CN214439178U - High flux liquid-transfering device - Google Patents
High flux liquid-transfering device Download PDFInfo
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- CN214439178U CN214439178U CN202023329620.XU CN202023329620U CN214439178U CN 214439178 U CN214439178 U CN 214439178U CN 202023329620 U CN202023329620 U CN 202023329620U CN 214439178 U CN214439178 U CN 214439178U
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Abstract
The utility model discloses a high-flux liquid-transfering device, which comprises a base, a Z-axis driving mechanism, a liquid-transfering component, a Y-axis driving mechanism and a row of liquid-transfering devices, wherein the Z-axis driving mechanism comprises a stand column and a sliding block which moves up and down, and the liquid-transfering component is connected with the sliding block through a connecting mechanism; the Y-axis driving mechanism comprises a first linear module, the first linear module is arranged on the base along the Y-axis direction, and the bottom of the upright post is fixed on a sliding block of the first linear module; the liquid transfer instrument comprises a liquid transfer suction head placing box, a liquid sample box and a test tube placing box, wherein the liquid transfer suction head placing box comprises a plurality of liquid transfer suction heads placed according to a matrix, and the test tube placing box comprises a plurality of test tubes placed according to the matrix; every row of liquid-transfering apparatus includes three station along the Y axle direction at the base, and the box is placed to liquid-transfering suction head in first station, and the liquid sample box is placed to the second station, and the box is placed to the test tube in third station. The utility model discloses there is three station along Y axle direction, does not need the manual box that trades when moving the liquid, moves liquid work efficiency height.
Description
[ technical field ]
The utility model relates to a move the liquid equipment, especially relate to a high flux moves liquid device.
[ background art ]
Pipetting is an important operation content in the fields of biology, medicine and the like and is one of basic operations of various research experiments. Most of the work related to pipetting titration uses a pipetting gun, most of the processes of taking and discharging liquid of the instruments need manual operation, and the problems of liquid leakage, forgetting to rinse and the like easily occur.
The automatic liquid-transfering equipment is an automatic liquid-processing instrument, can be substituted for traditional liquid-transfering tool, can automatically implement high-accuracy liquid-processing tasks of gradient dilution, liquid-transfering and liquid-merging, and can be combined with detection instrument so as to implement high-effective and accurate detection of target object. The full-automatic operation process of the multi-station high-throughput automatic pipetting equipment can enable experimenters to get rid of complex experimental operation, effectively reduce human errors, improve the repeatability of experiments, reduce the cost, can also carry out process control and tracing, and can be widely applied to bioengineering, DNA plasmid purification, drug screening, ELISA reaction, PCR pretreatment, DNA sequencing treatment, clinical test sample treatment and high-throughput sample analysis of a blood station system.
According to the common operation flow of the medical industry, the automatic pipetting device has a complete pipetting cycle, which mainly comprises the following steps: assembling the liquid-transferring suction head and the liquid-transferring sleeve handle, setting the capacity, pre-washing the liquid-transferring suction head, sucking liquid, discharging liquid (or other complex liquid-transferring operations), and removing the liquid-transferring suction head.
The utility model with the patent number of CN201921356372.2 relates to a high-throughput and high-precision pipetting device, which comprises a mounting rack, a pipetting assembly and a lifting assembly for driving the mounting rack to lift, wherein the pipetting assembly comprises a piston rod base arranged on the mounting rack, a piston top plate positioned on the upper side of the piston rod base and a gun withdrawing head plate positioned on the lower side of the piston rod base; the mounting frame is provided with a driving mechanism for driving the piston top plate to move longitudinally, and the piston rod base is provided with a plurality of gun head rods which penetrate through the gun head withdrawing plate and are used for clamping gun heads, a piston rod matched with the gun head rods, a gun head pressing shaft for driving the gun head withdrawing plate to descend and a resetting piece for resetting the gun head withdrawing plate after descending; the piston rod and the gun pressing head shaft are driven by the piston top plate to move longitudinally; the gun head is installed through the lifting assembly, manual operation is not needed, the automation degree is high, and poor sealing performance between the gun head rod and the gun head caused by manual operation is avoided; the driving mechanism can drive the piston top plate to suck and spray liquid, so that high-flux and high-precision liquid transfer is realized.
However, the utility model discloses a carry flitch only two stations, the during operation needs the manual box that trades, is semi-manual work, and work efficiency is lower.
[ summary of the invention ]
The to-be-solved technical problem of the utility model is to provide a higher high flux of work efficiency moves liquid device.
In order to solve the technical problem, the utility model adopts the technical scheme that the high-throughput pipetting device comprises a base, a Z-axis driving mechanism, a pipetting assembly, a Y-axis driving mechanism and at least one row of pipetting devices, wherein the Z-axis driving mechanism comprises a stand column and a sliding block which moves up and down, and the pipetting assembly is connected with the sliding block through a connecting mechanism; the Y-axis driving mechanism comprises a first linear module, the first linear module is arranged on the base along the Y-axis direction, and the bottom of the upright post is fixed on a sliding block of the first linear module; the liquid transfer instrument comprises a liquid transfer suction head placing box, a liquid sample box and a test tube placing box, wherein the liquid transfer suction head placing box comprises a plurality of liquid transfer suction heads placed according to a matrix, and the test tube placing box comprises a plurality of test tubes placed according to the matrix; every row of liquid-transfering apparatus includes three station along the Y axle direction at the base, and the box is placed to liquid-transfering suction head in first station, and the liquid sample box is placed to the second station, and the box is placed to the test tube in third station.
