CN206229376U - A kind of centrifugal multi-channel fluid chip - Google Patents
A kind of centrifugal multi-channel fluid chip Download PDFInfo
- Publication number
- CN206229376U CN206229376U CN201620355456.4U CN201620355456U CN206229376U CN 206229376 U CN206229376 U CN 206229376U CN 201620355456 U CN201620355456 U CN 201620355456U CN 206229376 U CN206229376 U CN 206229376U
- Authority
- CN
- China
- Prior art keywords
- solution
- reaction tank
- chip
- reaction
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
Links
Abstract
The utility model discloses a kind of centrifugal multi-channel fluid chip, chip the first level is provided with first fluid external member to be included：First reaction tank, stream and solution inlet, the stream have the first solution working area for conveying liquid to above-mentioned first reaction tank；The stream includes first flow path and branch road, first flow path extends to first reaction tank and forms the first connectivity points from the valley, and the branch road forms the second connectivity points with first reaction tank and the second connectivity points extend to one outlet end towards the direction of pivot since then.The fluid device of the first level and the second aspect be able to can be interconnected by upper and lower interconnecting channels UNICOM, first fluid external member with second fluid external member.Microfluidic chip structure of the present utility model is ingenious, and the material and auxiliary element that can be reacted with different material in measuring samples or composition can be pre-loaded with differential responses device, is added by single or multiple solution and obtains reaction result.
Description
Technical field
The utility model is related to the sample analysis fluid chip and sample point that are used in being checked or analyzed etc. of biological respinse
The manufacture method of analysis method and sample analysis fluid chip.More particularly, it relates to can be used in the biology of albumen or DNA analysis
Chip (disposable biochip) and its manufacture method.
Background technology
In the past, for example in the biochemical reaction field of DNA reactions, albumen qualitative response etc., as treatment micro sample solution
Reaction unit, it is known have be referred to as micro-total analysis system (μ-TAS；TotalAnalysis System) or piece on laboratory
(Lab-on-Chip) technology.The technology is to provide (hereinafter also referred to " the reaction of multiple reative cells to a chip or reaction box
Pond ") or stream technology, multiple detection formations can be analyzed, or multiple reactions can be carried out.These technologies are by making
Chip and reaction box are minimized, it is possible thereby to reduce the usage amount of medicine, various advantages are considered to have always.
Patent document 1 (CN 101609088A) is disclosed in the presence of external electrical field, and electric field is applied to charged drop
Power, control particulate send stream device in microfluidic channel to what each stub area was moved.But, in the method, it is necessary to
The complex mechanism and equipment of generation electric field are introduced to chip；Again because conveying liquid needs to be first converted into drop in electric field region,
And stream is sent to designated area in the way of drop, greatly reduce the processing speed of sample.
Patent document 2 (CN 103055973A) is disclosed using the electroosmotic pump for driving sample, by powered different determinand
The device that matter is separate.But the method is only applicable to must be with electric charge sample, to general biological sample and uncharged
Sample does not work.
Patent document 3 (CN 102369443A) discloses a kind of centrifugal infusion chip, i.e. liquid depositing from chip center
Peripherad each well in storage portion carries out centrifugation dispatching.The design of the primary flow path, i.e. mountain portion are with valley with primary flow path wide
Design still fails to solve the problems, such as that each well matches somebody with somebody liquid uniformity；The design more wide than mountain portion of other valley, causes sample-adding process
Middle primary flow path has the problem that bubble is produced.Additionally, excessive solution is carried by adding waste liquid chamber, though realize to a certain extent
Each well distributes the uniformity of liquid volume, but in not ensuring that course of reaction liquid all the time full of well, and
Needs local surfaces modification is carried out inside microfluidic channel, i.e., the branch flow passage inner surface being connected with well it is hydrophilic
Treatment, the hydrophobic treatment of the branch flow passage inner surface being connected with the waste liquid chamber for collecting surplus solution, increased answering for chip manufacturing
Polygamy, processing cost and difficulty of processing.
Utility model content
The purpose of this part is to summarize some aspects of embodiment of the present utility model and briefly to introduce some preferable
Embodiment.May be done in this part and the description of the present application summary and utility model title a little simplified or omitted to keep away
Exempt to make the purpose of this part, specification digest and utility model title to obscure, and this simplification or omission cannot be used for limitation originally
The scope of utility model.
In view of problem present in above-mentioned and/or existing centrifugal multi-channel fluid chip and its method, it is proposed that this
Utility model.
Therefore, the one of purpose of the utility model is to provide a kind of sample analysis fluid core to well liquor charging
In piece, feeding method is simple, between each well liquid measure in the absence of deviation and the centrifugal multi-channel fluid core of low cost
Piece.
In order to solve the above technical problems, according to one side of the present utility model, the utility model provides following technology
Scheme：A kind of centrifugal multi-channel fluid chip, first fluid external member, the first fluid are provided with chip the first level
External member is by upper and lower two base materials with being combined into；First fluid external member includes multiple fluid devices：First reaction tank and the first reaction
The stream of pond connection and the solution inlet to stream injection solution, and by rotating the chip, solution is distributed to first anti-
Ying Chi, the stream has the first solution working area for conveying liquid to above-mentioned first reaction tank, and first solution is kept in
Area has a valley relative to pivot direction；The stream includes first flow path and branch road, and first flow path is described in
Valley extends to first reaction tank and forms the first connectivity points, and the branch road forms the second connectivity points with first reaction tank
And the second connectivity points extend to one outlet end towards the direction of pivot since then, the port of export can be independent, it is also possible to it
He merges stream device.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：The branch road
The second connectivity points are formed with first reaction tank and the second connectivity points extend to the first solution working area or first since then
Lead to the port of export after stream.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
Any one point of fluid device in fluid external member from the first solution working area and its backward, can be from the ground floor of the chip
Certain depth towards upper surface or lower surface extends, and upper and lower intercommunication stream is formed, by first fluid external member from the first of chip
Aspect sets or extends to the second aspect, and the gas and solution allowed in first fluid external member flow to the second aspect from the first level
Fluid device.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
The second reaction tank is also included in fluid external member, second reaction tank is arranged on the branch road.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
The first reaction warehouse, first reaction warehouse and first reaction tank or the second reaction tank or branch are also included in fluid external member
Road is connected, and first reaction warehouse is connected with gas vent.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
The second reaction warehouse is additionally provided between reaction warehouse and gas vent.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
Solution working area is for multiple and can connect each other, and solution enters into nearest first solution working area from solution inlet will
After it is full of, other the first solution working areas are flowed into successively, finally lead to solution injection refluxing opening.Solution working area is molten with described
Can be connected by storage pool between liquid injection refluxing opening.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
Solution working area is for multiple and can communicate with each other, and the first solution working area is connected by effluent road with aerating passage, is connected
Aerating passage form integral type connectivity structure, and with least one aerating hole.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：The solution
Working area is for multiple and can communicate with each other, and its port of export forms integral type connectivity structure by exhaust passage, final to have altogether
Same gas vent.
