower surface of the pressing block.

Optionally, the driving end of the first lifting device can also be equipped with a main rotating assembly and an auxiliary rotating assembly; Pipetting valve: the first lifting device can drive the main rotating assembly and the auxiliary rotating assembly to move, so that the main rotating assembly can be connected with the main valve body of the main pipetting valve, and the auxiliary rotating assembly The rotation assembly can be connected with the auxiliary valve body of the auxiliary pipetting valve; the main rotation assembly can be configured to drive the rotation of the main valve body, and the auxiliary rotation assembly can be configured to drive the rotation of the auxiliary valve body so that the main valve body can communicate with the sample compartment, the waste liquid compartment, the reconstitution compartment, a plurality of the first compartments or a plurality of the second compartments of the cartridge, and the auxiliary valve body It can communicate with the reconstitution chamber or a plurality of capillaries placed on the cassette.

Optionally, both the main rotating assembly and the auxiliary rotating assembly may include a rotating connector and a first driving device; the rotating connecting member may be rotatably arranged, and the first driving device may drive the rotating connecting member to rotate The rotating connector can be provided with a positioning pin, and both the main valve body and the auxiliary valve body can be provided with a positioning groove; the positioning pin can be plugged into the corresponding main valve body or the auxiliary valve body. In the positioning groove of the valve body.

Optionally, the driving end of the first lifting device may be provided with a mounting plate, and the rotating link is connected to the mounting plate in a rotatable manner along the vertical direction, so that the rotating link can rotate around itself The axis rotates; the first driving device is fixedly installed on the mounting plate, and the driving end of the first driving device is connected to the rotating connection to drive the rotating connecting part to rotate.

Optionally, the rotating connecting piece may be arranged coaxially with the main valve body, and the rotating connecting piece is configured such that when the installation plate is lowered to a predetermined position, the lower end of the rotating connecting piece can be connected to the The main valve bodies are connected so that the rotating connecting piece drives the main valve bodies to rotate synchronously.

Optionally, a plurality of positioning pins may be distributed at intervals along the circumferential direction of the rotating connector, and the plurality of positioning pins correspond to the plurality of positioning grooves one by one.

Optionally, a first gear may be coaxially provided on the rotating connector, and a second gear may be provided at the driving end of the first driving device; the first driving device can drive the second gear to rotate, The second gear may mesh with the first gear.

Optionally, an origin positioning hole may be provided on the first gear, a photoelectric switch may be provided on one side of the first gear in the radial direction, and the first gear can be rotated until the origin positioning hole is aligned with the origin positioning hole. The photoelectric switch is opposite to each other, so as to locate the origin of the main valve body or the auxiliary valve body.

Optionally, the molecular diagnostic sample processing system may further include a second operating module and a third operating module; the second operating module and the third operating module may be respectively arranged on two sides of the first operating module. side; both the main pipetting valve and the auxiliary pipetting valve can be piston valves, the second operating module can be configured to drive the main valve rod of the main pipetting valve to make a piston movement, and the third The operation module may be configured to drive the auxiliary valve stem of the auxiliary pipetting valve to make a piston movement.

Optionally, both the second operating module and the third operating module may include a second lifting device, a telescopic driving device, and a valve stem connecting piece; the valve stem connecting piece may be installed on the The driving end of the second lifting device; the telescopic driving device can be configured to drive the valve stem connecting piece to extend toward the corresponding main valve stem or the auxiliary valve stem, so that the valve stem connecting piece It is connected with the corresponding main valve stem and the auxiliary valve stem; the second lifting device can be configured to drive the valve stem connecting piece to lift to drive the corresponding main valve stem or the auxiliary valve Rod lift.

Optionally, the valve stem connecting piece may be in the shape of a fork; both the main valve stem and the auxiliary valve stem may be provided with slots, and the valve stem connecting piece can be inserted into the slots.