The high-flux liquid-transfering device comprises two rows of liquid-transfering appliances, the connecting mechanism comprises an X-axis driving mechanism and a mounting plate, the X-axis driving mechanism comprises a second linear module, the second linear module is arranged along the X-axis direction, and one end of the second linear module is mounted on the sliding block; the liquid transfer assembly is arranged on the sliding block of the second linear module through the mounting plate; two rows of pipetting devices are arranged in parallel on the base, separated in the direction of the X axis.
In the high-throughput liquid-transfering device, the first linear module comprises a shell, a Y-axis motor, a synchronous belt transmission device, a driving block and two groups of first linear guide rail pairs, the driving block is arranged above the strip-shaped shell and is of a hollow structure and comprises two side plates, the Y-axis motor is fixed on the side plates of the driving block, and a main shaft of the Y-axis motor extends into an inner cavity of the driving block; the synchronous belt transmission device comprises a synchronous belt, a driving wheel and two tension wheels, the synchronous belt is arranged along the Y-axis direction, and two ends of the synchronous belt are fixed on the shell of the first linear module; the driving wheel and the two tension wheels are arranged in the inner cavity of the driving block, the driving wheel is arranged between the two tension wheels, the bottoms of the two tension wheels are pressed on the top surface of the synchronous belt, the synchronous belt bypasses the top of the driving wheel, and the upper half cycle of the driving wheel is meshed with the inner periphery of the synchronous belt; the guide rails of the two groups of first linear guide rail pairs are horizontally fixed above the shell, and the stand columns of the Z-axis driving mechanism are fixed on the slide blocks of the two groups of first linear guide rail pairs and are connected with the driving block.
In the high-throughput pipetting device, the second linear module comprises a synchronous belt linear sliding table driven by a motor and two groups of second linear guide rail pairs, the synchronous belt linear sliding table is arranged along the X-axis direction, and one end of the synchronous belt linear sliding table is fixed on a sliding block of the Z-axis driving mechanism; two groups of second linear guide rail pairs are arranged above and below one another and are fixed on the synchronous belt linear sliding table along the X-axis direction; the mounting plate is fixed on the sliding blocks of the two groups of second linear guide rail pairs and connected with the synchronous belt of the synchronous belt linear sliding table.
The high-throughput pipetting device comprises a pipetting assembly, a plurality of pipetting modules and a plurality of pipetting sleeve handles, wherein the pipetting assembly comprises a pipetting driving part, a plunger top plate, a plurality of plungers, a plunger cavity, a pipetting head withdrawing mechanism and a plurality of pipetting sleeve handles; the liquid-transferring driving part comprises a liquid-transferring motor and a screw nut component; the mounting frame is in a door shape and comprises a top plate and two side plates; the liquid-transferring motor is arranged on the top plate of the mounting frame; the plunger cavity is vertically fixed at the lower part of the mounting rack and between the two side plates of the mounting rack; the tops of the plungers are connected with a plunger top plate, and the lower parts of the plungers are inserted into plunger cavities corresponding to the plunger cavities; the upper ends of the pipetting sleeve handles are arranged at the bottom of the plug cavity, and the inner holes of the pipetting sleeve handles are communicated with the plunger cavity corresponding to the plunger cavity; the liquid-transfering motor is fixed on the top plate of the mounting frame, a lead screw of the lead screw nut component is connected with the lower end of the liquid-transfering motor, and a nut of the lead screw nut component is connected with the plunger top plate.
In the high-throughput pipetting device, the plunger cavity is connected with the inner sides of the side plates of the mounting rack, the pipetting driving part comprises the motor plate, two groups of third linear guide rail pairs and the driving rack, the driving rack comprises the top plate and two side plates, the motor plate is arranged at the front part of the top plate of the mounting rack, and the pipetting motor is fixed on the motor plate; the nut of the screw-nut component is arranged on the top plate of the driving frame, the guide rail of the third linear guide rail pair is vertically fixed on the inner side of the side plate of the mounting frame, and the slide block of the third linear guide rail pair is fixed on the outer side of the side plate of the driving frame; two ends of the plunger piston top plate are respectively connected with the lower ends of the two side plates of the driving frame.
In the high-throughput pipetting device, the pipette tip withdrawing mechanism comprises the pipette tip withdrawing plate, 4 pressure springs, 4 push rods and a pipette tip withdrawing plate which is a pore plate and comprises pipette sleeve handle holes arranged in a matrix, the pipette tip withdrawing plate is arranged below the cavity of the plunger, and the pipette sleeve handle penetrates through the pipette sleeve handle holes of the pipette tip withdrawing plate; the two sides of the lower part of the plunger cavity respectively comprise a flange, the flange at the lower part of the plunger cavity comprises two push rod holes, and the lower end of the push rod penetrates through the push rod holes of the flange to be connected with the sucker withdrawing plate; the compression spring is sleeved on the push rod, the lower end of the compression spring abuts against the flange at the lower part of the plunger cavity, and the upper end of the compression spring abuts against the head of the push rod; before the driving frame descends to the stroke end point, the lower end of the side plate of the driving frame touches the push rod to push the pipette tip withdrawing plate to descend, and the pipette tip arranged on the pipette sheath handle is pushed downwards by the pipette tip withdrawing plate.