According to other side of the present utility model, there is provided following technical scheme：A kind of centrifugal multi-channel fluid
Chip, the ground floor or the second layer of the chip are provided with second fluid external member, include the second solution working area, connection second
Solution working area and with all or part of fluid device of identical in first fluid external member；Second fluid external member and first fluid
External member is connected.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：The reaction
In pond and reaction warehouse the test paper for showing biochemical reaction result, the test paper are provided with least one reaction tank or reaction warehouse
Including dry chemical test paper, Dry-type biochemical test paper or immune chromatography test paper.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Supporting setting
There is detecting instrument, it can be detected to the special spectrum signal that the solution in any reaction tank of chip is produced, and is detected
The analysis result of test sample product.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：Described first
Having on intercommunication stream can set the counterbore of hydration shell, and the air insulated in the runner can be caused air by the hydration shell
Can not pass through, and come when there is solution to flow through, solution can dissolve the hydration shell.The main component of the hydration shell is poly- second two
In alcohol, ethene-vinyl acetate polymer, polyacrylic acid, polyacrylamide shitosan, hyaluronic acid, polyvinyl alcohol, Sucrose products
One or several mixture.
As a kind of preferred scheme of centrifugal multi-channel fluid chip described in the utility model, wherein：When solution from
First solution working area (102) is driven when flowing into the first reaction tank (101) and the second reaction tank (106) by centrifugation, and at least one
Reaction tank can be filled.
The centrifugal multi-channel fluid chip provided using the utility model, as long as in chip production in differential responses
Different materials are pre-loaded with pond can just be carried out nucleic acid amplification reaction, biochemical reaction on the chip of same design, be immunized
The detection of the diversified forms such as reaction, or different materials are detected using same reaction formation, so as in a kind of chip platform
On realize various applications.For example, to detect the specific nucleic acid fragment in sample by nucleic acid amplification reaction on the chip
(the such as gene of certain mutated genes or pathogenic microorganism), can be pre-loaded with differential responses pond can be with measuring samples
There is the primer and auxiliary element of specific reaction in middle different nucleic acid fragments；To detect sample by biochemical reaction on chip
In predetermined substance or composition (such as blood sugar or triglycerides), can be pre-loaded with differential responses pond can be with measuring samples
There is the material and auxiliary element of specific biochemical reaction in middle different material or composition；To be examined by immune response on chip
Special component (such as certain antigen or antibody) in test sample product, can be pre-loaded with differential responses pond can be with measuring samples
There is the material and auxiliary element of specific immune response in middle different material or composition.
Innovative point of the present utility model be on reaction tank and reaction warehouse be provided with least two connectivity points respectively as
Solution gas enters the passage with outflow, its solution is easily full of, it is ensured that reaction is fully carried out.Solution enters fashionable, its gas
Flow pass can connect an independent gas outlet, it is to avoid solution and gas reverse flow on same stream.When molten
The solution in liquid working area is centrifuged when being assigned to fluid device, and it can in time obtain gas supplement balance pressure from measurement flow channel, more
The solution in it can be made to be evenly distributed to each fluid device.Upper and lower interconnecting channels can make reaction member be in different height not
Same aspect, the flow measurement that solution can be prevented unnecessary；It can be carried out suitably to the solution in the reaction member that is connected in different height
Supplement, it is ensured that the solution for partly carrying out the fluid device of biochemical reaction is full of；It also can be by the counterbore in upper and lower interconnecting channels
Set hydration shell carries out isolation closing to reacting gas or reacted constituent, but can be by open channel after aqueous dissolution, as one
Individual cleverly switching device.Simultaneously can be selectively opened due to being provided with extra steam vent, the first solution working area can be first
After distribution solution entering part fluid device is centrifuged, solution can be again added, the new solution for adding of centrifugation driving carries out hybrid reaction,
And enter more fluid devices.Second solution working area of second fluid external member can be additionally provided solution addition passage and
The reaction tank or reaction warehouse of its connection, allow the solution being injected separately into from the first solution working area and the second solution working area at certain
Reaction member converges mixing, there is provided more reaction conditions.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme of the utility model embodiment, below will be to being wanted needed for embodiment description
The accompanying drawing for using is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present utility model
Example, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these accompanying drawings
Obtain other accompanying drawings.Wherein：
Fig. 1 is the overlooking the structure diagram of centrifugal multi-channel fluid chip described in the utility model one embodiment；
Fig. 2 is liquid extruding when centrifugal multi-channel fluid chip is centrifuged described in the utility model embodiment illustrated in fig. 1
Air discharge flows to schematic diagram, and now the branch road is connected to the first flow path；
Fig. 3 is liquid extruding when centrifugal multi-channel fluid chip is centrifuged described in the utility model embodiment illustrated in fig. 1
Air discharge flows to schematic diagram, and now the branch road is connected to the solution working area；
Fig. 4 is liquid extruding when centrifugal multi-channel fluid chip is centrifuged described in the utility model embodiment illustrated in fig. 1
Air discharge flows to schematic diagram, and now the port of export is used as independent gas vent；
Fig. 5 is that the plan structure of centrifugal multi-channel fluid chip described in the utility model another embodiment is illustrated
Figure；
Fig. 6 illustrates for the plan structure of centrifugal multi-channel fluid chip described in the utility model further embodiment
Figure；
Fig. 7 illustrates for the plan structure of centrifugal multi-channel fluid chip described in the utility model a still further embodiment
Figure；
Fig. 8 illustrates for the plan structure of centrifugal multi-channel fluid chip described in the 5th embodiment of the utility model
Figure；
Fig. 9 illustrates for the plan structure of centrifugal multi-channel fluid chip described in the 6th embodiment of the utility model
Figure；
Figure 10 illustrates for the plan structure of centrifugal multi-channel fluid chip described in the 7th embodiment of the utility model
Figure；
Figure 11 is centrifugal multi-channel fluid chip top view part part described in the 8th embodiment of the utility model
Zoomed-in view, reaction tank phantom and aquation membrane structure schematic cross-sectional view；
Figure 12 is centrifugal multi-channel fluid chip perspective view part office described in the utility model embodiment illustrated in fig. 11
Portion's enlarged diagram；
Figure 13 illustrates for the plan structure of centrifugal multi-channel fluid chip described in the 9th embodiment of the utility model
Figure；
Figure 14 is the fragmentary perspective structure of centrifugal multi-channel fluid chip described in the tenth embodiment of the utility model
Schematic diagram；
Figure 15 is the fragmentary perspective knot of centrifugal multi-channel fluid chip described in the one embodiment of the utility model the tenth
Structure schematic diagram；
Figure 16 is the fragmentary perspective knot of centrifugal multi-channel fluid chip described in the one embodiment of the utility model the tenth
Structure schematic diagram；
Figure 17 is that the utility model is to lead with centrifugal multi-channel fluid chip middle and upper part base material described in above-described embodiment
The partial cutaway view schematic of hot material；
Figure 18 is the utility model and centrifugal multi-channel fluid chip middle and lower part base material covering described in above-described embodiment
The partial cutaway view schematic of Heat Conduction Material；
Figure 19 shows for the plan structure of centrifugal multi-channel fluid chip described in the 12nd embodiment of the utility model
It is intended to；
Figure 20 shows for the plan structure of centrifugal multi-channel fluid chip described in the 13rd embodiment of the utility model
It is intended to.
