CN207537439U - Fully automatic integral nucleic acid extraction, amplification and detecting system - Google Patents
Fully automatic integral nucleic acid extraction, amplification and detecting system Download PDFInfo
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- CN207537439U CN207537439U CN201721483493.4U CN201721483493U CN207537439U CN 207537439 U CN207537439 U CN 207537439U CN 201721483493 U CN201721483493 U CN 201721483493U CN 207537439 U CN207537439 U CN 207537439U
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Abstract
The utility model discloses fully automatic integral nucleic acid extraction, amplification and detecting system, which coordinates disposable, the closed two-sided disc type reaction box of pre-packaged reagent, realizes the Automatic nucleic acid Testing of " sample into as a result go out ".The system includes the drive control microscope carrier and a top control assembly acted on from top same reaction box application for loading, rotating and acted on from bottom to two-sided disc type reaction box application.The performer for acting perpendicularly to two-sided disc type reaction box is installed in drive control microscope carrier and top control assembly, these performers offer reaction box is fixed and rotational positioning, reaction box is unidirectional or reciprocating rotary, high speed centrifugation driving liquid flowing, the opening or closing of the switches such as adding mouth, valve and runner or diaphragm seal on reaction box, the heating of pre-packaged reagent and the absorption of magnetic bead, the experimental implementations such as multi-zone temperature control and multichannel fluoroscopic examination, realize fully automatic integral nucleic acid extraction, amplification and detection in closed reaction box.
Description
Technical field
The utility model belongs to biomedical gene analytical instrument field more particularly to one kind for fully automatic integral core
Acid extraction, amplification and detecting system.
Background technology
GENE Assay analysis is the basic experiment method of modern biomedical.Molecule diagnosis is applied molecular biology method
Detection patient's body is exogenous, the variation of structure of own genetic material or expression and make the technology of diagnosis, be the present age
One of important front edge field of medical development.The basis of molecule diagnosis is the analysis for target nucleic acid, and routine techniques includes：
Fluorescent PCR, fluorescence in situ hybridization (FISH), DNA sequencing, southern blotting technique, single nucleotide polymorphism (SNP), ligase chain reaction
(LCR) and genetic chip etc. it, regardless of being that these target nucleic acids are carried out with which kind of operation and detection, is required for it from biology
It extracted in sample (blood, saliva, sperm or other secretion), be purified and can carry out.Therefore, the effect of nucleic acid extraction
The height of rate and purity will directly affect the accuracy of the development and experimental result of later stage research work.
In addition, all molecular diagnostic techniques will continue to optimize signal-to-noise ratio, this just need by from sample extraction purification go out
The target nucleic acid sequence come carries out specific amplification, and amplification target mrna signal is detected, so as to meet needed for molecule diagnosis
Specificity and sensitivity.
Demand of the clinical examination to molecule diagnostic instrments is many and complicated various, including the following：
First, the product fast and automatically changed.Due to the clinical detection that face enormous amount daily, even receiving
The laboratory operation personnel of special training, in face of complicated sample process, nucleic acid extraction, purifying and amplification step, it is also difficult to keep away
Exempt from the manual mistake in operating process.Therefore, simplify manual operation, shorten detection cycle, realize the automatic of molecule diagnostic instrments
Change, be the emphasis for improving diagnosis efficiency, reducing false diagnosis.
Second, totally-enclosed, free of contamination product.Many molecule diagnostic instrments are needed in the extraction for completing target nucleic acid at present
And after purification, product is transferred to progress next step reaction on amplification instrument；Also some methods are needed after amplification, by amplified production
Transfer is detected.These transfer operations easily cause the pollution in laboratory, and many basic hospitals, do not have foundation mark
Quasi-molecule diagnostic test room condition (by lab setup into it is multiple for samples extract, reaction establish, amplification and detection every
From room).Therefore, totally enclosed molecular diagnostic system is to avoid pollution optimal path.
Third, integrated, miniaturization product.Molecule diagnosis is made of, and each step various and complicated step
It is required for different reagents and operation.This so that most of molecule diagnosis are required in laboratory at present, larger by volume
Instrument is completed, and greatly limits the application range of molecule diagnosis.If it is possible to by needed for nucleic acid extraction, amplification and detection
Whole reagents it is pre-packaged in a disposable, closed reaction box；After sample to be tested is added in, the reaction box is small at one
Realize that nucleic acid extraction, amplification and detection Full automatic closed operation, all operationss needed for process are carried by instrument in type instrument
It is completed for external action condition, molecule diagnosis is made to be no longer limited by the conditions such as personnel's operation, reagent and laboratory, this will expand significantly
Open up the application range of molecular diagnostic techniques.
Patent of invention application No. is 201710371949.6 discloses a kind of nucleic acid extraction purification devices, it is a kind of incites somebody to action
Nucleic acid extraction, amplification and the pre-packaged closed two-sided disc type reaction box of detection reagent.Realizing has the characteristics that aforementioned three kinds
Molecule diagnostic products also need exploitation one kind that can provide nucleic acid extraction, amplification and external action behaviour needed for detection fully automatic operation
The integrated instrument of work realizes the nucleic acid detection assay of " sample into as a result go out ".Reduce equipment instrument simultaneously, shorten whole process
Time is the active demand in market.
Invention content
The purpose of this utility model is to provide a kind of fully automatic integral nucleic acid extraction, amplification and detecting systems, this is
Disposable, the closed two-sided disc type reaction box under unified central planning for closing pre-packaged reagent realizes the full-automatic nucleic acid of " sample into as a result go out "
Detection and analysis.
To achieve these goals, the utility model provides a kind of integrated nucleic acid extraction, amplification and detecting system,
It is characterized in that, drive control microscope carrier including top control assembly, below the control assembly of top and is pushed up for adjusting
The connection component of relative position between portion's control assembly and drive control microscope carrier；
Multiple performers are provided in the top control assembly and drive control microscope carrier, the performer includes electricity
Thermomechanical components hold down assembly, temperature control component, switching device, detection components and drive component；Wherein：
The electric machine assembly includes the electric rotating machine being located on drive control microscope carrier, for the rotation of reaction box；
It is described to hold down assembly in the control assembly of top, for the compression of reaction box；The temperature control component includes two groups
Or multigroup PCR warm areas control unit, every group of PCR warm areas control unit include two and are mounted on top control assembly and driving relatively
The temperature control module on microscope carrier is controlled, the control temperature of two temperature control modules is identical, forms the temperature needed for PCR reactions in reaction box
Area, the control temperature of different PCR warm areas control units are different；
The switching device be used to open or capping box in various switches；
The detection that the detection components are reacted for PCR in reaction box；
The driving component at least there are two, be respectively arranged in top control assembly and drive control microscope carrier on, realize pressure
Tight component, temperature control component, heater element, switching device, the pushing for detecting device or upper lift.
In some embodiments, the connection component can be to move horizontally component, and the component that moves horizontally includes
Horizontally disposed leading screw driving structure and guide rail, the drive control microscope carrier are pushing up under the driving of leading screw driving structure along guide rail
It is translated between the underface and side of portion's control assembly.
In some embodiments, the connection component can be vertical motion assemblies, and the vertical motion assemblies include
Vertically disposed leading screw driving structure and guide rail, the top control assembly are close along guide rail under the driving of leading screw driving structure
Or far from drive control microscope carrier.
In some embodiments, the connection component can be overturning component, and the overturning component includes being located at top
Articulated structure and driving motor between control assembly and drive control microscope carrier；The top control assembly is in driving motor
It is overturn under driving around articulated structure.
Preferably, the electric machine assembly includes the electric rotating machine being located on drive control microscope carrier, the rotation of the electric rotating machine
Shaft is corresponding with the fixed card slot of two-sided disc type reaction box circle centre position, and reaction box can be fixed in the screens of rotary shaft.
Preferably, the electric rotating machine can drive two-sided disc type reaction box to carry out unidirectional or reciprocating rotary, make reaction box
It is located in drive control microscope carrier or top control assembly carries out effect desired position to it.