The utility model discloses a high flux moves liquid device has three station along Y axle direction, does not need the manual box that trades during moving the liquid, moves liquid work efficiency height.
[ description of the drawings ]
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Fig. 1 is the front view of the multi-station high-throughput pipetting device of the embodiment of the present invention.
Fig. 2 is the top view of the multistation high throughput liquid-transfering device of the embodiment of the utility model.
Fig. 3 is a left side view of the multi-station high-throughput pipetting device of the embodiment of the present invention.
Fig. 4 is a perspective view of the multi-station high-throughput pipetting device of the embodiment of the present invention.
Fig. 5 is a perspective view of the Y-axis driving mechanism according to the embodiment of the present invention.
Fig. 6 is a cross-sectional view of the Y-axis drive mechanism according to the embodiment of the present invention.
Fig. 7 is a perspective view of the X-axis driving mechanism according to the embodiment of the present invention.
Fig. 8 is a sectional view of a Z-axis drive mechanism according to an embodiment of the present invention.
Fig. 9 is a perspective view of the pipette head and the pipette tip placement box according to the embodiment of the present invention.
Fig. 10 is a front view of the pipette head and the pipette tip placement box according to the embodiment of the present invention.
Fig. 11 is a left side view of the pipette head and pipette tip placement box of the embodiment of the present invention.
Fig. 12 is a top view of a pipetting head according to an embodiment of the invention.
Fig. 13 is a perspective view of the elastic pressing device according to the embodiment of the present invention.
Fig. 14 is a sectional view a-a in fig. 11.
Fig. 15 is a sectional view B-B in fig. 10.
Fig. 16 is a cross-sectional view of a pipetting mechanism according to an embodiment of the present invention.
Fig. 17 is a cross-sectional view of a pipetting sleeve handle according to an embodiment of the invention.
Fig. 18 is a cross-sectional view of a pipetting tip according to an embodiment of the invention.
FIG. 19 is a perspective view of the pipette tip placement box according to the embodiment of the present invention
Fig. 20 is a schematic view of the pipette head according to the embodiment of the present invention.
[ detailed description of the invention ]
The embodiment of the utility model provides a multistation high flux liquid-transfering device's structure is shown in fig. 1 to fig. 20, including base 02, X axle actuating mechanism 10, Y axle actuating mechanism 20, Z axle actuating mechanism 30, move liquid aircraft nose 100 and two and move liquid utensil 200. Pipetting head 100 includes a pipetting mechanism and an insertion mechanism. Each column of pipetting instruments 200 comprises one pipetting tip placement box 90, one liquid sample box 95 and one test tube placement box 96.
The Y-axis driving mechanism 20 is a linear module, and the linear module of the Y-axis driving mechanism 20 is installed on the base 02 along the Y-axis direction. Including casing 21, Y axle motor 22, synchronous belt drive, drive block 23 and two sets of linear guide pair 24, drive block 23 arranges the top at bar casing 21, and for hollow structure, including two curb plates 231, Y axle motor 22 is fixed on the curb plate 231 of drive block 23, and the main shaft of Y axle motor 22 stretches into in the inner chamber of drive block 23. The synchronous belt transmission device comprises a synchronous belt 25, a driving wheel 26 and two tension wheels 27, wherein the synchronous belt 25 is arranged along the Y-axis direction, and two ends of the synchronous belt 25 are fixed on the shell 21 of the linear module; a driving pulley 26 and two tension pulleys 27 are installed in the inner cavity of the driving block 23, the driving pulley 26 is disposed between the two tension pulleys 27, the bottoms of the two tension pulleys 27 press on the top surface of the timing belt 25, the timing belt 25 passes around the top of the driving pulley 26, and the upper half circumference of the driving pulley 26 is engaged with the inner circumference of the timing belt 25. The guide rails 241 of the two linear guide rail pairs 24 are horizontally fixed above the housing 21
The Z-axis driving mechanism 30 includes a column 31, a Z-axis motor 32, a sliding block 33, a sliding block driving device and a counterweight 35, and the sliding block driving device includes a synchronous belt device 36, two linear guide rail pairs 37 and two linear guide rail pairs 38. The guide rails 371 of the two linear guide pairs 37 are vertically installed in front of the vertical column 31, and the sliding blocks 33 are fixed on the sliding blocks 372 of the two linear guide pairs 37. The guide rails 381 of the two linear guide rail pairs 38 are vertically installed at the back of the upright column 31, and the balancing weight 35 is fixed on the sliding blocks 382 of the two linear guide rail pairs 38. The column 31 is a housing 21 structure, and the timing belt device 36 is vertically arranged in a cavity of the housing 21 of the column 31. The Z-axis motor 32 is fixed on the top of the upright column 31, and the driving wheel 361 of the synchronous belt device 36 is arranged on the main shaft of the Z-axis motor 32. The synchronous belt device 36 comprises a connecting block 362 and a connecting block 363, the front wall of the housing of the upright 31 is provided with a vertical through groove 311, the rear wall is provided with a vertical through groove 312, and the connecting block 361 is arranged in the through groove 311 of the front wall and connects the front chord 364 and the sliding block 33 of the synchronous belt. A connecting block 362 is disposed in the through slot 312 of the rear wall, connecting the rear chord 365 of the timing belt and the counterweight 35.
