CN218620280U - Connecting pipe type nucleic acid reaction consumable opening and closing cover mechanism - Google Patents

Abstract

The scheme discloses a connecting pipe type nucleic acid reaction consumable material cover opening and closing mechanism which comprises a first operation layer, a second operation layer and a third operation layer which are sequentially arranged from top to bottom; and a power mechanism arranged between the two; the power mechanism can drive the second operation layer to move up and down along the vertical direction; a plurality of elastic elements are arranged between the second operation layer and the third operation layer; the limiting part is used for limiting the limit position of the third operation layer; when the power mechanism drives the second operation layer to move downwards, the third operation layer also moves downwards, when the third operation layer reaches the limit position, the second operation layer continues to move downwards to enable the elastic element to be compressed, the elastic element in the compressed state is in close contact with the third operation layer, and therefore the compression applied to the consumable body can be realized by utilizing the compressed state of the spring, and the fact that the cover is not pulled up in the process of opening the cover by additionally designing a fixing structure matched with the cover is not needed.

Description

Connecting pipe type nucleic acid reaction consumable opening and closing cover mechanism

Technical Field

The utility model relates to the field of automatic realization of medical instrument, in particular to a connecting tube type nucleic acid reaction consumable switch cover mechanism.

Background

Nucleic acids are carriers of genetic information in organisms, and isolation, purification, and detection of nucleic acids are required for studies of nucleic acid sequences and structures, and for studies of gene functions and expression.

Among them, nucleic acid amplification such as Polymerase Chain Reaction (PCR) is increasingly important in the fields of genetic engineering, molecular biology, food safety, environmental monitoring, medicine for analysis and diagnosis, and the like. Because of their high sensitivity and specificity, many biological researchers have used PCR and other forms of DNA amplification in their nucleic acid analysis efforts. For performing PCR, a biological sample containing nucleic acids is first treated to obtain the nucleic acids in purified or unpurified form, which is then formulated into a reaction material and loaded into a reactor, for example in the form of a tube or well plate. Thereafter, the reactor is subjected to a heat treatment, e.g., thermal cycling, in a PCR module (PCRM) so that the PCR thermal cycling amplification reaction can be completed.

However, the above amplification reaction can be generally divided into two main processes: 1) A nucleic acid extraction reaction process and 2) an amplification reaction process; at present, with the increasing improvement of the detection quantity and the requirements of consistency and reliability of detection results, more and more devices are designed to be capable of automatically operating nucleic acid extraction reaction and nucleic acid amplification reaction, whereas the reaction vessels involved in the two respective processes are generally designed as disposable consumables or consumable components, for example, tip consumables, stirring sleeve consumables, a cracking hole site, a washing hole site, an elution hole site and the like can be designed into a combined disposable consumable component in the extraction process, in order to ensure that the consumable can be used efficiently and at high speed, the consumable may be prepackaged with reagents in different wells, and then the disposable consumable is fitted with a lid, therefore, the packaging of the combined consumable is realized, in addition, similarly, a scene that a plurality of target gene fragments need to be detected simultaneously can be involved in the nucleic acid amplification reaction, a premixing hole site is also needed to premix a sample to be detected, therefore, it is also necessary to pre-package the corresponding test requirement reagents at different hole sites, and therefore, the test requirement reagents are often designed into a combined porous consumable structure, and the pre-package scheme for the amplification reagents is implemented by matching with the cover body, however, the tight connection between the container body and the cover body in the two processes is relatively large in order to ensure the sealing effect, the traditional method is that when in use, an operator opens the cover and places the consumable after opening the cover at the corresponding hole site, then corresponding reaction or experiment operation is performed, and after completion, the experiment operator closes the corresponding container and container cover, however, the opening or closing of the cover has some relatively serious risks, 1) because the cover and the body are connected relatively tightly to open or close the cover, reagent spills may exist, and operation difficulty of operators is doubled or geometric grade is increased due to operation of a large number of samples; 2) There is a risk of infection or contamination of the cover by the operator manually actuating the switch cover itself.

Therefore, an automatic cover opening and closing mechanism which can adapt to the situation that the cover body is tightly connected with the body under the nucleic acid reaction scene needs to be designed in a matching manner, so that the extraction and amplification reaction can be completed in a fully automatic and manual-free manner in the process, and the cover closing consistency and the reliability effect are also ensured.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a: to the problem that above-mentioned exists, the utility model provides a connecting tube type nucleic acid reaction consumptive material switch lid mechanism.