An embodiment of the present disclosure also provides a control method for a molecular diagnostic sample processing system, which may include the following steps:

Perform puncture pretreatment on the sealed reagent compartment of the cartridge;

Control the rotation of the main valve body and/or the auxiliary valve body to a predetermined angle, so that the main valve body and/or the auxiliary valve body are connected with the compartments in the cartridge according to a predetermined order, and the main and auxiliary valve bodies are realized by controlling the movement of the valve stem. Reagent transfer with compartment pieces in communication.

Optionally, the control method of the molecular diagnostic sample processing system may also include the following steps:

When the main valve body or the auxiliary valve body is turned to communicate with the predetermined compartment for the first time, the origin of the main valve body or the auxiliary valve body is positioned.

Compared with related technologies, the beneficial effects of the present disclosure can be:

A sample processing system for molecular diagnosis provided by an embodiment of the present disclosure includes a cartridge and a first operation module; The compartments, the plurality of second compartments are covered with sealing films, so as to seal the reagents in the second compartments through the sealing films.

The first operating module can drive the main valve body of the main pipetting valve to rotate, so that the main valve body can communicate with a plurality of the second chambers respectively, and the first operating module also includes a first lifting device and a piercing mechanism, and the piercing mechanism The breaking mechanism is installed on the driving end of the first lifting device, and the first lifting device can drive the piercing mechanism to move toward the cartridge, and puncture the sealing films at the upper ends of the plurality of second compartments. Therefore, it is avoided that when the reagent in the second chamber is extracted, the pressure above the reagent decreases to affect the smooth outflow of the reagent, so that the extraction of the reagent can be completed smoothly.

An embodiment of the present disclosure also provides a control method for a molecular diagnostic sample processing system, which may include the following steps: controlling the rotation sequence and rotation angle of the main valve body and the auxiliary valve body, so that the main valve body and the auxiliary valve body follow a predetermined sequence It communicates with the chamber on the cartridge, and realizes the reagent transfer between the main valve body and the auxiliary valve body and the connected chamber parts through the control valve stem, such as pushing reagents into the connected chambers or extracting reagents from the connected chambers. Every time the main and auxiliary valve bodies are rotated to communicate with the predetermined cabin, the origin of the main and auxiliary valve bodies can be firstly positioned, and the main valve body and the auxiliary valve body can be rotated back to the initial origin position, so that the main , The auxiliary valve body can precisely rotate a predetermined angle to communicate with the required compartment.

Description of drawings

In order to more clearly illustrate the technical solutions in the specific embodiments of the present disclosure or related technologies, the following will briefly introduce the drawings that need to be used in the specific embodiments or descriptions of related technologies. Obviously, the accompanying drawings in the following description are For some implementations of the present disclosure, those skilled in the art can also obtain other drawings based on these drawings without making creative efforts.

FIG. 1 is a schematic structural diagram of a cartridge provided by an embodiment of the present disclosure;

FIG. 2 is a schematic structural diagram of a molecular diagnostic sample processing system provided by an embodiment of the present disclosure;

FIG. 3 is a schematic structural diagram of a first operating module provided by an embodiment of the present disclosure;

Fig. 4 is a schematic structural diagram of the piercing mechanism of the first operating module provided by an embodiment of the present disclosure;

FIG. 5 is a schematic structural diagram of the main rotating assembly of the first operating module provided by an embodiment of the present disclosure;

Fig. 6 is a schematic structural diagram of a second operating module provided by an embodiment of the present disclosure.

Reference signs:

1-First operating module, 11-First lifting device, 12-Installation plate, 13-Block, 14-First piercing part, 15-Second piercing part, 16-Main rotation assembly, 161-First Driving device, 162-the second gear, 163-the first gear, 164-rotating connector, 165-locating pin, 166-origin positioning hole, 167-photoelectric switch, 17-pair rotating assembly;

2-second operating module, 21-second lifting device, 22-telescopic drive device, 23-valve stem connector;

3 - the third operating module;

4-cartridge, 41-main pipetting valve, 411-main valve body, 412-main valve stem, 413-positioning groove, 414-slot, 42-sub-pitting valve, 43-reagent tube, 44-section Two cabins.