The upright column 31 of the Z-axis driving mechanism 30 is fixed on the slide blocks 242 of the two groups of linear guide rail pairs 24 and is connected with the driving block 23 of the linear module of the Y-axis driving mechanism 20.
The X-axis driving mechanism 10 comprises a linear module, the linear module of the X-axis driving mechanism 10 comprises a synchronous belt linear sliding table 11 and two groups of linear guide rail pairs 12, the synchronous belt linear sliding table 11 is driven by an X-axis motor 13 and is arranged along the X-axis direction, and one end of the synchronous belt linear sliding table is fixed on a sliding block 33 of the Z-axis driving mechanism 30. The guide rails 121 of the two linear guide rail pairs 12 are fixed on the synchronous belt linear sliding table 11 along the X-axis direction.
The insertion mechanism of the pipetting head 100 comprises a mounting plate 01, an elastic pressing device 40, an insertion driving device 50 and a press-fitting force application device 60. The mounting plate 01 of the plug-in mechanism is fixed on the sliding blocks 122 of the two linear guide rail pairs 12 and is connected with the synchronous belt of the synchronous belt linear sliding table 11.
The elastic pressing device 40 comprises two groups of linear guide rail pairs 41, three compression springs 42, three compression spring guide columns 43, an upper top plate 44, a lower top plate 45, an L-shaped connecting plate 46 and a limiting block 47. The upper top plate 44 and the lower top plate 45 are transversely arranged, the lower ends of the pressure spring guide posts 43 are fixed on the lower top plate 45, and the three pressure spring guide posts 43 are transversely and separately arranged along the lower top plate 45. The upper top plate 44 includes three guide post holes 441, and the guide post holes 441 are arranged separately in the lateral direction of the upper top plate 44. The upper top plate 44 is fixed in front of the mounting plate 01, and the upper end of the pressure spring guide post 43 passes through the guide post hole 441 of the upper top plate 44 and is in sliding fit with the guide post hole 441. The pre-tightening compression spring 42 is sleeved outside the compression spring guide post 43 and is positioned between the upper top plate 44 and the lower top plate 45. The guide rails 411 of the two linear guide rail pairs 41 are vertically fixed in front of the mounting plate 01 and are separately arranged on both sides of the upper top plate 44 and the lower top plate 45. The L-shaped connecting plate 46 is disposed below the lower top plate 45, the lower top plate 45 is fixed on a vertical plate of the L-shaped connecting plate 46, and two ends of the L-shaped connecting plate 46 are respectively fixed on the sliding blocks 412 of the two sets of linear guide rail pairs 41. The stop block 47 is fixed to the front of the mounting plate 01 below the L-shaped connecting plate 46.
The insertion driving device 50 comprises an insertion motor 51, a door-shaped motor base 52, a screw nut assembly 53 and two groups of linear guide rail pairs 54. The door-shaped motor base 52 comprises a top plate 521 and two side plates 522, wherein the top plate 521 of the motor base 52 is fixed on the transverse plate of the L-shaped connecting plate 46 of the elastic pressing device 40. The cartridge motor 51 is vertically fixed on the top surface of the top plate 521 of the motor base 52, and the lead screw 531 of the lead screw nut assembly 53 is connected with the lower end of the cartridge motor 51.
The press-fitting force application device 60 comprises two groups of hooks 60A and hook closing mechanisms 60B for hooking the bearing flanges of the box body 91 of the pipette tip placement box 90.
Each set of hooks 60A comprises a connecting rod 61, a slider 62 and two hook rods 63, the lower ends of the hook rods 63 are provided with a hook head 631, and the hook heads 631 face inwards. The upper ends of the two hook rods 63 are fixed to the front and rear ends of the connecting rod 61, respectively, and the slider 62 is fixed to the bottom surface of the middle portion of the connecting rod 61. The two sides of the top surface of the top plate 521 of the motor seat 52 are respectively provided with a sliding groove 523 which is transversely arranged, the connecting rod 61 is arranged above the top plate of the motor seat 52 and is fixed on the sliding block 62 at the bottom surface of the connecting rod 61 to be in sliding fit with the sliding groove 523, and the hook 60A can transversely move along the sliding groove 523.
The hook folding mechanism 60B includes two sets of hook folding mechanisms symmetrically arranged left and right, and each set of hook folding mechanism includes a guide rod 64, a guide sleeve seat 65, a pressure spring 66, a guide plate 67 and a feeler lever 68. The guide rod 64 is transversely arranged above the top plate 521 of the motor seat 52, the inner side of the guide rod is in sliding fit with a guide sleeve in a guide sleeve seat 65, and the guide sleeve seat 65 is fixed above the top plate 521 of the motor seat 52. The outer end of the guide rod 64 is fixed to the connecting rod 61 with a fixing seat 69. The middle part of the guide rod 64 is provided with a flange 641, and the pressure spring 66 is sleeved on the guide rod 64 and is positioned between the guide sleeve seat 65 and the flange 641. The front end of the feeler lever 68 is fixed to the flange 641 of the guide 64. The guide plate 67 is disposed diagonally in front of the mounting plate 01, above and outside the rear end of the trolley 68. The inner side of the guide plate 67 is a guide surface, and the rear end of the feeler lever 68 is kept in contact with the guide surface of the guide plate 67 by the elastic force of the compression spring 66.