The utility model discloses a realize through following scheme:

a connecting pipe type nucleic acid reaction consumable opening and closing cover mechanism comprises a first operation layer, a second operation layer and a third operation layer which are sequentially arranged from top to bottom; and a power mechanism arranged between the two; the power mechanism can drive the second operation layer to move up and down along the vertical direction; a plurality of elastic elements are arranged between the second operation layer and the third operation layer; the switch cover mechanism also comprises a limiting part for limiting the limit position of the third operation layer; when the power mechanism drives the second operation layer to move downwards, the third operation layer also moves downwards, when the third operation layer reaches the limit position, the second operation layer continues to move downwards to enable the elastic element to be compressed, and the elastic element in the compressed state is in close contact with the third operation layer.

Through the structure, this scheme is with first operation layer, second operation layer and third operation layer organic make up, make the second operation layer can drive third operation layer under power unit's drive, when third operation layer moves extreme position through its spacing portion, the second operation layer continues to move relatively with the third operation under power unit's drive, make the elastic component compression, a decurrent thrust can be applyed to third operation layer to the elastic component of compressed, third operation layer can cooperate with the consumptive material main part in making this scheme, in-process opening the consumptive material lid, to compressing holding power with third operation layer contact complex consumptive material main part, make it need not extra part to fix the consumptive material main part and can realize opening and closing the operation to the consumptive material lid.

Preferably, the power mechanism can drive N running units (where N is an integer not less than 2) to move synchronously with the second working floor, and the N running units simultaneously apply driving force to the second working floor at multiple points to drive the second working floor to move up and down along the vertical direction.

Based on the structure, the plurality of operation units matched with the power mechanism are arranged in the second operation layer, so that when the power mechanism drives the second operation layer, the acting force can be evenly distributed through the operation units arranged in a multipoint distribution mode, and the second operation layer can be stably driven by the power mechanism to move upwards or downwards.

Preferably, the operation unit is a screw rod unit, and the screw rod unit can be driven by the power mechanism through a belt wheel and/or a gear set.

Based on the structure, the second operation layer can move upwards or downwards under the driving of the power mechanism through the screw rod unit; simultaneously, the driving of the second operation layer is realized through the matching of the belt wheel and the screw rod unit or the matching of the gear set and the screw rod unit.

Preferably, the screw unit is disposed between the first working floor and the second working floor.

Preferably, a plurality of first guide rods are arranged between the first operation layer and the third operation layer, and the third operation layer can move relative to the first operation layer along the length direction of the first guide rods along with the second operation layer under the driving of the power mechanism.

According to the above structure, the first guide rod can provide a guide function for the first operation layer and the third operation layer, and the third operation layer and the second operation layer are connected into a whole by the elastic element arranged between the third operation layer and the second operation layer, so that the third operation layer can move upwards or downwards along with the action of the second operation layer.

Preferably, the upper end of the first guide rod is provided with a limiting part of a third working layer limit position; the limiting part is positioned above the first operation layer; the power mechanism is directly or indirectly connected with the second operation layer, and the power mechanism body also moves up and down along with the second operation layer in the process that the power mechanism drives the second operation layer to move up and down along the vertical direction.

Based on the structure, one feasible operation mode in the scheme is to arrange the limiting part above the first guide rod, and at the moment, when the power mechanism drives the second operation layer to move, the power mechanism body also moves up and down along with the second operation layer.

Preferably, the limiting part is arranged at the lower end of the first guide rod; the limiting part is positioned below the third operation layer; the power mechanism is directly or indirectly connected with the first operation layer, so that the power mechanism body is fixed when the power mechanism drives the second operation layer to move up and down along the vertical direction.

Based on the structure, one feasible operation mode in the scheme is to arrange the limiting part below the first guide rod, and at the moment, the power mechanism can keep still when driving the second operation layer to move.

Preferably, a plurality of second guide rods are vertically arranged between the second operation layer and the third operation layer; the upper end of the second guide rod penetrates through the second operation layer, and the lower end of the second guide rod is connected with the third operation layer; the elastic element is sleeved on the second guide rod.

Based on above-mentioned structure, the second guide bar provides the direction for spring deformation, makes the deformation of spring in controllable range, can guarantee that all spring dynamics are the same basically, makes the second operation layer balanced to the pressure on third operation layer.

To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:

1. the limiting part is used for limiting the limiting position of the third operation layer; when the lifting motor drives the second operation layer to move downwards, the third operation layer also moves downwards, when the third operation layer reaches the limit position, the second operation layer continues to move downwards so that the elastic element is compressed to apply a downward thrust to the third operation layer, the consumable body is compressed by the aid of the compressed state of the spring, even if the compressed state of the spring of the reverse rotation motor can be continuously maintained, and accordingly continuous force for pressing the consumable body downwards is generated in the cover opening process.

