CN206814737U - A kind of nucleic acid extraction purification devices - Google Patents
A kind of nucleic acid extraction purification devices Download PDFInfo
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- CN206814737U CN206814737U CN201720582714.7U CN201720582714U CN206814737U CN 206814737 U CN206814737 U CN 206814737U CN 201720582714 U CN201720582714 U CN 201720582714U CN 206814737 U CN206814737 U CN 206814737U
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Abstract
The utility model discloses a kind of nucleic acid extraction purification devices, including multiple reaction chamber and foranalysis of nucleic acids units on matrix and matrix, by by reagent necessary to nucleic acid extraction, encapsulate, integrate respectively, being integrated in the system of a closing, under the outer power cooperation that necessary instrument is applied, sequentially realize nucleic acid extraction purifying and follow-up detection and analysis process, simplify nucleic acid extraction flow, accelerate extraction rate, the cross pollution of sample room is avoided simultaneously, and realizes the complex process that nucleic acid extraction is implemented automatically from a step to next step.
Description
Technical field
A kind of nucleic acid extraction purification art is the utility model is related to, is filled more particularly, to one kind based on a dimensional double-sided disc type
The nucleic acid extraction purification devices put and realized under centrifugal force driving.
Background technology
Nucleic acid be by many nucleotide polymerizations into large biological molecule compound, be organism hereditary information carrier,
One of most basic material for life.Therefore the research of nucleotide sequence and structure is either carried out, or to gene function, expression
Explored, also or carry out genetic engineering, protein engineering, be required for that nucleic acid is separated and purified first；Meanwhile nucleic acid
The quality of extraction product will also be directly connected to the success or failure of subsequent experimental.Therefore the extraction and purifying of nucleic acid are molecular biology realities
Test operation most important in technology and most basic.
It is varied to the method for nucleic acid extraction and purifying, it is a variety of to can relate to biology, chemistry, machinery, calorifics or acoustics etc.
Different processing modes.But no matter handled, be substantially all including cell inclusion release, nucleic acid separation using which kind of mode
These key steps are discharged in buffer solution with purifying, the precipitation of nucleic acid or absorption and nucleic acid, and these steps are almost every
Different reactions occurs between plurality of reagents for the individual cooperation for being required for different reagents, sample, so as to realize final point of nucleic acid
From and purifying.These steps are various and complicated, and conventional method is handled by hand in the lab by professional, however,
The efficiency of these manual processing methods is low, it is high to spend and operating error be present.With different automaticity nucleic acid extraction instruments
Continue to bring out, lab assistant can utilize nucleic acid extraction instrument handle multiple different samples simultaneously, but how by core
Reagent necessary to acid extraction, encapsulate, integrate respectively, being integrated in the system of a closing, simplifying nucleic acid extraction flow, accelerating
Extraction rate, while the cross pollution of sample room is avoided, and realize that nucleic acid extraction is automatically real from a step to next step
The complex process apparatus and method applied, are still to be expected by market, and anxious pending exploitation.
Utility model content
The defects of the purpose of this utility model is to overcome prior art, there is provided a kind of nucleic acid extraction purification devices, be based on
One has certain thickness, dimensional double-sided disc type locking device, and under the outer power cooperation that necessary instrument is applied, sequentially realizes
Nucleic acid extraction purifies and follow-up detection and analysis process.
To achieve the above object, the utility model proposes following technical scheme：A kind of nucleic acid extraction purification devices, its feature
It is, it includes：Matrix, and be arranged on matrix multiple reagent chambers, nucleic acid absorption chamber, waste chamber, product chamber
With foranalysis of nucleic acids unit, the reagent chamber, waste chamber and product chamber pass through corresponding runner and nucleic acid absorption chamber phase
Logical, waste chamber and the product chamber is communicated by the air flue that flows back accordingly with each reagent chamber, each chamber, runner and
The air flue that flows back is located at the front of matrix, reverse side or set through matrix, the reagent chamber close to the center of matrix；And institute
State reagent chamber, nucleic acid absorption chamber, waste chamber and foranalysis of nucleic acids unit, or the reagent chamber, nucleic acid absorption chamber,
Product chamber and foranalysis of nucleic acids unit are set gradually from inside to outside in the radial direction of matrix, the foranalysis of nucleic acids unit and product chamber
It is connected；Fluid sample, successively by reagent chamber, flows through nucleic acid absorption chamber under centrifugal action caused by matrix rotation,
And waste chamber and product chamber are flowed to respectively, nucleic acid extraction purge process is completed, after finally being carried out in foranalysis of nucleic acids unit
Continuous detection and analysis.
Preferably, the reagent chamber includes the first reagent chamber, at least one second reagent chamber and the 3rd reagent chamber
Room, the adding mouth for adding liquid sample is provided with first reagent chamber, and pre-packaged has cell pyrolysis liquid and pre-
Leave the space needed for for adding liquid sample；Second reagent chamber is pre-packaged cleaning solution；3rd reagent chamber
Room is pre-packaged the nucleic acid eluents for eluting nucleic acid substances from nucleic acid absorption chamber.
Preferably, in first reagent chamber can the volume of adding liquid sample be 100ul~500ul.
Preferably, the reagent chamber passes through public or be connected with nucleic acid absorption chamber by respective reagent runner
It is logical；The waste chamber is connected by waste liquid runner with nucleic acid absorption chamber；The product chamber passes through product runner and core
Acid absorption chamber is connected；The waste chamber is also connected by the first reagent backflow air flue with reagent chamber；The product
Chamber is also connected by the second reagent backflow air flue with reagent chamber；The foranalysis of nucleic acids unit by analyze backflow air flue with
Product chamber is connected.