Specific embodiment
It is attached with reference to specification to enable above-mentioned purpose of the present utility model, feature and advantage more obvious understandable
Figure is described in detail to specific embodiment of the present utility model.
Many details are elaborated in the following description in order to fully understand the utility model, but this practicality is new
Type can also be different from other manner described here using other be implemented, and those skilled in the art can be without prejudice to this reality
Similar popularization is done with the case of new intension, therefore the utility model is not limited by following public specific embodiment.
Secondly, " one embodiment " or " embodiment " referred to herein refers to that may be included in the utility model at least one in fact
Special characteristic, structure or characteristic in existing mode." in one embodiment " that different places occur in this manual is not
Same embodiment is referred both to, nor the single or selective embodiment mutually exclusive with other embodiment.
Fig. 1 is the top view of the implementation method for representing the centrifugal multi-channel fluid chip of the utility model.This practicality
New chip has：Multiple first reaction tanks 101 on chip 100；And for defeated to each first reaction tank 101
Send solution, such as delivery reagent fluid (solution) stream.In order to liquid is transported into each the first reaction tank 101, stream
At least include a first solution working area 102 being connected with each first reaction tank 101, certainly, the first solution working area 102
It is connected with the first reaction tank 101 by first flow path 103.There is the inlet for injecting solution on stream.In the implementation of Fig. 1
In mode, with the solution inlet 105 being connected with the first solution working area 102.
Specifically, the first level of chip 100 is provided with first fluid external member, first fluid external member is by upper and lower two base materials
With being combined into；First fluid external member includes multiple fluid devices：First reaction tank 101 and the stream of the connection of the first reaction tank 101
With the solution inlet 105 that solution is injected to stream, and by rotating the chip 100, solution is distributed into the first reaction tank
101, stream has the first solution working area 102 for conveying liquid to above-mentioned first reaction tank 101, and first solution is kept in
Area 102 has a valley G relative to pivot direction；Stream includes first flow path 103 and branch road 104, first flow path
103 extend to the first reaction tank 101 from valley G forms the first connectivity points 103a, the formation of 104 and first reaction tank of branch road 101 the
Two connectivity points 104a and the second connectivity points 104a extends to one outlet end 115 towards the direction of pivot since then, the outlet
End can be independent, it is also possible to merges with other stream devices." the first level " described herein not emphasizes each fluid device
Depth is highly completely the same, and is that by centrifugal multi-channel fluid chip institute functional multiple fluid device
In an aspect of similar height.
Centrifugal multi-channel fluid chip of the present utility model, it is that the centrifugal force produced by rotating the chip will
Liquid distributes to the micro-fluid chip of each the first reaction tank 101, it is therefore preferable that for central portion has the point through rotary shaft
(hereinafter referred to as central point) it is discoid, but, as long as its be formed to relative to through chip rotary shaft rotation i.e.
Can, have no particular limits.If fruit chip is for discoid, then can be with its center as rotary shaft, and can be in the discoid chip
On with concentric circles configure the first reaction tank 101, thus effectively utilize space.In order to liquid is evenly distributed to the first reaction
Pond 101, it is very important to produce uniform centrifugal force, and chip can be designed in addition to inlet, is axle tool with central point
There is rotational symmetry, it is possible thereby to easily realize above-mentioned purpose.I.e., it is assumed that have N number of first reaction tank 101, then just form N number of right
Claim, thus can produce uniform centrifugal force.Certainly, want each first reaction tank 101 with liquid measure it is different when, then be not limited to
This.In addition, by with concentric circles configure the first reaction tank 101, thus, by rotary chip 100, can at one detection zone
Realize the analysis of all first reaction tanks 101.
First solution working area 102 is formed in than the first reaction tank 101 closer on the position of central point side.And,
In centrifugal multi-channel fluid chip of the present utility model, the first solution working area 102 is formed as relative to pivot
One valley G in direction.In addition, referring to have relative to central point direction most with the valley G relative to central point direction
A little louder.So, by between the first solution working area 102 have relative to central point direction a valley G, thus,
The liquid in the first solution working area 102 is injected into when chip rotates, naturally in the valley of the first solution working area 102
G is caught, therefore each the first solution working area 102 carries out appropriate solution and distributes big depending on the volume of its own to stream
Small, in order to the amount of solution for ensureing each the first reaction tank 101 is uniform, the volume in the first solution working area is also just designed as unification greatly
It is small.
In this embodiment, stream also includes first flow path 103 and branch road 104, and first flow path 103 is prolonged from valley G
Extend the first reaction tank 101 and form the first connectivity points 103a, and 104 and first reaction tank of branch road 101 forms the second connectivity points
104a and the second connectivity points 104a extends to first flow path 103 since then, it is of course also possible to the first solution working area 102 is extended to,
And ensure the first connectivity points 103a with the distance of pivot more than the second connectivity points 104a and the distance of pivot.By
This, referring to Fig. 2, during the centrifugal force for rotating the chip and producing, ensure that the liquid reagent in the first solution working area 102
(solution) is introduced into the first reaction tank 101 and full of the first reaction tank 101 through first flow path 103, now rotates and solution is produced
Raw centrifugal force will oppress gas in former first reaction tank 101 and branch road 104 and pass back into the by the second connectivity points 104a
Finally discharged by solution inlet 105 into the first solution working area 102 after one stream 103.In one embodiment, branch road
104 and the first reaction tank 101 form the second connectivity points 104a and the second connectivity points 104a extends to the first solution working area since then
102 or first flow path 103 after lead to the port of export 115.
Now, because liquid reagent (solution) amount in former first solution working area 102 is more than the appearance of the first reaction tank 101
Amount, then first flow path 103 and branch road 104 also acts as the effect of Buffer Pool, thus without each first reaction tank 101 of generation
The liquid reagent amount of interior distribution is inconsistent so as to cause the inconsistent phenomenon of reaction result in each first reaction tank 101, direct shadow
Ring the accuracy of reaction result.In this embodiment, because each first reaction tank 101 is each other independent, have no
Connection, so, unnecessary liquid will not cause the product in the first reaction tank 101 to phase in the first solution working area 102
Diffusion in adjacent first reaction tank, causes cross pollution.
The special construction of branch road 104 is set makes storage be filled up to after each corresponding solution centrifugal of the first reaction tank 101
First reaction tank 101 and first flow path 103 and/or branch road 104, can so make centrifugation distribute the uneven distribution for causing only
It is embodied on first flow path 103 and/or branch road 104, and ensures that the first reaction tank 101 is full up.
If the special construction without branch road 104 is set, it is assumed that even if liquid reagent is evenly distributed to when distribution is centrifuged
In each first reaction tank 101, but if when the reaction occurred in the first reaction tank 101 needs to carry out at a certain temperature,
Liquid reagent in first reaction tank 101 is caused liquid reagent amount in the first reaction tank 101 to reduce by thermal evaporation.Liquid reagent
The reduction of amount makes solution concentration in the first reaction tank 101 change, and liquid reagent is reduced in each first reaction tank 101
Amount is also not consistent.The change of reaction solution concentration and the reduction of the liquid reagent amount of the first reaction tank 101 can all influence reaction to tie
The accuracy of fruit.And after setting branch road 104 is communicated with first flow path 103 on the first reaction tank 101, even if there is the damage of amount of liquid
Consumption, the liquid reagent in first flow path 103 and/or branch road 104 can also be supplemented to the first reaction tank, made in the first reaction tank 101
Liquid reagent persistently keeps full up state.