Preferably, the electric rotating machine is equipped with coding disk, can to the absolute position of two-sided disc type reaction box and rotating speed into
Row negative feedback control.The rotating speed control of the reaction box, rate curve can be triangular wave, sawtooth wave, sine wave or throwing
Object line etc., the frequency and amplitude of speed curves can be with dynamic regulations, and then realize in reaction box different volumes liquid in different cavity body
The rapidly and efficiently mixing of body.
Preferably, the electric rotating machine can drive two-sided disc type reaction box to carry out high speed single direction rotation, and rotating speed is reachable
2000~10000 revs/min.Under suitable rotating speed, no matter the runner between cavity and cavity is being distributed in reaction box just
Face or reverse side, the centrifugal force that the unidirectional high speed rotation of reaction box generates can drive liquid from the cavity near apart from reaction box center
The flow direction cavity remote apart from reaction box center；Rotating speed can be with dynamic regulation, to control flowing speed of the liquid in runner or cavity
Degree adjusts nucleic acid absorption, washing and the efficiency of elution when carrying out the purifying of column embrane method nucleic acid extraction, obtains best nucleic acid yield
And purity.
Preferably, described hold down assembly includes the compression leg of at least three circumferentially equidistantly distributeds, and system can control
Compression leg compresses downwards reaction box simultaneously, it is made to be fixed on position location.After reaction box is fixed by compression leg, drive control microscope carrier and top
Other performers of portion's control assembly can act on completing nucleic acid extraction, amplification and detection function to reaction box application.
After each driving element completes certain effect by flow, compression leg withdraws release reaction box and continues other effect flows upwards.
Preferably, the switching device can be used to open or the mandril or thorn of capping box inner valve
The heat-sealing heat-sealing weldering of adding mouth or runner diaphragm seal in the lancet or capping box of broken reaction box interior sealing film
Connector.
Preferably, the PCR reaction chambers of the reaction box can be driven by electric rotating machine, in two or more sets PCR warm area controls
It fast transfer and is clamped back and forth between unit processed, meets reverse transcription or the temperature requirement needed for PCR amplification, realize the amplification of nucleic acid
Reaction.
Preferably, the detection that the detection components are reacted for PCR in reaction box.Preferably, the detection components include
The multiple fibre-optical probes installed on the temperature control module of a PCR warm area control unit of top drive, the PCR warm area controls
The temperature of unit control processed is annealing extension step required temperature in PCR reaction process.Extend process in the annealing of PCR reactions
In, multiple fibre-optical probes carry out multichannel fluorescence excitation detection to PCR reactions multiple in two-sided disc type reaction box respectively, realize PCR
Real-time fluorescence detection in reaction process.
Preferably, the driving component can be used for two temperature control modules up and down of every group of warm area control unit is driven to clamp
The PCR reaction cavity regions of reaction box；
Preferably, the driving component includes rotational structure and cam structure；The rotational structure is used for cam structure
Turn to the driving end of the performer of implementation；The cam structure for drive corresponding performer push or
Lift.
Preferably, heater element and/or magnetic are also provided on the top control assembly and drive control microscope carrier
The performers such as device are used to implement column embrane method or paramagnetic particle method nucleic acid extraction flow.
Preferably, the heater element for during reaction box nucleic acid extraction purification lysate, eluent plus
Heat；
Preferably, the magnetic assembly is for the magnetic bead adsorption operations in paramagnetic particle method extraction process.
The beneficial effects of the utility model：
1st, the utility model is used for integrated nucleic acid extraction, amplification and detecting system, coordinate pre-packaged reagent it is disposable,
Enclosed two-sided disc type reaction box uses.System operatio is simple, easy to use, only need to be loaded directly into original sample, system can
It is right especially suitable for the Site Detection of non-lab environment automatically to realize the nucleic acid detection assay of " sample into as a result go out "
Operating personnel require without profession.
2nd, principle of the system of the utility model based on rotating centrifugal realizes sample pre-treatments and nucleic acid extraction purification process,
It is controlled in different cavity body fluid body blending process by the Frequency And Amplitude Modulation of speed curves, realizes rapidly and efficiently mixing；Liquid shifts
Process is by optimizing high-speed rotating rate, to control flowing velocity and time of the liquid in runner or cavity, is carrying out column
When embrane method nucleic acid extraction purifies, nucleic acid absorption, washing and the efficiency of elution are adjusted, is obtained most preferably in shortest run time
Nucleic acid yield and purity.
3rd, the system of the utility model is by controlling multiple PCR reaction chambers of two-sided disc type reaction box in different constant temperature
Rapid PCR amplification is realized in switching between warm area, without scanning when fibre-optical probe carries out multichannel fluoroscopic examination, so system is completed
The speed of real-time fluorescence PCR detection of nucleic acids is also very fast.Usual system complete the full-automatic nucleic acid extraction of a sample, amplification and
The time of detection is only 30~45 minutes.
4th, for the system of the utility model after two-sided disc type reaction box adds in sample, system seals plumb joint by heat-sealing will
Adding mouth sealed membrane is closed, and whole nucleic acid extraction, amplification and detection process are all completed inside reaction box later, good envelope
Closing property and disposable reaction box, can be to avoid the possibility polluted in experimentation.
5th, in the system of the utility model drive component by translation structure and cam structure, hold down assembly, temperature control group
Part, heater element, switching device, detection components etc. push or on lift work and warm area unit pinching action, by perpendicular to two-sided
The action of disc type reaction box is converted into the action for being parallel to reaction box so that system structure is compact simple, can substantially reduce instrument
The size of device makes it be more suitable for the Site Detection of non-laboratory condition.
Description of the drawings
Fig. 1 shows the A facial plane schematic diagrames of two-sided disc type reaction box；
Fig. 2 represents the A faces stereoscopic schematic diagram of two-sided disc type reaction box；
Fig. 3 represents the B facial plane schematic diagrames of two-sided disc type reaction box；
Fig. 4 represents the B faces stereoscopic schematic diagram of two-sided disc type reaction box；
Fig. 5 represents the A faces sealer welding figure of two-sided disc type reaction box；
Fig. 6 represents the B faces sealer welding figure of two-sided disc type reaction box；
Fig. 7 represents the overall schematic (loading position) of Tthe utility model system；
Fig. 8 shows the bottom views of Tthe utility model system drive control microscope carrier；
Fig. 9 represents the vertical view of control assembly at the top of Tthe utility model system；
Figure 10 a represent that Tthe utility model system temperature control component and detection components react warm area in the first PCR respectively with Figure 10 b
Two states schematic diagram when control unit works and when the 2nd PCR reaction warm area control units work；
Figure 11 represents the speed curves of Tthe utility model system electric rotating machine；
Figure 12 a represent a specific embodiment of drive component in control assembly at the top of Tthe utility model system；
Figure 12 b are the cam structure figures in Figure 12 a；
Figure 13 a, 13b, 13c seal three specific implementation row of plumb joint for Tthe utility model system.
