CN113980776A - Cartridge, sample processing device, and molecular diagnostic system - Google Patents
Cartridge, sample processing device, and molecular diagnostic system Download PDFInfo
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- CN113980776A CN113980776A CN202111480621.0A CN202111480621A CN113980776A CN 113980776 A CN113980776 A CN 113980776A CN 202111480621 A CN202111480621 A CN 202111480621A CN 113980776 A CN113980776 A CN 113980776A
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- 238000012545 processing Methods 0.000 title claims abstract description 17
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- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 54
- 238000001514 detection method Methods 0.000 claims abstract description 15
- 239000011324 bead Substances 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims description 86
- 238000012546 transfer Methods 0.000 claims description 21
- 108020004707 nucleic acids Proteins 0.000 claims description 11
- 150000007523 nucleic acids Chemical class 0.000 claims description 11
- 102000039446 nucleic acids Human genes 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 6
- 238000009434 installation Methods 0.000 claims description 6
- 239000010409 thin film Substances 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000002699 waste material Substances 0.000 abstract description 28
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000000338 in vitro Methods 0.000 abstract description 2
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- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 238000001125 extrusion Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
The present application relates to the field of microfluidic control technology for in vitro diagnostics, and more particularly to a cartridge, a sample processing device and a molecular diagnostic system; a card box is applied to a rapid molecular detection system and comprises a card box body and an end cover buckled on the card box body; a first pipetting cavity for placing a main pipetting valve, a second pipetting cavity for placing an auxiliary pipetting valve, a sample cavity for storing a sample, a redissolution cavity for storing freeze-dried beads, a waste liquid cavity for storing waste liquid and a plurality of reagent cavities are formed in the cartridge body; the end cover can cover the waste liquid cavity for storing waste liquid, so that the waste liquid in the waste liquid cavity is prevented from volatilizing, and the environment is prevented from being influenced; the end cover can cover the sample cavity for storing the sample, and after the sample is filled into the sample cavity, the end cover seals the sample cavity, so that the sample can be prevented from being polluted, and the detection accuracy is improved.
Description
Technical Field
The present application relates to the field of microfluidic control technology for in vitro diagnostics, and more particularly, to a cartridge, a sample processing device, and a molecular diagnostic system.
Background
With the shift of medical treatment modes and the continuous development of individual medicine, molecular detection technology is widely applied, which can accurately detect diseases once symptoms, signs and biochemical changes occur or not even before the diseases occur by analyzing the tissue cells, hair, anticoagulation or dry blood traces of the detected people, genes and expression products thereof in formaldehyde-fixed paraffin-embedded tissues and completing nucleic acid (DNA and RNA) detection from the molecular level.
In the molecular detection technology, the extraction of nucleic acid (DNA and RNA) is usually completed in a cassette; however, the current card box is easy to cause pollution in the transportation and operation processes, so that the detection result has errors.
Disclosure of Invention
The application aims to provide a card box, a sample processing device and a molecular diagnosis system, which can solve the technical problem that the detection result has errors due to the pollution of the card box in the operation and transportation processes in the prior art to a certain extent.
The application provides a card box which is applied to a rapid molecular detection system and comprises a card box body and an end cover buckled on the card box body;
a plurality of chambers are formed in the cartridge body;
the end cap is capable of sealing at least one of the plurality of chambers and is capable of communicating chambers of the plurality of chambers other than the sealed chamber with the cartridge.
In the above technical solution, further, a sealing member is disposed at an open end of at least one of the chambers sealed by the end cap;
the sealing member has an operating portion and a sealing portion connected to the operating portion;
when the operation part is driven along a first direction, the sealing part can be opened or covered at the open end;
when the operation portion is driven in a second direction, the seal portion can be locked or unlocked to the open end.
In the above technical solution, further, the sealing member includes a chamber cover capable of serving as the sealing portion;
a handle capable of serving as the operating part is formed at one end of the chamber cover, and the handle is positioned on one side plate surface of the chamber cover facing the end cover; when the handle is driven along the first direction, the chamber cover can be opened or covered at the open end.
In the above technical solution, further, a limiting shaft is formed at the other end of the chamber cover, and the limiting shaft is located on a side plate surface of the chamber cover facing the chamber; the side wall of the cavity is provided with a limiting groove, and the limiting shaft can extend into the limiting groove;
the retainer shaft of the chamber lid is rotatable within the retainer slot when the handle is driven in the first direction.
In the above technical solution, further, a guide hole is formed on the end cap, and the handle can pass through the end cap through the guide hole;
the end cover is provided with a first sample inlet, and when the cavity cover is opened at the open end by driving the handle, the first sample inlet is communicated with the cavity.
In the above technical solution, further, the sealing member further includes a sealing cover located between the chamber cover and the open end;
a second sample inlet which is positioned on the same straight line with the first sample inlet is formed on the sealing cover;
when the chamber cover is opened at the open end, the first sample inlet is communicated with the second sample inlet.
In the above technical solution, further, the guide hole includes a first guide portion and a second guide portion;
the handle can move from the first end of the first guide part to the second end of the first guide part along the first direction so as to enable the chamber cover to rotate around the limiting shaft by a preset angle; when the handle is located at the second end of the first guide portion, the chamber is opened;
the first end of the second guide part is communicated with the first end of the first guide part, and the second end of the second guide part extends along the second direction; the handle can move to the second end of second guide part along the second direction via the first end of first guide part, and when the handle moved to the second end of second guide part, spacing axle by the first end of spacing groove moves to the second end of spacing groove, so that the chamber lid locks in the open end.
In the above technical solution, further, a sealing layer is disposed on the top of the chamber, which is partially communicated with the outside of the cartridge, so that the chamber forms a sealed chamber.
In the above technical solution, further, one of the chambers is a first pipetting cavity, at least one of the chambers is a reagent cavity, a liquid taking channel is arranged at the bottom of the first pipetting cavity, and the first pipetting cavity can be communicated with the reagent cavity through the liquid taking channel;
the cartridge further comprises a pipetting valve; one end of the liquid transferring valve can be communicated with the first liquid transferring cavity.