Simultaneously as the first connectivity points 103a is more than the second connectivity points 104a with pivot with the distance of pivot
Distance, then when meaning centrifugation, the centrifugal force produced at the first connectivity points 103a will be greater than the centrifugation at the second connectivity points 104a
Power.Therefore, liquid reagent (solution) enters in the first reaction tank 101 the preferential first connectivity points 103a that passes through, and drains in it
Air, full of the first reaction tank 101, until reaction is fully completed.
In another embodiment, fluid device in first fluid external member from the first solution working area 102 backward
Any one point, can extend from the ground floor of the chip 100 to upper surface or lower surface certain depth, form mutually through-flow up and down
Road 114, first fluid external member is set from the first level of chip 100 or the second aspect is extended to, and allows in first fluid external member
Gas and solution the fluid device of the second aspect is flow to from the first level." the second aspect " described herein is also not emphasized
The depth of each fluid device is highly completely the same, and is that by centrifugal multi-channel fluid chip institute functional
Fluid device is in a complete aspect of similar height.The second reaction tank 106 is also included in first fluid external member, second is anti-
Pond 106 is answered to be arranged on branch road 104.The first reaction warehouse 107, the first reaction warehouse 107 and institute are also included in first fluid external member
State the first reaction tank 101 or the second reaction tank 106 or branch road 104 is connected, and the first reaction warehouse 107 and gas vent
109 are connected.The second reaction warehouse 108 is additionally provided between first reaction warehouse 107 and gas vent 109.First solution is kept in
Area 102 is for multiple and can connect each other, and solution enters into nearest first solution working area 102 from solution inlet 105
After being full of, other the first solution working areas 102 are flowed into successively, finally lead to solution injection refluxing opening 102a.
In this embodiment, the first solution working area 102 is for multiple and can communicate with each other, the first solution working area
102 are connected by effluent road 113 with aerating passage 110, and connected aerating passage 110 forms integral type connectivity structure, and tool
There is at least one aerating hole 110a.Institute's solution working area 102 is for multiple and can communicate with each other, and its port of export passes through exhaust passage
111 form integral type connectivity structure, finally with common gas vent 109.
As shown in Fig. 3~Figure 16, it illustrates another embodiment party of the centrifugal multi-channel fluid chip of the utility model
The top view of formula.This implementation method is with the difference of Fig. 1 illustrated embodiments：First reaction tank 101 and the second reaction tank
106 are connected.Certainly, the second reaction tank 106 is arranged on branch road 104, i.e., the position of the second reaction tank 106 is between the second connection
Between the connectivity points of point 104a and branch road 104 and first flow path 103.In this structure, the first reaction tank 101 is not changed
The first connectivity points 103a and the second connectivity points 104a position relationship, be always ensured that the first connectivity points 103a and pivot
Distance is more than the second connectivity points 104a and the distance of pivot.Therefore, referring to a upper implementation method, rotate the chip and produce
Centrifugal force when, ensure that the liquid reagent (solution) in the first solution working area 102 is introduced into first through first flow path 103
In reaction tank 101 and full of the first reaction tank 101, then enter back into the second reaction tank 106 and full of the second reaction tank 106,
Now rotating the centrifugal force produced to solution will oppress in former first reaction tank 101, the second reaction tank 106 and branch road 104
Gas is finally discharged into the first solution working area 102 by first flow path 103 by solution inlet 105, and herein, first is anti-
Pond 101 is answered to be used as buffer pool, the second reaction tank 102 can be by biochemical reaction tank.
Those skilled in the art should be able to know, the utility model two kinds of implementation methods listed herein, and it is covered
Only the first reaction tank 101 and there are two kinds of situations of the first reaction tank 101 and the second reaction tank 106 simultaneously.But, with
One reaction tank 101 and the second reaction tank 106 mean to be also possible to have the three, 4th etc. reaction tanks, similar structures and its change
Shape, should also cover within the protection domain of utility model at this end.
As shown in figure 4, chip of the present utility model has：Multiple first reaction tanks 101, second on chip 100
Reaction tank 106；And for reaction tank conveying solution, such as delivery reagent fluid (solution) stream.In order to by liquid
Each reaction tank is transported to, stream at least includes one be connected with each reaction tank first solution working area with valley G
102, first flow path 103 and branch road 104 also including the first solution working area 102 of connection and reaction tank.Have on stream and use
In the inlet of injection solution.In the embodiment illustrated in fig. 4, injected with the solution positioned at the end of the first solution working area 102
Mouthfuls 105 and positioned at end side, hold a concurrent post the gas vent 109 of air discharge.
In this embodiment, positioned at end side, hold a concurrent post air discharge gas vent 109 its actually with
The first reaction warehouse 107 being arranged on chip 100 is connected, and first reaction warehouse 107 is connected with the second reaction tank 106
It is logical, for depositing test strips.For example, in the nucleic acid amplification test experience of virus and bacteria, having been discharged from the second reaction tank 102
Into the reaction solution of amplification, into being provided with the first reaction warehouse of immune chromatography test paper, nucleic acid reaction liquid layer to be amplified is analysed
Nitrocellulose filter starts timing, and result is observed at 5 minutes：Detection T lines such as test paper have substantially red lines, illustrate the virus
The testing result of bacterium is the positive
Fig. 5 is that the plan structure for representing the utility model centrifugal multi-channel fluid chip in addition to the implementation is shown
It is intended to.Chip of the present utility model has：Multiple first reaction tanks 101, the second reaction tank 106 on chip 100；And
For to reaction tank conveying solution, such as delivery reagent fluid (solution) stream.In order to liquid is transported into each reaction
Pond, stream at least includes the first solution working area 102 with valley G being connected with each reaction tank, also including connection first
The first flow path 103 and branch road 104 of solution working area 102 and reaction tank.
In Fig. 7 this implementation method, the first solution working area 102 is connected on the chip 100 of the utility model chip
It is logical to form integral type connectivity structure, the port for injecting solution or gas liquid outflow is additionally provided with, such as have and be located at first
The solution of the solution inlet 105, the other end of the one end of solution working area 102 injects refluxing opening 102a and positioned at aerating
Aerating hole 110a on passage 110.Correspondence aerating hole 110a is also provided with aerating passage 110, in the first solution working area 102
Position between valley G and valley G is mountain portion S.Mountain portion S refers to herein, in the valley G and valley G in the first solution working area 102
Between be connected with the first solution working area 102 by effluent road 113 from the closest position aerating passage 110 of central point.