The pipetting mechanism of pipetting head 100 includes vertically arranged pipetting assembly 70, pipetting drive member 80, mounting bracket 88 and pipetting head mechanism 89.
The pipetting driving part 80 comprises a pipetting motor 81, a screw nut component 82, a motor plate 83, two groups of linear guide rail pairs 84 and a driving frame 85.
The mounting bracket 88 is door-shaped and includes a top plate 881 and two side plates 882. The guide 541 of the linear guide pair 54 is vertically fixed on the inner side of the side plate 522 of the motor base 52, and the slider 542 of the linear guide pair 54 is fixed on the outer side of the side plate 882 of the mounting frame 88. The nut 532 of the lead screw nut assembly 53 is mounted on the top plate 881 of the mounting bracket 88.
The motor plate 83 is fixed to the front portion of the top plate 881 of the mounting rack 88, and the liquid-transferring motor 81 is fixed to the motor plate 83. The lead screw 821 of the lead screw nut assembly 82 is connected with the lower end of the liquid-transferring motor 81.
The driving frame 85 comprises a top plate 851 and two side plates 852, the nut 822 of the screw nut assembly 82 is installed on the top plate 851 of the driving frame 85, the guide rail 841 of the linear guide rail pair 84 is vertically fixed on the inner side of the side plate 882 of the mounting frame 88, and the slide block of the linear guide rail pair 84 is fixed on the outer side of the side plate 852 of the driving frame 85. The two ends of the plunger top plate 71 are respectively connected with the lower ends of the two side plates of the driving frame 85.
As shown in fig. 10, the pipette tip ejecting mechanism 89 includes a pipette tip ejecting plate 891, 4 compression springs 892, 4 push rods 893, and a pipette tip ejecting plate 891 which is a perforated plate including handle holes arranged in an 8 × 12 matrix, the pipette tip ejecting plate 891 is arranged below the plunger cavity 73, and the pipette handle 74 passes through the handle holes of the pipette tip ejecting plate 891. The plunger cavity 73 includes a flange 732 on each side of the lower portion thereof, the flange 732 includes two push rod holes, and the lower end of the push rod 893 passes through the push rod holes of the flange 732 to be connected to the ejector plate 891. A compression spring 892 is fitted over the pushrod 893, with a lower end against the flange 732 and an upper end against the head of the pushrod 893. Before the two side plates 852 of the driving rack 85 descend to the stroke end, the lower ends of the side plates 852 touch the push rods 893 to push the pipette tip withdrawing plate 891 to descend, and the pipette tip withdrawing plate 891 pushes all the used pipette tips 93 downwards to separate from the pipette sheath handle 74.
As shown in fig. 17, the lower portion of the pipetting sleeve 74 is conical with a taper angle of 10 °. As shown in fig. 18, the upper portion of the pipetting tip 93 is formed in an inverted cone shape, the upper portion of the tip inner hole 931 is formed in an inverted cone shape, and the cone angle of the inverted cone shape is matched with the cone angle of the lower portion of the pipetting sleeve holder 74. A circle of inward-protruding interference fit annular belt 932 is arranged in the middle of the inverted cone hole, and the inward protruding height of the interference fit annular belt 932 is 0.1 mm.
As shown in fig. 19, the pipette tip placement cassette 90 includes a cassette body 91, a well plate 92, and 96 pipette tips 93 arranged in an 8 × 12 matrix on the well plate 92. The upper edges of two opposite side plates of the box body 91 of the pipetting tip placing box 90 are respectively provided with a bearing flange 911 which protrudes outwards, and the hook heads 631 of the two groups of hooks 60A are positioned below the outer side of the bearing flange 911. The bottom surface of the box body 91 is provided with a plurality of threaded holes, and the box body 91 can be fixed on the base 02 by the threaded holes on the bottom surface of the box body 91.
Two rows of liquid-transfering instruments 200 are separately arranged along the X-axis direction, each row of liquid-transfering instruments 200 comprises three stations along the Y-axis direction on a base 02, a liquid-transfering tip placing box 90 is placed at the first station, a liquid sample box 95 is placed at the second station, a test tube placing box 96 is placed at the third station, and the test tube placing box 96 is provided with test tubes 961 (or deep hole plates) which are arranged in an 8X 12 matrix according to the moment.