2. Pass through in this scheme elevator motor can drive N with the run-in synchronism unit operation that second operation layer is direct or indirect to it is right simultaneously to realize can the multiple spot the second operation layer applys the overdraft, so guaranteed that switch lid unit can more steadily apply switch lid effort uniformly, guaranteed switch lid's high efficiency and reliability operation.

3. Different two kinds of switch cover structural design can be realized through the different position setting of spacing portion in this scheme, and the structural design of the higher reliability of small volume can be realized to the different mounted position of cooperation elevator motor, and the setting of cooperation a plurality of first guide bars has guaranteed that elevator motor can accomplish the switch cover operation along setting for the orbit more reliably and accurately.

Drawings

FIG. 1 is a schematic diagram of a consumable material for nucleic acid reaction according to the present invention;

FIG. 2 is a schematic structural view of a cover opening and closing mechanism and a carrier platform according to the present invention;

FIG. 3 is a schematic view of a carrier stage according to the present invention;

fig. 4 is a detailed schematic view of a switch cover structure provided in embodiment 1 of the present invention;

fig. 5 is a detailed schematic view of another switch cover structure provided in embodiment 2 of the present invention;

FIG. 6 is a schematic structural diagram of a second operation layer of the present invention;

fig. 7 is a schematic side view of the second working layer of the present invention;

FIG. 8 is a schematic structural diagram of a third operation layer in the present invention;

fig. 9-16 are schematic diagrams of a cover opening and closing mechanism provided in the present invention for performing a cover opening process;

fig. 17-20 are schematic diagrams illustrating a lid closing process executed by the lid opening and closing mechanism provided in the present invention.

Fig. 21 is a diagram showing an example of the operation unit in embodiment 1 of this embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, as presented in the figures, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

Example 1

As shown in FIG. 4, the present embodiment provides a consumable article for nucleic acid reaction opening and closing mechanism, which comprises a first operation layer 14, a second operation layer 18 and a third operation layer 19, which are arranged from top to bottom; a plurality of first guide rods 20 are vertically arranged below the first operation layer 14, the upper ends of the first guide rods 20 are connected with the first operation layer 14, and the lower ends of the first guide rods 20 penetrate through the third operation layer 19, so that the third operation layer 19 can move up and down along the first guide rods 20.

The upper end of the first guide rod 20 is provided with a first guide rod limit position limiting part 201; the first guide bar limit position stopper 201 is located above the first working layer 14.

A plurality of second guide rods 27 are vertically arranged between the second operation layer 18 and the third operation layer 19; the upper end of the second guide rod 27 penetrates through the second operation layer 18, the lower end of the second guide rod 27 is connected with the third operation layer 19, the upper end of the second guide rod 27 is provided with a second guide rod upper limiting block, and the second guide rod upper limiting block is positioned above the second operation layer 18.

The second guide rod 27 is sleeved with an elastic component 21, and the elastic component 21 is positioned between the second operation layer 18 and the third operation layer 19; the first working floor 14 is directly or indirectly connected to the frame structure of the whole device, the lifting motor 15 is directly or indirectly connected to the second working floor 18, and the lifting motor 15 is used for driving the second working floor 18 to move up and down along the first guide rod 20.

In the embodiment, the lifting motor 15 can drive N synchronous operation units directly or indirectly connected with the second operation layer 18 to operate (where N is an integer not less than 2), so that the N operation units can simultaneously apply driving force for driving the second operation layer 18 to move up and down along the vertical direction at multiple points, wherein the N operation units can be in a structure that 4 pulleys 281 are transmitted to four connected screw rods 28.

Referring to fig. 21, the present embodiment explains the N operation units, where the N operation units may include a first lead screw 41, a second lead screw 42, a third lead screw 43, and a fourth lead screw 44; a first belt wheel 31, a second belt wheel 32, a third belt wheel 33, a fourth belt wheel 34 and a transmission belt 45 which are matched with the first belt wheel, the second belt wheel, the third belt wheel and the fourth belt wheel; the first screw rod 41 is sleeved in the first belt wheel 31, and the second screw rod 42 is sleeved in the second belt wheel 32; the third screw 43 is sleeved in the third belt pulley 33; the fourth screw 44 is sleeved in the fourth pulley 34;

the first pulley 31, the second pulley 32, the third pulley 33, and the fourth pulley 34 are uniformly arranged on the second working floor.