Aerating passage 110 is connected by effluent road 113 with the first solution working area 102, when chip is centrifuged, when valley G's
When solution orders about inflow first flow path 103 by centrifugal force, it is molten that aerating passage 110 can uniformly give first by effluent road 113
Liquid working area 102 carries out gas supplement, it is preferred that aerating passage 110 is formed as circular configuration.When the first solution working area 102
Inside fill solution without selection centrifugation carry out liquid distribute when, can enter to aerating passage 110 by from aerating hole 110a
Promoting the circulation of qi body injects, so as to be pressurizeed to the solution in each first solution working area 102 respectively, drives solution to enter first-class
Body 103.In this embodiment, it is connected by storage pool 112 between the first solution working area 102 and solution injection refluxing opening 102a
It is logical.First solution working area 102 is formed in than the first reaction tank 101 closer on the position of central point side.And, this practicality
In new centrifugal multi-channel fluid chip, the first solution working area 102 is formed as relative to pivot direction
One valley G.In addition, referring to that there is the maximum point relative to central point direction with the valley G relative to central point direction.
So, with a valley G relative to central point direction, thus, it is injected into by between the first solution working area 102
When chip rotates, naturally in the first solution working area 102, valley G is converged liquid to the first solution working area 102
Collection, thus can ensure each the first solution working area 102 carry out appropriate solution distribution to stream.First solution working area
102 could be arranged to waveform, and corrugated first solution working area 102 forms a circle.In the utility model, with
The different material using the detection of same reaction formation, example are pre-loaded with the connected reaction tank in one solution working area 102 respectively
Can be such as pre-loaded with differential responses pond can different from testing sample nucleic acid fragments occur the primer of specific reactions and
Auxiliary element, is centrifuged and nucleic acid amplification reaction by the sample introduction of multigroup sample, just can be on a chip to multigroup testing sample
In specific nucleic acid fragment detected.
In this embodiment, stream also includes first flow path 103 and branch road 104, and first flow path 103 is prolonged from valley G
Extend the first reaction tank 101 and form the first connectivity points 103a, and 104 and first reaction tank of branch road 101 forms the second connectivity points
104a and since then the second connectivity points 104a extend to first flow path 103, and ensure the first connectivity points 103a with pivot
Distance is more than the second connectivity points 104a and the distance of pivot.
The effluent road 113 of first solution working area 102 is connected with aerating passage 110.In this embodiment, the first reaction
Pond 101 is connected by first flow path 103 with the first solution working area 102, and the second reaction tank 106 is arranged on branch road 104.For
More preferable utilization space, as shown in figure 5, stream has direction between adjacent first reaction tank 101 with the second reaction warehouse 108
One crest in pivot direction, and be connected with exhaust passage 111.Here, crest refers to, the second reaction warehouse 108 is being connected
On from the closest position of central point.Exhaust passage 111 is configured to chip 100 in concentric circles, with gas vent 109
It is connected.Aerating passage 110 is also arranged to chip 100 in concentric circles, and its one end is provided with aerating hole 110a, and molten with first
Liquid working area 102 is connected.
Second reaction tank 106 is arranged on the branch road 104.In this embodiment, the second reaction tank 106 is directly with
Two reaction warehouses 108 are connected and connect exhaust passage 111.In order to preferably be centrifuged and carry out liquid distribution, branch road 104 with it is first-class
The connectivity points that road 103 is formed want optimization design with the second connectivity points 104a with the distance of pivot with the distance of pivot,
During the centrifugal force for rotating the chip and producing, ensure that the liquid reagent (solution) in the first solution working area 102 through first
Stream 103 is introduced into the first reaction tank 101 and full of the first reaction tank 101, then into the second reaction tank 106, is now revolved
The centrifugal force for turning to produce solution leads to the gas oppressed in former first reaction tank 101, the second reaction tank 106 and branch road 104
Cross first flow path 103 and flow back into the first solution working area 102 and finally discharged by solution inlet 105.
As shown in fig. 6, it is with the difference of embodiment illustrated in fig. 5, the first reaction warehouse 107 is provided with chip 100.
First reaction warehouse 107 is connected between the second reaction tank 106 and the second reaction warehouse 108.
Fig. 7 and Fig. 8 illustrated embodiments, can be seen as the deformation for embodiment illustrated in fig. 6, but be not limited thereto.
As schemed, effluent road 113 is omitted herein.That is, when being centrifuged, valley G is necessary, thus, is injected into first
When chip rotates, naturally in the first solution working area 102, valley G is caught the liquid in solution working area 102, therefore
Each the first solution working area 102 can be ensured carries out appropriate solution distribution to stream.
Centrifugal multi-channel fluid chip part before the embodiment that Figure 11~Figure 20 is provided is different from is that it is built
The connection between different aspects or different height or the fluid device of different surfaces is stood.In this embodiment, it is centrifugal
Multi-channel fluid chip, the first level of chip 100 or the second aspect are provided with second fluid external member, include the second solution
Working area, connection the second solution working area and with all or part of fluid device of identical in first fluid external member；Second
Body external member is connected with first fluid external member, and leads to a common gas vent 109.In reaction tank and reaction warehouse extremely
The test paper of display biochemical reaction result is provided with a few reaction tank or reaction warehouse, the test paper is tried including dry chemical
Paper, Dry-type biochemical test paper or immune chromatography test paper.It is preferred that supporting in centrifugal multi-channel fluid chip be provided with detector
Device, it can be detected to the special spectrum signal that the solution in any reaction tank of chip 100 is produced, and obtain being detected sample
Analysis result.
Such as Figure 15 and Figure 16, on the basis of such as the embodiment of Fig. 4, multiple branch roads 104 by multiple intercommunication streams 114 with it is another
An exhaust passage UNICOM on one layer, the unified port of export 115 of the exhaust passage UNICOM.
Referring to Figure 11 and Figure 12, it is provided with being formed the counterbore 120 of hydration shell, the aquation on the first intercommunication stream 114
Air insulated in runner can be prevented air from passing through by film, and be come when there is solution to flow through, and solution can dissolve hydration shell.
The main component of hydration shell is polyethylene glycol, ethene-vinyl acetate polymer, polyacrylic acid, polyacrylamide shitosan, transparent
One or several mixture in the products such as matter acid, polyvinyl alcohol, sucrose.
Centrifugal multi-channel fluid chip has：The chip 100 of reaction tank, hand-hole and stream is formed with, it passes through
Be engaged or fit the base material composition for setting up and down.As base material, as long as do not influence the base material of reagent just not limit particularly
System, particularly, if using containing the resin material of any one in polypropylene, makrolon, acrylic acid, be able to ensure that good
Good visible light permeability.As polypropylene, it is possible to use the random copolymer of homo-polypropylene or polypropylene and polyethylene.Separately
Outward, as acrylic acid, it is possible to use polymethyl methacrylate or methyl methacrylate and other methyl can be used
The EVA of the monomers such as acrylate, acrylate, styrene.In addition, in the case of using these resin materials, it is also possible to really
Protect the heat resistance and intensity of chip.As the material in addition to resin material, the gold of aluminium, copper, silver, nickel, brass, gold etc. can be enumerated
Category material.It is also excellent in terms of pyroconductivity and sealing property during using metal material.And, by making reaction tank at least
The holding of side base material is transparent, detection and analysis thus, it is possible to carry out fluorescence etc. from outside.Wherein, in the utility model
" transparent " and " photopermeability " refers to, the mean transmissivity under the wavelength region of detection light is more than 70% situation.If
Using the photopermeability material of visible region (350~780nm of wavelength), then the reagent state in chip is easily recognized, but simultaneously
It is not limited to this.