In the above technical solution, further, the pipetting valve includes a sealing member and a second valve body connected to the first pipetting cavity;
the sealing member is connected with the second valve body, and the second valve body is sealed against the first pipetting cavity through the sealing member;
a liquid taking hole and a conducting flow passage communicated with the liquid taking hole are formed at the bottom of the second valve body; the sealing element is provided with a conducting hole communicated with the conducting flow channel;
when the second valve body rotates in the first pipetting cavity, the sealing element moves synchronously with the second valve body, and the liquid taking hole is communicated with the liquid taking flow channel through the communicating flow channel and the communicating hole in sequence.
In the above technical solution, further, one end of the conducting flow passage is communicated with the liquid taking hole, and the other end extends along a straight line direction where the liquid taking hole passes through a circle center of the second valve body.
In the above technical solution, further, the liquid taking channels are provided in plurality, and the plurality of liquid taking channels are arranged at intervals along the circumferential direction;
when the second valve body and the sealing piece synchronously rotate in the first liquid transferring cavity, the liquid taking hole can be respectively communicated with the plurality of liquid taking flow channels through the communicating flow channels and the communicating hole.
In the above technical solution, further, the sealing member is detachably connected to the second valve body.
In the above technical solution, further, the end cover is provided with a first extension portion facing the second valve body, and the first extension portion is formed with an initial position identification area.
In the above technical solution, further, the initial position identification area has an identification protrusion thereon.
In the above technical solution, further, a redundant opening is provided on the end cover and on one side of the identification protrusion;
when the second valve body rotates, the identification protrusion deforms and can be pressed to the redundant port.
In the above technical solution, further, the two pipetting valves are respectively a main pipetting valve and an auxiliary pipetting valve;
the two chambers are respectively a sample chamber and a re-dissolving chamber, the sample chamber is used for extracting a nucleic acid solution, and freeze-dried beads are stored in the re-dissolving chamber and used for carrying out re-dissolving reaction with the extracted nucleic acid solution;
the main liquid transfer valve can guide the extracted nucleic acid solution into the re-dissolving cavity and complete reagent re-dissolving in the re-dissolving cavity;
the auxiliary liquid-transferring valve can guide the reagent which completes the redissolution into the liquid inlet component.
In the above technical solution, further, the cartridge main body has a second extension portion, and the liquid inlet member is located at the second extension portion;
the liquid inlet component comprises a drainage needle, a capillary tube frame and a sealing cover plate;
the drainage needle is communicated with the auxiliary liquid transfer valve through the liquid taking flow channel, the capillary frame is sleeved on the drainage needle, and the sealing cover plate is buckled on the capillary frame;
the upper surface of the end part of the capillary tube frame is provided with a clamping part, and the lower surface of the end part of the capillary tube frame is provided with a groove.
In the above technical solution, further, the cartridge further includes a separable chamber member;
the separable chamber component comprises a union body and a mounting seat;
a part of reagent cavity is formed in the connecting pipe main body; a first limiting part is formed on one side, facing the connecting pipe main body, of the mounting seat; the first limiting part can clamp the header main body at a first position.
In the above technical solution, further, a second limiting portion is further formed on one side of the mounting seat facing the header body, and the second limiting portion is located below the first limiting portion;
the second limiting part can clamp the connecting pipe main body at a second position; when the connecting pipe main body moves from the first position to the second position, the cavity is communicated with the liquid taking flow passage.
In the above technical solution, further, the separable chamber component further includes a limiting member, and the limiting member has a clamping portion and a locking portion;
the clamping parts and the locking parts are respectively positioned at the end part of the connecting pipe main body and the end part of the mounting seat, and the clamping parts and the locking parts are in one-to-one correspondence;
when the union body moves to the second position, the locking part can be locked to the clamping part so as to lock the union body to the mounting seat.
In the above technical solution, further, a thin film layer is disposed at the bottom of the main body of the connecting pipe corresponding to the reagent chamber;
the bottom of the card box main body corresponds to the reagent cavity and is provided with a piercing part, when the clamping part is clamped and arranged at a position close to the bottom of the card box main body and the second limiting part, the piercing part pierces the thin film layer so as to enable the reagent cavity to be communicated with the liquid taking flow channel.
In the above technical solution, further, the cartridge further includes a housing; the housing comprises a base and an upper cover which can form an installation space;
the card box main body is fixed on the base;
the inside wall of upper cover corresponds the end cover is provided with first compact structure, corresponds the feed liquor component and is provided with second compact structure, corresponds separable cavity component is provided with third compact structure, so that feed liquor component, separable cavity component and end cover fastening with in the installation space.
The application also provides a sample processing device, which comprises a driving component and the card box;
the top edge of the second valve body is provided with connecting parts at intervals, and the driving assembly is provided with an abutting part and a driving part connected with the abutting part;
the connecting parts and the jacking parts are arranged in a one-to-one correspondence manner;
the top connection part can be inserted into the connection part; when the driving part is driven, the second valve body can be rotated in the first pipetting cavity through the abutting part and the connecting part.
In the above technical scheme, further, with an axis where a bottom center of a circle of the second valve body is located as a rotation axis, an angle at which the driving assembly drives the second valve body to rotate is the same as an included angle between adjacent liquid taking runners.
The application also provides a molecular diagnostic system comprising the sample processing device.
Compared with the prior art, the beneficial effect of this application is:
the application provides a card box, which comprises a card box main body and an end cover buckled on the card box main body; a plurality of chambers are formed in the cartridge body; the end cap is capable of sealing at least one of the plurality of chambers and is capable of communicating chambers of the plurality of chambers other than the sealed chamber with the cartridge.
The cartridge is applied to a rapid molecular detection system, and comprises a cartridge body and an end cover buckled on the cartridge body; a first pipetting cavity for placing a main pipetting valve, a second pipetting cavity for placing an auxiliary pipetting valve, a sample cavity for storing a sample, a redissolution cavity for storing freeze-dried beads, a waste liquid cavity for storing waste liquid and a plurality of reagent cavities are formed in the cartridge body; the end cover can cover the waste liquid cavity for storing waste liquid, so that the waste liquid in the waste liquid cavity is prevented from volatilizing, and the environment is prevented from being influenced; the end cover can cover the sample cavity for storing the sample, and after the sample is filled into the sample cavity, the end cover seals the sample cavity, so that the sample can be prevented from being polluted, and the detection accuracy is improved; wherein, the end cover switches on with first pipetting chamber and second pipetting chamber, and main pipetting valve and vice pipetting valve can extend from the end cover promptly, conveniently operate main pipetting valve and vice pipetting valve.