The embodiment of the utility model provides a move liquid aircraft nose 100 and implement the working process of the cartridge of cover handle and suction head as follows:
1) the X-axis driving mechanism and the Y-axis driving mechanism move the liquid-transfering machine head 100 to the upper part of a liquid-transfering sucker placing box 90, and the Z-axis driving mechanism drives the liquid-transfering machine head 100 to descend until the lower end of the liquid-transfering sleeve handle 74 reaches the position of about 2mm of the upper end surface of the sucker; the insertion motor 51 rotates, the feed screw nut component 53 drives the liquid-transferring mechanism to move downwards, the liquid-transferring sleeve handle 74 is pre-inserted into an inner hole of the liquid-transferring suction head 93, and the pressure of the liquid-transferring sleeve handle 74 pre-inserted into the liquid-transferring suction head 93 is the pre-tightening pressure of the elastic pressing device 40;
2) after the liquid-transferring sleeve handle 74 is inserted into the liquid-transferring sucker 93, the insertion motor 51 continues to rotate, the downward movement of the liquid-transferring sleeve handle 74 is blocked, the motor base 52 moves upwards by taking the nut of the screw-nut component 53 as a fulcrum, the three pressure springs 42 of the elastic pressing device 40 are compressed, the motor base 52 drives the two groups of hooks to move upwards, and the hook folding mechanism 60B is triggered at the same time; the feeler lever 68 of the hook closing mechanism 60B moving upwards along the motor base 52 moves inwards along the guide surface of the guide plate 67, the guide rod 64 drives the two groups of hooks to close, and the hook heads 631 of the two groups of hooks hook the two bearing flanges 911 of the box body 91 of the liquid-removing suction head placing box 90;
3) the plugging motor 51 further rotates, because the hook heads 631 of the two groups of hooks already hook the box body 91 of the liquid-transferring suction head placing box 90, the motor base 52 cannot move upwards relative to the liquid-transferring suction head placing box 90, the plugging motor 51 can only drive the liquid-transferring mechanism to move downwards, the interference resistance between the liquid-transferring suction head 93 and the liquid-transferring sleeve handle 74 is overcome, the liquid-transferring sleeve handle 74 is pressed into the inner hole of the liquid-transferring suction head 93, and the press-fitting of the liquid-transferring suction head 93 and the liquid-transferring sleeve handle 74 is completed;
4) the insertion motor 51 rotates reversely, and the hook folding mechanism 60B firstly releases the hook pipette tip 93 to put two groups of hooks: the elastic pressing device 40 pushes the motor base 52 to drive the two groups of hooks to move downwards and trigger the hook folding mechanism 60B at the same time; the feeler lever 68 moving downwards along the motor base 52 moves outwards along the guide surface of the guide plate 67 under the action of the second spring, the guide rod 64 drives the two groups of hooks to open, and the hook heads 631 of the two groups of hooks release the two bearing flanges 911 of the box body 91 of the liquid-removing suction head placing box 90;
5) in the process of releasing the box body 91 of the pipetting tip placing box 90 by the hook heads 631 of the two groups of hooks, the inserting motor 51 drives the pipetting mechanism to move upwards through the screw nut component 53, the inserting mechanism resets, the pipetting sleeve handle 74 of the pipetting component 70 drives the pipetting tip 93 to be drawn out from the box body 91 of the pipetting tip placing box 90, the Z-axis driving mechanism of the pipetting device drives the assembling mechanism to ascend, the tip inserting work is completely finished, and the next liquid extracting work can be carried out.
The utility model discloses in the cartridge working process of the cover handle of above embodiment and suction head, to move the back in the cover handle of liquid subassembly inserts the hole of liquid suction head, the couple of insertion equipment grabs the bearing flange of the box body that liquid suction head placed the box, further exerts pressure to moving the liquid subassembly, will move liquid the liquid handle pressure equipment of liquid subassembly lower part again to move in the hole of liquid suction head. The downward press-fitting force of the liquid-transferring component is balanced by the pulling force applied to the bearing flange by the hook, and cannot be transmitted to an X-axis driving mechanism and a Z-axis driving mechanism of the liquid-transferring equipment through the mounting plate, so that the X-axis driving mechanism and the Z-axis driving mechanism deform to influence the insertion effect of a suction head and a sleeve handle of the high-throughput liquid-transferring equipment.
The embodiment of the utility model provides a multistation high flux liquid-transfering device moves liquid work process explains with first row liquid-transfering appliance 200A as an example, and liquid-transfering process that second row liquid-transfering appliance 200B is the same, and the description is not repeated:
1) the X-axis driving mechanism and the Y-axis driving mechanism move the liquid-transfering machine head 100 to the position above the liquid-transfering suction head placing box 90A at the first station of the first row of liquid-transfering instruments 200A, and the Z-axis driving mechanism drives the liquid-transfering machine head 100 to descend until the lower end of the liquid-transfering sleeve handle 74 reaches about 2mm of the upper end surface of the suction head;
2) the press-fitting process of the liquid-transferring sleeve handle 74 and the first liquid-transferring suction head 93 is completed by the action of the inserting mechanism of the liquid-transferring machine head 100, the liquid-transferring machine head 100 is driven by the Z-axis driving mechanism to lift, and the lower end of the first liquid-transferring suction head 93 leaves the box body 91 of the liquid-transferring suction head placing box 90A;
3) the Y-axis driving mechanism drives the liquid transfer machine head 100 to move to the position above the liquid sample box 95A at the second station along the Y axis, the Z-axis driving mechanism drives the liquid transfer machine head 100 to descend, the first liquid transfer suction head 93 is inserted into liquid in the liquid sample box 95A, the liquid transfer mechanism of the liquid transfer machine head 100 acts to suck quantitative liquid in the liquid sample box 95A, and the Z-axis driving mechanism drives the liquid transfer machine head 100 to ascend;
4) the Y drive mechanism drives and moves liquid aircraft nose 100 and places the top of box 96A along the Y axle removal to third station test tube, Z axle drive mechanism drives and moves liquid aircraft nose 100 and descends, move liquid suction head 93 and insert the test tube and place in the test tube 961 of box 96A (or the deep hole of deep hole board), move liquid aircraft nose 100 move liquid mechanism action, arrange the test tube and place in the test tube 961 of box 96A (or the deep hole of deep hole board) with having absorbed quantitative liquid, Z axle drive mechanism drives and moves liquid aircraft nose 100 and rise, Y drive mechanism drives and moves liquid aircraft nose 100 and reset.