As the processing method of the base material for forming well, stream and waste liquid portion, when for resin material, can be using note
It is moulded various resin molding methods or machine cuts of type, vacuum forming etc. etc..When for metal material, can be by implementing to utilize
The attrition process of thicker base material or etching, formed compared with pressure processing or stretch process is implemented on foil.In addition, conduct
Chip 100, during especially with containing the resin material of any one in polypropylene, makrolon, acrylic acid, it can be ensured that good
Photopermeability well, heat resistance and intensity.In addition, when the thickness of base material is 50 μm~3mm scopes, it can be ensured that good light
Permeability, heat resistance and intensity, and can reliably carry out recess processing.In addition, when the thickness of base material is 10 μm~300 μm models
When enclosing, the heat conductivity and sealing of base material can be simultaneously met.
As laminating base material method, can enumerate and be provided as the resinous coat of adhesive linkage on a base material, and melt it
Melt and be bonded two methods of base material.Preferred resin coating is arranged on pyroconductivity metal material base material high and is melted
Bonding.As the material of resinous coat, it is possible to use the resin material of PET, polyacetals, polyester or polypropylene etc..Certainly, may be used also
With by the way of adhesive tape is fixed and paraffin binds.
In above-mentioned applying method, the light for easily carrying out microfabrication and suitable fluorescence measurement is used preferably as base material
Permeability resin material, is used pyroconductivity high and easily sets resinous coat and pasted by melting adhered as base material
The metal material of conjunction.In addition, forming resinous coat by metallic substrate surface, Metal Substrate can not be considered in selected material
The resistance to chemical reagents of material itself.
In addition, substrate surface formed resinous coat when, by as resinous coat bottom formed thickening (anchoring,
Anchor) layer, thus can use a laser to carry out melting adhered.The carbon black for absorbing optical maser wavelength light is mixed with adhesion promoting layer
(light absorption material), heat is produced by irradiating laser, melting adhered thus, it is possible to be carried out to resinous coat.Or, it is also possible to
Adding carbon black in resinous coat or the surface of resinous coat being applied to by way of black come instead of adding in adhesion promoting layer
Plus carbon black.For example, in the light of the infra-red laser diode that illumination wavelength is 900nm or so, it is also possible to which effective ground molten resin is applied
Layer.Different from thermal welding, laser welding need not heat chip, therefore, can hardly have influence on chip or be fixed on core
In the case of reagent on piece, implement the laminating of base material.
During practical operation, first it is added dropwise respectively with solution such as nucleic acid primer and amplification enzymes, by core in the second reaction tank 106
Piece is dried, and obtains primer and amplification enzyme of solid-state etc., and nucleic acid immunization chromatograph test strip is placed in the first reaction warehouse 107, will
The base material 200 of chip 100 and second is fitted, and blend compounds band or other articles seal the He of gas vent 109, by solution inlet
105 injections are with nucleic acid-templated solution to be detected and are gradually filled with the first solution working area 102, now, such as Fig. 5~Fig. 8 institutes
Show embodiment, solution injects the gases that act as discharge first solution working area 102 in of the refluxing opening 102a in whole process, if
It is that injection solution is excessive, unnecessary meeting can be assigned to storage pool in centrifugation.Whole chip is placed on centrifuge at centrifugation
Reason, using centrifugal force, the first reaction tank 101 and the is flowed into the first solution working area 102 successively with nucleic acid-templated solution
Two reaction tanks 106, with nucleic acid-templated solution by dry primer and amplification enzyme dissolving mixing, chip are placed on certain
After carrying out nucleic acid amplification reaction under temperature conditionss, dilute solution is injected into the first solution working area 102, open gas vent
109, gas can be injected from aerating hole 110a, pressure will be delivered to the solution working area of side runner 113 and first from aerating passage 110
102, because the grade fluid device of the first reaction tank 101 is connected to what is connect with gas vent 109, dilute solution will be anti-into second
Answer pond 106 and the nucleic acid solution of amplification is diluted and extruded in the test strips for flowing into the first reaction warehouse 107, test strips reaction display
As a result.
In addition it is also possible to dilute solution is injected into the first solution working area 102, it is molten by what is injected by centrifugal chip disk
Liquid is driven into each fluid device, and be diluted for the nucleic acid solution for entering the second reaction tank 106 and will expand by dilute solution
And extruding is flowed into the test strips of the second reaction warehouse 108, test strips reaction display result.
Further, it is also possible to dilute solution is injected into the second solution working area, by centrifugal chip disk, the solution drive that will be injected
Dynamic to enter each fluid device, the nucleic acid solution for entering the second reaction tank 106 and will expand is diluted and squeezed by dilute solution
Pressure is flowed into the test strips of the first reaction warehouse 107, test strips reaction display result.
Further, it is also possible to dilute solution is injected into first solution working area the second solution working area simultaneously, by the way that core is centrifuged
Piece disk, each fluid device, the core that dilute solution will enter the second reaction tank 106 and will expand are driven into by the solution of injection
Acid solution is diluted and extrudes in the test strips for flowing into the first reaction warehouse 107, test strips reaction display result.
In three kinds of dilutions injection case more than, in addition to nucleic acid primer and amplification enzyme, if in first fluid external member
The second reaction tank in be previously added the antibody of immune response, then the chip disk can be used to carry out the examination such as Immunofluorescence test
Test.If being previously added the correlated response composition of biochemical reaction in the second reaction tank of first fluid external member, the chip disk can
For carrying out various biochemical branches.
Correspondingly, if the second solution working area in second fluid external member is also communicated with reaction tank or reaction warehouse, also exist
In one of reaction tank or reaction warehouse be provided with the above nucleic acid reaction, immune response, the correlated response composition of biochemical reaction,
Then after dilute solution is injected in first solution working area the second solution working area simultaneously, dilute solution can order about first fluid set
Collaborate after the part liquid the outflow how reaction of second fluid external member is completed, such that it is able to enter in follow-up reaction tank or reaction warehouse
Single step reaction.
The correspondingly nucleic acid reaction in chip disk, immune response, the result of biochemical reaction can be by way of test strips
Naked eyes color interpretation is carried out, or the solution of certain reaction tank or reaction warehouse that are provided with transparent base is entered by spectral instrument
Row spectrum interpretation.
As shown in figure 19, on chip 100 multiple first reaction tanks 101 and for each the first reaction tank
101 conveying solution, such as delivery reagent fluid (solution) stream.In order to liquid is transported into each the first reaction tank 101,
Stream at least includes a first solution working area 102 being connected with each first reaction tank 101, and certainly, the first solution is kept in
Area 102 is connected by first flow path 103 with the first reaction tank 101.The side of first reaction tank 101 and the UNICOM of gas vent 109,
There is the inlet for injecting solution on stream.The liquid reagent (solution) of injection to the first solution working area 102, by from
The heart is moved, and liquid reagent (solution) enters the first reaction tank 101, the reaction tank of first flow path 103 and first by first flow path 103
101 air is discharged through gas vent 109, and test strips are housed in the first reaction tank 101, and it is (molten that test strips absorb liquid reagent
Liquid), test strips reaction display result.