The application also provides a sample processing device, which comprises a driving component and the card box; the top edge of the second valve body is provided with connecting parts at intervals along the circumferential direction of the second valve body, and the driving assembly is provided with an abutting part and a driving part connected with the abutting part; the positions and the numbers of the connecting parts and the abutting parts are respectively arranged in a one-to-one correspondence manner.
Specifically, in the process of obtaining different reagents in different chambers or transferring the reagents by rotating the second valve body, the butting part is plugged into the connecting part; then drive the drive division, utilize rotatory drive division to through butt-joint part and connecting portion, thereby make the second valve body in first move liquid intracavity and rotate, adopt automatic rotation mode promptly, cancel manual rotation mode, it is more convenient.
The application also provides a molecular diagnostic system, comprising the sample processing device; based on the analysis of the sample processing device, the operation of the molecular diagnostic system is more convenient.
Drawings
In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings needed to be used in the detailed description of the present application or the prior art description will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of an end cap of a cartridge according to an embodiment of the present disclosure at a first viewing angle;
FIG. 2 is a schematic structural diagram of an end cap of a cartridge according to an embodiment of the present disclosure at a second viewing angle;
fig. 3 is a schematic structural diagram of a cartridge body of a cartridge provided in an embodiment of the present application at a first viewing angle;
FIG. 4 is a schematic structural diagram of a main body of a cartridge provided in an embodiment of the present disclosure at a second viewing angle;
FIG. 5 is a schematic structural diagram of a main body of a cartridge in a third viewing angle according to an embodiment of the present disclosure;
FIG. 6 is a schematic structural diagram of a main pipetting valve according to an embodiment of the present application;
FIG. 7 is a schematic structural diagram of a sealing member according to an embodiment of the present disclosure;
fig. 8 is a schematic structural diagram of a second valve body according to a first embodiment of the present disclosure;
FIG. 9 is a first structural schematic view of a seal member provided in accordance with an embodiment of the present disclosure;
FIG. 10 is a second schematic view of a sealing member according to an embodiment of the present disclosure;
fig. 11 is a schematic structural view of a limiting shaft according to an embodiment of the present disclosure;
fig. 12 is a schematic structural diagram of a housing according to an embodiment of the present application;
fig. 13 is a schematic structural diagram of an upper cover according to an embodiment of the present application;
fig. 14 is a schematic structural diagram of a base according to an embodiment of the present application;
fig. 15 is a schematic structural view of a hidden upper cover on a housing according to an embodiment of the present application;
fig. 16 is a schematic structural diagram of a sample processing device according to a second embodiment of the present application.
Reference numerals:
1-a cartridge body; 2-end cap; 3-a reagent chamber; 4-a first pipetting chamber; 5-a second pipetting chamber; 6-a sample chamber; 7-redissolution cavity; 8-waste liquid chamber; 9-a sealing member; 10-a first direction; 11-a second direction; 12-a sealing cover; 13-chamber lid; 14-a limiting shaft; 16-a handle; 18-a first guide; 19-a second guide; 20-a first sample inlet; 21-a second sample inlet; 22-a seal; 23-a primary pipetting valve; 24-a second valve body; 25-liquid taking hole; 26-conducting flow channel; 27-a via hole; 28-liquid taking flow channel; 29-a logo projection; 30-redundant ports; 31-secondary pipetting valve; 32-a liquid inlet member; 33-a second extension; 35-capillary shelf; 36-sealing the cover plate; 37-a clamping portion; 38-a groove; 39-a separable chamber component; 40-a union body; 41-a mounting seat; 43-a limiting part; 45-a stop; 46-a gripping portion; 47-a locking part; 48-a housing; 49-a base; 50-upper cover; 54-a connecting portion; 57-a positioning section; 58-a receiving part; 59-piston rod.
Detailed Description
The technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are only some embodiments of the present application, but not all embodiments.
The components of embodiments of the present application, generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
Example one
Cartridges according to some embodiments of the present application are described below with reference to fig. 1-15.
In this embodiment, a cartridge is provided, which is applied to a rapid molecular detection system, and comprises a cartridge body 1 and an end cap 2 fastened to the cartridge body 1; a plurality of chambers are formed in the cartridge body 1; the end cap 2 is capable of sealing at least one of the plurality of chambers and is capable of conducting the chambers of the plurality of chambers other than the sealed chamber to the outside of the cartridge.
Specifically, a first pipetting cavity 4 for placing a main pipetting valve 23, a second pipetting cavity 5 for placing a secondary pipetting valve 31, a sample cavity 6 for storing a sample, a re-dissolving cavity 7 for storing freeze-dried beads, a waste liquid cavity 8 for storing waste liquid and a plurality of reagent cavities 3 are formed in the cartridge body 1; the end cover 2 can cover the waste liquid cavity 8 for storing waste liquid, and the waste liquid in the waste liquid cavity 8 is prevented from volatilizing so as to prevent the waste liquid from influencing the environment; the end cover 2 can cover the sample cavity 6 for storing the sample, and after the sample cavity 6 is filled with the sample, the end cover 2 seals the sample cavity 6, so that the sample can be prevented from being polluted, and the detection accuracy is improved; the end cover 2 forms through holes corresponding to the first pipetting cavity 4 and the second pipetting cavity 5 and is communicated with the first pipetting cavity 4 and the second pipetting cavity 5, namely, the main pipetting valve 23 and the auxiliary pipetting valve 31 can extend out of the end cover 2, so that the main pipetting valve 23 and the auxiliary pipetting valve 31 can be operated conveniently; preferably, the end cap 2 forms a through hole corresponding to the reagent chamber 3 and is communicated with the reagent chamber 3, so as to facilitate pre-treatment of the reagent chamber 3 before pipetting, for example, directly performing puncture operation on the reagent chamber of the sealing film before pipetting.