Preferably, reagent flow road junction is provided with each reagent chamber and the first reagent corresponding with reagent flow road junction returns
Gas road junction, reagent flow road junction communicate with reagent runner, the first reagent backflow gas port and the first reagent backflow air flue or second
Reagent backflow air flue communicates.
Preferably, the reagent flow road junction in each reagent chamber is close to the lateral wall setting of reagent chamber, and flow back air flue
The madial wall that mouth is close to reagent chamber is set.
Preferably, the reagent flow road junction in each reagent chamber is outwards recessed the semicircle of formation by the lateral wall from chamber
Arcuation side groove and the circular groove composition for the formation that is recessed downwards from chamber bottom surface.Backflow gas port in each reagent chamber is equal
It is the circular groove of formation of being recessed downwards from chamber bottom surface.
Preferably, each reagent chamber is corresponded in the reagent runner and is provided with reagent runner valve, in the product runner
Product runner valve is provided with, is provided with and reagent runner valve phase in the first reagent backflow air flue and the second reagent the backflow air flue
Corresponding backflow airway valve.
Preferably, the reagent runner valve in the reagent runner is opened successively, different reagents is sequentially flowed through nucleic acid successively
Adsorb chamber；Reagent runner valve open while, the first reagent backflow air flue or the second reagent backflow air flue in reagent runner
The corresponding backflow airway valve of valve is opened simultaneously.
Preferably, the reagent chamber, foranalysis of nucleic acids cell distribution are in matrix wherein on end face；The waste liquid chamber
Room, product chamber, it is distributed on the other end of matrix；The nucleic acid absorption chamber runs through matrix tow sides.
Preferably, the runner is located at the one side of matrix, two chambers of the connection in device the same face；Or runner is crossed over
Matrix tow sides, two chambers of the connection in device tow sides.
Preferably, the analysis runner is located at wherein on end face of matrix；The reagent runner is located at the another of matrix
On end face；The product runner, waste liquid runner and backflow air flue run through the tow sides of matrix.
Preferably, described matrix is supporting by being fixed on for the rotary fixing structure of fixing device and driving device rotation
In instrument, necessary instrument is under outside mechanical force, and band kinetoplast is unidirectional or crankmotion.
Preferably, the rotary fixing structure includes at least one rotating shaft neck and/or at least one being arranged on matrix
Individual rotation fixed card slot, the rotating shaft neck are located at the center of matrix and are arranged concentrically with matrix, the rotation fixed card slot
It is arranged on the outside of reagent chamber and is set close to the outer side edges of matrix, the rotation fixed card slot runs through matrix.
Preferably, the runner, valve set in backflow air flue be puncture valve, and the puncture valve in outside, tie by pressing
It will be punctured in the presence of structure, the runner, backflow air flue will be switched on.The outside pressing structure can be with one stream of a secondary control
Road, flow back air flue conducting, the multiple runners of a secondary control that can also combine, backflow air flue while turn on and closure.
Preferably, the runner, valve set in backflow air flue are heat-sealing valve, and the heat-sealing valve is in external heat
It will be opened under effect, heat again, the valve will be again switched off.
Preferably, before using the utility model device, set valve will close in all runners, backflow air flue,
After adding mouth adding liquid sample, adding mouth is sealed by sealing.
Preferably, the rotating speed size of described device rotation is 2000rpm~10000rpm.
Preferably, the material of described matrix is made up of at least one plastics or resin material, the plastics or resin material
Including polyethylene, low density polyethylene (LDPE), polypropylene, polyvinyl chloride.
Preferably, generally with certain thickness, two-sided processing, the three-dimensional tray type structure of described matrix, in part area
Domain is the single layer structure that thickness is less than other regions.
Preferably, the reagent chamber, nucleic acid absorption chamber, waste chamber, product chamber are by least one layer of plastic film
Material plastic packaging is formed in rigid plastics material, or is sealed and formed by the membranaceous plastic material of at least two layers of thin.The shape of each chamber
Shape can be but be not limited to fan-shaped, circular, ellipse, water-drop-shaped or irregular shape.
Preferably, the runner is formed by least one layer of plastic film material plastic packaging in rigid plastics material, or by extremely
Few membranaceous plastic material of two layers of thin seals to be formed.The shape of the runner can be but be not limited to linear, circular arc or irregular
Shape.
Compared with prior art, the beneficial effects of the utility model are：The utility model will try necessary to nucleic acid extraction
Agent, encapsulate, integrate respectively, being integrated in the system of a closing, under the outer power cooperation that necessary instrument is applied, sequentially realizing
Nucleic acid extraction purifies and follow-up detection and analysis process, simplifies nucleic acid extraction flow, accelerates extraction rate, while avoid sample
Between cross pollution, and realize the complex process that nucleic acid extraction is implemented automatically from a step to next step.
Brief description of the drawings
Fig. 1 represents the A facial plane schematic diagrames of device in the utility model embodiment 1；
Fig. 2 represents the A faces schematic perspective view of device in the utility model embodiment 1；
Fig. 3 represents the B facial plane schematic diagrames of device in the utility model embodiment 1；
Fig. 4 represents the B faces schematic perspective view of device in the utility model embodiment 1；
Fig. 5 represents the A faces sealer welding figure of device in the utility model embodiment 1；
Fig. 6 represents the B faces sealer welding figure of device in the utility model embodiment 1；
Fig. 7 represents the A facial plane schematic diagrames of device in the utility model embodiment 2.