The lifting motor is fixedly arranged on the second operation layer, and a through groove for the penetration of the lifting motor is arranged in the first operation layer; an output belt wheel 46 is arranged at the output end of the lifting motor, and the first belt wheel 31, the second belt wheel 32, the third belt wheel 33, the fourth belt wheel 34 and the output belt wheel are connected into a whole by the conveyor belt 45.

And an auxiliary wheel 47 for tensioning and guiding the conveying belt is further arranged on the second working surface, the conveying belt is guided by the auxiliary wheel 47, the contact area between the conveying belt and each belt wheel is larger by guiding, and the power transmission is more stable.

The fitting relationship between the first lead screw 41 and the first belt pulley 31 is the same as that between other lead screw belt pulleys, and here, the fitting relationship between the first lead screw 41 and the first belt pulley 31 is taken as an explanation; the upper end of the first screw rod 41 is fixedly connected with the first operation layer, the lower end of the first screw rod 41 penetrates through the second operation surface, the first belt wheel 31 is in threaded connection with the first screw rod 41, the first belt wheel 31 is driven to rotate through the rotation of the lifting motor, and therefore the first belt wheel 31 runs along the length direction of the first screw rod 41, and the second operation layer is driven to integrally move up and down.

Similarly, the second pulley 32 acts with the second lead screw 42; the third belt pulley 33 and the third screw 43; the fourth belt pulley 34 and the fourth screw rod 44 act to further achieve the effect that one motor drives four screw rods to synchronously operate to drive the second working layer 18 to be pressed down basically uniformly and without deflection, and of course, the structure that the motor drives 4 or other number of screw rods to synchronously press down, which is implemented by the belt pulley transmission mechanism in the above description, can be implemented by the transmission of engaged gear pairs, and is not limited here.

Example 2

As shown in FIG. 5, the present embodiment provides another consumable part for nucleic acid reaction mechanism for opening and closing a cover, comprising a first operation layer 14, a second operation layer 18 and a third operation layer 19, which are sequentially arranged from top to bottom; a plurality of first guide rods 20 are vertically arranged below the first operation layer 14, the upper ends of the first guide rods 20 are connected with the first operation layer 14, and the lower ends of the first guide rods 20 penetrate through the second operation layer 18 and the third operation layer 19, so that the second operation layer 18 and the third operation layer 19 can move up and down along the first guide rods 20.

The lower end of the first guide rod 20 is provided with a first guide rod lower limit block (similar to the limit position limit part 201 in fig. 4); the lower limiting block of the first guide rod is positioned below the third operation layer 19; a plurality of second guide rods 27 are vertically arranged between the second operation layer 18 and the third operation layer 19; the upper end of the second guide bar 27 extends through the second working floor 18 and the lower end of the second guide bar 27 is connected to the third working floor 19.

The upper end of the second guide rod 27 is provided with a second guide rod upper limiting block which is positioned above the second operation layer 18; the second guide rod 27 is sleeved with an elastic component 21, and the elastic component 21 is positioned between the second operation layer 18 and the third operation layer 19; the first operation layer 14 is connected with the second operation layer 18 through a lifting device, and a lifting motor is used for driving the second operation layer 18 to move up and down along a first guide rod 20; the lifting motor can drive the cylinder, the oil cylinder, the screw rod mechanism and the like.

In this embodiment, the lifting motor 15 is fixedly disposed above or below the first operation layer 14, the upper end of the screw rod 16 is connected to the screw rod motor 15, the screw rod connection seat 17 is disposed above the second operation layer 18, and the lower end of the screw rod 16 penetrates through the second operation layer 18 and the screw rod connection seat 17 and is in threaded connection with the screw rod connection seat 17, although a scene that the lifting motor drives the screw rod to press down in a single point is illustrated here, in practical use, a pulley screw rod transmission driven by the lifting motor may be configured to be similar to the design in fig. 4, where the first guide rod 20 may be replaced by four synchronous rotation portions driven to rotate, so as to implement a more balanced and non-inclined pressing operation.

The difference between the embodiment 1 and the embodiment 2 is that the lifting motor 15 in the structure of the embodiment 1 can drive N synchronous operation units directly or indirectly connected with the second operation layer 18 to operate (where N is an integer not less than 2), the lifting motor 15 is directly or indirectly connected with the second operation layer 18, so that the lifting motor 15 moves up and down along with the second operation layer 18 when the lifting motor 15 drives the second operation layer 18 to move up and down along the vertical direction, and the upper end of the first guide rod 20 in the structure is provided with a limiting part 201 of the limiting position of the third operation layer 19; the limiting part 201 is positioned above the first operation layer; in the embodiment 2, the lifting motor 15 is directly or indirectly connected with the first operation layer 14, so that the lifting motor 15 is fixed when the lifting motor 15 drives the second operation layer 18 to move up and down along the vertical direction, and in the structure, the lower end of the first guide rod 20 is provided with a limiting part 201 at the limit position of the third operation layer; the limiting portion 201 is located below the third operation layer, so that the effect of freely arranging different unit structure relationships can be achieved through two different structural designs of the embodiment, and the reliability of the design can be guaranteed, and the low-cost performance of the design can also be guaranteed.