Wherein reference numeral is：1st, shaft card slot, 2, rotation fixed card slot, 3, adding mouth, the 4, first reagent chamber, 5, the
One reagent flow road junction, the 6, first reflux gas port, the 7, second reagent chamber, the 8, second reagent flow road junction, the 9, second reflux gas
Road, 10, third reagent chamber, 11, third reagent flow road junction, 12, third reflux gas port, the examination of the 13, the 4th reagent chamber/5th
Agent chamber, the 14, the 4th reagent flow road junction, the 15, the 4th reflux gas port, 16, nucleic acid absorption chamber, 17, waste liquor stream road junction, 18,
Product stream road junction, 19, analysis runner valve, 20, analysis reflux airway valve, 21, foranalysis of nucleic acids unit, 22, analysis runner, 23, PCR
Reaction chamber, 24, reagent runner, the 25, first reagent runner valve, the 26, second reagent runner valve, 27, third reagent runner valve, 28,
4th reagent runner valve, 29, waste chamber, the 30, first reagent reflux air flue, the 31, first reflux airway valve, the 32, second reflux
Airway valve, 33, third reflux airway valve, the 34, the 4th reflux airway valve, 35, product chamber, 36, product runner valve, 37, analysis
Runner mouth, 38, analysis reflux gas port, 39, matrix, the 40, second reagent reflux air flue, 41, analysis reflux air flue；1a, instrument
Pedestal, 2a, instrument left side plate, 3a, top control assembly, 4a, drive control microscope carrier, 5a, reaction box, 6a, 6b, 6c, compression leg,
The first mandrils of 7a-, the second mandrils of 7b-, 7c- thirds mandril, the 4th mandrils of 7d-, 8a- first seal plumb joint, 8b- second is sealed
Plumb joint, 8c- thirds heat-sealing plumb joint, 9a-the PCR reaction warm areas control unit, two PCR of 9b-the reaction warm area controls are single
Member, 9aOnTemperature control module, 9a on-the firstUnder- the first time temperature control module, 9bOnTemperature control module, 9b on-the secondUnder- the second time temperature control mould
The temperature control module of block, PCR reaction warm area control units, 10a, electric rotating machine, 11a, heater element, 12a detection components, 13a, silk
Thick stick driving structure, 14a, guide rail, 15a- fibre-optical probes, the first motors of S1-, S2- gears, S3- connecting shafts, the second motors of S4-,
S5- cams, S6- mounting brackets, the soldering tip of 8a1-U words protrusion, 8a2- welding bases, 8a3- spring resets structure, 8a4- Anchor plate kits.
Specific embodiment
The utility model integration nucleic acid extraction, amplification and detecting system, by sample preparation, nucleic acid extraction, purifying, amplification
And detection and analysis needed for all operationss and function module be integrated in a system, high degree of automation, system bulk are small.Match
Disposable, the closed two-sided disc type reaction box of pre-packaged required reagent is closed, this system can quickly realize that " sample is into as a result
Go out " Automatic nucleic acid Testing analysis.
Disposable, the closed two-sided disc type reaction box of pre-packaged reagent is referring to Publication No. CN106947683A, application
Number be 201710371949 patent of invention.Also reference can be made to Fig. 1 to Fig. 6, the reaction box include matrix 39 and be arranged on matrix 39
On reagent chamber, nucleic acid absorption chamber 16, waste chamber 29, product chamber 35 and foranalysis of nucleic acids unit 21, it is reagent chamber, useless
Sap cavity room 29 and product chamber 35 are communicated by corresponding runner with nucleic acid absorption chamber 16, waste chamber 29 and product chamber 35
By flowing back accordingly, air flue is communicated with each reagent chamber.In the embodiment matrix 39 be it is above-mentioned have it is certain thickness,
Round (shape is not limited to circle, can be other rules or irregular shapes, such as ellipse) disc type apparatus of dimensional double-sided.Base
Body 39 divides for obverse and reverse, and above-mentioned each chamber, foranalysis of nucleic acids unit 21, runner, reflux air flue are located at two-sided disc type respectively
Tow sides of matrix 39 or through with certain thickness matrix stereochemical structure；To reduce the volume of device, and make each chamber
The distribution of room, runner, reflux air flue etc. more rationalizes.
For the convenience of description, 39 front of labeled substrate is A faces, reverse side is B faces.Fig. 1, Fig. 2 are represented respectively in the embodiment
The plan view and stereogram in matrix A faces；Fig. 3, Fig. 4 represent the plan view and stereogram in matrix B faces in the embodiment respectively.Its
In, a shaft card slot 1, multiple reagent chambers and foranalysis of nucleic acids unit 21 are set on matrix A faces；Waste chamber 29 and product
Chamber 35 is set on matrix B faces；Two rotation fixed card slots 2 and nucleic acid absorption chamber 16 run through matrix tow sides.
Wherein, shaft card slot 1 is located at the circle centre position of matrix 39 and with the circle of matrix 39 for whole device to be driven to rotate
Whole round poroid in one centered on the heart, two rotation fixed card slots 2 are oppositely arranged, respectively positioned at the both sides of shaft card slot 1.
During concrete operations, which is fixed on device by shaft card slot 1, rotation fixed card slot 2 drive of Tthe utility model system
On dynamic control microscope carrier, and the unidirectional or crankmotion under the electric rotating machine effect of Tthe utility model system.Produced by rotation
Centrifugation force driving device in liquid flowing, transfer and mixing, to realize whole flows of nucleic acid extraction purification process, and will
Final nucleic acid extraction product is applied to the detection of nucleic acids of follow-up a variety of purposes.In this embodiment, the rotating speed of reaction box rotation
Size is 2000rpm~10000rpm, and adjusting the speed of rotating speed can control liquid that nucleic acid absorption is flowed and flowed through in runner
The speed speed of chamber.In addition, the reaction box can rotate back and forth under the control of mechanical external force, fill the indoor liquid of each chamber
Divide mixing；Or come into full contact with the sorbing material of nucleic acid absorption intracavitary and nucleic acid substances.It should be noted that the shape of shaft card slot
Shape, rotate fixed card slot position and shape be not limited to it is as described herein, as long as rotate fixed card slot installation position realize
It secures the device on relevant device.
Multiple reagent chambers are distributed on the position nearer apart from the center of circle of matrix 39, and each reagent chamber is distributed in matrix and turns
The outside of axis card slot 1, and along 1 circumferential direction of shaft card slot but be not limited to it is circumferentially distributed, for storing nucleic acid extraction and purified reagent.It should
Device generally three to five reagent chambers of setting, the reaction box in the embodiment is provided with four reagent chambers, is first respectively
Reagent chamber 4, the second reagent chamber 7,10 and the 4th reagent chamber 13 of third reagent chamber, it is pre-packaged in the first reagent chamber 4
There is cell pyrolysis liquid and be reserved with the space needed for for adding liquid sample, the first reagent chamber 4 can add in the embodiment
The volume of liquid sample is 100ul~500ul, and liquid sample volume can be according to sample properties, extraction product requirements amount and downstream
Experiment demand and increase and decrease.The adding mouth 3 for adding sample is additionally provided in first reagent chamber 4, is added by the adding mouth 3
In liquid sample to the first reagent chamber 4, in the first reagent chamber 4, the liquid sample that is added by adding mouth 3 with it is pre-packaged
After the abundant mixing of cell pyrolysis liquid, the cell in sample is ruptured and is released under the heat effect of Tthe utility model system heater element
Put cell inclusion (substances such as nucleic acid, albumen, polysaccharide), which flows through nucleic acid absorption chamber 16, the nucleic acid in sample
Substance will be trapped in nucleic acid absorption chamber 16, and liquid waste flows into waste chamber 29 by corresponding runner.
The pre-packaged cleaning solution having needed for washing step in second, third reagent chamber 7,10, cleaning solution is followed by core
Acid absorption chamber 16, for washing in nucleic acid absorption chamber 16 in addition to nucleic acid, the impurity such as protein, polysaccharide are discarded
Liquid flows into waste chamber 29 by corresponding runner.4th reagent chamber 13 is pre-packaged to be had nucleic acid substances from nucleic acid absorption chamber 16
The nucleic acid eluents of middle elution after nucleic acid eluents flow through nucleic acid absorption chamber 16, are captured and are washed in nucleic acid absorption chamber 16
Release is formed nucleic acid extraction purified product, and flow into product chamber by corresponding runner by the nucleic acid substances after washing in solution is eluted
Room 35.Wherein, the quantity of washing step can be according to sample properties, extraction with the reagent chamber quantity of corresponding encapsulation cleaning solution
Time and downstream are tested demand and are increased and decreased.As soon as a reagent chamber for encapsulation cleaning solution, such reagent chamber can be such as set
It is three；The reagent chamber of two encapsulation cleaning solutions can also be set, such reagent chamber is exactly four, and so on.