Reference：
1st, rotating shaft neck, 2, rotation fixed card slot, 3, adding mouth, the 4, first reagent chamber, the 5, first reagent flow road junction, 6,
First backflow gas port, the 7, second reagent chamber, the 8, second reagent flow road junction, the 9, second backflow gas road, the 10, the 3rd reagent chamber
Room, the 11, the 3rd reagent flow road junction, the 12, the 3rd backflow gas port, the reagent chamber of the 13, the 4th reagent chamber/the 5th, the 14, the 4th examination
Agent runner mouth, the 15, the 4th backflow gas port, 16, nucleic acid absorption chamber, 17, waste liquor stream road junction, 18, product stream road junction, 19, point
Analyse runner valve, 20, analysis backflow airway valve, 21, foranalysis of nucleic acids unit, 22, analysis runner, 23, nucleic acid PCR detection chambers, 24,
Reagent runner, the 25, first reagent runner valve, the 26, second reagent runner valve, the 27, the 3rd reagent runner valve, the 28, the 4th reagent flow
Road valve, 29, waste chamber, the 30, first reagent backflow air flue, the 31, first backflow airway valve, the 32, second backflow airway valve, 33,
3rd backflow airway valve, the 34, the 4th backflow airway valve, 35, product chamber, 36, product runner valve, 37, analysis runner mouth, 38,
Analysis backflow gas port, 39, matrix, the 40, second reagent backflow air flue, 41, analysis backflow air flue, 13', the 4th reagent chamber.
Embodiment
Below in conjunction with accompanying drawing of the present utility model, the technical scheme of the utility model embodiment is carried out clear, complete
Description.
A kind of nucleic acid extraction purification devices disclosed in the utility model, by the way that nucleic acid extraction is purified with analyzing multiple steps
Rapid required reagent, it is pre-packaged in a locking device；By nucleic acid extraction purifying and chamber and runner needed for analysis, it is integrated in
In the tow sides structure of integrated upright body disc type apparatus and in the radial direction reasonable layout；Applied in necessary instrument
Under external force (heating, centrifugal force caused by rotation, puncture) coordinates, nucleic acid extraction purifying (including liquid is realized in sequentially automation
Sample process, nucleic acid substances capture, nucleic acid substances purifying, nucleic acid substances elution) and follow-up detection and analysis (including nucleic acid production
Amplification, fluorescent PCR detection and the analysis of thing) process.
A kind of nucleic acid extraction purification devices disclosed in the utility model, it includes matrix 39, and is arranged on matrix 39
Reagent chamber, nucleic acid absorption chamber 16, waste chamber 29, product chamber 35 and foranalysis of nucleic acids unit 21, reagent chamber, waste liquid
Chamber 29 and product chamber 35 are communicated by corresponding runner with nucleic acid absorption chamber 16, and waste chamber 29 and product chamber 35 are logical
Corresponding backflow air flue is crossed to communicate with each reagent chamber.Matrix 39 is above-mentioned with certain thickness, vertical in the present embodiment 1
Body double-sided circular (shape is not limited to circle, can be other rule or irregular shapes, such as ellipse) disc type apparatus.Matrix
39 points are obverse and reverse, and above-mentioned each chamber, foranalysis of nucleic acids unit 21, runner, backflow air flue are located at two-sided disc type base respectively
The tow sides of body 39, or through with certain thickness matrix stereochemical structure；To reduce the volume of the utility model device, and
The distribution of each chamber, runner, backflow air flue etc. is set more to rationalize.
In order to facilitate description, the front of labeled substrate 39 is A faces, and reverse side is B faces.Fig. 1, Fig. 2 are represented in the present embodiment 1 respectively
The plan and stereogram in matrix A faces；Fig. 3, Fig. 4 represent the plan and stereogram in matrix B faces in the present embodiment 1 respectively.Its
In, a rotating shaft neck 1, multiple reagent chambers and foranalysis of nucleic acids unit 21 are arranged on matrix A faces；Waste chamber 29 and product
Chamber 35 is arranged on matrix B faces；Two rotation fixed card slots 2 and nucleic acid absorption chamber 16 run through matrix tow sides.
Wherein, rotating shaft neck 1 is used to drive whole device to rotate, and it is located at the circle centre position of matrix 39 and with the circle of matrix 39
Centered on the heart, the overall circular hole for having scarce plane in one.A faces and B faces that fixed card slot 2 runs through matrix 39 are rotated, it is arranged on
Set on the outside of reagent chamber and close to the outer circumferential edge of matrix 39, overall circular poroid in one, two rotation fixed card slots 2 are relative to be set
Put, respectively positioned at the both sides of rotating shaft neck 1.During concrete operations, device is fixed on by rotating shaft neck 1, rotation fixed card slot 2
In necessary instrument, and under outside mechanical force, drive disc type apparatus unidirectional or crankmotion.Rotation caused by from
Liquid flowing, transfer and mixing in mental and physical efforts drive device, to realize whole flows of nucleic acid extraction purification process, and will be final
Nucleic acid extraction product is applied to the detection of nucleic acids of follow-up a variety of purposes.In the present embodiment 1, the rotating speed of disc type apparatus rotation is big
Small is 2000rpm~10000rpm, and adjusting the speed of rotating speed can control liquid that nucleic acid absorption chamber is flowed and flowed through in runner
The speed speed of room.In addition, the device can rotate back and forth under the control of mechanical external force, make the liquid in each chamber fully mixed
It is even；Or the sorbing material of nucleic acid absorption intracavitary is set fully to be contacted with nucleic acid substances.It should be noted that the shape of rotating shaft neck,
Rotate fixed card slot position and shape be not limited to it is as described herein, as long as rotate fixed card slot set location realize will dress
Put fixed on relevant device.