Specifically, the card box further comprises a clamping piece, and the clamping piece is provided with a clamping part and a locking part; the clamping parts are arranged on the card box main body 1, the locking parts are arranged on the end cover 2, and the clamping parts correspond to the locking parts in position one to one; when downward force is applied to the end cover 2, the locking part can be clamped on the clamping part, so that the end cover 2 is tightly connected with the card box body 1, and the sealing effect on the sample cavity 6 and the waste liquid cavity 8 is further realized.
Specifically, a liquid taking flow channel 28 that can be communicated with the main liquid transfer valve 23 or the auxiliary liquid transfer valve 31 is opened at the bottom of the cartridge body 1 corresponding to each of the chambers (the first liquid transfer chamber 4, the second liquid transfer chamber 5, the sample chamber 6, the reconstitution chamber 7, the waste liquid chamber 8, and the reagent chamber 3), and a sealing plate is further provided at the bottom of the cartridge body 1 to seal the liquid taking flow channel 28 in the cartridge body 1.
In the actual operation process, after the sample is added into the sample cavity 6, the sample cavity cannot be opened again to prevent the sample from being polluted, and in order to conveniently realize the sample adding and sealing of the sample cavity 6, a sealing member 9 is arranged at the open end of the sample cavity 6.
Specifically, the sealing member 9 has an operation portion and a sealing portion connected to the operation portion; when the operating portion is driven in a first direction 10 (the first direction 10 herein may be understood as the width direction of the sample chamber 6 in a normal use state of the cartridge), the sealing portion can be opened or closed at the open end; further, when the sealing part is opened in the sample cavity 6 and leaks out of the open end of the sample cavity 6, the sample can be filled into the sample cavity 6; after the sample is filled, operating the operation part again, closing the sealing part to the open end of the sample cavity 6, and sealing the open end of the sample cavity 6; in conclusion, the filling and sealing of the sample chamber 6 are completed, and the sample in the sample chamber 6 can be prevented from being contaminated.
Specifically, in order to prevent sample contamination caused by the sealing portion being opened by a wrong operation of the operation portion during transportation or in the case of an improper operation, in this embodiment, after the operation of the sealing portion is completed in the first direction 10, the operation portion may be driven in the second direction 11 (the second direction 11 may be understood as the length direction of the sample chamber 6 in the normal use state of the cartridge) so that the operation portion covers the open end of the sample chamber 6 and is locked to the end cap 2, i.e., the sealing portion is secured to the open end of the sample chamber 6 and the sample chamber 6 is sealed.
In particular, the sealing member 9 comprises a chamber lid 13 on top of the end cap 2 and the sample chamber 6; a limiting shaft 14 is formed at one end of the chamber cover 13, and the limiting shaft 14 is positioned on a side plate surface of the chamber cover 13 facing the sample chamber 6; furthermore, a limit groove is formed in the side wall of the sample cavity 6, and one end of the limit shaft 14 can extend into the limit groove; a handle 16 capable of serving as the operating portion is formed at the other end of the chamber lid 13, and when the handle 16 is driven in the first direction 10, the stopper shaft 14 of the chamber lid 13 rotates in the stopper groove, so that the chamber lid 13 is opened or closed at the open end.
In particular, the sealing member 9 further comprises a sealing cover 12 located between the chamber cover 13 and the open end; the end cap 2 forms a guide hole through which the handle 16 can pass through the end cap 2; a first sample inlet 20 is formed on the end cover 2, and a second sample inlet 21 which is positioned on the same straight line with the first sample inlet 20 is formed on the sealing cover 12; when the chamber cover 13 is opened at the open end, the first sample inlet 20 is communicated with the second sample inlet 21.
Further, the guide hole comprises a first guide part 18, the first guide part 18 is arc-shaped, the center of the arc-shaped first guide part 18 is located on the axis of the limiting shaft 14, when the handle 16 is pulled, the handle 16 can be moved from the first end of the first guide part 18 to the second end of the first guide part 18, the chamber cover 13 rotates around the limiting shaft 14 by a preset angle, so that a part of the open end of the sample chamber 6 is exposed, and the sample chamber 6 is opened; so that the chamber lid 13 can be rotated to open or close the sample chamber 6 by reciprocating the trigger handle 16 in the first guide portion 18.
Further, the guide hole further comprises a second guide portion 19, the second guide portion 19 is in a strip shape, a first end of the second guide portion 19 is communicated with a first end of the first guide portion 18, and a second end of the second guide portion 19 extends along the second direction 11; the handle 16 is movable in the second direction 11 via the first end of the first guide 18 to the second end of the second guide 19, and when the handle 16 is moved to the second end of the second guide 19, the retainer shaft 14 is moved from the first end of the retainer groove to the second end of the retainer groove, so that the chamber cover 13 is locked to the open end.
In summary, when the handle 16 is located at the first end of the first guiding portion 18, the chamber cover 13 is located between the sealing cover 12 and the end cover 2, and the chamber cover 13 completely covers the sealing cover 12, so that the first sample inlet 20 and the second sample inlet 21 cannot be communicated through the blocking of the chamber cover 13, and since the chamber cover 13 is tightly pressed on the sealing cover 12 under the action of the end cover 2, the chamber cover 13 can seal and close the first sample inlet 20 on the sealing cover 12, so that the sample chamber 6 is in a sealed state. When the operating handle 16 moves from the first end of the first guide part 18 to the second end of the first guide part 18, the chamber cover 13 rotates by a predetermined angle around the limiting shaft 14, so that the chamber cover 13 blocked between the first sample inlet 20 and the second sample inlet 21 is moved away, the first sample inlet 20 on the sealing cover 12 is exposed, the sample chamber is opened, the second sample inlet 21 on the end cover 2 can be communicated with the sample chamber through the first sample inlet 20, and an operator can inject a sample into the sample chamber through the first sample inlet 20 and the second sample inlet 21; after the injection of the sample is completed, the handle 16 is pulled, so that the handle 16 returns to the first end of the first guide part 18 from the second end of the first guide part 18, the chamber cover 13 covers the first sample inlet 20 again to seal and seal the first sample inlet 20, so that the sample chamber is in a sealed state again, and the sample is prevented from leaking or diffusing to pollute; when the handle 16 is pulled to return the handle 16 from the first end of the second guide portion 19 to the second end of the second guide portion 19, the cover 13 will be locked to the cap 2, preventing the cover 13 from being opened by the wu's operation.