Example 3

This example takes the consumable nucleic acid reaction material shown in FIG. 1 as an example to illustrate the mechanism for opening and closing the lid of the consumable nucleic acid reaction material in this embodiment.

As shown in FIG. 1, the consumable structure for combined extraction reaction comprises a consumable body 1 and a consumable cover 2; the consumable main body 1 comprises a connecting plate 3 and a plurality of pipe bodies 4 inserted into the connecting plate 3, wherein the pipe bodies 4 are provided with pipe orifices 5, the pipe orifices 5 of all the pipe bodies 4 are positioned on the same plane, and the width of the connecting plate 3 is larger than the diameter of the pipe orifices 5 so as to form a first cornice 6 for fixing the pipe bodies 4 when the cover is opened on the outer side wall of the pipe bodies 4; the consumable cover 2 comprises a plurality of pipe covers 7 which correspond to the pipe bodies 4 one by one and are used for blocking the pipe orifices 5, the pipe covers are connected to the same side of the pipe cover plate 8, and second cornices 9 which are taken as stress points when the pipe covers 7 are pulled out of the pipe bodies 4 are arranged on the edges of the pipe covers 7; when the body 4 was fitted to the lid 7 lid, be formed with the clearance between second cornice 9 and the first cornice 6, be convenient for the utility model provides a device inserts from this clearance, and then realizes the operation of uncapping. The consumable in the figure comprises a plurality of hole sites for receiving different reagents or other consumables, which can respectively bear a Tip consumable hole site, a lysis hole site, a washing hole site, an elution hole site, a stirring sleeve hole site and another Tip consumable hole site, fig. 1 shows a state diagram (left diagram) of the cover separated from the body, and a state diagram of the cover buckled and the body is shown in the right diagram, and a Tip head and a stirring sleeve are arranged in the hole sites, of course, the combined consumable in the figure can be a PCR amplification reaction consumable including a PCR reaction liquid premixing hole site and an aliquot multi-detection hole site for multi-target gene fragment detection, and the like, and the figure is not limited.

In the scheme, the X-axis direction, the Y-axis direction and the Z-axis direction are directions shown in fig. 2, the consumable material clamping grooves 12b, the consumable material cover clamping grooves 22 and the consumable material main body fixing grooves 24 extend along the Y axis and are arranged in parallel at equal intervals along the X-axis direction, and the inlet ends of the cover opening and closing mechanisms 11 are the front end opening positions of the consumable material cover clamping grooves 22 and the consumable material main body fixing grooves 24; and the consumable loading table 10 can be driven to move along the Y-axis direction, and the cover opening and closing operation of the nucleic acid reaction consumable is realized by matching with the up-and-down movement of the cover opening and closing mechanism 11. FIG. 3 shows a specific structure of the consumable material loading platform, wherein the sample tube receiving hole 12a is disposed at an upstream position along the Y-axis direction in the nucleic acid reaction consumable material card slot 12b along the lid opening movement direction, and it comprises a locking structure 13 for limiting the lid opening/closing mechanism 11, so as to mechanically ensure the reliable fit between the lid opening/closing mechanism 11 and the consumable material loading platform 10 during the lid opening/closing operation.

Meanwhile, the embodiment also provides a consumable loading platform connecting piece matched with the combined extraction reaction consumable structure; the consumable loading platform connecting piece is arranged at the bottom position of the third operation layer and is used for detachably connecting the third operation layer 19 and the consumable loading platform 10 together.

The connecting piece of the consumable loading platform is a fixing strip 25 arranged at the bottom of the third operation layer 19, a clamping interface 26 is arranged on the fixing strip 25, the clamping interface 26 is used for enabling a fixing bolt 13 arranged on the side wall of the consumable loading platform 10 to be embedded into the clamping interface 26 from the horizontal direction, and the third operation layer 19 and the consumable loading platform 10 are limited to be separated vertically. The consumable body fixing mechanism is a plurality of consumable body fixing grooves 24 arranged on the third operation layer 19, and the consumable body fixing grooves 24 are arranged in a row at equal intervals in parallel and penetrate through the third operation layer 19 in the vertical direction.