Be both provided with reagent flow road junction and reflux gas port corresponding with reagent flow road junction in each reagent chamber, i.e., first
The first reagent flow road junction 5 and the first reflux gas port 6 are provided in reagent chamber 4, the second examination is provided in the second reagent chamber 7
Agent runner mouth 8 and second flows back gas port 9, is provided with third reagent flow road junction 11 and third backflow gas in third reagent chamber 10
Road junction 12 is provided with the 4th reagent flow road junction 14 and the 4th reflux gas port 15 in the 4th reagent chamber 13.Each reagent chamber
It is connected by respective reagent flow road junction with nucleic acid absorption chamber 16, and passes through respective reflux gas port and waste chamber 29
It is connected with product chamber 35.In the embodiment, the reagent flow road junction in each reagent chamber is close to the outside of reagent chamber
Wall is set, and to be formed by the be recessed outward semicircular arc-shaped side groove and being recessed downwards from chamber bottom surface of formation of the lateral wall from chamber
Circular groove composition.Reflux gas port in each reagent chamber is close to the madial wall setting of reagent chamber, and is certainly
Chamber bottom surface is recessed downwards the circular groove of formation.Certainly, the shape of the reagent flow road junction in reagent chamber and reflux gas port
It is not limited to as described herein.
Nucleic acid absorption chamber 16 is through 39 tow sides of matrix and on the outside of reagent chamber, for capturing in liquid sample
Nucleic acid substances.Comprising above-mentioned for capturing the sorbing material of nucleic acid substances in nucleic acid absorption chamber 16, which includes
But it is not limited to the sorbing materials such as glass fibre, Silicon moulds or glass microballoon.Sorbing material (can such as exist in corresponding reagent and external force
Heating and Nucleic Acid Elution reagent) cooperation under, by the nucleic acid substances captured release in buffer solution, to realize that nucleic acid extraction is pure
Change purpose.
Nucleic acid absorption chamber 16 is connected by reagent runner 24 with each reagent chamber, reagent runner 24 and each chamber
Reagent flow road junction be connected, i.e., first, second, third, fourth reagent flow road junction 5,8,11,14 is connected with reagent runner 24
It is logical, so as to fulfill being connected for each reagent chamber and nucleic acid absorption chamber 16.When it is implemented, each reagent chamber can lead to
A common reagent runner 24 or being connected by respective reagent runner 24 and nucleic acid absorption chamber 16 are spent, that is,
Say that the indoor lysate of reagent chamber, cleaning solution and eluent reagent can be by public reagent runners 24 followed by nucleic acid absorption chamber
Room 16.In the embodiment, reagent runner 24 is distributed in the B faces of matrix, and is common.
The outside of nucleic acid absorption chamber 16 is additionally provided with waste liquor stream road junction 17 and product stream road junction 18, in the embodiment, core
Acid absorption chamber 16, waste liquor stream road junction 17, product stream road junction 18 are located in same groove.Waste liquor stream road junction 17 connects nucleic acid absorption
Chamber 16 and waste chamber 29, the interior liquid waste generated of each reagent chamber pass through the waste liquid runner under the influence of centrifugal force
Mouth 17 flows into waste chambers 29；Product stream road junction 18 connects nucleic acid absorption chamber 16 and product chamber 35, nucleic acid absorption chamber 16
It is inside released the nucleic acid product to be formed and product chamber 35 is flowed by the product stream road junction 18 under the influence of centrifugal force.
Waste chamber 29 and product chamber 35 are distributed in the outside of nucleic acid absorption chamber 16 on the radial direction of matrix
And be connected by corresponding runner with nucleic acid absorption chamber 16, it is respectively used to liquid waste after collecting reaction and is carried with nucleic acid is collected
Take purified product.Wherein, waste chamber 29 is interconnected by waste liquid runner (not shown) and nucleic acid absorption chamber 16, and nucleic acid is inhaled
The waste liquid flowed out in attached chamber 16 flows into waste chamber 29 by waste liquid runner；Specifically, waste chamber 29 and waste liquid runner phase
Connection, waste liquid runner are connected with waste liquor stream road junction 17, and waste liquor stream road junction 17 is connected with nucleic acid absorption chamber 16, so as to fulfill
The connection of waste chamber 29 and nucleic acid absorption chamber 16.By product runner, (figure is not with nucleic acid absorption chamber 16 for product chamber 35
Show) it is interconnected, the nucleic acid extraction purified product flowed out in nucleic acid absorption chamber 16 flows into product chamber 35 by product runner；Tool
For body, product chamber 35 is connected with product runner, and product runner is connected with product stream road junction 18, product stream road junction 18 with
Nucleic acid absorption chamber 16 is connected, it is achieved thereby that the connection of product chamber 35 and nucleic acid absorption chamber 16.
In addition, in order to control and ensure that liquid flows in locking device, waste chamber 29 and product chamber 35 are by right
The reflux air flue answered is connected with each reagent chamber.The embodiment, waste chamber are flowed back air flue 30 and the by the first reagent
One reagent chamber 4, the second reagent chamber 7 are connected with third reagent chamber 10, specifically, the first reagent reflux air flue 30 with
Reflux gas port 6,9,12 in first, second, and third reagent chamber 4,7,10 is connected, so as to fulfill waste chamber 29
With being connected for first, second, and third reagent chamber 4,7,10；Product chamber 35 is flowed back air flue 40 and the by the second reagent
Four reagent chambers 13 are connected, specifically, the 4th backflow gas in the second reagent reflux 40 and the 4th reagent chamber 13 of air flue
Road junction 15 is connected, so as to fulfill being connected for product chamber 35 and the 4th reagent chamber 13.It is additionally provided on product chamber 35
Runner mouth 37 is analyzed, is provided with and analyzes back on the second reagent reflux air flue 40 that product chamber 35 is connected with the 4th reagent chamber 13
Gas road junction 38.
Preferably, in order to which the indoor reagent of each reagent chamber is controlled to flow through nucleic acid absorption chamber 16 in a certain order,
In the reagent runner 24 that each reagent chamber is connected with nucleic acid absorption chamber 16, corresponding each reagent chamber is provided with accordingly
Runner valve, respectively the first reagent runner valve 25, the second reagent runner valve 26, third reagent runner valve 27 and the 4th reagent flow
Road valve 28, respective valves are opened successively according to nucleic acid extraction purifying flow, to ensure that different reagents flow successively according to certain sequence
Through nucleic acid absorption chamber 16, i.e. the first reagent runner valve 25, the second reagent runner valve 26, third reagent runner valve 27 and the 4th try
Agent runner valve 28 is opened successively according to sequencing, so that the corresponding indoor liquid of reagent chamber is followed by nucleic acid absorption chamber
16。
Preferably, it is also equipped in the reflux air flue that each reagent chamber is connected with waste chamber 29, product chamber 35
With the indoor corresponding return valve of runner valve of reagent chamber.In the embodiment, waste chamber 29 and first, second, and third
In the first reagent reflux air flue 30 that reagent chamber 4,7,10 is connected, corresponding first, second, and third reagent chamber 4,7,10
It is provided with corresponding return valve, the respectively first reflux airway valve 31, second reflux airway valve 32 and third reflux airway valve
33；In the second reagent reflux air flue 40 that product chamber 35 is connected with each reagent chamber, corresponding 4th reagent chamber 13 is set
Corresponding return valve is equipped with, for the 4th reflux airway valve 34.
Preferably, it in the product runner that nucleic acid absorption chamber 16 is connected with product chamber 35, is provided with and the 4th backflow gas
34 corresponding product runner valve 36 of road valve；It is also equipped in the waste liquid runner that waste chamber 29 is connected with nucleic acid absorption chamber 16
Waste liquid runner valve (not shown), waste liquid runner valve can be in the effects of Tthe utility model system switching device (such as mandril punctures head)
It is opened under lower outer force-fitting, the cell pyrolysis liquid and cleaning solution discarded after reaction is made to flow into waste chamber 29 by waste liquid runner；It should
Valve can be again switched off under outer force-fitting under the action of Tthe utility model system switching device (such as heat-sealing plumb joint), to ensure
Waste liquid in waste chamber 29 no longer flows out, while there is no other liquid inflow waste chambers 29.