Further, a positioning portion 57 is provided on the side end surface of the cover 13 facing the end cap 2, a receiving portion 58 is provided on the side end surface of the cover 2 facing the cover 13, and when the handle 16 is returned from the first end of the second guide portion 19 to the second end of the second guide portion 19, the positioning portion 57 can be coincidently abutted on the receiving portion 58, so that the fixing of the sealing portion to the open end of the sample chamber 6 is ensured, and the sealing of the sample chamber 6 is ensured.
In this embodiment, a sealing layer is arranged on the top of a part of the reagent chamber 3, and the material of the sealing layer is preferably aluminum foil paper, and the sealing layer is packaged on the top of the reagent chamber 3 by using the aluminum foil paper, so that the part of the reagent chamber 3 forms a sealed chamber; when in use, the instrument can be used after puncturing.
In this embodiment, the cartridge further comprises a pipetting valve; the liquid transferring valve can be respectively communicated with the sample cavity 6, the re-dissolving cavity 7, the waste liquid cavity 8 and the plurality of reagent cavities 3, and the other end of the liquid transferring valve extends out of the end cover 2.
Specifically, the pipetting valve comprises a sealing element 22 (the sealing element 22 is preferably sealant), a piston rod 59, the piston rod 59 and a second valve body 24 detachably connected in the first pipetting cavity 4; specifically, the second valve body 24 is provided with a liquid taking port communicated with the valve cavity of the second valve body 24 and a conducting flow passage 26 communicated with the liquid taking port, one end of the piston rod 59 is positioned in the valve cavity of the second valve body 24, one end of the piston rod 59 positioned in the second valve body 24 is provided with a piston head, and the piston head is in sealing abutment with the valve cavity of the second valve body 24; the piston rod 59 can reciprocate in the second valve body 24 along the axis direction thereof, and pushes the piston rod 59 into the second valve body 24, so that the reagent in the second valve body 24 can be discharged through the liquid taking port of the second valve body 24 and the conducting flow passage 26; the piston rod 59 is pulled out of the second valve body 24, so that the reagent can be drawn into the second valve body 24 and introduced into the second valve body 24 through the liquid intake port and the conducting flow path 26.
Specifically, a plurality of liquid taking flow channels 28 are formed on the first liquid transferring cavity 4, and the plurality of liquid taking flow channels 28 are arranged at intervals along the circumferential direction; the sample cavity 6, the redissolution cavity 7, the waste liquid cavity 8 and the reagent cavities 3 are respectively communicated with the first liquid transferring cavity 4 through the liquid taking flow channel 28; furthermore, one end of the second valve body 24, which is formed with a liquid taking port, is movably connected in the first liquid transferring cavity 4, and the second valve body 24 can move in the first liquid transferring cavity 4, so that the liquid taking port and the conducting flow channel 26 of the second valve body 24 are communicated with the liquid taking flow channel 28 of the first liquid transferring cavity 4, and further the second valve body 24 is communicated with any one of the sample cavity 6, the reconstitution cavity 7, the waste liquid cavity 8 and the plurality of reagent cavities 3; so that when the operator pulls the piston rod 59 out of the second valve body 24, the reagent in any one of the sample chamber 6, the reconstitution chamber 7, the waste liquid chamber 8 and the plurality of reagent chambers 3 can be drawn into the second valve body 24, and when the operator pushes the piston rod 59 into the second valve body 24, the reagent in the second valve body 24 can be transferred into any one of the sample chamber 6, the reconstitution chamber 7, the waste liquid chamber 8 and the plurality of reagent chambers 3.
More specifically, a bottom wall (i.e., the bottom wall of the second valve body 24) is formed at one end of the second valve body 24 located in the first liquid moving chamber 4, the liquid taking port is formed at the bottom wall of the second valve body 24, one end of the conducting flow passage 26 is communicated with the liquid taking hole 25, and the other end extends along a straight line direction where the liquid taking hole 25 passes through a center of the second valve body 24 (it can also be understood that the other end extends toward a circumferential direction of the bottom wall of the second valve body 24). The first pipetting cavity 4 is provided with a bottom wall (namely the bottom wall of the first pipetting cavity 4), and the liquid taking flow channel 28 is formed on the bottom wall of the first pipetting cavity 4; the bottom wall of the second valve body 24 is clamped with sealant, and when the second valve body 24 moves in the first liquid transferring cavity 4, the sealant can move synchronously with the second valve body 24; the sealant is provided with a through hole 27, and when the second valve body 24 is arranged in the first liquid transferring cavity 4, the sealant can be clamped between the bottom wall of the first liquid transferring cavity 4 and the bottom wall of the second valve body 24, so that the first liquid transferring cavity 4 and the second valve body 24 are in sealing abutting through the sealant; when the second valve body 24 is moved to a predetermined position (a predetermined position in this case is understood to be a position communicating with one of the sample chamber 6, the reconstitution chamber 7, the waste liquid chamber 8, and the plurality of reagent chambers 3), the communicating hole 27 can communicate with the liquid taking flow channel 28 of the first pipetting chamber 4, and then communicate with the second valve body 24 through the communicating flow channel 26 and the liquid taking port.
It is worth noting that: because the sealant is detachably connected with the second valve body 24, compared with the existing sealing mode, for example, the end face sealing is adopted between the first liquid transferring cavity 4 and the second valve body 24, or the second valve body 24 is encapsulated, the sealant is detachably connected onto the second valve body 24, so that the production cost is saved, the size of the sealant can be changed, and the second valve body 24 can be driven to rotate in the first liquid transferring cavity 4 more easily while the sealing performance between the first liquid transferring cavity 4 and the second valve body 24 is ensured.