As shown in figure 20, it is that each the head and the tail UNICOM of the first solution working area 102 in embodiment described in Figure 19 gets up to have
The inlet of unified injection solution, each side UNICOM of the first reaction tank 101 there is into unified gas vent 109 to one
Exhausting loop embodiment, exhausting loop can be arranged on the front of chip 100, it is also possible in reverse side, as long as from unified note
Entrance injects liquid reagent (solution), and by centrifugal motion, liquid reagent (solution) enters all of first by first flow path 103
Reaction tank 101, the air of the reaction tank 101 of all of first flow path 103 and first is discharged through unified gas vent 109, the
Test strips are housed, test strips absorb liquid reagent (solution), test strips reaction display result in one reaction tank 101.
An implementation method as shown in Figure 11,12, it is another with respect to the first reaction tank 101 that the second reaction tank 106 is arranged on chip
One aspect, the second reaction tank 106 passes through intercommunication stream 114 and the UNICOM of branch road 104, when completion centrifugally operated, the first reaction tank 101
During with the second 106 hydraulically full reagent (solution) of reaction tank, when the first reaction tank 101 keeps flat upward, because UNICOM second reacts
Pond 106 is all the intercommunication stream 114 of vertical direction, therefore the liquid reagent being in the second reaction tank 106 of low level aspect is (molten
Liquid) because of gravity or will not rock, and pass through the diffusion flow measurement of branch road 104.
When chip is heated, the liquid reagent (solution) in the second reaction tank 106 can be because of the gas diffusion after evaporation
Tailed off to branch road 104 and its other fluid devices of connection, because the first reaction tank 101 is higher in the second reaction tank 106
Horizontal level, so liquid reagent (solution) in the first reaction tank 101 can because the reason such as gravity suitably to flow into second anti-
Answer and supplemented in pond 106, so as to make the second reaction tank be kept flat in chip and remain that liquid is full of in course of reaction, no
Can influence to show reaction result because the liquid reagent (solution) in the second reaction tank 106 is reduced.
There is intercommunication stream 114 at branch road 104 1, there is counterbore 120 on intercommunication stream 114, the bottom of counterbore 120 is by mutual
Through-flow road 114 and the UNICOM of branch road 104, the top of counterbore 120 and the UNICOM of the first reaction warehouse 107, add main component to be in counterbore 120
Polyethylene glycol, ethene-vinyl acetate polymer, polyacrylic acid, polyacrylamide shitosan, hyaluronic acid, polyvinyl alcohol, sucrose
Deng one or several the mixture in product, hydration shell 120a, aquation under this state are formed in the bottom of counterbore 120 after drying
Film 120a planes are be arranged in parallel with chip plane, additionally, hydration shell 120a planes can also be vertically arranged with chip plane, i.e.,
Gas cut-off effect is played to branch road 104 without intercommunication stream 114.
, when no liquid reagent (solution) comes, playing can be by the first reaction warehouse 107 and the second reaction tank for hydration shell 120a
Isolation is closed between 106, prevents reacting gas or reacted constituent flow effect experimental result, when liquid reagent (solution) stream
When to hydration shell 120a, hydration shell 120a dissolvings, liquid reagent (solution) can flow to the first reaction warehouse from the second reaction tank 106
In 107, test strips absorber reagent (solution), test strips reaction display result.
As shown in figure 13, it is by each the head and the tail UNICOM of the first solution working area 102 in embodiment described in Figure 11,12
There is the inlet of unified injection solution, there is into unified gas vent in each side UNICOM of the first reaction warehouse 107 to one
The embodiment of 109 exhausting loop, and in the (not shown) of branch road 104 by the (not shown) of intercommunication stream 114 and the
Two solution working area (102) UNICOMs, second solution working area (102) (not shown) both can again may be used as degassing function
To inject second liquid reagent (solution).
When primer and amplification enzyme that PCR nucleic acid amplifications are in advance placed in the second reaction tank 106, be there is also in solution and treated
Amplification it is nucleic acid-templated when, the second reaction tank 106 can occur complete PCR nucleic acid amplification reactions.When the first solution working area is dilute
Release after liquid is driven into the second reaction tank 106 by centrifugal force, pcr amplification reaction solution will be into the first reaction warehouse 107, by examination
Paper slip shows reaction result.
If be configured with appropriate spectral detection instrument, the expansion of performing PCR nucleic acid can be directly entered to the second reaction tank 106
Increase the signal detection of reaction, be analyzed by instrument and draw reaction result.
If the lower surface of the second reaction tank 106 is not configured to Heat Conduction Material, chip can be integrally placed to specifically
Constant temperature nucleic acid amplification is carried out in temperature environment, by being that test strips or spectral instrument carry out reaction result interpretation.
It should be noted that above example is only used to illustrate the technical solution of the utility model and unrestricted, although reference
Preferred embodiment has been described in detail to the utility model, it will be understood by those within the art that, can be to this reality
Modified with new technical scheme or equivalent, without deviating from the spirit and scope of technical solutions of the utility model,
It all should cover in the middle of right of the present utility model.
Claims (15)
1. a kind of centrifugal multi-channel fluid chip, first fluid device, described are provided with chip (100) the first level
One fluid device is by upper and lower two base materials with being combined into；First fluid device includes multiple fluid devices：First reaction tank
(101) and the first reaction tank (101) connection stream and to stream inject solution solution inlet (105), and by rotation
The chip (100), the first reaction tank (101) is distributed to by solution, it is characterised in that：
The stream has the first solution working area (102) for conveying liquid to above-mentioned first reaction tank (101), and this first
Solution working area (102) is with a valley (G) relative to pivot direction；
The stream includes first flow path (103) and branch road (104), and first flow path (103) extends to described from the valley (G)
First reaction tank (101) forms the first connectivity points (103a), and the branch road (104) and first reaction tank (101) form the
Two connectivity points (104a) and the second connectivity points (104a) extend to one outlet end (115) towards the direction of pivot since then.
2. centrifugal multi-channel fluid chip as claimed in claim 1, it is characterised in that：The branch road (104) with it is described
It is temporary that first reaction tank (101) the second connectivity points of formation (104a) and since then the second connectivity points (104a) extend to first solution
Lead to the port of export (115) after depositing area (102) or first flow path (103).
3. centrifugal multi-channel fluid chip as claimed in claim 1 or 2, it is characterised in that：The first fluid device
In fluid device from the first solution working area (102) and its backward any one point, can be from the first of the chip (100)
Aspect extends to the certain depth of upper surface or lower surface, upper and lower intercommunication stream (114) is formed, by first fluid device from chip
(100) the first level sets or extends to the second aspect, and the gas that allows in first fluid device and solution are from ground floor surface current
Move the fluid device to the second aspect.
4. centrifugal multi-channel fluid chip as claimed in claim 1 or 2, it is characterised in that：The first fluid device
On also include the second reaction tank (106), second reaction tank (106) is arranged on the branch road (104).
5. centrifugal multi-channel fluid chip as claimed in claim 4, it is characterised in that：On the first fluid device also
Include the first reaction warehouse (107), first reaction warehouse (107) and first reaction tank (101) or the second reaction tank
Or branch road (104) is connected, and first reaction warehouse (107) is connected with gas vent (109) (106).