Multiple reagent chambers are distributed on the position nearer apart from the center of circle of matrix 39, and each reagent chamber is distributed in matrix and turned
The outside of axle neck 1, and along the circumference of rotating shaft neck 1 but be not limited to it is circumferentially distributed, for store nucleic acid extraction and purifying needed for try
Agent.The utility model device typically sets three to five reagent chambers, and four reagent chambers are provided with the present embodiment 1, respectively
It is the first reagent chamber 4, the second reagent chamber 7, the 3rd reagent chamber 10 and the 4th reagent chamber 13, in the first reagent chamber 4
It is pre-packaged to have cell pyrolysis liquid and be reserved with the space needed for for adding liquid sample, the first reagent chamber 4 in the present embodiment 1
Can the volume of adding liquid sample be 100ul~500ul, liquid sample volume can according to sample properties, extraction product requirements amount,
And downstream is tested demand and increased and decreased.The adding mouth 3 for adding sample is additionally provided with first reagent chamber 4, passes through the sample-adding
In mouthful 3 adding liquid samples to the first reagent chamber 4, in the first reagent chamber 4, by the liquid sample that adding mouth 3 adds with
After pre-packaged cell pyrolysis liquid fully mixes, the cell in sample ruptures and discharged under the cooperation of external heat to be contained into the cell
Thing (material such as nucleic acid, albumen, polysaccharide), the mixing liquid flow through nucleic acid absorption chamber 16, and the nucleic acid substances in sample will be caught
Obtain in nucleic acid absorption chamber 16, liquid waste flows into waste chamber 29 by corresponding runner.
The pre-packaged cleaning solution having needed for washing step in second, third reagent chamber 7,10, cleaning solution flow through core successively
Acid absorption chamber 16, for washing in nucleic acid absorption chamber 16 in addition to nucleic acid, the impurity such as protein, polysaccharide, is discarded
Liquid flows into waste chamber 29 by corresponding runner.4th reagent chamber 13 is pre-packaged to be had nucleic acid substances from nucleic acid absorption chamber 16
The nucleic acid eluents of middle elution, after nucleic acid eluents flow through nucleic acid absorption chamber 16, capture and wash in nucleic acid absorption chamber 16
Nucleic acid substances after washing, release is formed into nucleic acid extraction purified product in solution is eluted, and product chamber is flowed into by corresponding runner
Room 35.Wherein, the quantity of washing step can be according to sample properties, extraction with the reagent chamber quantity of corresponding encapsulation cleaning solution
Time and downstream are tested demand and increased and decreased.The reagent chamber of one encapsulation cleaning solution can be such as set, and such reagent chamber is just
It is three；The reagent chamber of two encapsulation cleaning solutions can also be set, and such reagent chamber is exactly four, by that analogy.
Be provided with reagent flow road junction and the gas port that flows back corresponding with reagent flow road junction in each reagent chamber, i.e., first
The first reagent flow road junction 5 and the first backflow gas port 6 are provided with reagent chamber 4, the second examination is provided with the second reagent chamber 7
Agent runner mouth 8 and second flows back gas port 9, is provided with the 3rd reagent flow road junction 11 and the 3rd backflow gas in the 3rd reagent chamber 10
Road junction 12, the 4th reagent flow road junction 14 and the 4th backflow gas port 15 are provided with the 4th reagent chamber 13.Each reagent chamber
It is connected by respective reagent flow road junction with nucleic acid absorption chamber 16, and passes through respective backflow gas port and waste chamber 29
It is connected with product chamber 35.In the present embodiment 1, the reagent flow road junction in each reagent chamber is close to the outside of reagent chamber
Wall is set, and to be formed by be outwards the recessed semicircle arcuation side groove and being recessed downwards from chamber bottom surface of formation of the lateral wall from chamber
Circular groove composition.The madial wall that backflow gas port in each reagent chamber is close to reagent chamber is set, and is certainly
Chamber bottom surface is recessed downwards the circular groove of formation.Certainly, the shape of the reagent flow road junction in reagent chamber and backflow gas port
It is not limited to as described herein.
Nucleic acid absorption chamber 16 is through matrix tow sides and on the outside of reagent chamber, for capturing in liquid sample
Nucleic acid substances.Include comprising the above-mentioned sorbing material for being used to capture nucleic acid substances, the sorbing material in nucleic acid absorption chamber 16 but
It is not limited to the sorbing materials such as glass fibre, Silicon moulds or glass microballoon.Sorbing material (can such as add in corresponding reagent and external force
Heat and Nucleic Acid Elution reagent) coordinate under, by the nucleic acid substances captured release in cushioning liquid, with realize nucleic acid extraction purify
Purpose.
Nucleic acid absorption chamber 16 is connected by reagent runner 24 with each reagent chamber, reagent runner 24 and each chamber
Reagent flow road junction be connected, i.e., first, second, third, fourth reagent flow road junction 5,8,11,14 is connected with reagent runner 24
It is logical, so as to realize being connected for each reagent chamber and nucleic acid absorption chamber 16.When it is implemented, each reagent chamber can lead to
A public reagent runner 24 or being connected by respective reagent runner 24 and nucleic acid absorption chamber 16 are spent, that is,
Say that the lysate in reagent chamber, cleaning solution and eluent reagent can flow through nucleic acid absorption chamber successively by public reagent runner 24
Room 16.In the present embodiment 1, reagent runner 24 is distributed in the B faces of matrix, and is public.
The outside of nucleic acid absorption chamber 16 is additionally provided with waste liquor stream road junction 17 and product stream road junction 18, in the present embodiment 1, core
Acid absorption chamber 16, waste liquor stream road junction 17, product stream road junction 18 are located in same groove.Waste liquor stream road junction 17 connects nucleic acid absorption
Chamber 16 and waste chamber 29, caused liquid waste passes through the waste liquid runner under the influence of centrifugal force in each reagent chamber
Mouth 17 flows into waste chambers 29；Product stream road junction 18 connects nucleic acid absorption chamber 16 and product chamber 35, nucleic acid absorption chamber 16
Inside it is released the nucleic acid product to be formed and product chamber 35 is flowed into by the product stream road junction 18 under the influence of centrifugal force.