24 front end openings in consumptive material main part fixed slot make things convenient for 4 upper ends of body on the consumptive material main part 1 to get into the fixed slot from fixed slot front end opening in, when 4 upper ends of body on the consumptive material main part 1 are whole to be imbedded in consumptive material main part fixed slot 24, the connecting plate 3 of consumptive material main part 1 can be pushed down to 24 lower edges in consumptive material main part fixed slot, is uncapping the fixed consumptive material main part 1 of in-process. The length of the consumable body fixing groove is more than or equal to that of the consumable body 1. The extending direction of the consumable body fixing groove 24 is consistent with the extending direction of the consumable cover clamping groove 22; the number of consumable body fixing slots 24 corresponds to the number of consumable cover slots 22 and is aligned with the consumable cover slots 22.

The uncapping step and the uncapping method realized by the scheme are described in detail with reference to fig. 9-16:

before the experiment is started, the user places the reaction consumables in a closed state into the consumable card slot 12b of the consumable loading table 10. Firstly, the consumable material loading table 10 is driven by the moving mechanism to move to a position corresponding to the inlet end of the cover opening and closing mechanism 11 along the Y-axis direction, as shown in fig. 9; in the utility model, the direction of the X axis, the direction of the Y axis and the direction of the Z axis are the directions shown in fig. 2, the consumable material clamping groove 12b, the consumable material cover clamping groove 22 and the consumable material main body fixing groove 24 all extend along the Y axis and are arranged in parallel along the X axis at equal intervals, and the inlet end of the switch cover mechanism 11 is the opening position at the front end of the consumable material cover clamping groove 22 and the consumable material main body fixing groove 24;

next, the lifting motor 15 pushes the second operation layer 18 and the third operation layer 19 to move downwards along the Z-axis (the cover opening and closing mechanism in fig. 4 is taken as an example here), so that the third operation layer 19 reaches the lower limit position (the lower limit position is reached due to the limit function of the limit part 201 on the upper part of the first operation layer 14), at this time, the lower edge of the consumable body fixing groove 24 on the third operation layer 19 corresponds to the connection plate 3 of the consumable body 1, that is, the lower edge of the consumable body fixing groove 24 is substantially flush with the upper surface of the connection plate 3, as shown in fig. 10;

next, the lifting motor 15 continues to push the second operation layer 18 to move downward along the Z-axis, and compresses the four springs between the second operation layer 18 and the third operation layer 19, so that the highest point of the uncovering protrusion 23a at the inlet end of the consumable cover clamping groove 22 on the second operation layer 18 is slightly higher than the tube cover plate 8 of the consumable cover 2, as shown in fig. 11;

next, the moving mechanism drives the consumable loading platform 10 to move along the Y-axis direction (which can be automatically moved by using a motor or manually moved by manual transmission), and the moving mechanism is close to the cover opening and closing mechanism 11, so that a small part of the second cornice 9 of the pipe cover plate 8 enters the inlet end of the consumable cover clamping groove 22, meanwhile, the lower edge of the consumable body fixing groove 24 can press the connecting plate 3 of the consumable body 1 and play a role in fixing, and the consumable body 1 is fixed on the consumable loading platform 10, as shown in fig. 12;

next, the consumable loading platform 10 can continue to move along the Y-axis direction, so that the second cornice 9 of the tube cover plate 8 further enters the inlet end of the consumable cover clamping groove 22, and the cover opening protrusion 23a prying cover part slightly pries the consumable cover 2. At this time, the lower edge of the consumable body fixing groove 24 still presses the connection plate 3 of the consumable body 1 for fixing, as shown in fig. 13;

next, the screw motor 15 drives the second operation layer 18 to move upward for a short distance along the Z-axis, so as to increase the force for prying the consumable cover 2 by the cover opening protrusion 23 a. At this time, the four springs between the second operation layer 18 and the third operation layer 19 are still in a compressed state, and the lower edge of the consumable body fixing groove 24 still presses the first cornice 6 of the consumable body 1 to fix the same, as shown in fig. 14;

next, the consumable loading table 10 continues to move along the Y-axis direction until the consumable covers 2 are completely submerged into the consumable cover slots 22, and in this process, the cover-opening protrusions 23a pry all the consumable covers 2. Finally, the consumable lid 2 is fully inserted into the consumable lid slot 22. In addition, in the process, the two fixing bolts 13 respectively arranged at the left side and the right side of the consumable loading platform 10 are respectively clamped into the clamping ports 26 at the two ends of the two fixing strips 25 below the switch cover mechanism 11 to form four-point fixation, as shown in fig. 15;