More noteworthy is that: the conducting flow channel 26 is formed at the bottom of the second valve body 24 instead of the sealant, so that the extrusion of the reagent to the sealant is reduced, and the sealant can be prevented from being deformed.
In this embodiment, the end cap 2 is provided with a first extension toward the second valve body 24, the first extension having an initial position identification area formed thereon; specifically, the initial position identification area is provided with an identification protrusion, and the initial position of the second valve body 24 is identified by using the identification protrusion 29, so that the second valve body 24 can be returned to the initial position every time the molecular detection is completed, and the next molecular detection is facilitated.
Preferably, the identification protrusion 29 is made of soft plastic and can be slightly deformed.
Specifically, a redundant opening 30 is formed in one side of the end cover 2, which is located on the identification protrusion 29; specifically, when the second valve body 24 rotates, a small deformation of the identification protrusion 29 will be extruded into the redundant port 30, so as to avoid affecting the rotation of the second valve body 24 in the first pipetting cavity 4.
In this embodiment, two pipetting valves are provided, namely a main pipetting valve 23 and an auxiliary pipetting valve 31 (the main pipetting valve 23 and the auxiliary pipetting valve 31 have the same structure and are not described herein, and the main pipetting valve 23 and the auxiliary pipetting valve 31 are non-conductive); in the actual use process: firstly, nucleic acid extraction needs to be completed in the sample cavity 6; then the main liquid transfer valve 23 extracts the reagent for completing the nucleic acid extraction into the second valve body 24, and then the qPCR reaction system freeze-dried beads in the re-dissolution cavity 7 can perform re-dissolution reaction with the reagent for completing the nucleic acid extraction when the main liquid transfer valve is pushed into the re-dissolution cavity 7; finally, the auxiliary pipetting valve 31 draws the re-dissolved reagent from the re-dissolving cavity 7 and introduces the re-dissolved reagent into the liquid inlet component 32; in conclusion, the auxiliary pipetting valve 31 can be respectively communicated with the reconstitution cavity 7 and the liquid inlet member 32, so that the space of the cartridge is saved, and the structure is more compact.
In this embodiment, the cartridge body 1 has a second extension 33, and the liquid inlet member 32 is located at the second extension 33; specifically, the second extension 33 extends from the cartridge body 1 to the outside of the cartridge body 1 on the side close to the sub-pipette valve 31.
The liquid inlet component 32 comprises a drainage needle, a capillary frame 35 and a sealing cover plate 36; the drainage needle is communicated with the auxiliary liquid transfer valve 31 through the liquid taking flow channel 28, the capillary tube frame 35 is sleeved on the drainage needle, and the sealing cover plate 36 is buckled on the capillary tube frame 35; a clamping part 37 is formed on the upper surface of the end part of the capillary tube frame 35, and a groove 38 is formed on the lower surface; specifically, in the process of transferring the liquid inlet member 32, a first transfer portion of the transfer mechanism (the transfer mechanism is a prior art, and not described herein too much) is clamped with the clamping portion 37, and a second transfer portion of the transfer mechanism is clamped with the groove 38, that is, the clamping portion 37 and the groove 38 are used to realize bidirectional positioning with the transfer mechanism, so that the stability of transferring the liquid inlet member 32 is ensured, and when the transfer mechanism clamps the liquid inlet member 32 and rotates 360 °, the liquid inlet member 32 can be prevented from falling off the transfer mechanism.
In this embodiment, the cartridge further comprises a detachable chamber member 39; the separable chamber member 39 includes a main body 40 and a mounting seat 41 having a liquid-taking flow passage 28 at the bottom; a plurality of reagent chambers 3, preferably four reagent chambers 3, are formed in the header body 40; in order to avoid the reaction liquid, especially the reaction liquid with high ion concentration, contacting the first liquid transfer chamber 4 for a long time to generate crystallization or corrosion, in the embodiment, the side wall of the connecting pipe main body 40 is provided with a clamping part; the mounting seat 41 is formed on the card box main body 1, and two limiting parts 43 are formed on the side wall of the mounting seat at intervals in the vertical direction corresponding to the clamping parts; in the transportation process or in an unused state, the clamping portion is clamped on the limiting portion 43 (the limiting portion 43 inside is a first limiting portion) far away from the bottom of the card box body 1, that is, the connecting tube body 40 and the mounting seat 41 are not conducted (that is, the connecting tube body is located at the first position), and the reaction liquid in the reagent chamber 3 does not contact the first liquid transferring chamber 4 at this time, so that the first liquid transferring chamber 4 is not corroded.
When the reaction liquid in the reagent chamber 3 needs to be used, the connecting tube main body 40 is pressed downward (a pressing mechanism, which is a conventional technique and not described herein too much), so that the clamping portion of the connecting tube main body 40 is clamped to the position-limiting portion 43 (the position-limiting portion is the second position-limiting portion) near the bottom of the cartridge main body 1, the chamber is conducted to the liquid-taking flow channel 28, and the connecting tube main body can be taken by the main liquid-moving valve 23 (that is, the connecting tube main body is located at the second position).
According to the above explanation, in order to ensure that when the clamping part is clamped on the limiting part 43 near the bottom of the cartridge main body 1, the reaction liquid in the reagent chamber 3 can be taken out by the main liquid-moving valve 23 through the mounting seat 41, and the sealing performance and the conductivity of the separation chamber component are ensured; the separable chamber component 39 further comprises a retainer 45, the retainer 45 having a gripping portion 46 and a locking portion 47; the clamping parts 46 are positioned at the end part of the header body 40 along the length direction, the locking parts 47 are positioned at the end part of the mounting seat 41 along the length direction, and the clamping parts 46 correspond to the locking parts 47 in position one to one; when the clamping part is clamped at the position close to the limiting part 43 at the bottom of the card box body 1, the locking part 47 can be locked on the clamping part 46 so as to lock the pipe connecting body 40 on the mounting seat 41, and the sealing property between the pipe connecting body 40 and the mounting seat 41 is further ensured.