Foranalysis of nucleic acids unit 21 is radially disposed in the outside of product chamber 35 in matrix, and is connected with product chamber 35, product
Nucleic acid extraction purified product in chamber 35 flows into foranalysis of nucleic acids unit 21 by corresponding runner, analyzes and examines for nucleic acid product
It surveys.Specifically, foranalysis of nucleic acids unit 21 is connected with the analysis runner mouth 37 on product chamber 35, so as to connect foranalysis of nucleic acids list
Member 21 and product chamber 35.Foranalysis of nucleic acids unit 21 is also connected by analysis reflux air flue 41 with product chamber 35, specifically,
Analysis reflux air flue 41 is connected with the analysis reflux gas port 38 on the second reagent reflux air flue 40.Foranalysis of nucleic acids unit 21 exists
The external hot circulation and optics of Tthe utility model system temperature control component and detection components (nucleic acid rapid amplifying and detecting system) are examined
It surveys under the cooperation of module, can realize the detection functions such as nucleic acid PCR amplification, real-time fluorescence detection.
It should be noted that liquid is at runner (reagent runner 24, waste liquid runner, product runner, analysis runner 22 or 22')
In interior flow process, the corresponding reflux air flue of this runner (the first reagent reflux air flue 30, the second reagent reflux air flue
40th, analysis reflux air flue 41) in respective valves will be all turned on, to ensure the air pressure balance in locking device, enable liquid
It is enough to be smoothly transferred to another chamber from a chamber.Before reagent runner being flowed into such as the mixing liquid in the first reagent chamber 4,
The first reagent runner valve 25 and the first reflux airway valve 32 are opened simultaneously, and the second~the 4th reagent chamber is similar；For another example product chamber
Nucleic acid product in room 35 enters before foranalysis of nucleic acids unit 21, need to open analysis runner valve 19, analysis reflux airway valve simultaneously
20.Valve cooperation unlatching/closing in runner inner flow passage valve and reflux air flue, can control and ensure liquid in locking device
Interior flowing.In addition, before using the utility model device, set valve will close in all runners, reflux air flue, pass through
After 3 adding liquid sample of adding mouth, pass through heat-sealing under the action of Tthe utility model system switching device (such as heat-sealing plumb joint)
Adding mouth 3 is sealed.
Preferably, runner, valve set in reflux air flue can be puncture valve, and puncture valve is opened in Tthe utility model system
It will be punctured under the action of the external pressing structure (not shown) of pass device (such as puncturing head), the runner, reflux air flue will be switched on.
Outside the switching device of Tthe utility model system pressing structure can with one runner of a secondary control, the air flue that flows back conducting and close
Close, the multiple runners of a secondary control that can also combine, reflux air flue while be connected and be closed.In the embodiment party of certain replacements
In formula, set valve can be heat-sealing valve in the runner of the utility model device, the air flue that flows back, and heat-sealing valve is in the utility model
It will be opened under the action of the heating of system switching device exterior, heat again, the valve will be again switched off.The utility model is implemented
What the valve set in example 1 was selected is puncture valve.
Preferably, nucleic acid extraction purifying dimensional double-sided disc type apparatus is made of at least one plastics, resin material, including poly-
Ethylene, low density polyethylene (LDPE), polypropylene, polyvinyl chloride etc..Chamber that reaction box is provided (each reagent chamber, waste chamber,
Product chamber) it can be formed in rigid plastics material by least one layer of plastic film material plastic packaging, it can also be by least two layers
Film-form plastic material seals to be formed, and the construction of chamber may include various shapes, such as fan-shaped, round, oval, water-drop-shaped or not
Regular shape.
Fig. 5, Fig. 6 represent reaction box A faces and B faces sealer welding figure.Chamber, runner included by reaction box, reflux air flue,
Foranalysis of nucleic acids unit and card slot, be on certain thickness disc type rigid plastics, two-sided engraving, three-dimension process, and
The welding of rigid plastics upper sealing film is covered in by plastic film material to complete, the gray area in Fig. 5, Fig. 6 represents the utility model
Device needs the region welded during manufacturing.
Specifically, in some embodiments, runner (reagent runner, product runner, the waste liquor stream that reaction box is provided
Road) it can be formed in rigid plastics material by least one layer of plastic film material plastic packaging, it can also be membranaceous by least two layers of thin
Plastic material seals to be formed, and the construction of runner may include various shapes, such as linear type, arc-shaped or irregular shape.
As shown in Fig. 7, Fig. 8, Fig. 9 and Figure 10, a kind of integrated nucleic acid extraction provided by the utility model, amplification and detection
System includes drive control the microscope carrier 4a, Yi Jiyi for loading, rotating and acted on from bottom two-sided disc type reaction box application
A top control assembly 3a acted on from top same reaction box application.Drive control microscope carrier 4a and top control assembly 3a by
Connection component is connected.
In some embodiments, the connection component includes level to move horizontally component, the component that moves horizontally
The leading screw driving structure 13a of setting and guide rail 14a, the drive control microscope carrier 4a is in the driving lower edge of leading screw driving structure 13a
Guide rail 14a is translated between the underface and side of top control assembly 3a.
In some embodiments, the connection component is vertical motion assemblies, and the vertical motion assemblies include vertical
The leading screw driving structure and guide rail of setting, the top control assembly are close or remote along guide rail under the driving of leading screw driving structure
From drive control microscope carrier.
In some embodiments, the connection component is overturning component, and the overturning component includes controlling positioned at top
Articulated structure and driving motor between component and drive control microscope carrier；The top control assembly is in the driving of driving motor
Under around articulated structure overturn.
A kind of integrated nucleic acid extraction provided by the utility model, amplification and detecting system, drive control microscope carrier 4a with
The performer for acting perpendicularly to two-sided disc type reaction box is installed on the control assembly 3a of top, packet electric machine assembly, hold down assembly,
Temperature control component, switching device, detection components and drive component etc..
The electric machine assembly includes the electric rotating machine 10a being located on drive control microscope carrier 4a, the rotation of the electric rotating machine 10a
Shaft is corresponding with the rotation fixed card slot 2 of two-sided disc type reaction box circle centre position, and reaction box can be fixed on to the screens of rotary shaft
On.
In some embodiments, the cross section of the rotary shaft can be round or irregular shape, have
Guidance quality, this will make reaction box can only in a certain direction, angle lateral load is in the screens of rotary shaft.
In some embodiments, the rotary shaft is equipped with screw thread, for loading, fixing reaction box.Furthermore, it is possible to
Step up fixed reaction box so that it can follow centrifugation motor high speed rotation with nut in rotary shaft.
Electric rotating machine 10a can drive loaded reaction box 5a rotational positionings, unidirectional or reciprocating rotary, high speed centrifugation with
The flowing of driving liquid makes liquid blending in cavity, and this system can regulate and control time, angle and the rotating speed of rotation.
In some embodiments, this system can control electric rotating machine 10a single direction rotations, drive loaded reaction box
5a any rotations make cavity, runner, valve of reaction box 5a etc. be located in drive control microscope carrier 4a or top control assembly
3a carries out it corresponding position needed for peripheral operation.
In some embodiments, this system can control electric rotating machine 10a reciprocating rotaries, in the cavity for making reaction box 5a
Liquid is quick, abundant mixing；The PCR chambers that can also make reaction box 5a are quick between different PCR warm area control units
It shifts back and forth, to realize nucleic acid rapid amplifying.
In some embodiments, this system can control electric rotating machine 10a high speed rotations, the centrifugation that high speed rotation generates
Power can drive the flowing of liquid in reaction box 5a.Under suitable rotating speed, no matter the runner between cavity and cavity is distributed
In the front of reaction box 5a or reverse side, the centrifugal force that the unidirectional high speed rotations of reaction box 5a generate can drive liquid anti-from distance
The cavity for answering box 5a centers near flows to the cavity remote apart from reaction box 5a centers.
The rotating speed of electric rotating machine 10a can be with dynamic regulation, and rotating speed size is 2000rpm~10000rpm.Adjust rotating speed
Speed can control liquid in reaction box 5a that the speed speed of some chamber is flowed and flowed through in runner.Carrying out column embrane method
Nucleic acid absorption, washing and the efficiency of elution are adjusted when nucleic acid extraction purifies, obtains best nucleic acid yield and purity.