Detailed ways

The technical solutions of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are some of the embodiments of the present disclosure, rather than all of them.

The components of the disclosed embodiments generally described and shown in the figures herein may be arranged and designed in a variety of different configurations. Accordingly, the following detailed description of the embodiments of the present disclosure provided in the accompanying drawings is not intended to limit the scope of the claimed disclosure, but merely represents selected embodiments of the present disclosure.

Based on the embodiments in the present disclosure, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present disclosure.

In the description of the present disclosure, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present disclosure and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, or in a specific orientation. construction and operation are therefore not to be construed as limitations on the present disclosure. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and should not be construed as indicating or implying relative importance.

In the description of the present disclosure, it should be noted that unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection. Connected, or integrally connected; it can be mechanically connected or electrically connected; it can be directly connected or indirectly connected through an intermediary, and it can be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present disclosure in specific situations.

The molecular diagnostic sample processing system and control method according to some embodiments of the present disclosure will be described below with reference to FIGS. 1 to 6 .

Next, some embodiments of the present disclosure will be described in detail with reference to the accompanying drawings.

The present disclosure provides a molecular diagnostic sample processing system, as shown in FIG. 1 and FIG. 2 , comprising a cartridge 4 and a second operating module 2 , a first operating module 1 and a third operating module 3 arranged side by side.

The cartridge 4 is a cartridge 4 in the related art. As shown in FIG. 1, a plurality of chambers configured to place various raw materials can be formed in the cartridge 4, and the plurality of chambers include sample chambers configured to place samples , configured as a plurality of first compartments, a plurality of second compartments 44, reconstitution compartments, and waste liquid compartments for placing various reagents, and a plurality of capillaries equipped with specific primers and probes are also arranged on the cartridge 4; The cartridge 4 is also provided with a main pipetting valve 41 and an auxiliary pipetting valve 42. By operating the main and auxiliary pipetting valves, the various raw materials in the cartridge 4 can be transferred between each compartment in a predetermined order to complete the pairing. Sample pretreatment, and filling the final sample processing solution into the capillary. The main pipetting valve 41 and the auxiliary pipetting valve 42 can be respectively rotatably connected to the cartridge 4. By rotating the valve bodies of the main and auxiliary pipetting valves, the main and auxiliary pipetting valves can be connected to different compartments respectively, specifically , the main pipetting valve 41 can communicate with the sample compartment, the waste liquid compartment, the reconstitution compartment, a plurality of first compartments and a plurality of second compartments 44, and the auxiliary pipetting valve 42 can communicate with the reconstitution compartment and a plurality of capillaries The main pipetting valve 41 and the auxiliary pipetting valve 42 are both piston valves, and the material transfer between the main and auxiliary pipetting valves and the connected compartments can be realized by pushing the valve body or pulling the valve stem outside the valve body.

When the cartridge 4 is placed in the pretreatment station, specifically, a moving mechanism can be provided to move the cartridge 4 to the pretreatment station; the first operating module 1 can drive the valve body of the main pipetting valve 41, that is, the main valve The valve body of the body 411 and the auxiliary pipetting valve 42, that is, the auxiliary valve body, rotates so that the main pipetting valve 41 and the auxiliary pipetting valve 42 can communicate with different compartments; the second operating module 2 can drive the main pipetting valve 41 The valve stem of the auxiliary pipetting valve 42, that is, the main valve stem 412, rises and falls, so that the main valve stem 412 performs piston movement in the main valve body 411, and the third operating module 3 can drive the valve stem of the auxiliary pipetting valve 42, that is, the auxiliary valve stem, to rise and fall, so that the auxiliary pipetting valve The valve stem moves as a piston in the auxiliary valve body, so as to realize the material transfer between the main and auxiliary pipetting valves and the connected compartments; furthermore, by setting the first operating module 1, the second operating module 2 and the third operating module 3 It can realize the fully automatic operation of the cartridge 4 to complete the sample pretreatment and capillary filling operation, which greatly improves the operation efficiency and operation accuracy compared with manual operation, and shortens the time required for molecular diagnosis.