finally, the screw motor 15 drives the second operation layer 18 to move upwards along the Z-axis, and the consumable cover 2 entering the consumable cover clamping groove 22 at this time is completely pulled up by taking the second cornice 9 as a stress point, as shown in fig. 16. At this time, the four springs between the second working layer 18 and the third working layer 19 are in a compressed state; meanwhile, four-point fixation is formed between the third operation layer 19 and the consumable loading platform 10 through the fixing bolts 13 and the clamping interfaces 26, the lower edge of the consumable body fixing groove 24 is jointly guaranteed to press the first cornice 6 of the consumable body 1, the fixing effect is achieved, and the operation of opening and closing the cover is completed in a matching mode. And simultaneously, the utility model discloses in, because third operation layer 19 and second operation layer 18 piece are provided with elastic component 21, third operation layer 19 can reciprocate, avoids the direct hard contact of third operation layer 19 and consumptive material main part 1, leads to the damage of bumping of on-off mechanism or consumptive material, and the spring has also played the effect of buffering here. After the cover opening operation is completed, the cover body is received in the cover opening protrusion 23a and the connecting block 23b, so that the cover body is prevented from deviating to influence the subsequent cover closing process after the cover opening is completed.

The cover opening bulge 23a comprises a prying cover part and a limiting part; the sledge cover part is positioned at the front end of the uncovering bulge 23a, and the limiting part is positioned at the rear end of the uncovering bulge 23 a. This arch 23a of uncapping can set up to right triangle, and its hypotenuse is the sled lid portion, and the right-angle limit is spacing portion, and the sled lid portion is located the place ahead of spacing portion for insert the gap between consumptive material lid 2 and consumptive material main part 1, then pry consumptive material lid 2, after consumptive material lid 2 is whole to be nested in consumptive material lid draw-in groove 22, spacing portion can restrict consumptive material lid 2 landing. Connecting block 23b is provided with the circular arc chamfer, and this circular arc chamfer plays the guide effect, when avoiding instrument operation precision to deviate, and consumptive material lid bumps with the strip of uncapping firmly and draws the contact. Certainly can alternate in the above-mentioned step of uncapping and set up time interval and elevator motor's along Z axle elevating movement to guarantee that the process of uncapping can more reliably and move high-efficiently, here no longer give unnecessary detail.

The utility model discloses a close and cover the method as follows:

after the experiment is finished, all the nucleic acid reaction consumables are in an open-cover state, and the consumable body 1 is positioned in the consumable clamping groove 12b of the consumable loading platform 10; the consumable lid 2 is nested in the consumable lid slot 22 of the second shelf.

Firstly, the consumable loading table 10 moves to a position corresponding to the inlet end of the opening and closing cover mechanism 11 along the Y-axis direction; next, the screw motor 15 pushes the second operation layer 18 and the third operation layer 19 to move downwards along the Z axis, so that the third operation layer 19 reaches a lower limit position, and at this time, the lower edge of the consumable body fixing groove 24 on the third operation layer 19 corresponds to the connecting plate 3 of the consumable body 1, that is, the lower edge of the consumable body fixing groove 24 is flush with the upper surface of the connecting plate 3; the consumable cover 2 is nested in the second operation layer consumable cover clamping groove 22, and the opening 7 of the tube cover is higher than the height of the tube opening 5 of the consumable main body 1 of the loading platform, as shown in fig. 17;

next, the consumable loading table 10 continues to move in the Y-axis direction until all of the consumable body 1 is submerged into the consumable body fixing groove 24, as shown in fig. 18;

next, the screw motor 15 pushes the second operation layer 18 to move downwards along the Z-axis, so that the consumable cover 2 nested in the consumable cover clamping groove 22 is covered on the consumable body 1; the screw motor 15 continues to push the second operation layer 18 to move downwards along the Z-axis until the pipe cover 7 covers the pipe body 4, and the cover closing operation is completed, as shown in fig. 19;

next, the screw motor 15 drives the second operation layer 18 to move upwards for a short distance along the Z-axis, so that the highest point of the uncovering protrusion is slightly lower than the pipe cover plate 8 of the consumable cover 2, and the consumable loading table 10 exits the cover opening and closing mechanism 11 along the Y-axis direction, as shown in 20;

finally, the consumable loading table 10 exits the cover opening and closing mechanism 11 along the Y-axis direction, and the cover opening and closing mechanism 11 moves upwards along the Z-axis direction to reset.