It is described to hold down assembly in the control assembly of top, for the compression of reaction box 5a；In some embodiments, institute
It states to hold down assembly and includes the compression leg of at least three circumferentially equidistantly distributeds, it is anti-that system can control compression leg to compress downwards simultaneously
Box 5a is answered, it is made to be fixed on position location, other performers of drive control microscope carrier 4a and top control assembly 3a are to reaction
Box 5a applications act on carrying out corresponding operating.
In some embodiments, when each performer by flow complete operation after, system can control compression leg simultaneously to
Upper withdrawal, release reaction box 5a continue other operating processes.
The switching device be used to open or capping box in various switches.In some embodiments, it is described to open
It can be used to open or the mandril of capping box inner valve or the thorn for puncturing Thin-membrance valves in reaction box to close device
The heat-sealing plumb joint of adding mouth, Thin-membrance valves or runner in broken needle or capping box.
In some embodiments, the mandril, lancet surface can be the stereochemical structure of planar structure or protrusion,
Its cross section can be round, rectangular etc., or irregular shape.This system can control single or disposable same time control
It makes multiple mandrils or punctures head and carry out runner conducting operation, be connected while reaction box one or more valve, runner with realizing.
In some embodiments, heat-sealing welding head surface can be the stereochemical structure of planar structure or protrusion, transversal
Face can be round, rectangular, fan-shaped, shape of a hoof etc., or irregular shape；There are one one heat-sealing plumb joint can be gathered around
Or multiple welding positions, on reaction box one or more points, one or more region is subjected to.This system can be with
Single heat-sealing plumb joint or disposable while control multiple heat-sealing plumb joints is controlled, for the correspondence position of high-temperature soldering reaction box
It puts, meanwhile, this system can regulate and control weld interval and the temperature of welding module.
The temperature control component includes two or more sets PCR warm area control units, and every group of PCR warm areas control unit includes two
The opposite temperature control module for being mounted on top control assembly and drive control microscope carrier, the control temperature of two temperature control modules is identical, shape
Warm area in into reaction box needed for PCR reactions, the control temperature of different PCR warm areas control units are different.
In some embodiments, upper and lower two temperature control modules can clamp two-sided disc type reaction box by system control
PCR reaction cavity regions, reverse transcription or the temperature needed for PCR amplification are provided, can be carried out at the same time together in reaction box tow sides
The region control of one temperature.
In some embodiments, this system is provided with two or more sets PCR warm area control units, the amplification of reaction box 5a
Chamber quickly can back and forth shift in different warm area control units under the drive of electric rotating machine 11a, meet PCR amplification institute
The temperature requirements needed realize the rapid amplifying reaction of nucleic acid.
The driving component at least there are two, be respectively arranged on the control assembly 3a and drive control microscope carrier 4a of top, it is real
It now holds down assembly, the pushing of temperature control component, heater element, switching device, detection components or upper lift.
In some embodiments, the driving component includes translation structure and cam structure；The translation structure is used for
Cam structure is moved to the driving end of the performer of implementation；The cam structure is used to drive corresponding performer
Pushing or upper lift.
In some embodiments, the driving component can be used for driving two temperature up and down of every group of warm area control unit
Control the PCR reaction cavity regions that module clamps reaction box；
In some embodiments, it is provided on the performer of the drive control microscope carrier 4a and top control assembly 3a
Spring structure, the spring structure can make performer cam effect under on lift or push, ensure performer with reacting
Box comes into full contact with (such as abundant top pressure is punctured, welded, heating, magnetic), while avoid the bending or breakage of performer.
A kind of integrated nucleic acid extraction provided by the utility model, amplification and detecting system, drive control microscope carrier and top
The performers such as heater element and/or magnetic device are additionally provided in portion's control assembly, are used to implement column embrane method or paramagnetic particle method core
Acid extraction flow.
The heater element be used for reaction box nucleic acid extraction purification during lysate, eluent heating.It is described
Temperature, time and the temperature rate that heater element can be opened under system control, close, adjust heating.
In some embodiments, the surface of heater element can be the stereochemical structure of planar structure or protrusion, transversal
Face can be round, rectangular, fan-shaped, shape of a hoof etc., or irregular shape；Heater element can be in the work of drive component
The surface of reaction box corresponding chambers is tightly attached under, it is quick provide crack during nucleic acid extraction purifying and etc. needed for heating
Temperature.
The magnetic assembly is used for the magnetic bead adsorption operations in paramagnetic particle method extraction process.The magnetic device can be
The lower intensity for opening, closing, adjusting magnetic of system control and time.In some embodiments, magnetic device can be with drive component
Under the action of be tightly attached to the surfaces of reaction box corresponding chambers, quick absorption, transfer or the release for realizing magnetic bead in reaction box.
Below in conjunction with the attached drawing of the utility model one embodiment, the technical solution of the utility model embodiment is carried out
Description.
As shown in Fig. 7, Fig. 8, Fig. 9 and Figure 10, by left plate 2a and right plate, (right plate is not shown top control assembly
Show) it is fixed on instrument base 1a, drive control microscope carrier 4a is connected to by guide rail 14a on instrument left plate 2a and right plate.
Be provided on the control assembly 3a of top the first compression leg 6a, the second compression leg 6b, the second compression leg 6c, the first mandril 7a, the second mandril 7b,
The heat-sealing plumb joints of second mandril 7c and first 8a, two PCR react temperature control module 9a on the first of warm area control unitOnAnd second
Upper temperature control module 9bOn, wherein temperature control module 9b on secondOnOn the first fibre-optical probe 12a, the second fibre-optical probe 12b, are installed
Three fibre-optical probe 12c, the 4th fibre-optical probe 12d and the 5th fibre-optical probe 12e；The 4th mandril is provided on drive control microscope carrier 4a
7d, the second heat-sealing plumb joint 8b, third heat-sealing plumb joint 8c, an electric rotating machine 10a, a heater element 11a, two PCR
React first time temperature control module 9a of warm area control unitUnderAnd second time temperature control module 9bUnder。
In this implementation column, after the adding mouth addition at the top of reaction box, the adding mouth of reaction box will be sealed liquid sample,
Form the system of closing.Each row device of the utility model carries out column embrane method nucleic acid by under the control of system program later
Extraction purification flow, the nucleic acid extraction product obtained will flow under the driving of system centrifugal force and be distributed in reaction box outmost turns
Multiple pre-packaged freeze-dried reagents PCR reaction chambers, it is final to realize nucleic acid rapid PCR amplification and under the control of system program
And detection.
Before bringing into operation, Tthe utility model system control drive control microscope carrier moves to loading position, by added mistake
The two-sided disc type reaction tray of sample is loaded on drive control microscope carrier, as shown in Figure 7.Subsequent drive control microscope carrier moves to operation
Position, electric rotating machine 10a drive two-sided disc type reaction box 5a rotations, make the first heat-sealing upper with top control assembly 3a of its adding mouth
Plumb joint 8a is corresponding, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c to compress reaction box 5a downwards simultaneously,
It is made to be fixed on position location, the first heat-sealing plumb joint of system control 8a, which is pushed, seals adding mouth at the top of reaction box, system
The first compression leg 6a, the second compression leg 6b, third compression leg 6c is controlled to withdraw release reaction box upwards simultaneously.