Next, other embodiments of the present disclosure will be described in detail with reference to the accompanying drawings.

This embodiment is an improvement on the basis of the above embodiment. The technical content disclosed in the above embodiment will not be described repeatedly, and the content disclosed in the above embodiment also belongs to the content disclosed in this embodiment.

The cartridge 4 is provided with a reagent connecting tube 43, as shown in Figure 1, the reagent connecting tube 43 includes a plurality of reagent tubes, different reagents are respectively packaged in the multiple reagent tubes, and the multiple reagent tubes are placed on the card in one-to-one correspondence. Inside the plurality of first compartments of box 4. The bottom of the first chamber faces the interior of the chamber to form a third piercing part. When the reagent in the reagent tube needs to be used, downward pressure needs to be applied to the reagent connecting tube 43 so that the third piercing part can pierce the corresponding reagent. Tube.

In this embodiment, as shown in Figure 3 and Figure 4, the first operating module 1 may include a first lifting device 11 and a piercing mechanism, the driving end of the first lifting device 11 is provided with a mounting plate 12, the first lifting device 11 can drive the mounting plate 12 to rise and fall in the vertical direction, and the pretreatment station of the cartridge 4 is located below the mounting plate 12; surface, and the pressing block 13 is located directly above the reagent connecting tube 43; when the mounting plate 12 is lowered to a predetermined position, the pressing block 13 can be pressed tightly on the reagent connecting tube 43, so that the reagent tube is tightly pressed into the corresponding In the first compartment, the bottom of the reagent tube is punctured by the third piercing part, so that the reagent tube communicates with the corresponding first compartment, and the main pipetting valve 41 can extract reagent from the reagent tube.

In this embodiment, as shown in FIG. 4 , the piercing mechanism further includes a plurality of first piercing parts 14 corresponding to a plurality of first chambers; The part 14 is arranged on the pressing block 13, and a plurality of first piercing parts 14 are respectively located directly above the corresponding first compartment, so that when the installation plate 12 is lowered to a predetermined position, the pressing block 13 is tightly pressed against the reagent tube 43 When going up, not only the bottom of the reagent tube can be punctured by the third piercing part, but also a plurality of first piercing parts 14 can respectively pierce the sealing film on the upper end of the reagent tube in the corresponding first compartment, thereby avoiding the When the pipetting valve 41 extracts the reagent in the reagent tube, the pressure above the reagent in the reagent tube becomes smaller, which affects the smooth outflow of the reagent, thereby successfully completing the extraction of the reagent.

In this embodiment, the plurality of second compartments 44 of the cartridge 4 are covered with sealing films to seal the reagents in the second compartments 44 through the sealing films, in order to allow the reagents in the second compartments 44 to be The main pipetting valve 41 extracts, and as shown in Figure 4, the piercing mechanism also includes a plurality of second piercing parts 15, and the plurality of second piercing parts 15 corresponds to a plurality of second compartments 44 one by one, and the plurality of second piercing parts The piercing parts 15 are installed on the mounting plate 12 and are respectively located directly above the corresponding second compartments 44. When the mounting plate 12 is lowered to a predetermined position, a plurality of second piercing parts 15 can pierce the corresponding second compartments 44 The sealing film on the top, so that the reagents in the second chamber 44 can flow out smoothly.

Next, further embodiments of the present disclosure will be described in detail with reference to the accompanying drawings.

In this embodiment, as shown in FIG. 3 , the first operating module 1 further includes a main rotation assembly 16 and an auxiliary rotation assembly 17, and the main rotation assembly 16 and the auxiliary rotation assembly 17 are installed on the mounting plate 12 of the first lifting device 11 respectively. on, to be able to lift with the mounting plate 12. When the mounting plate 12 is lowered to a predetermined position, the main rotating assembly 16 can be connected with the valve body of the main pipetting valve 41, namely the main valve body 411, and the auxiliary rotating assembly 17 can be connected with the valve body of the auxiliary pipetting valve 42, namely the auxiliary valve body. are connected, and the main rotating assembly 16 is configured to drive the main valve body 411 to rotate, and the auxiliary rotating assembly 17 is configured to drive the auxiliary valve body to rotate, so that the main valve body 411 and the auxiliary valve body can be rotated by different angles to make the main The pipetting valve 41 and the auxiliary pipetting valve 42 communicate with different chambers respectively.