Waste chamber 29 and product chamber 35 are distributed in the outside of nucleic acid absorption chamber 16 on the radial direction of matrix
And be connected by corresponding runner with nucleic acid absorption chamber 16, it is respectively used to liquid waste after collecting reaction and is carried with nucleic acid is collected
Take purified product.Wherein, waste chamber 29 is interconnected by waste liquid runner (not shown) and nucleic acid absorption chamber 16, and nucleic acid is inhaled
The waste liquid flowed out in attached chamber 16 flows into waste chamber 29 by waste liquid runner；Specifically, waste chamber 29 and waste liquid runner phase
Connection, waste liquid runner are connected with waste liquor stream road junction 17, and waste liquor stream road junction 17 is connected with nucleic acid absorption chamber 16, so as to realize
The connection of waste chamber 29 and nucleic acid absorption chamber 16.By product runner, (figure is not with nucleic acid absorption chamber 16 for product chamber 35
Show) it is interconnected, the nucleic acid extraction purified product flowed out in nucleic acid absorption chamber 16 flows into product chamber 35 by product runner；Tool
For body, product chamber 35 is connected with product runner, and product runner is connected with product stream road junction 18, product stream road junction 18 with
Nucleic acid absorption chamber 16 is connected, it is achieved thereby that the connection of product chamber 35 and nucleic acid absorption chamber 16.
In addition, in order to control and ensure that liquid flows in locking device, waste chamber 29 and product chamber 35 are by right
The backflow air flue answered is connected with each reagent chamber.In the present embodiment 1, waste chamber by the first reagent flow back air flue 30 with
First reagent chamber 4, the second reagent chamber 7 are connected with the 3rd reagent chamber 10, specifically, the first reagent backflow air flue 30
It is connected with the backflow gas port 6,9,12 in first, second, and third reagent chamber 4,7,10, so as to realize waste chamber
29 are connected with first, second, and third reagent chamber 4,7,10；Product chamber 35 by the second reagent flow back air flue 40 with
4th reagent chamber 13 is connected, specifically, the 4th backflow in the second reagent backflow reagent chamber 13 of air flue 40 and the 4th
Gas port 15 is connected, so as to realize being connected for product chamber 35 and the 4th reagent chamber 13.Also set up on product chamber 35
There is analysis runner mouth 37, analysis is provided with the second reagent backflow air flue 40 that product chamber 35 connects with the 4th reagent chamber 13
Flow back gas port 38.
Preferably, in order to control the reagent in each reagent chamber to flow through nucleic acid absorption chamber 16 in a certain order,
In the reagent runner 24 that each reagent chamber is connected with nucleic acid absorption chamber 16, corresponding each reagent chamber is provided with accordingly
Runner valve, respectively the first reagent runner valve 25, the second reagent runner valve 26, the 3rd reagent runner valve 27 and the 4th reagent flow
Road valve 28, respective valves are opened successively according to nucleic acid extraction purifying flow, to ensure that different reagents flow successively according to certain order
Through nucleic acid absorption chamber 16, i.e. the first reagent runner valve 25, the second reagent runner valve 26, the 3rd reagent runner valve 27 and the 4th examination
Agent runner valve 28 is opened successively according to sequencing, so that the liquid in corresponding reagent chamber flows through nucleic acid absorption chamber successively
16。
Preferably, it is also equipped with the backflow air flue that each reagent chamber is connected with waste chamber 29, product chamber 35
The return valve corresponding with the runner valve in reagent chamber.In the present embodiment 1, waste chamber 29 and first, second and
In the first reagent backflow air flue 30 that 3rd reagent chamber 4,7,10 is connected, corresponding first, second, and third reagent chamber 4,
7th, 10 corresponding return valve, the respectively first backflow backflow backflow gas of airway valve 32 and the 3rd of airway valve 31, second are provided with
Road valve 33；In the second reagent backflow air flue 40 that product chamber 35 is connected with each reagent chamber, corresponding 4th reagent chamber
13 are provided with corresponding return valve, for the 4th backflow airway valve 34.
Preferably, in the product runner that nucleic acid absorption chamber 16 is connected with product chamber 35, it is provided with and the 4th backflow gas
The corresponding product runner valve 36 of road valve 34；It is also equipped with the waste liquid runner that waste chamber 29 connects with nucleic acid absorption chamber 16
Waste liquid runner valve (not shown), waste liquid runner valve can be opened under outer power cooperation, make the cell pyrolysis liquid discarded after reaction and wash
Wash liquid and waste chamber 29 is flowed into by waste liquid runner；The valve can be again switched off under outer power cooperation, to ensure in waste chamber 29
Waste liquid no longer flow out, while there is no other liquid flow into waste chamber 29.
Foranalysis of nucleic acids unit 21 is radially disposed in the outside of product chamber 35 in matrix, and is connected with product chamber 35, product
Nucleic acid extraction purified product in chamber 35 flows into foranalysis of nucleic acids unit 21 by corresponding runner, analyzes and examines for nucleic acid product
Survey.Specifically, foranalysis of nucleic acids unit 21 is connected with the analysis runner mouth 37 on product chamber 35, so as to connect foranalysis of nucleic acids list
Member 21 and product chamber 35.Foranalysis of nucleic acids unit 21 is also connected by analyzing backflow air flue 41 with product chamber 35, specifically,
Analysis backflow air flue 41 is connected with the analysis backflow gas port 38 on the second reagent backflow air flue 40.Foranalysis of nucleic acids unit 21 exists
Under the cooperation of external hot circulation and optical detecting module, the detection work(such as nucleic acid PCR amplification, real-time fluorescence detection can realized
Energy.