The utility model discloses the work process that above embodiment multistation high flux pipettor moved the liquid does not need the manual work to change the box, can the automation mechanized operation, and work efficiency is high.
The embodiment of the utility model provides a multistation high flux moves liquid device ration and mixes the working process of liquid still includes following step on the basis of the working process that moves the liquid in front:
5) after the Y-axis driving mechanism drives the liquid-transfering machine head 100 to reset along the Y-axis direction, the liquid-transfering machine head 100 is moved to the upper side of the first row of liquid-transfering instruments 200A and the first station liquid-transfering suction head placing box 90A, the Z-axis driving mechanism drives the liquid-transfering machine head 100 to descend, and the first liquid-transfering suction head 93 is inserted into the pore plate 92 of the first station liquid-transfering suction head placing box 90A;
6) the pipette head withdrawing mechanism 89 of the pipetting mechanism of the pipette head 100 operates, and 96 pipette tips 93 are all unloaded into the orifice plate 92 of the first station pipette tip placing box 90A;
7) the Z-axis driving mechanism drives the liquid-transfering machine head 100 to lift, the X-axis driving mechanism drives the liquid-transfering machine head 100 to move to the second row of liquid-transfering apparatus 200B above the liquid-transfering suction head placing box 90B at the first station,
8) the insertion mechanism of the liquid-transfering head 100 acts to complete the press-fitting process of the liquid-transfering sleeve handle 74 and the second liquid-transfering head 93 in the liquid-transfering head placing box 90B, the Z-axis driving mechanism drives the liquid-transfering head 100 to rise, and the lower end of the second liquid-transfering head 93 leaves the box body 91 of the liquid-transfering head placing box 90B;
9) the Y-axis driving mechanism drives the liquid transfer machine head 100 to move to the position above the liquid sample box 95B of the second row of second stations along the Y axis, the Z-axis driving mechanism drives the liquid transfer machine head 100 to descend, the second liquid transfer suction head 93 is inserted into liquid in the liquid sample box 95B, the liquid transfer mechanism of the liquid transfer machine head 100 acts to suck quantitative liquid in the liquid sample box 95B, and the Z-axis driving mechanism drives the liquid transfer machine head 100 to ascend;
10) the Y-axis driving mechanism drives the liquid-transfering head 100 to move to the upper side of the first row of third station test tube placing box 96A along the Y-axis, the Z-axis driving mechanism drives the liquid-transfering head 100 to descend, the second liquid-transfering suction head 93 is inserted into the test tube 961 (or the deep hole of the deep hole plate) of the test tube placing box 96A, the liquid-transfering mechanism of the liquid-transfering head 100 acts to discharge the quantitative liquid in the sucked liquid sample box 95B into the test tube 961 (or the deep hole of the deep hole plate) of the test tube placing box 96A;
11) the Z-axis driving mechanism drives the pipetting head 100 to lift, and the Y-driving mechanism and the X-driving mechanism drive the pipetting head 100 to reset.
The utility model discloses the ration of two kinds of different liquid of above embodiment multistation high flux liquid-transfering device is got liquid and the hybrid process does not need the manual work to trade the box, can the automation mechanized operation, once only accomplishes, and work efficiency is high.
Claims (7)
1. A high-flux liquid-transfering device comprises a base, a Z-axis driving mechanism and a liquid-transfering component, wherein the Z-axis driving mechanism comprises an upright post and a sliding block which moves up and down, and the liquid-transfering component is connected with the sliding block through a connecting mechanism; the automatic liquid transferring device is characterized by comprising a Y-axis driving mechanism and at least one row of liquid transferring devices, wherein the Y-axis driving mechanism comprises a first linear module, the first linear module is arranged on a base along the Y-axis direction, and the bottom of an upright post is fixed on a sliding block of the first linear module; the liquid transfer instrument comprises a liquid transfer suction head placing box, a liquid sample box and a test tube placing box, wherein the liquid transfer suction head placing box comprises a plurality of liquid transfer suction heads placed according to a matrix, and the test tube placing box comprises a plurality of test tubes placed according to the matrix; every row of liquid-transfering apparatus includes three station along the Y axle direction at the base, and the box is placed to liquid-transfering suction head in first station, and the liquid sample box is placed to the second station, and the box is placed to the test tube in third station.
2. High throughput pipetting device according to claim 1, comprising two rows of said pipetting means, said connection means comprising an X-axis drive mechanism and a mounting plate, the X-axis drive mechanism comprising a second linear module arranged in the X-axis direction with one end mounted on said slide; the liquid transfer assembly is arranged on the sliding block of the second linear module through the mounting plate; two rows of pipetting devices are arranged in parallel on the base, separated in the direction of the X axis.