The structure of the main rotating assembly 16 and the auxiliary rotating assembly 17 is the same, and the structure of the main rotating assembly 16 and the auxiliary rotating assembly 17 will be described below taking the main rotating assembly 16 as an example.

In this embodiment, as shown in FIG. 5 , the main rotating assembly 16 includes a rotating connector 164 and a first driving device 161, and the rotating connecting member 164 is rotatably connected to the mounting plate 12 in the vertical direction, so that the rotating connecting member 164 Can rotate around its own axis. The first driving device 161 is fixedly mounted on the mounting plate 12 , and the driving end of the first driving device 161 is connected to the rotating connector 164 , so that the first driving device 161 can drive the rotating connecting member 164 to rotate.

The rotating connecting piece 164 is arranged coaxially with the main valve body 411. When the mounting plate 12 is lowered to a predetermined position, the lower end of the rotating connecting piece 164 can be connected with the main valve body 411, so that the rotating connecting piece 164 can drive the main valve body 411 synchronous rotation. The lower end of the rotating connector 164 is provided with a positioning pin 165, and the side wall of the main valve body 411 is provided with a positioning groove 413, and the positioning pin 165 can be inserted into the positioning groove 413, so that the rotating connecting member 164 is connected with the main valve body 411. The main valve body 411 can rotate synchronously with the rotating connecting member 164 .

As shown in Figures 1 and 5, the number of positioning grooves 413 can be multiple, and the multiple positioning grooves 413 are distributed along the circumferential direction of the main valve body 411 at intervals; the number of positioning pins 165 is also multiple, and the multiple positioning pins 165 Distributed at intervals along the circumferential direction of the rotating connector 164, and the plurality of positioning pins 165 correspond to the plurality of positioning grooves 413 one by one, and the plurality of positioning pins 165 can be respectively inserted into the corresponding plurality of positioning grooves 413, so that the rotating connecting member The connection between 164 and the main valve body 411 is more stable, and the main valve body 411 can rotate stably under the driving of the rotating connecting piece 164 .

In this embodiment, as shown in FIG. 5 , a first gear 163 is coaxially arranged on the rotating connector 164, and a second gear 162 is arranged on the driving end of the first driving device 161, and the second gear 162 is connected to the first gear. 163; the first driving device 161 can drive the second gear 162 to rotate, and then drive the first gear 163 and the rotating connecting member 164 to rotate.

In this embodiment, as shown in FIG. 5 , an origin positioning hole 166 is opened on the peripheral edge of the first gear 163 , and a photoelectric switch 167 is arranged on the mounting plate 12 close to the position of the first gear 163 , and the first gear The peripheral edge of 163 stretches into the sensing area of photoelectric switch 167; The main valve body 411 can be positioned at the origin through the origin positioning hole 166; then the main valve body 411 can be rotated at different angles to communicate with different compartments based on the origin position.

In the actual operation process, the origin of the main valve body is first positioned through the origin positioning hole 166, so that the main valve body is located at the initial origin position, and then the main valve body 411 is driven to rotate by a predetermined angle through the first driving device 161 to sequentially align with multiple Compartments are connected for material transfer.

The structure of the auxiliary rotating assembly 17 and the rotational driving of the auxiliary valve body by the auxiliary rotating assembly 17 are the same, and will not be repeated here.