Specifically, the bottom of the header body 40 is provided with a thin film layer corresponding to the chamber; the bottom of the card box body 1 is provided with a piercing part corresponding to the reagent cavity 3, and when the clamping part is clamped at the position close to the limiting part 43 at the bottom of the card box body 1, the piercing part pierces the film layer so as to enable the cavity to be communicated with the liquid taking flow channel 28.
In this embodiment, to ensure that the cartridge is not subject to leakage and contamination problems during transport, the cartridge further comprises a housing 48; the housing 48 includes a base 49 and an upper cover 50 fastened on the base 49, and an installation space is formed between the base 49 and the upper cover 50; the cartridge body 1 is fixed to the base 49; the inside wall of upper cover 50 corresponds end cover 2 is provided with first compact structure, corresponds feed liquor component 32 is provided with second compact structure, corresponds separable cavity component 39 is provided with the third compact structure, so that feed liquor component 32, separable cavity component 39 and end cover 2 fastening with in the installation space.
First compact structure, second compact structure and third compact structure are preferred from the inside wall orientation of upper cover 50 first locating plate, second locating plate and the third locating plate that extends on the end cover 2, the direct butt of the other end of first locating plate is on end cover 2, the direct butt of the other end of second locating plate is at the top of feed liquor component 32, the direct butt of the other end of third locating plate is at the top of separation cavity component, when upper cover 50 lock is on base 49 promptly, first locating plate can compress tightly end cover 2 on card box main part 1, the second locating plate can compress tightly feed liquor component 32 on card box main part 1, the third locating plate can compress tightly the separation cavity component on card box main part 1.
Specifically, the base 49 and the cover 50 have interlocking structures thereon to ensure that the base 49 and the cover 50 can be fixedly connected.
Example two
As shown in fig. 16, the present application also provides a sample processing device comprising a drive assembly and the cartridge described above; the top edge of the second valve body 24 is provided with connecting parts 54 at intervals along the circumferential direction of the second valve body 24, and the driving assembly is provided with an abutting part and a driving part connected with the abutting part; the positions and the number of the connecting parts 54 and the abutting parts are respectively arranged in a one-to-one correspondence manner; the top connection part is inserted into the connection part 54 in the process of obtaining different reagents in different chambers or transferring the reagents by rotating the second valve body 24; then, the driving part is driven, the second valve body 24 rotates in the first liquid transferring cavity 4 through the rotary driving part and the abutting part and the connecting part 54, namely, an automatic rotating mode is adopted, and a manual rotating mode is omitted, so that the liquid transferring device is more convenient and fast.
In this embodiment, an axis where a center of a circle of the bottom of the second valve body 24 is located is taken as a rotation axis, and an angle at which the driving assembly drives the second valve body 24 to rotate is the same as an included angle between adjacent liquid taking channels 28; in the actual operation process, an operator can determine which chamber to conduct according to the rotating angle of the driving assembly, so that the reagent is convenient to transfer, extract and the like.
EXAMPLE III
The application also provides a molecular diagnostic system comprising the sample processing device. Based on the above analysis of the sample processing device, the molecular diagnostic system has the advantages of simple structure and convenient operation.
Finally, it should be noted that: the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.
Claims (26)
1. The card box is applied to a rapid molecular detection system and is characterized by comprising a card box body and an end cover buckled on the card box body;
a plurality of chambers are formed in the cartridge body;
the end cap is capable of sealing at least one of the plurality of chambers and is capable of communicating chambers of the plurality of chambers other than the sealed chamber with the cartridge.
2. The cartridge of claim 1, wherein an open end of at least one of the chambers sealed by the end cap is provided with a sealing member;
the sealing member has an operating portion and a sealing portion connected to the operating portion;
when the operation part is driven along a first direction, the sealing part can be opened or covered at the open end;
when the operation portion is driven in a second direction, the seal portion can be locked or unlocked to the open end.
3. The cartridge of claim 2, wherein the sealing member comprises a chamber cover configured as the sealing portion;
a handle capable of serving as the operating part is formed at one end of the chamber cover, and the handle is positioned on one side plate surface of the chamber cover facing the end cover; when the handle is driven along the first direction, the chamber cover can be opened or covered at the open end.
4. The cartridge of claim 3, wherein the other end of the chamber cover is formed with a stopper shaft on a side plate surface of the chamber cover facing the chamber; the side wall of the cavity is provided with a limiting groove, and the limiting shaft can extend into the limiting groove;
the retainer shaft of the chamber lid is rotatable within the retainer slot when the handle is driven in the first direction.
5. The cartridge of claim 4, wherein the end cap has a guide hole formed therein through which the handle can pass through the end cap;
the end cover is provided with a first sample inlet, and when the cavity cover is opened at the open end by driving the handle, the first sample inlet is communicated with the cavity.
6. The cartridge of claim 5, wherein the sealing member further comprises a sealing cover between the chamber lid and the open end;
a second sample inlet which is positioned on the same straight line with the first sample inlet is formed on the sealing cover;
when the chamber cover is opened at the open end, the first sample inlet is communicated with the second sample inlet.
7. The cartridge of claim 5, wherein the guide hole comprises a first guide portion and a second guide portion;
the handle can move from the first end of the first guide part to the second end of the first guide part along the first direction so as to enable the chamber cover to rotate around the limiting shaft by a preset angle; when the handle is located at the second end of the first guide portion, the chamber is opened;
the first end of the second guide part is communicated with the first end of the first guide part, and the second end of the second guide part extends along the second direction; the handle can move to the second end of second guide part along the second direction via the first end of first guide part, and when the handle moved to the second end of second guide part, spacing axle by the first end of spacing groove moves to the second end of spacing groove, so that the chamber lid locks in the open end.
8. A cartridge according to claim 1, wherein the top of the chamber, which is partly in communication with the outside of the cartridge, is provided with a sealing layer, so that the chamber forms a sealed chamber.
9. The cartridge of claim 1, wherein one of the chambers is a first pipetting chamber, at least one of the chambers is a reagent chamber, and a bottom of the first pipetting chamber is provided with a liquid taking flow channel through which the first pipetting chamber can be communicated with the reagent chamber;
the cartridge further comprises a pipetting valve; one end of the liquid transferring valve can be communicated with the first liquid transferring cavity.