3. The high throughput pipetting device according to claim 1, wherein the first linear module comprises a housing, a Y-axis motor, a synchronous belt transmission device, a driving block and two sets of first linear guide rail pairs, the driving block is arranged above the strip-shaped housing and is of a hollow structure and comprises two side plates, the Y-axis motor is fixed on the side plates of the driving block, and a main shaft of the Y-axis motor extends into an inner cavity of the driving block; the synchronous belt transmission device comprises a synchronous belt, a driving wheel and two tension wheels, the synchronous belt is arranged along the Y-axis direction, and two ends of the synchronous belt are fixed on the shell of the first linear module; the driving wheel and the two tension wheels are arranged in the inner cavity of the driving block, the driving wheel is arranged between the two tension wheels, the bottoms of the two tension wheels are pressed on the top surface of the synchronous belt, the synchronous belt bypasses the top of the driving wheel, and the upper half cycle of the driving wheel is meshed with the inner periphery of the synchronous belt; the guide rails of the two groups of first linear guide rail pairs are horizontally fixed above the shell, and the stand columns of the Z-axis driving mechanism are fixed on the slide blocks of the two groups of first linear guide rail pairs and are connected with the driving block.
4. The high throughput pipetting device according to claim 2, wherein the second linear module comprises a synchronous belt linear slide table driven by a motor and two sets of second linear guide rail pairs, the synchronous belt linear slide table is arranged along the X-axis direction, and one end of the synchronous belt linear slide table is fixed on a sliding block of the Z-axis driving mechanism; two groups of second linear guide rail pairs are arranged above and below one another and are fixed on the synchronous belt linear sliding table along the X-axis direction; the mounting plate is fixed on the sliding blocks of the two groups of second linear guide rail pairs and connected with the synchronous belt of the synchronous belt linear sliding table.
5. High throughput pipetting device according to claim 4, wherein the pipetting assembly comprises a pipetting drive part, a plunger top plate, a plurality of plungers, a plunger chamber, an aspiration head withdrawal mechanism and a plurality of pipetting sleeves; the liquid-transferring driving part comprises a liquid-transferring motor and a screw nut component; the mounting frame is in a door shape and comprises a top plate and two side plates; the liquid-transferring motor is arranged on the top plate of the mounting frame; the plunger cavity is vertically fixed at the lower part of the mounting rack and between the two side plates of the mounting rack; the tops of the plungers are connected with a plunger top plate, and the lower parts of the plungers are inserted into plunger cavities corresponding to the plunger cavities; the upper ends of the pipetting sleeve handles are arranged at the bottom of the plug cavity, and the inner holes of the pipetting sleeve handles are communicated with the plunger cavity corresponding to the plunger cavity; the liquid-transfering motor is fixed on the top plate of the mounting frame, a lead screw of the lead screw nut component is connected with the lower end of the liquid-transfering motor, and a nut of the lead screw nut component is connected with the plunger top plate.
6. The high throughput pipetting device according to claim 5, wherein the plunger cavity is connected with the inner side of the side plate of the mounting rack, the pipetting driving part comprises a motor plate, two sets of third linear guide rail pairs and a driving rack, the driving rack comprises a top plate and two side plates, the motor plate is arranged in front of the top plate of the mounting rack, and the pipetting motor is fixed on the motor plate; the nut of the screw-nut component is arranged on the top plate of the driving frame, the guide rail of the third linear guide rail pair is vertically fixed on the inner side of the side plate of the mounting frame, and the slide block of the third linear guide rail pair is fixed on the outer side of the side plate of the driving frame; two ends of the plunger piston top plate are respectively connected with the lower ends of the two side plates of the driving frame.
7. High throughput pipetting device according to claim 6, wherein the pipetting head withdrawing mechanism comprises a pipetting head plate, 4 pressure springs, 4 push rods, the pipetting head plate is a hole plate and comprises pipetting sleeve handle holes arranged in a matrix, the pipetting head plate is arranged below the plunger cavity, and the pipetting sleeve handle passes through the pipetting sleeve handle holes of the pipetting head plate; the two sides of the lower part of the plunger cavity respectively comprise a flange, the flange at the lower part of the plunger cavity comprises two push rod holes, and the lower end of the push rod penetrates through the push rod holes of the flange to be connected with the sucker withdrawing plate; the compression spring is sleeved on the push rod, the lower end of the compression spring abuts against the flange at the lower part of the plunger cavity, and the upper end of the compression spring abuts against the head of the push rod; before the driving frame descends to the stroke end point, the lower end of the side plate of the driving frame touches the push rod to push the pipette tip withdrawing plate to descend, and the pipette tip arranged on the pipette sheath handle is pushed downwards by the pipette tip withdrawing plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023329620.XU CN214439178U (en) | 2020-12-31 | 2020-12-31 | High flux liquid-transfering device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023329620.XU CN214439178U (en) | 2020-12-31 | 2020-12-31 | High flux liquid-transfering device |
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| Publication Number | Publication Date |
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| CN214439178U true CN214439178U (en) | 2021-10-22 |
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| CN202023329620.XU Expired - Fee Related CN214439178U (en) | 2020-12-31 | 2020-12-31 | High flux liquid-transfering device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114225989A (en) * | 2021-12-29 | 2022-03-25 | 无锡科智达科技有限公司 | Full-automatic liquid transferring and preparing device and process |
| CN114395473A (en) * | 2021-11-08 | 2022-04-26 | 温州经川科技有限公司 | Move liquid and divide appearance equipment |
-
2020
- 2020-12-31 CN CN202023329620.XU patent/CN214439178U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395473A (en) * | 2021-11-08 | 2022-04-26 | 温州经川科技有限公司 | Move liquid and divide appearance equipment |
| CN114225989A (en) * | 2021-12-29 | 2022-03-25 | 无锡科智达科技有限公司 | Full-automatic liquid transferring and preparing device and process |
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