It should be noted that liquid is at runner (reagent runner 24, waste liquid runner, product runner, analysis runner 22 or 22')
In interior flow process, the corresponding backflow air flue of this runner (the first reagent backflow air flue 30, the second reagent backflow air flue
40th, analysis backflow air flue 41) in respective valves will be all turned on, to ensure the air pressure balance in locking device, enable liquid
It is enough to be smoothly transferred to another chamber from a chamber.Before reagent runner being flowed into such as the mixing liquid in the first reagent chamber 4,
The first reagent runner valve 25 and the first backflow airway valve 32 are opened simultaneously, and the second~the 4th reagent chamber is similar；And for example product chamber
Nucleic acid product in room 35 enters before foranalysis of nucleic acids unit 21, need to open analysis runner valve 19, analysis backflow airway valve simultaneously
20.Valve in runner inner flow passage valve and backflow air flue coordinates unlatching/closing, can control and ensure liquid in locking device
Interior flowing.In addition, before using the utility model device, set valve will close in all runners, backflow air flue, pass through
After the adding liquid sample of adding mouth 3, adding mouth 3 is sealed by sealing.
Preferably, runner, valve set in backflow air flue can be puncture valve, and puncture valve (is schemed in outside pressing structure
Do not show) in the presence of will be punctured, the runner, backflow air flue will be switched on.Outside pressing structure can be with one stream of a secondary control
Road, flow back air flue conducting, the multiple runners of a secondary control that can also combine, backflow air flue while turn on and closure.
In the embodiment of some replacements, set valve can be heat-sealing valve in the runner of the utility model device, the air flue that flows back, hot
Envelope valve will be opened in the presence of external heat, is heated again, will be again switched off the valve.In the utility model embodiment 1
What the valve of setting was selected is puncture valve.
Preferably, nucleic acid extraction of the present utility model purifies dimensional double-sided disc type apparatus by least one plastics, resinous wood
Material is made, including polyethylene, low density polyethylene (LDPE), polypropylene, polyvinyl chloride etc..The chamber that the utility model device is provided
(each reagent chamber, waste chamber, product chamber) can be by least one layer of plastic film material plastic packaging in rigid plastics material
Upper formation, it can also be sealed and be formed by the membranaceous plastic material of at least two layers of thin, the construction of chamber may include various shapes, such as fan
Shape, circle, ellipse, water-drop-shaped or irregular shape.
Fig. 5, Fig. 6 represent the utility model device A faces and B faces sealer welding figure.Chamber included by the utility model device
Room, runner, backflow air flue, foranalysis of nucleic acids unit and neck, are on certain thickness disc type rigid plastics, two-sided carving
Carve, three-dimension process, and the welding of rigid plastics upper sealing film is covered in by plastic film material and completed, the grey area in Fig. 5, Fig. 6
Domain representation the utility model device needs the region welded during the manufacturing.
Specifically, in some embodiments, the utility model device is provided runner (reagent runner, product runner,
Waste liquid runner) it can be formed by least one layer of plastic film material plastic packaging in rigid plastics material, can also be by least two layers
Film-form plastic material seals to be formed, and the construction of runner may include various shapes, such as linear pattern, circular arc, or irregular shape.
In some embodiments, the utility model device is integrally with certain thickness dual-surface stereo disc type knot
Structure, to meet the reasonable layout in device tow sides of multiple chambers and runner.
In some embodiments, the utility model device can be relatively thin single layer structure in subregion, to adapt to
The rapid temperature rise and drop demand of reaction in some chambers.
It should be noted that the chamber on the utility model device, according to its position in the radial direction, in centrifugal force
Driving under, be one-way flow in the runner that liquid connects between chamber.And in some embodiments, the utility model institute
The cracking of the nucleic acid extraction purification step being related to, such as cell, Nucleic Acid Elution react, and foranalysis of nucleic acids detection reaction needs to add in outside
Realized under the cooperation of heat.
In addition, in summary, the liquid mixing involved by the utility model, such as the mixing of sample and reagent；Nucleic acid extraction
The mixing of purified product；Mixing of nucleic acid extraction purified product and detection reagent etc. in outside modular belt, it is necessary to move described device
Completed under the cooperation rotated back and forth.Liquid transfer involved by the utility model is unidirectionally revolved, it is necessary to move device in outside modular belt
Completed under the cooperation turned.The regulation of rotating speed size, flow velocity of the controllable liquid in runner, liquid flow through nucleic acid absorption chamber
Speed.Runner conducting involved by the utility model, if the unlatching of puncture valve under the cooperation of outside pressing, it is necessary to complete；This
Runner closure involved by utility model, the closing of valve is such as sealed, it is necessary to be completed under the cooperation of external heat.The utility model
The runner of device is only located at the one side of dual-surface stereo tray type structure, and two chambers of the connection in device the same face, liquid is flat
Flowed from inside to outside along radial direction in the structure of face.Or the runner of the utility model device can cross over there is certain thickness
Dual-surface stereo tray type structure, connection in device tow sides two chambers, liquid is in dual-surface stereo structure along radius
Flow from inside to outside in direction.
The liquid flow patterns of the utility model device are realized by centrifugal force and driven, and coordinate valve in runner, backflow air flue
Control, make different liquids in a device according to different order, successively directed flow.Operation principle of the present utility model is：Liquid
Body sample under the action of the centrifugal force, by the chamber (such as each reagent chamber) nearer apart from the center of circle, flows through nucleic acid absorption chamber, stream
To the chamber (such as waste chamber and product chamber) apart from the center of circle farther out；In the process, the nucleic acid substances in sample are in core
It is captured in sour adsorbent chamber room, repeatedly washs, product chamber is flow to after elution, nucleic acid extraction purge process is completed, finally in core
Subsequent detection and analysis are carried out in acid analysis unit.Specifically, the idiographic flow of nucleic acid extraction purifying of the present utility model includes：
The utility model device B is put into necessary instrument upwardly, incite somebody to action this by rotating shaft neck 1 and rotation fixed card slot 2
Utility model device is fixed on the relevant position in necessary instrument.After device B faces are by the adding liquid sample of adding mouth 3, dress
Closing will be heat-sealed by putting the adding mouth 3 in B faces.