10. The cartridge of claim 9, wherein the pipetting valve comprises a seal and a second valve body connected within the first pipetting chamber;
the sealing member is connected with the second valve body, and the second valve body is sealed against the first pipetting cavity through the sealing member;
a liquid taking hole and a conducting flow passage communicated with the liquid taking hole are formed at the bottom of the second valve body; the sealing element is provided with a conducting hole communicated with the conducting flow channel;
when the second valve body rotates in the first pipetting cavity, the sealing element moves synchronously with the second valve body, and the liquid taking hole is communicated with the liquid taking flow channel through the communicating flow channel and the communicating hole in sequence.
11. The cartridge of claim 10, wherein one end of the conducting flow path communicates with the liquid taking hole, and the other end extends in a direction of a straight line where the liquid taking hole passes through a center of the second valve body.
12. The cartridge of claim 10, wherein the liquid extraction flow channel is provided in plurality, and the plurality of liquid extraction flow channels are arranged at intervals in a circumferential direction;
when the second valve body and the sealing piece synchronously rotate in the first liquid transferring cavity, the liquid taking hole can be respectively communicated with the plurality of liquid taking flow channels through the communicating flow channels and the communicating hole.
13. The cartridge of claim 10, wherein the sealing member is removably coupled to the second valve body.
14. The cartridge of claim 10, wherein the end cap is provided with a first extension part toward the second valve body, the first extension part having an initial position recognition area formed thereon.
15. The cartridge of claim 14, wherein the home position identification area has an identification protrusion thereon.
16. The cartridge of claim 15, wherein the end cap defines a redundant opening on a side of the identification protrusion;
when the second valve body rotates, the identification protrusion deforms and can be pressed to the redundant port.
17. The cartridge of claim 14, wherein the pipetting valve is provided with two, respectively a primary pipetting valve and a secondary pipetting valve;
the two chambers are respectively a sample chamber and a re-dissolving chamber, the sample chamber is used for extracting a nucleic acid solution, and freeze-dried beads are stored in the re-dissolving chamber and used for carrying out re-dissolving reaction with the extracted nucleic acid solution;
the main liquid transfer valve can guide the extracted nucleic acid solution into the re-dissolving cavity and complete reagent re-dissolving in the re-dissolving cavity;
the auxiliary liquid-transferring valve can guide the reagent which completes the redissolution into the liquid inlet component.
18. The cartridge of claim 17, wherein the cartridge body has a second extension, the liquid inlet member being located at the second extension;
the liquid inlet component comprises a drainage needle, a capillary tube frame and a sealing cover plate;
the drainage needle is communicated with the auxiliary liquid transfer valve through the liquid taking flow channel, the capillary frame is sleeved on the drainage needle, and the sealing cover plate is buckled on the capillary frame;
the upper surface of the end part of the capillary tube frame is provided with a clamping part, and the lower surface of the end part of the capillary tube frame is provided with a groove.
19. The cartridge of claim 17, further comprising a detachable chamber member;
the separable chamber component comprises a union body and a mounting seat;
a part of reagent cavity is formed in the connecting pipe main body; a first limiting part is formed on one side, facing the connecting pipe main body, of the mounting seat; the first limiting part can clamp the header main body at a first position.
20. The cartridge of claim 19, wherein a second position-limiting portion is further formed on one side of the mounting seat facing the main body of the header, and the second position-limiting portion is located below the first position-limiting portion;
the second limiting part can clamp the connecting pipe main body at a second position; when the connecting pipe main body moves from the first position to the second position, the cavity is communicated with the liquid taking flow passage.
21. The cartridge of claim 20, wherein the separable chamber component further comprises a retainer having a gripping portion and a locking portion;
the clamping parts and the locking parts are respectively positioned at the end part of the connecting pipe main body and the end part of the mounting seat, and the clamping parts and the locking parts are in one-to-one correspondence;
when the union body moves to the second position, the locking part can be locked to the clamping part so as to lock the union body to the mounting seat.
22. The cartridge of claim 20, wherein the bottom of the manifold body is provided with a thin film layer corresponding to the reagent chamber;
the bottom of the card box main body corresponds to the reagent cavity and is provided with a piercing part, when the clamping part is clamped and arranged at a position close to the bottom of the card box main body and the second limiting part, the piercing part pierces the thin film layer so as to enable the reagent cavity to be communicated with the liquid taking flow channel.
23. The cartridge of claim 20, further comprising a housing; the housing comprises a base and an upper cover which can form an installation space;
the card box main body is fixed on the base;
the inside wall of upper cover corresponds the end cover is provided with first compact structure, corresponds the feed liquor component and is provided with second compact structure, corresponds separable cavity component is provided with third compact structure, so that feed liquor component, separable cavity component and end cover fastening with in the installation space.
24. A sample processing device comprising a drive assembly and a cartridge according to any one of claims 10 to 23;
the top edge of the second valve body is provided with connecting parts at intervals, and the driving assembly is provided with an abutting part and a driving part connected with the abutting part;
the connecting parts and the jacking parts are arranged in a one-to-one correspondence manner;
the top connection part can be inserted into the connection part; when the driving part is driven, the second valve body can be rotated in the first pipetting cavity through the abutting part and the connecting part.
25. The sample processing device according to claim 24, wherein an axis on which a center of a circle of a bottom of the second valve body is located is taken as a rotation axis, and an angle at which the driving assembly drives the second valve body to rotate is the same as an included angle between adjacent liquid taking flow channels.
26. A molecular diagnostic system, comprising the sample processing device of claim 25.
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| CN202111480621.0A CN113980776B (en) | 2021-12-06 | 2021-12-06 | Cartridge, sample processing device, and molecular diagnostic system |
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| CN202111480621.0A CN113980776B (en) | 2021-12-06 | 2021-12-06 | Cartridge, sample processing device, and molecular diagnostic system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2023151243A1 (en) * | 2022-02-08 | 2023-08-17 | 江苏液滴逻辑生物技术有限公司 | Lyosphere pre-embedding structure, digital microfluidic chip, and pre-embedding and liquid injection method |
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