After the sealing of reaction box adding mouth, system will proceed by nucleic acid extraction flow, and the sample added in reaction box will
It is cleaved, the nucleic acid in sample will be captured, washing, elute.In the process, the unlatching of reaction box runner is by electric rotating machine
10a drives two-sided disc type reaction box 5a rotation, make the corresponding runner of reaction box in the control assembly of top the first mandril 7a, the
Two mandril 7b or third mandril 7c are corresponding, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c to be pressed downward simultaneously
Tight reaction box 5a, fixes it；System controls the first mandril 7a, the second mandril 7b or third mandril 7c to push and opens reaction box
Valve on corresponding runner；After corresponding runner is opened, system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c simultaneously
Release reaction box is withdrawn upwards.Liquid flowing in reaction box runner is to drive two-sided disc type reaction box 5a by electric rotating machine 10a
Rotate what high speed centrifugation rotation driving was completed.Liquid is after runner is transferred to corresponding chambers in reaction box, each indoor liquid of chamber
Body mixing is two-sided disc type reaction box 5a reciprocating rotaries to be driven to complete by electric rotating machine 10a.Crack during nucleic acid extraction and
Heating temperature needed for two step process is eluted, two-sided disc type reaction box 5a is driven to rotate by electric rotating machine 10a, makes its phase
Answer chamber corresponding with the heater element 11a on drive control microscope carrier 4a, system controls the first compression leg 6a, the second compression leg 6b, the
Three compression leg 6c compress downwards reaction box 5a simultaneously, fix it；Reaction box is close in system control mandril heater element 11a pushings
Corresponding chambers are heated；After the completion of heating, system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c upward simultaneously
Withdraw release reaction box.The waste liquid generated during nucleic acid extraction will enter waste liquid chamber by waste liquid runner by centrifugation.
After nucleic acid extraction process, electric rotating machine 10a drives two-sided disc type reaction box 5a rotation, make its waste liquid runner with
The upper second heat-sealing plumb joint 8b of drive control microscope carrier 4a are corresponding, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg
6c compresses downwards reaction box 5a simultaneously, it is made to be fixed on position location, and lift will from reaction box bottom on the second heat-sealing plumb joint 8b
Waste liquid runner is closed, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c to withdraw release reaction box upwards simultaneously.
Then, electric rotating machine 10a drives two-sided disc type reaction box 5a rotation, makes the of PCR reaction chambers runner and drive control microscope carrier 4a
Four mandril 7d are corresponding, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c to compress reaction box 5a downwards simultaneously,
It is made to be fixed on position location, system controls the valve on the PCR reaction chamber runners of lift opening reaction box on the 4th mandril 7d, opens
PCR reaction chamber runners are opened, system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c to withdraw release reaction upwards simultaneously
Box.Electric rotating machine 10a drives two-sided disc type reaction box 5a high speed centrifugations rotation, and driving nucleic acid extraction product is through PCR reaction chamber streams
Road flows into PCR reaction chambers, and electric rotating machine 10a drives two-sided disc type reaction box 5a rotations, makes PCR reaction chambers runner and drive control
Third heat-sealing plumb joint 8c on microscope carrier 4a is corresponding, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c simultaneously
Reaction box 5a is compressed downwards, it is made to be fixed on position location, lifts on third heat-sealing plumb joint 8c and is reacted from reaction box bottom by PCR
Chamber runner is closed, and system controls the first compression leg 6a, the second compression leg 6b, third compression leg 6c to withdraw release reaction box upwards simultaneously.
After the closing of PCR reaction chambers runner, system will proceed by nucleic acid rapid PCR amplification and testing process, electric rotating machine
10a drives the mixing of liquid and the powdered reagent of encapsulation that two-sided disc type reaction box 5a reciprocating rotaries are completed in PCR reaction chambers；It is mixed
After even, electric rotating machine 10a drives two-sided disc type reaction box 5a to react warm area control unit 9a and the 2nd PCR reactions in the first PCR
It is quick mobile back and forth between warm area control unit 9b, to carry out PCR amplification.As shown in Figure 10 a, when two-sided disc type reaction box 5a's
When PCR reaction chambers are moved to the first PCR reaction warm area control unit 9a under the drive of electric rotating machine 10a, the first PCR reaction temperature
Temperature control module 9a on the first of area control unit 9aOnAnd first time temperature control module 9aUnderPCR reaction chambers can be clamped and carry out temperature
Degree control；As shown in fig. lob, when the PCR reaction chambers of two-sided disc type reaction box 5a are moved under the drive of electric rotating machine 10a
When two PCR react warm area control unit 9b, the 2nd PCR reacts temperature control module 9b on the second of warm area control unit 9bOnAnd second
Lower temperature control module 9bUnderPCR reaction chambers can be clamped to trip temperature control of going forward side by side, meanwhile, temperature control module 9b on secondOnUpper installation
Five fibre-optical probe 15a, which can react PCR, carries out multichannel fluoroscopic examination, realizes rapid amplifying and the detection of nucleic acid.
In above-mentioned all processes, the rotating speed of electric rotating machine 10a can carry out dynamic regulation according to experiment demand.Figure 11 is represented
The utility model electric rotating machine 10a speed curves dynamic regulation examples, wherein f1, f2 are different frequency, and a1, a2 are different width
Value.The drive control microscope carrier electric rotating machine is equipped with coding disk, can to the absolute position of two-sided disc type reaction box and rotating speed into
Row negative feedback control.For the rotating speed of the reaction box up to 2000~10000 revs/min, the rate curve of rotating speed control can be with
It is triangular wave, sawtooth wave, sine wave or parabola etc., the frequency and amplitude of speed curves can be with dynamic regulations, and then realize
In reaction box in different cavity body different volumes liquid quick mixing.
In above-mentioned all processes, each actuator at the top of Tthe utility model system in control assembly and drive control microscope carrier
Part needs to lift or push under the action of drive component.Figure 12 a, Figure 12 b represent control assembly at the top of Tthe utility model system
Upper one specific embodiment of drive component, drive component are included by the connecting shaft S3 rotational structures connected and cam structure, rotation
Structure includes the first motor S1, gear S2 and connecting shaft S3；Cam structure includes the second motor S4, cam S5 and mounting bracket S6.
Mounting bracket S6 is used for the installation of cam S5 and the second motor S4, and mounting bracket S6 is fixedly connected with the other end of connecting shaft S3, and first
Motor S1 is rotated horizontally with moving gear S2 and the connecting shaft S3 connected to above the performer that implementation is needed to act on, the second electricity
Machine S4 drives connected cam S5 axial-rotations counterclockwise, and the particular architectures of cam S5 hold corresponding in rotary course
Row device pushes, to realize its corresponding concrete operations.
Figure 13 a, 13b, 13c seal some specific implementation row of plumb joint for Tthe utility model system.As depicted in fig. 13 a,
The embodiment seals plumb joint for a U-shaped in the control assembly of top, and U-shaped heat-sealing plumb joint includes end face and sets U
The soldering tip 8a1 of word protrusion, welding base 8a2, spring reset structure 8a3 and Anchor plate kit 8a4, the soldering tip 8a1 are answered by spring
Bit architecture 8a3 is installed on 8a2 on welding base, and the Anchor plate kit 8a4 is fixedly connected with soldering tip 8a1 for by the drive of drive component
Power passes to soldering tip 8a1, which can push under the action of cam, is close to the sample-adding mouth region of reaction box consumptive material
Domain is welded, and adding mouth is sealed.As illustrated in fig. 13b, which is a strip on drive control microscope carrier
Shape seals plumb joint, and the end face of strip heat-sealing plumb joint is provided with strip projected parts, and lift can be controlled by system and will be reacted
Corresponding runner closing on box.As shown in figure 13 c, which is a heat-sealing welding on drive control microscope carrier
Head, there are five welding sites for heat-sealing plumb joint tool, can be controlled and lifted by system, disposable to weld on reaction box five simultaneously
Corresponding site.
Claims (10)
1. fully automatic integral nucleic acid extraction, amplification and detecting system, it is characterised in that：
Drive control microscope carrier (4a) including top control assembly (3a), below top control assembly (3a) and for adjusting
The connection component of relative position between top control assembly (3a) and drive control microscope carrier (4a)；
Multiple performers, the performer packet are provided on the top control assembly (3a) and drive control microscope carrier (4a)
It includes electric machine assembly, hold down assembly, temperature control component, switching device, detection components and drive component；Wherein：
The electric machine assembly includes the electric rotating machine (10a) being located on drive control microscope carrier (4a), and the electric rotating machine (10a) is used
In the rotation of reaction box (5a)；
It is described to hold down assembly in top control assembly (3a), for the compression of reaction box (5a)；
The temperature control component includes two or more sets PCR warm area control units, and every group of PCR warm areas control unit includes two relatively
Temperature control module in top control assembly (3a) and drive control microscope carrier (4a), the control temperature phase of two temperature control modules
Together, the warm area needed for PCR reactions in reaction box is formed, the control temperature of different PCR warm areas control units is different；
The switching device be used to open or capping box in various switches；
The detection that the detection components are reacted for PCR in reaction box；
The driving component at least there are two, be respectively arranged in top control assembly (3a) and drive control microscope carrier (4a) on, reality
It now holds down assembly, pushing or the upper lift of temperature control component, heater element, switching device and detection components.