Liquid sample after addition enters the first reagent chamber 4, rotates back and forth and fills under outer power cooperation with cell pyrolysis liquid
Divide and mix, and will by the material such as the cell rupture in sample, intracellular nucleic acid, albumen, polysaccharide under conditions of external heat
It is released.
Pressed by external force punctures the first reagent runner puncture valve 25, first backflow air flue puncture valve 31, in the first reagent chamber 4
Mixing liquid reagent runner 24 will be entered by the first reagent flow road junction 5 under the influence of centrifugal force, and flowed along reagent runner 24
Enter nucleic acid absorption chamber 16, nucleic acid substances will be trapped in nucleic acid absorption chamber 16, and liquid waste is by the effect of centrifugal force
Under by waste streams road junction 17, flow into waste chamber 29.
Pressed by external force punctures the second reagent runner puncture valve 26, second backflow air flue puncture valve 32, in the second reagent chamber 7
Cleaning solution 1 reagent runner 24 will will be entered by the second reagent flow road junction 8 under the influence of centrifugal force, and flowed along reagent runner 24
Enter nucleic acid absorption chamber 16, wash other impurity in addition to nucleic acid, liquid waste will be under the influence of centrifugal force by waste streams
Road junction 17, flow into waste chamber 29.
Pressed by external force punctures the 3rd reagent runner puncture valve the 27, the 3rd backflow air flue puncture valve 33, the 3rd reagent chamber 10
Interior cleaning solution 2 will will enter reagent runner 24 by the 3rd reagent flow road junction 11 under the influence of centrifugal force, and along reagent runner
24 flow into nucleic acid absorption chamber 16, wash other impurity in addition to nucleic acid, liquid waste will be under the influence of centrifugal force by giving up
Abandoned stream road junction 17, flow into waste chamber 29.
The waste streams road junction 17 in device B faces will be heat-sealed closing, and the nucleic acid eluents in the 4th reagent chamber 13 are in outside
It is preheated under conditions of heating.
Pressed by external force punctures the 4th reagent runner puncture valve the 28, the 4th backflow air flue puncture valve 34, product runner puncture valve
36, the nucleic acid eluents being preheated in the 4th reagent chamber 13 will be entered by the 4th reagent flow road junction 14 under the action of the centrifugal force
Enter reagent runner 24, and nucleic acid absorption chamber 16 is flowed into along reagent runner 24, the nucleic acid substances captured will be released to form core
Acid product, nucleic acid product will flow into product chamber 35 under the action of the centrifugal force by product stream road junction 18.
Nucleic acid product into product chamber 35 will rotate back and forth abundant mixing under outer power cooperation, will be transferred afterwards
To foranalysis of nucleic acids unit 21.Foranalysis of nucleic acids unit 21 realizes that nucleic acid produces under the cooperation of external hot circulation and optical detecting module
Amplification, fluorescent PCR detection and the analysis of thing.
Embodiment 2：
Fig. 7 represents the A facial plane schematic diagrames of the embodiment 2 of the utility model device, and the embodiment and the device of implementation column 1 are not
With the embodiment includes five reagent chambers, is respectively used to store the first reagent chamber 4 of cell pyrolysis liquid, is used for
Store the second reagent chamber 7 of cleaning solution 1, the 3rd reagent chamber 10 for storing cleaning solution 2, for storing cleaning solution 3
4th reagent chamber 13', the 5th reagent chamber 13 for storing nucleic acid eluents.The cleaning solution chamber of the utility model device
Quantity can according to sample properties, extraction time and downstream test demand and increase and decrease.
Nucleic acid extraction purification devices provided by the utility model, are applicable to plurality of liquid sample, including but not limited to raw
Thing sample, or the environmental samples containing biological substance, solvent sample etc..In some embodiments, the utility model is applicable
Sample can be liquid bio sample, such as body fluid (blood, saliva, urine, lymph, cerebrospinal fluid, seminal fluid, bile), group
Knit liquid, intracellular fluid etc..In some embodiments, the sample that the utility model is applicable can be containing the molten of biological substance
Agent sample, as swab preserves liquid, microbial culture medium, excrement dilution, nasal cavity, vagina cleaning solution, tissue grinder liquid etc..At certain
In a little embodiments, the sample that the utility model is applicable can be the liquid environment sample for including biological substance, such as seawater,
River, lake water, well water, soil liquid etc..
Technology contents and technical characteristic of the present utility model are had revealed that as above, but those skilled in the art still may be used
A variety of replacements and modification without departing substantially from the utility model spirit can be made based on teaching of the present utility model and announcement, therefore, this
Utility model protection scope should be not limited to the content disclosed in embodiment, and should include various without departing substantially from replacement of the present utility model
And modification, and covered by present patent application claim.
Claims (10)
1. a kind of nucleic acid extraction purification devices, it is characterised in that it includes：Matrix, and the multiple reagent chambers being arranged on matrix
Room, nucleic acid absorption chamber, waste chamber, product chamber and foranalysis of nucleic acids unit, the reagent chamber, waste chamber and product chamber
Room is communicated by corresponding runner with nucleic acid absorption chamber, waste chamber and the product chamber by flow back accordingly air flue with
Each reagent chamber communicates, and each chamber, runner and backflow air flue are located at the front of matrix, reverse side or through matrix, the examination
Agent chamber is set close to the center of matrix；And the reagent chamber, nucleic acid absorption chamber, waste chamber and foranalysis of nucleic acids list
Member, or the reagent chamber, nucleic acid absorption chamber, product chamber and foranalysis of nucleic acids unit matrix radial direction from inside to outside according to
Secondary setting, the foranalysis of nucleic acids unit are connected with product chamber；Fluid sample rotated in matrix caused by under centrifugal action,
Successively by reagent chamber, nucleic acid absorption chamber is flowed through, and flows to waste chamber and product chamber respectively, completes nucleic acid extraction purifying
Process, subsequent detection and analysis are finally carried out in foranalysis of nucleic acids unit.