In this embodiment, as shown in FIG. 2 , the second operating module 2 and the third operating module 3 can be respectively arranged on both sides of the first operating module 1 , and the second operating module 2 can be configured to drive the main valve stem 412 Lifting, so that the main valve rod 412 makes a piston movement in the main valve body 411, so that the main pipetting valve 41 can extract the reagent from the compartment connected with it or push the reagent in the main pipetting valve 41 to the compartment connected with it In the room, material transfer between the main pipetting valve 41 and the connected compartment is realized. The third operating module 3 can be configured to drive the auxiliary valve stem to move up and down, so that the auxiliary valve stem makes a piston movement in the auxiliary valve body, so as to realize the reagent transfer between the auxiliary pipetting valve 42 and the connected chamber.

The second operating module 2 has the same structure as the third operating module 3 , and the structures of the second operating module 2 and the third operating module 3 will be described below taking the second operating module 2 as an example.

In this embodiment, as shown in FIG. 6 , the second operating module 2 includes a valve stem connector 23, a second lifting device 21 and a telescopic driving device 22, the telescopic driving assembly is installed on the driving end of the second lifting device 21, and the valve The rod connector 23 is installed on the driving end of the telescopic drive device 22, so that the second lifting device 21 can drive the valve rod connector 23 to move vertically, and the telescopic drive device 22 can drive the valve rod connector 23 to move horizontally.

In this embodiment, the valve stem connector 23 is in the shape of a fork, and a slot 414 is provided on the side wall of the main valve stem 412. When the second lifting device 21 drives the valve stem to move vertically to a predetermined height, the valve stem The connecting piece 23 is at the same height as the slot 414, and then the valve stem connecting piece 23 is driven to move horizontally towards the main valve stem 412 by the telescopic drive device 22, so that the main valve stem 412 can extend to the two forks of the valve stem connecting piece 23 Between the arms, and the two fork arms of the valve stem connecting piece 23 are inserted into the slot 414 of the main valve stem 412 , so that the valve stem connecting piece 23 is connected with the main valve stem 412 .

After the valve stem connector 23 and the main valve stem 412 are plugged together, the second lifting device 21 drives the valve stem connector 23 to move up and down in the vertical direction, so that the main valve stem 412 can be lifted up and down along the vertical direction. Therefore, the main valve rod 412 makes a piston movement in the main valve body 411 to realize reagent transfer between the main pipetting valve 41 and the connected chamber.

The structure of the third operating module 3 is the same as that of the second operating module 2 , which will not be repeated here.

Next, some other embodiments of the present disclosure will be described in detail with reference to the accompanying drawings.

This embodiment provides a control method for the molecular diagnosis sample processing system in the above embodiment, so that the main pipetting valve and the auxiliary pipetting valve can transfer the reagents in the cartridge according to a predetermined order, so that the sample and Various reagents react according to a predetermined sequence, and the reacted sample processing solution is filled in the capillary.

In an embodiment of the present disclosure, the control method of the molecular diagnostic sample processing system, for example, includes the following steps:

Firstly, the sealed reagent cavity on the cartridge is punctured by the puncturing mechanism, specifically, the reagent tube is pressed down into the first compartment through the pressing block, so as to pass through the third punctured part of the bottom wall of the first compartment Puncture the lower ends of the multiple reagent tubes of the reagent manifold, simultaneously pierce the sealing films on the upper ends of the multiple reagent tubes through the first piercing part, and pierce the sealing films on the multiple second compartments through the second piercing part.

Then control the predetermined angle of rotation of the main valve body and/or the auxiliary valve body, so that the main valve body and the auxiliary valve body communicate with the compartment on the cartridge according to a predetermined order, and realize the communication between the main valve body and the auxiliary valve body by controlling the valve stem. Reagent transfer of compartments, such as pushing reagents into connected compartments or extracting reagents from connected compartments.

When the main valve body or the auxiliary valve body is turned to communicate with the predetermined cabin for the first time, the origin of the main valve body or the auxiliary valve body is firstly positioned, and the main valve body and the auxiliary valve body are turned back to the original origin position, so that The main and auxiliary valve bodies can be precisely rotated at predetermined angles to communicate with required compartments.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present disclosure, not to limit them; although the present disclosure has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present disclosure. scope.