2. fully automatic integral nucleic acid extraction according to claim 1, amplification and detecting system, it is characterised in that：
The connection component includes horizontally disposed leading screw driving structure to move horizontally component, the component that moves horizontally
(13a) and guide rail (14a), the drive control microscope carrier (4a) exist under the driving of leading screw driving structure (13a) along guide rail (14a)
It is translated between the underface and side of top control assembly (3a).
3. fully automatic integral nucleic acid extraction according to claim 1, amplification and detecting system, it is characterised in that：
The connection component is vertical motion assemblies, and the vertical motion assemblies include vertically disposed leading screw driving structure and lead
Rail, the top control assembly (3a) is under the driving of leading screw driving structure along guide rail closer or far from drive control microscope carrier
(4a)。
4. fully automatic integral nucleic acid extraction according to claim 1, amplification and detecting system, it is characterised in that：
The connection component is overturning component, and the overturning component includes being located at top control assembly (3a) and drive control microscope carrier
Articulated structure and driving motor between (4a)；The top control assembly (3a) is under the driving of driving motor around hinged knot
Structure is overturn.
5. fully automatic integral nucleic acid extraction, amplification and detecting system according to claims 1 or 2 or 3 or 4, feature
It is：
Heater element (11a) and/or magnet suction device are additionally provided on the top control assembly (3a) and drive control microscope carrier (4a)
Part, wherein：
The heater element (11a) for lysate, eluent during reaction box nucleic acid extraction purification heating；
The magnetic assembly is used for the magnetic bead adsorption operations in paramagnetic particle method extraction process.
6. fully automatic integral nucleic acid extraction, amplification and detecting system according to claims 1 or 2 or 3 or 4, feature
It is：
The electric machine assembly further includes coding disk, for the absolute position of reaction box and the negative feedback control of rotating speed；
Described to hold down assembly including at least three circumferential compression legs, the driving structure driving compression leg moves up and down；
The detection components include multiple fibre-optical probes, and it is glimmering that the fibre-optical probe carries out multichannel to PCR reactions multiple in reaction box
Light excitation-detection；
The driving component includes rotational structure and cam structure；The rotational structure is used to cam structure turning to implementation work
The driving end of the performer of work；The cam structure is used to drive corresponding performer pushing or upper lift；
The switching device includes mandril, lancet and/or heat-sealing plumb joint；The mandril be used to open or capping box in
Valve, for the lancet for puncturing sealing film, the heat-sealing plumb joint is used to close the sealing of adding mouth or runner
Film.
7. fully automatic integral nucleic acid extraction according to claim 6, amplification and detecting system, it is characterised in that：
The detection components are arranged on the temperature control module of a PCR warm area control unit of top drive.
8. fully automatic integral nucleic acid extraction according to claim 6, amplification and detecting system, it is characterised in that：
The rotary shaft of the electric rotating machine (10a) is corresponding with the fixed card slot of reaction box circle centre position, can be fixed on reaction box
In the screens of rotary shaft；
The electric rotating machine rotating speed controlling curve is triangular wave, sawtooth wave, sine wave or parabola.
9. fully automatic integral nucleic acid extraction according to claim 6, amplification and detecting system, it is characterised in that：
Rotational structure in the driving component includes the first motor (S1), gear (S2) and connecting shaft (S3), first electricity
Machine (S1) is horizontal positioned, and the thread being meshed with gear (S2), the gear are provided on the output shaft of the first motor (S1)
(S2) mounted on one end of connecting shaft (S3), the connecting shaft (S3) is vertical with the output shaft of the first motor (S1), the gear
(S2) output shaft with the first motor (S1) engages, and first motor (S1) is for band moving gear (S2) and connecting shaft (S3) rotation
Turn；
The cam structure includes mounting bracket (S6), cam (S5) and the second motor (S4), and the mounting bracket (S6) is for cam
(S5) and the installation of the second motor (S4), the mounting bracket (S6) are fixedly connected with the other end of connecting shaft (S3), the cam
(S5) horizontally disposed with the second motor (S4), second motor (S4) rotates for band moving cam.
10. fully automatic integral nucleic acid extraction according to claim 6, amplification and detecting system, it is characterised in that：
The heat-sealing plumb joint includes U-shaped heat-sealing plumb joint, strip heat-sealing plumb joint and more solder joints heat-sealing plumb joint；
The U-shaped heat-sealing plumb joint includes sealing for adding mouth, and the U-shaped heat-sealing plumb joint includes end face setting U-shaped protrusion
Soldering tip (8a1), welding base (8a2), spring reset structure (8a3) and Anchor plate kit (8a4), the soldering tip (8a1) passes through bullet
Spring resetting structure (8a3) is installed on welding base (8a2), and the Anchor plate kit (8a4) is fixedly connected to incite somebody to action with soldering tip (8a1)
The driving force of drive component passes to soldering tip (8a1)；
Strip heat-sealing plumb joint is used for the closing of runner, and it is convex that the end face of the strip heat-sealing plumb joint is provided with strip
It rises；
More solder joint heat-sealing plumb joints are used to weld multiple corresponding sites on reaction box simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483493.4U CN207537439U (en) | 2017-11-08 | 2017-11-08 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721483493.4U CN207537439U (en) | 2017-11-08 | 2017-11-08 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207537439U true CN207537439U (en) | 2018-06-26 |
Family
ID=62612673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721483493.4U Withdrawn - After Issue CN207537439U (en) | 2017-11-08 | 2017-11-08 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
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|---|---|
| CN (1) | CN207537439U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603859A (en) * | 2017-11-08 | 2018-01-19 | 西安天隆科技有限公司 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
| CN111187718A (en) * | 2020-02-21 | 2020-05-22 | 厦门大学 | Nucleic acid extraction device and nucleic acid detection system |
| CN111607509A (en) * | 2019-02-22 | 2020-09-01 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN112439468A (en) * | 2019-08-30 | 2021-03-05 | 天津大学 | Rotary thermal circulation type multi-scale liquid drop digital polymerase chain reaction instrument system |
| CN113564044A (en) * | 2021-08-04 | 2021-10-29 | 圣湘生物科技股份有限公司 | Nucleic acid detection device and nucleic acid detection method |
-
2017
- 2017-11-08 CN CN201721483493.4U patent/CN207537439U/en not_active Withdrawn - After Issue
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603859A (en) * | 2017-11-08 | 2018-01-19 | 西安天隆科技有限公司 | Fully automatic integral nucleic acid extraction, amplification and detecting system |
| CN107603859B (en) * | 2017-11-08 | 2023-11-28 | 西安天隆科技有限公司 | Full-automatic integrated nucleic acid extraction, amplification and detection system |
| CN111607509A (en) * | 2019-02-22 | 2020-09-01 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN111607509B (en) * | 2019-02-22 | 2023-04-07 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN112439468A (en) * | 2019-08-30 | 2021-03-05 | 天津大学 | Rotary thermal circulation type multi-scale liquid drop digital polymerase chain reaction instrument system |
| CN111187718A (en) * | 2020-02-21 | 2020-05-22 | 厦门大学 | Nucleic acid extraction device and nucleic acid detection system |
| CN111187718B (en) * | 2020-02-21 | 2021-02-09 | 厦门大学 | Nucleic acid extraction device and nucleic acid detection system |
| CN113564044A (en) * | 2021-08-04 | 2021-10-29 | 圣湘生物科技股份有限公司 | Nucleic acid detection device and nucleic acid detection method |
| CN113564044B (en) * | 2021-08-04 | 2024-04-30 | 圣湘生物科技股份有限公司 | Nucleic acid detection device and nucleic acid detection method |
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