6. centrifugal multi-channel fluid chip as claimed in claim 5, it is characterised in that：First reaction warehouse (107)
The second reaction warehouse (108) is additionally provided between gas vent (109).
7. the centrifugal multi-channel fluid chip as described in claim 1,2,4~6 is any, it is characterised in that：Described first
Solution working area (102) is for multiple and can connect each other, and solution enters into nearest first from solution inlet (105)
Behind solution working area (102), other the first solution working areas (102) are flowed into successively, finally lead to solution injection refluxing opening
(102a)。
8. centrifugal multi-channel fluid chip as claimed in claim 7, it is characterised in that：The first solution working area
(102) for multiple and can communicate with each other, the first solution working area (102) is with the mountain portion for being opposite in pivot direction
(S), and each mountain portion (S) is connected by effluent road (113) with aerating passage (110), connected aerating passage (110) is formed
Integral type connectivity structure, and with least one aerating hole (110a).
9. centrifugal multi-channel fluid chip as claimed in claim 7, it is characterised in that：The solution working area (102)
For multiple and can communicate with each other, and integral type connectivity structure is formed by exhaust passage (111), finally with common gas
Steam vent (109).
10. a kind of centrifugal multi-channel fluid chip as claimed in claim 3, it is characterised in that：The first of the chip
Aspect or the second aspect are provided with second fluid device and include the second solution working area, the second solution working area of connection and with the
The all or part of fluid device of identical in one fluid device；Second fluid device is connected with first fluid device.
The 11. centrifugal multi-channel fluid chip as described in claim 1,2,4~6,8~10 is any, it is characterised in that：Institute
State the test paper that display biochemical reaction result is provided with least one reaction tank in reaction tank and reaction warehouse or reaction warehouse, institute
Stating test paper includes dry chemical test paper, Dry-type biochemical test paper or immune chromatography test paper.
The 12. centrifugal multi-channel fluid chip as described in claim 1,2,4~6,8~10 is any, it is characterised in that：Match somebody with somebody
Set is provided with detecting instrument, and it can be carried out to the special spectrum signal that the solution in some reaction tank of chip (100) is produced
Detection, obtains being detected the analysis result of sample.
13. centrifugal multi-channel fluid chips as claimed in claim 3, it is characterised in that：Described about first is mutually through-flow
Having on road (114) can set the counterbore (120) of hydration shell, and the hydration shell can cause the air insulated in the runner
Air can not pass through, and come when there is solution to flow through, and solution can dissolve the hydration shell.
14. centrifugal multi-channel fluid chips as claimed in claim 13, it is characterised in that：The hydration shell it is main into
Point be polyethylene glycol, ethene-vinyl acetate polymer, polyacrylic acid, polyacrylamide shitosan, hyaluronic acid, polyvinyl alcohol,
One or several mixture in Sucrose products.
A kind of 15. centrifugal multi-channel fluid chips that can be heated, it is characterised in that：With structure for example claim 1,2,
Any described centrifugal multi-channel fluid chip in 4~6,8~10,13 or 14；
The all or part of stream of the identical of reaction tank or reaction warehouse or second fluid device in the first fluid device
At least one base material belongs to Heat Conduction Material in the base material up and down of body device.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2016200665546 | 2016-01-22 | ||
| CN201620066554 | 2016-01-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206229376U true CN206229376U (en) | 2017-06-09 |
Family
ID=58985802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620355456.4U Withdrawn - After Issue CN206229376U (en) | 2016-01-22 | 2016-04-25 | A kind of centrifugal multi-channel fluid chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206229376U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105964313A (en) * | 2016-01-22 | 2016-09-28 | 上海快灵生物科技有限公司 | Centrifugal type multi-channel microfluidic chip |
| CN108642141A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection reagent mixing adding set |
| CN110508337A (en) * | 2019-03-27 | 2019-11-29 | 广州万孚生物技术股份有限公司 | Vitro detection device and its loading mechanism |
-
2016
- 2016-04-25 CN CN201620355456.4U patent/CN206229376U/en not_active Withdrawn - After Issue
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105964313A (en) * | 2016-01-22 | 2016-09-28 | 上海快灵生物科技有限公司 | Centrifugal type multi-channel microfluidic chip |
| CN105964313B (en) * | 2016-01-22 | 2019-04-09 | 上海快灵生物科技有限公司 | A kind of centrifugal multi-channel fluid chip |
| CN108642141A (en) * | 2018-06-07 | 2018-10-12 | 国家纳米科学中心 | Nucleic acid detection reagent mixing adding set |
| CN108642141B (en) * | 2018-06-07 | 2021-09-10 | 国家纳米科学中心 | Nucleic acid detection reagent mixing and adding device |
| CN110508337A (en) * | 2019-03-27 | 2019-11-29 | 广州万孚生物技术股份有限公司 | Vitro detection device and its loading mechanism |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105964313B (en) | A kind of centrifugal multi-channel fluid chip | |
| CN110142066A (en) | Micro-fluidic chip and analysis system | |
| US9897596B2 (en) | Microfluidic disc for use in with bead-based immunoassays | |
| JP5392369B2 (en) | Sample analysis chip, sample analysis apparatus using the same, sample analysis method and gene analysis method, and sample analysis chip manufacturing method | |
| KR20110088746A (en) | Centrifugal micro-fluidic device and method for detecting analytes from liquid specimen | |
| CN206229376U (en) | A kind of centrifugal multi-channel fluid chip | |
| AU756710B2 (en) | Apparatus for, and method of, varying the rate of flow of fluid along a pathway | |
| CN109999931A (en) | Chemiluminescence detection micro-fluidic chip and application method, reagent cleaning method | |
| CN104360091B (en) | A kind of chip, using method and purposes | |
| CN106513063A (en) | Centrifugal chip capable of achieving sequential reactions and mixing method thereof | |
| CN205797240U (en) | A kind of integrated micro-flow control chip | |
| CN102788780B (en) | Microfluidic chip for biological chemiluminescence detection and manufacturing method thereof | |
| US20090155125A1 (en) | Microchip | |
| CN111073810A (en) | Microfluidic chip, system and method integrating nucleic acid extraction, amplification and detection | |
| CN106947683A (en) | A kind of nucleic acid extraction purification devices and method | |
| CN209923319U (en) | Microfluidic chip structure and analysis device | |
| TW200931018A (en) | Reaction chip and method of manufacturing the same | |
| CN107513495A (en) | Multichannel droplet detection chip for detection of nucleic acids | |
| CN108148744A (en) | A kind of drop number pcr chip and corresponding method of detection and detecting system | |
| CN113649095B (en) | Highly integrated microfluidic chip for nucleic acid detection and use method | |
| CN211921576U (en) | Micro-fluidic chip and system integrating nucleic acid extraction, amplification and detection | |
| CN215087246U (en) | Multifunctional microfluidic detection chip | |
| CN206814737U (en) | A kind of nucleic acid extraction purification devices | |
| CN114113642A (en) | Detection kit and method for performing coagulation analysis by using microfluidic technology | |
| CN112375669A (en) | Micro-fluidic chip for extracting, purifying and detecting nucleic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170609 Effective date of abandoning: 20190409 |