Industrial Applicability

In summary, the present disclosure provides a molecular diagnostic sample processing system and control method. The molecular diagnosis sample processing system and control method can smoothly transfer raw materials in the cartridge, thereby smoothly completing the extraction of reagents. It can be understood that the molecular diagnostic sample processing system and control method of the present disclosure are reproducible and can be used in various industrial applications. For example, the molecular diagnosis sample processing system and control method of the present disclosure can be used in the technical field of molecular diagnosis.

Claims

A molecular diagnostic sample processing system, characterized in that it includes a cartridge and a first operating module;

The cartridge is formed with a plurality of second compartments, and a main pipetting valve is movably connected to the cartridge;

The multiple second compartments are configured to store reagents, and the tops of the multiple second compartments storing reagents are respectively covered with sealing films;

The first operating module can be connected with the main valve body of the main pipetting valve and drive the main valve body to move, so that the main valve body communicates with one of the plurality of second chambers respectively ;

The first operating module includes a first lifting device and a piercing mechanism, the piercing mechanism is installed on the driving end of the first lifting device, and the first lifting device can drive the piercing mechanism toward the card The cartridge is moved to cause the piercing mechanism to pierce the seal on a plurality of the second compartments.
The molecular diagnostic sample processing system according to claim 1, wherein the piercing mechanism comprises a plurality of second piercing parts;

A plurality of the second piercing portions correspond to the plurality of the second chambers one by one, and the plurality of second piercing portions are configured to pierce the sealing film of the corresponding second chambers.
The molecular diagnostic sample processing system according to claim 1 or 2, wherein the cartridge is provided with a plurality of first compartments and reagent tubes, and the reagent tubes of the reagent tubes correspond to one another placed in a plurality of said first compartments;

The piercing mechanism also includes a plurality of first piercing parts, and the multiple first piercing parts correspond to the plurality of reagent tubes one by one;

The plurality of first piercing parts are configured to pierce the sealing film on the corresponding reagent tube.
The molecular diagnostic sample processing system according to claim 3, wherein the driving end of the first lifting device is further provided with a pressing block;

The first lifting device can drive the pressing block to press on the reagent tube, so as to press a plurality of the reagent tubes into the corresponding first compartment;

The bottoms of the plurality of first compartments are respectively provided with third piercing parts, and the third piercing parts are configured to pierce the bottoms of the corresponding reagent tubes.
The molecular diagnostic sample processing system according to claim 3, characterized in that, a plurality of the first piercing parts are arranged on the pressing block, and the multiple first piercing parts are respectively located in the corresponding Directly above the first cabin.
The molecular diagnostic sample processing system according to claim 4 or 5, characterized in that, a plurality of the first piercing parts are arranged at intervals on the lower surface of the pressing block.
The molecular diagnostic sample processing system according to any one of claims 3-6, wherein the driving end of the first lifting device is further equipped with a main rotation assembly and an auxiliary rotation assembly;

The main valve body is rotatably connected to the cartridge, and an auxiliary pipetting valve is also rotatably arranged on the cartridge;

The first lifting device can drive the main rotating assembly and the auxiliary rotating assembly to move, so that the main rotating assembly is connected to the main valve body of the main pipetting valve, and the auxiliary rotating assembly is connected to the main valve body of the main pipetting valve. The auxiliary valve body of the auxiliary pipetting valve is connected;

The main rotating assembly is configured to drive the main valve body to rotate, so that the main valve body can be connected with the sample compartment, waste liquid compartment, reconstitution compartment, multiple first compartments or multiple compartments of the cartridge. The two second chambers are connected; the auxiliary rotation assembly is configured to drive the auxiliary valve body to rotate, so that the auxiliary valve body can be connected with the reconstitution chamber or a plurality of capillary tubes placed on the cartridge connected.
The molecular diagnostic sample processing system according to claim 7, characterized in that, both the main rotating assembly and the auxiliary rotating assembly include a rotating connection and a first driving device;