Therefore, the lifting motor can be used for pushing the second operation layer 18 to move downwards by using different cover opening and closing mechanisms 11 in the cover opening process, the third operation layer 19 also moves downwards, when the third operation layer 19 reaches the limit position, the second operation layer 18 continues to move downwards, the elastic assembly 21 is compressed to apply a downward thrust to the third operation layer 19, the third operation layer 19 directly presses the flying edge 6 of the consumable main body in the cover opening process, the elastic assembly mainly plays a role in playing a role in simultaneously driving the second operation layer 18 and the third operation layer 19 to move upwards by simply rotating the motor reversely, if the spring is lacked, the downward pressing force of the compression spring on the third operation layer 19 is lost, in this case, the separation of the cover body and the consumable body cannot be realized (when the spring is reversely existed, the compression on the consumable body can be realized by using the state of the spring of the motor which reversely rotates at this time, even if the compression state of the spring of the motor which reversely rotates at this time can be continuously maintained, so that the flying force of the consumable body which continuously presses downwards along the flying edge 6 is generated in the cover opening process. More optimally, the lifting motor 15 of the present solution directly or indirectly connects N synchronous operation units directly or indirectly connected to the second operation layer 18 to operate (where N is an integer not less than 2), so that the N operation units apply driving force for driving the second operation layer to move up and down along the vertical direction at multiple points, and thus, the cover opening and closing process can be accurately performed without deflection, and the effect of opening and closing the cover with high precision is achieved.

The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and its core ideas of the present invention. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall into the scope of the claims of the present invention.

It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrases "comprising one of 8230; \8230;" 8230; "does not exclude the presence of additional like elements in a process, method, article, or apparatus that comprises the element.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.

Claims (10)

1. A connecting pipe type nucleic acid reaction consumable material cover opening and closing mechanism is characterized by comprising a first operation layer, a second operation layer and a third operation layer which are sequentially arranged from top to bottom; and a power mechanism arranged therebetween; the power mechanism can drive the second operation layer to move up and down along the vertical direction; a plurality of elastic elements are arranged between the second operation layer and the third operation layer; the switch cover mechanism also comprises a limiting part for limiting the limit position of the third operation layer; when the power mechanism drives the second operation layer to move downwards, the third operation layer also moves downwards, when the third operation layer reaches the limit position, the second operation layer continues to move downwards to enable the elastic element to be compressed, and the elastic element in the compressed state is in close contact with the third operation layer.

2. The consumable opening and closing mechanism for a connected tube type nucleic acid reaction according to claim 1, wherein the actuating mechanism is capable of driving N actuating units, which are directly or indirectly operated on the second operation layer, to move synchronously, wherein N is an integer not less than 2, and the N actuating units simultaneously apply driving forces to the second operation layer to move up and down in a vertical direction at multiple points.

3. The consumable opening and closing mechanism for a tube-connected nucleic acid reaction according to claim 2, wherein the operation unit is a screw unit that can be driven by the power mechanism through a pulley and/or a gear train.

4. The cap opening and closing mechanism for consumable parts for nucleic acid reaction of connected tube type according to claim 3, wherein the screw unit is disposed between the first working layer and the second working layer.

5. The consumable opening/closing mechanism for a connected tube type nucleic acid reaction kit according to claim 1, wherein a plurality of first guide rods are disposed between the first operation layer and the third operation layer, and the third operation layer can move relative to the first operation layer along the length direction of the first guide rods along with the second operation layer under the driving of the power mechanism.

6. The consumable opening/closing cap mechanism for connected tube type nucleic acid reaction according to claim 5, wherein a limiting portion of a limiting position of the third operation layer is provided at an upper end of the first guide rod; the limiting part is positioned above the first operation layer.

7. The consumable opening/closing mechanism for a connected tube type nucleic acid reaction product according to claim 6, wherein the power mechanism is directly or indirectly connected to the second working layer, and the power mechanism body moves up and down along with the second working layer while driving the second working layer to move up and down in the vertical direction.

8. The consumable opening/closing cap mechanism for nucleic acid reaction of connected tube type according to claim 5, wherein the position-limiting portion is provided at a lower end position of the first guide rod; the limiting part is positioned below the third operation layer.

9. The consumable opening/closing mechanism for a connected tube type nucleic acid reaction product according to claim 8, wherein the power mechanism is directly or indirectly connected to the first operation layer, so that the body of the power mechanism is fixed during the process that the power mechanism drives the second operation layer to move up and down in the vertical direction.

10. The lid opening and closing mechanism for the tube-connecting type nucleic acid reaction consumable of claim 5, wherein a plurality of second guide rods are vertically arranged between the second operation layer and the third operation layer; the upper end of the second guide rod penetrates through the second operation layer, and the lower end of the second guide rod is connected with the third operation layer; the elastic element is sleeved on the second guide rod.

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