2. nucleic acid extraction purification devices according to claim 1, it is characterised in that the reagent chamber includes the first reagent
Chamber, at least one second reagent chamber and the 3rd reagent chamber, it is provided with for adding liquid in first reagent chamber
The adding mouth of sample, and pre-packaged have cell pyrolysis liquid and be reserved with the space needed for for adding liquid sample；Described second
Reagent chamber is pre-packaged cleaning solution；3rd reagent chamber is pre-packaged to be had and elutes nucleic acid substances from nucleic acid absorption chamber
Nucleic acid eluents.
3. nucleic acid extraction purification devices according to claim 1 or 2, it is characterised in that the reagent chamber passes through public
Or be connected by respective reagent runner with nucleic acid absorption chamber；The waste chamber passes through waste liquid runner and nucleic acid absorption
Chamber is connected；The product chamber is connected by product runner with nucleic acid absorption chamber；The waste chamber also passes through
One reagent backflow air flue is connected with reagent chamber；The product chamber also passes through the second reagent backflow air flue and reagent chamber phase
Connection；The foranalysis of nucleic acids unit is connected by analyzing backflow air flue with product chamber.
4. nucleic acid extraction purification devices according to claim 3, it is characterised in that corresponding each examination in the reagent runner
Agent chamber is provided with reagent runner valve, is provided with the first reagent backflow air flue and the second reagent the backflow air flue and reagent flow
The corresponding backflow airway valve of road valve.
5. nucleic acid extraction purification devices according to claim 4, it is characterised in that the reagent runner in the reagent runner
Valve is opened successively, different reagents is sequentially flowed through nucleic acid absorption chamber successively；While reagent runner valve is opened, the first reagent returns
The backflow airway valve corresponding with reagent runner valve is opened simultaneously in gas road or the second reagent backflow air flue.
6. nucleic acid extraction purification devices according to claim 3, it is characterised in that the reagent chamber, foranalysis of nucleic acids list
Member, analysis backflow air flue are distributed in wherein on end face of matrix；The reagent runner, waste chamber, product chamber, the first examination
Agent backflow air flue, the second reagent backflow air flue are distributed on the other end of matrix；The nucleic acid absorption chamber runs through matrix.
7. nucleic acid extraction purification devices according to claim 1, it is characterised in that described matrix passes through for fixing device
And the rotary fixing structure of driving device rotation is fixed in necessary instrument, necessary instrument drives under outside mechanical force
Matrix is unidirectional or crankmotion.
8. nucleic acid extraction purification devices according to claim 7, it is characterised in that the rotary fixing structure includes setting
At least one rotating shaft neck and/or at least one rotation fixed card slot on matrix, the rotating shaft neck are located in matrix
It is arranged concentrically at the heart and with matrix, the rotation fixed card slot is arranged on the outside of reagent chamber and set close to the outer side edges of matrix
Put, the rotation fixed card slot runs through matrix.
9. nucleic acid extraction purification devices according to claim 1, it is characterised in that the reagent chamber, nucleic acid absorption chamber
Room, waste chamber, product chamber, runner are formed by least one layer of plastic film material plastic packaging in rigid plastics material, or by
At least membranaceous plastic material of two layers of thin seals to be formed.
10. nucleic acid extraction purification devices according to claim 1, it is characterised in that described matrix generally has certain
Thickness, two-sided processing, three-dimensional tray type structure, it is the single layer structure that thickness is less than other regions in subregion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720582714.7U CN206814737U (en) | 2017-05-24 | 2017-05-24 | A kind of nucleic acid extraction purification devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720582714.7U CN206814737U (en) | 2017-05-24 | 2017-05-24 | A kind of nucleic acid extraction purification devices |
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| CN206814737U true CN206814737U (en) | 2017-12-29 |
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| CN201720582714.7U Active CN206814737U (en) | 2017-05-24 | 2017-05-24 | A kind of nucleic acid extraction purification devices |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018214907A1 (en) * | 2017-05-24 | 2018-11-29 | 西安天隆科技有限公司 | Device and method for nucleic acid extraction and purification |
| CN113009136A (en) * | 2020-08-21 | 2021-06-22 | 东莞东阳光医疗智能器件研发有限公司 | Small-sized multi-index detection sample analysis device |
| CN113528287A (en) * | 2020-04-16 | 2021-10-22 | 广东润鹏生物技术有限公司 | Sample processing device and PCR instrument |
-
2017
- 2017-05-24 CN CN201720582714.7U patent/CN206814737U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018214907A1 (en) * | 2017-05-24 | 2018-11-29 | 西安天隆科技有限公司 | Device and method for nucleic acid extraction and purification |
| CN113528287A (en) * | 2020-04-16 | 2021-10-22 | 广东润鹏生物技术有限公司 | Sample processing device and PCR instrument |
| CN113528287B (en) * | 2020-04-16 | 2022-12-23 | 广东润鹏生物技术有限公司 | Sample processing device and PCR instrument |
| CN113009136A (en) * | 2020-08-21 | 2021-06-22 | 东莞东阳光医疗智能器件研发有限公司 | Small-sized multi-index detection sample analysis device |
| CN113009136B (en) * | 2020-08-21 | 2024-04-05 | 东莞东阳光医疗智能器件研发有限公司 | Small multi-index detection sample analysis device |
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