The rotating connecting piece is arranged in a rotatable manner, and the first driving device can drive the rotating connecting piece to rotate;

The rotating connector is provided with positioning pins, and positioning grooves are provided on the main valve body and the auxiliary valve body;

The locating pin can be plugged into the corresponding locating groove of the main valve body or the auxiliary valve body.
The molecular diagnostic sample processing system according to claim 8, characterized in that, the driving end of the first lifting device is provided with a mounting plate, and the rotating connecting member is rotatably connected to the mounting plate in the vertical direction, so as to The rotating connector can rotate around its own axis; the first driving device is fixedly installed on the mounting plate, and the driving end of the first driving device is connected with the rotating connecting member to drive the Rotate the connector to rotate.
The sample processing system for molecular diagnostics according to claim 9, wherein the rotating connecting member is arranged coaxially with the main valve body, and the rotating connecting member is configured so that when the mounting plate is lowered to a predetermined position , the lower end of the rotating connecting piece can be connected with the main valve body, so that the rotating connecting piece drives the main valve body to rotate synchronously.
The molecular diagnostic sample processing system according to any one of claims 8-10, wherein a plurality of positioning pins are distributed at intervals along the circumference of the rotating connector, and the plurality of positioning pins are connected to the plurality of positioning pins. There is a one-to-one correspondence between the positioning slots.
The molecular diagnostic sample processing system according to any one of claims 8-11, characterized in that, a first gear is coaxially arranged on the rotating connector, and a second gear is arranged at the driving end of the first driving device. gear;

The first driving device can drive the second gear to rotate, and the second gear is meshed with the first gear.
The sample processing system for molecular diagnosis according to claim 12, wherein the first gear is provided with an origin positioning hole, one side of the first gear in the radial direction is provided with a photoelectric switch, and the first The gear can be rotated until the origin positioning hole is opposite to the photoelectric switch, so as to locate the origin of the main valve body or the auxiliary valve body.
The molecular diagnostic sample processing system according to any one of claims 7-13, further comprising a second operating module and a third operating module;

The second operating module and the third operating module are respectively arranged on both sides of the first operating module;

Both the main pipetting valve and the auxiliary pipetting valve are piston valves, the second operating module is configured to drive the main valve rod of the main pipetting valve to perform piston movement, and the third operating module is configured to To drive the auxiliary valve stem of the auxiliary pipetting valve to do piston movement.
The molecular diagnostic sample processing system according to claim 14, wherein the second operating module and the third operating module both include a second lifting device, a telescopic driving device and a valve stem connector;

The valve stem connecting piece is installed on the driving end of the second lifting device through the telescopic driving device;

The telescoping driving device is configured to drive the valve stem connecting piece to protrude toward the corresponding main valve stem or the auxiliary valve stem, so that the valve stem connecting piece is aligned with the corresponding main valve stem and the corresponding main valve stem. The auxiliary valve stem is connected;

The second elevating device is configured to drive the valve rod connecting piece to elevate, so as to drive the corresponding main valve rod or the auxiliary valve rod to elevate.
The molecular diagnostic sample processing system according to claim 15, wherein the valve stem connecting piece is fork-shaped;

Both the main valve stem and the auxiliary valve stem are provided with slots, and the valve stem connectors can be plugged into the slots.
A method for controlling a molecular diagnostic sample processing system, comprising the following steps:

Perform puncture pretreatment on the sealed reagent compartment of the cartridge;

Control the rotation of the main valve body and/or the auxiliary valve body to a predetermined angle, so that the main valve body and/or the auxiliary valve body are connected with the compartments in the cartridge according to a predetermined order, and the main and auxiliary valve bodies are realized by controlling the movement of the valve stem. Reagent transfer with compartment pieces in communication.
The control method of the molecular diagnostic sample processing system according to claim 17, further comprising the following steps:

When the main valve body or the auxiliary valve body is turned to communicate with the predetermined compartment for the first time, the origin of the main valve body or the auxiliary valve body is positioned.